Introduction Veterinary Anatomy is regarded as the firm foundation and the knowledge gained in the subject is the basis for reasoning and helping with the diagnosis and treatment of animals. Teaching gross anatomy is highly regarded as being clinically relevant. The research on a wide range of species in Veterinary anatomy will be helping to be the understanding of their biology. The research conducted helps to understand the anatomy of domestic and wild animals and how this can be applied to diagnostic imaging and other disciplines of Veterinary Science. Hence teaching and research in anatomy provides foundation on which the knowledge of clinical practice and medicine is built. The research in Anatomy pave way for advanced research in other Veterinary Discipline. TEACHING PERSPECTIVES Anatomy education has entered newer and more innovative methods of teaching. “Vesalius” referred to as a founder of modern Anatomy struggled to make dissection of cadavers mandatory in 16th century and its significance is again being questioned today in search of substitutes. Hence use of alternatives to dissected specimens and multimedia teaching methodologies to teach anatomy and basic principles of Veterinary Medicine and Veterinary Surgery have gradually become more popular.
Introduction A deep knowledge of veterinary anatomy is essential for more sustainable livestock management practices, enhancing productivity while minimizing environmental impact. Understanding the physiological structure and function of food-producing animals enables professionals to make informed decisions regarding production, meat processing, and food safety. In today's modern workforce, a comprehensive grasp of animal anatomy is essential for success in various fields, including agriculture, veterinary science, food processing, and quality control. Professionals can enhance their expertise, make data-driven decisions, and drive innovation in the food production sector by grasping animal anatomy. Ultimately, this foundational knowledge is vital for advancing careers and ensuring the highest standards of quality, efficiency, and safety in the industry. Meat processing professionals require in-depth knowledge of animal anatomy to guarantee optimal carcass handling, accurate meat quality evaluation, and streamlined production workflows. Expertise in animal anatomy is also crucial for food safety and quality control specialists, enabling them to ensure regulatory compliance and maintain consumer trust. Additionally, knowledge of animal anatomy allows these specialists to identify potential contaminants and implement effective control measures. By possessing a thorough understanding of animal anatomy, industry professionals can optimize efficiency, quality, and safety throughout the meat processing and distribution chain.
Introduction Adaptations in animals and birds are countless and extensive, which permit the species better suited in its environment and thus improve chances of survival and propagative success. There are three major categories of adaptations namely anatomical/structural, physiological and behavioural adaptations. Depending on the animal habitats, adaptations can be classified into terrestrial, aquatic and volant (flying). Terrestrial adaptations are exhibited by the organisms inhabiting in land habitats and are again grouped into cursorial, fossorial, arboreal and desert adaptations. Terrestrial Adaptations 1. Cursorial Adaptations Cursorial animals are those adapted for running over long distances, often characterized by adaptations that enhance speed and endurance. These adaptations include various skeletal features that optimize their locomotion and overall efficiency while running. These animals have streamlined and spindle-shaped body to minimize resistance in attaining the speed. Here are the key skeletal adaptations in cursorial mammals: 1. Long and Slender Limbs: Cursorial mammals typically have long and slender limbs, which reduce the amount of energy required per stride and increase stride length, thereby enhancing running speed. Lengthening of the limbs in these animals leads to the head placed away from the ground. Hence, as an adaptation to feeding and drinking, the neck and the skull become elongated. 2. Reduced Number of Toes: Many cursorial mammals have reduced the number of toes to streamline the foot and reduce weight. For example, horses typically have a single functional toe (hoof), while cheetahs have reduced and non-retractable claws.
Introduction Lymphatic tissue in general consists of reticulin fibres and fixed cells (macrophages and reticulin cells), as well as free cells (lymphocytes, plasma cells and granulocytes). In loose lymphatic tissue, fixed cells predominate whereas, in dense lymphatic tissue, free cells predominate. The stem cells which colonize the embryonic thymus and bursa originate in the area pellucida or embryo proper in birds. Late in embryonic development, stem cells may be found in the liver and spleen. In adult life they exist primarily in the bone marrow. The avian lymphatic system is divided into: A. Primary lymphatic tissue (central): Primary lymphoid organs in birds include the thymus and the bursa of Fabricius, which are the places where lymphocytes develop, and the T-cell and B-cell receptor genes rearrange. The avian primary lymphoid organs, the thymus and the bursa of Fabricius, undergo age-dependent changes leading in some cases to the complete atrophy of the organ. Interestingly, the bursa of Fabricius can act as both i.e. primary and secondary lymphoid organ. Primary lymphatic tissue is antigen dependant, and in it incoming stem cells differentiate into immunologically competent B and T cells, which then migrate to the secondary lymphatic tissue B. Secondary lymphatic tissue (peripheral): It consists of the spleen, gut-associated lymphoid tissue (GALT), including the caecal tonsil, lymph nodes, bone marrow, mural lymphoid nodules and variable aggregations of lymphocytes throughout the body. Secondary lymphatic tissue is antigen dependent "effector" tissue, in which B and T cells mature into respective effector cells.
In the 19th century, Etienne Geoffroy de Saint Hilaire conducted pioneering studies on prenatal anomalies such as cyclocephaly, anencephaly, and twin monsters, and is credited with coining the term 'teratology'. The physician Josef Warkany is considered the father of experimental teratology. His studies led to the definition of both genetic and environmentally induced structural defects. The occurrence of congenital malformations varies significantly across species, breeds, and geographic locations. Studies suggest that approximately 1.5% to 6% of live-born domestic mammals exhibit recognizable congenital malformations. These malformations range from complex anatomical abnormalities to complicated enzyme defects detectable only through advanced molecular diagnostics. Occurrence of these malformation is very low in cat while in sheep, cattle and horses it is almost 3-4 % and highest in dogs and pigs and is range about 6%. The genesis of congenital malformations is understood as interplay between an embryo's genetic makeup and its developmental environment. Teratology thus highlights the critical periods during embryonic development when external influences can significantly impact normal growth and formation, underscoring the delicate balance between genetic predisposition and environmental factors in shaping fetal development. Teratogens are those substances which may cause inheritable birth defect via a toxic effect on an embryo or foetus. These defects include malformation, disruption, deformation and dysplasia which may cause stunted growth, congenital disorders that lack structural malformation. A fundamental principle of teratology is that the susceptibility to teratogenic agents those causing malformations vary with the embryo's developmental stage at the time of exposure. Exposure during the first three weeks of gestation (stage of preformation), when the basic body plan is established, typically results in early embryonic death or is compensated for by regulatory mechanisms within the embryo. Agents that disrupt the morula or blastocyst stages, or interfere with embryo maternal communication and proper implantation in the uterine mucosa, often lead to early embryonic mortality. The severity of malformation is depends on the frequency and dose of the teratogens. Most of the teratogens affect the embryo during early differentiation and organogenesis stage. It has been found that the organogenesis of urogenital system of bovines, equine, canines, porcine and felines are most susceptible for teratogenic agents.
Introduction Neuroanatomy research covers a wide range of topics including, gross anatomy of brain and spinal cord, imaging technologies for diagnosis, surgical anatomy, microscopic anatomy on nervous system, ultrastructural details on neurons and neuroglia, research on gene and protein expression patterns using in situ hybridization and immunocytochemistry. Despite many challenges, neuroanatomy in animals remains a crucial area of study for understanding the basic principles of structural organisation and its physiology. This review on the master glands i.e. hypothalamus and hypophysis cerebri, is mainly focused on the histomorphology and ultrastructural details reported in rat, rabbit, sheep, goat, buffalo and camel. Mastering the Master gland The hypothalamus (“hypo” meaning below, and “thalamos” meaning nuptial chamber / innermost room) is a major centre of the brain consists of regulatory circuits that controls internal body functions for survival. The hypothalamus is a small, central region of the brain composed of a conglomerate of hypothalamic nuclei and their nerve tracts. The hypothalamic neural connections to the pituitary glands in various mammals have been studied under light microscopic examination of sections of hypothalamus and pituitary gland together intact with infundibular stalk (Paramasivan et al., 2011). The nerve fibres of various hypothalamic nuclei coursed on as a distinct bundle into the infundibular stalk and then continued into the neurohypophysis. Through its connections with pituitary gland, the hypothalamus exerts an enormous influence over the endocrine system and thereby over the general metabolic functions and reproduction (Clemente, 1985). The pituitary gland (hypophysis cerebri) is considered as the master endocrine gland as it plays a critical role in influencing and controlling the activities of other endocrine glands viz. thyroid, adrenal, ovary, testis and pineal. However, the hypophysis itself is under the control of the hypothalamus which actually masters the endocrine function of various hormones released from the master gland i.e. hypophysis cerebri.
A diverse microbial population colonizes the sterile mammalian gastrointestinal tract during and after the birth. This organized and long-lasting process of colonization by microorganisms has developed into flourishing relationships creating diverse environment within the host gut (Mazmanian et. al. 2005). There are various factors that affect the optimum colonization of gut microbiome and their normal functioning on various organ systems of the host body directly or indirectly. The gut microbiome comprises of a complex combination of microbes (Mezouar et. al. 2018). The mammalian gastro-intestinal tract harbors this complex ecosystem in equilibrium with the host immune system. The most magnificent feature of this commensal relationship is that the host not only tolerates but has also evolved to require colonization by commensal microorganisms for its own development and health. There are various factors that affect the optimum colonization of gut microbiome and their normal functioning on various organ systems of the host body directly or indirectly. The process of colonization is a two way interaction between the host and the microorganisms.
Forensic science has traditionally been associated with human criminal investigations, but its application to veterinary cases is gaining momentum. Hair is a specialized outgrowth of epidermis, a component of the skin. Hair is chemically stable especially when compared to other physiological materials such as blood, semen or any other f luid as it is strongly resistant to decomposition and this property makes hair a nearly ideal type for physical evidence. However, the forensic identification of animal hair requires different skill sets and competencies to those required for human hair comparisons (Tridico et al. 2014). Hair carry a lot of biological information, their examination is easy and very cost effective. Recently Funes et al. (2023) has reviewed both macro and micro quantitative methods for human hair analysis. The study of the animal hair could represent a useful tool in veterinary forensic investigation and thus can be helpful in species identification in veterolegal cases, illegal transport of slaughtered animals, poaching or wildlife crime, fraud in textile and fur industry, traceability of predators and identification of their victims (Dahiya and Yadav 2013). The use of hair analysis in forensic science dates back to the 19th century, with early forensic scientists recognizing its potential in criminal investigations. Over time, techniques have evolved from simple visual comparisons to sophisticated microscopic and biochemical analyses.
Research techniques are the various methods and tactics strategically used to collect the materials, record the observations and analyse the data for study. These approaches provide a structured framework for achieving research objectives and validate the findings. Anatomy is the oldest scientific discipline of the medicine, which deals with the form and structure of the organisms. The first documented scientific dissection on the human body were carried out as early as in the third century BC in Alexandria. At that time anatomists explored anatomy through dissection of animals, primarily on pigs and monkeys. The first written evidence of anatomical terms and rational observations can be found in the Egyptian medical papyri. The work of Andreas Vesalius played a significant role in medical research and education. During the 19th century the research in anatomy increased due to discovery of microscopes which allowed in-depth studies of body tissues and their components. However, in the last 100 years due to technological innovations and the growing understanding of related sciences progress in anatomy took place at a much faster pace. A thorough knowledge of research techniques in anatomy is very important to perform systemic experiments to reach a definite conclusion. The technique should be selected according to the aim and field of research. Here we are going to discuss some of the important gross anatomical techniques.
The escalating global demand for livestock products, driven by rapid population growth and rising per capita consumption, presents significant challenges for the agricultural sector (Moreau and Jordan, 2005). Traditional livestock production methods are becoming inadequate to meet this demand sustainably. Biotechnology offers novel solutions to enhance productivity, improve animal health, and ensure environmental sustainability (Schmitt and Henderson, 2005). This review aims to explore the role of biotechnology in livestock, focusing on key innovations and their implications for the future of animal agriculture. 1. Genetic Engineering Genetic engineering has revolutionized the field of animal science by enabling precise modifications of the livestock genome to enhance desirable traits and reduce undesirable ones. 1.1 Transgenic Technology A mouse was the first successful transgenic animal followed by pigs, sheep, cattle, and rabbits (Shakweer et al., 2023). Transgenic animals are created by inserting foreign genes that encode specific traits, such as growth hormone genes to enhance growth rates or disease resistance genes to reduce susceptibility to infections (Hammer et al., 1985). For instance, transgenic cattle have been engineered to produce milk with modified protein content (Zhang et al., 2019), improving its nutritional profile for human consumption (Brophy et al., 2003). Further, cattle genetically engineered to express human lysozyme in their milk have shown enhanced antimicrobial properties, making the milk safer for consumption (Moreau and Jordan, 2005). These modifications not only boost productivity but also improve the quality of livestock products, making them healthier and more nutritious.
Veterinary anatomy is a cornerstone of veterinary education, essential for diagnosing and treating animals. It imparts knowledge about origin, form, structure and function in animals. Traditionally, veterinary anatomy education has been delivered through lectures, dissections, and practical sessions. Anatomy teaching has undergone significant changes to keep up with advances in technology and to cater for a wide array of student specific learning approaches. In recent time a generalized decline in dissection based veterinary anatomy teaching has been observed due to implementation of new curriculum of veterinary anatomy by VCI, legal and ethical issues, unavailability of ethically sourced cadaver, reduced teaching hour to veterinary anatomy, reluctance of students as well as teacher to handle formalin fixed cadavers and specimens etc. Even with the introduction of modern instructional technology and improved teaching methods, dissection continues to remain a foundation of veterinary anatomy teaching. Conventional methods, while foundational, face significant limitations, including the availability of resources and variability in hands-on experiences. As veterinary education evolves, there is a growing recognition of the potential for disruptive technologies to address these challenges and enhance learning outcomes.
As we delve into the subcellular world, we uncover pivotal insights for advancing our understanding of animal health and disease. Immunoelectron microscopy (IEM) is the same as immunohistochemistry or immunocytochem istry at the light microscopy level. The Power of IEM combines the high-resolution imaging capabilities of electron microscopy with the specificity of immunolabeling. This robust procedure allows us to visualize the precise local ization of proteins and other molecules within cells, providing a detailed cellular architecture and function map. This technique is known as immunogold staining (IGS) or immunogold labelling (De Paul et al., 2012). Principle of Immunoelectron Microscopy (Iem) The basic principle of IEM is based on the specific binding of a primary antibody to the target antigen in the sample. A secondary antibody, which is conjugated to colloidal gold particles, is then added. This secondary antibody binds to the primary antibody. The gold particles are electron-dense, meaning they scatter electrons effectively. If viewed under an electron microscope, these particles appear as dark spots, indicating the location of the target antigen. This method demonstrates very specificity and high contrast, making it easier to visualize and study the distribution of proteins at the ultrastructural level (Schwartzbach & Osafune, 2010; Murtey, 2016).
Introduction Equines are a fascinating and diverse group of mammals that have played a significant role in human history and continue to do so today. From the majestic horses used for transportation, sport, and work, to the hardy donkeys and mules used for farming and packing, equines have been an essential part of human societies for thousands of years. Equines are social animals and usually live in groups or herds. They form strong bonds with their herd members and communicate with each other through a range of vocalizations, body language, and scent cues. Equines also engage in playful behaviour, particularly when they are young. They can be seen running, kicking, bucking, and frolicking in the fields as a way of expending energy and developing physical coordination [6]. This is a group of mammals that belong to the family Equidae known for their slender legs, hooves, and long faces. Large, soft and expressive eyes, a velvety muzzle, a forelock adorning a smooth face these are just a few of the attributes of the equine head that beckon horse lovers. The equine head, which accounts for 10% of a horse’s total weight, houses a series of intricate turbinates within the nasal passageways. Turbinates are a network of bones, vessels, and tissue that warms, humidifies, and filters the air the horse inhales. In addition, seven air-filled sinus cavities called the paranasal sinuses sit on each side of the head, above, below, and between the eyes. A young horse starts out with teeth that extend up into the sinuses; with wear and age, the teeth descend into the mouth. Eventually, in a horse’s golden years, the sinuses are mostly empty of teeth.The head also contains a plexus of nerves and blood supply in addition to the eyes, ears, nostrils, lymph nodes, guttural pouches, brain, and more than 30 bones[6,7].
The present study was carried out on gross anatomical and morphometrical studies of extensor and flexor muscles of digits of forelimb and hind limb in six adults non-descript dogs irrespective of sex. Common digital extensor muscle was originated from the lateral (extensor) epicondyle of the humerus with multiple tendons of insertion whereas lateral digital extensor was originated from the lateral ligament of the elbow joint. Extensor pollicis longus and indicisproprius muscle was present under the lateral digital extensor and superficial to the abductor pollicis longus and originated from the middle third of the dorsolateral border of the ulna. Deep digital flexor muscle was comprised of two heads, flexor hallucis longus, and flexor digitorum longus.The average tensile strength for common digital extensor, lateral digital extensor, extensor pollicis longus and indicisproprius, and abductor pollicis longus muscles were 2.65 ± 0.36 kg, 2.20 ± 0.47 kg, 1.60 ± 0.38 kg and 1.95 ± 0.35 kg respectively. Mean Muscle belly length for common digital extensor, lateral digital extensor, extensor pollicis longus and indicisproprius, and abductor pollicis longus muscles was 8.30 ± 0.51 cm, 6.68 ± 0.45 cm, 4.80 ± 0.68 cm and 8.08 ± 0.73cm respectively. Mean tensile rest strength for the superficial digital flexor, deep digital flexor, interosseous muscle, f lexor pollicis brevis and lumbricales was 7.61 ± 0.56 cm, 8.53 ± 0.51 cm, 2.76 ± 0.47 cm, 0.73 ± 0.13 cm and 0.85 ± 0.17 cm respectively. Mean muscle belly volume of superficial digital flexor, deep digital flexor, interosseous muscle, flexor pollicis brevis, and lumbricales muscles was 6.61 ± 0.72 cm3, 7.84 ± 0.53 cm3, 0.24 ± 0.07 cm3, 0.03 ± 0.01 cm3, 0.02 ± 0.00 cm3 respectively.Mean tendon cross section area for muscles tibialiscranialis, long digital extensor, lateral digital extensor, and extensor digitorum brevis was 0.05 ± 0.00 cm2, 0.06 ± 0.00 cm2, 0.04 ± 0.00 cm2 and 0.04 ± 0.00 cm2 respectively.
