eChapters

Introduction

The term “Remote Sensing” was coined in 1950s by Ms. Evelyn Pruitt, a geographer with the U.S. Office of Naval Research, and the term is now commonly used to describe as the science and art of identifying, observing, and measuring an object on the earth surface, without having any direct contact. This process involves the detection and measurement of radiation of different wavelengths reflected or emitted from distant objects or materials through which they may be identified and categorized by class/type, substance, and spatial distribution (National Aeronautics and Space Administration—NASA). Remote sensing technology has been widely applied in various applications such as water resources, agriculture, geological studies, forestry, land use, resource exploration, etc.

Elements of Remote Sensing

The remote sensing process involves an interaction between the incident radiation and the targets of interest, exemplified by the use of imaging systems in which the following seven elements are involved; however, remote sensing also involves sensing of emitted energy and using non-imaging sensors

Date:

Objective: To visit a newspaper organization to understand its structure and functions.

Description: A newspaper is a periodic publication containing written information about current events and other information that is of interest to the readers. A newspaper organization usually consists of the following departments:

a) Editorial department: The editorial department collects, processes and finalizes all content and images that are published in the newspaper. News/ information collected by the journalist or contributed by various writers is made readable, presentable and attractive to the readers through editing and proof reading by the editorial team.

Date:

Objective: To write an article for a farm publication.

Description: Print media can be used at the receivers’ convenience and can be preserved for future reference. It can also be used to reach the readers at a relatively low cost and the information can be verified by the reader. Due to these advantages, print media is an important form of communication, especially for development purpose. The term “farm publication” is used to refer to any publication that is prepared by an extension agency/ development department and contains information related to improvement of farming practices. Farm publications can be of various types such as leaflet, folder, magazine, newsletter, etc. Farm publications are very useful for literate farmers and can also be used by illiterate farmers with the help of others.

Date:

Objective: To write a script for a short video production.

Overview: A script is a written document that contains description of the setting, characters, dialogue, and stage directions for an audio-visual production. It is used to communicate information about actors, costumes, sets, and other aspects of production to those who will be involved in program production. While screenplay tells a story, a script (especially shooting script) mainly helps in production. Another difference between a screenplay and a script is that the former conveys what the audience will see on the screen, whereas the script guides the production team. A storyboard refers to pictorial representation of shots in a sequential order. It is very handy during shooting short videos like commercial or public service messages.

Date:

Objective: To understand shot sizes in a video production.

Overview: Shot, in video production refers to one continuous recording by the camera. It comprises of a series of frames (still images) and There are 24 frames in one second. A shot includes eeverything recorded between switching on and switching off the record button on the camera. It can contain movement of both camera and talent.

Date:

Objective: To visit a video production facility to understand the process and set up.

Overview: Video programs are often produced in a studio environment as it is convenient, easy to manage and there are very few disruptions. A typical video production facility comprises of two areas – control room and the studio floor. Director, assistant director, video and audio technicians sit in the control room. The control room has a series of monitors that display the final and camera outputs. It may also have “on air” and “preview” monitors which display images being broadcast and to check the image before recording, respectively. The control room also has a production console, which is used to select the video and audio output.

Date:

Objective: To understand basics of photography and practice taking good quality photographs.

Overview: Photograph refers to any image which is created when light falls on a photosensitive surface. With the advent of mobile phones and decreasing cost of technology, many people are taking up photography as a hobby or profession. Photographs are very useful in communication, extension and education, as they arouse interest, simplify complex ideas/ things, can be used to convey messages to illiterate people, and enhance credibility of the information. Apart from technical expertise, it is important to know the basics of composition to take good photographs.

Date:

Objective: To write a radio script for infotainment program.

Overview: Radio is an electronic audio medium that can broadcast messages to a large number of dispersed audience. Radio has several advantages as a medium of communication, especially in rural areas:

a) Radio can be carried around.

b) Low cost of production and ownership.

c) Usage is not limited by literacy and technical know-how.

d) It can be used by the listener while doing other tasks.

e) Usage is not limited by power supply.

f) Easy to operate.

Date:

Objective: To practice giving an entertainment or special occasion speech.

Overview: Entertainment and/ or special occasion speeches are short, simple and often meant to be humorous and entertaining. Special occasion speeches include welcome address, award presentation/ acceptance speech, after dinner speech, farewell speech, eulogy, etc. Entertainment or special occasion speech is often an impromptu speech i.e. they are usually given without any preparation.

Date:

Objective: To practice public speaking.

Description: Public speaking refers to communication with a large number of audience in a face-to-face situation. Public speaking differs from most other types of communication as the roles of sender and receiver are distinct and different. Also, in case of public speaking, the onus of taking the interaction forward primarily lies with the speaker. Consequently, many people find it daunting and stressful and try to avoid public speaking situations.

Date:

Objective: To make group presentation.

Description: Group presentation refers to presentation of subject matter by few or a group of people. Group presentations are fairly common in professional life and hence, one should have a mastery over how to prepare and make group presentations. If handled properly, group presentations have several advantages over individual presentations. They are more attention grabbing and interesting as the mere presence of more than one individual introduces variety into the process. Work can be shared among the team members and knowledge and resources can be pooled for better outcome.

Date:

Objective: To practice group task.

Overview: Teamwork and good interpersonal relationships are of great importance in professional life. Hence, organizations often use various methods to test qualities such as communication/influencing/leadership skills. One commonly used method is assigning group task to the candidates. Group task requires striking a balance between team goals and individual success. The team members are expected to contribute to the overall goals while furthering their individual objectives. Additionally, the evaluator can observe leadership skills, team spirit, communication skills, analytical and decision-making skills of the group members without being intrusive. One can also judge the role for which a particular member is best suited. The time assigned for a task-based group discussion/ group task can range from 10 to 60 minutes depending upon the complexity of the task.

Date:

Objective: To practice case-based group discussion.

Overview: A case is a detailed description of anything that has happened in real life. In a case, real names are often changed to ensure confidentiality and protect the privacy, but it is not a fictitious account. A case presents a situation, or a problem and the learner is expected to suggest a solution based on the information given in the case. Case based group discussion is often used to test the analytical and reasoning ability of the participants. While participating in a case-based group discussion, the students should start by recognising and defining the problem. This is followed by listing as many solutions as possible with contributions from all the group members. In the next step, each solution is evaluated critically by the group members keeping in mind the information/ data/ facts given in the case. Finally, the best possible solution is put forth by the group. It is important to remember that there are no right or wrong solution as any option is often a trade-off.

Date:

Objective: To practice group discussion and understand the do’s and don’ts of group discussion process.

Overview: Group discussion is a form of discourse, which occurs when more than two people recognize a common problem, exchange and evaluate information and ideas. Their efforts may be directed towards a better understanding of the topic or solving a problem. Group discussion occurs in a face-to-face or co-acting situation. It is a democratic method in which equal opportunity is given to every participant to express his/ her opinion. In formal settings, group discussion takes place under the direction of leader. Group discussion is often used by academic institutions or employers to assess certain traits and skills of the participants. The number of participants usually is around eight, but there is no hard and fast rule. When being used as a method of assessment/ evaluation, the group is given a topic.

Date:

Objective: To prepare a projected audio-visual aid (power point presentation).

Description: Audio-visual aids that are magnified and projected on to a screen are called as projected audio-visual aids. There are three methods of projection:

a) Direct projection: In this case, light rays from the source of illumination pass through condenser lens, then hit the object and go through objective lens and fall on the screen. Slide projector is an example of direct projection.

Date:

Objective: To prepare a non-projected audio visual aid (poster).

Description: Poster is a placard displayed in a public place to raise awareness. A well-made poster must attract attention of passers-by and motivate them to take action. Here are a few tips for making effective posters:

a) A good poster should rely on pictures rather than words. While there is no strict rule regarding the number of words, try to use no more than 10- 12 words.

b) The font size should be big enough to read from a distance as posters are displayed at public places. If there is a variation in font size, increase the size as you move from top to bottom. In addition, while choosing colours, keep the background against which the poster will be displayed in mind.

Date:

Objective: To prepare a non-projected audio-visual aid (folder).

Description: Folder is a single thick sheet of paper, which is usually folded once or twice. Folder contains essential information about a place, event, etc. which is printed on a good quality paper. A folder is prepared as and when needed and is distributed free of cost.

Date:

Objective: To prepare a non-projected audio-visual aid (chart).

Description: Audio visual aids are media that can make communication more effective through use of sound and visuals. They are of two types – projected and non-projected aids. Non projected aids are visual materials that are used as it is by the instructor and do not need any device or equipment. Examples include charts, posters, leaflets, flash cards, etc. They are easy to prepare, carry and use. They can be prepared as and when needed and multiplied at low cost.

Date:

Objective: To understand the characteristics and limitations of language.

Description: Language is the structured system of symbols, which are called as words. Semantics refers to the study of the relationship between symbols and their meaning. All languages are arbitrary as the word symbol is not the thing it represents (i.e. if all of us agree, we can refer to a “cat” as “donkey”, without turning it into one!). Words can be both abstract (things that cannot be sensed directly) and concrete (things that can be experienced through sensory organs).

Date:

Objective: To understand gap in communication process.

Description: Communication is never 100 percent efficient. In other words, even an ideal situation does not lead to complete and full understanding between the sender and receiver. Often, the receiver may understand the message differently or receive only a part of the message. This happens as every individual has a different frame of reference. Frame of reference is the set of experiences, beliefs and values which influence a person’s behaviour and acts as a benchmark against which all new stimuli are interpreted. Based on the frame of reference, every individual develops a perception. Perception is the process of receiving and interpreting stimuli.

Date:

Objective:To understand the concept of perception and its role in communication.

Description: Human body receives stimuli through various sensory organs and the brain processes these stimuli and assigns meanings leading to a reaction. This process of organizing, identifying and interpreting of sensory stimuli leading to an understanding of the environment is called perception.

Date:

Objective: To practice giving appropriate feedback.

Description: Communication is a cyclic process. The message originates from the source and is reaches the receiver. The receiver then decodes the message (i.e; makes sense)and sends a message back to the sender to convey his/her understanding or agreement with the message that has been received. Feedback not only completes the communication cycle, but also takes the process forward.

Date:

Objective: To understand the importance of paralanguage in verbal communication.

Description: Paralanguage is the variation in voice and includes vocal characteristics such as pitch, range, resonance, tempo, etc. Though it is an integral part of verbal communication, it is quite distinct from the actual signs and symbols that are used by the communicator.

Date:

Objective: To understand the significance of non-verbal communication.

Description: Non verbal communication refers to communication without the use of words. It can encompass a wide range of communication behaviour from body language to choice and use of architectural objects. It is used for repeating, substituting, complementing, regulating verbal messages and deceiving. Some of the common non verbal means of communication include: Gestures: Gestures are movement of body parts and kinesics refers to the study of body movement.

Date:

Objective: To understand communication process and its elements.

Description: Communication is the process by which a person shares knowledge, feelings, ideas, and information, etc. with another person(s) in such a way that each gains a common understanding about the meaning and intent of the message. Major functions of communication include sharing information, issuing instructions, persuasion and motivation. There are several elements in communication process. Anyone who takes the initiative and starts the communication process is called the source or communicator. The intended recipient of the communication content is called as the receiver. Any sign or symbol which has the same meaning for the source and the receiver is called is the message. Receiver is the intended recipient of the message and what the communicator hopes to accomplish is the objective of communication process.

Date:

Objective: To get acquainted with classmates.

Description: This is the first lab activity and has been designed to make the students feel comfortable in their new surroundings. The aim is also to help them get acquainted with their classmates as they are still new to each other. This short fun activity will help them mingle and set the tone for upcoming activities.

4.1 Introduction

According to Ministry of Information and Broadcasting (2019), presently there are 275 operational community radio stations. There are ten communi radio stations in the state of Uttarakhand. One is called Kumaon Vani which is licensed to the well known environmental non-profit group called The Energy andResourcesInstitute (TERI) based in Mukteshwar district.The otheris called Radio Khushi licensed to Guru Nanak Fifth Centenary School and based in Mussoories and third is Hello Doon licensed to National Institute of Visually Handicapped (NIVH) and based in Dehradun. The fourth is Hevalvaani in Chamba (Tehri Garhwal) whuch is licensed to NGO Aastha Jan Kalyan Evam Vikas Samiti . The fifth is Pantnagar Janvani licensed to Govind Ballabh Pant University of Agriculture and Technology (GBPUAT) and based in Pantnagar. The six is Himgiri ki Awaaz licensed to Himgiri Nabh Vishwavidyalaya (University of Sky) in Dehradun. The seventh is Radio Zindagee licenced to Graphic Era Educational Society. The eight is Radio Dehradun licenced to one NGO name Swantarta Senani Lokbandhu Ram Murti Pawsey Sewa Nyas Trust. The ninth is Hello Haldwani which is licenced to Uttarakhand Open University. The tenth is Mandakini Ki Awaz in Rudra Parayag which is licenced to NGO Mandakini Ki Awaj Sewa Samiti.

3.1 Introduction

Government of India has made significant impact in empowering girl child through initiation of various schemes viz; Beti Bachao Beti Padhao, Sukanya Samriddhi Yojana etc. After initiation of such types of programme for girls, Child Sex Ratio was increased during 2021-22 i.e; 929 females per 1,000 males. Recently Government of India has initiated new innovation in Beti Bachao Beti Padhao programme. Present Paper aim is to discuss the new innovations during 2017 to 2022 under this scheme.

In India under the leadership of Prime Minister Shri Narendra Modi foresee rural India as ability laden with great prospective to speed of Sustainable Development Goal. Looking at splurge of schemes targeting girl child like Beti Bachao Beti Padhao, Sukanya Samriddhi Yojana, Ladli Yojana and several other schemes empowerment of girl child seems to be heart of a plan to attain dream of Aatma Nirbhar Bharat.

2.1 Introduction

Women and Girls are born in to social and cultural system steeped in inequality and discrimination. They receive an unfair shape of opportunities, attentions and resources from the moment of their conception. One of the most inequality is Violence against Women and Girls [VAWG]. Violence against Women is a serious obstacle to development, peace and equality. It dehumanizes women and nullifies human rights and fundamental freedom. Present Article explains types of domestic violence and Government support system against Gender based Violence.

Violence againstWomen [VAW]is globally pervasive.It existsin every country, cutting across boundaries of culture, class, caste, education, income, ethnicity and age. These trends are also reflected in the status of women in India. Census data shows that while the sex ratio has risen since the last decade, it is still low at 933, the juvenile sex ratio in India hasreached an all time low of 927. Census data 2001, official statistics from the National Crimes Record Bureau [NCRB] reveals a trend of rising crimes Record Bureau [NCRB] reveals a trend of rising crimes against women. Cultural practices in India such as dowry, child marriage, sati, the Devadasi tradition and ill treatment of widows enhance girls and women’s vulnerability to the experience of violence. Gender differential treatment related to nutrition, health care, education, mobility and other life opportunities places girls and women at a higher risk of gender violation.

1.1 Introduction

The state of Uttarakhand was formed in November 2000. At the state level, it is the Department of Women’s Empowerment and Child Development that has overall responsibility for formulating and implementing schemes relating to Women’s Empowerment. As the nodal agency, the responsibility for preparing the Women’s Policy was given by the Government of Uttarakhand to Department of Women’s Empowerment and Child Development. Presently the Government of Uttarakhand is running various schemes/programmes through the different departments of the Government to bring about women’s development and their empowerment. For lack of an overarching policy that would have provided a common understanding and a unified vision, each department has defined and operationalized empowerment through its own prism and from its own perspective and understanding. In the case of some departments, the stated goal is not to bring about women’s empowerment, but rather, the emphasis is on bringing about their overall efficiency and economic development.

2.1 Introduction

Women entrepreneurs may be defined as the women or a group of women who take initiative to set up a business enterprise and to run it smoothly. According to Government of India, women entrepreneur is defined as an enterprise owned and controlled by a women and having a minimum financial interest of 51 per cent of the employment generated in the enterprise to women. Women entrepreneur are those women those women who generate business ideas or select the best opportunity, mobilize resources, combine the factors of production, undertake risks and operate the enterprise in the most effective manner with a view to earning profit.

The verb empower means to bestow power. Empowerment of women implies creating conditions for enabling the women to be motivated for task accomplishment by taking personal responsibility. In the context of empowerment through enterprise, empowerment of women involves access to resources and markets, actual ownership and active control. These factors act as the bullwork for empowering women through enterprise.

2.1 Introduction

Empowerment means change, choice and power. It is a process of change by which the individuals and groups with no power and ability to make choices that affect their lives. Women empowerment is a stage of acquiring power for women in order to understand right and to perform her responsibilities towards oneself and others in effective way.

Empowerment is a multi-faceted, multi-dimensional and multi-layered concept. Women's empowerment is a process in which women gain greater share of control over resources- material, human and intellectual [knowledge, information, ideas] and financial resources and control over decision-making in the home, community, society, nation and to gain `power'. According to the Country Report of Government of India, "Empowerment means moving from a position of enforced powerlessness to one of power". In this process, women should be empowered socially, economically, educationally and politically that can help them take Self-decision regarding education, mobility, economic independency, political participation, public speaking and awareness to exercise rights.

1.1 Introduction

Gender mainstreaming, a concept that emerged from international frameworks and global development agendas, is a strategy aimed at achieving gender equality by integrating gender perspectivesinto allstages of policy-making and organizational practices. Originating from the recommendations of the 1985 Third World Conference on Women in Nairobi and formally endorsed at the 1995 Fourth World Conference on Women in Beijing, gender mainstreaming has evolved into a fundamental approach within international development efforts. It was adopted as a methodology by ECOSOC in 1997, marking a significant commitment across the United Nations system to advance gender equality goals comprehensively.

In essence, gender mainstreaming is not merely about inclusivity; it represents a proactive stance towards challenging and reshaping societal structures and ideologies that perpetuate gender disparities.

3.1 Introduction

To remove the inequality in technology development and its use, the concept Gender and technology arises. Economic resources should be equally distributed among the men and women. Technology can also help challenge and break gender stereotypes and biases that often limit women's potential. First of all, much equipment tends to be gender-typed. There are machines and tools suitable" for men as saws, trucks, wrenches, guns and forklifts. Some other technologies are "suitable" for women as vacuum cleaners, typewriters and food processors. The main focus of year 2023 is on innovation and technology for gender equality. Innovation and technological change are deep shifts reshaping life in the twenty-first century. Technological progress allows for unprecedented advances with the potential to improve social, economic and political outcomes for all.

 

2.1 Gender Analysis

Inequalities between men and women are becoming more visible and glaring particularly in developing countries of the world triggering serious concern over developed experts. UNESCO statistics reveals that women perform 75 per cent of work, earn 10 per cent of the income and own 1 per cent of property. The 3rd UN Millennium Development Goal (MDG) has called for gender equality and the components of women to battle poverty, hunger and disease and to restorative development that is precisely sustainable.

The Beijing Platform for Action (1995) very well support this fact in form of twelve objectives and action points viz., women and poverty, education and training for women, women and health, violence against women and armed conflicts, women and the economy, women in power and decision making, institutional mechanism for the development of women, human rights of women, women and media, women and environment, and the girl child. Prior to the above declaration there were First, Second and Third World Conference on Women at Mexico (1975), Copenhagen (1980) and Nairobi (1985), respectively.

1.1 Introduction

Gender permeates every facet of society, influencing power dynamics, access to resources, and opportunities. Understanding gender requires not only defining its conceptual framework but also acknowledging how these concepts manifest in everyday life, often creating deep divisions. Gender divides whether in education, employment, health, or political participation are enduring realities that reflect the unequal distribution of opportunities and rights between men and women, as well as between those who identify beyond the binary. These divides are more than social phenomena; they have concrete economic, political, and developmental impacts. Recognizing the persistent gaps in access and outcomes between genders has led to the development of gender-sensitive policies, such as gender budgeting. Gender budgeting is a critical tool for addressing these divides by ensuring that public resources are allocated equitably to benefit all genders. By focusing on the differential impacts of policies and budgets on women, men, and gender minorities, governments and organizations can work toward creating more inclusive and fair societies.

This chapter will introduce key gender-related concepts that form the foundation for understanding how gender influences social, economic, and political life. It will explore the roots and ramifications of gender divides across various sectors and explain how gender budgeting is employed as a strategic approach to reduce these disparities. Through this exploration, we will highlight the importance of integrating gender perspectives into policy and resource distribution to foster equity and social justice.

2.1Introduction

Agriculture includes both self-employment and wage employment. This require precise information about who does what. Majority of farmers have small Land holding and landless labourers. This needs understanding of activity performance of men and women. Gender-based division of labour is universal, but culture and community diversities cause differentiation. Gender, therefore, has to be recognized as the social characteristic that cuts across caste, class, occupation, age and ethnicity. It is gender that differentiates the roles, responsibilities, resources, constraints and opportunities of women and men in agriculture for which precise gender information is the need of the day. Building gender into agricultural development will lead to: Building inherent strength of women and men to mutually learn, Overcoming gender based prejudices etc.

2.2

Importance of Gender in Agriculture Agriculture play an important role in livelihoods of the rural poor and achievement of the Millennium Development Goals (MDGs). Agriculture can be the engine of growth and is necessary to reduce poverty and food insecurity. The changes in Agriculture sector generate new sources of opportunities for livelihoods and food security, they also pose significant qualms. Equity distresses are being raised.

The concept of gender, distinct from biological sex, serves as a lens through which societies organize individuals and define roles, expectations, and relationships. Gender is not merely a matter of identity but an intersectional construct shaped by cultural, social, economic, and political influences. It reflects how different societies understand and express masculinity, femininity, and other gender identities, often dictating the power dynamics, opportunities, and constraints placed on individuals.

The understanding of gender has evolved significantly over time. Initially, it was viewed as a binary construct aligned strictly with biological sex; however, modern perspectives recognize gender as a fluid and dynamic concept, shaped by individual experiences, social interactions, and institutional norms. This evolution has brought about greater recognition of diverse gender identities, challenging traditional binaries and expanding the conversation to include non-binary, transgender, and gender non-conforming identities.

At the heart of gender are the societal structures that enforce roles and expectations. These structures differ across cultures and historical periods but commonly emphasize the division of labor, power, and resources between men and women. Gender not only influences individual identity but also informs how societies function, shaping everything from economic participation to political representation.

4H: An agricultural club for children that features hands-on learning

Abiota: The nonliving component of an ecosystem, including the soil, water,

and air.

Aborigines: The original or native inhabitants of a country or region.

Absorption Losses: Loss of water from a canal or a reservoir by capillary

action and percolation and in case of canal during the process of delivery.

Absorptive capacity: The maximum amount of waste that can be absorbed

by the environment.

Acclimation: Adaptation to changing or new conditions.

Acid precipitation: Rain, snow, fog, or dew containing sulfuric and nitric acids

produced by fossil fuel combustion.

Acid soil: A soil which is deficient in available bases, particularly Ca and which

give an acid reaction when tested by a standard method.

Acre foot water: The amount of water that would cover an area of land to a

depth of one foot assuming no seepage evaporation and run off.

Acre inch day: Term used principally in irrigated section of united state for

measuring quantity of flow of water. It is equal to a flow which will cover

one acre to a depth of one inch in a 24 hours period or 0.042 cubic feet per

second.

Acre inch: It is a measure of quantity of flow of water and is equal to the flow

which will cover one acre to a depth of one inch.

Acre: (43560 sq. ft) an area of land about 220 feet long and 198 feet wide.

Active solar heating: The use of solar panels to collect and concentrate the

sun’s energy for heating.

Adaptation: Biological modification that allows species to better exist in a

specific environment.

Additives: Chemicals added to food, often con- sidered to represent a threat

to human health.

A

Adenocarcinomas 3, 7

Adenomas 7

ALDH1 17, 18

Anaplastic carcinomas 7

B

Babesiosis 1

Bcl 17, 21, 23

Benign tumors 3, 5

Biomarkers 17, , 29, 30, 31

BRCA 31

C

Canine 1, 2, 5, 7, 8, 9, 10, 11, 12, 13, 17, 19,

19, 22, 23, 24, 27, 29, 30, 32, 33, 34

Canine distemper 1

Canine Mammary Tumors 1, , 5, 7, 8, 13, 17,

19, 29, 32, 33

Canine parvoviral diarrhoea 1

Carcinogenesis 10, 25

CHEK2 15

Classification and Grading 7

Companion animals 1, 9

COX inhibitors 21, 26

Cyclooxygenases 23, 26, 35

D

Deracoxib 21, 26

Diagnosis 19, 20

Doxorubicin 21

E

EMT 17, 25

Epidemiology 11

Estrogen receptors 20

FGFR2 15

Agostini M., Enzo M. V., Bedin C., Belardinelli V., Goldin E., Del Bianco P., and Nitti D.

(2012). Circulating cell-free DNA: a promising marker of regional lymphnode metastasis

in breast cancer patients. Cancer Biomarkers 11 (2-3), 89-98.

Badowska-Kozakiewicz A. M., and Malicka E. (2012). Immunohistochemical evaluation of

expression of heat shock proteins HSP70 and HSP90 in mammary gland neoplasms in

bitches. Polish Journal of Veterinary Sciences 15 (2), 115-18.

Beffagna G., Sammarco A., Bedin C., Romualdi C., Mainenti M., MolloA. and Zappulli V.

(2017). Circulating cell-free DNA in dogs with mammary tumors: short and long fragments

and integrity index. PLoS One 12 (1), e0169454.

Birdi R., Kumar B. S., Gupta K., Kashyap N., & KumarA. (2019). Circulating level of heat

shock protein 27 is elevated in dogs with mammary tumors. 3 Biotech 9 (6), 1-9.

Campos L. C., Lavalle G. E., Estrela-Lima A., Melgaço de Faria J. C., Guimaraes J. E., DutraA.

P. and Cassali G. D. (2012). CA 15.3, CEA and LDH in dogs with malignant mammary

tumors. Journal of Veterinary Internal Medicine, 26 (6), 1383-88.

Mammary tumors are the most common type of neoplasia found in female dogs with high percentage of malignancy and rate of mortality. The rate of incidence of these mammary neoplasia and dysplasia is continuously advancing nationally and internationally over the last 45 years. There are different risk factors such as age, diet, hormonal exposure and breeds, which are required to be monitored in order to control the incidence of CMTs. In addition to conventional therapies such as surgical excision, radiotherapy and chemotherapy, additional precision therapeutic agents targeting TKIs, p53 tumor suppressor gene, cyclooxygenases, epithelial-mesenchymal transitions and various hormones/ hormonal receptors could be beneficial for the treatment of CMTs. Scientific efforts are also being made to develop different biomarker panels and tests for prognosis/diagnosis of the diseases as well as to develop vaccines against CMTs.

In GADVASU, under the DBT sponsored project on “Development and evaluation of serodiagnostic assay for timely diagnosis and prognosis of mammary tumors”, we have generated and used hyper-immune sera raised against certain canine recombinant mammary tumor associated proteins for the standardization of sandwich and indirect ELISA for serodiagnosis of canine mammary tumor. The sensitivity and specificities of the assay’s ranges between 85-88% and 80-85%, respectively. However, an antibody against a single marker when used for different histotypes of CMT, its specificity reduced significantly. Multiplex assays are capable to detect many specific antigens in a single run whereas a traditional ELISA is used only to detect a single antigen. It has high-throughput potential, providing more results per sample, with lower sample volumes and with utmost sensitivity and specificity. Attempts are being made to increase the sensitivity and specificities of the assays by multiplexing various markers together (Fig.4).

Although there have been multiple biomarkers for mammary tumors both in human and canines reported over the time (Table 3), but no absolute biomarker for diagnosis and prognosis of CMT has been found yet. Out of several studies on clinical applications of different mammary tumor biomarkers, some showed a particular promising biomarker, on the other hand controversial results also existed for the same. For example, HER-2, which is one of the most prominent bio-marker both in HBC and CMT. HER-2 overexpression is associated with poor prognosis and reduced overall survival of the subject, but there are studies available which suggest no difference in HER-2 expression between benign and malignant tumors and studies showing HER-2 expression suggesting prolonged survival (Kaszak et al., 2018). Various biomarkers of mammary tumors are not mammary tumor specific such as p53, COX-2, PCNA etc. These markers undergo alterations in their expression level in all tumors. By keeping this in mind we need to device a panel of different markers to ensure the correct prognosis and diagnosis of canine mammary tumors. There are limited studies and only few commercial kits (for human) available using such combinations (Table 4).

One of the most promising areas of veterinary research these days is cancer immunotherapy and the development of CMT vaccines. DNA vaccines, also known as gene vaccines, are designed to transfer functional genes to target cells, allowing them to express functional proteins. Because endonucleases may easily access bare DNA, it requires a reliable and safe delivery route, which might be biological or non-biological. Different delivery strategies have been tried in human breast tumors to transport tumor antigens such Mucin-1 (MUC-1), Twist, HER-2, mammaglobin-1 and p53, as well as immunostimulatory molecules like IL-12 and IL-6. There have been various studies/ trials in canine animals using different molecules like IL-12, HER-2 and IFN-γ as vaccine candidates, with no clinically relevant outcomes or longlasting cell mediated and humoral immune response outcomes

In addition to the conventional treatment methods i.e., surgery, chemotherapy and radiotherapy, there are some adjuvant targeted therapies which block the growth of cancer by altering the individually expressed specific molecules responsible for tumor cell proliferation, survival, metastasis or microenvironment. The majorly studied adjuvant therapies include use of hormones, tyrosine kinase receptors, anti-cyclooxygenases, anti-tumor suppressor gene p53 and epithelial-mesenchymal transition inhibition.

The main aim of hormonal therapy (Table 2) is to inhibit the hormone induced stimulation of carcinoma cells, which could be achieved either by ovariohysterectomy or different treatments like use of specific estrogen receptor modulators (SERMS), aromatase inhibitors for suppression of estrogen synthesis etc. A commonly used estrogen receptor (ER) antagonist drug tamoxifen for treatment of ER positive breast cancer in women, has also been tested against CMTs. However, it is not a viable treatment option in dogs due to associated side effects. Ovariohysterectomy is considered as a better and practical solution. The use of hormonal therapy in CMTs is based on the evidence of hormone dependency of these tumors as well as lessons learnt from hormonal therapy outcomes from human breast cancer. This therapy significantly ameliorates the recurrence of breast cancers and prolongs the survival rates in women having breast cancers positive for hormone receptors. However, the results of the canine investigations, which are all retrospective and non-randomized, are contradictory. Detailed research and prospective randomized trials, where effect of OHE is studied in context of hormone receptor status, are required to determine the role of hormonal therapy in CMTs.

Surgical removal is the routine treatment method for mammary tumors, however, there is no consensus on the optimal procedure. There are supporting factors for every surgical procedure conducted for mammary tumors including lumpectomy (removal of tumor only), mastectomy (affected gland removal only), radical mastectomy (complete mammary gland and associated lymphatic tissue removal) and modified radical mastectomy (removal of complete mammary gland, lymph nodes and associated chest muscles).The more extensive procedures do not increase survival in dogs when compared to the other procedures, and the benefits of the simpler procedures are clear. Further, the radical mastectomy increases the disease-free interval but not the survival duration in cats.

A thorough history with complete physical examination including careful palpation of the mammary glands should be performed. As there are chances that dogs may be suffering from any other systemic ailments during middle to old age. In dogs with multiple tumors, all tumors should be excised and their location and size should be noted. Histopathological examination of the excised tumors should be carried out to classify mammary gland tumors. Mammary tumors are classified based on some of the parameters i.e., tumor size, lymph nodes, metastasis. Therefore, to stage a dog with mammary tumors information of these parameters needs to be collected and recorded. If lymph nodes are palpable and/or clinically enlarged, fine-needle aspiration should be performed for cytological evaluation. The technique is less invasive but with higher accuracy. Thoracic radiographs remain the mainstay for diagnosing the metastatic condition. The mammary tumors most commonly metastasize into lungs in dogs. The staging of CMTs becomes necessary as it is likely to confer some prognostic significance and provide guidance to veterinarians to make treatment decisions.

There are countless biomarkers present in cells related to and determining the progress of a disease. Identifying these markers could aid in detecting cells with metastatic potential (Ramaswamy et al., 2003).

The elevation of cyclo-oxygenase (Cox-2) expression was observed in CMT cases through enzyme immunoassay (Queiroga et al., 2005). In further study, enhanced expression of BRAC1and BRAC2 genes, certain enzymes such as matrix metalloproteinases (MMP-2 and MMP-9) by immunohistochemical examinations, and Bcl-2 & Bcl-XL elevated level in dogs with mammary neoplasm have been reported by Queiroga et al. (2011b).