Preface The pressure of an ever-increasing population and periodic famine due to unexpected flood and drought has forced and awakened the horticultural scientist, to evolve new plant types for diversified use. Besides, some limitations in the improvement of fruit crop such as long juvenile phase, high heterozygosity, limited information on inheritance pattern, excessive fruit drop, parthenocarpy and lesser number of seeds per fruit, hybridization, selection, mutation and other tools of fruit breeding have resulted in the development of a number of varieties in mango, grape, papaya, banana and guava for various purposes. A vast literature is available on every aspects of fruit cultivation and many excellent books and manuals have been written from time to time however, compiled information on fruit breeding are not available under a single head. The present book entitled “ Fruit Breeding Approaches and Achievements” Revised and enlarged edition is ventured with the objective to provide latest possible information on basic approaches in fruit breeding, breeding for biotic stresses resistance, use of plant growth regulators in fruit improvement, improvement of important fruit crops such as mango, banana, papaya, grape, guava, citrus, ber, aonla, pomegranate, date palm, litchi, coconut, cashewnut, pineapple, few under utilized fruits and temperate fruits in a broad spectrum. It is with more than mere formality we thank Dr. A.K. Singh, Deputy Director General (Horticultural Sciences), ICAR, New Delhi for inspiration given by them in writing this book. Authors express their deep sense of gratitude to Dr. O.P. Yadav, Director, Central Arid Zone Research Institute, Jodhpur for giving more thrust to increase quality publications. We are also thankful to Dr. Dipak Kumar Gupta and Dr. R.S. Mehta for their critical comments and suggestions. Authors are thankful to All Scientist and workers of Central Arid Zone Research Institute, Regional Research Station, Pali (Rajasthan) for their help during writing this book. Authors books, book chapters, research papers, popular articles and technical papers used in writing of this book have duly been acknowledged in the text. The authors will appreciate receiving suggestions for further improvement of this book. We hope that information presented here in the book would receive due response from the teaches, students, researchers and other readers

India is bestowed with a wide range of agro-climatic and soil conditions. Therefore, almost all types of fruits can be grown in one or the other parts of the country. India is the second largest producer of fruits next to China. In India, fruit crops occupied 6.5 million hectares and it produces 96.75 million metric tonnes of fruits. India has exported fruits worth Rs. 4817.35 crores (692.01 USD Millions) (National Horticulture Board). Improvement of fruit crops is difficult because of long gestation period, high heterozygosity, scanty information on inheritance pattern, often cross pollination, excessive fruit drop, parthenocarpy and less number of seeds restricting the availability of hybrid seedlings for evaluation. Even though, planned hybridization and clonal selections have been attempted in a number of fruit crops and these efforts have resulted in the development of promising varieties (mango, grape, guava, papaya, sapota, banana, etc). However, systematic and dedicated efforts are still required for the development of ideal varieties through modern tools in fruit crops. In future, search for desired gene, critical study of inheritance pattern and use of biotechnological tools require due attention in combining ideal characteristics in varietal improvement programme of fruit crops.

A plant is said to be healthy or normal when it carries out its physiological functions to the best of its genetic potential. These normal functions include division, differentiation, development, absorption of water and mineral from soil and translocation of these throughout the plants, photosynthesis and translocation of photosynthetic product to areas of utilization or storage, the metabolism of synthesized compounds, reproduction and storage of food supplies. A plant becomes diseased when it is disturbed by pathogen under certain environmental conditions which interfere with one or more of its essential functions. Diseased plant refers to any disturbance brought about by living organism under environmental factors, which interfere with normal function of plant or in other words when any organ and part of plant is not doing their work properly and when either the growth or reproduction is not going forward in natural or regular manner.

Plant growth regulators are organic substances other than nutrient, which is required in minute quantity to modify the plant growth processes. PGR has very important role in fruit breeding which is used in every facet of crop improvement e.g. multiplication of hybrids/selection through asexual means or by micro propagation, breaking of seed dormancy, growth control, regulation of flowering, fruiting and induction of sterility in reproductive organs etc (Dhatt, 1985). Details of the various uses of plant growth regulators are given as under.

Mango is one of the choicest fruits of India, grown over an area of 1.23 million hectares in the country. Mango occupies the same position in India as occupied by the apple in temperate and grape in subtropical areas. In India mango is acclaimed as king of fruits. The name Mangifera was given for the first time by Bontius in 1658, when he referred to this plant as arbor Mangifera (The tree producing mango). Linnaeus also referred to it as Mangifera arbor in 1747, prior to changing the name to its present form (Mangifera indica) in 1753. Mango is good source of vitamin A and C apart from the usual content of minerals and other vitamins. Mango is also considered to have some medicinal properties. Ripe fruits of mango is fattening, diuretic and laxative. Smoke of burning is supposed to cure hiccups and some throat troubles and the kernal is effective against diarrhoea and asthma. Besides table purpose, fruits of mango can be used for the preparation of pickles, preserves, jam, amchur (mango powder), mango leather (ampapad) and mango fool (mango+milk+sugar)

Banana is one of the oldest fruits and second largest growing fruit crop in the world. It is also known as the Adam’s Fig and Apple of Paradise. Unlike other fruits banana is an herbaceous fruit plant. Banana breeding started in Trinidad, West Indies in 1922 and in Jamaica in 1924 (Shepherd, 1994). The driving force for this breeding programme was to develop improved Fusarium wilt (Fusarium oxysporum F. sp. Cubense) resistant banana for export trade (Buddenhagen, 1990). In 1960 both the programmes were combined under the Jamaica Banana Board. United Fruit Company also started a small breeding programme in Panama in 1920s. In India hybridization work was started at Central Banana Research Station, Aduthurai, Tamil Nadu in 1949. Important banana growing states are Maharashtra, Karnataka, Kerala, Tamil Nadu, Andhra Pradesh, Orissa, Bihar, West Bengal and Assam. Now a days in some districts of Uttar Pradesh, Harichal cultivar of banana is cultivated at commercial scale. In South India banana is extensively used in all auspicious occasions such as wedding, festival and worship. Banana is a good table fruit, besides, the cultivar Nendran is used for cooking, it is also used for preparation of halwa, sweet and chips etc. Main bud or heart of banana bunches is also used as vegetable in India, Thailand, Philippines, Malaysia and Indonesia. Musa textiles is known for strong fibre quality.

Papaya is an ideal fruit crop for growing in kitchen garden, backyards of home as well as orchards, especially those places nearer to cities or big town. It is also grown as a filler plant in orchard. The ripe fruits of papaya are consumed throughout the tropics and sub tropics. Fruits are also used in preparation of jam, soft drinks, ice cream flavour, crystalized fruits and syrups. Unripe fruits are commonly used as vegetables. Papain is prepared from the latex of immature fruits. It is proteolytic enzyme used for tendering of the meat, preparation of chewing gum and cosmetics etc. The ripe fresh fruit is rich source of vitamin-A (2500 I.U.), carbohydrates and minerals (Singh, 1969). Papaya on account of producing fruit in a short period after planting has attracted the attention of fruit growers to be cultivated on a large scale in the country. It is known as wholesome fruit which is valued for its nutritional and medicinal properties. Due to its nutritional, industrial and export demand, papaya is recognized the most potential fruit crop for its economic cultivation. The variable sex forms, propagational problems, susceptibility to frost and water logging, fungal and viral diseases are well identified problems in its cultivation. Economically the production life of papaya is 3 years and every time new plantation has to be raised. Papaya being transplanted at closer spacing is highly suitable for high density planting. Now high yielding variety with better fruit quality are needed to boost the production under varied agro edaphological situation.

Citrus constitutes a major group of fruits comprising of mandarins, oranges, lemon, pummelo, grape fruits, tangelo, trifoliate orange, citron, citranges etc. Despite of inter-specific and inter-generic hybrids, Poncirus and Fortunella also belong to genus Citrus. During its long history, citrus has given the world numerous varieties both by open pollination, bud sports and of recently by controlled pollination and artificial induction of bud variation. Citrus fruit cultivation lies between latitude 40 0N also 40 0S where conditions are neither cold nor moist and dry. India is considered to be the home of several citrus species and they are found growing wild in some parts of the country. Many types of citrus still remain unexploited by man and such types are considered as semi wild. The productivity of citrus plant in our country is very low which is 7.65 tonnes/ha as acompared to Spain, Italy and Japan where it lies between 17-35 tonnes/ha. Sweet oranges cover an area of 42.4 thousand ha with an annual production of 510.8 thousand tonnes (Ghosh, 1985).

Grapes are being grown commercially in the tropics. The region between the tropics of Cancer and Capricorn, which was once considered to be unsuitable for growing quality grapes (Winkler, 1962) is producing good quality grapes. Tropical countries, where grapes are grown are South–East Asia (India, Indonesia, Philippines, Thailand, Taiwan, South China and Australia), Central and South America (Brazil, Columbia, Ecuador, Mexico, Venezuela and West Indies) and Africa (Kenya, Nigeria and Zimbabwe). In India, about 90% of grapes are being produced in the tropical region of the peninsular states i.e. Maharashtra, Andhra Pradesh, Karnataka and Tamil Nadu. However, now-a-days grape is becoming popular in North India due to good income. The vines of tropical region grape are evergreen in nature and do not shed their leaves. In this condition, pruning is done twice in a year during April and September–October. But under North Indian conditions, grapes shed their leaves during winter and pruning is done in the month of February.

Guava is also known as the “Apple of the Tropics”. It is very rich and cheap source of vitamin C and also contains a fair amount of calcium. Important guava growing states in the country are Uttar Pradesh, Bihar, Madhya Pradesh and Maharashtra. Allahabad district of Uttar Pradesh has the reputation of growing the best quality of guava fruits in the world (Mitra and Bose, 1990). The importance of guava is due to the fact that it is the hardy fruit which can be grown in alkaline and poorly drained soil.

Ber (chinese date, chinese fig or bore) is an important minor fruit of India. It is hardy fruit tree cultivated all over India and also known as poor man’s fruit. It is most widely cultivated in Punjab, Haryana, Rajasthan, Uttar Pardesh, Madhya Pradesh, Bihar, Gujarat, Maharashtra etc. Ber can do well even under marginal growing conditions and provides quality yields at low cost (Shukla, 1996). Fruits are mostly eaten fresh but the other forms, such as dried, candied, squash etc can also be prepared from ber. Stem bark, root and leaves have some medicinal values. Leaves of ber are used as fodder in dry regions. Ber tree (Ziziphus xylopyrus) is a host plant for rearing the Tachardia laccad a lac insect (Sharma and Kore, 1990).

Aonla has been regarded as a sacred tree in India. The tree is worshipped as mother earth and is believed to nurture humankind because the fruits are very nourishing. It is also known as Amrit phal. Aonla is mainly recognized due to its high nutritive, medicinal and therapeutic properties. It contains high amount of vitamin C (500-1500 mg/100 g), it is also rich in pectin, iron, calcium and phosphorous. Dried fruits of aonla can be is used in curing hemorrhage, diarrhea, chronic dysentery, diabetes, jaundice, dyspepsia, cough etc. Recently, aonla has gained more popularity in arid and semi-arid region of the country because of its hardy nature, prolific bearing capacity and ability to grow under various adversities as a remunerative crop (Shukla et al.2002). It may be an important fruit in future due to its high productivity, suitability in wasteland particularly salt affected soil and sandy soil of arid ecosystem. Aonla is the main ingredient of Chavanprash and Triphala powder which is used for curing different abnormalities. The fruit contains a chemical substance gallic acid and leucoanthocyanin which have antioxidant property.

Pomegranate is an ancient and important fruit. It is grown for its delicious and juicy pink arils (outer growth of seed) which are eaten fresh (Keskar et al., 1993). Fresh fruit is of exquisite quality while its processed products like bottled juice, syrups and jelly are highly appreciated. The juice is considered useful for patients suffering from leprosy. Fruit juice easily fermented and may be used for the production of wine. Juice of wild pomegranate is used in the manufacture of citric acid and sodium citrate for medicinal purpose. Pomegranate is known best for curing the chronic stomach ailment. Seeds of pomegranate contain oil which has a potential for industrial use. Sour aril of the wild types can be utilized for the preparation of anardana.

Date palm is one of the oldest fruits and was known to man many thousand years before Christ. It is staple food of the Arabs. Large quantities of date are imported by India from the Arabian countries. Wild date palm are seen growing even up to an altitude of 1500 M (Pareek, 1990), of course they do not fruit at this altitude every year. Raw sugar and a fermented drink ‘toddy’ are prepared from the sap of this palm. In the Indians valley, date palm is believed to have been introduced by the soldiers of Alexander the great in the fourth century B.C. and some wild groves of seedling date palm are found in the coastal belt in the Kachchh district of Gujarat. Investigations to develop indigenous production of dates in India were initiated during the 1950 by the ICAR at Regional Fruit Research Station, Abohar (Punjab). Some commercial varieties of date palm were introduced from USA, Pakistan and Middle East countries during 1955-1962. The extremely dry areas comprising Jaisalmer, Barmer, Bikaner and Jodhpur in Rajasthan, Abohar in Punjab and Kachchh in Gujarat are the potential regions for the date growing in India.

Litchi is a delicious fruit and generally consumed as a table fruit. It is one of the most popular fruits of India which is commonly taken as fresh or in dried forms. Litchi is also recognized as “Queen of the fruits”. Dried litchi commonly known as ‘Litchi nut’ is very popular among the Chinese living all over the world. This subtropical fruit cannot tolerate frost in winter and dry heat in summer. An interesting feature of the litchi plant is the symbiotic growth of Mycorrhiza fungi on roots of the tree. These fungi form nodules on the roots of litchi. The trees and the fungus live together to benefit each other. Litchi makes an excellent canned fruit and highly flavoured squash is prepared from inferior fruits. Besides, fruit, other parts of the plant like leaves, bark and roots are also used for various purposes. In China leaves are used for making poultices, bark and roots for making decoctions for throat gargle.

Coconut is a monocotyledonous woody plant belonging to the family Arecaceae or Palmae. It is presumed that the generic name Cocos and popular name coconut are derieved from Spanish word ‘Coco’ meaning “Monkey face” a probable reference to the three scars on the base of the shell resembling two eyes and a nose of monkey face. Coconut is valued most for its nut, the other components are coconut water, kernel, shell and husk. Coconut water is one of the most refreshing drinks which is also known for its medicinal value. It increases blood circulation in kidneys, causes profuse diuresis and eliminates mineral poisoning. It is a good substitute for saline glucose under gastroenteritis conditions. The coconut has been regarded as Kalpavriksha (tree of heaven) because all the parts of the palm is useful to mankind in one way or the other. The dried kernels commonly called as copra is richest source of vegetable fat i.e. 60 to 67% oil. Coconut is major source of edible oil and it is propagated through seeds. India is largest producer of coir and its product. The coconut plant has about 4-6 years of juvenile phase with 60 years of productive life.

Cashewnut is an important delicious and dollar earning cash crop of India. This crop was remained neglected due to its poor yield potential and prolonged harvesting season. Cashew was introduced mostly for soil conservation purposes. In the beginning of the 20th century cashew kernels gained importance in the international trade as edible nut and the crop began to be considered as having commercial value. Cashew is commercially grown for its kernels although cashewnut shell liquid (CNSL) and apples are also valuable by - products. It is considered to be the most important edible nut in the world trade (Ascenso, 1986). India is the largest producer and exporter of cashewnut in the world. Cashew kernel is the rich source of protein, carbohydrate, unsaturated fats and minerals like calcium, phosphorus and iron. Cashew proteins are complete with all essential amino acids. Fats of cashewnut are considered as complete, attractive, easily digestible and non fattening, it can be used by both olds and infants. The cashew apple juice is rich in vitamin C and it has some medicinal properties.

Pineapple is one of the most important commercial fruits of the world. It is good source of vitamin A, B and fairly rich in vitamin C, minerals like calcium phosphorus and iron. Fresh fruit of pineapple is used as dissert fruit in the form of slices. Fruit is also used for juice, squash, jam, mixed jam and in canned form, fruit cone is used for preparing candy. Some cultivars of pineapple are used for extraction of fibre. The important states of pineapple growing are Assam, West Bengal, Tripura, Kerala, Andhra Pradesh, Bihar, Karnataka and Madhya Pradesh (Bal, 1997).

Bael is one of the oldest and nutritional fruits of India (John and Stevenson, 1979). The tree is very hardy and thrives in almost all types of soil and climate. The ripe fruits of bael are rich in protein, fat, minerals, riboflavin, carotene, thiamine, niacin and vitamin C. The ripe fruit is lucrative while unripe fruit is prescribed for diarrhoea and dysentery (Mitra 1999, Roy, 1996, Singh and Ali, 1992, Singh, 2000). The marmelos in is most probably the therapeutically active principle of bael fruits. 18.1.1 Centre of diversity The Bael tree has its origin from Eastern Ghats and central India. Its indigenous to Indian subcontinent and mainly found in tropical and subtropical regions. Even though it is native to India, it is found throughout Southeast Asia (Neeraj et al., 2017). The tree is also found as a wild tree, in lower ranges of Himalayas up to an elevation of 500 meters. Bael is also found growing along foothills of Himalayas, Uttaranchal, Jharkhand, Madhya Pradesh and the Deccan Plateau and along the east coast (Sharma et al., 2007).

Apple (Malus x domestica Borkh) is the most widely grown temperate fruit of the world considered as king of temperate fruit. It is one of the most delicious fruit rich in carbohydrates, minerals like calcium, phosphorus, iron, potassium, vitamin B6 and low in calories. Apples are extremely rich in important antioxidants, flavanoids, and dietary fiber. Apple is recommended to reduce the incidence of dental caries, and the phytonutrients and antioxidants may help reduce the risk of developing cancer, hypertension, diabetes and heart disease. Apple is chiefly used as table fruit. The various processed product like juices, jelly, preserve, canned slices, wine, concentrated juice, cider and powder are prepared from apple fruit.

Appendix Botanical name, family and origin of important fruit crops