eChapters

Chromosomes are giant molecules of DNA

Found in nucleus: never leave except in cell division

Carry hereditary units, genes

Two homologous copies in diplod cells

Unwind when replicating or making RNA

Wind up (condense) in cell division

Attach to spindle and separate in meiosis & mitosis

Crossing over in prophase of 1st meiotic division

Chromosomes are equally distributed in mitosis, meiosis

Gametes have half the number of chromosomes as body cells

Sister chromatids are observed to split apart longitudinally in anaphase

UNTIL THE 1940S BIOLOGISTS ARGUED ABOUT WHETHER DNA OR PROTEIN WAS THE MOLECULE OF HEREDITY

Nuclei observed to divide when cells reproduced themselves

Large amounts of both DNA & protein in the nucleus

Many though protein was most likely molecule of heredity

Proteins made of 20 different amino acids- could have more variety than DNA (made of only 4 different bases)

Restriction enzymes cut only at specific sequences of 4 to 8 bases

A long restriction site is rare. Cutting rare sites will produce a few large pieces of DNA.

Restriction enzymes with long sites → small number of large fragments

Restriction enzymes with short sites → large number of small fragments

INTRODUCTION

Prediction of helminthic infections is impractical. The aim of any control programme must therefore, ensure that parasite populations do not exceed levels compatible with economic production. The parasitological monitoring (e. g. faecal egg counts or pasture sampling) at intervals, forecasting on the basis of meteorological data and computer simulation may provide an alternate approach to control parasitic infection (Brunsdon, R. V. , 1980) in a given geographical area. The most common gastrointestinal parasite prevalent throughout the year in Meghalaya, India is Strongyle infection. This is because of the high rainfall and humidity prevalent in the North-Eastern region.

As the prevalence of this infection is mainly dependent on rainfall and humidity, study was being initiated to identify the relationship between rainfall/temperature and strongyle infection. The study would be also helpful in predicting/ forecasting the disease occurrence based on the prevailing pattern of rainfall in a particular location.

Principle

The principle is to extract plasmid DNA from bacterial cell suspensions and is based on the alkaline lysis procedure developed by Birnboim and Doly (Nucleic Acids Research 7:1513, 1979). It is a known fact that plasmids are much smaller super coiled DNA molecules than the much larger and less super coiled bacterial chromosomal DNA. This difference in topology enables selective precipitation of the chromosomal DNA and cellular proteins and separates them from plasmids and RNA molecules. The cells are lysed under alkaline conditions, which denature both nucleic acids and proteins, and when the solution is neutralized by the addition of Potassium Acetate, chromosomal DNA and proteins precipitate because it is impossible for them to renature correctly (they are so large). Plasmids renature correctly and stay in solution, effectively separating them from chromosomal DNA and proteins.

Principle

The rate of joining of DNA fragments is affected by seven parameters:

DNA concentration,

Concentration of compatible ends,

Size of the fragments,

Presence or absence of complementary, single-stranded ends (some nucleases form blunt ends and in these cases special techniques are needed for joining),

G + C content and length of the complementary single strands,

Temperature of the reaction and

Ionic composition of the solution.

Steps for Cloning

Prepare DNA.

Select restriction enzymes for your insert and vector, and determine the appropriate reaction buffers.

Combine the following in a microfuge tube (30 µL total volume):
2 µg DNA
1 µL Each Restriction Enzyme
3 µL 10x Buffer
3 µL 10x BSA
x µL H₂O (to bring total volume to 30 µL)

Principle

To retrieve and purify any specific DNA fragment from an agarose gel slice; the expected yield is from 50-75% of the amount in the gel slice.

Time required

Restriction digest - minimum of 2 hours

Electrophoresis - approx. 4 hours

Elution - 2-5 hours depending on fragment size

DNA Purification -2 hours

Principle

RNA is negatively charged at neutral pH and when an electric field is applied, it migrates towards the anode. RNA retains much of its secondary structure during electrophoresis unless it is first denatured. The addition of formaldehyde to the agarose gel maintains the RNA in its linear (denatured) form. The following protocol is based on Formaldehyde Agarose Gel Method.

This protocol is designed for potato stem internodes on ZIG medium, but might work for leaf pieces as well.

Plant Material

Cut stem internode explants from 5-6-week-old in vitro plants (or leaf explants from 3-4-week-old in vitro plants) grown on 15 ml PROP in 25 x 150 mm test tubes.

Day 1

Streak out desired Agrobacterium strain on YEP + antibiotic to obtain a single colony. Grow at 28 C for 2 days. Alternatively, skip this step and use frozen stock for next step.

Day 3

Use a single Agrobacterium colony to inoculate 20 ml YEP + antibiotic in 250-ml flask. Grow overnight at 28 C with vigorous shaking (~200 rpm). Some Agrobacterium strains require an additional day to reach sufficient density.

Information is a basic resource essential for individual and organization in order to survive and succeed in society. Floriculture is a technology driven industry needing technological advancement. The new technologies that are being developed at various places the world over, needs to be disseminated through proper and quick linkage between the researchers and the beneficiaries i.e. the farmers. This demand can be met out by information technology. Information is data put into a meaningful and useful text. Today, information technology (IT) is a metaresource and computers are being used as a metatool and a metamedia.

COMPONENTS OF INFORMATION TECHNOLOGY

An information technology architecture is an integrated framework for acquiring and evolving IT to achieve strategic goals. It has both logical and technical components. Logical components include mission, functional and information requirements, system configurations and information flows. Technical components include IT standards and rules that will be used to implement the logical architecture.

Input devices: The advent of touch screen monitors has made it possible to develop user-friendly interface for farmers. It is now possible to capture and store good quality graphics and video clips with digital cameras.

Output devices: Output devices like monitor screen, printers and plotters, data projectors support high resolution and good quality output.

Processors: The processing speeds of computers have improved a lot these days. At present, Intel P-IV based processors @ 3.3 Ghz are available in the PC range.

Storage devices: To store substantial information at local level. Capacity of 40 GB and higher are common in hard disk devices, besides floppy disk drives and CDs; all making it possible to transfer large amount of data to locations which cannot be connected to networks quickly.

World Trade Organization (WTO) is an international body which deals with the “Rules of Trade” between nations on the basis of the agreements negotiated and signed by most of the world’s trading nations. These agreements are essentially contracts that bind governments to keep their trade policies within predefined limits. The goal is to help producers of goods and services, exporters and importers.

The purpose of WTO agreement is to help free flow of trade, serve as a forum for trade negotiations and to serve as a dispute settlement body. There are 5 broad principles of the trading system followed under WTO:

Trade without discrimination

Freer trade, gradually through negotiations

Predictability, through binding

Promotion of fair competition

Encouragement to development and economic reforms.

CONTAMINATION

The healthy inner flesh of meat contains few or no microorganisms, although they have been found in lymph nodes, bone marrow and intestine. Staphylococcus, Streptococcus, Clostridium and Salmonella have been isolated from lymph nodes of red meat animals. During slaughtering the lymph nodes are removed from edible parts. The important contamination, however comes from external sources during bleeding, handling and processing. During bleeding, skinning and cutting the main sources of microorganisms are from the exterior of the animal- hide, hoof and hair and the intestinal tract. The exterior of the animal harbours large number and many kinds of microorganisms from soil, water, feed and manure as well as its natural surface flora.

Knives, cloths, air and hands and clothing of workers can serve as intermediate sources of contamination. During handling and processing the contamination can come from containers, air, other contaminated meat, equipments and personnel.

Molds that grow on the surface of the meat include the species of Cladosporium, Sporotrichum, Geotrichum, Mucor, Monilia, Penicillium and Alternaria. The important bacteria found in meat are Staphylococcus, Sarcina, Streptococcus, Clostridium, Leuconostoc, Lactobacillus, Proteus, Flavobacterium, Bacillus, Streptomyces, Escherichia and Salmonella.

14.1. EGGS

India is the third largest producer of eggs. Fresh eggs are gathered on automatic collection belts at the farm and stored in a cooler at about 7°C. The eggs are then delivered to a central processing plant, where they are washed, sanitized, and graded. Grading involves the sorting of eggs into size and quality categories using automated machines. Flash candling (passing the eggs over a strong light source) detects any abnormalities such as cracked eggs and eggs containing bloodspots or other defects. Higher-grade eggs have a thick, upstanding white, an oval yolk, and a clean, smooth, unbroken shell.

14.1.1. Eggs Contamination

More than 90 percent of all eggs are free of contamination at the time they are laid; contamination with Salmonella bacteria and with certain spoilage organisms occurs essentially afterward. They become contaminated by faecal matter from the hen, by the lining of nest, by handling, by wash water and packing materials. The total number of microorganisms per shell of a hen’s egg was found to range from 100 to 10,000,000 with a mean of about 100,000. Proper washing and sanitizing of eggs eliminates most Salmonella and spoilage organisms deposited on the shell. The organism Salmonella enteritidis, a common cause of gastroenteritis (a form of food poisoning), has been found to be transferred through the hen ovary in fewer than 1 percent of all eggs produced. Ovarian- transferred S. enteritidis can be controlled by thorough cooking of eggs (i.e., until there are no runny whites or yolk).

Bacterial species vary greatly in the ability of their cultures to remain alive after the completion of growth. Some species such as Neisseria gonorrhoeae and Streptobacillus are poorly viable and their cultures usually die out within a few day, whether kept at 37°C at room temperature or at 4-5°C; thus, they must be sub cultivated every 2-4 days for maintenance in the laboratory. Other species are much hardier,. especially sporing species which may remain viable for many years. There are also many non - sporing species (enterobacteriaceae) whose cultures, under suitable conditions, commonly remain viable for many months and often for as much as 10-20 years.

This chapter sheds light on a geohazard namely quicksand. Unlike other geohazards, it waits for its victims to cause hazard. Though discounted as a myth and also as a monster problem in Hollywood film stories, phenomenon of quicksand is real and could inflict life threatening dangers. Analyses of genetic causes, associated properties and locales of its occurrences indicate that more deaths might have resulted due to panic and drowning in waters than by quicksand itself. Based on these inferences, potential mitigation measures are presented in this chapter.

INTRODUCTION

Quicksand, referred as “pudhai manal” in Tamil, is one of the lesser known dangerous natural phenomena that cause death of human and other unsuspecting animals. It is a geohazard of different sort. It is different in a sense that it never “goes” anywhere like tsunami or causes hazard like flood or landslide or any other geohazard; but it waits for hapless victims; that is., it is harmless until human beings or other fauna step into the region of quicksand. It is commonly prevalent in many river beds and other locales near water bodies located amidst dense forests. Many a deaths occur due to quicksand but go unnoticed owing to its “non catastrophic” nature. It is also a geohazard less understood pertaining to causes of formation, potential to cause damage and occurrence (www.mythbuster.com). This chapter is an attempt to review the existing literature on this geohazard for better understanding.

Abstract

The continental drifting and the movement of Indian plate to the tune of 6500 km in a time frame of 65 million years towards north north-easterly, its collision with Eurasian plate, the rise of mighty Himalayan mountains, the still prevalent northerly directed compressive force which has originally drifted the Indian plate and the disseminated intraplate seismicities have been the proven facts. In this context, a remote sensing based study carried out using IRS satellite images revealed that the whole Indian plate is whirling like a worm with series of alternately arranged E-W trending arches and deeps and N-S extensional/block faulting, NE-SW & NW-SE strike slip faults and E-W trending fracture swarms from Cape Comorin in the South to foot hills of Himalayas in the north. These ongoing tectonic activities have displayed hierarchy of geomorphic anomalies in the fluvial, coastal and hydrological systems and these suggest that the whole Indian subcontinent warrant detailed studies to understand the post drift kinematics and its impacts over the ecosystems, natural resources and natural disasters.

Introduction

Consequent to the continental drifting, the Indian plate has moved towards north northeasterly and collided with Eurasian plate, thus resulting into the rise of Mighty Himalayan Mountains along the junction of these two plate boundaries as demonstrated by many earlier Geoscientists. But the behaviour of the Indian plate, both during the collision and after that, has not been comprehensively studied and understood as to whether it has remained as an inert plate or suffered any mid plate deformations. However, disseminated information has been brought out by many earlier workers on the post trappean arches, deeps, horsts and grabens within the

Indian plate. Some of such tectonic grains from north to south in the Indian Peninsular are:

Factor cost : A measure of output reflecting the costs of the factors of production used, rather than market prices, which may differ because of indirect tax and subsidy.

Factors of production : The ingredients of economic activity : land, capital, labour and enterprise.

Factory prices : The prices charged by producers to wholesalers and retailers. Because these prices are eventually passed on to the end customer, changes in factory prices, also known as producer prices, can be a leading indicator of consumer price inflation.

Fair trade : Many politicians and NGOs argue that free trade is not enough; it should also be fair. On the face of it, fairness is self-evidently a good thing. However, fairness, in trade as in beauty, lies in the eye of the beholder. Frederic Bastiat, a 19th-century French satirist, once observed that the sun offered unfair competition to candle makers. If windows could be boarded up during the day, he argued, more jobs could be created making candles. American trade unions complain that Mexicans’ lower wages, say, give them an unfair advantage. Mexicans say they cannot compete fairly against more productive American counterparts. Both sides are wrong. Mexicans are paid less than Americans largely because they are, in general, less productive. There is nothing unfair about that; indeed, it helps to make trade mutually beneficial. The mutual benefits of trade also disprove the fair traders’ other complaint, that free trade harms poor countries. (See comparative advantage).

FDI : See Foreign direct investment.

Fee : A charge for a service rendered.

Observational study : Epidemiological study in situations where nature is allowed to take its course. Changes or differences in one characteristic are studied in relation to changes or differences in others, without the intervention of the investigator.

Odds ratio : A measure of association which quantifies the relationship between an exposure and health outcome from a comparative study; also known as the cross-product ratio.

OECD : The Organisation for Economic Co-operation and Development, a Paris-based club for industrialised countries and the best of the rest. It was formed in 1961, building on the Organisation for European Economic Co-operation (OEEC), which had been established under the marshall plan. By 2003, its membership had risen to 30countries, from an original 20. Together, oecd countries produce two-thirds of the world’s goods and services. The oecd provides a policy talking shop for governments. It produces forests-worth of documents discussing public policy ideas, as well as detailed empirical analysis. It also publishes reports on the economic performance of individual countries, which usually contain lots of valuable information even if they are rarely very critical of the policies implemented by a member government.

Table shell : A table that is complete except for the data.

Table : A set of data arranged in rows and columns.

Tangible assets : Assets you can touch : buildings, machinery, gold, works of art, and so on. Contrast with intangible assets.

Tariff : Often used to describe a tax on goods produced abroad imposed by the government of the country to which they are exported. Many countries have reduced such tariffs as part of the process of freeing up world trade.

Tax arbitrage : Creating financial instruments or transactions that allow the parties involved to exploit loopholes in or differences between their tax exposures, so that all involved pay less tax.

Tax avoidance : Doing everything possible within the law to reduce your tax bill. Learned Hand, an American judge, once said : “There is nothing sinister in so arranging one’s affairs as to keep taxes as low as possible … nobody owes any public duty to pay more than the law demands.” Contrast with taxevasion.

Tax base : The thing or amount to which a tax rate applies. To collect income tax, for example, you need a meaningful definition of income. Definitions of the tax base can vary enormously, over time and among countries, especially when tax breaks are taken into account. As a result, a country with a comparatively high tax rate may not have a high tax burden if it has a more narrowly defined tax base than other countries. In recent years, the political unpopularity of high tax rates has lead many governments to lower rates and at the same time broaden the tax base, often leaving the tax burden unchanged.

All scientists working with laboratory animals must have a deep ethical consideration for the animals they are dealing with. From the ethical point of view it is important that such considerations are taken care at the individual level, at Institutional level and finally at the national level.

Individually each investigator has an obligation to abide by all the ethical guidelines laid down in this regard at institutional level. The Head of the institution, maintaining animals for scientific experiments , should constitute an animal ethics committee on animal experimentation to ensure that all experiments conducted on animals are rational , do not cause undue pain or suffering to the animals and only minimum number of animals are used. The constitution and term of reference of the animal ethic committee should be well defined.

An animal ethic committee should include: a senior biological scientist of the institute, two scientists from different biological disciplines, a veterinarian involved in care of animals, the scientist in charge of animal facility, a scientist from outside the institute & a nonscientific socially aware member. A specialist may be co-opted while reviewing special projects using hazardous agents such as radioactive substance and deadly micro-organisms etc. The investigator may be called in for any clarification if required.

The ethics committee has to examine all projects involving use of animals before implementation, to ensure that minimum number of animals is used in the project and the ethical guidelines are strictly adhered to. It will also examine that the scientists and technicians handling animals possess adequate skill to perform the experiment. All animals should be maintained under standard living conditions and experiments will be conducted under proper anesthesia and termination of an experiment, the animal will be humanely sacrificed under anesthesia. Before disposal it must be ensured that the animal is clinically dead.

Rabbits: Housed in mesh floor cages made of galvanized iron, aluminium or steel.

Cleaning should be done regularly (but not within the animal room). Clean feed, water should be given to animals to keep them free of diseases.

Guinea pig: In case of guinea pig, environmental factors play a very important role → Temperature, relative humidity, lighting and ventilation.

Temp → 18-21°C for all laboratory animals

Relative Humidity → 45-55%

Lighting → 12-16 hours for normal physiological activity.

Ventilation → Min 12-15 changes of air for animal room. This can be provided with the help of exhaust fan.

For guinea pig, temperature should not go beyond 30°C, because they cannot dissipate heat from body and they suffer from heat stress and heat frustration because they have loose fold of skin. Highly susceptible for respiratory infection, so adequate ventilation should be given.

They can be housed on wire mesh, floor, and cages. Also directly on floor provided with bedding material → paddy/ ragi straw. In some animal facilities they give saw dust as bedding material. For Guinea Pig saw dust should be given as bedding material as they get adhered to vulva that causes irritation and also it blocks the vulva. There will be reduction in fecundity of the animal →worst

Mice are omnivores and can be fed with commercial feeds like pellets or fed with mixed feed which is locally available cereal, pulses and grains.

However, nutritionally requirement of mice

Carbohydrates - 45-55%

Protein - 16- 24%

Fibre - 4-5%

Fat - 4-6%

Supplement vitamin and minerals.

Extension worker play a important role in rabbit keeping. It increases food production and experience in rabbit farming, people has to be educated as they have no knowledge of rabbit keeping especially people of towns and villages.

These have to be told the advantages of rabbitry. It is difficult to convince villagers, so extension is very important.

Rabbit Census : Recently taken up.

In Karnataka, census was taken in 1990. There were 16,327 animals.

Buck can be mated to several does not gives many young ones. So quality of buck will be passed on to many number of young one. Choose bucks whose relatives have good commercial character performance can be known by looking at breeding record should have too well development testicle, have good growth, good healthy condition, not be too fat, good physical condition.

Best time is when animal is 2 kgs of body weight and age of 3-4 months.

It is advisable to purchase buck from good reliable source since it brings new characteristics and avoid inbreeding.

The mating of the buck of particular breed with die of another breed is cross breeding usually cross breeding has better commercial value → Hybrids which has hybrid vigour → grow faster, better litter size.

Usually breeding of 2 hybrid is not advisable because we will not be sure which hybrid characteristics will be passed on to young ones.

The back yard rabbitry has mixed breeding programme of low quality with reference to commercial characteristics. To improve back yard rabbitry, good well known breeding buck should be introduced to have better performance.

HOUSING

Dogs are kept in cages and they are tied with a chain in one corner of the house or left free in the house → but hair falling will be seen throughout → allergy.

House in wire mesh all round and wooden flooring → gives warmth to animal. Roof can be made of asbestos sheet.

Cage → 2 ft LX 3 ft BX4 ft H. This should be kept in a cool place.

With cement covering all round with mesh door asbestos roofing.

Dog is always chained. This should be long enough and strong enough to withstand pulling of animal. Along with this collar is provided with strong buckles.

Docile animals can be left free in the house → Pomerian, Dash hound, Dobberman should be chained.

Petting too much leads to transmission of diseases → parasite and allergy.

CATS

Cats are classified under family Felidae. Felidae is divided into 3 sub families.

False sabes (Toothed cats)

Felids (Cats → Felinae)

Big felids (Pantherine)

Felinae

28 species, have been classified in 15 genera.

Chromosome no 2n=38.

Exterior Morphology of Cats

IDENTIFICATION OF ANIMALS

It has been in practice since long, not recent item in livestock farming. In earlier day’s in Egyptian era, people used to mark the animal to identify their animals. But in recent days, due to development in bio-medical science where usage of animal has increased within turn has become necessary to establish a suitable identification method for various species of animal. There are many methods available to identified animal, however the methods employed should be simple, economical and easy to identify animal and should be harmless to animal, quickly applicable and should be suitable to experiment conducted.

COMPOSITION OF DIET

Objectives

• To study the general considerations on feeding aspects of laboratory animals.
• To get acquainted with the specific nutritional requirements of laboratory manures.
• To familiarize with the watering system.
• To know the daily feed schedule of different species of laboratory animal.

Materials Required

Laboratory animals, feed ingredients, water, green vegetables.

For having good growth and production, proper balanced diet should be fed, thus nutrients can be classified into 6 classes:

Objectives

• To keep the laboratory animal house neat and clean
• To maintain healthy stock

The disease may be bacterial, viral, fungal or of parasitic origin. All the measures taken to reduce growth and also to control spread of pathogenic organism is a sanitary measure like ventilation, building without cracks crevices.

Important aspect of good sanitation is insect control or pest control. Most effective measure is to just chase them out. To achieve this :

Committee recognized throughout world on laboratory animal called International Committee on Laboratory Animal (ICLA) This committee is responsible to make the rules and regulations and laws which governs production, maintenance and usage of laboratory animal. This committee has defined Specific Pathogen Free (SPF) animal which are free of specified micro organisms and parasites but not necessarily free of the ones which are not specified. Example pasteurella free SPF animal.

Synonyms of SPF Animals

• Disease free animal
• Healthy animal
• Pathogen free animal
• Clean animal
• Caesarian derived animal

The main aim for the production of GFA is for isolation of animal free of all organism.

Introduction

Germfree (GF) animal is one which is isolated from all demonstrable living microorganisms like bacteria, virus, fungus, parasite, algae, yeast. The aim of GF work is isolation (Prevent contamination from air, water, food and environment). It can be attained mechanically and scientifically. GF animal research began in 1895 but in 1897 Nuttall and Thierfelder started to rear GF guinea pigs in their lab. Chicks are classical animal for GF research. Rats and mice are reared through reproduction. Permanent breeding colonies of these have been established.

Synonyms : Axenic animal –meaning free from strange. Gnotobiotic – known life derived from Greek word-Gnoto & biota means known flora & fauna.

Gnotobiote (Gnotobiotic animal ) : Is an animal derived by aseptic caesarian or sterile tatching of eggs that is restored & continuously maintained with Germ free technique under isolator condition in which flora & fauna is clearly defined.

Flora animal: A gnotobiote maintained under isolator condition in international association with one or more known types of microorganisms.

Accurately in the clinical diagnosis of a disease in experimental animals frequently depends on the result of laboratory examination of blood specimen from the diseased animals. A prerequisite for good experiment result is the proper collection and handling of the blood samples. If properly collected samples can not be sent to the laboratory quickly. It must be preserved or stored in such a manner that the necessary tests may be conducted. The technician/researchers

Going to collect the blood from a laboratory animal should be aware of the purpose of blood collection, the sites of blood collection, the technique of blood collection, the Use of various anti coagulants, the techniques of blood examination.

Blood Examination of Animal may be Necessary

Reproductive is fundamental property of living beings. While lower animals reproduce by simple methods, the higher animals attain greater degree of animals reproduce by complexities in the reproductive functions. Basic units (Gonads) of males and females are testis and ovaries respectively. The gonads of each sex perform dual roles:

• Formation of gametes (sperms by testis and ovum by the ovaries)
• Production of hormones.

Role of Hormones in Reproduction

Variation in Chromosome Structure

Natural variation exists in chromosome structure

Changes in chromosome structure include deletions, duplications, inversions, and translocations

The loss of genetic material in a deletion tends to be detrimental to an organism

Duplications tend to be less harmful than deletions

Duplications provide additional material for gene evolution, sometimes leading to the formation of gene families

Copy number variation is relatively common among members of the same species

Comparative genomic hybridization is used to detect chromosome deletions and duplications

Inversions often occur without phenotypic consequences

Inversion heterozygotes may produce abnormal chromosomes due to crossing over

Translocations involve exchanges between different chromosomes

Individuals with reciprocal translocations may produce abnormal gametes due to the segregation of chromosomes

Carrot

Genetic Resources

Wild carrot (Queen Anne’s Lace) grows in emperate region around the world but the extent of genetic variability in wild population has not yet been evaluated in Daucus carota or related species. Constance (1971), Heywood (1983) and Mathias (1971) have contributed much to our understanding of Umbelliferae systematics, but relatively little carrot germplasm has been collected for distribution. The cultivated carrot, Daucus carota L, intercrosses freely with almost all the wild forms. Although, no incompatibility has been observed but protandry is the main reason for cross-pollination. However, there is no barrier for selfing. Carrot variety Brasilia is resistant to nematode, Meloidogyne incognita, while species Daucus carota subsp. hispanicus has resistance to other nematode, M. hapla (Simon, 1993). The genetic material, resistant to disease and pest of carrot, which can be used for developing hybrids for abiotic stresses, are given in Table1.

10.1 Introduction

The objective of this chapter is to develop a data logger and indicator for fire detection in agriculture field. The data logger is designed with the help of Scilab. The system comprises of Ardiuini Nano, light sensor, MQ6, MQ135, IR sensor, temperature sensor, power supply. The system is designed to take sensory data on Scilab with serial communication. Fig.10.1 shows block diagram for a system. Table.10.1 shows the component list required to develop the system.

I. FILL UPS

1. Mango fruit is a _____.
2. _____ is a fleshy fruit with a single seed enclosed by a hard, stony endocarp or pit.
3. Apple fruit is a _____.
4. _____ fruit is a drupe.
5. Grape fruit is a _____.
6. Cucumber fruit is a _____.
7. Citrus fruit is a _____.

I. FILL UPS

1. Development of seed without fertilization is called _____.
2. _____ is the substitution for any sexual nuclear fusion.
3. Apomitic seed will have genetic material only from the _____ plant.
4. In _____ apomixis type, the megaspore cell undergoes the usual meiotic division and forms a haploid embryo sac.
5. The process of seed formation without sexual fusion is known as _____.
6. _____ is defined as the development of embryo from some other cell of embryo sac other than egg.
7. Haploid embryo arises from the egg without fertilization is called_____.

I. FILL UPS

1. _____ is the stage of accumulation of maximum dry matter within the seeds.
2. The stage of attainment of physiological maturity by 80% of population is considered as _____ stage.
3. In rice, during harvestable maturity the moisture content of long duration varieties will be _____.
4. For short and medium duration varieties of rice, the moisture content will be _____.
5. Rattling sound of kernel is the physiological maturity symptom of _____.
6. The harvestable maturity indices of cotton is _____.
7. The harvestable maturity indices of bhendi is _____.

I. FILL UPS

1. The major storage organ for cereals is _____ .
2. The major storage organ for legumes is _____.
3. The major storage organ in pine is _____.
4. The average protein percent present in soybean is _____.
5. The average oil percent present in oilpalm is _____.

I. FILL UPS

1. _____ refers to lack of growth due to any external or internal cause.
2. _____ refers to a state in which viable seeds fail to germinate even when given favourable environmental conditions.
3. _____ is a condition in which seed can germinate upon absorption of water.
4. _____ is otherwise known as primary dormancy.
5. A condition that develops to prevent germination after the seed has been detached from the plant is called _____.
6. Dormancy related to the embryo is called _____.

I. FILL UPS

1. _____ is the process of bringing wild species under human management.
2. _____ is referred to as taking a genotype into new environments, where they were not being grown before.
3. Introduction of plants from a foreign country is called as _____.
4. Introduction from one state to another state is called as _____.
5. When the introduced variety is well suited to the new environment it is called as _____.

I. FILL UPS

1. _____ is the increased function of any biological quality in a hybrid offspring.
2. Heterosis is otherwise known as _____.
3. _____ is the reduced fitness in a given population as a result of breeding of related individuals.
4. In _____ heterosis type, naturally occurring mutants are sheltered.
5. The progeny posses superiority over parents in vegetative growth, but reduction in yield is known as _____.
   1. Average heterosis is mathematically expressed as _____.

I. FILL UPS

1. _____ is required during raising of a seed crop to avoid contamination due to natural crossing.
2. _____ is another potent source of genetic contamination i.e., the plants differing in their characteristics from the seed variety.
3. Removal of off types is referred as _____.
4. _____ is the distance maintained between the seed crop and source of contaminant.
5. The isolation distance for cotton foundation seed production is _____.
6. The isolation distance for paddy is _____.

Varietal Seed Production Technology At a Glance

I. FILL UPS

1. _____ is the maintenance of high seed germination and vigour from harvest until planting.
2. _____ is important to get adequate plant stand in addition to healthy and vigours plants.
3. _____ seeds can be dried to 6-10% moisture depending upon the species.
4. _____ seeds cannot be dried below 30-35% moisture and should be maintained at this temperature.

1. Hallow heart in pea occurs due to

   a) Calcium deficiency

   b) Molybdenum deficiency

   c) Boron deficiency

   d) Zinc deficiency

2. Presence of pathogen on the surface of the seed is termed as

   a) Admixture

   b) Infestation

   c) Infection

   d) Contamination

1. Formal garden has a ———— layout.

   a) Odd

   b) Natural

   c) Symmetric

   d) Irregular

2. Foutain is located in —— of building.

   a) Backside

   b) Front

   c) Lateral side

   d) None of these

The term seed blending is nothing but seed mixing done based on certain principles. It is simply combining two or more seed lots of same variety or hybrid of specified quality. The blendingmayinvolve lots which mayhave different quality in terms of germination. Usually individual seed lots are much better compared to blended lots but from the point of efficient inventory management blending is advisable. The main purpose of blending is to pool all the variable lots in to a larger bulk and mix the constituents effectively so that each pocket of seed has a uniform quality.