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A laboratory is essential for research work in science and development. Most of the research institutes have dedicated laboratories for researchers and students to conduct experiments, perform research work, as well as to provide hands-on learning/training to students. There are certain rules and regulations laid down by the laboratory in-charge which need to be followed strictly for safety purposes. Some of the laboratories keep ‘safety manual’ for researchers to read before entering into the laboratory to conduct experiments. Whenever safety is compromised in a laboratory, there is increase in risk of unwanted incidents in the laboratory. Storing food/beverages in the same refrigerator where laboratory chemicals are kept may lead to disastrous effects on health. This chapter aims to provide instructions in brief on the things to be performed (Do’s) before and after entering onto a laboratory as well as the things never to be done (Don’ts) in a laboratory for the safety reasons.

Good Laboratory Practices (Do’s)

• P Wear apron/labcoat while working in the laboratory.
• P Use gloves/respiratory mask/fumehood while handling toxic/volatile/inflammable chemicals/reagents.
• P Use pippet or pippetor for safety in transfer of solutions.
• P Protect eyes, face and naked parts of the body from UV radiations. Use safety goggles or face mask/apron.
• P Keep your eyes away from Liquid Nitrogen and use cryogloves while handling it.
• P Arrange all the necessary materials/solutions before starting the experiment.
• P Periodically fumigate/ozonize culture and inoculation rooms.
• P Autoclave all contaminated materials before discarding.
• P Pre-treat gel or solution containing Ethidium Bromide (EtBr) before discarding into laboratory waste-disposal bin, and use gloves while handling them.
• P Burn all the transgenic plant materials in an incinerator after taking necessary observations.
• P Make entry in the designated log-book and clean the instrument after using it properly.
• P Switch-off the instrument after use or when not in use.
• P Wash hands with soap and remove the labcoat before leaving/going out of the laboratory7.
• P Ensure that switches for fans/lights, and water taps are off while leaving the laboratory.
• P Follow the biosafety guidelines suggested for handling and disposal of the hazardous chemical.

Introduction

Resources must be managed effectively to achieve maximum sustainable agricultural production from limited natural resources. Many of the resources that agriculture depends on (such as water and land) are finite and need to be well managed for long term sustainability. In most cases, this means avoiding adverse effects on the society and environment. With the development of agricultural sectors, the utilization patterns of natural resources in agriculture are important concern for policy makers in present climate change scenario. Unlike other production activities, agricultural activities also utilize factor resources to produce several marketable products or services. The way the natural resources are utilized in agriculture and its concern for society and environment, plays a vital role in economic viability and ecological sustainability of agriculture.

There are several conventional methodologies to estimate the level of profit and efficiency of production system at enterprise level. Only marketable factors are considered in conventional methods keeping the non-marketable component of production system away. Non-marketable component of a production system includes various social and environmental dimensions of it. Nammalwar (1997) has explained the concept of environmentally sound and sustainable enterprises, which include the physical resources, ecological resources, human use value, quality of life values with either way impact of agriculture and environment. In recent years, non-marketable dimensions of agricultural activities have been given importance because of its concern for the environment and society. This chapter provides a methodology that incorporates non-marketable component of agricultural enterprise besides its marketable component. The Resource Cost Ratio (RCR) approach is a modified methodology developed by Debnath et al. (2010) from Domestic Resource Cost Ratio (DRCR) that Morris (1990) has applied at macro-economic level to determine the comparative advantage between countries.

Introduction

The influence of various socio-economic factors on the willingness of the decision makers to adopt new technologies has been investigated by a number of studies (Roe, 1983; Shakya and Flinn, 1985; Thomas et al., 1990). In most studies of adoption behaviour, the dependent variable is constrained to lie between 0 and 1 and the models used are exponential functions (Kebede et al., 1990). However, the decision to adopt a new technology can be very effectively captured using binary choice models. Binary choice models are appropriate when the choice between two alternatives depends on the characteristics of the problem. Application of a linear probability model to this type of problem, however, suffers from a number of deficiencies (Capps and Kramer, 1985), particularly, the one associated with the estimated probabilities in some cases being greater than one or lesser than zero as a result of neglecting significant interaction effects (Mingche, 1977). These deficiencies could be circumvented through the use of a monotonic transformation (probit or logit specification) which guaranties that predictions lie within the unit interval (Capps and Kramer, 1985). Univariate logit and probit models and their modified forms have been used extensively to study the adoption behaviour of farmers and consumers (Schmidt and Strauss, 1975; Garcia et al., 1983; Shakya and Flinn, 1985; Harper et al., 1990). According to Hanushek and Jackson (1977), the choice between logit and probit models is largely a matter of convenience. However, Maddala (1983) and Shakya and Flinn (1985) have recommended probit models for functional forms with limited dependent variables that are continuous between 0 and 1, and logit models for discrete dependent variables.

Model Specification

Univariate logit model is generally used to study the adoption behaviour of farmers and consumers.

The model is estimated using the maximum likelihood method.

Parametric and Non-parametric tests

Parametric and Non-parametric tests are used for comparing differences between groups (e.g. Difference in catch-per-unit-effort (CPUE) of two types of Codends) and to test the relationship between two or more variables (e.g. Gender differences in awareness levels of fish farming (High, medium and low). As there seen differences in types of data, Non-parametric statistics are used when

• Data is non-normal

• Small sample size

• The variables are categorical

• Short response categories (<5 ) in scales

Introduction

Precise learning of the components of the general structure of a research article is inevitable in writing a research article. The general structure of a research article consists of Title, Abstract, Keywords, Introduction, Methods, Results and Discussion, Conclusion, Acknowledgements, References and Supporting materials. There are no specific guidelines for which one of the above should be written first.

Process of Writing - Building the Article

To start writing a research article, it is not advisable to consider all the tables and data that are available to the writer. The easiest way to start with is to choose the tables and data that can be used wisely in the paper. The writer should be aware of the types of the chart or table which are accepted and the number of charts and tables that should be included in the paper (generally a research paper contain 7 to 9 figures and charts in each category). It is also not recommended to use conventional ways of presenting data and figures (like bar charts, pie diagram). The figures or illustrations should not be so simple. Figures and Tables should be self-communicating. The reader of the paper is very well educated and may have mastery in the particular subject. The language and illustration should be appealing to them. The figures and illustrations should have an impact on the reader to think and draw inference from the data provided by the research. Two dimensional and three dimensional data presentation create more interest in the reader rather than the one dimensional presentations. Next to consider is Methods that explain how the data was collected. In case of writing the methods in research paper, unlike thesis, the methods need not be elaborated in the paper. Results are the description of the data and the broad observation made. Results should not contain the inferences that are already known. Results should be supplementing the tables and charts, not a description of them. Discussion involves the comparison of the obtained result and the previous results. The contribution to the previous research results can be discussed. New findings and solutions can also be discussed. Conclusion is a single paragraph which altogether summarizes the final outcome. Generally the conclusion should not contain citations.

Introduction

The gut microbiota is the complex and dynamic community of microorganisms that includes various bacteria, fungi and other microorganisms, proliferate and lead a symbiotic/mutual relationship with the host system. These microorganisms protect the host against pathogens by unique mechanisms, helps to maintain shape and strengthen the intestinal epithelium and boost immune response. Diverse bacterial metabolites can immigrate the host bloodstream and other parts of the body via various channels in humans and animals, such as gut-microbiota-brain, gut microbiota-skin, gut-vagina, gut liver, gut-bones etc. However, many aspects of modern lifestyle would alter the gut microbiota and lead to numerous diseases including inflammatory bowel disease, diabetes mellitus, obesity, and metabolic syndrome. Probiotics and other beneficial microbes exert many beneficial effects on their hosts by modulating the gut microbiota. While the term probiotic is reserved only for well-characterised strains with clinically proven health benefits, other beneficial microorganisms include, organisms responsible for the fermentation of foods viz. yogurt, kefir, kombucha, kimchi and many others that have been shown to have distinct health benefits. Interestingly, major group of these beneficial organisms belong to lactic acid bacteria.

The process of making dried milk involves eliminating water from milk, primarily through spray or roller drying. It is regarded as a significant sector of the dairy industry and is made from whole cow, buffalo, or standardized milk. Skim milk powder (SMP) is made from milk after the fat has been removed whereas whole milk powder (WMP) is made from whole cow or buffalo milk

FSSA (2006) Standard Of Milk Powder According to FSSA (2006), Milk powder is the product obtained by partial removal of water from the milk of cows and/or buffalo. The fat and/or protein content of the milk may be adjusted by the addition and/or withdrawal of milk constituents in such a way as not to alter the whey protein to casein ratio of the milk. It shall be of uniform color and shall have pleasant taste and flavor, free from off-flavor and rancidity. It shall also be free from vegetable oil/fat, mineral oil, thickening agents, added flavor, and sweetening agents. It may contain food additives. It shall conform to the microbiological requirements prescribed by FSSA (2006).

Water activity, high storage temperatures, longer than usual storage times, high product moisture content, high processing temperatures, and container headspace are some of the variables that affect the Maillard reaction in dried milk.

Casein and whey proteins are the major proteins present in milk. Unlike casein, whey proteins are heat-labile. Whey protein fractions present in milk are α-lactalbumin, β-lactoglobulin, bovine serum albumin, immunoglobulin, euglobulin, and lactoferrin. β-lactoglobulin and κ-casein interact during heat treatment. When milk is refrigerated for longer than 24 h, it forms γ-casein and protease-peptone fractions.

One of the most consumed and well- known milk products in the Indian subcontinent is ghee. It is a by- product of tradition, prepared both at organized dairy plants and the household level for a well- established market. Ghee is traditionally made from the milk of cows or buffalo. The tetrapyrrole pigments biliverdin and bilirubin are responsible for the distinctive color of buffalo ghee. On the other hand, ghee is golden yellow due to the presence of carotenoids in cow’s milk. Ghee’s composition changes according to the kind and feeding habits of the animal and can differ from place to place and season to season.

In addition to, being a substantial source of fat- soluble vitamins, essential fatty acids, and other growth- promoting components, ghee is a rich source of energy. Its value as food for humans is enormous. Additional attributes related to medicine are present in ghee. Ghee can improve one’s physical and mental attributes and treat eye conditions like ulcers. Ghee is used in religious and ceremonial rites in addition to being consumed.

Composition

Ghee is a complex lipid composed of mixed glyceride which makes up 99–99.5% of its total content. In addition, it has trace amounts of calcium, magnesium, phosphorus, iron, copper, and fat- soluble vitamins, tocopherols, carbonyls, hydrocarbons, carotenoids, free fatty acids, phospholipid, sterols, and sterol esters

The terms “cultured dairy foods,” “cultured dairy products,” and “cultured milk products” are other terms for fermented dairy products. Lactobacillus, Lactococcus, Leuconostoc, and other lactic acid bacteria, along with yeasts in certain products, are used to ferment milk to make these dairy products. The product’s shelf life is extended through fermentation, which also improves the product’s taste and milk’s digestibility. There is a wide variety of fermented milk products available, each with unique flavors and attributes. The following categories apply to fermented milk products, based on the type of fermentation.

Changes in Nutrients Resulting from Fermentation

Fermentation lowers the lactose content in fermented milk products; however, if the starting milk is concentrated or fortified, the finished product’s lactose content may be higher than that of liquid milk.

The composition and content of protein and total amino acids are comparable to those of raw milk. Casein degradation is small compared to cheese, accounting for not more than 1% of the total protein. While some products of casein degradation are used by the starter microorganisms, others build up as free amino acids in the fermented kinds of milk. The amounts of free amino acids that build up differ depending on the species. Generally, proline is increased in large quantities, followed by serine, alanine, valine, leucine, and histidine. As a result of continuous starter culture activity, the level of free amino acids increases during storage. A limited degree of lipolysis occurs during the manufacture of fermented milk. The amount varies depending on the type of species, but the pattern and concentration of free fatty acids are both impacted. During fermentation and the breakdown of amino acids, trace amounts of volatile fatty acids are also produced.

Things required for cell culture

1. Lab – having negative pressure (HEPA filter)

2. Safety cabinet (Hood/laminar flow)

a) Biosafety level 1 (BSL-1) – Basic level of protection. Agents handled under this cabinet are NOT infectious.

b) BSL-2 – Appropriate for moderate risk agents. Most of the non infectious cell cultures are done under this facility.

Cell counting is the fundamental techniques in various fields of biology, medicine and biotechnology.

Importance of cell counting

1. Research: Cell counting is a critical component of biological and medical research. It helps scientists understand the behavior of cells in various contexts, such as studying cell proliferation, differentiation, and response to stimuli.

2. Disease diagnosis: Monitoring various medical conditions, including infections, autoimmune diseases, cancer and blood disorders. Complete blood counts (CBCs) are routinely used to assess the levels of different blood cell types and assess disease conditions.

3. Stem Cell Research: Cell counting plays a significant role in stem cell research, where precise quantification of stem cells is necessary for various applications, including regenerative medicine and tissue engineering.

4. Cell Culture: Cell counting is integral part to determine seeding density. Seeding of cells to available surface area for growth is important consideration for successful culture.

5. Cell-Based Assay: The cell-based assays are used for drug screening, toxicity testing, and evaluating the efficacy of new compounds. Accurate cell counts are essential to ensure the reliability of assay results.

Introduction

Peripheral blood mononuclear cells (PBMCs) consist of chiefly of lymphocytes and monocytes. Purified PBMCs are used in vitro to evaluate a variety of functions of lymphocytes viz; a) proliferation to mitogenic (PHA, Con-A) stimulation, b) monitoring of prior sensitization in antigen recall assays by scoring lymphocyte proliferation, c) immunophenotyping for surface markers as well as intracellular molecules in monocytes and lymphocytes etc. Activation of monocytes/macrophages by small molecules, cytokines and pathogen components can also be monitored. PBMCs can also be used for a variety of structural and functional studies for addressing issues in human immunology such as scoring for apoptosis and production of cytokines as well as other mediators in vitro.

Background information

Blood is a liquid mixture comprised of plasma, cells and proteins. The liquid portion, plasma, makes up half of the blood volume, and cells the other half. Isolation of lymphocytes is a common procedure in immunology and cell biology that involves separating and collecting lymphocytes.

Principle

The quantification of infectious virus particles is commonly achieved through the utilisation of the Median Tissue Culture Infectious Dose (TCID50) assay. The assay functions through the introduction of a serial dilution of the virus sample into cells in a 96 well plate format. The chosen cell type is specifically intended to exhibit a cytopathic effect (CPE), which refers to observable changes in morphology or cell death resulting from viral infection. Following a period of incubation, the cells are examined for cytopathic effects or cell death, and each well is categorised as either infected or uninfected. The concentration at which 50% of the wells exhibit a cytopathic effect (CPE) is employed to determine the 50% tissue culture infectious dose (TCID50 ) of the viral sample. The end point is the dose or dilution that infects or kills or shows CPE of the cells. The calculation can be performed using various mathematical techniques, such as the Spearman-Karber method or the Reed Muench method. The virus titer is quantified as the number of tissue culture infectious dose 50 (TCID50 ) per millilitre (ml). The non-cytopathic virus can be quantified by employing the fluorescent antibody test, immune-peroxidase assay, or other appropriate methodologies.

Principle

Cell viability refers to the quantity of healthy cells in a sample, while cell proliferation is a crucial indicator for comprehending the mechanisms and pathways involved in the survival of cells or death following exposure to toxic substances. The methods used to assess viability are typically the same as those used to detect cell proliferation. Cell cytotoxicity and proliferation assays are commonly employed in drug evaluations to determine if the test compounds impact cell proliferation or exhibit direct cytotoxic effects. Cell viability can be determined by calculating the ratio of total live cells to the total number of cells (live and dead). Staining also helps to observe the general cell structure. Cell proliferation assay by MTT. Metabolically active cells reduce the yellow tetrazolium MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) through dehydrogenase enzymes to produce NADH and NADPH. The purple formazan produced inside the cell can be dissolved and measured using spectrophotometry. The MTT Cell Proliferation Assay quantifies cell proliferation rate and detects decreases in cell viability caused by metabolic events leading to apoptosis or necrosis. The MTT Reagent produces minimal absorbance values when no cells are present.

The affections of abdominal cavity incidence wise are on the top followed by thoracic cavity. Both these affections lead to high morbidity and heavy mortality losses. Most thoracic affections produce almost similar clinical signs but abdominal affections can show different clinical signs. Radiography and ultrasonography play an important role in differentiation and confirmation of various affections.

Radiography is an important diagnostic tool for both thoracic and abdominal diseases in small animals but for large animals it is more useful for thoracic affections as compared to abdominal affections. In large animals, the use of radiography is limited up to the ventral aspect of the abdomen only i.e. for diagnosis of diseases of reticulum, diaphragm and sternebrae. In thoracic cavity, there are numerous diseases that need to be differentiated. Pneumonia, lung cyst, lung abscesses, pleuritis and pleural effusions are some important diseases apart from presence of foreign bodies. In such a situation, radiography can play an important role as an aid in the diagnosis of various affections.

Similarly ultrasonography has also played a major role in the diagnosis of abdominal diseases in small animals such as dogs and newborn calves especially in the field of G I tract and urinary system. The ultrasonography is advantageous over radiography in the sense that internal anatomy of the soft organs can be better known and studied by ultrasonography as compared to radiography. It is also considered to be extremely safe for both the patient and the operator.

General Instructions

The monitor of ultrasound machine should be placed at the eye-level. The daylight should not be too bright. The setting of the machine should be done to obtain best picture on the screen. There are various knobs on the machine to set contrast and brightness. There are three other knobs that are used to set near gain, far gain and overall gain. There is also a brightness bar (help-bar) on the screen with different brightness areas. These knobs should be turned clockwise or anti-clockwise, so that all the areas on the help bar (brightness bar) are visible.

Transrectal Procedure

In cattle, trans-rectal ultrasonography is done in a way, similar to rectal examination done for palpation of genital organs. Following steps are suggested for proper procedure

1. Properly restrain the animal.

2. Evacuate the feces from the rectum.

3. Select a transducer of proper frequency (the linear array transducer of 5.0 MHz frequency is useful for general purpose in bovines, however for detailed examination of early conceptus and small follicles, a transducer of 7.5 MHz can also be used. Some transducers have range of these frequencies in single-equipment. Such transducers of switch-able frequency are found more useful.

Diagnostic techniques are routinely used for diagnosing different surgical conditions. The most frequently used imaging techniques in veterinary practice are radiography and ultrasonography. Radiography and ultrasonography are not suitable for each organ in large animals. In small animals most organs are suited to ultrasound images except bones and gascontaining structures such as lungs etc. Bone and gas reflect ultrasound and do not allow the sound waves to form image therefore radiography gives better diagnostic images for these organs. The liver, spleen, kidneys, prostate, gravid uterus and fluid containing organs like urinary bladder, gall bladder and major blood vessels can be better imaged with ultrasound. In buffaloes in surgical section important clinical conditions relate to urinary and digestive systems, mammary gland and perineal area.

1. Urinary System: In bovine, the cases of obstructive urolithiasis are commonly observed. These types of cases are more common in bullocks and in buffalo calves. In these cases radiography is not so useful. Sonography can be tried to take the image of ruptured urethra in bullock and can be compared with radiography. Due to ruptured urethra there will be accumulation of urine in the s/c tissue of prepuce. Due to fluid density the image will be radioopaque and will appear white and no detail of the organ can be seen. The ultrasonogram (USG) will show images of glans penis and its coverings. Different diameters of urethra and a point of rupture just behind to glans penis can be make out. Due to obstruction, The B.calves either will pass drop wise urine or there will be complete stoppage of the urine. These cases can also be easily diagnosed by ultrasonography.

Ultrasonographic images of stomach are required to diagnose ingestion of any abanormal materials by dog. When content of stomach are dry or there is gas in stomach then, it is difficult to get proper image of stomach. Offering water to animal or oral administration of water with the help of syring is also helpful in getting the images of content of stomach. Some time, while doing ultrasonography, movement of contents of stomach can be visualized. Stomach can be images between liver and spleen. The size depends upon contents or intake of food by dog. For proper image of gastrointestinal tract including stomach, there should not be gas in GIT. One should remember that ultrasound beam does not travel in gas. The best medium of ultrasound beam is liquid.

Scanning Technique

If possible, fasting for 12 hours is good to diminish the amount of gastrointestinal gas. However, non-fasted dogs, when offered water may show adequate image quality. The water may be administered in quantity of 15 ml per kg of bodyweight. Removing the stomach gas using an orogastric tube has been recommended tto enhance the visualization of lesions of the stomach, however, this is not in practice in India.

Ultrasonography in bull is helpful in diagnosis of problems of testis and accessory glands. This can also be used in assessment of development of these organs. Such developmental assessment, when combined with endocrine data, is useful to know age of puberty, sexual development and dimension of these organs. The ultrasonographic images and data are available for testis, bulbourethral gland, prostate and seminal vesicles in the bull. Ultrasonography of testis of bull can be useful to assess developmental changes, testicular dimensions and pathological conditions of the testis viz. tumors, lesions, abscess etc. Epididymis can also be examined for change in lumen, inflammation, granuloma etc. The accessory sex glands can be better viewed in ultrasonographic scanning to assess their dimensions, normal developmental changes and alterations in their morphology. Before, ultrasonography is undertaken, one should thoroughly revise the knowledge of sectional anatomy and location of these organs.

Testis

Location: The testis, in the bull, is located in the scrotum. In abnormal conditions, the testis might be retained in the inguinal canal or abdomen. Before, ultrasonography, location of the testis should be assured. For this, the testis should be palpated to assess their pliability and rule out adhesions with the scrotal wall. In normal condition, the testis should be freely movable in the scrotum, soft of palpation and both the testis should touch the bottom end of scrotum.

Mammary glands are important part of body which synthesize and store the milk in between milkings or suckling by the calf. Therefore it attains prime importance in any dairy animal. However, it has received little attention in buffaloes till now. Buffalo presents several unique characteristics in terms of milk production, length and shape of teats and arrangement of duct system in the mammary glands. Development of udder may be related to milk production and the knowledge of changes in its structure in relation to different physiological stages may also have practical implication.

Transducer Selection and Preparation of Scanning

Preparation of the site to be imaged will enhance the quality of the image. Therefore, any loose hair and dirt must be clean off. Ultrasound gel is applied to the area of interest. The gel acts as a coupling agent to prevent air from trapping between the transducer and skin. A 7.5 MHz transducer is ideal for imaging the teats. A 5.0 MHz transducer provides an adequate image. The mammary gland should be scanned with a 5.0-7.5 MHz transducer to give adequate penetration. All structures should be scanned in cross section and longitudinal planes.

Veterinary ultrasonographer has to write report of ultrasonography in different animals. The proper ultrasound report writing is the moral duty of the vet ultrasonographer. The accuracy of diagnosis must be ensured. The poor resolution/quality of machine may lead to erroneous diagnosis. Therefore, veterinarian must ensure good quality of machine and proper diagnosis. The report might be different in different animals e.g. report writing about uterus will be different in bitch, cows, buffaloes, camel, horse, goat and sheep. However following might be guidelines in writing ultrasound report of uterus. fetus, endometrium

Uterus: Position of uterus, contents in the uterus, shape and size in initial pregnancy and infections, appearance of myometrium, thickening of uterine layers, presence or absence of fluied/pus/masses, left and right horns and their contents. Observations on the images of uterine fluid and its echoicity, membrane appearance in pregnant animals. Special attentions should be paid to endometrium.

Endometrium: Appearance and thickness; any growth on the surface of endometrium. Images of retained portion of placenta can be ascribed in the cases of retained placenta.

Ovary: Ovaries are scanned to assess cyclic structures, size, shape, its Position/ location and internal echo pattern of follicles. This echo pattern will indicate whether follicle is normal, cystic or with any other abnormalities of follclular f luid. Ultrasound reports also include shape and size of corpus luteum. In cows and buffaloes, presence of cavity, looseness/compactness of luteal cells are also mentioned.

Cell culture refers to removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been establwashed.

Primary culture refers to the stage of culture after the cells are isolated from tissue and proliferated under appropriate conditions until they occupy all of the available substrate (i.e., reach confluence). At this stage, the cells have to be subcultured (i.e passaged) by transferring them to a new vessel with fresh growth medium to provide more room for continued growth.

Pharmaceutical and chemical industries depend largely on toxicological evaluations to provide information regarding safety of their commercial products. Toxicologists so far have been relying on animal studies to investigate potentially beneficial or harmful effects of drugs and chemicals prior to testing them in humans. Traditionally, the safety assessment process during drug development has been based on screening indiscriminately a plethora of new chemical entities (NCEs) using large number of animals as testing models in order to identify new therapeutic agents with the expectation that one or a few of these NCEs have some competitive advantages over the existing therapeutic agents. That is why they are more efficacious, bioavailable, selective, and safer. This approach is costly, time-consuming, and requires large quantities of test material. Currently, it takes about 10-12 years and a huge expenditure for a drug to move from research laboratory to patent. Thus alternative cell culture models in the early phases of drug discovery process can save time and money both by eliminating least beneficial NCEs.

Apoptosis is triggered by extrinsic and intrinsic stimuli as radiation, oxidative stress and genotoxic chemicals. It is defined by characteristic changes in the nucleus morphology including chromatin condensation and fragmentation, overall cell shrinkage/rounding and formation of apoptotic cell bodies. Among the many markers of apoptosis, DNA fragmentation is considered a hallmark. The DNA ladder assay described here is a simple, sensitive, cost-effective and rapid method for estimating apoptosis in cells.

Isolation of Genomic DNA

Genomic DNA from primary cells culture of PMNC and splenocytes of rats is isolated by using standard phenol-chloroform DNA isolation protocol of Sambrook and Russel (2001) as described below:

1. Preparation of primary cells culture of different cells, namely-PBMNCs, thymocytes and splenocytes form Wistar rats and viability checked by trypan blue dye exclusion test.

2. 106 cells/ml of primary cell culture is used and seeded in 24 well culture plates containing along with treatment with xenobiotics at different µM concentration.

3. Cell culture plates are incubated in CO2 incubator at 37°C with 5% CO2 for 12 hrs.

4. The cells are collected in 1.5 ml of micro centrifuge tube and centrifuged at 3200 rpm for 10 min at 4°C; supernatant is discarded and pellet is washed with PBS (pH 7.2).

5. DNA extraction buffer (500 µl/tube) is added in tubes. Gently tap to disperse the cell pellet in extraction buffer and keep in water bath for 1.0 hr at 37 °C (it is to ensure that pellet is completely suspended so that cells are accessible to SDS and Proteinase K).

6. 10 % SDS is added (20 µl/ml) to cell suspension and the tubes are gently mixed by inverting the tube. The contents of tube become viscous which indicates lysis of cells. After that sample is handled gently from onward to avoid shearing of DNA.

7. Proteinase K (15 µl) of 20 mg Proteinase K/ml of buffer is added in tubes in two pulse i.e. half the requirement is added to tube in 1st pulse, mixed gently end to end and kept in water bath at 50°C. After 3-4 hrs, the second pulse of the remaining amount of Proteinase K is added and tubes are incubated at 50°C overnight.

8. Equal volume of equilibrated phenol (Tris saturated phenol pH> 7.8 ) is added in the tubes next day morning. Mix the contents of tube gently by inverting the tube for 15 min till a light coffee coloured uniform solution without any balls of phenol is formed and then centrifuge at 3400 rpm for 15 min.

High-performance liquid chromatography or high-pressure liquid chromato graphy (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture. It involves the injection of a small volume of liquid sample into a tube packed with tiny particles (1.8- to 5-micron (μm) particle size called the stationary phase) where individual components of the sample are moved down the packed tube (column) with a liquid (mobile phase) forced through the column by high pressure delivered by a pump. These components are separated from one another by the column packing that involves various chemical and/or physical interactions between their molecules and the packing particles. These separated components are detected at the exit of this tube (column) by a flow-through device (detector) that measures their amount. An output from this detector is called a “liquid chromatogram”.

Aflatoxins are toxic and carcinogenic compounds produced as metabolites by many Aspergillus species. This fungus invades habitats such as soil, grain, nuts, or decaying vegetation when- ever suitable conditions such as high moisture and temperature are met. B1 is the most toxic of at least 14 different types of naturally occurring aflatoxins. Therefore, many countries and regulatory agencies impose strict limits on B1 as well as other common aflatoxins such as B2, G1, and G2 despite exhibiting lower toxicity. The U.S. FDA set the action level to20 μg/kg (ppb) for human food and to 300 μg/kg (ppb) for animal feed. Aflatoxin B1 is extremely widespread and is present in food and feed products such as cottonseed, corn, and peanuts. It is highly toxic and has been classified as a group 1 carcinogen by the WHO. The aflatoxins B2 , G1 , and G2 are usually found accompanying B1 , in lower concentrations in the contaminated samples. The order of toxicity is AFB1 > AFG1 > AFB2> AFG2.

The cytological insult due to exposure of different environmental pollutant viz. pesticides, herbicides etc. impose great loss to the vitality and health of the individuals. The basic pathophysiological distrubances following the expo sure of these deterrents to the different visceral organs leads to dysfunction of these organs. Imbalance between body’s antioxidant defense and peroxidation of the lipid layer of cells’ causing free radical generation is defined as Oxidative stress. Free radicals are the molecules or entity that contains one or more than one unpaired electron. Pathophysiology of several diseases are associated with the generation of oxidative insult and many xenobiotics associated toxicity are also a result of oxidative damage and generation of reactive oxygen species and reactive nitrogen species. Alzheimer’s Disease is one of the fine example of oxidative damage associated neuronal degeneration.

Oxidative stress can be estimated using following parameters:

1. Lipid peroxidation (Paula et al., 2005)

2. Reduced glutathione (Sedlak and Lindsay, 1968)

3. Superoxide dismutase (Madesh and Balasubramanian, 1998)

4. Catalase (Aebi, 1983)

Tissue Collection

Rats are most commonly employed as test subjects to conduct the experiments related to drug metabolizing enzyme assays involving various enzymes i.e. microsomal and non microsomal enzymes. Rats are humanely sacrificed at the end of the exposure period of 28 days of any therapeutic agents or drug and the liver of all the animals has to excise and immediately wash with ice-cold normal saline, weigh and perfuse with ice-cold 1.15% KCl solution containing 0.05 mM EDTA. Livers are processed further for preparation of microsomes for determination of microsomal drug metabolizing enzymes.

Preparation of Microsomes

Livers tissues are homogenized in 3 volumes (1:3) of ice-cold (1-4o C) homogenization buffer containing 1.15% potassium chloride in 50 mM potassium phosphate buffer solution (pH 7.4). The homogenates are centrifuged at 15000 g for 30 min (Hitachi cs120GX II). The mitochondrial supernatant (8 ml) is transferred to ultracentrifuge tubes and spun at 105000 g for 1 hour in ultracentrifuge to sediment the microsomal pellet. Cytosolic fractions are collected from the middle separating the lipid layer on top in separate micro centrifuge tubes. The microsomal pellet is then washed by adding 8 ml of resuspension buffer (homogenization buffer containing 0.05 mM EDTA) and spun again 105000 g for 15 min. The microsomal pellet so obtained is resuspended in 4 ml of resuspension buffer and 1 ml aliquots are stored immediately at -80o C until assayed for drug metabolizing enzymes.

Pharmaceutical and chemical industries depend largely on toxicological evaluations to provide information regarding safety of their commercial products. Toxicologists so far have been relying on animal studies to investigate potentially beneficial or harmful effects of drugs and chemicals prior to testing them in humans. Traditionally, the safety assessment process during drug development has been based on screening indiscriminately a plethora of new chemical entities (NCEs) using a large number of animals as testing models in order to identify new therapeutic agents with the expectation that one or a few of these NCEs have some competitive advantages over the existing therapeutic agents. That is why they are more efficacious, bioavailable, selective, and safer. This approach is costly, time-consuming, and requires large quantities of test material. Currently, it takes about 10-12 years and a huge expenditure for a drug to move from research laboratory to patent. Thus alternative cell culture models in the early phases of the drug discovery process can save time and money both by eliminating least beneficial NCEs.

Preparation of Reagents

Culture media

For the culture of hepatocytes medium M199 (GibcoBRL, USA) is used. One pouch of the dry media is dissolved in 1 liter of filtered distil water. The pH of the media is adjusted by adding 0.4% sodium bicarbonate to be around 7.4. The media is than sterilized by filtration through seitz filter (0.22 mm) and stored in 50 ml sterilized conical flasks.

Antennae are paired, highly mobile, segmented appendages present between or below or behind the compound eyes. The antennae arise from the 2nd or antennal segment of the head possessing nerves coming from deutocerebrum of the brain. Antennae are absent in order protura and class Arachnida whereas 2 pairs of antenna (antennules) are present in class Crustacea.

Antennae are set in a socket of the cranium called antennal socket (anetnnifer) and connected by an articulatory membrane which allows free movement of antenna. Antennal socket is provided with an antennal suture. Although antennae vary widely in shape and function, all of them can be divided into three basic parts:

• Scape

• Basal segment, larger than other segments and articulates with the head capsule. It is provided with intrinsic muscles.

• Pedicel

• Second segment of antennae. In this, mass of cells called Johnston organ (auditory) is present (eg. houseflies). The above two segments have intrinsic muscles ie., their own muscles. In honey bee, wasps pedicel forms the pivot between scape and flagellum.

• Flagellum/Clavola

• The remaining segments or annuli or flagellomeres are called flagellum which lack individual muscles. Flagellum is supplied with many sensory receptors that are connected by nerves to brain. Flagellum is further divided into three parts

Light spectrum holds rich information about the world. The spectrum of natural illumination changes with daily cycle and objects differ in spectral reflection or emission properties. Like many other animals, insects use chromatic information to f ind favorable habitat, to efficiently locate food sources and to identify conspecific mates using the spectral information. Pollinating insects such as many species of bees and butterflies use their color vision to maximize success in foraging. They detect flowers, memorize the colors and patterns of rewarding flowers, and preferentially collect nectars from the flowers in their later visits. Visual guidance of behaviour is challenging when photons, the elementary particles of light, are scarce. To produce a reliable representation of the surroundings, the visual system must (i) ensure absorption of a sufficient number of photons into a photoreceptor, (ii) house photoreceptors that efficiently convert each photon absorption into a neural signal, and (iii) process these signals appropriately. Insects are a numerous and diverse class of arthropods that have evolved to occupy ecological niches from the brightest to the darkest.

Introduction

Fruit crops have been cultivated for centuries, both commercially and in amateur gardens, and many species covering a wide range of trees, shrubs, and non-woody perennials.Fruit crop species have certain common characteristics: they are long-lived, usually highly heterozygous, and clonally propagated from an elite mother plant. Fruits are increasingly recognized as an important component of a healthy diet, and consumption of temperate fruit and fruit products, especially juices and dairy derivatives, has risen sharply over the past decade.China is the largest producer of fruit crops with approximately.

• India ranks the second in fruit production.

• India has witnessed increase in horticulture production over the last few years. Significant progress has been made in area expansion resulting in higher production.

• Over the last decade, the area under horticulture grew by 2.6% per annum and annual production increased by 4.8%.

• Production of fruit crops has increased from 50.9 Million Tonnes to 97.35 Million Tonnes since 2004-05 to 2017-18.

• Total production of fruits is highest in case of Andhra Pradesh followed by Maharashtra.

INTRODUCTION

Aquaculture is the fastest growing food producing sector with annual growth rate of 10.34% and it play a very important role in improving the income generation as well as nutritional security of farmers in many developing countries including India. In the fresh water system, carps are the most widely cultured species throughout the country and carp culture in India is believed to be as old as carp culture in China. Although in early years, carp culture was largely restricted to the North-Eastern States of India, during the last four to five decades, it has spread to almost all parts. The traditional carp culture practice is rapidly transforming into scientific farming in several parts of India. Generally, carp culture in India is a polyculture of 3-6 species of major carps and exotic carps (Vijay Anand, 2019), namely, catla (Catla catla), rohu (Labeo rohita), mrigal (Cirrhinus mrigala), silver carp (Hypophthalmichthys molitrix), grass carp (Ctenopharyngodon idella) and common carp (Cyprinus carpio). Among six species, common carp (Cyprinus carpio) cultured in the composite fish farming system is potential species, which plays a vital role in the Inland Fish production especially for the North Eastern Hill states of India as the species has got high adaptability and tolerance towards high range of temperature and dissolved oxygen variation. The species is introduced to India in 1937 as the Prussian strain and in 1957 as the Bangkok strain. Due to their high food value, the farmers of the state have been culturing the fish in rice fields in monoculture or along with other species particularly in Apatani Plateau of Ziro area gaining an average weight 75g to 110g (Hussain et al., 2018).

Entomology is the science, which deals with study of arthropods which parasitize or affect the domesticated animals. Since these arthropods can cause damage or injury to their host by several ways, thorough understanding of the subject is very essential. Most of these arthropods are ectoparasites living on the body of the host or at the most in subcutaneous tissues while very few are endoparasites, living inside the body of the host. Similarly, most of the arthropods are temporary parasites, while very few like lice and mites are permanent parasites. Arthropods comprise of more than 80% of living creatures on earth.

Harmful Effects Caused by Arthropods to the Body of the Host

1. Injury to the skin: Most of the arthropods have biting tendency and cause injury to the skin by bites. As a result, micro-wounds are produced on the body which may later on be infected with bacteria leading to localised or generalised pyoderma condition or pyodermatic patches. Similarly, such wounds are predisposed to attack of blow flies which cause a condition called as “Blow fly myiasis.” Animals in order to escape the attack of flies try to run all around leading to phenomenon known as “Entomophobia”.

2. Blood sucking Habits: Most of the arthropods have blood sucking tendency like mosquitoes and ticks which may lead to anaemia.

3. Release toxins: Few of the arthropods release poisonous substances while biting. They inject certain poisons and toxins in the body of host. Eg. Some species of ticks release toxins causing paralysis in host known as tick paralysis.

4. Transmission of diseases: Arthropods have ability to transmit various bacterial, viral, protozoal, helminthic and mycotic pathogens. While sucking blood, they collect infective stages of the organism and while biting to the other host, they transmit the pathogens.

Class: Insecta

It is the biggest class in animal kingdom. About 70% of species of animals are included in class insecta.

General Morphological Characters

1. All the character of arthropoda are also present in this class.

2. Body is divided into three tagmas i.e. head, thorax and abdomen.

Normally body of arthropods consists of twenty segments. Head region is formed by the fusion of 1st six segments. Thorax is formed by next three segments and abdomen formed by last 11 segments.

Modification of six pair of appendages of head: 1st pair of appendages is modified into eyes. 2nd pair is modified into antennae. 3rd pair is lost. 4th pair of appendages is modified into mandible. 5th pair modified into first maxillae and 6th pair modified into 2nd maxillae means mouth part is nothing but modification of 4th, 5th and 6th pair of appendages. On abdomen, appendages or legs are lost.

Morphology of head region of insects

Highest point of head known as “Vertex”. Anterior portion sloping in forward direction known as “Frons”. Posterior portion sloping towards thorax is “Occiput”. On the medial aspect of head, there is a pair of compound eyes. Each compound eye is made of number of lenses called as “facet”. Compound eyes may meet one another in mid line (holoptic) or are wide apart (dichoptic). Just medial to compound eyes, there is a pair of non-functioning simple eyes known as “ocellus.” Just in front of these two eyes on either side, there is a pair of antennae. Antennae are made up of three parts, base, pedicle and f ilaments. Pedicle is non segment basal piece. On these antennae, hair may be present. At the base of each antenna, there is olfactory pin. Lateral part of head is known as “Gena”. Just below the frons, there is a chitinous plate known as “Clypeus”. Below clypeus, there is mouth part. Mouth parts are located on ventral side. Mouth part consists of labrum which forms the upper boundary of mouth and labium which forms the lower lip or lower boundary of mouth. On underneath the labrum, there is membranous structure called epipharynx which bears the organ of taste. On upper surface of labium, there is another membranous structure called hypopharynx which bears the opening of salivary duct. Between labrum and labium there is one pair of mandibles and one pair of maxillae. Mouth parts are collectively known as “Proboscis”.

 Family: Argasidae
 •The family includes fowl ticks and tampans.
 •The integument is leather like, frequently mammillated and without dorsal shield.
 •Capitulum and mouth parts are situated anteriorly and on ventral side, thus invisible from dorsal side.
 •In general eyes are absent, if present these are two pairs and situated on lateral sides of supracoxal folds.
 •Spiracles are one pair and situated posteriolaterally to the third coxae.
 •Sexual dimorphism is not evident.
 •The important genera are Argas, Otobius and Ornithodoros.

K ration: It is a lightweight packaged ration of emergency foods developed for the U.S. armed forces in World War II (Plate 371).

Kaiser rolls: A round crusty roll often used for sandwiches (Plate 372).

Kalamata /calamata: It is brine cured black olive grown in Greece.

Kamucha: Tea cider or fermented tea beverages is known as kamucha. The fermentation process take place by the presence of yeast and bacteria

Kanji: Clean, black coloured, crisp and sound product of characteristic flavor prepared by full fermentation (chiefly lactic acid fermentation) of black carrot with 2.5% salt, 1% powder mustard and some amount of water. It is fermented to make ready to serve drink which is known as Kanji. Each kilogram of the grated carrots yields just 7-8 litres of kanji.

Karl Fischer Titration: Karl Fischer Titration is used for estimation of moisture content of foods (low moisture products).

Kasseri cheese: The simple unpasteurized sheep’s milk cheese (sometimes mixed with goat’s milk) is called kasseri. It is popular in Greece. It is made in the shape of wheel, fairly firm and pale yellow (Plate 373). The word kasseri comes from the Turkish kasar which means that it has ability to hold its shape when heated.

U value: U value is a measure of the heat transmission through a building part (wall or window) or through a given thickness of a material (insulation). U values with lower numbers indicate better insulating properties (Plate 614).

UHT milk: This milk is homogenized, heated to atleast 135oC for about 2-3 second, cooled rapidly and then packed in sterilized containers aseptically. The milk in packs can be stored for several months without refrigeration. The nutrient loss is negligible. Used for making yogurt commercially as it does not need to be boiled again.

UHT pasteurization: The milk is heated to ~150oC for a fraction of seconds practically no holding period. It is also called ultra pasteurization.

UHT sterilization: It is method in which food is sterilized, cooled and then aseptically placed in previously sterilized containers which are subsequently sealed in an aseptic environment.

Organic products are grown under a system of agriculture without the use of chemical fertilizers and pesticides with an environmentally and socially responsible approach. This is a method of farming that works at grass root level preserving the reproductive and regenerative capacity of the soil, good plant nutrition, and sound soil management, produces nutritious food rich in vitality which has resistance to diseases. India is bestowed with lot of potential to produce all varieties of organic products due to its various agro climatic conditions. In several parts of the country, the inherited tradition of organic farming is an added advantage. This holds promise for the organic producers to tap the market which is growing steadily in the domestic and export sector.

As per the available statistics, India’s rank 8th in terms of World’s Organic Agricultural land and 1st in terms of total number of producers as per 2020 data (Source: FIBL & IFOAM Year Book, 2020). The APEDA, Ministry of Commerce & Industries, Government of India is implementing the National Programme for Organic Production (NPOP). The programme involves the accreditation of Certification Bodies, standards for organic production, promotion of organic farming and marketing etc. The NPOP standards for production and accreditation system have been recognized by European Commission and Switzerland for unprocessed plant products as equivalent to their country standards. Similarly, USDA has recognized NPOP conformity assessment procedures of accreditation as equivalent to that of NOP of US. With these recognitions, Indian organic products duly certified by the accredited certification bodies of India are accepted by the importing countries. APEDA is also in the process of bilateral equivalence with South Korea, Taiwan, Canada, Japan etc.

Pineapple, are well adapted crops for the farmers towards water economy due to its morphological, anatomical, and physiological characteristics. However, the plant contains rosette leaf structure can able to channels the light rainfalls and dew through stem and finally into the soil. It also have the ability to absorb water and nutrients through their waxy leaves and aerial roots and can capable to store water in specialized aquiferous leaf tissue; multicellular trichomes that cover the leaves and protect the plant from excessive transpiration by reflecting radiation; a thick cuticle covering the leaf epidermis that restricts water loss by evaporation, location of the stomata in furrows at the lower leaf surface where they also are covered by trichomes, limiting evapotranspiration; and its nocturnal carbon dioxide fixation via the crassulacean acid metabolism (CAM) carbon assimilation pathway, which results in stomatal opening primarily at night when evaporative demand is lowest (Reinhardt et al., 2018). The most common propagating material of pineapple is suckers or slips. Suckers are used to arise from the underground parts of the plant are used for propagation. Slips arise from the fruiting stem and the crown region on top of the fruit.

In this context, it is important to know the nutritional aspects of pineapples which are as attractive as their unique anatomy. “Pineapples contain high amounts of vitamin C and manganese,” said San Diego-based nutritionist Laura Flores. These tropical fruits are also important for the dietary fiber and bromelain (an enzyme). “As well as having high amounts of manganese, which is important for antioxidant defenses, pineapples also contain high amounts of thiamin, A, B vitamin that is involved in energy production,” Flores said. Pineapples are fat-free, cholesterol-free and low in sodium (Arpaia et al., 2004; De Costa, 2010). Side by side they contain sugars of about 14 grams per cup (www.livescience.com, 2019). In continuation with this, the chapter was focused on physiological disorder of pineapple caused by various reasons.

Basically, non-pathological conditions are the reasons of physiological disorders of any kind of crop plants; it includes poor light, adverse weather, water-logging, phytotoxic compounds or a lack of nutrients, which affects the normal functioning of the plant system. Sometimes physiological disorders may be initiated before harvest, continue their development after harvest, or appear after harvest. In was observed several times that physiological disorders are causative agent for the deterioration of horticultural commodities which leads to economic losses. An important commercialized fruit i.e., pineapple is also facing the same problem of different kind of physiological disorders; it includes multiple crowns, fruit and crown fasciation, collar of slips, dry fruit and bottle neck, sun-scald/ sunburn, blackheart etc.

10.1 Emerging Technologies and Innovations in Aquaculture

Aquaculture, the farming of aquatic organisms, is a rapidly growing sector within the global food production system. As the demand for seafood continues to rise, the industry is turning to innovative technologies and practices to ensure sustainable growth, improve productivity, and address environmental challenges. This article explores some of the emerging technologies and innovations that are shaping the future of aquaculture.

1. Recirculating Aquaculture Systems (RAS)

Definition and Concept: Recirculating Aquaculture Systems (RAS) are land based fish farming systems that reuse water by filtering and treating it before returning it to the tanks. This closed-loop system allows for precise control of water quality and environmental conditions.

Innovations and Benefits

• Water Conservation: RAS significantly reduces water usage compared to traditional open-net pen farming, making it suitable for areas with limited water resources.

• Environmental Impact: By treating and reusing water, RAS minimizes the release of pollutants and nutrients into natural water bodies, reducing the risk of environmental contamination.

• Environmental Impact: By treating and reusing water, RAS minimizes the release of pollutants and nutrients into natural water bodies, reducing the risk of environmental contamination.

BASIC PRINCIPLE FOR TREATMENT OF POISONING

Introduction

All the toxicants or poisons including various toxins causing various toxicoses or poisonings in animals comprise two major classes. Firstly, poisons having specific an tidotes and secondly, those having no specific antidotal therapy and require symptomatic and supportive therapy for the treatment of their poisonings. The adage "Treat the patient, not the poison" is the most basic principle of clini cal toxicology. Thus, in cases of both type of toxic conditions, an immediate attention is required for the maintenance of vital functions particularly cardiopulmonary function by providing supportive or therapeutic measures before institution of antidotal or symptomatic therapy. Vi tal signs and important reflexes should regularly be monitored to judge the response to therapy and progress of recovery. As per need of the situation, a flexile step wise practical approach may be opted by veterinarians for the diagnosis and treatment poisonings in animals.

? Lantana camara (lantana, wild sage, bunch berry) one of the ten most toxic weeds in the world. Brought to India in early part of the 19th century as an ornamental plant.

? Very hardy shrub grows very luxuriantly, capable of prospering in adverse soil.

? It has no. of adverse interactions with the biosphere.

? Causes hepatotoxicity, photosensitization in grazing livestock and growth inhibition of the neighboring vegetation due to allelopathic action which is rapidly changing the ecological balance.

? Reports of poisoning have been received form India, Australia, Cuba, Mexico, Kenya, Brazil and New Zealand.

? Very pungent plant. Cattle eat only in scarcity of other feed/fodder.

? Bos taurus more susceptible than Bos indicus.

Key Points

• Drowning is a common cause of accidental death in animals.

• Submersion in water or other fluids prevents atmospheric air from reaching the lungs.

• To diagnose drowning, trustworthy witnesses and a history of drowning at the accident location are required.

Introduction

Drowning or immersion is a type of asphyxial death caused by a body being submerged in water or another fluid medium and preventing atmospheric air from reaching the lungs. Drowning isn't always fatal. When a drowning causes death, it is known as a fatal drowning. Complete submersion is not required for lethal drowning; simply submerging the nose and mouth is sufficient. Drowning is a difficult process. It comprises liquid aspiration, struggle, involuntary movements, dyspnea, and cardiovascular responses. Drowning ultimately leads to hypoxia and death.

Key Points

• Traumatic injuries occur when an animal injures another or falls or strikes surrounding infrastructure (e.g., flooring, fence, gate).

• Common types of injuries include fractures, dislocations, bruises, and wounds, which might be penetrating or not.

Introduction

Physical injuries may occur throughout the export procedure. Slipping and being trampled or mounted during transportation or yarding are likely the most common causes of severe injuries. Poorly built or maintained facilities, such as yards, laneways, fences, gates, and flooring, can increase the risk of injury to animals while they are kept or handled. Poor stockmanship, such as rough handling and putting too much pressure on others, can also increase the chance of harm. Individual animals can be injured by improper restraint or handling, such as dragging an animal by its front or hind legs.

Key Points

• Cardiac crises can result in deadly outcomes. Prompt and precise diagnosis leads to early symptom alleviation and increased survival.

• Many illnesses qualify as cardiac crises, including thromboembolic disease, cardiac tamponade, arrhythmogenic disease, and congestive heart failure.

Introduction

A cardiovascular emergency is defined as any condition affecting the heart or vasculature that prevents appropriate blood flow, tissue perfusion, or oxygenation. Cardiac emergencies include a wide range of disorders such as arrhythmias, cardiac tamponade/pericardial effusions, and congestive heart failure (CHF).

Key Points

• In dairy cows, anestrus is a prevalent reproductive condition.

• In dairy farm animals, infertility, pyometra, and repeat breeder cows are the main reproductive syndromes.

Introduction

Farm animals have been raised and chosen specifically for the production of meat and dairy over the years. The livestock business suffers significant f inancial losses as a result of farm animal infertility. If the open span is extended by one estrous cycle as a result of infertility, this explains the 20–25 day loss of output in addition to the expense of treatment, missed work, etc. Farm animals can become infertile for a variety of reasons, including nutritional deficiencies, physiological disorders, and viral infections. These factors can act alone or in concert.

Key Points

• The main objective of managing open fractures is to neutralize further damage to the surrounding tissue while executing prompt, stable osteosynthesis and immobilization.

• Discomfort management should be considered because musculoskeletal diseases can cause severe discomfort.

Degenerative Myopathies

It is a degenerative disease of the musculature. Endocrine imbalances (hyperadrenocorticism, hypothyroidism, and hyperthyroidism), hypokalemia in cats, ischemic myonecrosis in recumbent cattle, post-exercise rhabdomyolysis, chemical agents, poisonous plants, and drug-induced degenerative conditions are some of the possible associations with it.

Key Points

• The most commonly cystitis and pyelonephritis occurs in farm and companion animals after parturition.

• Hematuria, pyuria, and stranguria are common presenting clinical symptoms;

• In acute situations, a prolonged course of treatment (two to four weeks) with the administration of the proper antimicrobials is usually successful.

Introduction

Both farm animals and companion animals frequently suffer from urinary infections, which are difficult to diagnose. In cows, urine testing is more helpful in excluding the illness if it shows any pus or germs (in females), and f inding the urethral calculus that is causing the urinary obstruction can be done by palpating the bladder using a rectum examination and palpating the urethral tube (in males). Because of the shorter urethra in females and the associated uterine infection that occurs during or after parturition, females are more likely to get urinary tract infections.

Key Points

• Animal euthanasia is the act of humanely killing animals, most commonly with injectable drugs.

• Euthanasia techniques vary by species, age, and the health status of the individual animal.

• Death must be confirmed before disposal of any animal remains.

• Animal remains must be disposed of in a legal manner that does not contaminate food sources or the environment.

Introduction

Euthanasia is the act of inducing humane death in an animal by a method that induces rapid loss of consciousness and death with a minimum of pain, discomfort, or distress. Euthanizing agents cause death by three basic mechanisms: (1) direct depression of neurons necessary for life function, (2) hypoxia, and (3) physical disruption of brain activity. Because loss of consciousness resulting from these mechanisms can occur at different rates, the suitability of a particular agent or method will depend on whether an animal experiences distress before loss of consciousness. Unconsciousness, defined as a loss of individual awareness, occurs when the brain’s ability to integrate information is blocked or disrupted. Ideally, euthanasia techniques cause rapid loss of consciousness, followed by cardiac or respiratory arrest and death.

Health benefits of fish consumption

Fisheries and aquaculture play an important role in providing food and nutritional security. Fish is a rich source of quality animal proteins, omega- 3: poly-unsaturated fatty acids (PUFAs), minerals, and vitamins. Fish is a superfood that is rich in all nutrients except carbohydrates, and its combination with rice or wheat makes it a complete food, forming a balanced diet with the right combination of macronutrients (quality proteins, fats, and carbohydrates) and micronutrients (minerals and vitamins), which are essential for good health.

Aquaculture is one of the fastest-growing food production sectors. India is bestowed with all types of aquatic habitats; freshwater, including the cold waters in the highlands, brackish waters, and the marine system. Being a tropical country, India is a biodiversity hotspot. It is rich in a wide variety of fish fauna, and wherever there is water, there are fish. Thus, fish is widely available in India, Thus, India is the third largest fish producing country and ranks second in aquaculture production. In comparison to other sources of animal proteins, fish is more chicken, mutton, beef, pork, etc., as affordable is available in wide varieties in the markets. Therefore, the common man can afford to consume fish regularly and provide his family with a balanced and healthy diet.

As a diet, fish is important for all age groups of humans. Fish oil, especially the omega-3 PUFAs, EPA, and DHA, keeps away several preventable human ailments (Table 1).

Canals and waterways are the most important source for irrigation and have been identified as one of the most important aquatic resources for fish culture. The natural canal systems in the Sundarbans cover an area of 907.33 ha. Fisheries activities were planned in order to provide a source of livelihood to the underprivileged people of the cyclone-prone area of the Sundarbans.

Challenges and opportunities

Fisheries in the Sundarbans are poorly documented and less attention is given to producing fish from canals. Information on the trophic status of the canal habitat, biotic community structure, and hydrological parameters is also lacking. However, the villagers reported that the fish enter these canals from the source waters naturally, and form natural populations. The villagers residing beside the canal catch fish, especially during monsoon, and the women of the adjoining households often collect small indigenous fish (SIFs) or prawns through small dragnets or scoop nets for household consumption. The shared information of the villagers was utilised to sensitise them and convince them to initiate fish culture in these canals.