eBook Chapters

1. Introduction

There are around six hundred major and minor crop species but only two percent are cultivated commercially meeting the food (calorie) requirements. The most commonly cultivated food plants are even smaller counting to merely 20 crops (Wilkes, 1984). The huge number of underutilized crops may serve as potential fruit crops to meet the nutritional requirements of poor farming households in lesser common farming and subsistence agriculture. The adoption of underutilized crops on a commercial scale may not be possible without crop improvement, standardized agronomic and fertilizer management technologies, pre- and post-harvesting techniques and marketing (Bag Mal et al., 1997).

Multipurpose trees and shrubs are defined as all woody perennials that are purposefully grown to provide more than one significant contribution to the production and/or service functions of a land-use system that they occupy. In agroforestry systems, different species of trees and shrubs can be planted with many types of crops in a variety of patterns. Thus, it is required to know about the methods of propagation and nursery raising, planting and other silvicultural management practices along with the diverse uses of multipurpose trees and shrubs of tropical and subtropical areas before their selection for forestry and agroforestry activities. It is important to select the most suitable tree species since it is not easy to replace them once they have been planted.

MANGIUM (Acacia mangium)

Acacia mangium is a single-stemmed evergreen tree that grows to 25-35 m in height and up to 60 cm in diameter. The bole is usually straight, often fluted near the base, free of branches for up to half its height. Mangium is native to Australia, Indonesia and Papua New Guinea. However, it tolerates varied site conditions and has adaptability to different planting objectives. Mangium shows most vigorous growth on well-drained, fertile soils in high rainfall areas in the humid tropics. It is valued for its rapid growth and has been planted throughout the humid tropics and is a major plantation species in the Asia-Pacific. Provenances from Papua New Guinea consistently show better growth in height and diameter.

Apple (Malus x domestica Borkh.) is a premium fruit of the world. It is a typical temperate fruit and main fruit crop of J&K State. The area under this fruit in J&K State is 1,62,971 hectares with an annual production of 17,26,834 metric tones. The acreage under this fruit crop in Jammu Division is 18,146 hectares and the annual production is 38,421 metric tones. In Jammu Division, apple is being grown in upper areas of Doda, Kishtwar, Ramban, Poonch, Udhampur, Reasi, Kathua and Rajouri districts.

Climate and soil

Apple is a typical temperate fruit crop requiring 1000-1600 hours of rest period below 7^oC. The average temperature during growing season should be 21⁰C to 24⁰C. Sunshine is essential for colour development of the fruit. In general, a cool climate with low winter temperature and little rainfall in summer is most suitable for apple cultivation.

Apple is grown on a wide range of soils. However, a well drained slightly acidic (about 6.5 -6.7 pH) loam soil with good depth (more than 45 cm) is ideal for apple cultivation. Apple can’t tolerate wet field, and water logging may cause collar rot and death of trees.

Ber (Zizyphus mauritiana Lamk.) is one of the ancient and common fruits, indigenous to India. It is growing in wild, semi wild and cultivated forms all over the country in sub-tropical and tropical areas. Area under ber in J&K is 5,376 hectare with annual production of 10,779 metric tonnes.

Climate and Soil

Ber plant is hardy and can grow successfully even under poor soil and unfavourable climatic conditions, where most other fruit trees fail to grow. It prefers a hot and dry climate for its successful cultivation, but needs adequate water during its first fruiting season. It can be grown up to an elevation of 1000 meters above sea level. Excessive atmospheric humidity seems to be a limiting factor in its successful fruiting.

The ber tree develops a deep tap root system with in a short period of its growth and as such adopts itself to wide variety of soils. However, deep sandyloam soils appear to be better for its plantations. It is well known in its ability to thrive under adverse conditions of salinity, drought and water logging.

Citrus (Citrus spp.) is one of the most important fruits grown in sub-tropical and foot hill regions of Jammu province (Kathua, Hiranagar, Samba, Jammu, Akhnoor, parts of Basohli, Nowshera, Ramnagar, Ramban and Udhampur) upto an elevation of 800 m amsl. The most important commercial citrus fruits in Jammu region are kinnow mandarin, sweet orange, grapefruit, lemon and lime. The area under citrus fruits in Jammu division of state is 14,542 ha with an annual production of 34,191 metric tones.

Climate and soil

Citrus plants being sub-tropical cannot with stand extended cold period. Temperature below 3^oC and above 40^oC are injurious to the citrus. Hot winds and excessive heat during flowering and fruit set cause fruit drop and sun burning. These are the light loving crops.

Citrus fruits thrive well in deep, loose, well aerated soils with the pH of 5.5 to 6.2 devoid of any hard pan layers of calcium carbonate in the rooting zone up to 150 cm. High water table and poor drainage system are quite unsuitable for citrus fruits which cause drying of roots and nutritional imbalance, respectively.

Guava (Psidium guajava), the apple of tropics, is one of the most common fruits in India. It is the fourth most important fruit in area and production after mango, banana and citrus. Being hardy, it gives assured crop even with little care, and is good for rainfed areas also. This fruit is popularly being grown in sub-tropical regions of Jammu on 2480 ha with annual production of 9153 MT.

Climate and Soil

Guava is successfully grown under tropical and sub-tropical climate. In areas with distinct winter season, the yield tends to increase and quality improves. It can be grown upto an altitude of 1515 m above mean sea level. Older plants can tolerate drought. High temperature at the time of fruit development can cause fruit drop.

Guava trees are very hardy and can thrive on all types of soils, but are sensitive to water logging. The best soils are deep, loamy and well drained.

The litchi (Litchi chinensis) is a delicious juicy fruit of excellent quality. It is liked very much as table fruit. Presently, litchi occupies 940 ha area in Jammu region with an annual production of 2315 MT.

Climate and Soil

Litchi being a sub-tropical fruit thrives best under warm humid sub-tropical climate. However, frost in winter and hot winds in summer limits its growth and production. Hot winds results in cracking of fruits before reaching maturity while excessive rainfall at the time of flowering interfere with pollination. The prolonged dry spell after fruit set reduces the crop considerably.

Litchi flourishes well on deep, well drained loamy soil, rich in organic matter and free from hard substrata.

Mango (Mangifera indica L.) is one of the choicest and most ancient fruits known to mankind. It occupies a pre-eminent place amongst the subtropical fruits grown in Jammu region and occupies an area of 12,739 ha with annual yield of 24,150 metric tons. It is extensively grown in Jammu district and also in the sub-tropical areas of Udhampur, Reasi, Kathua and Rajouri districts.

Climate and Soil

Mango thrives well up to 600 m above mean sea level provided locality is frost free and there is no high humidity or rains during flowering. The favourable temperature is 24⁰ C to 27⁰ C, however, it can tolerate temperature as high as 48⁰ C provided that trees are getting regular irrigation.

Mango has been found to grow on a wide range of soils. However, deep and well-drained sandy loam soils are most suitable for its cultivation. Heavy black cotton, saline and alkaline soils should be avoided. The ideal range of soil pH for mango cultivation is 5.5 to 7.5.

Peach (Prunus persica (L.) Batsch.) is a highly popular fruit crop grown in temperate regions of the world. In India, peach cultivation is confined to mid hill zone of Himalayas extending from J&K State to Khasi Hill at an altitude of 1500 to 2000 meters above mean sea level. Low chilling cultivars are also grown in sub mountainous zones of Punjab, Haryana, Delhi and Western U.P. and also gaining popularity in sub-tropical areas of Jammu. Peach crop in J&K state covers an area of 2,615 hectares with 1,699 hectares falling in Jammu Division. Total production of peach in J&K state is 6,221 metric tonnes, out of which 2,612 metric tonnes are being contributed by Jammu Division. Main peach growing areas in Jammu region are hilly areas of Poonch, Doda, Kishtwar, Ramban Udhampur, Reasi, Rajouri and Kathua districts. Recently, the new orchards of low chill peaches are also being planted in Jammu, Samba and Kathua districts.

Climate and Soil

Peach does well in wet and cool climate with cold winter and dry summer. It likes mild climate. The limiting factors in peach cultivation are the minimum winter temperature, chilling hours, spring frost, hail storms, high humidity and desiccating winds during summer. In sub-tropical areas of North, the low chilling cultivars require 50-300 hours of chilling during the winter for proper flowering and fruiting.

The peach thrives well on light textured soil. Deep, fertile, loam or sandy loam with good drainage is considered well for successful peach cultivation. Poorly drained soil should be rejected for peach cultivation.

Pear (Pyrus spp.) is a superior temperate fruit with good taste and flavour. Because of its tolerance to wide range of soil and climatic conditions, it is grown both in sub-tropical and temperate conditions. In J&K, 14,532 hectare area is under cultivation, with an annual production of 88,329 metric tones. In Jammu Division, pear is being grown on 7,600 hectare having 30,257 metric tonnes production annually. The pear is grown in Doda, Poonch, Rajouri, Udhampur and Kathua districts of Jammu Division.

Climate and Soil

Pear is adapted to wide range of climatic conditions. Most of the commercial cultivars require 900-1000 hours of chilling below 7^oC to break the dormancy. Pear is a temperate fruit crop; however, it can be grown successfully in subtropical regions of Jammu. There are several low chilling cultivars, which are well adapted for cultivation under sub-tropical conditions. Pears are grown successfully over a wide range of soils provided they have sufficient moisture. Compared to apples, they are less tolerant to drought and rather more tolerant to impeded drainage. Deep, well drained and fertile soil give high yield.

Pecan (Carya illinoensis) is a highly nutritive and energy giving nut fruit. It has nutty flavor and superb tree characteristics. Area under pecannut in J&K is 414 hectares with annual production of 86 metric tonnes.

Climate and Soil

Pecan need warm temperate climate and extremes of both temperate and sub-tropical climates are undesirable. It needs adequate chilling hours ranging from 400-600 hours below 7.2^oC for different cultivars. Mean monthly temperature ranging from 24.0 to 29.5⁰C, with little diurnal variation during growing period is best for growth and fruiting. High humidity prevents pollination, increases the incidence of diseases and premature splitting of nuts.

Although pecan nuts are grown in varied soil conditions, but sandy loam soils (pH 5.0-8.0) which are deep, fertile and are rich in organic matter are best for its growth and fruiting. Symbiotic mycorrhiza and fibrous roots permeate the top 1 meter of soil.

Phalsa (Grewia subinaequalis) is one of the hardiest, drought resistant and quick growing fruit crop. Different parts of the plant are used for curing variety of ailments. The ripe phalsa fruits are consumed fresh, in desserts or processed into refreshing fruit and soft drinks enjoyed during hot summers.

Climate and Soil

Phalsa prefers dry conditions and can therefore, withstand drought. It flourishes well under variable climatic conditions, and requires protection from the freezing cold temperature. Also, adequate sunlight and warm or hot temperatures are required for fruit ripening, development of appropriate fruit colour and good eating quality. Kandi areas are, therefore, quite suitable for its cultivation. It can also be grown under irrigated conditions. Phalsa plant grows vigorously under wide range of soils including fine sand, clay or even limestone. However, best results are obtained in well-drained, loamy soils.

Plum (Prunus saliciana Lindl.) is a temperate fruit and grows on commercial scale in J&K, HP and Uttaranchal. In J&K, plum cultivation is spread over 4,038 hectares with total yield of 10,112 metric tones. In Jammu Division, plum is being grown in temperate areas of Poonch, Rajouri, Doda and Kathua districts covering an area of 2,665 hectares with an annual production of 14,240 metric tonnes.

Climate and Soil

Plums because of their wide variability between different species, exhibit considerate varietal differences in their climatic requirements. The Japanese plums do very well in the low and mid hill regions ranging between 650 to 1650 meters above mean sea level. Some varieties can be grown successfully in the warmer regions of plains.

For good growth and longer life of plants, well drained medium to deep soils are most suitable. Plums propagated on peach root stocks grow better on sandy loam soil, while for heavier soils apricot rootstock or Kabul green gage root stocks of plum can be used.

Walnuts (Juglans regia L.) are found in the northern parts of the country in temperate belts. They are grown in the length and breadth of the valley in Jammu and Kashmir and hilly areas of Jammu. The walnut is being cultivated in the state on 89,339 ha with an annual production of about 2,66,280 metric tonnes. In Jammu Division, walnut plantation is on 38,318 ha with an annual production of 92,227 metric tonnes. The walnut is very rich in proteins, fats and minerals and is a concerned source of energy.

Climate and Soil

Walnuts are found growing in all parts of the Himalayan region between the elevation of 1200 to 2100 m above mean sea level. Walnuts are sensitive to both low and high temperature. Climate should be free from frost in spring and from extreme heat in summer. They grow well in areas where rainfall is about 75 cm or more and is well distributed. Temperature of 29-32^oC near harvest time, results in well-filled kernels.

Walnuts do best in soils where roots can penetrate deep evenly. In addition to good top soil, sub-soil must be free of rock, impervious clay or layers of gravel. Most suitable soil is well drained silt loam with abundant organic matter. Alkaline soil and sandy soils should be avoided for walnut growing. A soil with 2-3 metres depth and pH range of 6-7 gives best results.

In practice, flowers are collected from wild plantations. Recently kewda has emerged as one of the important commercial commodities. A systematic cultivation practice is essential to develop a successful plantation and to increase the productivity.

Planting

Kewda suckers or crowns are generally planted during June-July. i.e. in the beginning of the monsoon, when the soil is not too wet. Planting in wet soil delayd the root formation and reduces the success level. Just after the first shower of rain or during the summer shower close to monsoon, kewda is planted.

In a full grown plant, stilt roots originate at a few feet above the ground to render a support for the huge canopy of the plant. Each part of the stem is capable enough for development of root initials, but it is retarded once the stem became old and leafless. The root initials develop at the terminal portion, where more leaves cluster, but they do not grow. Hence, the stem cuttings collected from the flowering or non-flowering shoots bear the rudimentary roots and when planted, they establish quickly.

In practice, the cuttings are collected and scattered on the ground for two to three days. Before planting, a few leaves closed to the base of the cuttings are removed to expose the root development sites. The cuttings are planted in trenches of 45 cm depth horizontally or at an angle of 30^o. The new leaves appear on the growing points one month after planting.

Kewda plants are usually planted on the field bunds for fencing and subsequently used for collection of flowers. A 30 years old plant can produce a crown of 6-7 m, for which it is difficult to develop a commercial plantation in a close spacing. It has been observed in the natural plantations that the plants may be spaced at 8-10 m distance to achieve a full length of plantation life. It is, therefore, suggested by the authors to follow a paired row method of planting maintaining a spacing of 4 m between plants and rows of a paired row and 6 m between two paired rows; which provides all facilities for plant growth upto 10-12 years and then pruning of a few branches are required. Hence, a plant population of 500 plants in the field and 150-200 plants on field bunds are maintained in a land of one hectare.

23.1 Introduction

Cultural methods have been in practice for pest management in traditional systems of agriculture since time immemorial. The worldwide awareness of safe environment provided the impetus to foster non-chemical pest management strategies. Of these strategies, cultural control constitutes the most farmer-oriented approach, where the weak points in the biology and behaviour of insects are exploited and pressure is exerted on the population by manipulating the environment.  In addition to the cultural practices, the other widely used approach is the use of physical control methods which hold promise and provide an attractive alternative to current practices, particularly when integrated into a general control scheme incorporating cultural methods, plant breeding, biological or behavioural methods and pesticides. In physical control methods, the physical environment of the pest is modified in such a way that the insects no longer pose a threat to the agricultural crop.

Cultural characteristics of bacteria deal with the appearance of the growth in various liquid and on solid media. The growth characteristics of most of the bacteria are typical of the group or species and help in their identification. The terminology employed to describe various features of bacterial growth are mentioned below.

Soil and Climate

Commercial pomegranate orchards are confined mainly to Mediterranean, and semi-arid regions of the world. It is hardy fruit, which can be grown successfully, even in low fertile soils. Although pomegranate of high quality can be grown only where there is a cool winter and a hot dry summer, the trees grow under wide range of climatic conditions. It can be grown from the plains to an elevation of about 1829 m. The tree can withstand frost but gets injured by temperatures below 11.1^ºC.

In areas of low winter temperatures the tree is deciduous but under tropical and sub-tropical conditions, it is evergreen or partially deciduous. The tree cannot produce sweet fruits, unless the temperatures are high for sufficiently long period. The quality of fruit is adversely affected in humid climate.

INTRODUCTION

The Cultural practices which includes manipulation /or adjustment of crop production techniques have been as old as possibly agriculture itself. In early stages of agriculture development, the growers through their experiences and observations had known that repeated cultivation of a particular crop species or variety on a piece of land often resulted in crop sickness. By proper crop rotations they had been avoiding such sickness. As a matter of fact, in the present day agriculture, cultural practices are being considered as essential backup methods for plant disease management. Cultural practices often offer the opportunity to alter the environment, the condition of the host, and/or the behavior of the causal agent, to achieve economic management of disease. Most cultural practices used to control plant disease are preventive in nature. Integration of cultural practices, host resistance and pesticides or biocontrol agents may be necessary to provide options for controlling economically important plant diseases

Introduction

Cultural management is defined as manipulation /or adjustment of crop production techniques for management of plant diseases. Cultural practices have been as old as possibly agriculture itself. In early stage of agriculture development, the growers through their experiences and observations had known that repeated cultivation of a particular crop species or variety on a piece of land often resulted in crop sickness. As a matter of fact, in the present day agriculture, cultural practices are being considered as essential backup methods for management of plant diseases. Adequate adjustment in crop production techniques can modify the environment in such a manner that it becomes unfavorable for the pathogen and pathogenesis. Based on this the disease control affected by cultural practices are preventive, these practices aim at reducing the activity and density of inoculum. It offer the opportunity to alter the environment, the condition of the host, behavior of the causal agent to achieve economic management of disease.

Introduction

Cultural management is defined as manipulation /or adjustment of crop production techniques for management of plant diseases. Cultural practices have been as old as possibly agriculture itself. In early stage of agriculture development, the growers through their experiences and observations had known that repeated cultivation of a particular crop species or variety on a piece of land often resulted in crop sickness. As a matter of fact, in the present day agriculture, cultural practices are being considered as essential backup methods for management of plant diseases. Adequate adjustment in crop production techniques can modify the environment in such a manner that it becomes unfavorable for the pathogen and pathogenesis. Based on this the disease control affected by cultural practices are preventive, these practices aim at reducing the activity and density of inoculum. It offer the opportunity to alter the environment, the condition of the host, behavior of the causal agent to achieve economic management of disease.

Introduction.
The Cultural practices which includes manipulation /or adjustment of crop production techniques have been as old as possibly agriculture itself. In early stages of agriculture development, the growers through their experiences and observations had known that repeated cultivation of a particular crop species or variety on a piece of land often resulted in crop diseases. By proper crop rotations they had been avoiding such diseases. As a matter of fact, in the present day agriculture, cultural practices are being considered as essential backup methods for plant disease management. Cultural practices often offer the opportunity to alter the environment, the condition of the host, and/or the behavior of the causal agent, to achieve economic management of disease. Most cultural practices used to control plant disease are preventive in nature. Integration of cultural practices, host resistance and pesticides or biocontrol agents may be necessary to provide options for controlling economically important plant diseases.

Cultural methods of plant disease management refer to the application of agronomic practices that help reduce the incidence and severity of diseases. These methods emphasize creating conditions that are unfavourable for pathogen development and spread while promoting the health and vigour of the crop. They are sustainable and environmentally friendly, as they often involve practices that can be integrated into routine crop management.
In this chapter, we will explore various cultural practices, including crop rotation, sanitation, planting time and spacing, irrigation management, and more. These techniques form the foundation of integrated disease management (IDM) and can be used in combination with other control measures for effective disease control.
Importance of Cultural Methods in Disease Management
Cultural practices serve as preventive measures to minimize disease outbreaks by influencing the interaction between the host, pathogen, and environment. By modifying farming practices, it is possible to:
a) Reduce Pathogen Inoculum: Prevent the initial development of pathogens in the field.
b) Limit Pathogen Spread: Break the life cycle of pathogens and reduce the spread to healthy plants.

1. INTRODUCTION

Medicinal plants constitute considerably a large component of natural vegetation. Several of these species are in great demand for domestic consumption as well as for commercial use by the herbal industry. About 800 species are estimated to be in trade with a turnover of Rs.4000 crores per year. This high demand by the herbal industry has put enormous pressure on the wild populations leading to destructive collection of the produce as maximum species of medicinal plants are wild.

A medicinal plant is as any plant which contains substances that can be used for therapeutic purposes or which are precursor for chemo- pharmaceutical synthesis. The medicinal plants are useful as a drug or therapeutic agent, having different medicinal values in form of active ingredient. Medicinal plants may therefore be defined as a group of plants that possess some special properties or virtues which make them important as drugs, therapeutic agents and for medicinal purposes.

Medicines from India are used by about 60 per cent of the world’s population. These are not only used for primary health care not just in rural areas in developing countries, but also in developed countries as well where modern medicines are predominantly used. While the traditional medicines are derived from medicinal plants, the herbal drugs are prepared from medicinal plants only. Use of plants as a source of medicine has been an ancient practice and is an important component of the health care system in India. There are about 45,000 medicinal plant species in India, with concentrated spots in the region of Eastern Himalayas, Western Himalayas, Western Ghats and Andaman & Nicobar Island. India is the largest producer of medicinal herbs and is called the botanical garden of the world. In rural India, 70 per cent of the population depends on the traditional type of medicine, the Ayurveda. Ayurveda and Kabiraji (herbal medicine) are two important forms of alternative medicine that is widely available in India. It is estimated that about 80,000 species of plants are utilized in some form or other by the different systems of Indian medicine. Many herbs and spices are used in Indian cooking, such as onion, garlic, ginger, turmeric, clove, cardamom, cinnamom, cumin, coriander, fenugreek, fennel, ajowan (ajwain), anise, amchur, bay leaf, hing (asafoetida) etc. Ayurvedic medicine uses all of these either in diet or as a medicine.

Pineapple is one of the most economically important fruits in tropical and subtropical regions and gaining popularity to the consumers all over the world. The pineapple plant has many specific morphological, anatomical and physiological characteristics that determine crucial aspects of pineapple crop management, such as water use efficiency, nutrient management, weed management, flower induction etc.

Water Requirements and Irrigation

Determination of water requirements and plant moisture status: Pineapple plant responds well to as much as 5 cm of water per month from rain or irrigation though it is a xerophyte type and is capable of good production under relatively meager water supply. Maintenance of available soil moisture in the root zone areas requires less water for pineapple than for other crops due to lower transpiration rates. Plants achieve this type of adaptability through a process called assuberization, where suberin act as a physical barrier, preventing water loss from the tissues it surrounds.Stomata remains closed as an effect of drought during the entire day. But, whenever the plants are grown with sufficient moisture in well-aerated soils produced well-distributed roots with numerous white root tips. It helps the water use efficiency and carbon f ixation that pineapple shows to drip irrigation. The pineapple leaf is a major water storage organ for the pineapple plant (Malézieux et al., 1993). When the leaves are full of water the cells present in upper mesophyll layer can occupy almost half of its volume. Invery little water-supply, the water in the mesophyll layer is absorbed by the plant. Generally, representative ‘D’-leaves are taken in a group of six to ten leaves and the total thickness is measured and compared with the thickness of fully turgid leaves.

Extension education serves as a catalyst for altering the behavioral complexities within rural communities. This behavioral shift is intricately intertwined with cultural determinants, thereby necessitating extension workers to possess a profound understanding of rural cultural dynamics. Culture, delineated as learned behavior organized into patterns and disseminated among society members, constitutes a foundational concept in sociology, elucidating the regularities observed in human actions. Unlike its colloquial interpretation, which often limits culture to refined expressions such as art and music, sociological discourse expands the scope to encompass the entirety of learned behaviors, attitudes, beliefs, and social norms characteristic of a specific group.

What is subculture?

In cell culture, a process called subculturing, or passaging, keeps cells alive and growing. It involves taking cells from an existing container, removing the used medium, and placing them in a new container with fresh nutrients. This allows the cells to continue multiplying and creates more cells for research.

Adherent subculture protocol Cells nearing 80% coverage need splitting!

1. Warm things up: Get your media, detachment solution, and incubator ready at 98.6°F (37°C).

2. Safety first! Disinfect your work area and supplies inside a biosafety cabinet.

3. Gently rinse: Remove the old media and wash the cells with a salt solution at room temperature. Be careful not to dislodge the cells.

4. Detach the cells: Add a pre-warmed enzyme solution to detach the cells from the flask surface. Keep an eye on them to make sure it works (usually 2 minutes). Note: Not all cells need this enzyme, and it can be harmful to some.

5. Stop the enzyme: Once all the cells are detached, add a culture medium with serum to neutralize the enzyme.

6. Collect the cells: Transfer the cell suspension to a tube, rinse the flask with fresh medium to get all the cells, and add it to the same tube.

7. Spin it down: Centrifuge the cell suspension for 5 minutes at room temperature (1000 rpm).

8. Get ready for counting: Remove the liquid, loosen the cell clump, and resuspend the cells in a fresh medium at a good volume for counting.

9. Count your cells: Use a hemocytometer to count the cells and determine their health.

14.1 Culture from Urine

Infection of urinary tract due to microorganisms affect patient of all age group and both sex., and they vary in severity from an unsuspected infection to a condition of severe systemic disease. Urine is a ordinarily excellent culture medium for the multiplication of common pathogen of urinary tract. Most bacteria found in the urine are due to contamination of skin flora during passage and they tend to increase greatly, exceeding 10⁶/ml. When the bacterial count exceeds 10⁵ per ml of urine it indicates significant bacteriuria and is indicative of a urinary tract infection. Infection of the urinary tract may be limited to a single tissue/ organ or they may spread upward and involve the entire urinary system. Escherichia coli is the most common urinary tract pathogen. Pseudomonas aeroginosa causes another important urinary tract infection, which is characterized by blue green pus. Cystitis (inflammation of urinary bladder), Pyelonephritis (inflammation of the kidney) are permanent diseases of urinary tract.

Urinary tract infections are generally characterized by bacteriuria, bacteria in the urine. Quantitative microbiological assay of urine can identify the probable pathogen. For this purpose, calibrated loop- direct streak method and pour plate method are routinely used. Now-a-days commercially prepared special plastic device called the urine dip slide and diagnostic urine–culture called Bacturcult is being used for isolation and identification of microorganisms from genitourinary tract infections. The Bacturcult is a sterile disposable plastic tube coated with a special medium on its inner side for detection and identification of bacteriuria.

Culture methods employed depend on the purpose for which they are intended. In the laboratory the indications for culture are mainly to

1.Isolate bacteria in pure culture.

2.Demonstrate their properties

3.Obtain sufficient growth for preparation of antigens and for other tests.

4.Type isolates by methods such as bacteriophages and bacteriocin susceptibility.

5.Determine sensitivity to antibiotics

6.Estimate viable counts

7.Maintain stock solution

1. Catla prefers zooplankton.

2. Silver carp prefers phytoplankton.

3. The blooming of diatoms causes golden brown colour in the aquaculture system.

4. Light or bright green colour of pond is due Chlorella (Green algae).

5. Dark green or blackish green colour is due to BG algae (Oscillatoria, Phormidium, Microcoleus).

6. Dark brown colour or sauce like colour is due to eutrophication.

7. Yellowish colour of pond water is due to growth in Chrysophyta.

8. Foggy white colour of pond is denoted by the mixture of zooplankton, clay particles and detrirus.

9. Japan is the biggest producer of marine finfish fry of 200 million fry per year (Pagrus major, Paralichthys olivaceus makes up 70% of total production).

10. Europe is a second biggest producer of marine fish fry for about 100 million fry per year.

Bone marrow contains variety of tissues including hematopoietic lineage cells and mesenchymal stem cells (MSCs). The hematopoietic cells are the major source of the blood cells in the adult body which are regulated within the microenvironment of the stromal cells of the bone marrow. MSCs are multipotent cells and can be differentiate into various cell lineage depend upon their environment and culture condition in which they are kept. Rat bone marrow derived stem cells (rBM-MSCs) have long term self renewing and capabilities of pluripotency including osteoblast, adipocytes, chondrocytes, tenocytes, muscle cells and neurogenic cells, which make them ideal source of stem cells for regeneration of injured tissue.

Bone marrow derived mesenchymal stem cells have been isolated from different species. Each species is having different culturing and growing capacity. For example human bone marrow derived stem cells are easy to harvest and maintain in culture condition whereas rat MSCs it is more difficult compared to other species. Technical difficulties in isolation limited the number of animal experiment and its required animal transplantation model for pre clinical studies. Density gradient centrifugation, plastic adherence and immunomagnetic selection like various methods are available for isolation of MSCs from bone marrow. No appropriate methods are available for selection of suitable cells. Each method has their own pros and cons. Therefore, in this chapter, combined density gradient centrifugation and plastic adherence as easy and reliable method for isolation, culture and characterization of rat bone marrow derived mesenchymal stem cell is described.

7.1. Culturing of Nematodes

The need to use host plant tissue makes the culture of phytonematodes more difficult and expensive than the culture of fungi and bacteria. Relatively few species of phytonematodes have been cultured, compared to fungi and bacteria. Populations derived out of extraction procedures are often not sterile, an additional hindrance to many types of biological studies.

Axenic culture of free living, insect/animal parasitic nematodes in chemically defined or nondefined media has been achieved with some success, but development of similar culture system for plant parasitic nematodes has been less compared to former ones. However, these techniques form useful research tools in Nematology, which help procure clean nematodes, free of contamination for wide range of studies. Dual culture of nematode and plant tissue is aimed to establish plant parasitic nematodes in sterile cultures. Since phytophagous nematodes are obligate parasites they need a plant tissue as a food source. The term ’Gnotobiology’ is normally used to culture/study a single species of a nematode in the absence of other nematodes /organisms or in the presence of other known species. These techniques are generally considered as tedious and time consuming, require expertise and specialized equipments, nematodes are rarely found associated with only one or few other organisms and there is a need to get non-sterile soil and/or plant tissues, which does not go with gnotobiotic techniques.

4. 1. Origin and distribution

• Native to Egypt, Syria, Middle East and stretching east to India.

• In India, cumin is mainly cultivated in Western Indian states like Rajasthan and Gujarat.

4.2. Economic importance

• Important spice used in Indian kitchens for flavoring various food preparations.

• The flavor is due to chief constituent cuminol (Cuminaldehyde)

• Volatile oil is up to 2.5-3.5 %.

• Used in various ayurvedic medicines especially for the conditions like obesity, stomach pain and dyspesia.

• Many Latino, Vietnamese, United States and Thai foods recipes have cumin and many chilli recipe includes cumin.

• In India, cumin is a traditional ingredient not only curry, but also kormas, masalas, soups and other recipes.

• Used in some cheeses like Leyden cheese as well as some French breads.

1.0 INTRODUCTION

The cumin (Cuminum cyminum Linn.) belonging to family Apiaceace is an important seed spice believed to be indigenous to northern Egypt, East Mediterranean region, Iran, Turkey  and India (Amin, 2003; Raghavan, 2007). It is popularly known as safed jeera and is one of the most common spices of daily use in the Indian sub-continent. Cumin is well known to ancient civilization. It is one of the oldest spices known since biblical times, valued mostly for their digestive properties and for flaovouring bread and other dishes during the periods of ceremonial fasting. As early as 5000 BC, Egyptians preserved the bodies of their kings by mummifying them with cumin, anise and marjoram.

English name : Cumin
Botanical name : Cuminum cyminum L.
Family : Apiaceae
Chromosome number : 2n = 14
Centre of origin : Egypt, Syria and Turkey
Cumin (Cuminum cyminum L.), also known as Safaid jeera, is one of the oldest spices in history. It is derived from the mature fruit of a slender herb. Cumin seeds range from yellowish to greyish brown and emit an aromatic scent due to cuminol and aromatic alcohol, contributing to their distinct spicy and slightly bitter taste. These seeds are widely used as a condiment. The essential oil of cumin seeds contains various compounds, including a-pinene (14.3-19.7%), p-cymene (2.7-6.0%), terpinene (11.5-16.2%), cuminaldehyde (20-22.4%), p-menth-1,3-dien-7-al (11.1-13.5%), p-menth-1,4-dien-7-al (23.6-24.3%), cuminyl alcohol (2.7-72.2%), sabinene (3.38%), benzyl cinnamate (4.47%), and farnesol (2.98%). Cumin is an essential component of many spice blends and curry powders and is used to flavour soups, pastries, stews, sausages, pickles, cheese, and bakery products like bread and cakes. Cumin oil finds its place in soap making, perfumery, and flavouring alcoholic beverages such as liquors and cordials, with absolute oil being preferred for flavouring purposes. Cumin aldehyde is also utilized in perfumery.

Curing- Curing is a process in which produce is subjected to undergow through a special treatment to get the quality product.

Genus   : Ribes 
Species : nigrum L. americana Mill.
                bracteosum Dougl. niveum 
                Lindl. divaricatum Dougl.
Family : Saxifragaceae / Grossulariaceae
Chromosome No. : 2n = 16

The genus Ribes belongs to the family Saxifragaceae mainly comprises of woody shrubs distinctly marked with spines and prickles and those which have attractive handsome foliage, flowers and fruits.  The genus name Ribes is likely to be derived from ribas, the Arabic name for Rheum Ribes. Some are of the view that it is the latinized form of riebs,  an old name of currants in Germany.

ABSTRACT

The concept and nature of intellectual property have undergone a paradigm shift during the last few decades. The importance of effective IPR governance has attracted the attention of its stakeholders. Hence, this paper focuses on the role of IPR governance and management practices for improving the quality of relationships among stakeholders besides documenting current and future trends of IPR governance and management.

INTELLECTUAL PROPERTY RIGHTS – AN INTRODUCTION

Intellectual property rights refer to the privileges granted by the government to any individual or organization for creative prowess. In recent years, such rights have gained immense importance. The laws in existence for protection and enforcement of these rights are meant to foster innovation and for regulating the use of inventions.

Introduction 
The discovery and introduction of antimicrobial agents to clinical medicine was one of the greatest medical triumphs of the 20th century that revolutionized the treatment of bacterial infections. However, the gradual emergence of populations of antimicrobial resistant pathogenic bacteria, resulting from use, misuse and abuse of antimicrobials has today become a major global health concern. Antibacterial drugs have revolutionized our ability to control bacterial disease, and their clinical availability has led to dramatic decreases in morbidity and mortality. (Walsh and Wright, 2005). As such, these therapeutics underpin modern medicine. Despite the integral role of antibiotics in sustaining our modern lifestyle, they are undervalued in both cost and significance by society. Over the past century, their use has provided strong selective pressure on microorganisms, leading to preferential survival and spread of those harbouring antibiotic resistance mechanisms. Multidrug resistance is now commonplace amongst bacterial pathogens with antibiotic resistance now affecting all antibiotic classes. (Coates et al, 2011). This is particularly worrisome in the case of Gram-negative bacteria (e.g. Pseudomonas aeruginosa and Acinetobacter baumannii) for which treatment options are already limited. The ‘broken’ economics of antibacterial research and development is often quoted as the main reason for the lack of new therapies but the truth is that it is hard to discover new antibacterial drugs, and the science is not sufficiently advanced to allow discovery of efficient and effective drugs. This has led to fears of a ‘post-antibiotic era’ as it has been estimated that 5–20 novel antibacterial drugs need to enter clinical development in order to effectively contend with the current resistance problem. However, given the attrition rate within the existing drug discovery model, at least 200 discovery programmes would be needed in order to achieve this outcome. Hence, new approaches to antibiotic discovery are needed. The antibiotic pipeline The antibiotic pipeline is not what it once was.4 Pharmaceutical companies were once the main provider of novel antibiotic molecules but they largely withdrew in the late 1990s because of the lack of success and low financial returns in bringing new antibacterial drugs to the market.5 The environment of discovering and developing new antibiotics was quite different during the so-called ‘golden era’ of drug discovery. Antibiotics worked remarkably well because resistance was low and physicians had access to a variety of efficacious antibiotics. Antibiotic research and development programmes were inclined to focus on improved pharmacology to achieve less frequent dosing e.g. once a day, rather than innovative new antibiotics. Natural product screening strategies tended to result in rediscovery of compounds rather than finding new ones. There was also no need to consider those natural products with undesirable properties such as toxicity. Today, only a few large companies such as GlaxoSmithKline, Novartis, Merck and Roche are still actively engaged in antibiotic R&D, with many of the original antibiotic provide.The natural world remains the largest source of novel drug scaffolds making this a viable option in the search for new antibiotic compounds. Advances in bacterial culture techniques, molecular biology and metagenomics will make natural product drug discovery easier and more costeffective, obviating these limiting factors. Screening procedures must include whole-bacterial cell assays, addressing the issue of bacterial permeability and efflux early in the discovery process (Shore and Coukell, 2016). Additionally, the generation of training schemes by, and with, pharmaceutical companies that cover all aspects of the pipeline and include natural product drug discovery, are essential and will ensure that expertise is passed on to future researchers. Investment should also be made into the study of previously characterized lead compounds that did not reach the clinic, so called old leads. The reasons that led to these compounds being dropped from further development vary, ranging from financial issues to trial design, dosing problems and toxicity. It may be that there is now sufficiently improved technology and expertise to develop these as safe and efficacious antibacterials. The revival of interest in old leads could also provide an additional source of novel antimicrobials. A freely accessible database of antibiotics that were never developed has recently been launched, Antibiotic with the aim to reduce unnecessary duplication of Pharmaceutical agents such as nanospheres, liposomes, and immunoglobulins for example have been developed which can alter the pharmacodynamics of a compound, act as carriers for sustained or controlled drug release, and for immunization therapy.

Introduction The Chilika lagoon situated in the eastern coast of India within 85º05' and 85º38' east longitude and 19º54' North latitude near Bay of Bengal and one of the biggest estuarine-style brackish water lagoon in Asia and the greatest migratory waterfowl wintering area on the Indian subcontinent (Suresh et al., 2018). In the monsoon & summer time, the lagoon's size varies between 1165 and 906 sq. km. The total catchment area of the lagoon is 4,406 sq km, of which 32% is made up of the Mahanadi delta and 68% is western catchment (Bengtsson et al., 2012). The lake has a distinctive combination of freshwater, brackish, and marine ecosystems with estuary characteristics. In 1981, Chilika Lake was chosen as the India's first "Ramsar site" and it considered as one of the biodiversity hotspots of the nation (Kumar et al., 2012). The lake is very productive and has abundant fisheries resources. Over 0.15 million fishermen live around the lagoon and rely on its productive fishing grounds for their livelihood (Suresh et al., 2018). The lake area is home to certain uncommon, endangered and IUCN threatened species.

The integration of bioinoculants and nanoparticles presents a promising strategy for enhancing sustainable agricultural practices, yet significant challenges impede their widespread adoption. Limited awareness among farmers regarding the benefits and applications of these technologies is a critical barrier, emphasizing the need for targeted educational initiatives. Additionally, the high cost of nanoparticle and bioinoculant formulations restricts accessibility, particularly for small-scale farmers. Standardization and quality control protocols are crucial to ensure consistent efficacy, as variability in bioinoculant formulations can compromise their effectiveness. Regulatory frameworks remain complex, further hindering commercialization and large-scale implementation. Environmental concerns are also prevalent, given the potential toxicity of nanoparticles like zinc oxide and silver nanoparticles, which may disrupt soil microbial communities. Despite these challenges, future prospects for bioinoculants and nanoparticles are promising. Integrating these technologies into sustainable farming practices can reduce the reliance on synthetic fertilizers and pesticides, as seen in the use of Azospirillum brasilense encapsulated in chitosan nanoparticles to enhance drought tolerance in maize. Furthermore, the application of Pseudomonas putida encapsulated in alginate nanoparticles has effectively mitigated heavy metal stress in tomato plants. Precision agriculture, employing data analytics and remote sensing, can optimize the targeted delivery of bioinoculants and nanoparticles, maximizing crop productivity and nutrient uptake. However, comprehensive in vivo studies are essential to assess long-term efficacy and environmental impact under field conditions. Future research should focus on developing ecofriendly nanomaterials, refining controlled-release systems, and conducting

Introduction

Advances in food preservation methods have supported the journey of food science from that of a cookery science to a fast growing industrial technology. Food preservation knowledge deals with the way to prevent spoilage and maintenance of quality in foods. Spoilage is the deterioration in quality brought about by inherent biochemical and chemical changes, as well as microbial metabolism during food storage. Basic principles of food preservation (Frazier et al., 2008) remains the same, but newer methods for achieving the goal is added through research fuelled by a consumer demand for fresh-like foods with convenience and safety. Scientific information on novel processing methods are beautifully compiled (Cullen et al., 2011; Da-Wen Sun, 2014; Tokuþoðlu and Swanson 2015; Doona, 2018).

In-depth knowledge of physiological activities of micro-organisms in presence of preservation agents is essential for preparation of safe foods with prolonged shelf life. Besides this, it is necessary to understand the effect of food constituents on microbial resistance against inactivation agents, to identify critical dose requirement. This profound knowledge would also help to understand kinetics of microbial inactivation and to develop mathematical models suitable for a realistic design of process parameters. For better appreciation of the following sections, readers are encouraged to have fundamental knowledge in microbiology and food processing. The sections in this chapter cover advanced microbiological knowledge pertaining to the preservation aspects of food.

Animal husbandry programmes have been run through the State schemes. Each State has to evolve its own breeding policy deciding on choice of breed, cross breeding strategy, optional mixture of animals of different breeds required, breeding goals in terms of expected genetic progress to be achieved, specific breeding programmes and the control measures that should be adopted to achieve the desired genetic gains in the population.
General parameters in the breeding policy formulated by various States are
• Indigenous milch breeds such as Shaiwal, Red Sindhi and Gir, should be selectively developed for dairy traits in their native tracts.
• Indigenous dualpurpose breeds such as Hariana, Tharparkar, Rathi, Kankrej, Gaolao, Ongole Deoni etc. should be developed selectively in their native tracts for dairy and draft traits.
• Indigenous draft breeds like Kangayam, Hallikar, Khillari, Amrit Mahal etc. should be developed selectively for draft traits in their native tract.
• Non-descript cattle will be bred with exotic semen to produce cross breed with Holstein Friesian or jersey and maintaing 50% exotic impenitence. In some States Red Sindhi, Tharparkar and Hariana have also been used upgrading non-descript cattle.
• Development of indigenous breeds
• To develop indigenous breeds Government of India has initiated three schemes namely National Project for Cattle and Buffalo Breeding, Central Herd registration scheme, Central Cattle Breeding Farms.
• Central Herd Registration Scheme
• For identification and location of superior germplasms of cattle and buffaloes, propagation of superior genetic stock, regulating sale and purchase, help in formation of breeders societies and to meet requirement of indigenous bulls in the different parts of the country. Government of India

Cattle Breeding Policies and Programmes in the Planning Process

It is necessary to see how the issues relating to Cattle Breeding have been dealt with in the successive Plan periods starting from the First Five Year Plan. It is seen that, while the issue of surplus cattle has been handled in different ways in the various Plan documents, some discussing this issue at great length and other remaining completely silent on the subject, other issues such as cattle development, genetic improvement and breeding etc., have also received varying degrees of emphasis in the different Plans. 

Introduction

The concept and impetus for IPM grew out of the discontent with using a purely insecticidal approach to insect control in many areas in the mid of twentieth century. Excessive use of pesticide and growing needs of people has triggered several consequences such as resistance and resurgence of pests, destruction and elimination of natural enemies, effect on non target organisms, environmental pollution, disruption in food cycle and food web and residues in food commodities and pesticide poisoning. Less knowledge about biocontrolagents/natural enemies and their non-availability at right time is the key hurdles for farming communities to adopt the IPM practices on their farm. However, pesticides are still a significant component of many control programs and their negative environmental and health impacts have stimulated the research to develop alternate pest management strategies, such as biological control (BC) and other compatible, safe and sound control practices. Some remarkable early successes for both the control of insect pests and weeds (e.g. the cottony cushion scale Icerya purchase (Maskell) by the vedalia beetle Rodolia cardinalis (Mulsant) in California; the prickly pear cactus Opuntia stricta (Haw.) by the moth Catoblastis cactorum (Berg) in Australia certainly were an impetus for further research in BC field. Although the levels of success have been variable, biological control is still considered as an important means of pest control, either alone or as component of integrated management programs (Jervis, 2005; Wajnberg et al., 2007).