eBook Chapters

28.1. Aim

Estimation of reducing sugars in fresh fruits and processed products using Lane and Eynon method.

28.2. Theory

Sugars are inherently present in different fruits and vegetables or added during preparation of different products. They are added to the fruit products to improve the taste and also to act as a preservative. Glucose and fructose in the fruits represent reducing sugars while sucrose or cane sugar added represents the non-reducing sugar. They are estimated by using Lane and Eynon method which measures sugar as reducing sugar and total sugar as invert sugar.

32.1. Aim

To estimate the level of sulphur dioxide contents in fruit and vegetable products

32.2. Theory and principle

Sulphur dioxide in food product is added as class II preservative and may exists as undissociated sulphurous acid, as free bisulphate or sulphite ions or as combined SO2 in the form of hydroxy sulphonates. Sulphur dioxide is measured as free or total SO2. Estimation of SO2 in squashes, jam, RTS drinks is important as its presence beyond the prescribed FSSAI limits make the product not confirming to Indian Food Laws. Generally 2 parts of KMS (Potassium metabisulphite) releases one part of SO2 explained as under:

27.1. Aim

To estimate the titratable acidity of fresh and processed fruit and vegetable products.

27.2. Theory

Most of the fruit, vegetables and their products contain acid or mixture of acids. The acids may occur naturally in the fruit and vegetables or may be added during manufacture of different products or by lactic acid or acetic acid fermentation. Generally citric acid is added in most fruit products while in pickles, sauces and ketchup acetic acid is used. The acids are mainly responsible for the tartness or sour taste, thus estimation of acidity is used as the measure of tartness. They also help in preservation by lowering the pH of the finished products.

Principle
Total ash is the inorganic residue which remains after a feed stuff is ignited to carbon free at 550-600oC in a muffle furnace. In other words, ash is that part of the material which does not disappear at 550-600ºC, while all the organic matter (OM) gets burnet.

Requirement
    1.    Analytical balance
    2.     Asbestos sheet
    3.     Dessicator
    4.     Heater /gas flame burner
    5.     Metal tongs
    6.     Muffle furnace
    7.     Silica basin/crucibles

(Method of Saryre, 1963 and IAEA, 1969)
Introduction

This technique helps in detection of very small amount of stable elements in fairly large quantitites of extraneous matter. Trace elements like zinc, copper, molybdenum and arsenic which exist in minute amounts in certain organs or tissues. For example, to detect the amount of copper present in one gram of tissue. using 1 mg of thin copper foil as control. Normal copper contains 69 percent ⁶³Cu and 31 percent ⁶⁵Cu. The (n,r) reactions on these two stable isotope produce ⁶⁴Cu and ⁶⁶Cu respectively. The half life of ⁶⁴Cu is 12.8 h and that of ⁶⁶Cu is five minutes. Therefore the latter can be ignored, if more than that an hour elapses between the end of the irradiation and the measurement. The specific activity of the unknown separate is compared with that of the known standard separate, and the content of Cu in the original tissue sample is thereby calculated.

Object: Estimation of water potential of plant tissue by Chardakov’s method.

Material required: Test tubes, sucrose or polyethylene glycol (PEG) 6000, methylene blue and dropper.

Principle: Concentration of the solution in which a plant tissue is immersed changes. If the solution is hypertonic (with lesser yw) the tissue loses water and the solution becomes dilute. If the solution is hypotonic (with higher yW) the tissue absorbs water and the Solution becomes concentrated. If the solution is isotonic (with yw equal to the tissue sap) the tissue neither loses nor absorbs water and the solution concentration does not change. Thus the yw of this solution gives the yw of the tissue.

Introduction

Water is the chief factor in fish farming. Water carries oxygen, eliminate metabolic waste products, through its physical and chemical composition, determine the success of production. The major constraint is that the waste organic matter released into water.

Measurement of pH, temperature, conductivity, salinity, transparency, turbidity and solids are important parameters to play role in water quality. pH in water changes during the day and night in response to photo-synthesis which is supported by the sun light intensity. Temperature is a physical parameter which effected on fish growth as resulting of many bio-chemical processes. It changes with the time depending on air temperature surrounding site.

18.1 Estrus Detection
18.1.1 Estrus Detection on the Basis of External Signs

The following signs are suggestive estrous period in cattle and buffaloes

1.    Partial inappetance due to excitement

2.    Drop in milk yield

3.    Restless ness of varying degree

4.    Frequent micturition with raised tail and crouching the back and lumbar region

5.    Marked bellowing

6.    Swollen vulvar lips- Due to swelling the wrinkles on vulvar lips disappear. The vulvar lips appear turgid and stand prominently.

7.    Congestion of vulvar lips 

8.    A clear, shiny mucus discharge seen at the vulvar lips extending down to perineal region and smearing the hind quarters. The discharge is stingy, having good consistency and no odour.

Estrus is referred to as the period of estrous cycle during which the female animal allows the male for mating. It is the period of estrous cycle during which insemination is done to female animal so that the animal will become pregnant. During estrus, cow usually seeks out the male and ’stands’ for him to mate her. In all domestic species, Ovulation occurs during this phase of the cycle but in case of cow, ovulation occurs about 12 hours after the end of estrus period.

The word estuary is derived from the latin word ‘aestus’ meaning tide and the adjective ‘aestuarium’ means tidal. This means low ground covered by the sea at high water.

Apparatus and Reagents

    1.    Soxhlet extraction asembly. This consists of 3 parts (a) condenser at the top, (b) the soxhlet or extractor in the middle, and (c) the receiver flask at the bottom. The 3 parts are assembled by means of their grounds glass interchangeable joints (Fig. 1).

    2.    Extraction thimble.

    3.    Petroleum ether (B.P. 40ºC to 60ºC)

    4.    Constant temperatue bath or six heater hot plate with temperature controlling device.

All scientists working with laboratory animals must have a deep ethical consideration for the animals they are dealing with. From the ethical point of view it is important that such considerations are taken care at the individual level, at Institutional level and finally at the national level.

Individually each investigator has an obligation to abide by all the ethical guidelines laid down in this regard at institutional level. The Head of the institution, maintaining animals for scientific experiments , should constitute an animal ethics committee on animal experimentation to ensure that all experiments conducted on animals are rational , do not cause undue pain or suffering to the animals and only minimum number of animals are used. The constitution and term of reference of the animal ethic committee should be well defined.

An animal ethic committee should include: a senior biological scientist of the institute, two scientists from different biological disciplines, a veterinarian involved in care of animals, the scientist in charge of animal facility, a scientist from outside the institute & a nonscientific socially aware member. A specialist may be co-opted while reviewing special projects using hazardous agents such as radioactive substance and deadly micro-organisms etc. The investigator may be called in for any clarification if required.

The ethics committee has to examine all projects involving use of animals before implementation, to ensure that minimum number of animals is used in the project and the ethical guidelines are strictly adhered to. It will also examine that the scientists and technicians handling animals possess adequate skill to perform the experiment. All animals should be maintained under standard living conditions and experiments will be conducted under proper anesthesia and termination of an experiment, the animal will be humanely sacrificed under anesthesia. Before disposal it must be ensured that the animal is clinically dead.

An ancient Chinese proverb says

If you are thinking one year ahead, plant rice
If you are thinking ten years ahead, plant trees
If you are thinking hundred years ahead, educate the people

Subramanya Bharati, a great Tamil poet, wrote decades ago that nutrition and education were the two legs of a human being — nutrition for the body and education for the mind. We must, therefore, foster a movement for integrating academic excellence and social relevance in the curricula of our educational institutions. Our universities should promote the growth of the science of ecotechnology, which is the product of the integration of traditional knowledge with frontier science. This will need a mind-set change in relation to the knowledge and wisdom of our tribal and rural families.

In the 1990s, I developed the Iwokrama Rain Forest Programme, which represents the world’s largest adventure in the sustainable management of rainforests. The local Amerindian population welcomed me to Iwokrama with a song, which translates thus:

Introduction

The term 'research ethics' in social science research refers to human values, norms, and society approved arrangements that regulate scientific understanding go for society. It consists of the set of values, standards, and principles that serve as benchmark to determine appropriate and acceptable conduct tall stage soft he research process. It is the implementation of ethical practices that specifies norms and values as well as responsibility towards the academic community and society as a whole. 

Code of Ethics was observed by International Commission on Zoological Nomenclature for renaming of homonyms. The commission had issues declarations or opinions from time to time, which together constitute the start of a code of ethics in the field of nomenclature. The first Declaration of the international Commission was as follows:

• When it is noticed by any zoologist that the generic name or specific name published by any living author as new is in reality a homonym, and therefore unavailable under articles 34 and 36 of the Rules of Nomenclature, the action is for said person to notify the said author of the facts, and give said author an opportunity to propose a substitute name.

• When the author of a newly discovered generic or specific homonym is dead, the discoverer is free to rename the genus or species as he likes. It is common practice to rename the category after the author of the homonym. However, this practice was never universal and it is not a matter of ethics.

Indigenous culture

Indigenous means originating where it is found. If knowledge originates from, and is bound to local experiences, taking its local world not perhaps as the only one, but as the most relevant of all they know may be called indigenous. Indigenous knowledge is human lifeexperience in a distinct natural and cultural amalgamation within a unique local and timely setting. It is an authentic appropriation of being, meaning that this very process happens exclusively in a given locality at a given time. In the field of medicine, Chinese culture (indigenous) developed Acupuncture technique. An acupunturist places needles into the skin to balance out the flow of chi which is believed to be the energy that flows through the human body. In theory, Chi runs along 14 pathways called meridains and when a patient is injured, stressed, or ill, their chi is supposedly being blocked. By placing needles in the Meridians the patients chi is thought to be put back into balance. Like Chinese culture, indigenous culture of different places/countries occurs and have their own belief and methods.

Indigenous cultures speak in various ways, for example we could speak from the perspective of our so-called ‘civilizations’ but how do we know that this perception is not contrary to the truth. We could speak on the basis of our traditional religious concepts but again these concepts may in reality be the opposite of what we believe. If we speak about the indigenous from the point of view of an anthropologist we arrive at a cold and empty language, a study that only pays attention to external and superficial matters, in the same way an anatomist analyses our inner organs or our bones but they are still a long way from knowing the reality. We believe that it is better to speak about indigenous cultures on the basis of their own reality, in relation to how they were as much as how they are now because in the course of time from yesterday to today many things have happened that are worth examining.

Introduction

In India about 8.6 per cent of population enlisted in the category as ‘Scheduled Tribes’ under Article 342 of the Constitution of India, but they constitute almost 16 per cent of the poor. Lakshadweep is the most populous state with respect to proportion of tribal to total population (94.8 per cent) whereas, in Uttar Pradesh tribal population is lowest with only 0.6 per cent of the total. In Odisha tribal constitutes 22.8 per cent of total population. In India literacy rate of tribal is 59 per cent as against that of general category is 69 per cent. Tribal people are observed to be strongly associated with the forests, hills and remote areas, practising a unique life style, having a unique set of cultural and religious beliefs. Over the generations these communities have evolved time tested farming systems, which are purely subsistence in approach and are keeping pace in perfect harmony with nature. Over the recent few decades, however, population growth and lack of harmony between different agriculture and developmental policies have threatened these traditional agricultural systems for extinction. The encroachment of local Governments and private interests on forest and mineral resources has pushed the tribal groups on to increasingly degraded land, thus their survival became a constant struggle.

The primitive societies have passed through several stages of economic development everywhere in the world. Thus one can find the stages of food gathering, hunting and fishing, farming, etc. among the Indian tribes. The tribes living in the forests and hills usually earn their livelihood by means of food gathering, hunting and fishing. Such is the life of Kadar of Kerala, Birhor and Kharia of Bihar and other tribes. The tribes living in dense forests, full of wild beasts, live on hunting, such as the tribes of Naga, Kuki, Bhil, Santhal and Gond. The hunters leave the females to carry out household activities in the morning and return in the evening after hunting. In some tribes there is a usual custom of hunting collectively. The Nagas use spears, arrows and bows. The Bhils are very much specialized in shooting by arrow. The tribes living near rivers and seas usually earn their livelihood by catching fish. The hilly tribes rear the cattle, an example of which are Goojars and the tribes of Chamba. The Todas of Nilgiri rear buffaloes. Some tribes also carry out cultivation, but they are generally shifting from one place to another. So shifting cultivation is an intrinsic part of tribal system and tradition. Among the cultivating tribes are the Santhals and Gonds. Tribal groups mostly do not have land ownership and have a limited option to adopt agricultural operations in forest areas. Considering their sustenance Government of Odisha has passed an Act “Schedule Tribes and other forest dwellers Forest Right Act” in 2006. It permits ownership of tribals on forest land up to 4 ha. for forest use and subsistence agricultural activity without any penalty. Cottage industries, such as weaving cloths, preparing ropes and skins and utensils of different metals are prevalent in many tribes. The Kharia people are very much specialized to cottage industries. Their living standard is very poor in spite of their hard work.

Abstract

Lichenized fungi or lichens represent the most successful symbiotic organisms on earth. They are the inconspicuous group of lower plants having numerous properties. Their different growth forms namely crustose, foliose and fruticose have been put to use in one way or the other. Since ancient times, they were used as food, fodder, dye and in perfumery. Lichens are a rich source of several important secondary metabolites because of which they are widely used as a source of medicine against several diseases and to improve the health of humans as well as animals. Most of the information related to the usage of lichens is gathered from the traditional knowledge holders. Therefore, there is a need to explore and document this traditional knowledge associated with lichens. The present paper thus makes an attempt to present and document the valued information on the ethnobiological uses of lichens from the Northwestern parts of the Himalayas, if not, the undocumented information will be lost for all the future generations. Ethnobiology of 39 species of lichens belonging to 08 families is being presented in this communication.

Abstract

Crocus sativus L. commonly called as saffron is a Persian plant belonging to the Iridaceae family and is cultivated for more than 4000 years. It grows exclusively in India, the Balkans and the eastern Mediterranean region. Saffron is traditionally used for, as a food supplement and its pharmacological properties such as antihypertensive, anticonvulsant, anxiolytic aphrodisiac, antioxidant, antidepressant etc. It comprises of the dried stigma which comprises of 150 different types of metabolites, of which some are responsible for the color, flavor and aroma of the Saffron. Saffron is a plant with great significance as an adaptogen and seems to be promising as a nutraceutical in barring several diseases. The current chapter explores the Ethnobiology, historical background, chemical constituents, ethnopharmacological properties, uses and toxicity of Saffron.

Abstract

Plants have been the source of food and medicine since time immemorial. Tribal people have been using several plants and their parts as source of alternative food that is conventionally not consumed by urban population. However recently, due to increasing population and reducing agriculture land, such alternative foods are gaining attention. Such alternative foods often also have medicinal value and are consumed by tribals for ameliorating certain disorders. Due to rising costs of modern medicine and associated side effects, general public perception is positive for alternative medicines and traditional therapies. Yams are an example of such alternative medicinal foods and are the focus of this chapter. Here we describe the ethnobotanical and nutraceutical potential of yams. We have discussed, in detail, some of the species that are consumed in various regions of India. During the compilation of this data, it was noticed that detailed molecular information is not available for most species, while for some species almost no molecular data exists. Further, many species have not been properly evaluated through modern natural product chemistry and pharmacological screening methods to validate the claims made in ethnobotanical surveys. These and other lacunas in knowledge pertaining to yams have also been pointed out towards the end of the chapter.

Introduction

During the last few decades, interest in Ethnobotany has evolved substantially world wide, particularly in India, Africa, Mexico, South America, and South-East Asia. Ethnobiological information on what and how local people make use of plants and animals have been traditionally gathered and passed on from generation to generation. India represents one of the greatest emporia of ethnobotanical wealth. Like other parts of India, in hot arid region also, rural and tribal communities have precious information about utilization of indigenous plants. Besides identification of plants as food, fodder, medicine etc., there also exists similar experience on life support species for exigencies like drought and famines, which is less utilized so far. Several researchers made contribution on etnobotanical aspects of Thar Desert. During 19^th century, King (1869 and 1870) listed wild plants used as food and vegetables during famine by people of Marwar region of Thar Desert. Afterwards, ethnobotanical aspects of edible plants of this region was attempted by several workers

Cloud with dense forest and valleys the present Kalahandi district, Orissa comprises of ex–state of kalahandi and exzamindari of khariar minus Kasipur. The present district of Kalahandi district of Orissa state was a part of south Kasipur Kingdom. It continued as a princely state up to Nov. 1949 and then merged with orissa state the district Kalahandi was created out of exprinciply state of Kalahandi with addition of ex-zamindari areas of Khariar which formerly a part of Sambalpur district. Again Nuapada and Nawarangapur area separated from it and the present form of the district exist.

The modern Kalahandi district occupies the south western part of Orissa and is situated between 19 degree 10’ North Latitude and between 82 degree 30’ and 83 degree 50’ longitude. To the northern side of Kalahandi district Balangir, to south eastern side Rayagada district is situated. To south westen side Nawarangpur district, to the Southern side Koraput district and to north Nuapara district and western side Chhatisgarh are situated. The Kalahandi district extends over an area of 7920 sq. kms, which is 5.09% of total geographical area of Orissa and occupies 7th position in area of the state of Orissa. According to 2001 census, total population of the district is 13, 34,772 which has 3.64% of the whole state and density of population is 168 per sq. k.m. In the district, 2 subdivisions, 4 revenue Tahasils, 13 blocks, and 195 Gram Panchayats and 2205 villages are existing.

Minor millets, that belong to members of the cereal grass family, are advantageous in areas where large crops like Bread wheat, Rice paddy, and maize are relatively ineffective because they have a wide range of genetic diversity and thrive in marginal environments. The varied soil types, temperatures, heat and light cycles, and rainfall patterns across India all support the cultivation of minor millets. Finger millet, barnyard millet, foxtail millet, proso millet, and many more types of minor millet are used in agriculture. The majority of those who depend on these millets for sustenance and income are small-scale indigenous farmers and dwellers of rainfed areas, mainly among impoverished tribal populations numbering in the millions. Coarse cereals,now a days known as Nutri cereals or nutraceutical flora and are no longer known as coarse cereals as a potential global answer to malnutrition and unrecognized hunger. Minor millets have long been an essential component of Indian tribal societies’ agricultural systems and practices. They have used little millets for both famine prevention and ethnomedicine since the beginning of time. This chapter focuses on how little millet could help people have access to food and nutrition security while reducing hunger and malnutrition.

Abstract

Mentheae, one of the largest tribes of subfamily Nepetoideae of family Lamiaceae is represented by five genera (Mentha, Nepeta, Origanum, Salvia and Thymus) in Jammu and Udhampur districts (Jammu division). Frequent surveys made of these districts to generate ethnobotanical data revealed the existence of Mentha arvensis L., M. longifolia (L.) L., M. piperita L., M. spicata L., Nepeta cataria L., Origanum vulgare L., Salvia splendens Sellow ex Schult and Thymus serpyllum L. While the enlisted Mentha species are represented more or less equally in the two districts, N. cataria, O. vulgare, S. splendens and T. serpyllum are mainly adapted to temperate regions of Udhampur. The frequent interactions with the locals indicated that except M. spicata whose leaves are used for seasoning dishes or preparing refreshing drinks and those of O. vulgare which find utility in making herbal tea, others are largely used for therapeutic purposes. Amongst these species, maximum use value was noted for M. longifolia (0.81) followed by that for M. arvensis (0.69). So far as agreement among the informants regarding the usage of a species for curing a particular disease is concerned, it came out to be maximum for both M. longifolia and M. arvensis for treatment of respiratory (Fic=0.93) and gastrointestinal problems (Fic=0.93). In our study, most of the informants have predisposition towards using M. longifolia for treating respiratory disorders particularly cough and cold. For this species, fidelity level was found to be maximum equal to 33.33%. This indicates that M. longifolia carries great potential for using in new herbal formulations. Finding of lesser use value for O. vulgare (0.21) and T. serpyllum (0.30), reportedly having many medicinal important components, is a matter of concern. The probable reasons underlying quite less usage of these taxa have also been discussed in this communication along with the future prospects of cultivating these.

Abstract

Family Apiaceae (or Umbelliferae) is one of the largest plant group represented by 418 genera and 3257 species (TPL 2013). The plants members are mostly aromatic, used by local populace as traditional medicines as well as spice and condiments in their routine food preparations. They have characteristic aroma due to the presence of some essential oils or oleoresin. Members of this family possess large number of compounds having antibacterial, hepato-protective, vaso-relaxant, inducing apoptosis and antitumor activities. Although the family is distributed globally but Indian subcontinent is a major center of its diversification, where more than 200 species are distributed specifically in the Himalayan eco-terrains. Jammu province (Jammu and Kashmir, India) in North Western Himalaya having unique climatic conditions supports a large variety of medicinal and aromatic plants, and amongst these Apiaceae are well known. Local people uses these plants as vegetables, for culinary purposes and valued as traditional medicine like Bunium persicum (black cumin), Carum carvi (caraway), Coriandrum sativum (coriander), Cuminum cyminum (cumin), Foeniculum vulgare (fennel), Narthex asafoetida (Heeng), Trachyspermum ammi (ajwain) and Centella asiatica (Brahmi). People in Jammu province have their unique traditional way of using plants, knowledge of which is being transferred from generation to generation through oral dialect. As the data on the numbers of Apiaceae is scattered in the literature and difficult to be procured by the researchers. Therefore the current attempt is made so that the data along with some quantitative statistics can be provided at one platform only.

ABSTRACT

The present communication thr ows light on 118 species of flowering plants (Angiosperms) found in Dewghat forest (in Koraon forest range of Uttar Pradesh), which are traditionally used by the “Kol” tribal community and some other forest dwelling people, viz. Belbans, Bahelia, Mushahar etc. An enumeration of these plants materials is prepared with their botanical names, local / vernacular names, family names and using practices provided in this paper. This is the first ever report from the area under study on this aspect. In case of Anogeissus latifolia the leaf juice is used poisonous insect bite, grounded flower used in chronic diseases and its fruit is used asthma. The seeds of Cleome viscosa are given orally in stomach diseases. The leaves of Cassia alata used for ringworms and skin disease. The leaves juice and seed of Euphorbia thymifolia is used as a strong purgative. It leaf juice is also used for the remedy of ringworms and other skin diseases. It is an antidote for snakebite. The present study is aimed at indexing of all medicinal flora occurring in Dewghat forest (U.P.) and their uses for the benefit of the researchers to undertake studies on the prospects and potential of commercial exploitation.

Several ethnic communities inhabiting in the biodiversity-rich state of Meghalaya are primarily dependent upon the forest for their livelihood needs like food, vegetables, materials for construction of houses, firewood, medicine for curing numerous ailments and many other purposes. They have acquired vast knowledge about the wealth of plant and their utilisation. The present study was aimed to document such less-known wild edible plants used by Khasi people of the state. During the current investigation, a total of 94 species of such importance belonging to 80 genera under 47 families were documented. Among these, 46 are herbs followed by 29 shrubs, 12 trees and 02 are creepers. Based on the availability and commercial potential, 37 species have been sold in local markets for income generation. Of which 17 species are sold as fruit, 11 as leaves, six as root, rhizome and tuber, five species as a stem, two as inflorescence and one species sold as fronds. All the plants are arranged alphabetically in the tabular form, followed by families, vernacular name(s), plant part(s) used, habit (s), distribution and methodology. It has been observed that the knowledge of less known wild edible plants is significantly declining day by day. These plants are known for their importance in as food nutritional supplement and its medicinal value; if the information is not collected and documented, it may not last soon.

In the starting of twentieth century, the ethnobotany progressed from the untested compilation of knowledge to a bigger method and abstract reorientation. Nowadays, the ethnobotany practices need a spread of skills such as:

• Training on the identification and preservation of plant specimens
• Training on linguistic and sociology to understand local language and their family structure
• Training on the psychology and cultural or traditional about plants
• Training on pharmecology and medicobotany

Ethnobotanists interact in a very broad array of analysis queries and practices, that don’t lend themselves to straightforward categorization. However, the subsequent headings arrange to describe a number of the key areas of recent ethnobotanical study.

Abstract

Ladakh, a geographically isolated region, is known for its tough terrain and harsh climatic conditions. It remains cut off from rest of the country for 6-7 months in a year during winters. With limited food, fuel and other resources the people here have developed and sustained a long unique relationship with plants. They have been using both cultivated and wild ones as medicine, food, and fodder since centuries. The local populace possesses good knowledge of traditional methods of utilization of plant resources for diverse purposes, which they have acquired from their ancestors. However, this indigenous knowledge and age old practices are fast disappearing from the tribal communities, and there is an urgent need to save this treasure from getting irretrievably lost. Therefore, the present study is an attempt to document the various wild and cultivated plants along with their ethnobotanical uses as per the ancient practices of these tribal communities. For this purpose, an ethnobotanical survey was conducted in various villages of lower Ladakh. The investigation revealed a host of plants put to use by the natives in different indigenous ways. The scientific, family and local names of 50 species belonging to 48 genera and 31 families along with their uses have been enlisted in the present communication. As per the information gathered from the local inhabitants, these plants are categorized into five different groups. More than 50% of these are used as medicines, 38% are edible, 24% as fuel and 20% as fodder. Approximately 38% plants are employed in construction, as ornamentals, or sources of dyes, incense, cosmetics, etc.

Orissa is a land of tribes and one of India’s most important forests state providing home to a very large forest dependant tribal population. Represented by 22.5% of the total population of the states of tribes of orissa, which are scheduled as 62 different ethnic groups (Biswal et al., 1997). The orissan ethnographic scenario includes as many as 62 scheduled Tribes are enlisted in the presidential orders numbering 5,915,067 persons in 1981 census constituting 22.43% of the total population of the state (26,370 and 21). Distribution of tribal population in the district during 1981 census was uneven. Their number was more in inland districts like koraput, Mayurbhanj, Sundargarh, Keonjhar, Phulbani and Kalahandi than in the coastal plain consisting of Balasore, Cuttack, Puri and Ganjam (Mohanty and Chowdhury, 1990). Different tribes exists in orissa are namely divided into three groups on ethnolinguistic basic (i) Austro-Asiatic (ii) Dravidian and (iii) Indo-Aryan (Behura, 1993). The important communities are Saora, Bathudi, Bhumija, Bhuiyan, Saunti, Gond, Kharia, Kandha, Kisan, Kolha, Munda, Oraon, Paraja, Santal, Saora and Shabar (Behura, 1993). From different district of Orissa different ethno botanist worked on geographical basis or on a particular tribe, on a particular plant, food habit, and religious occasion, social customs etc. district wise enumeration is given below.

Jain, (1971) studied magico-religious beliefs pertained to plants prevalent among different tribals of the state. Rai chaudhuri et al., (1975) reported ethno botanical uses of 38 species from the state 81 plant species used by the rural folk for antifertility, as medicine, fibre and food were reported by (Saxsena and Dutta, 1975). Saxsena et al., (1981) gave an account of ethnobotany of 83 plant species.

Abstract

Asteraceae, one of the largest families of flowering plants, is of worldwide distribution and inhabits almost every environment except Antarctica. The family exhibits various life forms and is known to harbour a wide variety of phytochemicals. The study aimed at systematically documenting the precious ethnobotanical knowledge (ethnomedicinal, ethnotoxic and wild edible plants) of Asteraceae in district Udhampur. A total of 42 infomants between the age group 21-90 years were interviewed and 28 plant species of Asteraceae belonging to 26 genera were observed to be of ethnobotanical significance in district Udhampur. All the taxa were growing in wild and majority of them were herbs. The ethnomedicinal formulations were prepared in the form of paste, decoction, powder, etc which were found to be effective against plethora of ailments. The ethnotoxic plants were observed to exert their adverse effects by causing different ailments or diseases. The present study may serve as a base line data for further research and will go a long way in the treatment of major and minor ailments, protecting people from being poisoned by substances that may have cured them and also serving as food source.

Major Families

This chapeter deals with indigenous knowledge on use of various plant species in traditional medicine, food, dye, wood and in tanning etc.

Malvaceae

The family Malvaceae is also known as Mallow family, characterised by mainly shrubs and herbs. The plants of this family frequent have stellate trichomes, persistant sepals, epicalyx present, stamens numerous and monadelphous, anthers monothecous, carpels 5 or more.

Abroma angusta (Devil’s Cotton)

Paste of the leaf is applied to heal wounds and to relieve joint pain or back pain. Young shoots are used as food. Roots are used to cure itching.

Abutilon indicum (Kanghi)

The whole plant is used to make brooms. Plant also has medicinal value and its sap is used against ulcer, leprosy, piles, dysentery and fever.

Dioscorea L. commonly known as yam is the largest genus in the family Dioscoreaceae with 602 species distributed in the tropical and subtropical regions. It is an important food crop next to cereals and grains due to high yield storage of carbohydrates which form an important place in the dietary habits of small and marginal farmers especially in the food security of tribal population. The tubers are rich source of various secondary metabolites like Diosgenin, a commercially important bioactive sapogenin used as a precursor in the manufacturing of sex hormones, oral contraceptives and other pharmaceutically important steroidal drugs. Despite of its huge diversity only few species are recognized to be useful for medicinal and edible purposes. About 50 species of Dioscorea are distributed in India. The North East India, one of the hotspot of biodiversity is particularly rich in tropical root and tuber crops. Dioscorea species are in luxuriant growth in wild habitat in this region. The present study mainly addressed to explore the ethno medicinal uses, phytochemical analysis and also focused on safe conservation of sixteen species of Dioscorea found in this region.

1. Introduction

The North east India (located between 87Ú32’E to 97Ú52’E latitude and 21Ú34’N to 29Ú50’ N latitude) forms a distinct part of the Indo-Burma hotspot which rank the 6th among 25 biodiversity hotspot of the world is climatically, ecologically and ethnically very diverse. The region is largely dominated by tribals such as Naga, Khasi, Mizo, Miri, Adi, Aka, Apatani, Maripa, Mushard, Garo, Tagin Naga and Singhpho who are sociocultural different. The North east region, besides having large floral biodiversity, is yet not fully explored. The forest resources available in this area are most often ill managed due to lack of awareness and anthropogenic activities. Various wild roots and tuber crops are consumed by the indigenous group of North East India which have very less scientific documentation although. As wild tuber crops available in North East India, Dioscorea species play a prime role in providing food and medicinal requirements for the indigenous communities of this region. Hence the present paper focuses on the ethnobotanical importance and qualitative screening of few important phytochemicals present in the various species of Dioscorea, their conservation strategies and sustainable utilization in North East India.

Very few traditional systems have a fair level of documentations in place. Most of the little traditions, the flow of knowledge is mainly through word of mouth, however, the great traditions such as Ayurveda and Traditional Chinese Medicine do have a good level of documentation. For instance, Ayurveda means science of life in Sanskrit and aims at holistic management of health and disease. It remains one of the most ancient medical systems widely practiced in Indian subcontinent and has a sound philosophical, experiential and experimental basis541. Charak Samhita and Sushrut Samhita (100-500 B.C.) are main Ayurvedic classics, which describe of over 700 botanicals along with their classification, pharmacological and therapeutic properties542,543. A classic diagnostic text Madhav Nidana includes over 5000 signs and symptoms. During the course of evolution and years actual practice, the interpretation of such documents developed different schools of thoughts and resulted in wide variations in understandings. Similar situation also exists in other systems including TCM. These differences often raise issues related to authenticity and correctness of Traditional Knowledge (TK). Thus systematic documentation, interpretations and harmonization of concepts and practices remain a major challenge in most of the systems of TM. Many governments, professional and community organizations have undertaken such documentation and harmonization exercises. Projects like Traditional Medicine Knowledge Digital Library, AyuSoft, Triskandha Kosha and Medicinal Plants Database of FRLHT are attempting systematic documentation of Ayurveda in India544. Other databases such as The NAPRALERT (NAtural PRoducts ALERT) developed by Professor Norman Farnsworth contain bibliographic and factual data on natural products, including information on the pharmacology, biological activity, taxonomic distribution, ethno-medicine and chemistry of plant, microbial, and animal (including marine) extracts. Another project relates to the Global IP, Benefit Sharing and Traditional Medicine Database. It is being developed as a component of the Global Information Hub on Integrated Medicine from the work of the Commonwealth Working Group (CWG) on Traditional and Complementary Health Systems, now directed by the Malaysian Ministry of Health. This Information Hub is planning to create a network of legal centers, scholars and NGOs working together to develop a comprehensive legislative and policy review, information resource and exchange on issues pertaining to IP rights over traditional medical knowledge and the use of medicinal plants545. Other than such efforts, there is limited systematic documentation of TM knowledge and practices.

Abstract

Medicinal plants in recent years attracted much attention of researchers and become the basis of modern pharmaceuticals because of their active biologically compounds. Boerhavia diffusa commonly known as Punarnava belongs to family Nyctaginaceae is a widely distributed herb that has been naturalized in many areas of the world. It is utilized as a green leafy vegetable in many Asian and African countries besides possessing anticancer, antiestrogenic, immunomodulatory and antiamoebic activities due to the presence of valuable phytochemical compounds. Medicinal value of this plant is because of presence of specific kind of phytoconstituents like alkaloids, flavonoids, triterpenoids, lipids, steroids, lignins, proteins, boeravinone A-F, glycoproteins and carbohydrates which are present in huge quantity in this herb. In view of its therapeutic importance, it is widely used in Ayurvedic, Siddha, Homoeopathy, Unani, Modern and Tribal medicinal system. Whole plant sometime its parts are utilized for curing different disorders by tribal people in India. It is a well known ethnomedicinal plant species mostly found in subtropical and tropical regions of the world.

Abstract

Plants have been used both in the prevention and cure of various diseases throughout the centuries. An important prerequisite for proper utilization of plants as natural resources is that we should have full knowledge regarding their occurrence, frequency, distribution and phenology. Arisaema jacquemontii Blume (Araceae) is a perennial herb that has been reported in various literatures to be used traditionally in various countries for the treatment of different diseases. Scientific evaluations of this plant have revealed that it harbors various phytoconstituents like alkaloids, phenols, terpenoids, flavonoids, glycosides, tannins and several other chemical constituents which have pharmacological implications with as antioxidant, antifungal, antibacterial, anticancer and immunomodulatory activities. This chapter collaborates the data with respect to the ethnobotanical and ethno medicinal importance of Arisaema jacquemontii and its role as a natural source of phytoconstituents that have therapeutic implications.

Abstract

India with plethora of flora and fauna is a rich repository of cultural and ethnic diversity. Bestowed with varied biogeographically distributed ecosystems and biodiversity hotspots, the country has always been the torch bearer as far as ethnobotanical knowledge is concerned. Jammu and Kashmir in the North-Western part of India has a rich biological and cultural diversity. Owing to the distinct geographical location and varied climatic conditions, district Poonch represents a rich repository of biodiversity including the wealth of medicinal plants. This study aimed at documenting and examining the use of plants in various medicinal herbal preparations. A total of 223 plant species belonging to 77 families and 173 genera were documented to be used as ethnomedicine by people residing in the study area. The portion of plant parts used were leaves (28.14%), followed by root (16.33%), fruit (11.55%), whole plant (10.3%), stem (09.79%), flower (7.53%), seed (5.27% each), rhizome (4.77%), tuber (3.76%), bulb (1%) and bark and latex (0.75% each). These parts are utilized as herbal remedies for curing 109 human ailments such as abscess, acidity, asthma, back pain, body pain, boils, cancer, cholera, cold, cough, dandruff, erectile dysfunction, eye pain, fever, gastroenteritis, haemorrhoid, high blood pressure, menstrual disorders, sexual disorders, stomach pain, diabetes, diarrhoea, dysentery and weakness. The dominant disease was fever (52), followed by abdominal pain (30), cough (29), headache (22), constipation (20), diabetes (18), wounds (17), weakness (16) and others. There is need for proper documentation and an attempt has been made to highlight high value medicinal plants so that proper strategies could be made for their conservation.

Medicinal plants constitute a group of industrially important crops which are of great value for domestic use and for export. Plant-best drugs are being increasingly preferred in medical science. The use of various parts of several drug plants to cure specific ailments has been in vogue from ancient times on our indigenous medicine.

We are fortunate to have rich heritage in the form of information available from tribal areas gathered by the tribals through experience of generation in the form of folklore medicine. It is however unfortunate that full potential of these crops has not been scientifically explored. As compared to agricultural crops on which researches have been done for getting better varieties for high yields diseases resistance and better quality comparatively, little work has been done on the medicinal plants. The Ethnomedicinal plants are enumerated in subsequent pages and in Table 1 with their local name and families.

The review deals with 151 species of plants covering 138 genera and 66 families used by the tribes for curing women diseases involving 172 practices. Fabaceae is the dominant family with 13 species followed by Caesalpiniaceae (9 spp) and others curing a variety of ailments. The useful plants be subjected to phytochemical and pharmacological tests to determine the effective constituents and characteristic biological activity.

1. Introduction

Primitive man gets much enthusiasm towards green plants and started to examine the property of using plants by trial and error and obtained different beneficial properties. India is one of the 12 megadiversity countries and comprises of two hot spots. The World Health Organization (WHO) has compiled a list of 20,000 medicinal plants used in different parts of the globe. In India the scheduled tribe population is 104,545,716 constituting 8.6% of country total population of 1,210,726,932 (Census 2011) belonging to over 550 tribal communities and 227 ethnic groups. They inhabit about 5000 forested villages and occupying about 15% of country’s geographical area.

Abstract

Toxic plants are a grave threat to existence and bodily functions of humans and animals resulting in death or sickness through accident, unawareness or bad intention resulting in huge economic loss. They have always been part of our daily life and some of them are so prevalent that we do not even doubt their poisonous nature. The study aimed at systematically documenting the precious traditional knowledge regarding the ethnotoxic plants of district Udhampur. Ethnotoxic data was collected by interviewing 125 infomants between the age group 24-95 years. A total of 89 plant species belonging to 69 genera and 43 families were observed to be toxic to either humans or animals or both. Majority of the taxa were growing in wild. Leaves were observed to be the most toxic plant parts. Frequently encountered toxic plant families include Fabaceae, Solanaceae, Asteraceae, Euphorbiaceae, Apocynaceae and Ranunculaceae. The route /mode of entry of toxic chemicals was mainly observed to be ingestion. A total of 62 ailments/ conditions/diseases were reported to be caused in humans or animals or both by the ingestion, contact or inhalation of toxic plants in the present study. These include nausea, vomiting, headache, diarrhoea, hematochezia, abdominal spasms, anorexia, dysuria, deep yellow or no urine, bloat, etc. The present study may serve as a base line data for further research and will go a long way in safeguarding people from being poisoned by stuff that may have healed them.

Abstract

India harbors a wide variety of medicinal plants used traditionally in various remedies to treat livestock ailments. The therapeutic value of these plants attributes due to the concentration of several bioactive compounds present in them. Indigenous tribal communities are the treasurer of age-old traditional knowledge which inhabits Asian country since centuries. District Poonch exhibits a rich repository of traditional knowledge as major part of the population comprised of nomadic tribes such as Gujjars and Bakerwals which are still practicing the primitive traditional ways to treat diseases. In the present investigation, the traditional herbal plants and associated remedies for treatment of various veterinary disorders are presented. A total of 34 plant species belonging to 30 genera and 21 families have been reported which are being used by indigenous people for ethnoveterinary purposes. The majority of ethnoveterinary plants recorded from the district belong to families such as Ranunculaceae and Araceae (4 species each), Acoraceae, Amaryllidaceae, Apiaceae, Fagaceae, Lamiaceae, Primulaceae, Rutaceae (2 species each) and rest of the families members were represented by one species each. The frequently used plant parts are roots (30.23%), followed by tubers (18.6%), leaves (16.28%), fruits and whole plant (13.95% each), stems (4.65%) and seeds (2.33%). It has been observed that numerous formulations are made from these parts which are used for the treatment of various cattle infections such as abdominal colic, allergic infection, anestrous, chest diseases, constipation, cough, digestive troubles, FMD, fracture, galactagogue, gaseous bloat, induce puberty, internal injury, lung disease, pneumonia, quick discharge, removal of Leech, sexual stimulant, skin infection, snake-bite, stomachache and weakness with snake bite being dominant. The present study infers that the documentation of ethnoveterinary knowledge from the district requires utmost attention and may give leads or serves for further biochemical studies for the discovery of novel plant based drugs which will certainly helps in commercialization and betterment of tribals through benefit resource sharing.

Introduction

Harshberger first used the term ethnobotany in 1895-96, for the plants used by the local tribes and people, for their basic needs and requirements. They also used plant, plant parts and plant products for animal healthcare and other practices. This indigenous or traditional animal health care practice is called ethnoveterinary medicine. Ethnoveterinary medicine is defined as a holistic comprehension of the indigenous systems of animal health, their interpretation through western medicine and the development of effective and appropriate technologies (McCorkle 1986). McCorkle et al. (1989) define Ethnoveterinary medicine (EVM) as dealing with the folk beliefs, knowledge, skills, methods and practices pertaining to the health care of animals. McCorkle et al. (1996) give a description of ethnoveterinary medicinal research as the holistic interdisciplinary study of the local knowledge and the socio-cultural structures and environment associated with animal health care and husbandry.
 
EVM consists of local people’s knowledge pertaining to animal health and production. According to Mathias (1996 & 2002), it includes:

4.1. Fruit ripening

• Genetically programmed stage of development overlapping with senescence.

• Ripe when attains full flavour and aroma

• Optimum ripening condition for fruits• Conditions for controlled ripening of fruits at RH of 85-90%

5.1.1. Climacteric fruits

• Apple, apricot, avocado, banana, ber, cherimoya, fig, guava, kiwifruit, mango, melons, pear, peach, plum, persimmon, papaya, tomato, sapota and passion fruit

5.1.2. Non climacteric fruits

• Carambola, cherries, citrus, grape, litchi, loquat, olive, pineapple, pomegranate and strawberry

Difference between climacteric and non-climacteric fruits

Plant hormone ethylene is involved in a number of physiological activities within the plant system, ranging from seed germination to fruit development. Ethylene acts as a key player in accelerating fruit ripening by regulating the activities of different enzymes, and is responsible for shortening the shelf life and associated post-harvest losses during the storage of fruit crops. However, all fruit crops have different ethylene production capacities and vary in their response to ethylene. Hence, effective management of post-harvest ethylene is imperative for addressing the global challenge of reducing fruit losses after harvesting. In recent periods, numerous technologies and compounds have been developed for the suppression of ethylene and for enhancing in-situ oxidation of ethylene or inhibiting its production at a receptor level. Among these emerging techniques, the use of sachets, fumigating agents, catalysis and composite films embedded with nanoparticles has been found to be trending, but the use of some of compounds has been limited due to health risks or consumer unacceptance. However, most of these technologies are very costly and are not affordable for marginal farmers and developing nations. In order to address these issues, long term breeding approaches for shelf-life improvement, targeting the tfs, ERFs have been involved. This manuscript fills the knowledge gap by encompassing all the recent progress made in enhancing the shelf life of fruits by managing ethylene during storage.

ABSTRACT

Soft rot of ginger caused by the fungus Pythium aphanidermatum is a major disease affecting ginger cultivation. To combat invading pathogen in the event of infection plants are known to produce antimicrobial pathogenesis related proteins (PR proteins) prominent among which are antifungal hydrolases like glucanases and chitinases. Since Pythium lacks chitin in its cell wall, increase in level of glucanase in the plant may enhance the resistance of ginger to soft rot disease. Signal molecule like ethylene plays a prominent role in the defense response of plants. The present study was undertaken to investigate the role of ethylene in enhancing the level of glucanase in the ginger system. Detached leaves of Rio de Janeiro variety of ginger were used in different experiments. Leaves were treated with ethephon at different concentrations and different time intervals. Increase in the glucanase activity by ethephon treatment was quantified by enzyme assay using crude protein extract. Glucanase levels showed an initial increase with increasing concentration of ethephon but decreased at higher concentration (5 mg/ml). A longer exposure of detached leaves to ethephon (48 h) resulted in 3.7 fold increase in glucanase activity compared to control. The level of enhancement of the glucanase transcript also followed a similar trend as revealed by RT PCR analysis. Two acidic isoforms of glucanase were detected using native PAGE and their levels were found to be enhanced by treatment with ethephon. An increase in the levels of glucanase may contribute to the plant’s ability to resist infection by Pythium.

Ethylene (C₂H₄ also known as ethene) is a gaseous organic compound that is the simplest of the alkenes chemical structures (alkenes contain a carbon-carbon double bond). The hormone effects of ethylene on general plant growth were first noted in 1864 when leakage from gas street lighting systems caused stunting and deformation of nearby plants. In 1901 Neljubow identified the active component of the gas to be ethylene but it was not until 1934 that Gane identified that plants could synthesize ethylene and in 1935 Crocker proposed ethylene to be the hormone responsible for fruit ripening and senescence of vegetative tissues.

Ethylene play important role in many plant during development processes, including seed germination, vegetative growth, leaf abscission, flowering, senescence and fruit ripening. Ethylene also plays a role in response to water stress, chilling and mechanical injury.

Answer the followings
    Q.1.    What is the relative incidence of  Streptococcus agalactiae, other Streptococci and Staphylococcus aureus
    Ans.    1:1: 2
    Q.2.    What are sources of contagious pathogen for mastitis?
    Ans.    Udder surfaces of infected quarters 
    Q.3.    What are sources of environmental pathogen for mastitis?
    Ans.    Bedding of cow’s environment
    Q.4.    When animal get infection from environmental pathogen?
    Ans.    Animal get infection at any time during the life such as milking time, between milking, during dry period and prior to first calving.
    Q.8.    What is the probable period of new infection from environmental pathogen?
    Ans.    Early part of dry period and first two months of lactation.
    Q.9.    What is the relation of growth of Corynebacterium bovis in milk and lactation?

Etiology

Etiology is the study of cause of disease. It gives precise causal diagnosis of any disease. Broadly, the cause of diseases can be divided into two

a. Intrinsic causes.

b. Extrinsic causes.

Intrinsic Causes

Those causes which determine the type of disease present within an individual over which he has no control. These causes are further divided into following subgroups:

Genus

Specific diseases occur in a particular genus or species of animals. e.g. Hog cholera in pigs, Canine distemper in dogs

Introduction

The international fish trade increases day by day reaching to a tune of USD 151 billion in 2020 (FAO, 2022). Many developing countries like India gain benefits from this trade as income through net foreign exchange reached to a level of 8.09 billion USD in 2019-20. Seafood supplied by developing countries contribute to 77% of fish consumption worldwide. The European Union (EU) is the single largest market for fish and fishery products to a tune of 91.74 billion USD in 2022 as a consequence of an increased per capita consumption among its Member States. Spain, Italy, Germany and the United Kingdom are the largest importer of fish and fishery products.

To protect consumer health and to protect the territory from the introduction of animal and plant diseases, the European Community (EC) has laid down joint conditions for imports of foodstuffs. The Directorate General for Health and Consumer Protection (DG SANCO) is responsible for food safety in the EU. The import rules for fishery products are framed of high standards with respect to hygiene, consumer safety and animal health status. It is important that importing countries and food business operators (FBOs) must understand the fundamental principles and requirements of the European Food Law to ensure efficient and smooth imports. The EU system relies on Government to Government assurances without any intervention of any private type certification or standards. The Competent Authority (CA) in the country of origin is responsible for giving the official guarantees to the EU regarding compliance and conformity to all requirements of its legislation. The Export Inspection Agency (EIA) is the Competent Authority in India for issuing the required official guarantees for export of fish and fishery products. The EIA maintains a complete list of all establishments under its control.

12.1. Origin and distribution

• Native of Australia and Malaysia.

• Introduced by British people in Nilgiri hills of Tamil Nadu for fuel and timber.

• Commercially grown in Seychelles, Java, South Africa, Guatemala, Congo and Brazil.

• Eucalyptus tereticornis - Commercial species

• Major growing states - UP, Tamil Nadu, Kerala and Karnataka.

12.2. Economic importance

• Known as spotted gum tree.

• Source of citronellol, hydroxycitronellal and menthol.

• Contains 0.5-4% essential oil; rich in citronella.

• Essential oil - Therapeutic and ayurvedic benefits.

• Used in soaps, mouth wash liquids, massage oils, air fresheners and bug repellents