eChapters

Labour economics : The aspects of economics concerned with the supply and demand for labor. This includes factors affecting the participation rate, wage bargaining and organized labor, training, hours and conditions of work, practices concerning hiring, redundancy, labor turnover, migration and the age of retirement.

Labour intensive : A production process that involves comparatively large amounts of labour; the opposite of capital intensive.

Labour market flexibility : A flexible labour market is one in which it is easy and inexpensive for firms to vary the amount of labour they use, including by changing the hours worked by each employee and by changing the number of employees. This often means minimal regulation of the terms of employment (no minimum wage, say) and weak (or no) trade unions. Such flexibility is characterised by its opponents as giving firms all the power, allowing them to fire employees at a moment’s notice and leaving workers feeling insecure.

Labour theory of value : The notion that the value of any good or service depends on how much labour it uses up. First suggested by adam smith, it took a central place in the philosophy of karl marx. Some neo-classical economists disagreed with this theory, arguing that the price of something was independent of how much labour went into producing it and was instead determined solely by supply and demand.

NAIRU : The non-accelerating-inflation rate of unemployment (see natural rate rate of natureal rate of unemployment).

Nash equilibrium : An important concept in game theory, a Nash equilibrium occurs when each player is pursuing their best possible strategy in the full knowledge of the strategies of all other players. Once a Nash equilibrium is reached, nobody has any incentive to change their strategy. It is named after John Nash, a mathematician and Nobel prize-winning economist.

Nation building : Creating a country that works out of one that does not - because the old order has collapsed (as in the former Soviet Union), or been destroyed by war (Iraq), or never really functioned in the first place (Afghanistan). To transform a failed country can involve establishing order through the rule of law and creating legitimate government and other effective social institutions, as well as a credible currency and a functioning market economy. Nation building is rarely easy, and often fiendishly difficult, especially where there are deep ethnic, religious or political divisions in the population or the country has no history of ever functioning effectively. Outside expertise, such as from the world bank, and money (as in, most famouly, the marshall plan) can help, but they are no guarantee of success.

Adult female is called bitch and adult male is called dog.

Female comes to heat which is called season. Comes to season for first time at 6-8 months of age. Smaller breeds mature faster than large breeds.

SYMPTOMS OF HEAT

Females in heat becomes nuisance to both owner and public since the animal becomes unmanageable, goes out of house and sometimes street male dogs gets into house because of peculiar pheromone smell.

Has different stages

Okra

Genetic Resources

Okra [Abelmoschus esculentus (L) Monch.] s a crop of the tropical and subtropical owland regions of Africa, Asia, America and warmer temperate region of the Mediterranean. It is believed to be originated from tropical Asia or may have been existing in Africa and India as polyphylatic species. A. tuberculatus, one of the ancestors of okra occuring in India, has nine wild taxa, of which A. esculentus is popularly grown for pod production. The main region of cultivation and diversity of okra in India, Sri Lanka, Pakistan, Nepal and Bangladesh, are represented by various local cultivars of A. esculentus and A. moschatus (Table1).

1. Mitochondria

Mitochondria are known as the “Power House” of a cell because they are the site of ATP production. In addition, they are also involved with photorespiration and oxidation of fatty acids and work in association with peroxisomes, glyoxysomes and chloroplasts.

Isolation of intact mitochondria is rather difficult. This is because the cell walls of plant tissue are rigid as compared to the single-layered wall surrounding the mitochondria. A significant fraction of mitochondria suffer structural damage during the process of tissue homogenization. Therefore, the pelleted mitochondria need further purification by sucrose gradient centrifugation.

Mitochondria can be isolated from green as well as etiolated leaf; however, the latter is preferred to obtain higher yield of mitochondria (Moore and Proudlove, 1983).

Microorganisms live as single cells in nature in association with other cells in assemblage that we call “Population”. Such populations are composed of group of related cells generally derived by successive cell divisions from a single parent cell. The location in an environment where a population generally lives in association with other populations of cells called “Microbial communities”. Environmental microbiology deals with the study of these microbial communities in different habitats, naturally occurring on earth.

Natural habitats of microorganisms are exceedingly diverse. Any habitat that is suitable for the growth of microorganisms can also support the growth of other microorganisms. But in addition, there are many habitats where, extreme environment, due to chemical and physical factors, higher organisms cannot grow but microorganisms can grow and flourish. In this chapter, different habitats of microorganisms are discussed briefly.

Because microorganisms are so small, their habitats are also small. The term microenvironment is often used in ecology to describe where a microorganism actually lives and metabolites within its habitat.

Nutrient

Definition: Those elements are required for plant metabolism in more or less quantity, is known as nutrients. Plants have 30 types of elements but 17 elements out of 30 are essential for plant growth and development. These elements are called Essential nutrients.

Criteria of essentiality of plant nutrients theory: It is given by Arnon and Stout (1939). This theory stated that plant can not be completed their life cycle without the deficiency or complete absence of a single nutrient among the essential nutrients

Low of minimum: It is given by Justus Von Leibing (1840). According to this low, the growth of the plants is limited by the plant ntruient present in smaller quantity, all other being in adequate amounts. This has been re–stated as Barrel concept.

Allen and Arnon (1955) laid out following criteria for categorising nutrients essentiality to plants:

1. Complete or partial lack of the element in question must make normal plant growth impossible
2. Deficiency symptoms must be reversibly by the addition of the elements in question
3. The element must play specific role in the plant metabolic symptom

Soil fertility

• Soil serves as a source of nutrient required for the growth and development of plant. This characteristic of soil is called soil fertility.

Management practices

• Improved organic matter and soil moisture in soil, favours the growth of micro-organism beneficial for maintaining fertility of the soil.

Manures

• It is an organic waste material of plant and animal origin. It is used improvement of soil fertility. There are major 2 types of manure

1. Bulky organic manures
2. Concentrated organic manures

• Botanical name: Papavera somnifera
• Family: Papaveraceae
• Origin: Europe
• Useful part: Fruit/Capsule
• Chemical content: Morphine, Narcotine, Codine
• Flowering time: 95–115 days after sowing
• Capsule maturity: 15–20 days after flowering
• Uses: It is also called “God of sleep”. It is used in Painkillar, hypotonic effect and cure to leukaemia
• It is a licence crop and grow in Aari
• One aari equals to 100 m²
• Seed rate: For broadcasting: 7–8 Kg/ha

  For line sowing: 4–5 Kg/ha

• Thinning is important cultural practices.

Abstract

The seaquake on 26^th December 2004 with its epicenter at Sumatra triggered a tsunami, which had a major impact on South East Asian countries. During that incident, the average height of the tsunami wave was 7-10 m and the penetration of seawater was up to 1-1.5 km. The surface water resources meant for irrigation and other drinking purposes got affected by the ingress of seawater in many areas. The direct environmental impacts of the tsunami have more impact along the entire east coast and caused more damages in coastal systems. The physical and chemical perturbations caused in the coastal zone by the tidal waves are discussed. In order to assess the environmental impact on coastal system, it is suggested that significant physical and biogeochemical factors could be framed to quantify the problems.

Keywords: Tsunami, Physical and Chemical Alterations, Coastal zone.

5.1 The Need

In spite of the considerable experience and quite a few facilities available in the country, the growth of application of isotope techniques is not commensurate with India’s size, its climatic and hydrogeological diversity and the sheer range of its water resources problems.

The situation may not be unique to India and other developing countries. Australia also appears to be facing a similar problem. A recent publication [1] says ‘the limited uptake of the method may be due to a number of factors. Until relatively recently, isotope hydrology has remained a largely academic pursuit, or measurements were the domain of national atomic energy agencies. A large fraction of information lies buried in the grey literature or in arcane technical journals. Interaction with local or regional water resources agencies has been the exception rather than the rule’

India has probably a stronger case for a more effective use of isotope techniques. A large portion of groundwater occurs in hard rocks in India. Normal hydrogeological and geophysical methods cannot fully describe such groundwater regimes to effectively plan their exploitation. It is essential to apply tracer hydrology methods, especially isotope geochemical techniques, for a comprehensive understanding of the systems. The examples of Manikaran geothermal springs, seepage studies in Salal tunnel and in some dams, given in the earlier chapters come under this category. Studies on percolation tanks in basaltic terrain and on recharge processes need isotope support.

It will not be long before we would be planning to exploit very deep ground waters (>500 m), both in the hard rock terrains of peninsular India as well as in the Ganga plains to meet our ever growing need for water. We would need good understanding of the recharge processes to these deep ground waters. Isotopes will have a large role in such investigations.

Arid zone hydrology is yet another area where the potential of isotope techniques has been effectively demonstrated for the characterization of the recharge processes, both palaeo and modern, and including the role of ancient rivers.

Abstract

The analysis of IRS - 1A satellite imagery has indicated the existence of two NE - SW trending sub parallel faults along which the coast is undergoing land subsidence as revealed by the concavity in the shore line, preferential migration of the river systems and the drainage congestion, absence of beach ridges, occurrence of off shore shoal etc. The study has further indicated chains of tectonically induced environmental problems such as ground water logging, coastal zone flooding, storm surges, harbour sedimentation etc.

5.1 Impact of climate change on agriculture

Climate change has both positive and negatively impact on agriculture, food security and land degradation. Climate change could exacerbate the land degradation processes by increases in rainfall intensity, drought frequency and severity, dry spells, f looding, heat stress, sea-level rise and permafrost thaw. The 1/4^th of ice-free land area in the world is prone to human-induced anthropogenic degradation (Certini and Scalenghe, 2011; Hooke et al., 2012). Soil erosion rate is 20-100 times faster than that of soil formation in agricultural field. The erosion rate is least is zero-tillage (non-disturbed field) and maximum at conventional intensive tillage practices. Climate change further exacerbates the land degradation. The permafrost areas, dry-lands, low-lying coastal regions, river deltas are more vulnerable. The occurrence of drought in dry-land was increased by around 1% per year during 1961-2013 periods. On an average 380-620 million people globally experienced desertification who lived in dry-land areas (1980s and 2000s) in South and East Asia, North Africa, Middle East, Sahara region, and Arabian peninsula. Moreover, people who are already living in degraded/ desertified regions are more negatively affected by climate change.

Specifically, the land surface air temperature has increased about twice as much as the global average temperature since the pre-industrial era. The land-surface air temperature has increased relatively higher than the world’s average surface (land and ocean) temperature since the pre-industrial era (1850-1900). The average land-surface air temperature has enhanced by 1.53°C (1.38-1.68°C), whereas, world’s surface (including land and ocean) air temperature has enhanced by 0.87°C (0.75-0.99°C). Subsequently, global warming triggered the frequency, intensity and duration of heat waves and related damage. The direct and indirect impacts of climate change on global agriculture is summarised below for an overall idea about the importance of the issues.

Coastal ecosystems of india

India has a long coastline of 7,517 km that span 13 maritime states and union territories, surrounded by the Indian Ocean, Arabian Sea and Bay of Bengal. It has an Exclusive Economic Zone of 2.02 million square kilometers. The country has a diverse range of habitats: continental area and offshore islands and a variety of coastal ecosystems such as estuaries, lagoons, coral reefs, mangroves, salt marshes, sea grass beds, backwaters, rocky coasts, mudf lats, sand dunes, and coastal beaches. Coasts and islands form 2.8% of India’s geographical area. The coastal ecosystem consists of 43,230 km² of coastal wetlands with 97 major estuaries and 34 major lagoons; 4921 km² of mangroves with 31 mangrove areas, and 3062.97 km² of coral reefs with 5 coral reef areas (MoEFCC, 2018). A network of 14 major, 44 medium and numerous minor rivers together with their tributaries with a total length of over 40,000 km, contribute to the coastal sea directly or indirectly. By area, tidal/mudf lats are predominant and by length, more than half of the Indian coastline is sandy. The states of Gujarat, Tamil Nadu and Andhra Pradesh have extensive coastline (Fig. 1).

The aggregate of data forming a subject of investigation is known as population. Example, All the crop plants grown in a plot; all the fruit plants in an orchard, etc. The population may be:

1. Finite: No. of observations are definite. e.g., number of farms in a village, no. of house in a locality, no. of trees in an orchard are all finite population.
2. Infinite: Number of observation are indefinite e.g., (a) Number of insects in a region, number of grasses in a field etc. are all infinite population.

The Population may be:

1. hypothetical or theoretical

   Hypothetical population consists of unit that has no physical existence. This population exists only in imagination. For example, Result of tossing coin, throwing die,

2. Existent or real population consists of units that are physically present. This population actually exists. For example, Number of employees in a factory, Number of farms in a village.

INTRODUCTION

Mass production was the hallmark for improvement of productivity in early 20^th century. Subsequently the productivity development was driven by the use of information technology and related concepts like Entrepreise resource planning (ERP), Customer relationship Management (CRM), Business Process Reingineering, TQM etc. These technologies and concepts were instrumental in collection, codification, analysis, decision making and execution of decision which translated to better coordination and profitability. The ability to harness synergy out of the information systems, coordinated team and goal orientation helped in executing strategies with sharpness, proactively identifying customer need, responding to social context and competitive scenario with alacrity. As it stands now, with internet and multimedia technologies it is faster to form organization, reach out to the stakeholders. Technology mediation has improved the productivity and quality of economic activities. It has still lot more options to come in the 21^st century.

Sovereign state performances from different parts of the world have been going through a different set of social and performance context. Over last couple of decades multiple social issues have surfaced. Issues like “rich and poor divide”, “increase of least developed countries (LDCs)”, have forced a thinking about the efficacy and efficiency of governance processes. The social and economic context in multiple countries gave rise to the Millenium Development Goals (MDG) of the UN. Governments in most of the countries have short life span than before, and are now elected with lower margins. The recent Jasmine revolution has eliminated entrenched dictators, almost without bloodshed vastly due to the use of technology like, social-e-media platforms. Regional aspirations have become strong, vociferous and demanding. Splintering of USSR, Sudan, Unification of Germany, Iraq, overthrowing of monarchy in Nepal and closer home in India, there is clamour for more states. In a multi-party democracy, few national parties have got splintered into or have given rise to multiple regional political parties to represent the local aspirations. Governments are now grappling with economic crisis. Democracy as the form of the government, ironically, has not been able to live upto the expectations. On different facet, recently the issues like wikieleaks and exposure of snooping of different governments on its own citizens, have indicated the lack of trust of people.

Introduction

In India, almost 85 % of our population resides in rural areas and the same proportion is dependent on agriculture for sustenance and animal husbandry for additional income. Distribution of livestock is more equitable compared to that of land and about 85 percent of livestock are owned by the landless, marginal and small landholding families. This sector provides employment for the farmers through livestock farming as well as in processing, value addition and marketing of livestock products.

Rapid population, economic growth, increased demand for livestock products such as meat and dairy products, in?uences the present livestock production systemtowards intensive production. There is increased con?ict over scarce resources such as land, water, food grains etc. Losing livestock assets for rural communities might lead to abject poverty with serious effect ontheir livelihoods. The climate change impact will worsen the vulnerability of livestock systems and those farmers depending on livestock, particularly the poor.

Vegetable crops, known as protective foods, are important for the healthy diet providing vitamins, minerals, fibre, amino acids and other bioactive compounds. Improvement in vegetable crops is inevitable for better yield, pest or disease resistance, morphological characters, nutritive quality, to adapt with environmental stress, climate change, offseason production, satisfying varying consumer preference etc. Vegetable crops comprise of a large number of species and are rich in diversity. Diverse genetic resources are pre-requisite for any crop improvement programme and vegetable improvement is carried out using exotic introductions, indigenous collection and hybrids/varieties by different breeding systems. Characterisation of genotypes is the description of germplasm giving information on heritable characters so that promising one can be utilised in breeding programme which is the best option for higher yield and other qualities. The morphological, physiological and molecular (biochemical and DNA) traits are called qualitative descriptor and yield, pest/disease resistance, stress tolerance etc are quantitative descriptors that are easily influenced by the environment factors. Apart from the higher production and resistance to biotic and abiotic factors, nutritional traits, flavour, processing qualities that increase the food value is also important in improvement programme of fruits as well as vegetables.

Genetic resources of vegetables available at international and national levels are characterised, evaluated and conserved. With the high diversity of vegetable crops, characterisation and evaluation of genetic resources become a stupendous responsibility requiring extensive infrastructure facilities and varying methods of evaluation (Srivastva, 2012). Genetic resources are characterised on highly heritable traits expressed across the environment. Apart from morphological characterisation, biochemical and molecular characterisation is also conducted that are useful for the identification of biotic and abiotic stress parameters, nutritional and processing quality. Genotype characterisation can be done efficiently using potential of biochemical and molecular markers that help to set up the breeding programme.

By Alkaline Lysis Method

Introduction

DNA of prokaryotic cells is relatively simple in comparison to that of eukaryotic cells. In addition to chromosomal DNA, a bacterium may also carry an additional piece of DNA called a plasmid. Bacterial plasmids are double stranded closed circular DNA molecules that range in size from 1 kb to more than 200kb. Plasmids are useful to bacterial cells since they carry genes for antibody resistance, RM system etc that allow bacteria to survive in non-ideal conditions.

Plasmids constructed in the laboratory are used as cloning vehicles. These synthetic plasmids contain only essential features such as origin of replication, antibiotic resistance, multiple cloning sites etc. various copy number of plasmid For e.g. pBR322 20 copy/cell – low copy number, pUC 18, pUC 18- 800 copy/cell – high copy number.

Alkaline lysis, in combination with the detergent sds, has been used for more than 20 years to isolate plasmid DNA from E.coli (Birnboim and Doly 1979). This experiment describes how to extract plasmid from bacteria – which involves growth of bacterial culture, harvesting and lysis of bacteria and isolation of plasmid DNA from the lysate using alkaline lysis method. The DNA is analysed on agarose gel.

Introduction

Ligation of DNA fragment is one prime important in genetic engineering because without ligation we could not transfer the interest gene.DNA cloning requires the DNA sequence of interest to be inserted in a vector DNA molecule. For this, both the vector as well as insert DNA is prepare by digestion with compatible restriction enzymes, so that the ends produced during digestion is complementary in both. When setting up ligation, it is important to consider the permutations that can occur and bias the relative concentration of DNA accordingly. Usually a 5- to 10-fold excess of insert over the vector DNA is the norm. This ensures that enough ligated product will be produced in the right orientation.

Introduction

Western blot analysis can detect one protein in a mixture of any number of proteins while giving you information about the size of the protein. It does not matter whether the protein has been synthesized in vivo or in vitro. This method is, however, dependent on the use of a high-quality antibody directed against a desired protein. So you must be able to produce at least a small portion of the protein from a cloned DNA fragment. You will use this antibody as a probe to detect the protein of interest.

Western blotting tells you how much protein has accumulated in cells. If you are interested in the rate of synthesis of a protein, Radio-Immune Precipitation (RIP) may be the best assay for you. Also, if a protein is degraded quickly, Western blotting won’t detect it well; you’ll need to use (RIP). See the section on RIP for more information, as well as a helpful comparative chart that illustrates the differences between these two techniques.

Soil pollution is any physical or chemical change in soil that adversely affects the health of plants and other organisms living in and on it.

Soil pollution is important not only in its own right but because so many soil pollutants tend to move into surface water, groundwater, or air.

The chief soil pollutants are salts, petroleum products, heavy metals, and agricultural chemicals. Point source releases of industrial solvents (both LNAPLs and DNAPLs) and feedstock chemicals (e.g., for making plastics) often occur as the result of LUSTs (leaking underground storage tanks).

Salinization, a common problem in irrigated arid and semiarid regions, makes soil unfit for growing most crops. It is extremely difficult to remove excess salts from salinized soils.

A variety of techniques are used for soil remediation, which is cleaning up contaminated soil.

1. These include physical techniques such as
   1. Soil vapor extraction (SVE), moving air through the soil.
   2. Thermal conduction heating, heating the soil before SVE.
2. Bioremediation methods include
   1. Landfarming, fertilizing, liming, and repeatedly plowing contaminated soil to stimulate bacterial decomposition of high molecular weight hydrocarbons like those in diesel oil.
   2. Bioventing, pumping air at low rates through soil to provided O₂.
   3. Biosparging, pumping air into aquifers to provided O₂.
   4. Phytoremediation, particularly useful for removing heavy metals.

Nose

The nose is the externally visible part of the respiratory system. It forms the part of the face of the animal extending from the transverse level of eyes to the rostral end of the head. Exteriorly it is formed by the bones, muscles, cartilages and the skin. Internally it encloses the nasal cavity which is lined by the mucous membrane. The dorsal part of the nose (roof of the nasal cavity) is termed as dorsum nasi. It is formed by the nasal and frontal bones of the skull. The lateral parts are formed by the incisive, maxillary, lacrimal and zygomatic bones. Covering these bones externally is a muscular layer formed by the facial muscles running over these bones and acting on the upper lip and nostrils. The muscular layer is externally covered by the skin. The skin on the nose bears fine hairs except at the tip of the nose that is formed by the nostrils. The rostral end of the nose is formed by nostrils which provide entrance to the nasal cavity. The nostrils are supported by cartilages which are the dorsal and ventral extensions of the cartilage of the nasal septum. The main aim of the nose is to support and protect the nasal cavity.

The skeleton of thorax (Fig. 3-1) consists of a bony cage formed by thirteen thoracic vertebrae constituting its dorsal boundary and roof, thirteen pairs of ribs forming its lateral walls, the costal cartilages which assist the ribs to form the lateral walls and the sternum forming its floor.

The general architecture of the vertebrae and the vertebral column is described in the chapter on skeletal system under PART ONE (Systemic anatomy) of this book. Only the deviations from the general characteristics of vertebrae as occur in the thoracic vertebrae are described here in the following account on the thoracic vertebrae.

Thoracic vertebrae

These are thirteen in number in ox (Fig. 3-1 A and B) The characteristic features of these vertebrae are the presence of capitular facets for articulation with the head of the rib and the greater development of dorsal spinous processes (neural spines).for attachment of muscles of the back and the supraspinous ligament that helps in the ligamentum nuchae to extend and suspend the head with the body trunk.

Surface anatomy of the organs of left side of abdominal cavity

Rumen

Topography of the rumen: The left side of the abdomen in cow is completely occupied by the rumen except for a small area occupied by the reticulum at its cranial end and the spleen on the craniodorsal aspect sandwiched between the dorsal border of rumen and the diaphragm. The rumen lies in contact with the left abdominal wall extending from seventh or eight intercostal space where it touches the diaphragm to the transverse plane of the tuber coxae where it almost touches the pelvic inlet.

Palpation of rumen: The rumen can be palpated in the left flank of the abdomen and is usually palpated in the dorsal third of the flank region. In ruminants, the contraction begins at reticulum and it continues in rumen in succession and hence is considered as ruminoreticular contraction cycle. It begins with double contraction of reticulum followed immediately by the contraction of rumen. The rumen contraction begins in the cranial sac and progresses to the dorsal sac and then to the ventral sac. During the contraction of the ventral sac, the dorsal sac is forced out which appears as a bulging at the paralumbar fossa. After this, there is a pause of 30 seconds and thereafter the second ruminal contraction begins which is usually associated with eructation. The ruminoreticular contraction cycle ends with the end of second ruminal contraction. Following this, the second cycle begins. Thus there are two palpable rumen contractions per minute. This rate of contraction increases to 2.8 contractions per minute during the act of feeding of the animal.

Objectives

• Differentiate between fixatives and stains.
• Describe methods of staining.
• List the commonly used fixatives and stains and their composition.
• Describe the uses of above mentioned solutions and stains.

Introduction

Every time a part of an organism is removed, the action of bacteria will soon disintegrate, dry or putrefy it. They must be kept as close to normal as possible or in other words preserved and fixed in order to study these. Chemicals are required in many practical of Biology. Some of these chemicals preserve and specifically colour certain cell parts while others are used as solutions. You will learn about certain fixatives (preservatives) and stains in this chapter.

Objectives

• Detection of linkage using Chi-square test for goodness of fit.

Introduction

The tendency of two or more genes to stay together during inheritance is known as linkage and it is the consequence of the concerned genes being located in the same chromosome. Linked genes do not show independent segregation, as a result the ratios obtained in F₂ and test cross generation are significantly different from the expected ratios of 9:3:3:1 and 1:1:1:1, respectively.

Detection of linkage χ² using test

In genetic experiments, when the material under study is segregating simultaneously for two characters, it may be of interest to know whether the two characters are inherited independently or they are linked. The χ² test can be used for this purpose. χ² test is used in detecting linkage in back crosses and inter crosses.

First the χ² is computed based on the theoretical segregating ratios. If χ² is not significant it may be concluded that the loci segregate independently. If χ² is significant we may suspect linkage between the loci. The χ² is then partitioned into components assignable to different factors.

Objectives

• Study of gene mapping.

Introduction

All the genes that are linked together form a linkage group. Genes of a linkage group may be presented on a single straight line in the same order in which they are normally present on the concerned chromosome. The number of different linkage groups in a species is, as a rule, equals to its gametic chromosome number (n). The distance between two neighbouring genes is proportional to the frequency of recombination (%) between them depicted on a line drawing. Such a line drawing depicting the linked genes the recombination frequencies between them is known as Linkage map, Genetic map or Chromosome map. Linkage maps provide information on the genes that are linked together and the frequencies of recombination that may be expected between them. The frequency of recombination between two linked genes cannot exceed 50% which is the frequency in case of independent segregation.

Objectives

• Better understand the phenomenon of probability.

Introduction

The concept of probability is basis to many principles of classical genetics (segregation, independent assortment etc.) The principals of population genetics are based on the considerations of laws of probability. Probability is the likelihood of occurrence of an event.

Trial

A trial refers to an opportunity provided for an event to occur, e.g. tossing of a coin once constitute one trial as it provides an opportunity for head or tail to occur. A new borne can be either a boy or a girl.

The discovery of mutagenic effects of X-rays on the fruit fly (Drosophila) by H. Muller in 1927 and by L. J. Stadler in barley and maize in 1928 paved the way for researchers to experiment with its effects on various organisms. In 1928, H. Stubbe showed the use of mutagenesis in producing mutants in crop plants like tomato, soybean and others. The first commercial mutant was produced in tobacco in 1934. Reports by B. Sigurbjornsson and A. Micke mentioned 77 cultivars that were developed through mutagenesis prior to 1995. In 1995, this number was 484. This number has since been significantly increased covering a large number of crops, namely, corn, wheat, pea, chrysanthemum, poinsettia, dahlia, citrus, apple, peach and the modified traits include plant maturity, winter hardiness, lodging resistance, protein and lysine content and numerous ornamental mutants. By the end of 20th century, the number of mutant varieties rose to 2252 covering a range of crops like cereals, oilseeds, pulses, vegetables, fruits, fibres, and ornamentals. Majority of these mutants were released as direct mutants and the rest as a result of cross breeding with the mutants. Most of the mutant varieties have come as a result of using physical mutagens (X-rays, gamma rays, thermal and fast neutrons) with gamma rays alone accounting for more than 60% of the mutant varieties. By 2017, nearly 3250 mutant varieties belonging to 175 plant species have been developed and released where China, Japan, India, Russia, the Netherlands, Germany and USA are the leading countries. India with 345 commercialized mutants stands third after China (810 mutants) and Japan (481 mutants). Thus mutation breeding is a significant contributor to the development and release of commercial cultivars in several crops and for several traits.

The concept of recurrent selection grew out of need to increase the number of superior genotypes that could be obtained from breeding stock of corn. This could have been possible by increasing the frequency of superior genes in the gene pool and by increasing the chances for genetic recombination to occur. Both can be achieved by recurrent selection which is defined as “a method of breeding designed to concentrate favourable genes scattered among a number of individuals by selecting in each generation among the progeny produced by mating inter se of the selected individuals (or their selfed progeny) of the previous generation.” The original idea behind recurrent selection was proposed first by Hayes and Gerber in 1919 and independently by East and Jones in 1920. Jenkins (1940) was the first to describe the method in detail and Hull (1945) proposed the name ‘recurrent selection’. Each cycle of recurrent selection requires (i) evaluation and selfing of selections and (ii) crossing of the progenies of superior selfed plants in all combinations and bulking of equal amount of seed from each cross. The selection of plants before selfing is possible for maturity, plant height, ear/head characters, kernel characteristics and resistance to diseases and insects. In general, these are the characters which have high heritability. It is clear that the selection of plants before selfing is more for morphological traits and these evaluations do not include yield. Yield evaluations are done on the top cross progenies (crossing of selected plants to a tester). Such top crosses will be grown in the second year of the cycle during which time the selfed seed is held over until the evaluations for yield are over. Or the selfed seed may be grown out as S₁ lines which in turn are selfed. After yield evaluation is over, the selfed seed of superior plants is grown out for crossing. Progenies of selfed plants are crossed in all possible combinations. This is done manually or by growing progenies together in an isolated block. In isolation planting, random mating should be ensured. After manual crossing or random mating in isolation, equal amounts of seed of each are bulked and this bulk seed is used to start the second cycle of selection, selfing and crossing.

When conditions in the plant growing environment are less than the optimum, the plants experience stress which is broadly termed as abiotic stress. Major abiotic stresses impacting crop yield significantly are as follows (Grover et al., 2004; Acquaah, 2007):

Drought: This is the environmental condition caused by lack of adequate rainfall. This is also termed as water stress or desiccation stress. Effects of drought on crop plants are complex and variable and are greatly accentuated by a number of interacting factors. The onset of drought in general has been observed to reduce germination, emergence, hypocotyl length, water uptake and the mobilization of dry matter reserves even at the early growth stages. Drought stress causes a marked reduction in leaf area. Soil moisture availability at the seed germination, anthesis and at post-anthesis growth stages determines as to what extent production potential of crops would be expected in drought-prone situations.

The yield losses in field crops may be attributed to inadequate water availability at the critical growth stages. Crops therefore differ in their susceptibility of growth stages to water stress. Stress exposed plants immediately lower down relative water content (RWC) of their leaves, leaf water potential, osmotic potential. Turgor pressure may also be decreased unless plants have finally adapted to drought by opening of stomata again and improving the water potential. It has been reported that low leaf expansion rate at the early stage and accelerated leaf senescence accompanied by mortality of older leaves at later stage decreases total leaf green area.

When there is indication of linkage from F or testcross data through significant deviation from 9: 3: 3: 1 and 1: 1: 1: 1 ratio, it is implied that independent assortment is not taking place and this is taken as an indication of linkage. The inverse of linkage strength is the recombination value and is often symbolized by ‘p’ whose maximum value is 50% because out of four strands of a tetrad, two are always parental types and two are recombinant types when all the cells show crossing over.

1.1 WHAT IS PLANT PROPAGATION?

Propagation of plant is defined as multiplication and perpetuation of new individuals from a selected plant having all the characters of the original one. It is the process of increasing the number of plants of a particular species or cultivar. It refers to the multiplication of an individual plant or group of plants, which have specific value to mankind. New plants or new individuals are required for establishing new plantings/new gardens/new orchards.

Precision farming involves the collection of data by observation and measurement, and the subsequent response. Sensing is key to the collection of data; whether this is remote from space by satellites, by aircraft or drones, or by proximal sensors used on the ground, mounted on vehicles or otherwise. Responses are increasingly automated and data are often integrated into wholefarm management systems. Early commercial applications were directed towards improving crop nutrition, however, utilising precision technologies to control weeds, pests and diseases is now a reality.

Precision crop protection requires detailed data on the occurrence and distribution of problems at the earliest possible stage of detection. An early detection is the key for successful management of pests in precision farming. Some of the key aspects are:

Identification: Identifications of crops, targets and beneficial; temporal and spatial distribution: presence of the target before, during and after the crop growth period;

Sampling techniques: statistically valid sampling regimes must be used to permit an accurate view of any problem

Thresholds: under particular circumstances, the point at which action to ensure levels of infestation or damage are kept to those deemed to be acceptable;

Prediction: the speed and pattern of development of a problem must be capable of being modelled, e.g. spread and severity of insect pests and fungal diseases; weed emergence according to species, dormancy and environmental conditions.

Apple is the pomaceous fruit (Malus domestica) in the rose family (Rosaceae). It is one of the most widely cultivated tree fruits, and the most widely known of the many members of genus Malus that are used by humans.

The apple originated in South-West Asia which includes Asia Minor, the Caucasus, Soviet Central Asia, Himalayan India and Pakistan. It is grown extensively in the temperate region. The major apple growing countries are Australia, Argentina, Brazil, China, Canada, Denmark, Egypt, France, Gemany, USA, India, etc. In India states of Jammu and Kashmir, Himachal Pradesh and mountanous region of Uttar Pradesh are the major apple producers.

Cultivars/Varieties: The apple varieties are divided into three groups based on their maturing periods. The early season varieties grown in the states of Himachal Pradesh, Jammu & Kashmir and Uttranchal are: Tydeman’s Early(P), Irish Peach, Early Shanburry(P), Michael Molies, Benoni Fenny, Benoni Delicious, Schlomit Starkrimson, and Chaubattia Princess. The Mid season varieties are: American Mother, Red Delicious, Richared Razakwar, Starking Delicious, Vance Delicious, Jonathan(P), McIntosh(P), Top Red Lord Lam, Cox’s Orange Pippin(P), Cortland bourne(P), Red Chief, Red Gold(P), Golden Delicious(P), Oregon Spur, Queen,s Apple, Redspur, Rome Beauty, Scarlet Siberian. The late season varieties are: Golden Delicious(P), King’s Pippin, Rymer, Yellow Newton(P), American Apirouge, Buckingham(P), Winter Banana, Kerry Pippin, Granny Smith(P), Sunhari Chamure, Golden Delicious(P), Red Delicious, Ambri Baldwin, and Yellow Newton(P).

Bael (Aegle marmelos, L.) belongs to the family Rutaceae and is an important indigenous fruit of India known since 2000-820 BC. Bael is considered to be native to India and is grown all over the country. It is also seen in south east Asian countries like Sri Lanka, Pakistan, Bangladesh, Burma, Indonesia and Thailand. It is a hardy plant and can be grown even on marginal soils.

The leaves are trifoliate and aromatic. The leaves of the tree are used in religious offerings. The fruit is usually globose, grey or yellowish with small hard aromatic rind. The pulp is thick mucilaginous, orange yellow in colour with sweet scented aroma. The importance of bael fruit lies in its curative properties. It is used in Ayurveda for its medicinal properties.

There are no recognized or improved varieties of bael with a standard name. It usually goes with the name of locality or region it is grown in. Ayodhya, Etawah, Faizabad Local, Gonda Selection, Kagzi Bael, Kagzi Kalan, Mirzapuri, Darogaji, Ojha, Rampuri Azamati, Khamaria, Deoria Large, Gonda-1 and NB-5 are a few to name.

Uses

Bael fruits cut in half, or the soft types broken open, and the pulp, dressed with palm sugar, eaten for breakfast, as is a common practice in Indonesia. The pulp is often processed as nectar or “squash” (diluted nectar). A popular drink (called “sherbet” in India) is made by beating the seeded pulp together with milk and sugar. A beverage is also made by combining bael fruit pulp with that of tamarind. These drinks are consumed perhaps less as food or refreshment than for their medicinal effects.

Mature but still unripe fruits are made into jam, with the addition of citric acid. The pulp is also converted into marmalade or sirup, likewise for both food and therapeutic use, the marmalade being eaten at breakfast by those convalescing from diarrhea and dysentery. A firm jelly is made from the pulp alone, or, better still, combined with guava to modify the astringent flavor. The pulp is also pickled. The young leaves and shoots are eaten as a vegetable in Thailand and used to season food in Indonesia. They are said to reduce the appetite. An infusion of the flowers is a cooling drink. (Morton, 1987).

Mango (Mangifera indica L.), the choicest fruit of India, is said to be the king of fruits. Mango belongs to the family Anacardiaceae and genus Mangifera. The fruits are lusciously juicy, with pleasant aroma and sweet taste. In nutritive valve and popularity, no other fruit can match it. It is an outstanding source of vitamin A, a good source of vitamin C, apart from normal minerals and other vitamins. In, India, it occupies the same position as apple does in temperate countries and grapes in certain other regions.

According to de Candolle, mango has been under cultivation in India for over 4000 years. References indicate that Alexander the Great noticed a beautiful mango plantation in the Indus Valley in 327 BC. Early foreign traveller like Huen-tsang (632-645), Ibn-Haukul, (902-968 AD) Ibn- batuta (1325-1349 AD) and Ludovici de Varthema (1503-1508) also praised the mangoes grown in India and mentioned it in their travel notes.

Mango is native to southern Asia, especially eastern India, Burma, and the Andaman Islands. Besides India, mango is cultivated as a commercial crop in many countries, such as, Pakistan, Bangladesh, Myanmar, Sri Lanka, Thailand, Vietnam, Malaysia, the Philippines, Indonesia, the Fiji Island, Tropical Australia, Egypt, Israel, Sudan, Kenya, Uganda, Tanzania, South Africa, the USA (Florida, Hawaii and Puerto Rico), Mexico, Brazil, Haiti and West Indies.

Varietie/Hybrids: Dashehari, Langra, Chausa, Fazli, Malda and Bombay Green are the popular varieties in Northern and Central India. Alphonso, Kesar, Pairi, Rajapuri, Jamadar, and Totapuri are widely grown in Western India. Bangenapalli, Suvarnarekha, Neelum, Totapuri, Malgoa and Alphonso are popularly grown in Southern India and Gulabkhas, Kishen Bhog, Himsagar, Zardal, Fazli and Langra are grown mainly in Eastern part of the country. Mallika, Amrapali, Manjeera, Ratna, Niranjan, Neeleshan, Neelishwari, Neelphonso, Arka Aruna, Arka puneet, Arka Anmol, Sidhu and CISH-M1 are some of the hybird varieties available.

The history of gardening in India is older than civilizations of Mohenjo-daro and Harappa. During the pre-Aryan period (around 2500 B.C.), Indians were cultivating flowers and plants of religious significance. Later importance of gardening was not properly understood in India. But presently, importance of landscaping and gardening is realised by planners and landscaping has now become an integral part of any new establishment or property. Due to urbanization and industrialization, pollution has increased considerably and landscaping is one of the best options to reduce the problem. Landscaping is an art of beautifying a piece of land or garden or a landscape with planting materials and non-living materials in order to create a picturesque effect or to imitate nature in that particular land for human use. The importance of landscape gardening can be divided into three categories.

An unbranched evergreen tree of tropical and warm regions with a crown of very long feathered or fan-shaped leaves and typically having old leaf scars forming a regular pattern on the trunk. Palms have great ornamental value and are planted in the garden for special effects.

Palms are native of tropics and belong to Palmae or Arecaceae family. They are varied in forms, size and habit of growth. Palms do not increase in thickness and also do not show any distinction in bark wood and pith. Flowers are produced on spikes that hanged gracefully in many species. Inflorescences are highly diverse from large panicles with many orders of branching to simply spikes. Flowers may be bisexual or unisexual borne either on the same plant or on separate plant. Whereas, some palms are polygamous.

Introduction

Bird of Paradise is known as Strelitzia as its genus is Strelitzia. The shape of flower and its brilliant orange and blue colour it resembles bird and called bird of paradise. Due to unique appearance of the flower, it is exceptionally popular as a cut flower which appears periodically throughout the year. Commercial production of cut bloom is done in California, Hawaii, Israel, France, South Africa and Japan.

Bird of paradise occupies a place of pride in the garden and is an important choice for landscaping. It is grown in backyard and also for mass effect. Plants of strelitzia can be used in the landscape in a low growing ground cover to enhance the beauty of garden. They serve well anchoring island beds when surrounded by lower growing annuals or ground cover.

Introduction

Crossandra is an important traditional flower crop of South India. The word crossandra is derived from Greek words ‘krossoi’ meaning fringe and ‘aner’ meaning male, thus word crossandra means fringed stamens. It is grown for loose flower production and commonly used in temple offering and for making Gajras and Venis to use as hair adornments. Owing to their attractive colours, light weight and good keeping quality, crossandra flowers are ideal for garland making. Steady market demand and assured and regular income have made crossandra a profitable venture for South Indian farmers.

Genetics

The genus Crossandra belongs to family Acanthaceae. The diploid chromosome number of crossandra is 2n=40. This genus consists of around 50 species of tropical plants with only a few cultivated species such as Crossandra undulaefolia, C. mucronata, C. guineensis and C. sebacaulis. Crossandra undulaefolia is an important species which is grown for commercial value across the world.

Introduction

The WTO was established on 1^st January 1995 and is responsible for making and enforcing rules for trade between nations. WTO marks a major change in global trade rules. As an organization, it replaces the General Agreement on Tariffs and Trades (GATT), which had been in existence since 1947. The Eighth Round of Multilateral Trade Negotiations under GATT, which started in Uruguay in 1986, was concluded in 1994, leading to the creation of WTO as the new permanent international trade organization. The role of WTO is much more extensive than that of GATT, which dealt with trade in goods. Apart from goods, the two other broad areas that WTO covers are services and intellectual property, which previously belonged to the domestic domain. Accordingly, WTO administers not only the Multilateral Trade Agreements (MTAs) in goods but also the General Agreement on Trade in Services (GATS) and the Agreement on Trade Related Aspects of Intellectual Property Rights (TRIPS), which came into existence with WTO. All the agreements annexed to the Agreement establishing the WTO were signed as part of a package deal. Member countries did not have the option of choosing some and rejecting others. Another important difference with the erstwhile GATT is that WTO has a stronger compliance mechanism (Chawla, 2007a).

As one of the WTO agreements, TRIPS is binding on all member countries of WTO. TRIPS aims at establishing strong minimum standards for intellectual property rights (IPRs). Apart from patents, intellectual property includes copyrights, trademarks, geographical indications, industrial designs, integrated circuits and trade secrets. The protection of IPRs is binding and legally enforceable. Intellectual property (IP) is a product of the mind. Intellectual property is intangible in contrast to real property (land) or physical property, which one can see, feel and use. With any type of property there are property rights. When IPs are expressed in a tangible form, they can also be protected. Intellectual property rights (IPRs) have been created to protect the right of individuals to enjoy their creations and discoveries. In fact, IPRs can be traced back to the fourteenth century, when European monarchs granted proprietary rights to writers for their literary works.