eChapters

Liquid nitrogen and cryocan

Several cryogenic agents like solid carbon-di-oxide (-79^ºC), liquid air (-183^ºC), liquid helium  (-263^ºC) and liquid nitrogen (-196^ºC) have been used in many countries for freezing and preservation of bovine semen. Liquid nitrogen is fourth coldest substance known and its small amount is converted into a very large amount (near about 700 times) of its volume when exposed to atmosphere. It is a colourless, odorless, non-irritant, non-toxic, non-caustic, non-corrosive, non-inflammatory and inert gas having boiling point of -196^ºC. Being a liquid it comes into good contact with the surface of the semen straws, constant storage temperature is maintained throughout the straw, and due to these properties LN2 is most widely used refrigerant for semen cryopreservation in the world. 

There is no rapid direct test to determine fertility of semen sample. During freezing and thawing of semen, 20-80% of the spermatozoa become immotile or dies. In addition to this, there may be damage to spermatozoa by disintegration of sperm cell membrane and acrosome leading to loss of intracellular enzymes. The only reliable method to evaluate the semen is to inseminate a cow and get a calf at the end of gestation period. Now-a-days new methods have been developed for in-vitro assessment of fertility of spermatozoa. Some of these methods are exhaustive, time consuming, need for a full-fledged analysis and difficult under field conditions.

1. AI with liquid semen

The ampoule is thawed in ice water for 6-7 minutes. Then, the ampoule is dried and cut. The semen is drawn in a sterilized glass pipette (40-42 cm x 5 mm length diameter) fitted to a sterile glass/plastic syringe (2-5 mL) filled with 1 mL of semen with a rubber connecter. Artificial insemination is done by recto-vaginal method after securing cow/buffalo in standing position in the trevis.

The reproductive efficiency in dairy herd is one of the important factor in determining total production and profitability. To remain profitable, dairy cows must reproduce at greatest efficiency and should calve every year (12-13 months). The measure of fertility in a herd is the calving rate (percentage of live, normal calves born). However, it is also necessary to measure fertility based on services per conception. In a healthy herd in which fertile bulls are used, an average of about 1.6 to 1.7 services is required per conception. The number of services per conception is particularly useful for analyzing costs and in comprising the A.I. quite similar to that obtained through natural service in well managed herds. Another measure of fertility is the non-return rate that is calculated from insemination records. Non-return rate is the percentage of cows that are not subsequently rebred within a defined period (60 to 90 days) following an insemination. On an average, about 70% percent of cows inseminated fail to return for service after first service on a 60 to 90 days non-return basis.

The spermatogenesis is a continuous process in male animals but affected by following factors. 
    
1.     Genetic makeup of male animal: Certain sperm defects like Diadem defect, knobbed sperm, decapitated sperm, sterilizing tail stump, corkscrew mid piece and narrow head are inherited defects and reported in certain males. Few inherited genetic factors are also associated with poor libido and poor fertility. Inherited sperm defects affect sperm motility, fertilization and embryo formation.

The progress of AI organization depends upon the type of service over and above that initially expected by the members. A well-conducted field service is very important in expanding the growth of any breeding organization. It includes activities as publicity, cooperation in health control, production testing and culling and progeny appraisal, the holding of demonstrations and open house and participation in cattle shows. The salary of a technician with bonus incentive encourages to do work of a high order. The semen costs per inseminated cow can be reduced by maintaining highest quality standards in producing, processing and shipping, by increasing number of cow serviced and by cutting all unnecessary costs. When breeding efficiency is high, the required amount of semen is kept at a minimum. An increase in cows inseminated is a key factor in keeping an organization in the back.

The information in respect of reproductive parameters and events are recorded in respect of following points.
    
1.    Date of semen collection, evaluation, preservation and semen distribution from different bulls 
    
2.    Fertility status of semen 
    
3.    Reproductive performance of females 
    
4.    Work of inseminators

Immediately after use, all sorts of items are thoroughly washed with water and the rubber catheters are washed with the force of tap water to remove the residual diluted semen. The glass wares are kept in 1% chromic acid solution for overnight to remove cloudiness of glass surface. Then the articles are washed with lukewarm laboratory grade detergent (ex. Labolene®) with a brush to make the articles grease free. All the items are thoroughly washed several times under distilled water and kept inverted position in a clean trough then the openings of glassware wrapped with aluminum foil / paper.

Breeding soundness examination can be used as a diagnostic aid in herd fertility problems. After breeding soundness examination, a bull is classified as a satisfactory potential breeder, a questionable potential breeder or an unsatisfactory potential breeder. Members of the Society for Theriogenology developed the concept of a breeding soundness examination as mentioned below.

The first buffalo AI progeny is reported at Allahabad in August 1943. Although AI is commonly used as a tool of breeding in buffaloes and complaints have appended that conception rate is not satisfactory. There are differences in the anatomy of the sexual organs, sexual physiology and general breeding behaviour that have to be known and taken into consideration when the same technique of AI used in cattle is applied in buffalo breeding. It explains the lower semen production capacity of male buffaloes compared to the cattle bulls. The buffalo bull’s penis is shorter and thinner than penis of the cattle bull. There is a pronounced tapering of the penile end and glans penis is rather poorly developed. The thrust is shorter and less forceful than the bulls. The sheath is tighter and closer to the abdomen than zebu cattle. These anatomical differences are of significance in the semen collection work and type of artificial vagina to be used. The weight of testis and epididymidis in the male buffalo is only ¼ of what it is in cattle. In addition, the seminal vesicles and the prostate are considerably smaller.

Artificial insemination in horses is being practiced since many years in Russia and China for genetic up-gradation. The equine artificial insemination industry has evolved a system in which mares are inseminated with fresh or cooled semen (500 million motile sperm) every 48 hours until estrus is no longer detected or until ovulation is detected by trans-rectal palpation or ultrasonography of ovaries respectively. The advantages of AI include:

Artificial insemination (AI) technique in pigs is being performed since several decades in many countries but not widely accepted technology as in cattle due to reasons like sub-standard method of semen cryopreservation and lower fertility rate. The main idea is to increase the breeding efficiency by utilization of superior boars. The number of boars needed for breeding purposes can be reduced by use of AI and reduced transmission of infectious diseases. A minimum of 15 to 20 sows can be inseminated after dilution over a 2 days period with an ejaculate from a boar. Artificial insemination had considerable impact worldwide in its application to commercial pig production. The main advantage of using fresh liquid boar semen is that fertility is maintained even with low number of spermatozoa in the inseminate. AI provides unique opportunity for the genetic improvement of pig industry. Using fewer than 1 million sperm cells per breeding unit can achieve conception rates with liquid semen similar to those with frozen thawed semen containing approximately 15 million sperm and reduced fertility makes cryopreservation an uneconomical option in boars.

Artificial insemination Technology is a high cost and labour intensive technique in sheep and In India, sheep are reared by economically weaker sections of the society and any advanced technique may take much time to reach their flocks.  Considerably more research work has been done in AI with sheep than with goats. However, the equipment and techniques are very similar for the two species. Goat semen may be stored and frozen more successfully than ram semen.

Artificial insemination technology is a valuable tool to inseminate 2000 to 3000 does by frozen semen instead of breeding 40 to 50 does by natural service in a year. Goat semen may be stored and frozen more successfully than ram semen. The buck will be sexually mature at 6-7 months of age.

Castration means removal of the testicles and there are two methods of castration in domestic animals viz. closed and open method.

1. Closed method

In closed method, without incision, the spermatic cord is crushed where thrombus is formed which arrest blood flow to the testicles resulting in gradual atrophy of the testicles. ex. Burdizzo method

1.1     Prokaryotic 
    
1.2     Eukaryotic
    
1.3     Cell orgenellie

2.1     Regulation of the Cell Cycle 
    
2.2     Mitosis
    
2.3     Meiosis

DNA (deoxyribonucleic acid), the giant molecule that carries genetic information in living things, is made up of just a few chemical building blocks that bond together in very particular ways. A typical molecule of DNA consists of two strands that are linked together. A segment can be visualized as a ladder-like structure:

The DNA as made of two strands, a left and a right, which are linked in the middle. In reality, since the ladder is twisted, there really is no “right” or “left,” but we use that terminology here since it applies to the model graphs. The square shapes and diamonds you see in the middles of the “rungs” are the links, which are meant to represent the different chemical bonds between the two strands of DNA.

4.1 DNA: The word means
    
4.2 Double Helical Structure: The Watson and Crick Model
    
4.3 Family of DNA 
    
4.4 The Chromosomes

5.1 Replication
    DNA Replication in Bacteria
    DNA Replication in Eukaryotes
    
5.2 Transcription

5.3 Translation

6.1 Prokaryotic Gene Expression
    
6.2 The lac  Operon
    
6.3 The trp  Operon

7.1 Gel Electrophoresis
    
7.2 Polymerase Chain Reaction (PCR)
    
7.3 Restriction Fragment Length Polymorphisms (RFLPs)
    
7.4 Random Amplification of Polymorphic DNA (RAPD)
    
7.5 Amplified Restriction Fragment Length Polymorphism (AFLP)

8.1 Sub Cloning
    
8.2 Cloning into a Yeast Artificial Chromosome (YAC)
    
8.3 DNA Libraries

9.1 Methylases
    
9.2 Polymerases
    
9.3 Nucleases
    
9.4 Ligases
    
9.5 Phosphatase
    
9.6 Topoisomerase
    
9.7 Restriction enzymes

10.1 Plasmids
    
10.2 Retrovirus

11.1 Total DNA Extraction by cTAB Method
    
11.2 Extraction, Purification Estimation of DNA
    
11.3 Potassium Acetate Procedure
    
11.4 DNA  Estimation
    
11.5 Restriction Enzymes

Acetic acid            
    

Molecular weight    60.05        
    Melting point (^ºC)    17        
    Boiling point (^ºC)    118        
    Flash point (^ºC)    40        
    Auto-ignition point (^ºC)    426        
    Specific gravity at 20^ºC    1.05        
    Molarity of the concentrated acid (M)    17.4    
    1 M solution    57.5 ml concentrated acid per litre

1.1    Introduction

1.2    Histories and Development
    
1.3    Components of Drip Irrigation System
    
1.4    Types of Drip System
    
1.5     Advantages and Disadvantages
    
1.6    Evaluation and Futuristic Approach of Drip Irrigation in India Questions & Problems
        
References

2.1    Reynolds Number
    
2.2    Darcy-Weisbach Equation
    
2.3    Hazen-William Formula
    
2.4    Hydraulic Characteristics of Distributors
    
2.5    Manufacturing Variation of Distributors
    
2.6    Irrigation Uniformity and Efficiency
        
Questions and Problems
        
References

3.1    Crop Water Requirements
    
3.2    Water Distribution in Soils and Wetting Pattern
    
3.3    Selection of Number of Distributors per Plant
    
3.4    System Capacity
    Questions & Problems
        
References

4.1    Hydraulic Formulae/Head Losses in Pipes
    
4.2    Lateral Design
    
4.3    Sub-main Design
    
4.4    Design Charts
    
4.5    Main Line Design
    
4.6    Farm Drip System Design Examples
    Questions & Problems
        
References

5.1    Introduction
       5.1.1  Types of Distributors
    Questions and Problems
    
References

6.1    Introduction
    
6.2    Fertilizers in Drip Fertigation
    
6.3    Drip Fertigation Systems
    
6.4    Rate of Fertilizer Application
    Questions and Problems
        
References

7.1    Drip Network
    
7.2    Performance of the Drip System
        
Questions and Problems
        
References

8.1    History of Sprinkler Irrigation
    
8.2    Advantage and Limitations of Sprinkler Irrigation
    
8.3    Scope of Sprinkler Irrigation in India
    
8.4    Type of Sprinkler System and Components
    
8.5    Design of Sprinkler Irrigation System
        
Questions and Problems
        
References

1.1    General Circulation of the Atmosphere
    
1.2    World Climates
    
1.3    World Climate Classification by Köppen’s method
    
1.4    Climatic Classification over India
    
1.5    Asian Summer Monsoon and Rainfall Distribution
    
1.6    The Indian Monsoon
    Features of monsoon
    Monsoon circulation
    Onset of monsoon
    Withdrawal of monsoon
    Southwest monsoon rainfall
    Active phase of monsoon
    Break monsoon conditions
    Winter monsoon

1.7    Causes for Failure of Monsoon
    
    Prediction of onset of monsoon rainfall

1.8    Spatial Distribution of Weather Parameters over India
    Rainfall
    Temperature
    Humidity
    Sunshine and Radiation
    Wind speed

1.9    Famines in India
    
1.10    Historical Evidences of Droughts
    
1.11    Distribution of Drought Prone Regions over the Globe
    1.11.1    Occurance of drought in different countries
    
1.12    Distribution of Drought Prone Regions in India
    
1.13    Weather Phenomena Associated with Droughts
    
1.14    Effect of Droughts on Natural Resources
    1.14.1    Surface water resources
    1.14.2    Groundwater sources
    
1.15    Impact of Latest Droughts on Crop Production in India
    
1.16    Economic Impacts of Droughts in India
    
1.17    Economic Impacts of Droughts in other Nations
        
References

2.1    Definition of Droughts
    
2.2    Types of Droughts
    2.2.1    Meteorological Droughts
    2.2.2    Agricultural Droughts
    2.2.3    Hydrological Droughts
    2.2.4    Socio-economic Droughts
    
2.3    Criteria for Classification of Droughts
    Meteorological Droughts
    Agricultural Droughts
    Hydrological Droughts
    
2.4    Data requirements in Drought Analysis
    
2.5    Drought Indices
    2.5.1    Rainfall-based Indices
    2.5.2    Climate Derivate Based Indices
    2.5.3    Remote Sensing Based Indices
    2.5.4    Processes Based Indicators
    
2.6    Assessment of Drought Prone Regions in India
    
2.7    Indicators for Declaration of  Droughts in India
    2.7.1     Rainfall Deficiency
    2.7.2    Area Under Sowing
    2.7.3    Normalized Difference Vegetation Index
    
2.8    Drought Management Procedures in India
    
2.9    Drought Code adopted by Local Governments
    
2.10    Spatial Variability of Droughts and their Frequency of Occurrence in India
    
2.11    Drought Vulnerability Assessment
    
2.12    Vulnerability of Society to Drought
        
References

3.1    Factors Contributing to Climate Change
    3.1.1    Natural Causes to Climate Change
    3.1.2    Human Causes to Climate Change
    
3.2    Climatic Trends
    3.2.1    Global level
    3.2.2    National level
    
3.3    Climate Projections
    3.3.1    Global level Projections on Future Changes in Climate
    3.3.2    Climate Projections at the National level
    3.3.3    Climate Projections at the Regional level
    3.3.4    Projections of Extremes in Rainfall and Temperature
    
3.4    Consequences of Climate Change on Droughts and Agriculture
    3.4.1    Impact of Climate Change on Agriculture in some States of India
    
References

4.1    Approaches for Drought Monitoring
    
4.2    Weather Network System in Different Countries
    
4.3    Drought Monitoring using Climatological, Hydrological and Combination Approaches in Different Countries
    
4.4    International Cooperation in Drought Monitoring
    
4.5    Drought Monitoring Mechanism in India
    
4.6    Early Warning and Decision Support System
    4.6.1    Software for Drought Detection, Monitoring and Early Warning
    4.6.2    Decision Support System for Droughts Developed Outside India
    
4.7    GIS and Drought Monitoring
        
References

5.1    Global Food Production
    5.1.1    Area Under Cultivation
    5.1.2    World Food Production
    
5.2    Variability in Global and Indian Food Grain Production
    
5.3    Impact of Phenomenal Drought During 2009 on Food Grain Production in Different Countries
    
5.4    Impacts on Livestock and Fisheries
    5.4.1    Livestock
    5.4.2       Milk Production
    5.4.3    Fisheries
    
5.5    Estimation of Food Grain Production in India using Rainfall indices
    5.5.1    Trend in the annual food grain production
    5.5.2    De-trended production index
    5.5.3    Construction of rainfall indices (RI)
    5.5.4    Development of model for estimation of production index
    5.5.5    Verification of model performance
    5.5.6    Estimation of Kharif Price Production
    
5.6    Forecasting of Agricultural Output Using Space, Agrometeorology and Land based Observation (FASAL)
        
References

6.1    Hydrological Cycle
    
6.2    Global Surface Waters
    
6.3    Surface Water over India
    
6.4    Ground Water Potentials in India
    
6.5    Influence of Drought on Water Resources, its Fauna and Flora
    6.5.1    Effects on Fauna and Flora
    
6.6    Influence of Changing Climate on Droughts and Water Resources
    6.6.1    Global warming and droughts at global scale
    6.6.2    Global warming on water resources at global scale
    6.6.3    Global warming and droughts at national scale in India
    6.6.4    Global warming impacts on water resources at National scale
        
References

Good Practices for Drought Management
    7.1    Drought Planning and Preparedness
    7.1.1    Drought Planning in India
    7.2    Global Experiences in Drought Planning
    7.2.1    USA
    7.2.2    South Africa
    7.2.3    African Nations
    7.2.4    Namibia
    7.2.5    Ethiopia
    7.2.6    Botswana
Drought management plans in Europe
    7.2.7    Spain
    7.2.8    England and Wales
    7.2.9    Portugal
    7.2.10    Australia
    7.2.11    China
    7.2.12    Palestine
    7.2.13    Global Initiatives
    7.3    Drought Preparedness Action Plans in India
    7.3.1    Drought Preparedness Checklist
    7.4    Drought Preparedness Action Plans in Different Countries
    7.4.1    Arizona State in USA
    7.4.2    Texas State in USA
    7.4.3    Italy
    7.4.4    Mediterranean Region
    7.4.5    Pakistan
    7.4.6    Darfur in Sudan
    7.4.7    Bangladesh
    7.5    Indigenous Knowledge for Drought Management
    7.5.1    Astro Meteorological Methods
    7.5.2    Traditional Knowledge in Weather Prediction
     7.5.3    Traditional wisdom in Drought Risk Region (DRR)
        Rajasthan and Bundelkhand region in Uttar Pradesh
    7.5.4    Indigenous technologies for moisture conservation 
        in dry lands of semi-arid regions of India
    7.6    Traditional Wisdom for Drought Management in a few selected countries
    7.7    Role of Weather-based Agro-advisories
    7.7.1    Strategies for dissemination of advisories
    7.7.2    Role of ICAR and AICRPAM on Agromet Services
    7.7.3    Agromet Advisory services through mobile phone
    7.7.4    Status of Agromet Advisory Services in other Countries
    7.8    Value Addition to Agromet Information
    7.8.1    AICRP on Agrometeorology
    7.8.2    Thumb rules for weather-pest/diseases
    7.9    Contingency Crop Planning for Minimizing Risks
    7.9.1    Contingency Crop Planning for Rainfed Regions of India
    7.10    Drought Proofing Mechanisms
    7.10.1    Watershed Development for Drought Management
    7.10.2    Watershed Development in India
    7.10.3    The Success Stories of Watershed as a Drought Proofing Mechanism
    7.10.4    Watershed Policies and Recommendations
    7.10.5    Farm Ponds for Drought Management
    7.10.6    Success Stories of Farm Ponds
    7.11    Rainfed Technologies for the Drought Management in different agro climatic region
    7.12    Farmer’s Awareness on Climate Management
    7.12.1    India
    7.12.2    In other Countries
    7.13    Role of ICT in Climate Risk Management through AAS
    7.13.1    India
    7.13.2    Application of ICT in Providing Advisories in other Countries
    7.14    Integrated Drought Management Strategies through Farming Systems Approach
    7.14.1    Drought Adaptation Strategies in Rajasthan and in Maharashtra
    7.14.2    Improvement and Rural Livelihood
    7.15    Drought Mitigation Measures
    7.15.1    Drought Mitigation Plans
    7.15.2    Soil water Conservation and Recharging of Ground water Methods
    7.15.3    Traditional Water Harvesting and Conservation 
        Techniques
        References

Review of the state of art of Drought on impact assessment, monitoring and management with special reference to agricultural production has revealed that Drought is still a complex natural disaster and difficult to forewarn in spite of advancements in atmospheric sciences and computational procedures. It is realized only after it is set in and is not known when it is likely to withdraw. A long way one has to travel to predict its occurrence, spread and its continuation to the next season over a region. The existing net work of observational set up in developing countries in Africa, Asia and in South America need to be strengthened further for effective monitoring of significant weather events at regional and micro regional level. With the rapid expansion of communication system over the past two decades, establishment of Automatic Weather Stations (AWS) at as many locations as possible even in remote regions should be taken up on priority basis and funding may be provided by the respective governments or by the International Organizations where ever necessary. Nations with limited resources may also be convinced to strengthen their weather observational net work in view of the increased occurrences of climate extremes and changing climate. Drought vulnerability regions over the globe were delineated based on sparse historical weather data which do not match with real surface conditions. Now, with the availability of weather data from more number of stations both temporally and spatially, drought prone regions over the world may be re-delineated. This would help the planners to allocate funds rationally to the affected regions. Globally every year, some regions or others may be experiencing droughts and more so in drought prone regions.

The aim of this book is to further strengthen the knowledge of readers on management of disasters so that they are better prepared to cope with more frequently occurring natural and manmade disasters.

The process that helps us to face disasters effectively is commonly known as disaster preparedness.  When a disaster strikes, it affects us directly and immediately. While as the government, international agencies such as United Nations, Red Cross Society, non government organizations, like Ramakrishna Mission, OXFAM, CARE, resident welfare associations in urban areas and panchayats in rural areas assist us when faced with disasters the community, invariably remains the first responders. Hence, the individuals as a part of community should prepare adequately to prevent, face and respond to disasters effectively. History has  shown that where communities have prepared adequately to confront disasters, losses to life and property have been less and environment could be protected. People living in an area may be prone to more than one type of disaster. For example Kutch District of Gujarat is vulnerable to floods, drought, cyclones and to earthquakes. Such an area is called ‘multiple hazard’ zone.

The United Nations had declared the decade of 90s (1990-1999) as the International Decade for National Disaster Risk Reduction to enable developing countries to build up their capacity to cope up with disasters. The Government of India also set up a High Powered Committee (HPC), in August 1999, to review the preparedness and mitigation of natural and manmade disasters and recommend measures for strengthening the organisational structures and a comprehensive model plan for management of disasters at national, state and district level. The HPC after due deliberatons submitted the report in Feb. 2001. The HPC identified the disasters under the following types (categories) :

Appendix-'A'    :    Standard Operating Procedure for Administration
    
Appendix-'B'    :    Standard Operating Procedure for Individuals
    
Case Study    :    Assam Floods 2004
        :    Peerchu Lake - A Disaster

Introduction

Drought is temporary reduction in water or moisture availability significantly below the normal or expected amount for a specific period. This condition occurs either due to inadequacy of rainfall or lack of irrigation facilities, unexploitation or deficient availability for meeting the normal crop requirement in the context of the agro-climatic conditions prevailing in any particular area. This has been scientifically computed as Moisture Index (MI). Drought in this context can be defined as adverse MI or adverse water balance which may be attributable not only to a prolonged dry spell due to lack or sufficient rainfall but also due to such other factors as excessive evapo-transpiration losses, high temperature, low soil holding etc.

Appendix-'C'    :    Damage Prevention and Rehabilitation by Retrofitting
    
Appendix-'D'    :    Dos and Don'ts While Constructing Buildings
    
Case Study    :    Bhuj Earthquake 26th January, 2001
            Eyjafjallajoekull (Iceland) Volcano - 2010

Introduction

Landslides are simply defined as mass movement of rock, debris or earth down a slope and have come to include broad range of motions whereby falling, sliding and flowing under the influence of gravity dislodges earth material. They often take place in conjunction with earthquakes, floods and volcanoes. At times, prolonged rainfall cause heavy landslides, that block the flow of river for quite sometime.

Case Study    :    Orissa Super Cyclone 1999