eChapters

Introduction

Diagnostic biochemistry, is a branch of laboratory medicine that focuses on the analysis of bodily fluids to diagnose and monitor diseases. This field plays a crucial role in understanding the biochemical mechanisms underlying various conditions, enabling accurate diagnosis, prognosis, and management. The biological samples for diagnosis include blood, urine, cerebrospinal fluid, pleural fluid and tissues.

Factors Affecting Serum Enzyme Activity or Protein Level

1. Organ Mass and Enzyme Tissue Concentration:

• Higher enzyme concentrations in organs can lead to greater increases in serum enzyme activity during disease. For instance, the liver’s large mass contributes to potential increases in serum ALT activity.

2. Cell Location

• The proximity of cellular enzymes to the blood, urine, or other fluids determines the fluid in which an enzyme’s activity will increase upon cell injury. For example, renal tubular gamma-glutamyltransferase (GGT) is released into urine, not blood, following injury.

Diagnostic biomarkers are measurable biological molecules that provide information about physiological or pathological conditions. This chapter examines the use of metabolomics for discovering, evaluating, and validating biomarkers for disease diagnosis, prognosis, therapeutic monitoring, and personalized medicine. It discusses different types of biomarkers, metabolomic biomarkers, biological samples, analytical platforms, biomarker-discovery workflows, and statistical analysis. Applications in cancer, diabetes, cardiovascular, liver, kidney, neurological, infectious, and metabolic diseases are considered. The chapter also explains the role of metabolomics in drug discovery, toxicology, nutrition, and clinical research. Challenges involving biological variability, analytical reproducibility, data complexity, and clinical validation are addressed alongside future opportunities involving AI and multi-omics.

Diagnostic techniques are routinely used for diagnosing different surgical conditions. The most frequently used imaging techniques in veterinary practice are radiography and ultrasonography. Radiography and ultrasonography are not suitable for each organ in large animals. In small animals most organs are suited to ultrasound images except bones and gascontaining structures such as lungs etc. Bone and gas reflect ultrasound and do not allow the sound waves to form image therefore radiography gives better diagnostic images for these organs. The liver, spleen, kidneys, prostate, gravid uterus and fluid containing organs like urinary bladder, gall bladder and major blood vessels can be better imaged with ultrasound. In buffaloes in surgical section important clinical conditions relate to urinary and digestive systems, mammary gland and perineal area.

1. Urinary System: In bovine, the cases of obstructive urolithiasis are commonly observed. These types of cases are more common in bullocks and in buffalo calves. In these cases radiography is not so useful. Sonography can be tried to take the image of ruptured urethra in bullock and can be compared with radiography. Due to ruptured urethra there will be accumulation of urine in the s/c tissue of prepuce. Due to fluid density the image will be radioopaque and will appear white and no detail of the organ can be seen. The ultrasonogram (USG) will show images of glans penis and its coverings. Different diameters of urethra and a point of rupture just behind to glans penis can be make out. Due to obstruction, The B.calves either will pass drop wise urine or there will be complete stoppage of the urine. These cases can also be easily diagnosed by ultrasonography.

The aquaculture sector in India is expanding rapidly, playing a critical role in enhancing rural livelihoods, ensuring food security, and boosting export revenue. However, disease outbreaks remain a persistent hurdle, often leading to substantial financial losses for fish farmers. To mitigate this, the deployment of user-friendly, efficient, and rapid diagnostic tools at the field level has become increasingly vital—especially in areas with limited access to laboratory facilities.
Fish disease diagnostic kits, based on immunological and molecular technologies, are designed to detect specific pathogens responsible for viral, bacterial, parasitic, and fungal infections. A major benefit of these tools is their ability to deliver results swiftly—typically within minutes or hours—enabling prompt action. In India, research bodies such as ICAR-CIFA, ICAR-CIBA, and ICAR-NBFGR have played a key role in developing and adapting these tools for local aquaculture conditions.
Efficient nucleic acid extraction is a cornerstone of field diagnostics. Rapid DNA/RNA extraction kits that function without centrifuges and under varied environmental conditions have made sample processing quicker and easier. Mobile diagnostic vans—equipped with portable lab equipment—have also been introduced in states like Andhra Pradesh and Tamil Nadu for on-site disease surveillance and farmer training.
For detecting parasites, wet mount kits containing preloaded reagents like iodine and saline are used for direct microscopic examination of fish tissues. These kits help identify parasites such as Trichodina, Ichthyophthirius, and monogeneans, aiding in immediate diagnosis and treatment advice.

12.1. Introduction

The protocols provides guidance on the structure and content of the International Plant Protection Convention (IPPC) diagnostic protocols for regulated pests. The protocols describe procedures and methods for the official diagnosis of regulated pests that are relevant for international trade. They provide at least the minimum requirements for reliable diagnosis of regulated pests.

This standard sets the framework for the content of diagnostic protocols, their purpose and use, their publication and their development. Diagnostic protocols for specific regulated pests are included as annexes to this standard. Information relevant for diagnosis is provided in the diagnostic protocol on the specified regulated pest, its taxonomic position, and the methods to detect and identify it. Diagnostic protocols contain the minimum requirements for reliable diagnosis of the specified regulated pests and provide flexibility to ensure that methods are appropriate for use in the full range of circumstances.

The methods included in diagnostic protocols are selected on the basis of their sensitivity, specificity and reproducibility, and information related to these factors is provided for each of these methods. Detailed information and guidance for the detection of pests is provided on, for example, signs and/or symptoms associated with the pest, illustrations (where appropriate), developmental stages of the pest, and methods for detecting the pest in a commodity, as well as methods for extracting, recovering and collecting the pests from plants.

Introduction

To maintain optimum reproductive status early diagnosis of reproductive problems is important. Various advance diagnostic techniques are available for diagnosis of reproductive problems. Some of them are still in early stages of development or some though available; are not widely used due to various constrains.The various diagnostic techniques used in animal reproduction are ultrasonography, Doppler ultrasonography, endoscopy, laparoscopy, hormonal assays, vaginal cytology, x ray etc.

Accurate history and Thorough examination of reproductive tract

Accurate history is a powerful tool of enormous clinical value. Behavioral problems due to pain or psychological factors can also be ruled out on the basis of adequate history of the patient. Tentative differential diagnosis can be made by taking history of previous breedings.Through digital and vaginiscopic exanimation of reproductive tract helps to rule out anatomical abnormalities, which may interfere with natural mating. It also helps in diagnosis of fertile phase of oestrus cycle. In cases of refusal to mate due to anatomical problems like septum, narrow passage or due to behavioral problems AI can be performed. Vaginoscopic examination aids vaginal cytology in deciding right time of natural service or insemination in proper breeding management or abnormal oestrus cycle.

Any abnormal condition, which affects the normal functioning of cells, tissues, organs and any other system in an individual, is called a disease. In all living organisms, including plants, animals, birds and humans, diseases can be caused due to infectious or non-infectious agents and various other factors. Laboratory tests may identify organisms directly (eg, visually, using a microscope, growing the organism in culture) or indirectly (eg, identifying antibodies to the organism). Some tests (eg, Gram stain, routine aerobic culture) can detect a large variety of pathogens and are commonly done for many suspected infectious illnesses. However, because some pathogens are missed on these tests, clinicians must be aware of the limitations of each test for each suspected pathogen. In such cases, clinicians should request tests specific for the suspected pathogen (eg, special stains or culture media or advise the laboratory of the suspected organism(s) so that it may select more specific tests. The main causative factors of diseases in humans include internal factors, genetic irregularities, allergies, poor immune system, external factors and a lot more.

Several diagnostic techniques are employed to asses the nutrient status of the crop. These are helpful in determining specific or multinutrient stresses and quantity of nutrients needed to eliminate the stress. Some of the techniques commonly used are:

Visual deficiency symptoms

Plant analysis

Rapid tissue testing

True /False

Q.1.    A diagnostic test enables the doctors to reach at a decision whether the patient is suffering from a particular disease or not.
Q.2.    The recent developments in the field by biotechnology have led to the development of many new medical tests.
Q.3.    The general principles in designing and conduct diagnostic test studies are not known well.

X-ray Machines
 
There are many factors to consider when choosing X-ray equipment. The need of an individual practice varies depending on the species majority and case work load.
Types of X-ray machines: 
X-ray machines can be grouped into 3 categories:

I. Portable X-ray Machine

    ·    Is one that can be carried to the animal.
    ·    Portable X-ray machines are widely used in veterinary practice.
    ·    The maximum output is 15 to 35 mAs and kV ranges from 70-110 kVp.
    ·    Cost is less and easy to move around and transport. 
    ·    Suitable for X-raying of thin and small parts (limbs below stifle and elbow of large animals and can be used taking 
X-rays of abdomen an skeletal systems of small animals).

Section-A : Write True / False

Q.1 A better and more beautiful way to present the data in a more visual form can be done by means of diagram.

Q.2 Description of data through diagrams and graphs is better for layman.

Q.3 Diagram does not register a meaningful impression almost before we start to think about it.

Q.4 Diagrams are never used by the business man and government agencies for advertisement and educating people.

Q.5 Diagrams do not give visual presentation of data.

Q.6 The diagrams put a rememberance for a longer time in mind of human being.

Q.7 Diagrams facilitate comparison and reveal pattern of data.

Q.8 Diagrams show approximate values of items in data.

Q.9 Diagrams are more attractive, appealing and impressive than tables.

Q.10 Diagrams can be more easily compared and interpreted than the tables.

Q.11 Tables contain precise figures while diagrams indicate approximate figures.

Diagrams and graphs are the effective and attractive ways of presenting data. Widely used diagrams for data presentation are pi chart and bar diagrams. Bar diagrams are also of various types; (i) Simple bar diagram (ii) Component or sub-divided bar diagram (iii) Multiple bar diagram (iv) Percentage bar diagram. The widely used graphical presentation are histograms, frequency polygon and frequency curve.

There are numerous libraries to create charts and graphs in R programming language.

Pie-chart- A pie-chart is a representation of values of variable as sectors of a circle with different colours/shadings. The colours/shadings used for different sectors are indexed at the side of the graph. pie() function is used to create pie chrt in R.

Let us create an exel file names as ‘exp_sports’ which contains the expenditure made (in Rs.) under various sports in a sports club. The name of the sports are cricket, hockey, tennis and football. The names of sports are placed under the column head ‘sports’. The corresponding expenditure (in Rs.) are 7200, 3600, 1800, 1800 and they are placed under the column head ‘expenditure’.

The basic syntax for creating a pie-chart using the R is.

Diagrammatic Presentation of Data

The quantitative data when classify are converted into averages and if the number of average is large then it is not possible to draw a conclusion from their large numbers of averages. Moreover quantitative data are dull, confusing, boring for many of us. Therefore, a statistical device to make such confusing data more attractive was developed i.e. if such complex data are presented by geometric f igures, pictures, graphs or curves then it will become more attractive and more impressive, is called as diagrammatic representation of data. These diagrams are nothing but the use of geometrical figures to improve the overall presentation and offer visual assistance for the reader.

Types of Diagrams

Generally, diagrams are made for a special series or a time series and they are divided into five different categories:

1) One dimensional diagrams

2) Two dimensional diagrams

3) Three dimensional diagrams

4) Pictograms

5) Cartograms

4.1 Diagrammatic Representation of Data

Diagrams are meant only to give a pictorial representation of the data with a view to make them readily intelligible. Several types of diagrams are used for the presentation of data in biological and agriculture sciences. The following are the important types of diagrams in common use :

1. One-dimensional diagrams
2. Two-dimensional diagrams
3. Three-dimensional diagrams

Introduction
Plant breeding, according to Sansern et al. (2010), is the process of genetically altering plants to meet human demands. Prehistoric humans in various parts of the world domesticated only a small number of the hundreds of thousands of species that were then available. They did this by genetically altering these species’ components, which entailed both conscious and unconscious selection as well as differential reproduction of variants (Allard, 1999). A relatively small number of plant species have evolved over many years of trial and error to become the backbone of agriculture and, consequently, the world’s food supply (Mackay, 2001). This method of domestication includes the identification of specific valuable wild species along with a system of selection that resulted in alterations in the quality and quantity of cultivated crops.The modifications brought about by domestication involved altered organ size and shape, loss of many survival traits, such as bitter or toxic substances, the disintegration of seeds in grains protective structures, such as spines and thorns, seed dormancy, and change in life span increased for crops grown for roots or tubers and decreased for crops grown for seed or fruit. The domestication process followed mostly by mass selection (selection by bulking preferred kinds) is a potent method for facilitating quick changes while preserving genetic variability in the population (Okporie and Obi 2002).
Populations can exist in constantly changing surroundings due to genetic variation. More variation increases the likelihood that certain individuals in a group will have allele variants that are appropriate for the environment. Those individuals have a higher chance of survival to have offspring which carry that allele. Because of their success, the population will endure for additional generations. Selecting the best parents to utilize in artificial crosses is one of the key concerns for plant breeders since it encourages the expression of the largest genetic variability and the production of superior recombinant genotypes. The objective of plant breeding is to simultaneously improve all the desired traits. This task is complicated by correlations between various traits, which may result from pleiotropic genes, physical chromosomal links between genes, or population genetic structure. Choosing one feature will alter associated qualities, sometimes in a positive or in a negative way. As a result, selection may result in unexpected modifications, provided they fall within the typical range of variations seen in the crop and are therefore thought to be useful to consumers or the environment. Breeding practices in the 20th century improved a variety of genetic techniques, including methods for identifying and preserving more genetic diversity, creating variable populations for selection, and testing how to differentiate between the effects of genes and the environment. Naturally self-pollinating plants like soybeans or tomatoes, naturally cross-pollinated plants like maize, and plants whose vegetative propagation (typically cross-pollinated) allowed the direct fixing of improved types, all required different precise steps in the production process (Ubi, 2013).

Dialysis
 
The main purpose of dialysis in biological research is the removal of small molecular weight (M.W.) constituents from biological fluids. This is achieved by dialysing the material in a cellulose tubing with a pore size of 40-80 A0 (4-8mm) diameter which allows the passage of compounds with molecular weight less than 10,000. The following is the procedure followed: cut the length of the dialysis tubing depending upon the volume to be processed. Open the sides of the tubing with a flow of distilled water and transfer it to a boiling water bath. Do not allow the tubing to dry as it will distort the pore size. Make a bag by tying a knot at one end of the tubing. Transfer the solution to be dialysed into the bag. Tie another knot at the top of the bag. The bag is then put in a beaker containing the solution against which dialysis is to be carried out. In general, one volume of the desired solution is dialysed against 50-100 volumes of buffer. The total period of dialysis could range from 8-48hr. The efficiency of dialysis could be increased by stirring and changing the dialysate solution at frequent intervals.

Key Points

• Neonatal diarrhea can be lethal without treatment.

• Administer aggressive fluid and electrolyte therapy to address newborn diarrhea.

• Fecal examination and/or culture findings can help control diarrhea.

• Ringer lactate is the preferred fluid.

• There is a high association between animal age and the presence of the causal factor for diarrhea.

Introduction

Diarrhea is one of the symptoms of a variety of medical problems. Diarrhea is characterized by an increased frequency of defecation and stools of varied consistency, which may have an increased concentration of water or a decrease in dry matter content. Increased peristaltic movement causes an increase in caudal flow of ingesta/GIT content, resulting in a decrease in intestinal transit time and thus diarrhea. Diarrhea is lethal in children; therefore, preventing f luid and electrolyte losses prior to diagnosis is critical to saving lives.

Many non-spore-forming, anaerobic, Gram-negative bacteria cause opportunistic mixed infections, often in association with facultative anaerobes. Synergistic interactions between the organisms in these mixed infections are common. Fusobacterium species and bacteria formerly referred to as Bacteroides species account for more than 50%of the anaerobic organisms isolated from these infections.
Usual Habitat
Nan-spore-forming, Gram-negative anaerobes are often found on muwus membranes, particularly in the digestive tract, of animals and man. They are excreted in the faeces and they can survive for short periods in the environment. Dichelobacter nodosus, a primary pathogen of the epidermal tissues of the hoof region of ruminants, survives for less than 4 days in mud. Diagnostic Procedures
In order to ensure that isolates of anaerobes are aetiologically significant, specimens for isolation procedures should be obtained by direct sampling from discharges or lesions and by supra pubic puncture in urinary infections. Specimens should be processed promptly after collection. Commercial kits and transport media are available for specimens from suspected anaerobic infections. In the core of a tissue specimen over 2-3 cm, an anaerobic microenvironment is usually maintained. Samples of fluid in a syringe remain suitable for anaerobic culture if air is expelled from the syringe and the needle
is plugged.
Anaerobic jars with an atmosphere of hydrogen and 10%CO2 are used for incubating cultures at 37°C for up to 7 days.

8.1 Introduction to Dictionary in Python

A dictionary is a collection which is unordered changeable and index. In python dictionaries are written within curly brackets, and they have keys and values. Means the dictionary contains two things first is the key and the second is the value.

8.1.1 Create and Print a Dictionary

Example

dict1={‘brand’:‘Suzuki’,‘model’:‘dzire’,‘year’:2020}

print(dict1)

Accessing items: you can access the items of a dictionary by referring to its key name, inside square brackets.

Example

Accessing items: there is also a method called get() for the same task.

dict1={‘brand’:‘Suzuki’,‘model’:‘dzire’,‘year’:2020}

print(dict1)

y=dict1.get(?model”)

print(y)

Abstract

The electromagnetic processing instruments such as microwave is very commonly used at house hold level, hence understanding the behavior of food as well as factors affecting its dielectric properties becomes important. Dielectric properties (DEP) play an important in electromagnetic processing of food products and mainly depend upon the moisture content, physical properties, composition, temperature and power applied. Different methods have been used to determine the DEP of food material including parallel plate, coaxial probe, resonance cavity, transmission line and free space method. The selection of these methods is again based on several factors such as type of materials, accuracy of measurement, the scope of the research, frequency range, availability of equipments and resources. Present study thus summarizes the importance of DEP of food, influencing factors and the different measurement techniques.

Diet plays an important role in the management of diseases. It is very much true in the management of dogs with heart failure also. Managing the diet of a dog with heart failure is depending on the severity and stage of heart failure and presence / absence of concurrent diseases as well deficiencies.

Introduction

Fiber is that part of plant food which is not digested when eaten. Since it is not absorbed into the body, dietary fiber is not a nutrient. Nevertheless, fiber is a very important component of a healthy diet. Fiber, also called “roughage” helps prevent constipation, hemorrhoids and diverticular disease. Some types of fiber have a cholesterol-lowering effect, which could lead to reduced risk of heart disease.

Dietary fiber has been defined as plant cell wall material resistant to the intestinal secretions of the host. This definition gives the appearance of precision and allows gravimetric and chemical measurements. However, a proportion of other non-fibre dietary constituents (e.g, retrograded starch, protein, and fat) pass along the gut, without absorption or metabolism, to the caecum. Chemical analysis or quantitative measurements of the fibre content of specific diets do not allow prediction of their biological action. The physiological effects of dietary fibre depend predominantly on physical properties unrelated in any simple or direct way to chemical composition. Quantitative measurement of dietary fibre does not allow for diverse effects on nutrient absorption, sterol metabolism, fermentation in the colon, and stool weight. We still do not know whether to restrict the definition of “dietary fibre” to non-starch polysaccharides or to include related materials such as resistant starch, lignin, and the complex assemblies typical of plant tissues, where polysaccharides occur in close association with other molecules. An alternative to defining fibre in terms of chemical composition would be to develop measurements of physical properties relevant to physiological action in the intestine. This approach could be extended to other polymeric constituents, in particular proteins (ingested and secreted) and mucous glycoproteins.

Polysaccharides are the main constituents of dietary fibre and show a wide spectrum of physical, properties that reflect the nature and extent of intermolecular association. Dietary fibre can be likened to a water-laden hydrated mass passing along the gut. Physical properties that influence function are a combination of the rheological and colligative properties of the water-soluble fibre components, the surface characteristics of the water-insoluble components, and the network properties of the swollen hydrated components. Such features include viscosity, water holding, cation exchange, organic acid adsorption, gel filtration, and particle size distribution.

Introduction

In India, per capita cereal consumption has been declining since the early seventies, despite a significant increase in per capita cereal production (Radhakrishna and Ravi, 1992 and Rao, 2000). Cereal consumption per capita per month fell from 15.35 kgs in 1970-71 to 12.70 kgs in 1999-2000 in rural area and from 11.40 kgs to 10.40 kgs in urban area during same period. It might be due to shifting of consumers’ choice from food to non-food items; from cereals to non-cereals within the food group and from course to fine cereals within the cereal group. The decline in cereal consumption has been higher in the rural area due to development of rural infrastructure and availability of job opportunities which ultimately helped in improving their level of income (Rao, 2000). Despite spectacular growth in food production during last 30 years the problem of malnutrition still persists across different regions in India because increase in food grain supply alone cannot conquer hunger and malnutrition.

The consumption pattern and nutritional status have also experienced a significant change during last two decades in Bihar. Cereal consumption per capita per month has declined from 476 gms in 1983 to 455 gms in 19992000. The situation is more alarming in poor households who constitute about half of rural population in north Bihar. There is lack of research on consumption pattern, nutrient intake and nutritional status based on household data, particularly in context of Bihar. In view of the above observations the present study is undertaken to analyze the consumption pattern and nutritional status of poor households in north Bihar.

Abstract

The concept of functional foods has received significant media attention and, as a result, interest has focused on the need for research into the non- nutrient constituents of food plants. This has enabled market penetration of functional foods containing increased amounts of bioactive food compounds or physiologically active nutrients and non-nutrients within food that impart proven health benefits. Functional foods can take many forms. Some may be conventional foods with bioactive components that can now be identified and linked to positive health outcomes. Some may be fortified or enhanced foods, specifically created to reduce disease risk for a certain group of people. Numerous observational studies have shown that omega-3 fatty acids enriched diets are associated with a reduction of cardiovascular mortality, heart attack and sudden death. In several studies, a higher fish intake has been associated with decreased incidence of coronary artery disease (CAD) and cardiovascular mortality. A trial revealed that fish oil supplementation of 130 mg/kg of body weight each day decreased the number of stiff joints, duration of morning stiffness, pain and global arthritis activity. Many supplements contain active ingredients that have strong biological effects in the body. The bioavailability of nutrients within a given food is governed by the composition and microstructure of the food, commonly referred to as the food matrix. More specifically, numerous studies investigating the development of functional foods have shown that the interactions between nutrients and non-nutrients within the food matrix can be synergistic, additive, or neutralizing in nature. The preventive and curative effects of the functional foods on health concludes it to be integral components of established public health programs to reduce the risk of specific diseases.

7.1 Introduction

In this chapter, process, outcome and impact evaluation of the vegetable integrated pest management programme (IPM) implemented in the subtropical area of the Jammu region of Jammu and Kashmir (J&K) state during 2008-09 and 2009-10 was studied. There are many research designs employed for impact evaluation. These include one group before and after comparison design, one group time-series design, non-equivalent control group design and ex-post facto research design. Social scientists most often used before and after IPM (longitudinal) comparisons, with and without IPM, and control group (latitudinal) comparisons, and a combination of the two (longitudinal and latitudinal). A robust measure of the impact of a programme, however, is the difference-in-differences (Ashenfelter and Card, 1985) quasi-experimental design, a statistical technique used in econometrics and quantitative sociology to determine the difference between the treatment (with) and control group (without) over time. The difference-in-differences quasi-experimental design, also called double difference (DD), used to compare the treatment (with IPM) and control group (without IPM) over time. The objective of our quasi-experimental empirical study was to determine the impact of the vegetable IPM programme on the adoption of non-chemical pest management practices, pesticide use, and field use environmental impact in the sub-tropical region of the J&K state. Difference-in-difference quasi-experimental control group design (with/without, before/after) was used to compare the changes before and after the IPM programme between the IPM trained farmers and the non-IPM farmers. The DD model was employed to identify the impact of the IPM programme on the adoption of non-pesticide IPM practices, pesticide use by weight (a.i.), pesticide use frequency, farmers’ knowledge regarding safe use and handling of the pesticides, and field use environmental impact to address the bias introduced by a non-random sampling procedure as the farmers from FFS were selected intentionally (Table 1). The DD model also eliminated the in-built, systematic or seasonal effects other than the training effect.

Pharmacy and Therapeutics Committee
The Pharmacy and Therapeutics (P&T) Committee is a vital component of healthcare institutions, particularly in hospitals. It serves as a formal body that oversees medication management processes, ensuring that drug therapy is safe, effective, and cost-efficient.
Objectives of the Pharmacy and Therapeutics Committee
1. Formulary Management

• Definition: The formulary is a continually updated list of medications approved for use within a healthcare institution. It serves as a guide for prescribers regarding which medications are available and recommended for various conditions.
• Objective: The primary objective of the P&T Committee in formulary management is to ensure that the formulary reflects the most effective and safest therapeutic options based on clinical evidence. This involves evaluating new drugs for inclusion and removing outdated or less effective medications.
2. Drug Selection
• Rationale: The committee aims to specify preferred drugs and alternatives based on clinical guidelines and evidence-based practices. This helps minimize therapeutic redundancies and ensures that healthcare providers have access to optimal treatment options.
• Process: The selection process involves reviewing clinical studies, safety data, cost-effectiveness analyses, and recommendations from professional organizations to make informed decisions about drug inclusion.
3. Quality Assurance
• Importance: Quality assurance is critical for patient safety. The P&T Committee monitors medication use within the hospital to identify trends in prescribing patterns and potential safety issues.
• Monitoring Activities: This includes reviewing adverse drug reactions (ADRs), medication errors, and compliance with formulary guidelines. The committee implements corrective actions when necessary to improve safety outcomes.

Composting means decomposing organic wastes. It is a safe, non-polluting and microbiological method for disposal and recycle of organic wastes by converting those to balanced plant food materials, commonly known as compost, the organic fertilizer. In other words, the mineralization and partial humifi cation of organic materials by the action of microorganisms under suitable condition is called composting (Figure 6.1). The organic wastes undergo different physical and chemical changes with the help of microbes like bacteria, fungi, actinomycetes, etc. During composting the population of pathogenic microorganism reduces while that of benefi cial microorganism increases.

A system is a unit which possess a group of interacting components, operating together for a common purpose, capable of reacting as a whole to external stimuli. It is unaffected directly by its own outputs and has a specified boundary based on the inclusion of all significant feedbacks. For example, the human body is a system-it has a boundary (skin) enclosing a number of components (heart, lungs) that interact (the heart pumps blood to the lungs) for a common purpose (to maintain and operating the living body).

Ecosystem: Any collection of organisms that interact or have the potential to interact along with the physical environment in which they live form an ecological system or ecosystem. Ecosystems are not static entities but, they are dynamic systems with characteristic pattern of energy flow, nutrient cycling and structural change.

Agro ecosystem: Agro ecosystems are ecological systems modified by human beings to produce food, fibre or other agricultural products. Like the ecological systems, agro ecosystems are structurally and dynamically complex. But their complexity arises from the interaction between socio economic and ecological processes.

Crop system: An arrangement of crop population that transform solar energy, nutrients, water and other inputs into useful biomass that produce food, feed, fuel and fibre. Crop system comprise of soils, crop, weed, pathogen and insect subsystems. The crop can be of different species and variety, but they only constitute one crop system if they are managed as a single unit.

Cropping systems: It is defined as the order in which the crops are cultivated on a piece of land over a fixed period of time. Cropping system is the way in which different crops are grown. In the cropping systems, sometimes a number of crops are grown together or they are grown separately at shorter intervals in the same field.

Agricultural professionals continuously confront challenges in preventing pest infestations and managing temperature and moisture levels to protect grains from decay. They recognize the critical importance of safeguarding these valuable resources and rely on quick, dependable solutions to tackle common grain storage issues. Let’s take a closer look at the challenges faced by growers, handlers, transporters, and processors in long-term grain storage, and the strategies they employ to overcome these obstacles.
ABIOTIC FACTORS
There are also abiotic factors, which are non-living elements, that influence the quality of stored grains. Factors such as air, humidity, and temperature play a significant role in grain storage. If these factors are imbalanced in terms of quantity or quality, they can have a detrimental effect on the stored grains.
SPACE CONSTRAINTS
As crop yields continue to rise, farmers face the challenge of finding enough storage space to accommodate the increased harvest. Often, last year’s grain still fills storage bins when the new crop arrives, prompting the agricultural industry to seek innovative storage solutions.
The most straightforward solution to a shortage of storage space is to build additional bins. However, this option is not always feasible for smaller farms or processing facilities with limited budgets. As a result, many choose to repurpose existing barns or store surplus grain outdoors in piles. Unfortunately, this outdoor storage method comes with its own set of challenges, including faster spoilage and pest issues. To counter these problems, some facilities use temporary storage options such as grain bagging systems or outdoor storage tents. Mechanical ventilation systems are also employed to ensure proper aeration, reducing the risk of spoilage before the grain is moved to its final destination.

Fungi

Definition

Fungi are eukaryotic, spore bearing, achlorophyllus, heterotrophic and thallophytic plants with varied forms and habitats, representing heterothallic or homothallic types of sexuality with characteristic sexual and /or asexual means of reproduction without tissue differentiation.

All animals have a range of ambient environmental temperatures termed as thermo-neutral zone. This is the range of temperatures that are conducive to health, performance and well-being. The upper critical temperature is the point at which heat stress begins to affect the animal adversely. There are a number of climatic factors like temperature, humidity, wind speed and radiant energy/sunlight that influence the degree of heat stress. Heat is also produced by metabolism within the body, which includes maintenance, growth and production. Heat production is affected by body weight, species and breed, level of production, level of feed intake, feed quality and, to a lesser extent, by the amount of activity and exercise. Excluding temperature of the air ventilating the house, heat is also added from the roof and walls. Heat stress can be defined as the point where the animal cannot dissipate adequate quantity of heat to maintain body thermal balance. During the periods of heat stress, most of the productive energy is diverted to thermoregulatory adaptations that results into decreased weight gain, poor immunity, oxidative stress predisposing animals to various infectious diseases and high mortality rates. In order to characterize and quantify thermal comfort zones according to animal species, several thermal comfort indices or heat stress indices were developed.

The primary focus for livestock and poultry has been on heat stress indices to provide guidelines for environmental management and risk assessment for losses through linkages with physiological responses (Eigenberg et al., 2005) or performance (Baccari, 2001). The linkage is based on observations of a selected performance criterion (e.g., growth, milk or egg production) made concurrently with measures of the warm environment. These serve as inputs for the selected heat stress index and thus an empirical relationship can be developed which is termed as ?biological response function?. These indices recognize the ability of the animal to cope with environmental stressors by adjusting physiologically, behaviourally and immunologically to minimize adverse effects or even compensate for reduced growth and production performance during moderate environmental challenges. When the intensity and duration of potential stressors exceed thresholds, coupled with limited opportunity for recovery, animals are unable to cope and therefore are affected adversely. Nevertheless, the heat stress indices have served as useful surrogates for the complex interactions between the physical and biological components (Hahn et al., 2003).

Studying livelihood systems requires a multidimensional approach, as livelihoods are shaped by a variety of social, economic, environmental, and institutional factors. To understand how individuals and communities sustain
their lives, researchers and policymakers use a range of indicators that help analyze both the opportunities available and the vulnerabilities faced by people. These indicators provide insights into the resources people use, the strategies they adopt, and the outcomes they achieve in their pursuit of a secure and dignified living. One of the foundational frameworks for analyzing livelihoods is the Sustainable Livelihoods Framework (SLF), which identifies five core categories of capital or assets: human capital, natural capital, financial capital, physical capital, and social capital. Indicators related to each of these asset types are commonly used in livelihood studies.
Apart from these core assets, livelihood outcome indicators are also important. These include levels of food security, poverty status, employment stability, quality of life, savings and investment behavior, and ability to withstand shocks. These outcomes provide a picture of whether the livelihood strategies adopted are sustainable and equitable. Institutional and policy indicators are equally significant. These include access to government schemes, land rights, legal entitlements, effectiveness of rural development programs, and availability of extension services. The role of markets, financial institutions, and governance structures must also be considered when evaluating livelihood systems. In the context of vulnerability, indicators like frequency of crop failure, income variability, health risks, and exposure to natural disasters are used to assess how secure or fragile a livelihood is. Coping strategies, such as migration, asset liquidation, or diversification of income sources, are also analyzed.

Introduction
Liposomes as Drug Delivery Vehicles
Liposomes, first described by Alec Bangham in 1961, are self-assembled vesicular structures composed of one or more lipid bilayers surrounding an aqueous core(Nakhaei et al., 2021). These lipid bilayers typically consist of natural or synthetic phospholipids, which can spontaneously form closed vesicles in an aqueous environment due to their amphiphilic nature. The resulting liposomal structure resembles a miniature cell membrane, with hydrophilic heads facing outward towards the aqueous environment and hydrophobic tails facing inward, forming the bilayer structure(Nogueira, Gomes, Preto, & Cavaco-Paulo, 2015). Liposomes are important in the field of drug delivery due to their unique structural properties, which enable them to encapsulate a wide range of therapeutic agents, including hydrophilic, hydrophobic, and amphiphilic compounds. (Guimarães, Cavaco-Paulo, & Nogueira, 2021) The size of liposomes can vary from tens to hundreds of nanometers, making them suitable for both systemic and targeted drug delivery applications. Liposomes can be formulated to exhibit specific properties such as prolonged circulation time, enhanced stability and targeted delivery.(Simões, Moreira, Fonseca, Düzgünes, & De Lima, 2004) The versatility of liposomes is due to their ability to encapsulate drugs within the aqueous core, embed drugs within the lipid bilayers, or attach drugs onto the liposomal surface through chemical conjugation or targeting ligands. This flexibility allows for the development of multifunctional liposomal formulations capable of encapsulating multiple drugs which facilitate combination therapy and theranostic applications.(Cheng et al., 2021; Eloy et al., 2014) Liposomes also offer several advantages over conventional drug delivery systems, including biocompatibility, biodegradability, and low immunogenicity. These characteristics minimize the risk of adverse reactions and systemic toxicity which makes the liposomes suitable for repeated dosing and long-term therapeutic applications.(Al-Jamal & Kostarelos, 2011; Alavi, Karimi, & Safaei, 2017; Goyal et al., 2005) However, liposomal drug delivery systems are also associated with several challenges which include issues related to stability, scalability, and manufacturing consistency.(Kraft, Freeling, Wang, & Ho, 2014)

1. Introduction

Among three major functions identification, classification and nomenclature of taxonomy (Fig. 1), identification is a core activity and one of the major objectives of taxonomy, which determines whether other two elements are the same or different. Classification and nomenclature are two separate methods in taxonomy, however, both are totally dependent on the correct identification of the objects. The identification of an unidentified plant with a correctly identified specimen also involves classification. So, both identification and classification, involve comparison and judgment of similarities and differences. Therefore, the identification is a primary function in classification involving nomenclature which performs an essential role as a means of communication. According to Blackwelder (1967) “identification enables us to retrieve the appropriate facts from the system (classification) to be associated with some specimen at hand” and is “better described as the recovery side of taxonomy.” The identification of plants is very important for proper assessment and characterization of biodiversity on which the whole life of human beings is dependent starting from birth to death. The use of biodiversity for various purposes like food, nutrition, pharmacognosy etc. requires accurate and reliable identification and naming of plants (Nesbitt et al. 2010).

Poultry rearing systems vary in scale and complexity, each suited to different production needs and environments. This chapter explores the main poultry housing systems, detailing their advantages and disadvantages to help in selecting the most appropriate method for various farming scenarios.

Housing Systems of Poultry

Generally, there are four types of housing systems in poultry, they are

1. Free range or extensive system,

2. Semi-intensive system,

3. Folding unit system and

4. Intensive system.

1. The plaster-bandage wrap cast :(Negative cast)

The amputee was restrained on lateral recumbency keeping the amputed leg upwards.

A thin, moistened stockinet was pulled over the stump and it was maintained snugly in place by tying with a string at the top.

The moistened cast stockinet was marked with indeliable pencil indicating prominences with a cross and other sensitive areas with a circle (Fig.1).

Plaster bandages were then wrapped as pen requirement unit I the shell has a thickness of approximately 3mm in proximal third.

After plaster hardened, it was removed either by pulling out or longitudinal splitting with a cutter (Fig.2).

All over the world, garden designing has a historical back ground. Every continent has a definite style based on cultural heritage, agro-climate and the rulers. There are influences of one style over the other and subsequent change in gardening style also. However, there are some recognized orthodox styles like ‘English Garden’, ‘French Garden’, ‘Japanese Garden’, ‘Italian Garden’, ‘Dutch Garden’, ‘Mughal Garden’ etc. These are unique and true to their specific characters. Modern gardens all over the world have been greatly influenced by all these styles.

History and characteristics of these gardens are given below in order to make one acquainted with these styles.

Dutch Garden

Initially, gardening in The Netherlands was started in the form of abbey and monastery gardens (middle ages 500-1500 A.D.). Design of the medieval gardens usually was elongated rectangle, sometimes square, also divided with cross paths. These gardens were mainly meant for production of vegetables, medicinal herbs and fruits besides flowers.

During the Dutch renaissance (1500-1650 A.D.) further gardening activities initiated. After independence in 1609, it gained momentum and the change of garden style also noticed. Gardens became more open without walls. However, the basic design was on the basis of geometrical patterns - rectangular divided into small separate square. Axis of the garden was not in relation to the building. Leafy walk path (berceaux) was main structural elements created between various sub-divisions. Bulbous plants specially tulip was most preferred in the garden besides marigold, carnation, poppy, rose etc.

Introduction Fruits and vegetables are basic foods for human physical health since they have a wide range of flavours and are linked to a higher quality of life. Horticultural crops are known to reduce the risk of a variety of chronic diseases. Recognizing the importance of fruits and vegetables in the human diet, demand for these horticulture food products has skyrocketed. The demand for fruits and vegetables has expanded significantly as a result of greater consumer awareness of dietary habits, the benefits of eating fruits and vegetables, and an increase in the global population. According to research by the Food and Agriculture Organization (FAO), horticulture product production for 2019 is expected to be 314.5 million tonnes, which is roughly 1% more than the global fruit and vegetable (F&V) production in 2017–2018. (FAOSTAT-FAO statistical database, 2019). Over 67 % of the world’s horticulture produce is produced in China, India, the United States, Turkey, Russia, Vietnam, and Mexico (Paggiola et al., 2016).

For the accurate identification and diagnosis of plant disease and plant problems a foundational knowledge of terms and definitions is vital for developing concepts, doing research, discussing and communicating issues and providing clarity to your work. The following terms and definitions are basic to the study of plant pathology. They are, however, just a brief introduction to the vocabulary of the science. If you have limited or no background in the subject and you are just getting started, the concepts and terminology of plant problems can seem somewhat daunting. However, your vocabulary and skill will develop through exposure to diagnostics, experience and correct use of the appropriate terms.
Plant pathology (gr., path -“suffering”- “logy”, the science of) is the study of plant diseases and the abnormal conditions that constitute plant disorders. Etiology is the determination and study of the cause of disease. A pathogen can be living or non-living, but usually refers to a live agent. A pathogen is an organism which causes a disease. Pathological is a condition of being diseased. Pathogenic is having the characteristics of a pathogen and pathogenicity is the capability of a pathogen to cause a disease.

Advertising is a crucial component of marketing, designed to promote products, services, or brands to target audiences. A well-planned advertising strategy enhances brand visibility, attracts potential customers, and drives sales. Various advertising strategies exist, each tailored to achieve specific business objectives and cater to different market segments. This chapter explores the different types of advertising strategies used by businesses to maximize their reach and impact. 1. Print Advertising: Print advertising involves placing ads in newspapers, magazines, brochures, flyers, and billboards. It is a traditional form of advertising that continues to be effective for local and niche markets. Advantages • Tangible and long-lasting: Print materials can be kept for future reference. • Targets a specific readership: Newspapers and magazines cater to specific demographics. • Builds brand credibility: E

Advertising is a crucial component of marketing, designed to promote products, services, or brands to target audiences. A well-planned advertising strategy enhances brand visibility, attracts potential customers, and drives sales. Various advertising strategies exist, each tailored to achieve specific business objectives and cater to different market segments. This chapter explores the different types of advertising strategies used by businesses to maximize their reach and impact.
1. Print Advertising: Print advertising involves placing ads in newspapers, magazines, brochures, flyers, and billboards. It is a traditional form of advertising that continues to be effective for local and niche markets.
Advantages
• Tangible and long-lasting: Print materials can be kept for future reference.
• Targets a specific readership: Newspapers and magazines cater to specific demographics.
• Builds brand credibility: Established publications lend trustworthiness to ads.
Disadvantages
• Declining readership: With digital media gaining traction, fewer people rely on print.
• Expensive for premium placements: High visibility ads cost significantly more.
• Limited tracking capabilities: Hard to measure direct impact and ROI.

Advertising is a crucial component of marketing, designed to promote products, services, or brands to target audiences. A well-planned advertising strategy enhances brand visibility, attracts potential customers, and drives sales. Various advertising strategies exist, each tailored to achieve specific business objectives and cater to different market segments. This chapter explores the different types of advertising strategies used by businesses to maximize their reach and impact.
1. Print Advertising: Print advertising involves placing ads in newspapers, magazines, brochures, flyers, and billboards. It is a traditional form of advertising that continues to be effective for local and niche markets.
Advantages
• Tangible and long-lasting: Print materials can be kept for future reference.
• Targets a specific readership: Newspapers and magazines cater to specific demographics.
• Builds brand credibility: Established publications lend trustworthiness to ads.
Disadvantages
• Declining readership: With digital media gaining traction, fewer people rely on print.
• Expensive for premium placements: High visibility ads cost significantly more.
• Limited tracking capabilities: Hard to measure direct impact and ROI.
2. Broadcast Advertising: Broadcast advertising includes television and radio commercials. It is one of the most powerful advertising methods due to its broad reach and audio-visual appeal.
Advantages
• High engagement with audiences: TV and radio captivate viewers and listeners.

3a. Sources and problems of agricultural wastes
3b. Waste Reduction
3c. Waste Treatment
3d. Waste Disposal
3e. Clean Technology

Different Kinds of Agricultural Wastes
Table 1: Common Hazardous Wastes: Community Source

Introduction

In agricultural extension and education, video production plays a pivotal role in disseminating information, training farmers, and promoting best practices. The effectiveness of these videos is often determined by the quality of their scripts. This chapter explores three primary types of video scripts: basic production scripts, storyboard scripts, and camera scripts. Each type fulfills a distinct role in the production process, and understanding their unique functions is crucial for creating engaging and informative content. By examining these script types, this chapter aims to provide a comprehensive guide to the scripting process, helping to enhance the quality and impact of educational and promotional videos.

Script

A script is the written content for visual stories, such as plays, television shows, or movies. While scripts often include stage directions and production notes for performers and crew, their primary focus is on the dialogue between characters. Scripts serve as a blueprint for the entire production, guiding actors and technical crew through their roles and responsibilities. For example, a script for a stage play will detail character interactions, emotional tone, and specific actions, ensuring that all elements of the performance align with the director’s vision.

4.1 Introduction

There are some mathematical tools that are applicable in solving the mathematical problems related to the subject matter on “Economic Theory”. Differential calculus is one of those mathematical tools. Hence the useful theory of differential calculus will be discussed in this chapter.

4.2 Differentiation

The mathematical term “Differentiation” is involved in finding the rate at which a variable quantity changes.

In the theory of “Differential Calculus” we are concerned fundamentally with the “Rate of Variation” in a function y = f(x) with respect to the change in other variable (Independent Variable X) on which the dependent variable ‘Y’ depends through a relation Y = f(x).

Integrating the function f (x) = 1 + e−x sin (4x) over the interval [0, 1]

using Simpson’s 1/3rd rule gives

(A) 1.021         (B) 0.091

(C) 1.321         (D) 2.642

Introduction

The phytohormone abascisic acid (ABA) was discovered in connection to studies related to shedding of fruits and leaves, also called abscission, and the dormancy of buds. It was first identified and characterized by Frederick Addicott and his associates in 1963 while studying the compounds responsible for the abscission of cotton fruits. Inhibition of growth and maintenance of the dormancy of buds are the most important visible effects of ABA. The concentration of ABA does not remain constant as its concentration decreases in the buds significantly after sprouting and rises during seed and fruit production. Its concentration in dormant seeds is very high and considered as an efficient inhibitor of germination. The role ABA plays during the abscission of fruits and leaves is not clearly understood as its effect is very clear in case of fruit abscission where as it is not very clear on the abscission of leaves. However, the regulatory effect of ABA on the water balance is established in plants. It also induces stomatal closure to inhibit further loss of water. Being a growth inhibitor, ABA also reverses the effect of growth-stimulating hormones like auxin, gibberellins and cytokinin in different tissues of a plant. One of the main goals of phytohormonal ecology is to study the interactions between biotic and abiotic stress at hierarchical levels of biological organization.