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Pyometra meaning “pus filled uterus” is a condition in cow which is characterized by progressive accumulation of pus in the uterus, presence of persisting CL, absence of estrus and regular estrus cycles and a closed cervix. Pyometra occurs following chronic endometritis in most cases as uterus fails to produce endogenous CL regressing agent i.e. PGF2a. So, the CL of diestrus persists resulting in failure of elimination of uterine infection as the animal remains under the continuous influence of progesterone. Progesterone production from the persistent CL causes closure of cervix, pus accumulation in the uterus and consequently leading to enlargement and distension of uterus.

Uterine prolapse also called casting of the “Calf Bed” in cow is a condition in which there is eversion of whole or part of uterus outside the vulva after its movement through dilated cervix and vagina. It occurs in cow after the birth of calf and mostly in dairy cows having given birth many times to a calf. It occurs due to hypocalcaemia, defective involution, inadequate exercise, excessive force applied during removal of calf etc.

Vaginal prolapse in cow is a condition in which there is protrusion of whole or part of vagina including sometimes the part of cervix through the vulva. It occurs in cow mostly during last 2-3 months of gestation. It occurs due to excess secretion of estrogen from placenta, hypocalcaemia, inadequate exercise, bulky food like roughages etc. Sometimes occurs in non pregnant cows due to cystic ovaries, severe straining due to vaginal irritation etc.

Retained fetal membrane (RFM) in cow is a condition in which fetal membranes or placenta fail to separate and expelled by 12-24 hours after the birth of calf. It is a common in bovines and is usually associated with dystocia, abortion etc. Retained placenta in bovines predisposes the animal to uterine infection and thereby leads to infertility.

Metritis is a condition seen in cattle in which there is the inflammation of the entire thickness of uterine wall (endometrium, myometrium and serosa). When it occurs just after parturition, it is called puerperal metritis. It usually occurs after abnormal birth like in case of dystocia, abortion, RFM etc. The animal shows systemic signs like increase in temperature initially, increase in respiration rate, anorexia, toxemia etc. There is expulsion of large volume of foul smelling reddish to brown discharge from vagina frequently associated with straining. The expelled discharge from vagina is often with pieces of degenerating placenta.

Endometritis in cow is actually the inflammation of endometrium that is inner lining of the uterus. It occurs after bacterial contamination of uterus at calving, during A.I. following dystocia, retention of placenta, abortion etc. There is expulsion of white or whitish-yellow muco-purulent discharge from vagina. The animal does not shows systemic signs like increase in temperature, increase in respiration rate, anorexia, toxemia etc as in metritis. However, the animal fails to conceive even after repeated inseminations.

In subclinical endometritis (SCE), there is inflammation of the internal lining of uterus (endometrium). It is one of the major causes of repeat breeding in cattle and causes significant reduction of reproductive performance. Cervico- vaginal mucus in subclinical endometritis is clear in contrast to endometritis. It is also called cytological endometritis and is associated with increased proportion (percentage) of polymorphonuclear cells (PMN) in endometrial cytology samples. SCE affected cows have delayed first service and subsequent pregnancy causing significant economic losses due to reduction in reproductive performance, feed intake increase per lactation, milk yield reduction and increase in culling rate.

Sub estrus (Weak or Silent Estrus) is a condition in which there are normal cyclical changes in the reproductive organs of a cow but the heat signs are not exhibited to a level of intensity as in normal cows leading to failure of estrus detection by a farmer. This condition is common during post partum period. It is a common reproductive problem in cattle affecting the productivity and economics of a cow due to increase in inter-calving interval, production loss, treatment expenses etc.

Anoestrus in cows is a condition in which there is absence of observable estrus signs. Anoestrus in cattle is suspected when estrus in not expressed by the female animal or in case the farmer fails to detect it. Among reproductive problems, it is one of the most commonly occurring conditions in cattle. It affects the production and economics of a cow to a great extent and the economic losses occur due to increase in inter-calving interval, poor net calf crops, loss of production, expenses incurred on treatment etc. It is caused by multiple factors including mostly inadequate or unbalanced nutrition, environmental stress, uterine or ovarian pathologies and poor managemental practices.

Diagnosis

• Rectal examination of animal. The ovaries are small and smooth. Should be confirmed by repeated examinations at 10 days interval.

• Ultrasound examinations at regular intervals can be done to diagnose and confirm anestrous.

• Progesterone estimations at intervals of 10 days would aid in confirmation. Low progesterone levels at both times indicate true anestrous.

A cow is called repeat breeder when it has failed to conceive even after three or more number of services, has normal estrus cycle length, no abnormal vaginal discharge, no palpable abnormality in the reproductive tract, has calved at least once before and is less than ten years of age. Repeat breeding is one of the most important infertility problem faced by field veterinarians.

Causes

1) Fertilization failure

2) Early embryonic death

Main Points that Need to be Known by a Farmer About Repeat Breeding Cow

• Signs of estrus like vaginal discharge and its color, bellowing, mounting etc

• Time of estrus as observed by owner (morning, evening etc)

• Vaginal discharge whether clear or mucopurulent/purulent

• Duration of estrus

• Inter-estrous interval

• Number of inseminations

Definition of Disaster

A disaster can be defined as a sudden or catastrophic event that causes significant damage, destruction, and disruption to human life, property, or the environment. Disasters can be natural, such as earthquakes, hurricanes, floods, wildfires, or they can be human-made, including industrial accidents, terrorist attacks, or technological failures.

Disasters often result in the loss of lives, injuries, displacement of people, infrastructure damage, and the interruption of essential services like electricity, water, and communication systems. They can have long-lasting impacts on communities, economies, and the environment.

Governments, organizations, and communities implement disaster preparedness, response, and recovery strategies to mitigate the effects of disasters, protect lives and property, and facilitate the rebuilding and restoration of affected areas.

Remote sensing can be a useful tool in engineering geological investigations, particularly in the identification and characterization of geological features and hazards.

Some of the ways that remote sensing can be used in engineering geological investigations include:

Geological mapping: Remote sensing data can be used to create detailed maps of the geological features in an area, including rock formations, faults, and folds. This information can be used to identify potential hazards, such as landslides and rockfalls, and to plan engineering projects accordingly.

Landslide detection and monitoring: Remote sensing data, such as satellite imagery, can be used to detect landslides and monitor their movement over time. This information can help engineers to design and implement appropriate stabilization measures.

Ground deformation monitoring: Remote sensing techniques, such as Interferometric Synthetic Aperture Radar (InSAR), can be used to detect ground deformation caused by geological processes such as subsidence, uplift, and faulting. This information can be used to identify potential hazards and plan engineering projects accordingly.

Remote Sensing in Oil Exploration

Remote sensing plays a significant role in the oil exploration industry. It refers to the use of sensors and instruments to gather information about the Earth's surface and its environment from a distance. This information can be used to locate and evaluate oil and gas deposits.

There are different remote sensing techniques used in oil exploration, including

Seismic surveys: This technique involves creating sound waves underground and recording their reflection to determine the subsurface structure. It is commonly used in oil exploration to locate potential oil and gas deposits.

Magnetic surveys: This technique uses a magnetometer to measure the magnetic field of the Earth's surface. Magnetic anomalies can indicate the presence of underground geological structures that may contain oil or gas deposits.

Gravity surveys: This technique involves measuring variations in the Earth's gravitational field, which can indicate the presence of underground geological structures.

Remote Sensing in Mineral Exploration

Remote sensing is a powerful tool used in mineral exploration to study and identify minerals and mineral deposits from a distance. This technology allows geologists to gather information about the Earth's surface and subsurface without having to physically access the site. Here are some ways remote sensing is used in mineral exploration:

Mapping Geology: Remote sensing can be used to map geology by identifying surface features associated with mineral deposits. Geologists use images collected by remote sensing satellites and aircraft to create detailed maps of the geology and topography of a region. These maps can help identify areas that are likely to contain mineral deposits.

Mineral Identification: Remote sensing can help identify specific minerals by analyzing the reflectance or absorption patterns of light. Different minerals have unique spectral signatures that can be detected by remote sensing instruments. By analyzing these signatures, geologists can identify the type and abundance of minerals present in an area.

Geomorphological mapping is the process of creating detailed maps that illustrate the physical features of the Earth's surface, including landforms, soil types, vegetation, and water resources. These maps can be used to better understand the underlying geology and topography of a region, and can be used for a variety of purposes, such as land-use planning, environmental management, and geological hazard assessment.

Geomorphological maps are typically created using a combination of field observations, aerial photography, and remote sensing data. Field observations involve physically visiting a site and making detailed observations of the landforms, soil types, and vegetation. Aerial photography involves taking high-resolution photographs of the Earth's surface from an airplane or satellite, which can be used to create detailed maps of the area. Remote sensing data can also be used to create geomorphological maps, including data from satellites, radar, and LiDAR (Light Detection and Ranging) systems. These technologies allow scientists to gather data about the Earth's surface without physically visiting the site, and can provide highly accurate information about the topography, geology, and vegetation of an area. The resulting maps can be used for a variety of purposes, including land-use planning, natural resource management, and hazard assessment. For example, geomorphological maps can be used to identify areas of high risk for landslides, floods, or earthquakes, and can be used to develop strategies for managing these hazards.

Rock

A rock is a solid, naturally occurring substance composed of minerals or mineraloids. It is typically formed through geological processes, such as cooling and solidification of molten lava or magma, or by the deposition and compaction of sediment over time. Rocks are classified into three main types based on their origin: igneous, sedimentary, and metamorphic.

Igneous rocks: Formed from the cooling and solidification of molten lava or magma. Examples include granite, basalt, and obsidian.

Sedimentary rocks: Formed from the accumulation and compaction of sediment, such as sand, mud, or organic matter, over long periods. Examples include sandstone, limestone, and shale.

Metamorphic rocks: Formed from the transformation of existing rocks under high temperature and pressure conditions. They undergo changes in mineral composition, texture, and structure. Examples include marble, slate, and gneiss.

Rocks serve as the building blocks of the Earth's crust and are an essential part of the geological processes that shape the planet. They can provide valuable information about Earth's history, including the formation of mountains, the movement of continents, and the presence of ancient life forms. Rocks are also used in various practical applications, such as construction, road building, and the production of minerals and resources.

Structural landforms are landforms that are created due to the underlying geological structures of the earth's crust. These structures can include folds, faults, and other features that have been formed due to the tectonic forces that have shaped the earth's surface over millions of years.

Examples of structural landforms include mountain ranges, plateaus, and rift valleys. Mountain ranges are formed due to the upliftment of the earth's crust along a fault line or due to the folding of the earth's crust. Plateaus are flat-topped landforms that are formed due to the upliftment of a large area of the earth's crust. Rift valleys are formed due to the stretching and pulling apart of the earth's crust along a fault line.

Denudational landforms, on the other hand, are landforms that are created due to the process of erosion and weathering. These landforms are formed due to the gradual wearing down of the earth's surface by natural forces such as wind, water, and ice.

Interpretation of Drainage Patterns Through Aerial Photographs

Aerial photographs can provide a useful tool for interpreting the drainage patterns of a landscape. Drainage patterns are formed by the natural movement of water across the land and the way in which it flows from high to low areas, creating channels and streams that eventually converge into larger rivers and bodies of water. Different types of drainage patterns can be identified through aerial photographs, which can help to understand the underlying geology and topography of the landscape.

Here are some of the different drainage patterns that can be identified through aerial photographs:

1. Dendritic pattern

This pattern resembles the branches of a tree and is the most common type of drainage pattern. It occurs in areas where the underlying rock or soil is uniform and has no preferred direction of erosion. The streams converge into larger rivers, which may flow in a particular direction.

India is a geologically diverse country with a wide variety of rock types. The major rock types found in India can be broadly classified into the following categories:

Igneous Rocks: These rocks are formed from the solidification of molten magma. They can be further divided into two types:

1. Plutonic (Intrusive) Igneous Rocks: Examples include granite, diorite, and gabbro. These rocks are formed deep within the Earth's crust and have a coarse-grained texture.
2. Volcanic (Extrusive) Igneous Rocks: Examples include basalt, andesite, and rhyolite. These rocks are formed on the Earth's surface from volcanic eruptions and have a fine-grained texture.

Sedimentary Rocks: These rocks are formed by the deposition and consolidation of sediments over time. They can be further divided into various types based on their composition and mode of formation:

1. Clastic Sedimentary Rocks: Examples include sandstone, shale, and conglomerate. These rocks are formed from the accumulation and lithification of fragments of pre-existing rocks.
2. Chemical Sedimentary Rocks: Examples include limestone, dolomite, and rock salt. These rocks are formed from the precipitation of minerals from water bodies.

Lithological interpretation of igneous rocks involves the identification and description of the different types of rocks that form as a result of the cooling and solidification of magma or lava. Igneous rocks can be classified based on their mineral composition, texture, and the way they formed. Here are some of the common types of igneous rocks and their characteristics:

1. Basalt

Basalt is a dark-colored, fine-grained rock that forms from the rapid cooling of lava. It is typically composed of minerals such as pyroxene, plagioclase feldspar, and olivine.

Basalt is a common extrusive igneous rock formed from the rapid cooling of basaltic lava. It is typically dark in color, ranging from black to dark gray, and has a fine-grained texture. Basalt is composed mainly of plagioclase and pyroxene minerals, with smaller amounts of olivine, magnetite, and other minerals.

Basalt is found in many parts of the world, including the oceanic crust, volcanic islands, and some continental regions. It is also used as a construction material, such as in road paving, because of its durability and resistance to weathering. Additionally, basalt fiber is used in various applications, including reinforcing materials in composites, insulation, and other industrial uses.

Basalt in India: Basalt is a type of igneous rock that is commonly found in India. It is a dark-colored, fine-grained rock that is rich in iron and magnesium minerals.

Traditionally, remote sensing encompasses a wide array of technologies and methods used to gather information about objects, areas, or phenomena without direct physical contact. This includes devices like seismographs, fathometers, radar systems, and more. These tools enable us to observe and measure various aspects of the environment from a distance.

However, in contemporary contexts, when we talk about remote sensing in a more specialized sense, we often refer to the use of satellite and aerial-based systems to capture and analyze electromagnetic radiation reflected or emitted from the Earth's surface. This form of remote sensing has become incredibly valuable in fields such as environmental monitoring, urban planning, agriculture, forestry, and disaster management, among others.

Modern remote sensing techniques leverage sensors capable of detecting specific wavelengths of electromagnetic radiation, such as visible light, infrared, and microwave radiation. By analyzing the patterns and intensity of this radiation, researchers can derive valuable information about the Earth's surface features, including vegetation cover, soil moisture, land use, and changes over time.

Editing

Editing is the process of refining the content, structure, grammar, and presentation of a written work. Editing is done to make sure that your thoughts are communicated to the reader as clearly as possible.

It takes more than just changing a word, adding a comma here and there, and fixing spelling errors to edit a tale. The procedures below must be followed in order to edit effectively.

1. Read the story from beginning to end first. The writer’s intent will become clearer to you at this point. Then check to see if it addresses the following queries: what? Why? Where? When? Who? and how - regarding the topic.

2. Make your notes in the margin while keeping these inquiries in mind.

3. Next, consider the general level of education and comprehension of your magazine or letter’s readers. Make a note of every word you believe they won’t comprehend.

4. Next, determine how long the story should be. What room in your magazine can you offer it? It is preferable to rewrite the story rather than attempt to condense it by omitting some details if you need a very brief copy.

5. Verify that the information is logically ordered and, if a process story, that the directions are comprehensive and given in the right sequence.

Cutlines, another name for photo captions, are a few lines of text that provide context or additional information for published images. In some circumstances, captions and cutlines are distinct from one another. A caption is a brief (often one-line) title or explanation for the picture, whereas a cutline is a longer, prose block that describes the picture more thoroughly, provides context, or connects it to the article.

The first things people read in a publication are frequently the photo captions. A crucial component of a news photographer’s job is writing captions for photos. The reader should receive the essential information from a photo caption in order to comprehend the image and its news value.

Caption writing should adhere to the same professional standards of clarity, accuracy, and completeness as any other writing that appears in a publication. A lousy caption can lessen the impact of a beautiful photo and damage the caption’s reputation as journalism by being uninformative or, worse, misleading. Why should readers believe the rest of the publication if they can’t trust the accuracy of the straightforward information in a caption?

A decoration, interpretation, or visual explanation of a text, concept, or process that is intended to be included in published media including posters, flyers, magazines, books, teaching materials, animations, video games, and films is called an illustration.

Using Illustrations

We can comprehend information better when it is shown. Illustrations were one of our first forms of communication, long before any kind of contemporary writing. In reality, new discoveries of cave drawings from more than 35,000 years ago show that humanity have been using images to communicate for a very long time. This did not occur by accident; according to study, over 90% of the information we process is visual, and a significant portion of our brain is dedicated to visual processing.

Benefits of using Illustrations

The ease with which a text can be read is referred to as readability. Complexity, recognizability, legibility, and typography are all possible inclusions. Sentence length, syllable density, and word familiarity are common considerations in readability algorithms.

It refers to how easily a reader can comprehend a written content. The complexity of a text’s vocabulary and syntax, as well as how it is presented, determine how easily it may be understood in natural language (such as typographic aspects like font size, line height, and line length).

Why is readability important?

Fundamentals of writing farm stories

1. Use of plain and simple language: A farm journalist would have translated technical terms into their plain language.

2. Presentation of novel ideas: People are open to new concepts that are logically explained and will increase their return on investment as a whole.

3. Keep the message brief and to the point so that farmers can recall it. The information should be focused on the benefits that can be attained without using an excessive amount of wording.

4. Use of visuals: Visuals add interest, are more memorable, and have deeper significance. A statement is more understandable and pleasing to the eye when there are more visuals present.

5. Farmers are very receptive to messages that are recent, reliable, realistic, and relevant (the “4Rs”).

6. The audience appreciates and accepts the message that is centred on their environment, locations, and selves.

• A title should be able to talk. It ought to make a point that both informs and amuses the audience.

• A headline needs to be concise and sum up the story.

• A headline should engage readers and entice them to read the material behind it.

• Make use of recognisable acronyms.

• Assist in setting the publication’s general tone.

• The headline ought to be assertive and upbeat.

• Function as a visual component on the page.

Choosing and designing news headlines

• If necessary, the headline should be written in all capital letters.

• Double-tick the letter that needs to be capitalised.

Sources of information

News is constantly occurring. There is a newsworthy event happening somewhere in the world every minute of every day.

People, letters, books, files, videos, cassettes, and other materials thatjoumahsts utilise to put together news articles can all serve as sources of information.

Types of sources

Primary sources: A person with first-hand knowledge of a problem or a document they produced can both be considered primary sources.

They are typically the best sources of knowledge regarding their particular aspect of what occurred. They should be able to provide you with reliable information as well as powerful opinions.

Example: Artifacts, audio recordings, diaries, internet communication, interview, letters, peer-reviewed journal articles, original documents, patents, photos, proceedings, records of organizations, speeches, videos, survey results, works of art, web sites.

Types

• News story

• Feature story

• Success story

• Popular article

• Radio and TV script

• Process or utility feature

• General information feature

• Experience feature

• Personality feature

News story

• Any new information, idea, event, situation or development, that is of interest to a large number of people, is a news.

• Any event when reported becomes a news.

The term “communication media” describes the channels through which data or information is sent and received. These tools or channels for data storage and transmission are known as transmission and storage means in telecommunication.

It is the effective weapon in today’s environment that eliminates societal ills and anomalies.

Popular types of communication media

Television: Transmits and receives moving images that can be monochrome (black-and-white) or coloured, with or without accompanying sound. Audiovisual medium of communication

Radio: Uses electromagnetic waves with frequencies lower than visible light to modulate and transmit signals. Modulation in electronics is the process of changing one or more characteristics of the carrier signal, a high frequency periodic waveform, in relation to a modulating signal.

Definition

Agricultural journalism is a specialized branch of journalism that deals with the techniques of receiving, writing, editing and reporting from information through the media like newspapers, periodicals, radio, TV, advertising etc. and the management processes connected with such production. It is the timely reporting and editing with words and photography of agricultural news and information for newspaper, magazine, radio and television.

It is otherwise known as Farm journalism

Farm journalism is very important for communicating with farm people. More and more of the extension staff’s writing is being read or listened to in newspapers, magazines, brochures, pamphlets, bulletins, circular letters, wall newspapers, and radio scripts. The written work makes working as a consultant with farmers more fascinating.

Principles of Farm/Agricultural journalism

The word journalism is derived from the word “Journal” which means a daily register or a diary.

• Journalism is the written, spoken, or visual dissemination of information about a day’s events.

• A journalist is someone who works as a writer or editor for a newspaper or magazine.

• Father of journalism: Joseph Pulitzer

• Father of journalism in India: James Augustus Hicky

Mesothelioma

• Arising from the mesothelial lining of serous cavities
• It is very difficult to differentiate from carcinoma, reactive mesothelial hyperplasia
• Round to polygonal cells
• Arranged mostly in clusters
• Centrally located hyperchromatic nuclei

Hepatocellular Carcinoma

• The presence of normal appearing hepatocytes of atypical hepatocytes with malignant characters
• Single to clusters of polyhedral or polygonal shaped or pleomorphic cells
• Large round, spherical or oval nuclei
• Higher nuclear cytoplasmic ratio
• Large nucleoli
• Dark blue cytoplasm

Adrenal Tumours

• Adrenal cortical adenoma had normal secretory cells
• Most cells had naked nuclei in the abundant cytoplasm
• Basophilic cytoplasm with abundant clear lipid vacuoles
• Round uniform nuclei

Canine Thyroid Tumours

• Occurs as carcinoma
• Blood contamination also seen
• Cluster of cells
• Free nuclei seen in the pale-blue cytoplasm
• Dark blue to black pigment present in the cytoplasm
• Amorphous pink materials represents colloid
• Colloid and pigmented granuls indicates the thyroid origin
• Round to oval nuclei

Testicular Tumours

Seminoma

• Cells were round exhibiting anisocytosis
• Round to oval nuclei
• Single distinct eosinophilic to basophilic nucleoli
• Cytoplasm scanty

Sertoli Cell Tumours

• Cellularity - High, anisocytosis
• Nucleus round to oval, megakaryosis, 1-3 basophilic nucleoli
• Chromatin coarse and granular
• Cytoplasm vacuolated

Mesenchymal Tumours

General Features

• Do not exfoliate many cells - hypocellular; Low cellularity
• Embedded in extracellular matrix-osteoid, chondroid or collagen
• Single, discrete cells and a few are also seen in dense clumps i.e. Individual or unorganized cluster of spindle cells
• Cells-Elongated, fusiform/spindle shaped
• Cytoplasmic extensions/tails seen on both ends of nucleus; borders ill-defined
• Nucleus elongated/fusiform to oval
• As the malignancy potential increases cells become plumpy with a larger, rounder nucleus and shorter cytoplasmic tails
• Nuclei may be fusiform or oval.
• These tumors cannot be differentiated cytologically.
• Tentative diagnosis made.
• Well-differentiated malignant mesenchymal tumors may resemble actively proliferating granulation and requires histologic confirmation.

Non-Neoplastic Conditions

Haematoma

Collection of blood in outside of the blood vessels It is more common in external ear

• Presence of erythrocytes, blood filled leococytes (non-degenerative neutrophils and macrophages) and platelets
  • Haemosiderin or haematoidin crystals will be seen

Epidermoid cyst/Epidermal cyst/Epidermal inclusion cysts/Follicular cyst

Cytologically cannot distinguish epidermoid cyst from dermoid cyst

• Constitutes ????V3 to V2 of non-neoplastic/non-inflammatory lesion
• Smear is thick
• Keratin bars, squames and other keratinocytes

Points to Ponder

Collect cytological samples (FNAB) only from enlarged lymph nodes

Do not make attempt to collect samples from normal lymph nodes unless to study normal picture (Academic interest).

Care to be taken to hold the lymph node while collecting FNAB

Otherwise needle may go into perinodal fat yielding fat tissue so also in obesity

Submandibular salivary gland enlargement mimic submandibular lymph node enlargement

Salivary tissue will show epithelial cells, acinar formation and no lymphocytes

Cytological diagnosis of lymphadenopathies can be comfortably and confidently made.

Peripheral Lymph Nodes

Discrete Cell Tumours

(Round / discrete / distinctive / unique cell tumours)

General Features

• Exfoliate many cells
• Cells often quite small to medium sized
• Discrete cells
• Do not stick together
• If the smear is very cellular, cells are packed close together in sheets
• However, these cells are not adherent to one another
• There are sharp, crisp cytoplasmic margins between cells

Answer Inflammatory or Not

• If yes it is non-neoplastic or inflammatory
• If no, consider neoplastic conditions
• Certain non-neoplastic conditions may not show inflammatory cells (Haematoma, cystic fluid, epidermal/dermal cysts)
• Inflammation or non-neoplastic condition is characterized by a mixed population of cells including neutrophils, lymphocytes, plasma cells, eosinophils, macrophages, epithelioid cells and giant cells.
• Inflammatory reactions often result in hyperplasia of surrounding tissues.
• If not inflammatory, it may be neoplastic.

Good smear is well spread and tongue shaped. If forced during discharge of materials from the syringe, it is sprayed on to slide.

The ideal smears will yield intact and well stained cells (Figure) should have a clear demarcation between the nucleus and cytoplasm

Poor smear will show no cellularity or damaged cells.

Location is important

Since, one should recognize whether cells belong to that area and / or not

Basic aim

• To establish an aetiological and/or morphological diagnosis in order to obtain a more accurate prognosis

Salient Points in Cytological Examination

• Cytology is a joint effort between the clinician and cytologist
• Adequate history, clinical signs and methods of collection should be intimated
• Interpretation suffers if done in a vacuum of information
• Cytology is an rapid, inexpensive technique in tumour diagnosis

Check the Following

Different types of Romanowsky type of stains are used for staining the cytological smears. It will give excellent cytoplasmic and nuclear details and it also stains bacteria. The following stains are commonly used. e.g., Wrights, Giemsa, Leishman, Wright-Giemsa, May-Grunwald-Giemsa, Leishman-Giemsa, Haematoxylin and Eosin, Diffquik stains, Trichrome stains, Papanicolaou or Sano modification.

Staining Methods

Leishman-Giemsa

• Cover the smear with LG stain for a minute
• Dilute with tap water/Distilled water/ phosphate buffered saline pH 6.87.2
• Allow to stain for about 20 min
• Wash in water

Fixation can be done by either wet fixation or dry fixation. If staining is to be delayed for a few days, cell preservation enhanced by fixation. Fix the slide within 30-60 seconds.

Wet Fixation

A judicious use of cytology will be beneficial to the practicing veterinarians as diagnosis is available within a shortest period of time and can decide on proper treatment e.g. TVT can be successfully treated medically without surgical intervention.

Diagnostic Approach in Clinical Set Up

• Clinical investigation
• Clinical pathology
• Necropsy
• Biopsy
• Cytology
• Histopathology
• Electron microscopy
• Fluorescent antibody techniques
• Immunohistochemistry
• Genomic techniques
• Microarray
• Proteomics
• Metabolomics etc.

Cytology is the study of cells from fluid or tissue samples prepared directly on glass slides and stained for microscopic evaluation. It is also described as examination of cells without regard to the tissue's architectural details.

It is less invasive than tissue biopsies and, in some cases, it can provide a rapid diagnosis that would otherwise take several days if done by histopathology.

When is the cytology needed?

The diagnostic methods of clinical examination, radiographic evaluation, and laboratory data suggested that a diagnosis that cannot be confirmed by these methods. In that situation, cytology is very much helpful to confirm the disease processes or conditions.

Introduction

Lac, a resinous exudation from female scale insects, has been utilized in India since the Vedic period. Its earliest mention can be traced back to the Atharva Veda, where the insect is referred to as 'Laksha,' and its characteristics and behavior are described. In the renowned Indian epic 'Mahabharata,' there is also a mention of a 'Laksha Griha,' a house made of lac, skillfully constructed by the 'Kauravas' with the help of their architect 'Purocha,' with the malicious intent of setting the 'Pandavas,' their formidable enemies, on fire. The use of lac was well-known to the Greeks and Romans since ancient times. The use of lac was well-known to the Greeks and Romans since ancient times. In Asia, the cultivation of lac insects has a rich history, with indications that it dates back as far as 4000 years in China, where its cultivation coincided with the growth of the silk industry