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After preparing the desired DNA molecule using E.coli, the plasmid can be transferred into Agrobacterium by this method. Efficiency is low (~10³ transformants per mg of DNA) but the method is reliable and very rapid. This method also eliminates much of the plasmid rearrangement that occurs during triparental mating.

Procedure

Grow an Agribacterium strain containing an appropriate helper Ti plasmid in 5 ml of LB medium 16 hours at 28ºC.

Add 2 ml of the culture to 50 ml LB medium in a 250 ml flask and shake (~250 RPM) at 28ºC until an OD₆₀₀ of 0.5 to 1.0 is obtained.

Chill the culture on ice. Centrifuge the cell suspension at 1000 g for 30 minutes at 4ºC.

Discard the supernatant. Resuspend the cells in 1 ml of 20 mM CaCl₂ (ice cold). Dispense 100 mL aliquots into pre-chilled 1.5 ml tubes.

Add about 1 µg of the desired plasmid DNA (pure or crude) to the cells in each tube.

Fermented foods are food substrates that are produced due to the cumulative activity, usually of edible microorganisms, whose enzymes, particularly amylases, proteases and lipases hydrolyze the polysaccharides, proteins and lipids to non-toxic products with flavours, aromas and textures pleasant and attractive to the human consumer. The role played by fermentation during food processing helps in – enrichment of human dietary through development of wide diversity of flavours, aromas and textures in food; preservation of substantial amounts of food through lactic acid, alcoholic, acetic acid, alkaline fermentation and high salt fermentations; enrichment of food substrates biologically with vitamins, protein, essential amino acids, essential fatty acids and antibiotics; detoxification during food fermentation processing and reducing the cooking times and fuel requirements.

It is an old practice that during the flourishing period, the fishes specially trash fishes are caught from their natural habitat and a bulk of them are preserved to make available for further use. Traditional fish preservation techniques are mainly based on combination of salting, drying and smoking. These techniques have allowed more widespread consumption of fish. Sun drying is most ancient method of fish preservation. Drying removes moisture from tissues and help to arrest bacterial and enzymatic putrefaction.

Fermented fish have, for many years, been considered as a Southeast Asian product. These products are highly salted and fermented until the flesh is transformed into simpler components. Fermentation of fish is a product in which the identity of the fish is maintained to large extent. Fermented fish products as fish sauces, fish paste, or salted fish have been consumed since ancient times. Because of poor roads and other methods of transport, the provision of fresh fish to potential inland consumers was impossible, and this encouraged fermentation as a preservation technique. In Southeast Asian countries such as Thailand, Kampuchea, Malaysia, the Philippines, and Indonesia, the use of fermentation as a preservation method for fish has been of great value since earliest times. In the countries of northern Europe, fermented fish products are used mainly as condiments, whereas in Southeast Asia, various fish products are regarded as staples. In India and Sri Lanka, pickled and in Colombo, cured fish have been known for many years

The traditional fermentation process is an uncontrolled system and is usually carried out in a quite unhygienic environment. As the process of fermentation is brought about by the microbial activity so in the traditional system of fermentation there is every possibility of inclusion of microorganisms which is not desired in or that can cause spoilage to the finished product. Although no incidents of food poisoning has been reported due to consumption of fermented fish there are also no reports of any clinical investigations in this regard. A study on the microflora of such a fermented fish product is useful to understand -how safe is the product for consumption. The diverse groups of microorganisms that are present in the fermented fish products will provide information about their characteristic nature -beneficial or pathogenic, their contribution towards production of flavour and aroma, etc. The environment, however, is the key factor for the so called ‘microbial diversity’ among different fermented fish products round the globe. Moreover, the aquatic habitat, processing technique and of course, the handling during processing are the other factors that equally make a way for the introduction of a variety of microorganisms in the finished product. A study on different curing stages i.e. fresh, semi-cured and fermented, is therefore necessary to understand the pattern of microbes and become evident the role played by the microbes during fermentation.

India is blessed with a coast line of 8118 km with an Exclusive Economic Zone (EEZ) of 2.02 million square km and a continental shelf of 0.506 million sq. km. The inland water resources includes 191024 km of rivers and canals; 2.05 million ha of reservoirs; 2.254 million ha of ponds and tanks; 1.3 million ha of oxbow lakes and derelict waters; and 1.24 million ha brackish waters. India has an estimated fish production potential of 8.4 million tons, of which the marine sector forms 3.9 million tons and the inland sector 4.5 million tons. As against this, the current fish production is 5.96 million tons i.e., 2.89 million tons from marine sector and 3.07 million tons from inland sector (Vannuccini, 2003). The per capita fish availability in India is 4.7 kg/year (Laurenti, 2002).

In 1908, Metchnikoff, the father of modern Immunology, wrote “Prolongation of Life” and created the incredible ongoing revolution known as the “Probiotic Revolution”. He proposed that “Lactic Acid Bacteria” (LAB) could render a great service in the fight against intestinal putrefaction and might postpone and ameliorate old age. Till then today, some 95 years later, dozens of the worlds most brilliant minds carry on the astonishing work that has scientifically been proven to generate restorative and healing effects in the body.

In the late 19^th century, microbiologists identified microflora in the gastrointestinal (GI) tracts of healthy individuals that differed from those found in diseased individuals. These beneficial microflora found in the GI tract were termed probiotics. The term ‘Probiotics’ is originally a Greek word meaning ‘for life’. Parker (1974) for the first time used this word. He defined Probiotics as “organisms and substances, which contribute to intestinal microbial balance”. But the most accepted definition of Probiotics was given byFuller (1989). He redefined Probiotics as “a live microbial feed supplement, which beneficially affects the host animal by improving its microbial balance”. Fuller’s definition since been broaden to state “a probiotic is a mono or mixed culture of live microorganisms which, when applied to animal or man, affect the host beneficially by improving the properties of the indigenous microflora”. This definition stresses the importance of live microorganisms that improve the health status of either man or animal and that occur in the mouth, gastrointestinal tracts (GIT), or upper respiratory or urogenital tracts (Havenaar et al, 1992).

To understand how probiotics work, it is important to understand a little about the physiology, microbiology of GI tract and the digestive process. The digestive process begins as soon as food enters the mouth and to stomach, the microbes present in the GI tract have the potential to act in a favourable, a deleterious or a natural manner. Microbes in small intestine and in large intestine complete the digestion process.

“It is unbelievable that such a tiny streamlet, as was our initial test trial with the first bacterial starters exactly 40 years ago, grew to a big river for an important industry. Who would have believed then that the use of starter cultures would be a matter of course today” (Niinivaara, 1994). The idea of inoculating Lactobacillus into dry sausage material was introduced by Jensen and Paddock in 1940(US Patent 2,225,783) with the aim to reduce the ripening time as well as ensuring the quality and aroma of dry sausages. The first LAB meat starter culture, introduced as a pure culture of Pediococcus cerevisiae, was developed in 1955 in the USA by Niven et al. (1955). At the same time, Niinivaara (1955) applied Micrococcus M53 to dry sausage production in Europe. The work of Niinivaara was continued byNurmi (1966) who combined micrococci with Lactobacillus plantarum. Today, several companies provide Lactobacillus sp., Pediococcus acidilactici, P. pentosaceus, Staphylococcus xylosus or S. carnosus strains in pure cultures or their mixtures for fermentation of meat (Hammes et al. 1985). A starter culture may be defined as a preparation or material containing large numbers of variable microorganisms, which may be added to accelerate a fermentation process. Being adapted to the substrate, a typical starter facilitates improved control of a fermentation process and predictability of its products (Holzapfel, 1997).

Introduction

Livestock sector plays a significant role in the Indian economy, particularly for the welfare of rural population of India. The importance of livestock in India's economy can be gauged from the fact that 90 million farming families rear over 90 million milch animals. Of the total household in the rural areas, about 73 percent own some form of livestock, three-quarter of which are reared by small and marginal farmers.

Today, the overall growth rate in livestock sector is steady and is around 4-5%, despite the fact that investment in this sector is not substantial. This is possible due to the egalitarian distribution of the livestock as compared to land among landless labourers, small and marginal farmers thus resulting in balanced development of the rural economy particularly in reducing the poverty especially among the weaker sections of the society. Livestock also plays an important source of employment in rural India, especially for women. The rural woman plays a significant role in most of the animal husbandry operations related to feeding, breeding, management and healthcare of the livestock.

Livestock plays an important and vital role in providing nutritive food to families both in rural and urban areas. Since the dawn of civilization, mankind has been utilizing different animal species for a variety of purposes, viz., production of milk, meat, wool, egg and leather, draught power, companionship, entertainment, research experimentation, sports, security, etc. Livestock wealth is deemed to be as the oldest wealth resource for mankind and was once a symbol of economic status in the society. This is one sector where poor contributes to the growth directly instead of getting benefit from growth generated elsewhere. The effect of livestock sector growth on poverty is anecdotal, yet scattered evidence on livestock's contribution to income indicates that growth in livestock production is pro-poor.

India has been a welfare country since Independence and the primary objective of all governmental and non-governmental endeavors has been the welfare of its millions people. The policies and programmes should be designed with the aim of alleviation of rural poverty which has been one of the primary objectives of planned development in India. It was realized from pre-independent rural development efforts, that a sustainable strategy of poverty alleviation must be based on increasing the productive employment opportunities in the process of growth itself. Elimination of poverty, ignorance, diseases and inequality of opportunities and providing a better and higher quality of life were the basic premises upon which all the plans and blue-prints of development should built.

Rural development implies both the economic betterment of people as well as greater social transformation. In order to provide the rural people with better prospects for economic development, increased participation of people in the rural development programmes, decentralization of planning, better enforcement of land reforms, greater access to credit, etc proper planning is envisaged.

Initially, main thrust for development was laid on agriculture industry, communication, education, health and allied sectors but later on it was realized that accelerated development can be achieved only if governmental efforts are adequately supplemented with direct and indirect involvement of people at the grass root level.

The livestock sector in India is experiencing fast growth in the present era and plays an important role in the Indian economy. Livestock contributes nearly 25% to the agricultural sector output and growing at an annual rate of 4.3% higher than the growth in agricultural sector as a whole (2.8%). Milk production that had been hovering around 20 million tones in 1960’s increased to 102 million tonnes in 2007-08. The egg production also increased from 0.1 billion to 1.0 billion in the last 25 years. Besides production of milk, meat, etc livestock make important contributions to crop production by supplying draught power and dung manure.

Animal husbandry in India is largely a rural activity. Rapid growth in livestock production is desirable not only to sustain agricultural growth, but also to reduce rural poverty especially when a majority of the land holdings are small. Fifty-eight percent of rural households have land holding of less than 2 ha and another thirty two percent have no access to land. Number of these households is likely to increase due to further fragmentation of land holdings. Livestock are thus an important source of income for small farmers, marginal farmers and landless agricultural labourers in the rural areas of the country. Products like milk and eggs are steady source of cash income, and live animals are important natural assets for the poor, which can be easily liquidated for cash during emergency. Smallholders and landless together control 75% of the country’s livestock resources, and are capable of producing at a lower cost because of availability of sufficient labour with them. These smallholders earn nearly half of their income from livestock sector.

Cooperative

A cooperative is a duly registered association of persons, with a common bond of interest that have voluntarily joined together to achieve a lawful common social or economic end, and making equitable contributions to the capital required and accepting a fair share of the risks and benefits of the undertaking in accordance with universally accepted cooperative principles.

Cooperative may be defined as an autonomous association of persons united voluntarily to meet their common economic, social, and cultural needs and aspirations through a jointly-owned and democratically-controlled enterprise. It is a business organization owned and operated by a group of individuals for their mutual benefit.

A cooperative may also be defined as a business owned & controlled equally by the people who use its services or who work at it.

The basic objective of every cooperative is to provide goods and services to its members and thus enable them to attain increased income and savings, investments, productivity and purchasing power and promote among them equitable distribution of net surplus through maximum utilization of economies of scale, cost-sharing and risk-sharing without however, conducting the affairs of the cooperative for missionary or charitable purposes.

Audio-visual aids may be defined as any device used to aid in the communication of an idea. An audiovisual product is any audiovisual item such as still photography, motion picture, audio or video tape, slide or film strip, that is prepared singly or in combination to communicate information or to elicit a desired audience response. Even though early aids such as maps and drawings are still in use, advances in the audiovisual field have opened up new methods of presenting these aids, such as videotapes and other equipments which allow more professional and entertaining presentations to be presented.

Such aids assist the teacher in communicating information among the learners leading to change in skills, attitudes, knowledge, understanding and appreciation. These aids stimulates the sensory organs like ears, eyes, etc and facilitates quick understanding of the message by the audience. The effective use of these aids depends upon the skillful and imaginative approach of the teacher. Hence, it is necessary that extension worker should know what type of aids will be more suitable for a particular teaching objective. These aids may be used for both literate as well as illiterate people.

The instructional devices through which the messages can only be heard are known as Audio Aids. The instructional devices through which the message can only be seen are known as Visual Aids. The instructional devices through which the message can both be heard and seen simultaneously are known as audio-visual aids.

The basic aim of extension system is to bring about a change in the behaviour of the people with whom the work is done. Change in behaviour pertains to change in knowledge, skills, attitude, understanding, etc about a particular technology which we want to diffuse. For this purpose, the extension workers essentially function as teachers to bring about adoption of new technologies by making regular contact with livestock owners through various means. He visualizes the problems of rural community in proper perspective and organizes the meaningful learning situation for effective learning. It calls for appropriate use of different extension teaching techniques. The desirable changes in the behaviour of learners depend upon the skill of the extension worker as teacher in choosing the various extension teaching techniques which are found to be most effective in the relevant situations.

The main job of the extension worker is to bring about behavioural changes in the rural people. A number of extension methods are required to perform such behavioural changes among the rural people which form the tools of the extension workers. These tools are called extension teaching methods. Extension teaching methods may be defined as the devices used to create situations in which communication can take place between the extension worker and learner. The effectiveness of various teaching methods depends on the combination of the same. Similarly, the adoption of innovation is positively related to the combination of different extension teaching methods, at different stages of the learning process.

One of the major constraints in the efforts to modernize and efficient restructuring of the animal husbandry practices is the delay in taking the results of research to the farming community and in persuading them to adopt new, profitable and locally relevant technologies. The very need of the extension work is to bridge the gap between what is known to the scientists in the laboratories and what is actually being practiced by the farmers in the field. In other words, the major goal of extension machinery is to accelerate the processes of communication and to motivate rural people or livestock owners for adoption of new and profitable technologies. Hence, it is very important to understand these processes of how the farmers get such technologies and how they adopt them.

Communication

The term communication is originated from the Latin word ‘Communis’, meaning common or establishing commoness. When we communicate, we attempt to establish ‘commonality’ with someone through the message.

Communication is the process by which two or more people exchange ideas, facts, feelings or impressions in ways that each gains a common understanding of the meaning, intent and use of the message (Paul Leagens, 1961).

Rural development programmes are created and maintained for influencing people to make changes in their way of living and of making a living. The existence of such programmes implies that the present situation of the rural people is not what it should and could be, that something different should prevail, and that it is possible through appropriate action to attain a more desirable status for them.

Changes that are important to people are those that help them to meet their needs for biological, economic, social, aesthetic and moral well being. Basically, all programmes for rural development exist to help people to meet their needs. If programmes do this people have their free choices in societies and will not participate for long periods in them. The element of people’s needs, therefore, becomes a central concern of planner for rural development. Hence, the entire process of planning for rural development implies a need for change. But now the question arises that change from what, to what, by whom, where and by what methods?

The society has to find ways to adjust itself so that people meet their needs for an improved economic status, for being more useful citizens and for enjoying a higher degree of social stratification, it must identify these conditions and plan realistically to achieve them. It is the prime concern to identify that what needs do people have ? How can needs be identified? What plan of priority should be set up for meeting people’s needs? What resources are necessary to meet people’s needs? How should resources be organized and directed to help people’s meet their needs? These questions give the key orientation and justification for rural programme planning.

Farming system is an integrated set of activities that farmers perform in their farms with available resources and circumstances to maximize the productivity and net farm income on a sustainable basis. The farming system conceptually is a set of elements or components, i.e., soil, water, crops, livestock, labour, capital, energy and other resources that are interrelated and interact among themselves. At the centre of the interaction lies the farmer exercising control and choice regarding the types of results of interaction. The income of cropping alone from small and marginal farm is insufficient now to sustain the farmers family. A judicious mix of any one or more of these enterprises should complement the farm income and help in recycling the farm residues / wastes. The selection of enterprises must be based on the cardinal principles of minimizing the competition and maximizing the complementary between the enterprises. Of late, the researchers on multidisciplinary approach greatly realized and started developing the various farming systems models in accordance with the agro-eco systems zones.

Farming System thus may be defined as a complex interrelated matrix of soil, plants, animals, implements, power, labour, capital and other inputs controlled in part by farming families and influenced to varying degrees by political, economic, institutional and social forces that operate at many levels. The farming system therefore, refers to the farm as an entity of inter-dependent farming enterprises carried out on the farm. The farm is viewed in a holistic manner and the farmers are subjected to many socio-economic, biophysical, institutional, administrative and technological constraints.

Nearly 50 percent of the world’s population lives in urban areas, it is not possible that food production be adequate for their needs. In some areas of food production surplus, it must be brought to the deficit places where peoples live and work and made available at prices they can afford, otherwise many will remain hungry. This is why marketing is a crucial part of any campaign to relieve hunger. Marketing is the means by which food is brought from the place where it is produced to the people who want to eat it.

Marketing can be a very simple process and it can also be very complex. This depends on what must be done to the farmer’s product to get it from the field to the consumer in good conditions, in an acceptable form and to have it ready when the consumer wants.

Marketing is relatively simple in a village community which produces most of what it needs. Here the producer can sell directly to the consumers coming to his house, or he can attract them by displaying his produce in some central open space in the village which then becomes known as ‘the market’. The total volume of the livestock products sold in this way is still very large, especially in the less commercially developed countries. Direct marketing within the villages is sometimes praised because of its low cost, and also because it does not involve any ‘middleman’ between producer and consumer. But, if farmers sell their produce only to the local consumers, and if their land could produce more than they need, there is no way by which they can take advantage of these excess production potentialities. Similarly, if the consumers are only able to eat what is produced locally, which may confine them to a diet that is monotonous, unsatisfying and very expensive too. This is true of many people’s living in isolated areas, which requires much home processing and still has little nutritional value; and of the many others for whom most meals consist of hand-pounded cereals. Hence, this undernourishment on one side and excess production on the other side leads to the development of marketing concept.

Administration

Term Administration is derived from a Latin word Administaire which means to serve. The word administration is also derived from the English word administracioun, which is in turn is derived from the French word administration, which itself has its root from the Latin word administratio, which means ad (to) and ministratio (give service). The term administration is a more general and comprehensive connotation and may be defined as the universal process of organizing people and resources efficiently so as to direct activities toward common goals and objectives. Administration is a distinct skill and directed towards the guidance, leadership and control of the efforts of a group of individuals towards some common goal.

Administration is the art of guiding, coordinating and integrating policies established for any organizational unit to effectively and efficiently achieve the purpose of the organization.

Taxonomic Classification of Common Livestock Species

A series of terms (kingdom, phylum, class, order, family, genus, species etc) is used to denote the member of a kingdom and each division of the hierarchy contains one or more groups from the next lower division like subphyla, subclasses, suborders, subfamilies, or subgenera etc if required. Further, species may be subdivided into varieties, breeds, or strains. Domestic farm animals belong to either class Mammalia or Aves. The scientific name of an animal comprise of its genus and species names. The first letter of genus name is written in capital letter but not the species name. While writing the scientific name of an animal the genus and species names are underlined when handwritten and always italicized when printed. General characteristics and taxonomic arrangements of common domestic animals have been given below.

Kingdom : Animalia

Members of kingdom Animalia are multicellular, eukaryotic heterotrophs. Adult animals develop from embryos. Most animals ingest and digest their food in some kind of internal cavity.

Body Parts of Farm Animals

It is important to know about the various terms used to indicate different body parts of the animals so that whenever a description of the animal/body part is presented, one could specifically understand it. Some of the terms used to point out different body points of common livestock species have been shown in Figure 2.1.1-2.1.5. Terms which are more frequently used for different livestock species have been depicted in Table 2.1.1.

Animal Genetic Resources

Agricultural biodiversity is the product of thousand years of activity during which humans have sought to meet their needs in a wide range of climatic and ecological conditions. Well adapted livestock have been an essential element of agricultural production systems. For livestock keepers, animal genetic diversity is a resource to be drawn upon to select stocks and develop new breeds. More broadly, genetically diverse livestock populations provide society with a greater range of options to meet future demands, so the wise management of the world’s agricultural biodiversity is becoming an ever greater challenge for the international community. The livestock sector in particular is undergoing dramatic changes as large-scale production expands in response to surging demand for meat, milk and eggs (FAO, 2008).

Animal genetic resources (AnGR) include all animal species, breeds and strains that are of economic, scientific and cultural interest to humankind in terms of food and agricultural production for the present or the future. Domesticated animals are considered to be those species that are bred in captivity and modified from their wild ancestors to make them more useful to humans, who control their reproduction (breeding), care (shelter, protection against predators) and food supply (Diamond, 2002; Mignon-Grasteau, 2005). Only about 40 animal species have been domesticated since the Pleistocene. Common species include cattle, sheep, goat, chicken, duck, pig, horse, buffalo, rabbit, camel, donkey, elephants, various poultry species, reindeer, etc. All these AnGR are vital to the economic development of the majority of countries in the world playing an important role in the subsistence of many communities. Although only a subset of the diversity present in the ancestral species survived in the domestic counterparts, domestic livestock diversity has been continuously evolving.

Livestock farming is an integral part of Indian agriculture system and vital source of livelihood particularly in the rural areas of the country. This sector has been the primary source of energy for agricultural operations (for land preparation, sowing, pulling bullock carts etc.) and major source of animal protein (mutton, chevon, chicken, pork, beef, eggs etc.) for majority of population. India has been habitate of major draught, milch and dual-purpose breeds of cattle as well as buffaloes. Besides their potential our indigenous as well as crossbred animals are unable to produce maximum output due to unavailability of adequate and nutritious feed and fodder throughout the year. The quantity and quality of forage production is directly proportional to the livestock health, production and productivity. There is colossal gap between demand and supply of good quality nutritious fodder. Besides, the availability of lesser area under cultivated fodder crops, the grazing lands also becoming smaller due to the degradation of the land, urbanization, industrial development and increased area under food production to fulfil the demand of increasing human population. To meet the nutritional requirement of such a huge population of indigenous, crossbreds as well as genetically upgraded animals, proper management and conservation of fodder and grasslands is necessary.

Functions of Livestock Housing

The most important purpose of livestock production is to increase the conversion efficiency of feed energy into the livestock products like milk, eggs, meat, wool, hair, hides and skins, draught power and manure (fertilizer). Scientific housing of livestock is necessary in order to get higher productivity from them. A proper housing design/structure should alter/modify the micro-climate inside the house so that animals are least exposed to the extremes of climatic conditions. Moreover, it should also provide better control of diseases & parasites, desirable working conditions for farm staff & better living conditions for the animals, better integration of various routine farm practices, opportunity for higher labour efficiency at farm, protection from predators & thieves, opportunity to express species specific natural behavior patterns and it must not compromise the welfare of the animals. Some of these functions are discussed in subsequent sub-headings.

Origin & Distribution of Cattle

All types of domestic cattle belongs to class Mammalia, subclass Ungulata, order Artiodactyla, suborder Ruminantia, family Bovidae, subfamily Bovinae, tribe Bovini, which includes genus Bos. The aurochs or urus (Bos primigenius) is the probable ancestor of most of the present day Bos taurus and Bos indicus cattle. The humped Bos primigenius namadicus were the forerunner of today’s zebu cattle while humpless Bos primigenius primigenius gave rise to modern European cattle. Both category of cattle possess the same number of chromosomes (2N=60). Other related animals in the Bovini tribe include the gaur cattle (Bos gaurus), banteng (Bos banteng), kouprey (Bos sauveli) of South and East Asia, bison (Bison bison) of North America, European bison (Bison bonasus) and the yak (Poephagus mutus) of central Asia. According to Payne & Hodges (1997), domestication of aurochs (Bos primigenius ) took place first probably on the lower mountain slopes in the region of western Asia known as the ‘fertile crescent’. They suggested that the first domestic cattle were humpless (Bos taurus) Hamitic Longhorn type and were domesticated around possibly 9000 BP (years before the present i.e. 1950). About 2000 years later the humpless (Bos taurus) shorthorn type evolved or was developed in the region and these two types of humpless cattle are believed to the ancestor of Bos taurus. Humped (Bos indicus) zebu cattle were domesticated in the northwest arid areas of the Fertile Crescent around c. 5000 BP.

Origin and Domestication of Goat

Goat is a cloven-hoofed, hollow-horned ruminant which is comparatively smaller in size and belongs to class Mammalia, order Artiodactyla, family Bovidae and genus either Capra (2N=60) or Hemitragus/tahrs (2N=48). The distinction between these two genera was previously based on horn structure but the distinction has been confirmed genetically. The domestic goat (Capra hircus/Capra aegagrus hircus) belongs to the genus Capra and has possibly developed from five wild ancestors. On the basis of available information, it is most likely that bezoar is the main ancestor of most domestic goats. However, there is slight likelihood that Markhor has been involved in the parentage of some of the Indian goat breeds.

Evolutionary biology indicates that domestication of goats started during Neolithic age and it was perhaps the first ruminant and first animal after dog to be domesticated by the man around 9000-7000 BC. Domestication was associated with three of the oldest civilizations namely- (i) the Nile in northeast Africa, (ii) the Tigris-Euphrates in west Asia, and (iii) the Indus in the Indian subcontinent. Archaeologists indicate that the goat was first domesticated in the “Fertile Crescent” (landmass which extends between the Black and Caspian seas along the coast of Palestine and forms arc like a quarter moon towards the Persian Gulf) of the eastern Mediterranean. Since their domestication, goats remained associated with variety of functions in various aspects of human life including food, employment, economics, folklore and religion.

Origin and Domestication of Sheep

The domesticated sheep belongs to phylum-Chordata, class-Mammalia, order- Artiodactyla, family - Bovidae, sub-family - Caprinae, genus - Ovis and species- aries. They are hollow horned, cloven-hoofed ruminants of relatively smaller size. Sheep plays an important role in Indian agrarian economy as it is having multi facet utilities like meat, wool, skin, manure and to some extent milk and transport also. Marginal and sub-marginal lands of arid and semi-arid tropics are well adapted by sheep. Among the different wild species, Asiatic (Ovis orientalis) species of mouflon is considered to be the ancestor of present day sheep. Sheep were domesticated by man at around 9000 BC. The reference of role of sheep in the economy of mankind is found in the history of civilization of Mesopotamia, Mohen-jo-Daro and Harappa in Northern India.

Origin and Domestication of Pigs

Pigs are nonruminant, even-toed ungulates and belong to suborder Suina which includes hippopotamuses, peccaries and pigs. All true pigs are members of the family Suidae which includes five living genera namely Sus, Babirousa (babyrusa), Hylochoerus (giant forest hog), Phacochoerus (warthog), and Potamochoerus (bushpig and red river hog) that can be called as pigs. European breeds are believed to be descendants of the European wild pig Sus scrofa and Asiatic or oriental breeds originated from the Eastern Asiatic banded pig, Sus vittatus a successor of Sus crystatus.

The group of animals constantly used in laboratories for general research in all discipline of science is called as laboratory animals. Commonly, it includes rats, mice, rabbits and guinea pigs. However, under certain circumstances, additional animal species like hamsters, non-human primates, amphibians, fowl, sheep and pigs can also be included. This term incorporates traditional laboratory animals, farm animals, wildlife and aquatic animals. Nontraditional animals, inclusive of invertebrate species are also included where they are relevant to the unit’s mission. Generally, these animals come under vertebrate but broadly laboratory animals may be classified in two major groups i.e. laboratory mammals and non-mammalian laboratory animals. The laboratory mammals include mouse, rat, guinea pig, rabbit, cat, dog, ferret, gerbil, bats, hamster, mink and monkey (Rhesus). Beside these mammalians the large domestic animals/livestock are also used as laboratory animals (horse, cow, sheep, goat etc) for fulfilling the demands of various laboratories. Poultry is also used as non-mammalian laboratory animals and Specific Pathogen Free (SPF) chicks are at utmost demand for production and testing of biologicals. The other non-mammalian laboratory animals includes reptiles (tortoise, lizzards and snakes), insects (cockroach, drosophila, house fly, house cricket, locusts, tsetse fly, stablefly etc) pisces (fishes and snails) and amphibians (toad and frog). Laboratory animals have been used extensively for basic research in drug and bacteria screening, toxicology, antibiotic sensitivity testing, animal models of diseases and production/testing of biologicals. Laboratory mammals are also valuable for diagnosis of infectious diseases in man and domestic animals. In this chapter the most commonly used laboratory animals i.e. mice, rats, guinea pigs and rabbits will be discussed.

Equines are herbivorous monogastric animals. Though, simple-stomached, they can digest considerable amount of fibrous feed in the caecum and are called as hindgut fermenters. Equine includes horse, donkey, zebra and mule which are used as work and pack animal. All three species of equine belongs to the family Equidae. The scientific name of Horse and Donkey is Equus caballus and Equus asinus, respectively. The horse was probably last farm animal domesticated by the man. Horses appear to have been domesticated first in central Asia or Persia, more than 3000 years ago.

Origin and Distribution of Camel

A camel is an even-toed ungulate within the genus Camelus of the family Camelidae bearing distinctive fatty deposits known as “humps” on its back. There are two species of camel, viz. Camelus dromedarius (single humped dromedary) having habitat in dry hot arid lands of Africa and Asia, and Camelus bacterianus (double humped or bactrian) habitat of cold arid lands of Central palaerctic (a zoogeographical region consisting of Europe, Africa north of the Sahara, and most of Asia north of the Himalayas) region. Family Camelidae also includes other species which are camel like mammals. Those are Lama (Lama glama), Alpaca (Lama pacos), Vicuna (Vicugna vicugna) and Guanaco (Lama guanicoe). These four species are termed as new-world Camelidae having habitat in South America. The Indian camel belongs to Camelus dromedarius. A very small numbers of double-humped camels are also available in Ladakh area of Jammu and Kashmir.

Camel is an important component of desert eco-system. It is capable of traveling long distance in hot, arid and sandy regions carrying men, material and providing energy to agricultural operations. They provide milk, meat, hair and used as work animals. It is believed that Camels were domesticated during the period from 3000 to 2500 BC. Average life expectancy of a camel is 40-50 years. The height at the shoulder and hump of a fully grown adult camel is 1.85 and 2.15 metres, respectively. Camel can run at a speed of up to 65km/h for a short distance and can sustain a speed of up to 40km/h for long distance.

Poultry sector in India has grown from a backyard activity into a major commercial activity in four and half decades. Poultry is one of the fastest growing subsectors of agricultural sector in India. Within the poultry sector, broiler production is growing faster than egg production and about 66.7% of the total output (in value term) comes from the poultry meat sector and only 33.3% from egg production (FAO, 2008). India ranks fifth in chicken and sixth in duck population. Presently, India is world’s third largest egg producer and the fifth major producer of chicken meat (FAO, 2010). Poultry is today the major source of meat in India. As per an estimate about 2 million people are employed, fully or partly, along the poultry value chain and that an increase in annual per caput availability of one egg or 50 gm or poultry meat generates about 20,000 to 25,000 full time jobs (Ciamarra & Otte, 2009). Distribution of chicken and duck population as well as poultry meat and egg production among different regions, countries of the world and among different Indian states has been already discussed in chapter one of this book.

The biotic component of earth is continuously and significantly affected by the abiotic components. Physical factors of the environment (temperature, humidity, rainfall, wind, solar radiation, light, atmospheric pressure, etc) surrounding the animal have influence on the production, reproduction and welfare of the animal. Livestock performance is best when environmental factors are optimum which is difficult to achieve due its variable nature. To this complex livestock-climate interaction, animal responds favorably by undergoing ‘adaptation’ or unfavorably by being under ‘stress’. Type and magnitude of stress due to physical environment depends on climatic factors of a particular area, animal adaptability, breed characteristics and managemental interventions by humans to minimize the losses. To understand the physical environment of livestock frequently used terms are mentioned below

Globally livestock sector is changing rapidly in response to globalization and increasing demand for quality foods of animal origin. Animal husbandry is the backbone of rural economy of our country. The value of output from livestock sector is about 29 percent of the total value of output (GDP) of the agriculture and allied sectors. The livestock sector has been contributing over 5% to total GDP. India ranks first in milk production which accounts for more than 15 percent of total world output and 40 percent of Asia’s total production. It continues to grow at about 4 percent per annum far exceeding the global average of 1-2 percent. Further, the manure from animals provides a good source of organic matter for improving soil fertility and crop yields. The gobar gas from the dung is used as fuel for domestic purposes. The surplus fodder and agricultural by-products are gainfully utilized for feeding the animals. Almost all draught power for farm operations and transportation is supplied by bullocks. Indian animal husbandry practices are based on minimal use of off-farm inputs and on management practices that restore, maintain and enhance ecological harmony. This principle also holds good for organic farming. The primary goal of organic agriculture is to optimize the health and productivity of interdependent communities of soil life, plants, animals and people (NOSB, 1995). Organic agriculture is an ecological production management system that promotes and enhances biodiversity, biological cycles and soil biological activity.

1.1 Introduction

Since 1990s optoelectronics and fiber optics technology underwent significant advances due to innovations in the telecommunications, semiconductor and consumer electronics sectors. The developments of compact disk (CD) players, laser printers and the high performance and reliable optical fiber communication are the result of these advances. The optical fiber sensor technology is a direct outgrowth of the revolutions taken place in optoelectronics and fiber optic communication industries. Many of the components associated with these industries are used for optical fiber sensor applications. In the beginning the main hurdle in the development of sensors was the cost of components such as optical sources and detectors. With the time, components prices are falling and the improvements in fiber, laser and photodetector technology have taken place. It is expected that as the time progresses the optical fiber sensors will replace the conventional devices for the measurements of various physical, chemical and biological parameters such as rotation, acceleration, electric and magnetic fields, temperature, pressure, acoustics, vibration, position, strain, humidity, viscosity, pH, glucose, heavy metals, gases, viral infection, pollutants etc.

2.1 Introduction

In a fiber optic sensor the heart of the sensor is an optical fiber. An optical fiber in its simplest form is a cylindrical symmetric structure consisting of a central ‘core’ glass/plastic of uniform refractive index and of diameter 4-600 µm. It is surrounded by a ‘cladding’ glass/plastic of slightly lower refractive index. To provide the mechanical and environmental protection to the fiber, the cladding is covered with an external plastic coating (seeFig. 2.1). The light in the fiber propagates by bouncing back and forth from the core-cladding interface. The design of the fiber optic sensor depends critically on the choice of optical fiber. Telecommunication fibers are inexpensive but may not be the right choice for sensors. Some of the fiber optic sensors dictate the use of application-specific optical fibers. In this chapter we shall first describe ray analysis for the light propagation in the fiber. The modes propagation, numerical aperture of the fiber, fiber characteristics and different kinds of optical fibers used for sensing will be described later.

3.1 Introduction

Optical sources are a key element in many fiber optic systems. This component converts the electrical signal into a corresponding light signal that can be injected into the fiber. Several factors such as wavelength, source output and spectral bandwidth are important when selecting light source to achieve the best performance of the sensor. The optical sources used for sensors range from hot filament lamps to semiconductor lasers. These sources can be placed into two groups - incoherent and coherent sources. The tungsten lamp and light-emitting diode (LED) are some of the incoherent sources while the lasers come in the category of coherent sources. The LED is normally preferred if the multimode fiber is used for the sensor. If the optical power requirement is high then the laser with multimode optical fiber is used. For the sensors based on interference phenomenon, coherent sources with single mode fibers are used. In the case of distributed sensing, short pulse high power lasers are used. In a laboratory demonstration experiment, a visible laser such as a simple He-Ne laser may be the preferred source while in commercial applications a compact and rugged source such as laser diode or an LED is preferred. Before using the source it is very essential to know its maximum power output, wavelength of emission, linewidth, radiation pattern and modulation bandwidth. The purpose of this chapter is to provide the physics of some of the light sources and their important operating characteristics that are used in sensors.

4.1 Introduction

Apart from an optical source, the optical detector is another important component of the optical fiber sensor. The optical detector converts the optical signal to an electrical one. The signal is then processed either to record or display. There are various kinds of optical detectors but the commonly used in sensors are semiconductor diodes. In some of the cases photomultiplier tube has been used. In this chapter we shall describe the basic principles of optical detectors and their operational characteristics.

5.1 Introduction

Evanescent field absorption spectroscopy is a powerful well-established laboratory technique for chemical analysis. The technique utilizes the evanescent wave penetration at the boundary between two dielectric media in conditions of attenuated total reflection (ATR). One of the media, a thin slab-shaped non-absorbing crystal, is called a waveguide, while the other medium of lower refractive index is the absorbing sample being studied. At each reflection at the boundary of the crystal and the sample, the penetration of the evanescent wave of the guided ray into the absorbing sample gives rise to a reduction of the power propagating in the crystal. The degree of absorption depends on the amplitude of the evanescent field in the sample medium and the number of reflections within the waveguide. The former increases dramatically for incident angles approaching the crystal-sample critical angle while the latter increases with the decrease in the thickness of the waveguide. The number of reflections can also be increased by increasing the length of the waveguide. Thus, to achieve maximum absorption, an unclad optical fiber is well suited for evanescent wave absorption spectroscopy. This is because, unlike the crystal waveguide, the narrow core diameter of the fiber provides a large number of reflections per unit length. Further, the use of an optical fiber to transmit light to a section of its core in contact with an absorbing sample offers the possibility of remote sensing or in-line analysis. These advantages, together with the benefits of simplicity, reliability, flexibility and relatively low cost have motivated a number of research groups all over the world to investigate evanescent wave spectroscopy at the surface of an unclad optical fiber. This chapter will be devoted to the fiber optic sensors utilizing evanescent field absorption spectroscopy.

6.1 Introduction

There are a variety of intrinsic and extrinsic fiber-optic sensors which use absorption spectroscopy indirectly for the detection of various chemical species. These include pH, metal ions, oxygen in water etc. In such sensors a suitable reagent is immobilized on a solid support. The support can be the core of the fiber or some optical material attached at the end of the fiber. Further it is optically transparent to allow transmission of the light signal from the interface and should remain inert to the chemical reaction being analyzed. The analyte (or the chemical species) when comes in contact of the reagent affects the absorption properties of the reagent. In other words, in these sensors, the reagent acts as a chemical transducer for analytes that are not directly measurable by optical techniques. Following conditions for the use of reagents in sensors must be met:

7.1 Introduction

There are numerous wavelength dependent phenomena that may be used to modulate the wavelength of incident light source. These are wavelength dependent absorption, luminescence, dispersion, interference and scattering. Wavelength modulated sensors use a broadband source, a wavelength modulator or measurand (i.e. analyte), a form of spectrometer (using prisms, gratings or interference filters), a detector (single photodiode or array) and signal processing unit. Silicon photodiode is cheap and can be used for detection but it limits the detection wavelengths to the region 400 to 1050 nm. The sensitivity or the resolution of the wavelength modulated sensor depends on the characteristics of the detector, the inherent resolution of the spectrometer, mechanical stability of the modulation unit and the spectrometer, and the capability of the signal processing unit. However, wavelength modulated sensors do not require any form of referencing like intensity modulated sensors. In intensity modulated sensors, loss in intensity occurs due to connectors and splices, microbending, mechanical creep and misalignment of light sources and detectors due to environmental effect. Further the fluctuations in light source can affect the performance of the sensor. Thus, for intensity modulated sensors one needs referencing to cancel out these losses. In some intensity modulated sensors two wavelengths are used. One wavelength is used to cancel out all of the losses while the second is used to sense the measurand.

Wavelength detection is relatively easy if the spectral changes result from changes in chemical indicator dyes, fluorescence, phosphorescence, or black-body radiation. In these cases the incident and emitted wavelengths are far apart and therefore detection is easy. If the spectral change occurs due to mechanical movement, then the wavelength filtering mechanisms must have sufficient resolvable spectral points. The reasons for using fluorescence in the fiber optic sensors are well known. Fluorescence is intrinsically more sensitive than absorbance and is more flexible in that a great variety of analytes and physical parameters are known to change the emission of particular fluorophores. The fluorescence based sensors utilize the change in the intensity, lifetime or colour of fluorescence as the affected parameter by the measurand. That means, any interpretable observation of fluorescence can be made the basis of a sensor. In this chapter we shall first describe luminescence phenomenon and then various physical and chemical fiber optic sensors based on wavelength modulation and fluorescence phenomenon.

8.1 Introduction

Interferometric or phase modulated sensors have received special attention because these are much more sensitive than intensity modulated sensors and can be used over a much larger dynamic range. The sensors rely on the interferometric technique. Generally, the sensor employs a coherent light source such as a laser and two single mode optical fibers. Light is split and coupled into both the fibers. If the measurand perturbs the optical properties of one of the fibers relative to the other, a phase shift occurs. The change in phase can be measured very precisely. For the measurement a sophisticated electronic processing equipment is needed. Thus the interferometric sensors are expensive compare to intensity modulated sensors. There are four kinds of interferometer configurations: Mach-Zehnder, Michelson, Fabry-Perot and Sagnac interferometers. The Mach-Zehnder, Michelson and Sagnac interferometers are the two beam interferometers while in the case of Fabry-Perot interferometer multiple beam interference occurs. In this chapter we shall first describe interference phenomenon and then different kinds of fiber optic interferometers. In the end we shall describe a number of sensors based on interference phenomenon or phase modulation.

9.1 Introduction

Many physical parameters such as pressure, temperature, electric current etc. can be measured using polarization-modulated sensors. In these sensors modulation of state of polarization of light in the sensing region by an external stimuli takes place. A variety of physical effects influence the state of polarization of light. These phenomena are Faraday effect, electro-optic effect and photo-elastic effect. Polarization modulated sensors, in some cases, offer significant advantages not only over conventional sensors but also over widely used fiber optic interferometric sensors. These sensors require control over the state of polarization of light entering the sensing region of the fiber. Thus, for these sensors, highly birefringent fibers or single mode fibers are used. In this chapter we shall first discuss magneto/electro-optic effects and then shall describe sensors for the measurement of electric current/ magnetic field, voltage, pressure, temperature etc. utilizing polarization modulation.

10.1 Introduction

There are very few frequency modulated fiber optic sensors. This is because frequency modulation of light occurs under a limited range of physical conditions. The principal one is based on Doppler effect. There are few other circumstances under which the frequency of light is modulated. These include luminescence and Raman scattering. Luminescence has been included in chapter 7under fluorescence based sensors. In this chapter we shall focus on the sensors based on Doppler effect and Raman scattering. In particular, we shall discuss the measurement of blood flow velocity, colloidal particle motion and Raman spectrum using optical fibers.

11.1 Introduction

In previous chapters we have described fiber optic sensors based on intensity and phase modulations. These sensors have some problems in practical applications that need to be solved. Some of these are fluctuation of light source power, loss in couplers, scattering and absorption effects that affect measurement performance, temperature drifts and vibrations. In comparison to intensity and phase modulated sensors fiber Bragg grating (FBG) sensors have following advantages:

High resolution

Insensitive to intensity fluctuation because the signal obtained from an FBG sensor is encoded directly in wavelength domain.

Provision of well localized sensing region

Linear output as a function of the measurand

The capability of multiplex many sensors along one fiber

12.1 Introduction

Among a variety of optical evanescent wave techniques, spectroscopies based on the resonant excitation of surface plasmons have matured into a widely accepted surface analytical method used in different fields. Liedberg et al¹ were the first who demonstrated the exploitation of surface plasmon resonance (SPR) for chemical sensing. Since then the SPR sensing principle has received much attention. In this chapter we shall first describe the principle of this technique and then few sensors that have utilized this phenomenon.

13.1 Introduction

Distributed optical fiber sensors are those sensors which monitor a single measurand at a large number of locations or continuously over the path of the optical fiber. The measurand can be a physical or a chemical quantity. The distributed optical fiber sensor generally consists of a common light source, optical fiber to carry light to the sensing probes and a return fiber to carry modulated light to a photodetector. These sensors can utilize any kind of light modulations described earlier. The advantages associated with distributed sensing are reduced number of components and hence the cost, reduced weight and cabling, and electrical passivity. The applications include the measurements of temperature, pressure, strain, current and chemical concentration. If the measurand is monitored continuously along the fiber length then the sensor is called intrinsic distributed sensor. If it is measured at a finite number of locations then the sensor is called quasi-distributed sensor. The quasi-distributed sensor has a number of advantages over intrinsic distributed sensor. In quasi-distributed sensor, spatial resolution is not as important as in intrinsic distributed sensor provided that individual sensors can be clearly resolved. Further, in quasi-distributed sensing, the sensor elements can be tailored in terms of responsivity and range to meet the specific needs of the application. In this chapter we shall describe various kinds of distributed sensing approaches and few optical fiber distributed sensors.

14.1 Introduction

In this chapter we shall describe various simple and easy to fabricate fiber optic sensors. Most of these sensors are based on intensity modulation. Intensity modulated optical fiber sensors have been extensively studied in comparison to sensors based on other modulation techniques. This is because of their several advantages such as simplicity, reliability, flexibility and relatively low cost. Intensity modulated sensors make use of multimode fibers. Multimode fibers have large core and high numerical aperture that make the coupling of light into the fiber easier. The large core is also better suited to propagate high power laser beams. Further, the plastic-clad fused silica multimode fibers are available. Plastic-clad silica multimode fibers have an additional advantage. Their plastic cladding can be removed easily permitting access to the core. The evanescent field in the unclad part has been used for various kinds of chemical and biochemical sensors (see chapter 5). Above all, these fibers are cheap. This chapter is devoted to simple and easy to make both intrinsic and extrinsic type sensors. Sensors utilizing bare-ended fibers have also been described.

Introduction

Impact assessment is the process of identifying the future consequences of a current or proposed action. It is used to ensure that projects, programmes and policies are economically viable, socially equitable and environmentally sustainable. Programme Monitoring and Evaluation (PME) efforts are critical issues in management of agricultural research and development (R & D). In times of fiscal constraint, more emphasis is placed on accountability and programme effectiveness. As a management tool, PME is an integrated approach that incorporates the viewpoints of clientele, policy-makers, researchers and donors to address the intended goals. Public and private support to research will depend largely on efficiency issues and ability to reckon with multiple objectives, ranging from food security to sustainability, poverty alleviation, gender issues, export, etc. The PME of agricultural R & D projects is critical when the intended R&D has multiple outcomes. Monitoring and evaluation (M&E) of development activities provides government officials, development managers, and civil society with better means for learning from past experience, improving service delivery, planning and allocating resources, and demonstrating results as part of accountability to key stakeholders (World Bank, 2004).

Introduction

The acute food shortage was a major challenge in confronting the development of the country after independence with rapidly increasing population. Massive financial assistance of credit and subsidy under various rural development programmes unveiled by government could not yield desired impact on poverty and unemployment. The benefits of farm research did not reach the resource poor farmers. Several researchers indicated that the devolution of democratic decision-making at the village level was lacking.

The major task of bridging the gap between farmers and the scientific knowledge is in the hands of extension workers who should act as “conveyer belts” in communicating appropriate scientific knowledge and skills already available to the farmer in a smooth and acceptable form and in securing the adoption of these improved techniques. They must assess the intentions prevailing in the minds of rural people, their relationships and interactions, their groups, their institutions, their organizations and the culture, which influence the behaviour at large. They need to understand their motives, reactions, receptivity to new ideas, hidden resources of rural people and social and cultural obstacles prevailing in the society with their active participation.

To elucidate the hidden facts from the well-knit social, cultural and behavioral patterns, a family of approach known as “Participatory Rural Appraisal (PRA)” techniques is widely employed to understand the existing farming situations, problems, and opportunities in the rural areas. PRA techniques can also be used in evaluation and impact assessment in agricultural and allied enterprises and projects that can indicate the success and failure to plan for better future.

Logical Framework Approach (LFA) is a framework for designing change process, monitoring progress and evaluating impact. It is a powerful tool for project design, management, assessment, follow up and evaluation of projects. In designing change process, monitoring progress and evaluating impact, ordering the change process in a logical sequence where inputs or resources identify as necessary means for performing activities are very important. Specific activities are then identified because they result in outputs which are necessary to achieve objectives. The two terms i.e.; log frame and logical framework approach are sometimes confused by many. The log frame is a document whereas the logical framework approach is a project design methodology. LFA originally developed by the American Defense Department and was first formally adopted as a planning tool for United State Agency for International Development (USAID) by the end of 1960’s and today it is being used, in one form or other and with different names, by major organisations in the field of development co-operation and private industry. Among these are British DFID, the OECD expert group on Aid Evaluation, ISNAR International Service for National Agricultural Research, Germany’s GTZ and the Inter-American Development Bank. It has also been widely adopted by NGOs, though not without reservations and concerns by some. In the 1990s it was often mandatory for aid organisations to use the LFA in their project proposals.