Any animal husbandry system’s production is highly sensitive to the health of the herd. For farmers and livestock in general, deviations from the maintenance of optimal herd health can have serious repercussions. Infectious diseases in animals and birds, both emerging and endemic, are a severe economic loss for our nation’s distinctive sector. Disease diagnosis is a requirement for both containing and controlling such outbreaks.
Any animal husbandry system’s production is highly sensitive to the health of the herd. For farmers and livestock in general, deviations from the maintenance of optimal herd health can have serious repercussions. Infectious diseases in animals and birds, both emerging and endemic, are a severe economic loss for our nation’s distinctive sector. Disease diagnosis is a requirement for both containing and controlling such outbreaks.
Collection of Blood Objective: To diagnose the existing disease condition, if any. Types of blood collection • Arterial sampling: In order to measure arterial blood gases, an arterial blood sample is taken from an artery. Only veterinary professionals who are legally permitted to do so for their work in their country and who have proven their expertise via formal training should take arterial blood samples. • Venipuncture sampling: The most used method of taking blood from humans and animals is venipuncture. Collection occurs from a superficial vein near the surface that is not very close to any large nerves.
Clinical Significance The primary aim of laboratory diagnosis is to assess the carbohydrate metabolism impairment in diabetes mellitus, inducing the following: 1. Determination of glucose concentration in fasting blood and demonstration of postprandial glycosuria. 2. Assessment of glucose tolerance
The liver produces the entire albumin and most of the globulin; a small amount of gamma globulin is produced by retculoendothelial tissue. The production of albumin is subject to a mechanism regulating colloidal osmotic pressure and the secondary to alterations in globulin concentration. The liver synthesizes lipoproteins, glycoproteins, mucoproteins, fibrinogen, prothrombin and clotting factors VII, VIII, IX and X.
A. Triglycerides Clinical significance To form lipoproteins, triglycerides combine with cholesterol, phospholipids, and plasma proteins. To determine whether hypertriglyceridemia may be present, the serum triglyceride concentration is measured (increased blood and serum levels of triglycerides). Chylomicrons and very low density lipoprotein (VLDLs), which cause the extensive lipaemia frequently observed in serum or plasma samples, are both mostly composed of glycerides.
Calcium Clinical Significance Calcium is an essential mineral which is involved in many body systems. These include the skeleton, enzyme activation, muscle metabolism, blood coagulation and osmoregulation. In the blood calcium exists as 50% ionized, 40% protein bound and 10% complexes with anions such as citrate and phosphate. Only ionized calcium is biologically active in bone formation, neuromuscular activity, cellular biochemical processes and blood coagulation. Factors governing the total plasma concentration are complex and include interaction with other chemical moieties, proteins and hormones. Calcium, phosphorus and albumin metabolism are interdependent.
Sodium Clinical Significance Compared to potassium, sodium is distributed differently throughout the body. Due to the sodium pump mechanism, most sodium is extracellular. Compared to this, just 2% of potassium is extracellular; the remainder is intracellular. Regarding the plasma concentrations of sodium and potassium, renal function is the single most significant homeostatic mechanism.
Creatinine Clinical significance Creatinine is produced at a steady rate due to muscle catabolism and is not reabsorbed by the kidney tubules after filtration. Its measurement provides an indirect assessment of the glomerular filtration rate (GFR). Unlike urea it is not markedly affected by diet or any aspect of liver function and therefore the test is more specific for renal dysfunction
Urinnalysis An essential laboratory test that is easily conducted in veterinary settings and is included in a basic database is urinalysis. Documenting diverse urogenital tract conditions is helpful, because it could reveal details about other systemic conditions including hemolysis and liver failure. Cystocentesis, urethral catheterization, or simply voiding can all be used to collect urine. Urine should be examined within 30 minutes, but if this is not possible, it can be refrigerated for up to 24 hours or sent to a outside diagnostic lab; this, however, may cause crystal precipitation. The pH or specific gravity of urine are unaffected by refrigeration. Preservative should be added if cytologic analysis is necessary and a delay in slide preparation is anticipated. A few drops of 10% formalin (exact concentration not necessary) are better than boric acid
Collections of blood To collect blood for different haematological analyses, an animal’s vein are punctured with a syringe and needle. Requirements • Hypodermic needle • Syringe • Collection vials or tubes containing anticoagulantsProcedure • The place of blood collection should always be cleaned with alcohol and hairs trimmed. • Press on the vein to raise it. • Free-flowing blood can be collected directly into the vial containing anticoagulant after the needle is inserted into the vein. • 5 ml of blood from large animals and 2.0 ml of blood from small animals like dogs and cats are sufficient for normal haematological investigations. • Rotational motions in the vials are used to mix the blood
Purpose of study An essential component of the diagnostic process in the evaluation of the disease process is the laboratory diagnosis of any parasitic infection. Sometimes, this confirms the presumptive clinical diagnosis, and other times, it provides evidence that the clinician may have ignored. A sufficient specimen must be used for the examination to achieve a satisfactory diagnosis. Internal parasite testing needs to be done often. Regular inspection typically enables early parasitism identification, preventing gross infection. The majority of the parasites can be accurately identified by doing a detailed examination of the faeces. Faecal sample collection and delivery for parasite identificatio
A. Intestinal protozoa Fresh faeces must be used as a sample. The sample must be examined immediately and without refrigeration. Methods of examination 1. Direct smear examination A thin smear of faeces mixed with either water or normal saline (recommended for Trophozoites since they stay intact and motile longer). Select blood-tinged mucus or tiny tissue bits if the faeces are dysenteric. One to two percent of an aqueous eosin solution can be used in place of saline or water, but the oocyst still resist the stain. The viability is examined using the eosin solution. It is possible to employ an iodine solution (which kills protozoa), which allows for the identification and differentiation of the protozoa. It is possible
The essential database for all skin diseases consists skin scrapings. Skin scrapings fall into two main categories: superficial and deep. Superficial scrapings give information from the epidermis’s surface without causing capillary bleeding. Capillary haemorrhage is a sign that the sampling was deep enough to gather material from the hair follicle. Deep skin scrapings are used for this purpose. The main purpose of skin scrapings is to detect the presence or absence of mites. A skin-scraping spatula, which is a thin metal weighing spatula, is recommended for use while scraping the skin. These spatulas are safe and can be used again. Scrapings from active lesions at several places are to be submitted. Scraping from active lesions at several places is to be submitted. Generally, the periphery of the lesion contains more parasites than the centre. Skin scrapings are used to identify ectoparasites and fungal diseases.
Reagent/solution 1. RBC diluting fluid a) Gower’s solution Sodium sulphate Glacial acetic acid Distill water b) Hayem’s fluid Sodium sulphate Sodium chloride Mercuric chloride Distill water 2. WBC diluting fluid a) For animals Glacial acetic acid Distill water 2.5 gm 16.6 ml 100.0 ml 2.5 gm 0.5 gm 0.5 gm 100.0 ml 3.0 ml 97.0 ml
