eChapters

Introduction

In spite of considerable progress achieved during the last three decades in horticulture production in India, the average productivity of different horticultural crops is quite low as compared to the best yields at national and world level. This indicates the scope to enhance the yield in different horticultural crops by many fold along with its quality specially in vegetables. This is because of an appealing gap between potential yield and the actual yield of various vegetable crops. For many vegetable crops, the yields realized are even less than 50% of their potential yield. Some time the farmers who are growing their horticultural crops during main season are even not getting back their cost of production, but the prices of the same horticulture produce is very high during off season. Several biotic and abiotic stresses are the major factors for low productivity and poor quality in several horticultural crops under their open field cultivation during rainy and post rainy season more specifically in vegetables. The increasing demand of off-season and high quality vegetables in various markets of the big cities has calls the attention of the vegetable growers for diversification from traditional way of vegetable cultivation or from traditional agriculture.

Introduction

The achievements in agriculture and its allied areas has made our country self sufficient economically. Our country has many landmarks in production of the grains, milk, meat, flower, vegetable etc which not only fulfill our own demands but even earning a lot of foreign exchange from export. Unemployment is a major problem effecting the growth of our country. The reasons for higher state of unemployment in rural areas can be attributed to poverty, law education and lack of job appointment. Alternatively, if the youths are self employed in the agrobased enterprises, then the problem of unemployment could be reduced to a certain limit, especially in the villages. There are so many vocations in which youth could be engaged such as dairy farming, poultry, piggery, beekeeping, mushroom cultivation, flower cultivation etc.

Introduction

Ever since the civilization of mankind, efforts have been directed towards accumulating and storing foods when they are in plenty in order to meet needs during the days of scarcity. In case of food grains not much problem was faced due to nature’s noble way of reducing the moisture level as the grains mature. However, in case of fruits and vegetables, long-term storage in their fresh form was not possible (until development of modern methods) primarily due to their high degree of perishability owing to high moisture content of these commodities at the time of harvest.

Appropriate Post Harvest Management (PHM) of horticultural crops fruits and vegetables in their fresh form prolongs their usefulness and in some cases improves their quality. It also checks market glut, helps in orderly marketing, increases financial gain to the producers and preserves quality of produce for much longer time. Horticultural produce like fruits, vegetables and cut flowers are living, respiring tissues separated from the parent plant. Therefore, the aim of PHM is to control various physiological processes viz. respiration, transpiration and other metabolic activities to keep produce in maximum usable form.

Introduction

In India Agriculture was practiced formerly on a subsistence basis; the villages were self sufficient, people exchanged their goods, and services within the village on a barter basis. With the improvement in rural infrastructure all these goods and services has become commercial in character, resulting in development of Micro-enterprises and requirement of their business promotion.
Businesses with five or fewer employees, called micro-enterprises, support many rural and urban households in developing nations. The term marketing connotes a series of activities involved in moving the goods from the point of production to the point of consumption. 
Marketing is an integral part of micro-enterprise products to encourage them to make more investment and to increase production. Thus there is an increasing awareness that it is not enough just to produce however it must be marketed as well.

Introduction

With the major thrust of extension agencies now focused on production techniques, the market-led extension holds the key to the future. This assumes greater significance in the light of the new international trading regime under WTO as the export opportunities are now being opened-up to India, which may have direct implications on agro-enterprise development.

In the light of the above, the extension functionaries in India are presently ill-equipped to deal with such challenges. It appears now that the production of the agri-produce is going to be dictated by market requirements. In this age of WTO, the extension methodology also needs to work out for dissemination of good agricultural practices to the farmers based on internationally accepted standards. This recommendation has now been made in the 05th National Extension Education Congress held in 2009 jointly conducted by Indian Council of Agricultural Research (ICAR) and CS Azad University of Agriculture and Technology, Kanpur (Proceedings of the 05th National Extension Education Congress, March 05-09, 2009, ICAR and CSAU, Kanpur). 

ABSTRACT

Agroforestry a sustainable land use system being practiced since long back through out the country. A diverse climatic, edaphic, and environmental conditions prevailing in the country has made it possible to practice different kinds of agroforestry systems. The local research organizations and individuals have undertook various studies for the betterment of the local agroforestry systems for getting higher yields to meet the need of food, fodder, fuel wood etc. It was found that Jowar+Teak+Papaya followed by Chilli+Teak+Papaya combination gave more economic returns compared to pure agricultural crop. Casuarina equisetifolia, Grevillea robusta, Dalbergia sissoo and Melia dubia have been found to be suitable for agroforestry systems in dryland. Coffee based agroforestry system and other tree-crop combination tried have been elaborated in this paper.

INTRODUCTION

Agroforestry is a new word for the old practice of growing woody plants with agricultural crops and/or livestock together on the same land. Various experts and scientists working on agroforestry or involved in land use related sciences have defined agroforestry according to their individual understanding of what they call agroforestry.

“Agroforestry is sustainable management system for land that increases total production, combines agricultural crops, tree crops and forest plants and or animals simultaneously or sequentially and applies management practices that are compatible with the cultural patterns of the local population” (Chundawat and Gautham, 1993).

A. Answer the following questions:

1.    What is proximate analysis?

Ans: Proximate analysisis a systemforapproximatingthenutritive valueof a feedstuff without actually usingit in a feedingtrial.

2.    Why proximate analysis is called Weende analysis?

Ans: In 1865, two scientists namely Wilhelm Henneberg and FriedrichStohmanndeviseda methodcalledWeende analysis/ proximate analysis at Weende Experiment Station, near the University of Goettingen, Germany. Hence, it is called ‘Weende Analysis’.

3.    Write the six proximate principles?

Ans: Water (moisture), ether extract (crude fat), crude fibre, total ash, crude protein and nitrogen free extract (NFE).

4.    Why ether extract is also called as crude fat?

Ans: As it is composed of true fats (glycerides of fattyacids) along with other substances like hydrocarbons, free fatty acids, fat solublevitamins,pigmentslike carotene & chlorophyll,waxes, sterols,lecithin(phospholipids), alkali substances,volatile oils and resins.

5.    What is NFE?

A. Answer the following question

1.    Writethe two assumptions whicharetaken into consideration during CP estimation of any feedstuff?

Ans: In the feed, a) all N is present as protein and b) All food protein contains 16%nitrogen.

2.    What is protein equivalent?

Ans: It is the arithmetic mean of the percentage of DCP and DTP (digestible true protein).

A. Answer the following questions

1.    What is adulteration?

Ans: It is an intentional admixture of a pure substance with some cheaper and low quality ingredients in order to make more profit.

2.    What is sampler?

Ans: The appliance used for the collection of samples is knownas sampler.

3.    What is dry rolling?

Ans: The process of disintegration of kernels into particles with the application of pressure by moving rollers is known as dry rolling or cracking.

4.    What is crimping?

Ans: The process of rolling of feed ingredients with the use of corrugated rollers is called crimping

5.    What is crumbles?

A. Answer the following question

1.    What is ANF?

Ans: The substances that are naturally present in the feed which by themselves or by their metabolic products interfere with the feed utilization (FCR), reduce production and affect the health of the animal are called anti-nutritional factors.

2.    How does lectin cause agglutination?

Ans: Lectins have the affinityfor certain sugar molecules. Hence, it combines with the glycoprotein components of RBC and there bycausing agglutination of the RBC.

3.    Why ruminants are more resistant to gossypol toxicity?

Ans: It is due to formationof stablecomplexeswith soluble protein in rumen which are resistant to enzymatic breakdown.

4.    Why ruminants of Hawaii and Indonesia are not susceptible to mimosine toxicity?

Ans: Ruminants of Hawaii and Indonesia possess ruminal bacteria which can rapidly degrade 3-hydroxy-4(1H)-pyridone (3,4 DHP) and thus are not susceptible to mimosine toxicity.

5.    How saponin form stable foam?

A. Answer the following?

1.    How antibiotic increases nutrient absorption from the gut?

Ans: In antibiotic fed animals, the intestine becomes thinner and healthier, thus they helps in more absorption of nutrient.

2.    What is probiotic?

Ans: Probioticcan be defined asthe live microbial food supplement that beneficially affects the host animal by improving the intestinalmicrobial balance.

3.    What is prebiotic?

Ans: These are the compound other than dietary nutrients that modifythe balance of microbial populationbypromoting the growth of beneficial bacteria and thereby maintain healthier intestinal environments.

4.    What is eubiotics?

Ans: Eubiotics are defined as non-antibiotic products maintaining the desiredbalance of good bacteriaand pathogens or eubiosis in the digestive tract. e.g. direct acting gut flora modulator (organic acid, essential oil), probiotics, prebiotics, immune modulator.

5.    What is supplement?

A. Answer the following questions

1.    Define feeding standards?

Ans: Feeding standards are the statements or quantitative descriptions of amounts of one or more nutrients needed by the animals or human.

2.    Limitation of feeding standard?

Ans: Palatability and physical nature of the ration are not included and does not included the environmental factors because environment may change the nutrient requirements.

3.    Write down the merits of ME system?

Ans: ME gives more correct result than DE or TDN. It is because energy losses in urine and GDP are corrected in ME system and ME is cheaper and easier to obtain than NE values.

4.    What is the purpose of following feeding standard?

A. Answer the following questions:

1.    What is NPN substances?

Ans: Feedstuffs which contain nitrogen in the form other than protein or peptides aretermed as non-protein nitrogen (NPN).

2.    What is liquid diet?

Ans: Liquid molasses containing 2-3% of uniformly mixed urea fortified with minerals andvitaminsis named as ‘liquiddiet’.

3.    Name few amides.

Ans. Urea (46.6% N) and Biuret (35%N)

4.    Name few amines:

Ans. Histamine from Histidine, Tryptamine from Tryptophan, Tyramine from Tyrosine, Betaine from Sugar beet pulp.

5.    Name few alkaloids.

A. Answer the following questions

1.    WhyvitaminAdeficiencyis far common in captive carnivores than free rangingwildlife?

Ans: Because they are fed exclusively on carcass meat without liver, cod liver oil or synthetic sources of vitamin A and moreover, thay can not convert ?-carotene to vitamin A.

2.    Why weekly off day feeding is practiced in tiger in all Indian zoos?

Ans: This ispracticed tostimulatetheintermittentfeedingbehavior of tiger in wild condition.

3.    Why metabolic bone diseases are very common in captive carnivores?

And: As the carcass meat provided in captivecondition is deficient in Ca and ratio of Ca: P (1:20) is very highly imbalanced.

4.    Write the extreme specializations in GItract seen in vampire bats?

Ans: Like blood sucking parasites, their GI tract is reduced to a tube since it feeds only on blood.

A. Multiple choices (you have to choose most appropriate answer among 4 alternatives)

1.    Which of the following bacteria in rumen is not cellulolytic

a. Bacteroides succinogenes

b. Ruminococcus flavefaciens

c. Ruminococcus albus

d. Succinivibrio dextrinosolvens

2.    Which of the following bacteria does not interact with anaerobic fungi

a. R. albus

b. R. flavifaciens

c. Butrivibrio fibrisolvens

d. Fibrobacter succinogenes

Livestock feeds have a variety of feed stuffs for feeding. Composition and characteristics differ even amongcloselyrelated feeds.Also, cost and availability of such feeds at a particular place, makes the farmer to choose for practical feeding. Therefore, it becomes imperative to learn about such classes. Feeds may generally be classified according to the amount of a specific nutrient they provide intheration. Theymayconvenientlybe divided intotwo general classes– roughages and concentrates. Roughages are bulky feeds containing more than 18 per cent crude fibre, but less than 60 per cent total digestible nutrients. Contrary to this, concentrates have less than 18 per cent fibre, but more than 60 per cent total digestible nutrients. Roughages may be further sub-divided intosucculent and drytypes.Whereas, concentratesare sub-dividedintoEnergy or basal feeds havingless than 20 percent crude protein and Protein supplements havingmorethan 20percent crude protein. Followingfeeds indicate theoutlines of classification [Flow chart 1] of the conventional feeds into broad categories with examples.

The bio-degradation process dates back to time immemorial when some wild ruminant animals were spotted feeding on the tree –woods in South Chile. The natives of this place called this feed as “Palo Podrido (Huempe)”, meaning – ‘animal feed’, as opined by F. Zadrazilof Germany. Later on, it was found that thiswood was actuallydegradedbycertaintypes offungi undernatural conditions and decomposed to Spent-wood, which turned out to be the animal feed. The scientists started working on these fungi as well as this type of process. Lately, the fungi were found to be belonging to higher basidiomycetes class, called: White-rot-fungi. They mainly degraded lignin of the wood, however cellulose and hemicelluloses to a limited extent only.

But, surprisingly it was found that the digestibility and crude protein content increased to a considerable extent. Later on, the scientists started working on spent-straws too. The cereal straws (lignocellulosics) constituted a staple-feed (basal) in the developingcountries for theseruminantanimals. But, thepresence of lignin (a biological plastic) limits the utilization of these cereal straws in the rumen eco-system. Thus, the concept of SolidState Fermentation (SSF) sparked into this type of investigation. Some examples of such white-rot-fungi were: Pleurotus ostreatus, Coprinus cineus, Sporotrichum thermophile, Phanerochaete chrysosporium, Polyporous berkeleyi, Neurospora crssa, Alternaria tenius to name a few. Some examples of substrates upon which such types of fungi grew for SSF were: wheat straw, paddy straw, paddy husks, ground nut shells, sugarcane bagasse, barleystraw, sunflower stalk, aspen wood, birch wood, saw dust etc. The resurgence of interest in the use of straw from cereal and grain crops as animal feed had been witnessed in early nineties to maintain the performance of animals. Following were some of the variations undertaken by different scientific groups:

1. Introduction

The term nanotechnology was first coined by Norio Taniguchi in 1974 to describe controlled thin film deposition (Taniguchi, 1974). Nanotechnology is now generally recognized to include materials with a size in at least one dimension in the 1-100 nm range, although this terminology is not strictly enforced (Rao, 2008). In food science, there are many types of naturally occurring particles that fall within this size range and the use of a strict definition is problematic due to the large distribution of sizes (Kruif, 1998). Many natural foods contain nanoscale components and their properties are determined by their structure. These have been eaten safely for generations. In fact, some of food’s most important raw materials (proteins, starches, and fats) undergo structural changes at the nanometre and micrometre scales during normal food processing(Morris and Parker 2008). Food proteins (for example, native beta-lactoglobulin, which is about 3. 6 nm in length) can undergo denaturation (via pressure, heat, pH, etc. ) 

1. Introduction

Jackfruit is the largest edible tree borne fruit in the world (Baliga et al., 2011). According to Haq (2006) India is considered as the birth place of jackfruit. The major jackfruit producing states in India are Assam, Tripura, Bihar, Uttar Pradesh, the foothills of the Himalayas and South Indian States of Kerala, Tamil Nadu and Karnataka. Jackfruit is a fruit prized in some areas of the world and allowed to go waste in others. According to Sidhu (2012) the extent to which jackfruit is being wasted in India is not exactly documented however, the wastage is approximately 75%. On an average a fully developed fruit may weigh 10-25 kg. Seeds occupy around 10 to 15% of the total weight of the jackfruit (Bobbio et al., 1978). It is reported that jackfruit seeds contain high amount of protein, fiber and carbohydrate (Ocloo et al., 2010). The seeds are viable for not more than three or four days (Sidhu, 2012). According to Abedin et al. (2012) the industrial utilization of jackfruit pulp are varied but the seeds are discarded from those food industries. Moreover, mature fresh seeds cannot remain fresh for long as they tend to germinate easily. So, converting jackfruit seed into flour is an option in order to increase the shelf- life of the seed and can substitute various cereal based flours for developing food products. Dumpling is a type of food which is made out of small pieces of dough wrapped around a filling that may include vegetables, meat, or other ingredients mixed together.

1. Introduction

With increase in consumer awareness and lifestyle the interest for health promoting natural food ingredients has expanded in recent years. However, globally it has become a great challenge to the manufacturers to ensure that such compounds should remain in their active form during processing and storage with an emphasis to its bioavailability profile. Oxidative stress is the major contributor to the pathogenesis of a number of chronic and degenerative diseases including atherosclerosis, Alzheimer’s and Parkinson’s disease, diabetes, DNA damage, carcinogenesis, cardiovascular and neuro degenerative diseases, allergies, premature ageing etc. (Gupta et al. , 2014; Svetli et al. , 2010). Endogenous antioxidant defense mechanisms are not always sufficient to fully scavenge the free radicals formed in the body during various processes. Therefore, dietary components having antioxidant properties, such as tea flavonoids, are very much necessary to supplement the activity of endogenous antioxidants. Tea is one of the most concentrated sources of flavonoids and phenolic acids which show antioxidant activity by sequestering metal ions and by scavenging reactive oxygen and nitrogen species (Frei & Higdon, 2003).

1. Introduction

Kiwi fruit (KF) is very much acceptable for preservation by freeze-drying (FD) technique, since it has very much health benefits such as low calorific value, low glycemic index (GI), more vitamins (particularly vitamin C), maximum load of potassium, presence of variety of pigments, few important enzymes, low allergic response (AR) and finally it is rich in natural antioxidants, polyphenols. It is translucent, green pulp and many black seeds arranged nicely with covered by hairy and brown skin. The KF seeds are small sizes and black colored surround inner circularly through the whole pulp. It gives a nice crunch during consumption and provides a number of essential nutrients. Therefore, it will be remembered that seeds cannot be discarded during processing of KF unlike other fruit (jamun etc. ) seeds.

1. Introduction

Underutilized fruits and vegetables are commercially neglected but recognized as rich sources of nutrients and non-nutrient bioactive compounds (Ali et al., 2008). These crops are very popular among local communities in many parts of the world and well accepted in their traditional diets and therapeutic medicines (Animesh et al., 2010). Nowadays, these are gaining significance in research and development programs of many organizations. Meghalaya is endowed with rich and unique diversity of indigenous wild edible fruit species of which most of them are less known and underutilized (Haque et al., 2009). There are rich in functional bioactive components such as vitamins and minerals are the much talked dietary nutrient for women and children health. It is being supplied to body through tablets, vegetables, fruits and other food items (Islam et al., 2012).

1. Introduction

The emergence of food borne illness due to contaminated food is one of the most frequently occurring event and challenge for the food processing industry. This has resulted into economy as well as energy requirement to counter the food borne pathogens. Some of the important pathogenic microorganisms of concern are E. coli, Listeria monocytogenes, salmonella, etc. To address the food safety and quality issues, several non-thermal food processing technologies have been developed in the last decades, with an aim of achieving food safety without significantly affecting the quality of the food product. Cold plasma in one of the non-thermal processing technology, which has shown promising results as evidenced by recent scientific investigations.

The plasma is known as fourth state of matter after solid, liquid and gas state. Plasma contains diversity of energetic species including ions, electrons, atoms, excited molecules and charged particles. In a plasma, a significant number of electrons have such a high energy that no nucleus can hold them. When a gas is subjected to heat or an electric field, some of the atoms become ions and the gas is said to be ionized. An ionized gas in its normal condition, can conduct electric current to a limited extent. If the heat or electric current becomes extreme, many of the atoms become ions. The resulting super ionized gas, which can conduct a large and sustained electric charge. Plasmas are used in several materials processing industries like textiles, electronics, paper and glass industries etc.

1. Introduction

Sundarbans is claimed to be one of the wonders of the world for its natural resources of halophytic mangrove forest (some 34 species of true mangroves are present), biodiversity and ecosystem that form an extraordinary environment of the globe. Available brackish water environment of Sundarbans provides favourable substratum for algal communities (Naskar and Naskar, 2010). Enteromorpha intestinalis specifically is one of the algal species that is very abundant in the Sundarban region. The production of total seaweeds in India in 2000 was approximately 600,000 tons (wet weight). India produces 110-132 tons of dry agar annually utilizing about 880-1100 tons of dry agarophytes. The seaweed industry in India is mainly a cottage industry and is based only on the natural stock of agar-yielding red seaweeds, such as Gelidiella acerosa and Gracilaria edulis, and algin yielding brown seaweeds species such as Sargassum and Tubineria (Kaladharan and Kaliaperumal, 1999).

Investigational studies indicate that marine algae contain significant amount of nutraceuticals, vitamins, proteins, carbohydrates, fiber and antioxidants. Composition indicates that there is a tremendous scope for food and pharmaceutical applications. These may be used for food fortification but after rigorous studies. Formulation, standarizaiton and characterization should be performed before human use. Studies for exploitation of the bioactive components of the species may open up new avenues. Hence there seems to be a full scope for the realization of outcomes of the research. Further the utilization of the developed processes at higher scales may reap in financial benefits and may prove beneficial to the local people, who are supplier of the raw materials. The present study is an attempt to utilize this India variety of macro-algae for fortification of bakery food products.

1. Introduction

Okara is a by-product generated during processing soybean into soymilk or tofu. Okara is rich in fibre and protein. It contains most of the carbohydrates, some of the protein and a small portion of the oil from soybeans. Okara contains approximately 50% fibre, 27% protein, and 10% fat on dry basis (Li et al., 2012). About 1. 2 kg of fresh okara is produced from every kilogram of soybean processed for soymilk (Vishwanathan et al., 2011). Fresh okara from soymilk production has about 80% moisture. The protein present in okara has good nutritional quality and superior protein efficiency ratio. Other soy components that are also likely present in okara include isoflavones (genistein and daidzein), lignans, phytosterols, coumestans, saponins, and phytates (Li et al., 2012). The most common method of utilization of okara is in the form of animal feed. Tofu or soymilk processing plants primarily dump okara as landfill or resort to burning. Okara can be utilized as a fertilizer as it contains various nutrients required for growth of plants. It can be used as a fermentation substrate for production of ethanol. Okara can also be used in pet foods as it contains high levels of extenders and protein. Apart from these, okara can be used in a variety of food products. It can be used in various food products. Utilization of okara is still in the initial stage. It can be used as a replacement of wheat flour in baked products. It can also be used in various soups, salads or vegetable preparations.

Resistant starch refers to the portion of starch and starch products that resist digestion as they pass through the gastrointestinal tract. Resistant starch is the fraction of starch which is not hydrolysed to D-glucose in the small intestine within 120 min of being consumed, but which is fermented in the colon. It can be used as a functional ingredient in various food formulations (Fuentes-Zaragoza et al., 2010). RS has desirable physicochemical properties (Fausto et al., 1997) such as swelling, viscosity increase, gel formation and water-binding capacity, making it useful in a variety of foods. It helps in improving the appearance, texture, and mouthfeel of the final product compared to conventional fibres. Resistant starch can act as a prebiotic for gut microflora (Sajilata et al., 2006). It has hypocholesterolemic effects, is hypoglycaemic and prevents gall stone formation.

1. Introduction

Organophosphate (OP) and organocarbamate (OC) pesticides are highly used in modern agriculture due to their low persistence and high insecticidal activity. Although they are relatively less persistent than the organochlorines, they have greater acute toxicity with tolerance level ranging from 1 to 8 μg mL-1 (Mulchandani et al., 2001). The high toxicity of OP and OC neurotoxins and their large use in modern agriculture practices has increased public concern, health risk and the consequent contamination of water and food sources (Buonasera et al., 2010). Development of fast and cost effective analytical tools for onsite monitoring of OP and OC contaminations in food, water and in the environment is highly necessary. An approach toward that has resulted the evolution of acetylcholinesterase (AChE) based biosensors for OP and OC pesticides. Ample literature is available on AChE based biosensors (Zhou et al., 2013). But some intrinsic disadvantages remain that limits its utility and field deployment. The most influencing ones are their inability to sustain in organic solvents and the limited class selectivity that demands alternate bioreceptor for pesticide detection. Given the diversity in mode of action of different classes of pesticide, it is quite challenging to come up with different sensor molecules for each class of pesticides. As a continuous effort towards that goal, attempts have been made to use the Glutathione S –transferses (GSTs; EC 2.5.1. 18) for the purpose. GSTs are a multigenic family of cytosolic proteins with multifunctional biological roles, widely distributed throughout the body and found in the liver, kidney, brain, pancreas, testis, heart, lung, small intestine, skeletal muscles, prostate and spleen (Jancova et al., 2010). The important biological functions those are making GSTs the focus of current research includes its catalytic role in detoxification reaction (Lumjuan et al., 2011) over expressions during phase II metabolism as well as drug resistance associated with anticancer therapies of human and the resistance acquired by certain insects while getting exposure to pesticides (Borah et al., 2016).

1. Introduction

The culinary banana (Musa ABB) of Assam and North East India (locally known as kachkal) is an important and cheap source of vegetable in the diet of local people. It is considered as one of the potential sources of carbohydrates, starch, polyphenols, micronutrients and functionally important bioactive compounds (Khawas et al., 2016). Native starches have limited use in food application as it has many weakness such as narrow peak viscosity range, poor process tolerance, low shear stress resistance, hydrophilicity, thermal decomposition, high retro gradation, and synersis and cooked starches will formed a weak, cohesive and rubbery paste. These facts motivated the employment of modified starches as important functional ingredients in processed foods in recent years because of their improved functional properties over unmodified starches (Fleche et al., 1985).

To overcome these problems physical. Chemical and enzymatic modification of Starch is done. Chemical modification of starch generally involves esterification, cross linking, etherification or oxidation of the available hydroxyl groups on the β-D-glucopyranosyl units that make up the starch polymer.

Introduction

Proteins are the most abundant organic compounds constituting major part of the body. Chemically, proteins are nitrogen containing macromolecules and polymers of L α-amino acids, which serve a wide range of structural and biological functions in animal body. For example, connective tissue made up of collagen, metabolicenzymes, neuro transmitters,signal in gmolecules, structural components of cell membranes and cell organelles, etc. all are chemically proteins.

Before goinginto the details of protein metabolism, first, we need to understand that there are majorly two sources of proteins in our body: dietary protein and body protein. Dietary protein is taken in diet, while body protein indicates the protein and amino acids synthesized in animal body to fulfill the requirements for growth and development. The digestion of proteins will not be discussed here as it has been described in detail in chapter 12. Dietary protein is digested in presence of several enzymes such as trypsin, pepsin, chymotrypsin, aminopeptidases and carboxypeptidases and is converted into mono-, di- and oligopeptides in stomach and small intestine; and finally converted into amino acids. These amino acids are transported to the systemthrough intestinal mucosa via active transport, from where they enter into the cells for further metabolism. Therefore, ‘metabolismof proteins’ is more appropriatelylearnt as ‘metabolism of amino acids’.

As homogeneity is a basic criterion, it is important to know the solubility of solutes, which is a temperature dependent property. Besides temperature, solubility is influenced by the acidity (pH) of the solvent due to the acid-base character of the solute. Bases tend to dissolve better in acidic solutions, whereas acids do in basic solutions. Therefore, one often needs to change the pH of solutions accordingly. As the most widely used concentration units (molarity, w/v% and v/v%) refer the amount of solute in a given volume of the solution, it is the volume of the complete solution that must be set to the desired (calculated) value thus, one must consider not only the solvent but the solute(s) as well. Another reason why one must add the solute(s) to an amount of solvent which is less than the final volume might be that one will need to set the pH subsequently in order to enhance solubility and/or to reach a desired pH value. The volume of acid or base solutions used for pH setting is usually not known in advance and is not negligible. If the solute was initially dissolved in the final desired volume, one would exceed this volume during pH setting (even if the volume of the solute is negligible) and, thus, the solution would not have the desired (calculated) concentration but a smaller (and mostly unknown) one. Thus, according to the correct procedure, one must take into consideration the fact that the final volume will contain the volume of the buffering component. value particularly in the case of dissolving proteins, which have a tendency to stick to the bottom of the dish, thereby making dissolution substantially slower.

There are other groups that contribute to the reactivity of enzymes beside amino acid residues.

These groups are called cofactors - chemicals required by apoenzymes (inactive) to become holoenzymes (active).

There are two types of cofactors:

1. Essential ions - metal ions -inorganic
2. Coenzymes - organic molecules that act as group-transfer reagents (accept or donate groups)- can also be H⁺ and/or e^-

Both provide reactive groups not found on amino acids side chains.

Coenzymes can be either cosubstrates (loosely bound to enzyme; is altered, then regenerated) or prosthetic groups (tightly bound to enzyme).

Coenzymes can be classified by their source:

NEW CELLS ARE PRODUCED BY DIVISION OF PREVIOUSLY EXISTING CELLS

All cells come from previously existing cells

To make a new cell identical to itself a cell must perform 4 tasks:

It must grow and make additional copies of all its organelles, enzymes, etc..

It must duplicate its DNA

It must accurately separate the DNA into 2 units, so that each cell gets a full set

It must divide into 2 cells (cytokinesis)

SEX IS VERY COSTLY

Large amounts of energy required to find a mate and do the mating: specialized structures and behavior required

Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.)

Genetic costs: in sex you pass on only half your genes to your children

Males are an expensive luxury- in most species they contribute little to rearing offspring

But There are Some Advantages

More genetic diversity: more potential for survival of species when environmental conditions change

Shuffling of genes in meiosis (random separation of homologous chromosomes)

Crossing-over in meiosis (see below)

Fertilization (genes from 2 individuals brought together)

A fair number of practical breeding experiments were done in ancient times

People knew there was a connection between sex and reproduction

The idea had developed that “like begets like”

By selecting certain plants and animals and breeding them many domestic varieties of plants and animals had been developed

Example: corn was developed from a wild grass in Mexico and Central America, probably about 5000 years ago

149 of the top 150 plant crops were domesticated by primitive man

BACTERIA HAVE SMALL, COMPACT GENOMES

We now know the complete DNA sequences for about a dozen bacteria

Bacteria seem to need about 500 to 5000 genes to conduct their lives

Because they are small, bacterial genomes are highly organized

Most of the time there are only a few bases between the end of one gene and the beginning of the next

Few introns are found in bacteria

Bacteria contain accessory “chromosomes” called plasmids; each has only a few genes

I. Definition

Solute transport in plants, translocation, primarily occurs in the phloem, but it can occur in the xylem.

II. Solute Transport in the Xylem

Some solutes are transported in the xylem

Water and dissolved ions are the main substances in vessels/tracheids

These materials are transported via transpiration stream

Xylem sap may also contain organic materials, usually in relatively low concentration (with a notable exception being maple sap in the spring which is comprised of 2% or more sucrose).

Substances move at different rates depending on matrix effects, metabolic needs, etc.

PLANT NUTRITION

Plants use inorganic minerals for nutrition, whether grown in the field or in a container. Complex interactions involving weathering of rock minerals, decaying organic matter, animals, and microbes take place to form inorganic minerals in soil. Roots absorb mineral nutrients as ions in soil water. Many factors influence nutrient uptake for plants. Ions can be readily available to roots or could be “tied up” by other elements or the soil itself. Soil too high in pH (alkaline) or too low (acid) makes minerals unavailable to plants.

INTRODUCTION

The weather conditions during the growing season of crops influences the onset and development of disease. The relationship between weather and plant disease development is the basis for meteopathological forecasting. On the basis of observed or forecast weather conditions, the incidence of plant disease is likely to be predicted.

Such predictions are designed to warn growers of expected significant developments in the manifestation of a particular plant disease. Such disease forecasts, if sufficiently accurate, permit efficient and competent counter measures to be adopted. A short range or medium range of disease forecast, issued at short intervals during the growing of crops, can give guidance to the farmers on the best remedial measures. Growers can decide whether a chemical control measure is needed or not, and if needed then optimum date on which to be applied and similar day to day decisions to fight against disease to minimize the losses to be caused.

The result, of adopting such a plan of weather based control measures, is to reduce the number of chemical sprays to a well timed few, thus giving an equal or even better protection at a lower cost in chemical and labour, and simultaneously reduces losses caused by phytotoxic and mechanical damage to the crop. The reduction in the use of potentially toxic chemical is a matter to be welcomed in the present world, considering the consequences of the widespread use of toxic chemicals.

INTRODUCTION

Mizoram is a hilly terrain lying in the north eastern part of the country. The total geographical area of the state is 21, 087 sq. km and lies between 92º 15/ to 93º 29/ E longitude and 21º 58/ to 24º 35/ N latitude. Out of six agro climatic zones of the NEH region, Mizoram comes under sub tropical hill zone and posses a sub tropical and humid climate due to high rainfall (Fig. 1). These climatic conditions are very conducive for the cultivation of a large number of agri-horticultural crops. The physiography of the state is divided into hills and valley lands. About 90 % area of the state comes under hills and only 10 % area is under valley. The valley lands are sporadically distributed in small patches except in Champhai district. The slope of the hills in Mizoram is much steep as compared to the other states in the North Eastern region. The land use statistics of the state is presented in Table1.

INTRODUCTION

Edible mushrooms are higher fungi occurring seasonally all over the world in various habitats varying from sandy plains to thick forests. About 2000 fungal species in the world have been identified as edible, of which, only a few (20) are cultivated commercially (Chang and Miles, 1991). These mushrooms are generally considered as the good source of protein, vitamins and minerals in addition to their flavour and condiment value. About 3.

My definition of a ‘Developed India’ is “when the quality of life in our rural areas becomes comparable to that in the non-urban areas of already developed countries”. Another way of looking at development is to look at ‘Measures of Development’. Unlike the three used by United Nations, viz. per-capita Gross National Product, life expectancy at birth and adult literacy, I have been suggesting for a long time that the appropriate measures of development for a developing country are only two – per capita electricity consumption and female literacy. Availability of electricity is essential to provide the basic amenities like pure drinking water and proper health care and to develop rural industries. And also, without near-hundred per cent literacy and without societal equity – evidenced inter-alia through gender non-discrimination – the fruits of science and technology (S&T) will not fully reach rural India.

Technology Foresight to Become a ‘Developed’ Country

Choosing the right technologies to put the nation on the part of rapid development requires technology Foresight. Technology foresight must be distinguished from Technology Forecasting, which attempts to predict future technological developments, without worrying about the impact of these developments on the economy, society or the environment. Technology foresight adds assessments from economic, social, environmental, security and even ethical points of view to technology foresight. One may often get different answers from such analysis depending on whether you are from India or say, from the United States of America, to the need for technologies like, for example, fast breeder reactors or rural food processing.

ABSTRACT

Over the last three decades, SAC has been engaged in the development and dissemination of remote sensing and GIS technologies for national development. These developments have originated from various initiatives related to national projects, sensor related research and in-house research and development on applications. A large multidisciplinary team of scientists develop technologies in various fields of applications like agriculture, coastal zone management, urban planning, forest and environment, etc. In addition, significant efforts are directed towards technology development for generic applications like photogrammetry and applications involving synergistic usage of remote sensing, GIS, GPS and satellite communication. Some specific examples of recent technological developments pertain to simulation modeling of crop yields, horticultural crop management, site selections for aquaculture, location of cold storage plants, large area crop production forecasting, base map creation, telegeoprocessing, mobile satellite services for fleet management, automated procedures for linear infrastructure planning, urban land use allocation planning.

An overview of these technologies and the modalities for their effective utilization by government, industries and users are summarized. Dissemination of these technologies for public bodies and private sector has been taken in the form of promotion of consultancy services in different application areas. Some major areas where these sectors have benefited through consultancies are estimation of cash and other field crops, city development planning, site selection for industry, land use change detection studies for environmental impact assessment, coastal regulation zone compliances and land use scenario, forestry related applications like catchment area treatment, forest fires monitoring, etc. Examples of consultancy services for private sector are included.

Keywords : Technology dissemination, technology development, remote sensing applications.

Agarose gel electrophoresis is the easiest and commonest way of separating, capable of resolving mixtures of DNA fragment that cannot be separated conveniently by other procedures. The purpose of the gel might be to look at the DNA, to quantify it or to isolate a particular band. The DNA is visualised in the gel by addition of ethidium bromide. This binds strongly to DNA by intercalating between the bases and is fluorescent meaning that it absorbs invisible UV light and transmits the energy as visible orange light.

Agarose, which is extracted from seaweed, is a linear polymer whose basic structure is shown in figure.

Principle

Monomeric acrylamide (which is neurotoxic) is polymerised in the presence of free radicals to form polyacrylamide. The free radicles are provided by ammonium persulphate and stabilised by TEMED (N’N’N’N’-tetramethylethylene-diamine). The chains of polyacrylamide are cross-linked by the addition of methylenebisacrylamide to form a gel whose porosity is determined by the length of chains and the degree of crosslinking. The chain length is proportional to the acrylamide concentration: usually between 3.5 and 20%. Cross-linking bisacrylamide is usually added at a ratio of 2g BIS 38g acrylamide.

Principle

The SDS polyacrylamide gel electrophoresis (PAGE) performs:

Separation of proteins according to their molecular weight

Ascertain whether a given protein is actually present in a sample,

Assessment of purity of the preparation

Estimation of the approximate quantity of the protein, and

Measurement of the size of the protein.

Principle

Western blot analysis (also called protein immunoblot) is a useful tool for the detection of proteins in biological samples. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose), where they are probed (detected) using antibodies specific to the target protein.  It is a technique for analyzing and identifying protein antigens: the proteins are separated by electrophoresis in polyacrylamide gel, then transferred (“blotted”) onto a nitrocellulose membrane or treated paper, where they bind in the same pattern as they formed in the gel. The antigen is overlaid first with antibody, then with anti-immunoglobulin or protein A labeled with radioisotope, fluorescent dye, or enzyme. 

The method originated from the laboratory of George Stark at Stanford. The name “Western blot” was given to the technique by W. Neal Burnette and is a play on the name “Southern blot”, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blotting and the detection of posttranslational modification of protein is termed “Eastern blotting”.

Origin and Distribution of Camel

A camel is an even-toed ungulate within the genus Camelus of the family Camelidae bearing distinctive fatty deposits known as “humps” on its back. There are two species of camel, viz. Camelus dromedarius (single humped dromedary) having habitat in dry hot arid lands of Africa and Asia, and Camelus bacterianus (double humped or bactrian) habitat of cold arid lands of Central palaerctic (a zoogeographical region consisting of Europe, Africa north of the Sahara, and most of Asia north of the Himalayas) region. Family Camelidae also includes other species which are camel like mammals. Those are Lama (Lama glama), Alpaca (Lama pacos), Vicuna (Vicugna vicugna) and Guanaco (Lama guanicoe). These four species are termed as new-world Camelidae having habitat in South America. The Indian camel belongs to Camelus dromedarius. A very small numbers of double-humped camels are also available in Ladakh area of Jammu and Kashmir.

Camel is an important component of desert eco-system. It is capable of traveling long distance in hot, arid and sandy regions carrying men, material and providing energy to agricultural operations. They provide milk, meat, hair and used as work animals. It is believed that Camels were domesticated during the period from 3000 to 2500 BC. Average life expectancy of a camel is 40-50 years. The height at the shoulder and hump of a fully grown adult camel is 1.85 and 2.15 metres, respectively. Camel can run at a speed of up to 65km/h for a short distance and can sustain a speed of up to 40km/h for long distance.

INTRODUCTION

Crossandra is an important traditional flower crop of south India. It is small evergreen shrub that is quite hardy and has the ability to produce beautiful flowers with a remarkable range of colours almost all the year. This has made it extremely popular in southern states of India where the flowers are widely used in temple offering and for making gajras and venis to use as hair adornments.

Owing to their attractive colours, light weight and good keeping quality, crossandra flowers are ideal for garland making. Even though the flowers lack fragrance, they are widely used in garlands either alone or along with jasmine to produce charming colour contrasts. Steady market demand and assured and regular income have made crossandra a profitable venture for south Indian farmers.

INTRODUCTION

Freesias are extremely popular bulbous plants that are best known as florist flowers. They bear bell-shaped tubular flower and are grown commercially on a vast scale. They are grown both for flower trade as well as in house gardens. Freesias are suitable for pots, shallow pans, beds, indoor decoration and window boxes. Freesia flowers are prized for their curious elegant form, peculiar arrangement of the blossoms on their stalks and a vast range of delicate colours ranging from creamy white to yellow, orange, red, purple, brown, lavender and vermillion. They are highly valued cut flowers.

Freesia flowers are so strongly scented that only a few potted or cut blossoms can bring fragrance to an entire room. Its unusual appealing shapes make them suitable line flowers for any arrangement. They also make fragrant additions to bouquets and their wide range of colours increases their versatility for design uses.

INTRODUCTION

Lotus is the foremost symbol of beauty, prosperity and fertility. According to Hinduism, within each human inhabiting the earth is spirit of sacred lotus. It represents eternity, purity and divinity and is widely used as a symbol of life, fertility, ever-renewing youth and to describe feminine beauty especially the eyes. Egyptians considered it a symbol of rebirth, since it closes in the evening and falls to the water, then reopening again in the morning, thus emulating the behavior of the sun. All lotuses are day-bloomers that open early in morning and close by mid afternoon for three successive days, with first flower closing earlier. It possesses an amazing ability to flourish in a variety of environment ranging from clear ponds to muddy marshes. The exquisite flowers with long herbaceous stalk arise from the rhizomatous stems and a flat and peculiar seedcase at its center.

Lotus, the national flower of India, has been a part of Indian culture, religion and history from ancient times. It is considered sacred not only by the Hindus of India but also by the Buddhists of China and Japan. To early Egyptians, a lotus bud or Sesen was a symbol of rebirth. Lotus is offered to Goddess Lakshmi and is considered as her symbol. Besides, lotus has found place in one of the postage stamps in India. Every year some prestigious awards are given to the distinguished personalities of the country like Padmashree, Padmabhushan, Padmavibhushan and Swarna Kamal (Golden Lotus).

Biotechnology can be defined as utilization of materials and methods for benefit of mankind by the utilization of microorganisms and biological agents. It is an area with a tremendous potential in solving basic problems of food, fibre, fuel and medicines in developing countries. According to the office of the Technology Assessment of the US Congress, biotechnology can be defined as any technique that uses living organisms to make or modify a product, to improve plants or animals or to develop micro organisms for specific uses. Flower industry has always increasing demand for new forms, colours and types of flowers. Biotechnology has made significant advances in the improvement of ornamental crops and development of new varieties has great potential to be utilized in future. Utilization of this technology can be done in floriculture under two broad subheads viz., tissue culture and genetic engineering.

TISSUE CULTURE

The major commercial utilization of tissue culture has been micropropagation, which is an alternative to vegetative propagation. Micropropagation permits true to type multiplication of a desired genotype using in vitro culture techniques. Micropropagation process can produce a large number of propagules in a relatively short time and space under controlled conditions. It has already revolutionized production process of several nurseries. The dream of multiplying disease free plants in required quantity on the specified time schedule in an industrial way has become true due to micropropagation. Some of the important floricultural crops that are successfully propagated in vitro in Indian laboratories are given below with their explant source:

Carnation: Leaf segments, internodes, petals

Gerbera: Leaf, petiole, flower stalk segments

Gladiolus: Inflorescence stalk, leaf sections

Orchids: Epidermal peelings, leaf segments, root tips

Rose: Internodal segments, petals, leaf segments, immature embryos, root segments