eChapters

1 What is the best time to sow onion seeds in Northern Hilly regions?

 a) January to February b)  March to April

 c) May to June  d) September to November

 2 Which type of soil is best for onion cultivation?

 a) Sandy loam    b)  Clay loam

 c) Alluvial soil  d) Both a and b

 3 What should be the pH range for onion cultivation?

 a) 4.4-5.0    b)  5.5-6.5

 c) 6.0-7.5    d)  6.5-7.5

1. What is the main negative impact of physiological disorders on root crops?

a) Deterioration of quality and visual characteristics

 b) Nutritional imbalance

 c) Decrease in yield

 d) None of these

2. Forking is a common physiological disorder of which root crop?

a) Radish

c) Beetroot

b) Carrot

 d) Turnip

1 Which soil is most suitable for bottle gourd cultivation?

 a) Sandy loam soils. b) Clayey soil

 c) Clayey loam soil d) Silt loam soil

 2 The ideal pHof soil for growing bottle gourdis?

 a) 4-5 b) 8-9

 c) 3-4 d) 6-7

 3 What is the desirable soil temperature for good bottle gourd growth?

 a) 35-40°C b) 10-15°C

 c) 18°-22°C d) 40-45°C

1 Brassica oleracea L. var. botrytis is the botanical name of

 a) Cabbage c) Cauliflower

 b) Knol-Khol d) None of these

 2 Cauliflower was introduced in India in 1822 by ________ from Garden, London.

 a) Dr. Jemson b) Dr. Kalloo

 c) Herbalist Dodens d) None of these

9.1 Introduction

Water is essential for all life and used in many different ways. It is an important part of the ecosystem in which the reproduction of the bio diversity also depends. Fresh water scarcity for agriculture, industry and household purpose is not only limited to the arid climatic regions, but also in areas having high rainfall. Water scarcity is caused by low water storage capacity, low infiltration rate, high evaporation demand and larger inter annual and annual fluctuations of precipitation.

8.1 Introduction

Conservation agriculture (CA) is an approach to regenerative agriculture and land management practices based on three interlinked principles: (1) continuous minimum mechanical soil disturbance using no-tillage or reduced tillage based crop cultivation, (2) maintenance of permanent soil cover using crop residues and cover crops and (3) diversification of cropping system using economically, environmentally and socially adapted crop rotation including legume crops and cover crops coupled with other complementary agronomic management practices (FAO, 2014).

7.1 What is Soil Moisture Conservation Technique?

In situ soil moisture conservation means capturing rain water and retaining it in soil for utilization by plants for better growth and increase in biomass and grain yield. This is achieved through rain water harvesting on farm or field where crops are planted to get benefit from the conserved rainwater. It reduces rainwater loss through runoff and increases rain water infiltration time, thereby increasing the amount of rain water in the soil that will be useful for crop production.

6.1 Introduction

The birth of modern conventional agriculture coincides with industrial revolution. The identification of N, P and K as critical factors in plant growth led to the manufacture of synthetic fertilizers. While chemical fertilizers and pesticides have existed since the 19th century, their use grew significantly in the early 20 century with the invention of Haber-Bosch method for synthesizing ammonia. The rapid farm mechanization, especially in the form of tractor and combine harvester coupled with science-driven innovative management methods and resources led to the efficiency of enabling outputs of high quality produce per unit area and time.

5.1 Introduction

Indian agriculture is now at crossroads. We have come a long way. Over the past five decades our strategies, policies and actions were guided by goals of ‘self sufficiency’ in foodgrain production with main focus on rice and wheat. Indian agriculture has been successful in achieving higher production of food grains to secure food security of the nation. While, the success for increasing food grain production has been achieved without major jump in the production and productivity of pulses in the country, this was accompanied by widespread problems of resource degradation, which now poses serious challenges to meet the demands of an increasing population and lifting our people above the poverty line.

4.1 Introduction

Wheat (Triticum aestivum L.) is a major food security crop in South Asia, with 42% of the wheat in this region grown following rice (Oryza sativa L.) on 13.5 million hectares of land. Resource-conserving technologies offer considerable potential for improving the sustainability and productivity of wheat in the rice-wheat system of South Asia. These include raising input use efficiency, cutting costs, providing various environmental benefits, and ultimately improving farmer livelihoods and helping to reduce poverty.

3.1 Introduction

Rice is the main staple food for most of the world’s population and plays a key role to maintain food security of many countries. More than 90% of the rice production globally comes from Asia and also more than 90% of rice consumption is done in Asia (IRRI, 1997). In Asia, more than two billion people get almost 60-70 percent of their daily energy requirement from rice and its products (Yogeswari and Porpavai, 2018). As for India, since independence the country has witnessed a remarkable progress in rice production.

2.1 Introduction

Present day agriculture is facing more challenges like negative impacts of climate change, depletion of natural resources, increase of input costs etc. The major causes for steady decline in agricultural sustainability include intensive cultivation with conventional tillage practices, reduction of soil organic matter, soil loss by water and wind erosion, reduction in infiltration rate, formation of soil crust and continuous practice of mono-cropping. Over the years, soil and water resources are continuously overexploited leading to reduction in use efficiency of irrigation and energy.

1.1 Introduction

Broadcasting seed and harvesting meagre yields was a common method of ancient agriculture. High use of developed crop varieties, irrigation, chemical fertilizers, pesticides and farm mechanization is the main thrust of modern agriculture. Therefore, modern agriculture is an energy intensive farming system (Khan et al., 2010). Though modern technology based agriculture is achieving success to meet food demand of increasing global population, yet it creates many environmental issues such as loss of soil fertility, salinization and water scarcity.

Survival strategies of some plants help them to thrive in any adverse environmental conditions. We classify them as weeds or ruderals. Such weedy species have developed unique and effective physiological traits to withstand various stresses, and unique mechanisms to regulate growth under adverse environments. Climate change is a harsh reality observed in different parts of the world which brings new challenges in crop production and weed management.

Introduction of biotechnological tools in weed science and management has given new dimensions to weed research.

Indian tea has a global reach being the second largest tea producer in the world. Indian tea is one of the finest in the world. Darjeeling tea is the first product to get a geographical indicator tag in India. 83 % of the tea produced in India is from Assam and West Bengal and 17% from the south Indian states of Kerala, Karnataka and Tamilnadu. Weeds are a major problem in tea gardens.

Weed flora

Drymaria cordata, Bidens pilosa,, Cynodon dactylon, Panicum repens, Celosia argentea, Ageratum conyzoides, Centella asiatica, Borreria hispida, Oxalis latifolia, Mikania micrantha, Paspalam conjugatum,

Rice is the primary food source for half of the Asian population. Nearly 2,000 million in this region obtain 60 to 70 per cent of their calories from rice and its products. Considering the importance of the crop, United Nations General Assembly declared 2004 as the “International Year of Rice” (IYR). In India, the crop covers one-fourth of the total cropped area. About half of the Indian population use rice as their staple food.

Rice based cropping systems are not only important for food security but also for maintaing an ecological balance. Low land rice fields conserve water and prevent runoff .They preserve the sustainability of the ecosystem.

Parasitic weeds are a major constraint in tropical agriculture. These are plants that are adapted to live on other plants sharing their nutrients and water. They constitute 1% of flowering plants, approximately 3000 - 5000 species coming under 20 families. Parasitic weeds from five families viz. Orobanchaceae, Convolvulaceae, Santalaceae, Scrophulariaceae and Loranthaceae are considered troublesome weeds as they infest economically important crops in many parts of the world and are very difficult to control by normal weed management measures.

A major portion of the earth’s surface is water. Apart from the oceans, major water bodies on the earth’s surface include rivers, streams, lakes, ponds and canals. Plants adapted to these ecosystems form the aquatic species. When they pose a problem to navigation, fisheries and agriculture and also cause health hazards, they are categorised as aquatic weeds. Problems due to aquatic weeds has been reported from different parts of the world. They are common in warm waters. In Florida, it is reported that water hyacinth (Eichhornia crassipes) covers nearly 40,000 hectares of water resources.

When a herbicide is applied to the soil, it is subjected to various interactions with soil and surrounding environment which ultimately determines its persistence. Dissipation of these compounds from soil system is by three main processes viz., physical (volatilization, leaching and runoff), chemical (photochemical decomposition, adsorption by organic and inorganic colloids and chemical decomposition) and biological (microbial decomposition and plant uptake) 

1. Physical Processes

a) Volatilisation

Major advantage of chemical herbicides over other methods of weed control is its ease of application, which saves the cost of labour for the farmer. Effectiveness of herbicide depends on how long it remains in active form in the plant /soil. The form in which any herbicide is available in the market is known as herbicide formulation. The technical active ingredient (ai.) of the herbicide is converted to a formulations so as to improve easiness of handling, retention on leaf surface, absorption and movement in the plant. Herbicides are available in different formulations.

In a cropping systems there may be simultaneous or sequential application of herbicides, insecticides, fungicides, fertilizers, PGR during a single cropping season. The different chemicals will undergo physical and chemical changes as they interact with plant, soil and other chemical substances already applied to the plant or soil. The interaction effect of such chemical substances will be visible only at the later end of the cropping season. The responses observed in the plants can be.

Herbicides have received wide acceptance from farmers mainly due to ease of application and effectiveness. After application, herbicide molecules should penetrate plant tissue and get translocated to the target site. Once they reach the target protein they bind to them and disturb metabolic processes in plants by disrupting biosynthetic pathways and damaging cell structures by generating cytotoxic molecules that ultimately kill the plants. In this, the first step is penetration of herbicide into the plant system.

Effectiveness of spray mixtures can be increased by addition of adjuvants. Weed Science Society of America (WSSA1994) have defined adjuvant as a material in a herbicide formulation or added to the spray tank to modify herbicidal activity or application characteristics. Adjuvants enhance activity of a herbicide in different ways viz., wetting, spreading, deposit building, emulsifying or deflocculating the active ingredient. In many cases cost of adjuvant will be less than that of active ingredient. In such cases it helps to reduce the cost of the final product.

A large number of herbicides are currently available in the market hence selection of suitable herbicide needs judicious understanding of the product and the requirement. When large number of chemical options are available it can make the process of selection a bit confusing. So it is important to know the crops in which a herbicide can be used, the weeds it will control, the appropriate rate, adjuvants included in the formulation. The different uses of the chemical and the different situations for which it will be useful. In addition to these it is important to understand the mode of action of the herbicide and also the mechanism of action of the chemical.

Herbicides are chemical substances that are capable of either killing or injuring plants and so used for elimination of unwanted plants. In Latin, the term ‘herba' means plant and ‘caedere’ means to kill. There are many reasons for the increased use of chemicals for weed control. Shortage and high cost of agricultural labour prevent economic control of weeds by manual and mechanical means. Benefits offered by herbicides include efficient weed control, higher crop yield, better soil, water and energy conservation and higher income.

Deliberate use of natural enemies to control invasive pest in an ecosystem is known as biocontrol. To control invasive weed species, selective pathogens and weed seed predators are employed to reduce the population of the weed to non competitive numbers rather than total eradication of the weed species (Appleby, 2005) These can be insects, herbivorous fish, other animals, disease causing organisms or even competitive plants. This method is comparatively safer to the ecosystem, is cheaper and has long lasting impact. Difficulty in f inding host specific bio-agent with minimum adverse effect on other crop and the environment is a challenge.

Weeds have been a problem to agriculture ever sine man started cultivation. Weed species by and large invade cropped areas and account for large economic losses to the farmer. Weeds are economically more important than insects, fungi or other pest organisms (Savary et al. 2000, 2006). Globally, weeds cause the highest potential loss to crop production (34%), with animal pests and pathogens being less important (losses of18% and 16% respectively). The loss in production due to weeds has been estimated as 5% in developed countries. It is around 10% in developing countries and 25% in least developed countries. (Oerke, 2006)

Weeds have specific characters which helps them to adapt to any stressful situation and successfully, compete with the crop. They have survival strategies which help them to overcome vagaries of nature like extreme cold, heat, drought, biotic stresses and soil abnormalities. Their most important characteristic is the ability to succeed in any adverse situation even in disturbed soils. The age old war between man and weeds makes it pertinent to understand the characteristics of weeds which make them better competitors in the field.

Weeds are diverse plant species of the ecosystem which can survive adverse condition and reproduce quickly. The global compendium of plants has listed 28000 species as weeds. According to Holm et al (1977) about 8000 weeds species have been reported from agricultural fields in 124 countries and of these, 250 species are considered to be problematic. It is also to be noted that weeds from 12 families constitute 68% of the world’s worst weeds. Within these 12 families, weeds from poaceae, asteraceae and cyperaceae constitute 44% of the world’s worst weeds.

India is basically an agrarian economy where 57.8% of rural households are engaged in agriculture (Situation Assessment Survey of Agricultural Household, 2021). 48.9 per cent of the Indian work force is employed in the agricultural sector, (ILO, 2016). The estimated Indian population for the year 2050 is 1.7 billion which will raise our food demand to ∼400 million tonnes (Rao et al., 2018). In this context, Indian agricultural production system will have to take up the challenge to feed l7.5% of the global population with only 2.4% of land and 4% of the available water resources at its disposal.

• Epidemiological definitions

 • List of tests for international trade

 • OIE listed diseases

 • Solutions and reagents

 • Age and body weight of animals

 • Rectal temperature of animals

 • Normal values of blood vascular system

 • Vaccination schedule

• Nutritional diseases

 • Viral diseases

 • Prion, Mycoplasmal, Rickettsial, Chlamydial, Spirochaetal and Bacterial

diseases

 • Fungal diseases

 • Parasitic diseases

 • Toxic conditions

 • Miscellaneous conditions

• General considerations

 • Purpose

 • Scope

 • Five pillars of GLP

 • Good laboratory practice principles

• Test facility organization and personnel

 • Quality assurance programme

 • Facilities

 • Apparatus, materials and reagents

 • Test systems

 • Test and reference items

 • Standard operating procedures

 • Performance of the study

• General considerations

 • Purpose and goals of laboratory

 • Standards, guides and references

 • Accreditation for competence

 • Identification of scope

 • Test methods

 • Benefits of ISO17025 accreditation

 • Processing of application

• General considerations

 • Quality management principles

 • Objectives of 9001:2008

 1. Scope

 2. Normative reference

 3. Terms and definitions

 • Main clauses and sections of ISO 9001:2008

 4. Quality management system

 5. Management responsibility

 6. Resource management

 7. Product realization

 8. Measurement, analysis and Improvement

• General considerations

 • Biosafety levels-1 and -2

 • Biosafety level-3

 • Biosafety level-4

General Considerations

Biosafety is a combination of laboratory practices, techniques, equipments and other facilities appropriate for the protection of hazards posed by infectious agents handled in the laboratory. There are guidelines for maintaining the biosafety in laboratory at various levels described as under:

• General considerations

 • Ethics for veterinary diagnostic laboratory personnel

 • Safety measures in the laboratory

 • Accidents

 • First aid

 • Acid burns

 • Alkali burns

 • Poisoning

 • Burns caused by heat

 • Injuries caused by broken glass

 • Contamination by infectious materials

 • Bodily damage by electric shock

 • Precautionary measures

• General considerations

 • Steps in validation

 • Step1- Feasibility studies

 • Step 2- Assay development and standardization

 • Step 3- Determining assay performance characteristics

 • Step 4- Monitoring validity of assay performance

 • Step 5- Maintenance and enhancement of validation criteria

 • Measurement of uncertainity of a diagnostic test

• General considerations

 • Tuberculosis

 • Johne’s disease

 • Brucellosis

 • Infectious bovine rhinotracheitis

 • Campylobacteriosis

 • Tichomonosis

• General considerations

 • First hand information and constitution of a team

 • Gathering the disease information from records and the owner

 • Animal data

 • Previous illness

 • Present disease

 • Proforma for animal examination report

 • Observation of team

• Logical clinical examination of animals

 • Disease investigation kit

• General considerations

 • Polyacrylamide gel electrophoresis (PAGE)

 • SDS-polyacrylamide gel electrophoresis (SDS PAGE)

• Nucleic acid hybridization

 • Radio labelled probes

 • Biotin labelled probes

 • DNA finger printing

 • Polymerase chain reaction (PCR)

 • Reverse transcriptase polymerase chain reaction (RT PCR)

• General considerations

 • Cyanide poisoning

 • Nitrate poisoning

 • Nitrite poisoning

 • Oxalate poisoning

 • Strychnine poisoning

 • Arsenic poisoning

 • Lead poisoning

 • Thalium poisoning

 • Ergot poisoning

 • Carbon monoxide poisoning

 • Phenothiazine poisoning

 • Pesticide poisoning

• Allergic Tests

 • Single intradermal test

 • Tuberculin test

 • Johnin test

 • Double intradermal test

 • Comparative test

• Mallein test

 • Immunofluorescence Techniques

 • Direct method

• Indirect method

 • Immunoperoxidase Techniques

 • Detection of antigen

• General considerations

 • Agglutination

 • Slide agglutination test

 • Tube agglutination test

 • Capillary tube agglutination test

 • Plate agglutination test

 • Hemagglutination test

 • Hemagglutination-inhibition test

 • Coagglutination test

• General considerations

 • Scope

 • Microscopic examination techniques

 • Smear

 • Vital staining

• Routine histopathological techniques of sectioning

 • Collection of tissues

 • Fixation

 • Washing

 • Dehydration

 • Clearing

 • Impregnation

 • Casting of blocks

 • Trimming

 • Section cutting

• General Consideration

 • Post-mortem examination of large animals

 • Post-mortem examination of poultry

 • Steps in post-mortem examination

 • Writing of post-mortem report

 • Collection, preservation and dispatch of specimens for laboratory

diagnosis

• General considerations

 • Scope

 • Exfoliative cytopathology

 • Interventional cytopathology (Biopsy)

 • FNAC

 • Imprint cytopathology

 • Crush smear cytopathology

• General considerations

 • Direct examination

 • Culture methods

 • Some important tests used in identification of bacteria

 • Characteristics of some organisms

 • Gram positive cocci

 • Gram positive rods

 • Gram negative rods

 • Mycobacteria

 • Leptospira/spirochete

 • Rickettsia

 • Chlamydia

 • Mycoplasma

 • Fungi

 • Virus

 • Antibacterial sensitivity testing

• General considerations

 • Volume

 • Colour

 • Reaction

 • Concentration of spermatozoa

 • Motility

 • Live and dead count

 • Microbiological examination

• General considerations

 • Indications

 • Collection of cerebrospinal fluid

 • Sub-occipital puncture

 • Lumbar puncture

 • Examination

 • Physical examination

 • Chemical examination

 • Cytological examination

 • Microbiological examination