eBook Chapters

The following scientists greatly contributed to the wind driven circulation. Out of all Ekman, Sverdrup, Stommel and Munk are most important and ever remembered for their work. So in the following pages discussion is mostly concentrated on these four scientists theories.

El Nino is the name given to the occasional development of warm ocean surface waters along the coast of Ecuador and Peru. When this warming occurs the usual upwelling of cold, nutrient rich deep ocean water is significantly reduced. El Nino normally occurs around Christmas and lasts usually for a few weeks to a few months. Sometimes an extremely warm event can develop that lasts for much longer time periods. In the 1990s, strong El Ninos developed in 1991 and lasted until 1995, and from fall 1997 to spring 1998.

El Nino means ‘The Little One’ or ‘Christ Child’ in Spanish. It was originally recognized by fisherman of the coast of South America as the appearance of usually warm water in the Pacific Ocean, occurring near the beginning of the year i.e. around Christmas. That is why the name is used. El Nino, the periodic warming of Pacific Ocean waters that affects the weather worldwide. C. Fred and T. Andrus of the University of Georgia studied the ancient fish bones from refuse left about 6000 years ago by ancient peoples in Peru and found that ocean catfish lived in water that averaged 60 to 70 warmer than now and that there was little variation in temperature. He concluded that if El Nino was occurring at the modern rate, once every two to seven years, then the bones from the fish would have reflected the temperature variation.

1. Botanical name : Elaeocarpus serratus

2. Family : Elaeocarpaceae

3. Common Name : Ceylon olive and Wild olive

4. Name in Indian languages: Veralu (Sinhala) and Karai or Ulankarai (Tamil).

5. Description

Elaeocarpus serratus, also called, Ceylon olive is a tropical fruit . The tree is a medium size tree with leaves similar to avocado. The tree usually measures from 8 to 15 metres in height. The fruits are almost similar to the large green olives. This is a sun loving tree grows on Loam soil and requires low maintenance. The delicate, lacy, white flowers usually attract a myriad of insect pollinators, notably flies, wasps and flower beetles.

8.1 Introduction

Cartilaginous fishes have ventrally placed mouths with teeth in several series. They possess rigid dorsal fins and placoid scales. They have five pairs of gill clefts opening individually to the exterior, a lower jaw articulated with the upper jaw and upper jaw not fused to the skull, no swim bladder, an operculum, an endolymphatic duct and a heterocercal or homocercal tail. It shows internal fertilization, meroblastic cleavage and direct development.

Elasmobranchii includes a wide range of cartilaginous fishes. Dogfish sharks are commonly studied all over the world probably because of their suitable size for dissection.

Marshall was the frst to establish the idea of elasticity. According to the law of demand, if the price increases, fewer units will be sought for. But it’s crucial to know how much less will be expected of it. Such inquiries are crucial when formulating public policy. For instance: A price rise for salt might not have much of an effect on demand, but a price increase for TV will have more of an effect. Why? The idea of elasticity provides an answer to such queries. In economics, the concept of “elasticity” is frequently used to describe how responsive one variable is to changes in another.:

Elasticity of demand

Demand elasticity generally refers to demand’s sensitivity to price. However, there are as many levels of elasticity as there are demand determinants. The most important Elasticities are:

Determinants of demand Elasticity

8.1. Meaning
The Law of Demand states that when the price of a good increases, its quantity demanded decreases, assuming all other factors remain constant. However, the law does not specify how much the quantity demanded will decrease in response to a price increase or how sensitive demand is to price changes. This information is crucial for businesses and policymakers. To address this, Alfred Marshall developed the concept of elasticity of demand, which measures the responsiveness of quantity demanded to changes in factors such as price, income, and the price of related goods.
8.2. Type of Elasticity of Demand
There are three types of elasticity of demand viz., 01) Price Elasticity of Demand 02) Income Elasticity of Demand 03) Cross elasticity of Demand
1. Price elasticity of Demand
Price elasticity measures the responsiveness of the quantity demanded of a commodity to changes in its price, while keeping all other factors constant (ceteris paribus).

Definition : It is a technique in which foreiirnb is amputed from elbow joint. Indication : Incurable damage of radius ulna.

Electric Cooking Range is also known as an electric stove or electric range; is a stove with a built-in cooktop and with an integrated electrical heating device to cook and bake. Electric stoves became popular as replacements for solidfuel (wood or coal) stoves which required more labor to operate and maintain. “Cookstoves” (also called “cooking stoves” or “wood stoves”) are heated by burning wood or charcoal; “gas stoves” are heated by gas; and “electric stoves” by electricity. Unlike gas stoves, electric stove tops don’t produce a flame.

• Structure and working of electric organs in- Electric eel, electric catfish, electric ray.

• Mechanism of electric discharge formation and control of electric organs.

Fishes possess a number of adaptive structures which have been evolved to meet special requirements faced by them. The electric organs are highly specialized structures that enable their possessors to produce store of discharge to electric current. Fishes are unique in the animal kingdom in being capable of generating an electric current.

About 250 species of both, the cartilaginous and bony fishes of fresh and marine waters are known to possess the electric organs. Most of these restrict to the tropical fresh water of Africa and South America. Many species of rays, skates, and the eels are known to possess small electric organs that give a weak discharge. These fishes can produce electric field outside the body. Electric fishes have been divided into two types-strongly electric and weakly electric, depending on the strength of the current.

18.1 Determination of Electrical Conductivity of Soil Solution

The conductivity or more precisely the specific conductance of a soil at a given soil-water ratio is the reciprocal of the specific resistance offered by the solution at 25ºC between the electrodes of 1 sq cm cross section kept 1 cm apart. Thus, the electrical conductivity of any solution depends on the amount of soluble salt present in it. Knowledge on the extent of salinity is important for management of saline soil including the choice of the crop suitable for that soil. Soluble salt content in saturation extract is more reliable index of salinity level than in soil: water ratio of 1: 2 or 1: 2.5, because it simulates the extent of salinity experienced by crop in field moisture condition.

Salinity class of soil is based on the electrical conductivity (EC) of saturation extract at 25ºC. However, EC of water extract from 1:2 soil-water suspension can be transformed into the EC of saturation extract following the formula (except for soil containing gypsum).

11.1. Determination of Electrical Conductivity of Soil Solution

The conductivity or more precisely the specific conductance of a soil at a given soil-water ratio is the reciprocal of the specific resistance offered by the solution at 25ºC between the electrodes of 1 sq cm cross section kept 1 cm apart. Thus, the electrical conductivity of any solution depends on the amount of soluble salt present in it. Knowledge on the extent of salinity is important for management of saline soil including the choice of the crop suitable for that soil. Soluble salt content in saturation extract is more reliable index of salinity level than in soil: water ratio of 1: 2 or 1: 2.5 because it simulates the extent of salinity experienced by crop in field moisture condition.

Salinity class of soil is based on the electrical conductivity (EC) of saturation extract at 25ºC. However, EC of water extract from 1:2 soil-water suspension can be transformed into the EC of saturation extract following the formula (except for soil containing gypsum).

Pure water is a poor conductor of electricity. Water may carry electricity fairly well when it contains electrolytes such as Acids, bases and salts. Electrolytes in the solution dissociates with cations and anions and pass on electrical conductivity. Hence, more electrolytes concentration in water, more its electrical conductance. The following ions are crucial for adding conductivity to water.
i) Cations: Mg 2+,Ca2+, Na+ and K+
ii) Anions: Cl-, S4
-, CO3
-, HCO3
-and NO3
-
In the International System of units (SI) the reciprocal of ohm is the Siemens (S) and conductivity is reported as millisiemens per meter (mS/m). 1 mS/m = 10 µS/cm = 10 µmhos/cm and 1 µS/cm = 1 µmhos/cm Principle
The quantity and kind of ions present in a solution affect conductivity, which is a numerical representation of a water sample’s capacity to conduct electricity. Since the majority of dissolved inorganic compounds in water are ionised, they add to conductance. The definition of conductance G is the reciprocal of resistance R.

Pure water is a poor conductor of electricity. Water may carry electricity fairly well when it contains electrolytes such as Acids, bases and salts. Electrolytes in the solution dissociates with cations and anions and pass on electrical conductivity. Hence, more electrolytes concentration in water, more its electrical conductance. The following ions are crucial for adding conductivity to water.
i) Cations: Mg 2+,Ca2+, Na+ and K+
ii) Anions: Cl-, S4
-, CO3
-, HCO3
-and NO3
-
In the International System of units (SI) the reciprocal of ohm is the Siemens (S) and conductivity is reported as millisiemens per meter (mS/m). 1 mS/m = 10 µS/cm = 10 µmhos/cm and 1 µS/cm = 1 µmhos/cm Principle
The quantity and kind of ions present in a solution affect conductivity, which is a numerical representation of a water sample’s capacity to conduct electricity. Since the majority of dissolved inorganic compounds in water are ionised, they add to conductance. The definition of conductance G is the reciprocal of resistance R.

Introduction

Proper electrical wiring and earthing are crucial for laboratory safety and functionality. A laboratory houses several installations/equipment that demand stable/continuous power supply. Inappropriate or faulty wiring and earthing may lead to significant risks, including electrical shocks, damage to equipment, and even incidence of fire. Therefore, understanding the requirements of electrical wiring and earthing is essential for designing, construction, and maintenance of laboratory. This chapter aims to provide a basic knowledge of electrical wiring and earthing necessary for a laboratory. Importance of earthing in mitigating electrical hazards and ensuring integrity of electrical installations are also described.

Basics of Electrical Wiring

Basic understanding of electrical wiring is necessary for laboratory safety and efficient electrical functioning. The potential difference between two electrical points (negative and positive) is known as Voltage, which drives the flow of electric current. This is measured as volt (V). The rate of flow of electricity in a circuit is known as Current (I), which is measured as ampere (A). Current can be either direct (DC) or alternating (AC). Opposition/resistance experienced in the flow of current in circuit is known as Resistance (R) and it is measured in ohms (Ω). Higher the resistance means lesser the current flows at a given voltage. The rate at which electrical energy is transferred/utilized in an electric circuit is known as Power (P), which is measured in watt (W). The Power can be calculated as the product of voltage and current (P = V × I).

13.1. Types

Lamps: Head lamp, plough lamp, indicators

Switches: Safety switch, starter switch, etc

13.2. Components

Battery: A battery provides power to electrical or electronic circuits. The voltage rating of a battery tells how much electrical “pressure” the battery can provide. Battery energy is used to operate starter motor and to provide current to the ignition system during cranking. The electrolyte should always be maintained just below the maximum level, but should not go down below the 1/4^th level.

Alternator: Alternators are electromechanical devices used to convert mechanical energy to alternating current electrical energy. While some of the alternators use rotating magnetic field, some use linear. The functioning of alternators is similar to that of direct current generators. As the magnetic field around a conductor changes, a current is induced in the conductor. The rotating magnet or the rotor turns and cuts the field across the conductors. Thus, an electrical current is produced which causes the rotor to turn. This rotating magnetic field causes an AC voltage in the stator windings. In tractors, alternators are used to charge the battery and to power the electric system when its engine is running. An alternator is superior to a dynamo as it provides more precise output control, requires lesser maintenance, is more compact and requires no cut-out.

Introduction

Clean and renewable energy is a basic requirement for developmental activities. Global energy crisis is caused by increasing world population, escalating demand due to urbanisation, and continued dependence on fossil-based fuels. Non-renewable energy sources like coal, natural gas oil contribute 79 per cent of world electricity generation; only 21 per cent comes from renewable energy sources. Promoting the use of existing renewable energy sources and identifying new environment-friendly technology is gaining importance. Switching to renewable energy sources like wind energy, ocean energy and solar energy is gaining a lot of importance. These green technologies emit less greenhouse gases into the atmosphere and hence are pollution free and immensely available. Recently, investigators have identified plants as weak energy sources. Plants are versatile organisms capable of communicating with each other with a number of signaling mechanisms. It was observed that plants generate electronic signals inside their system. The concept of utilising electronic signals for studying various physiological plant processes was first put forth by Sir Acharya J. C. Bose, an eminent biophysicist, botanist, archaeologist and polymath from India (Fromm and Lautner, 2007). His ideologies and concepts of movements of plants laid the foundation for modern electrophysiology-a branch of physiology that deals with electrical phenomena associated with metabolic functions. Attempts have been made to harvest electricity from free electrons available in plant leaves produced during photosynthesis and from rhizosphere of plants formed as a result of bacterial activity. Currently, a lot of attention is being given to improvise and develop this technology. To proceed forward in this direction, it is very critical to understand the mechanism of electrical signaling in plants.

This chapter on electrocardiograms for self-exercise is designed to provide opportunities to the readers to assess their understanding of reading and interpreting electrocardiograms and to increase their confidence. Each electrocardiogram has its detail and a question to answer. On the reverse side of each electrocardiographic tracing, detailed reading and interpretation is provided to match your assessment and to improve your interpretation skill. It is to be remembered that each electrocardiogram should be evaluated in conjunction with complete data base (history, clinical manifestations, laboratory investigations, radiological observations, and echocardiographic observations).

Electrocardiograph, electrocardiography and electrocardiogram are the three terms commonly associated with ECG or EKG. Though, these terms are interdependent, there is clear differentiation and demarcation between these terms and should not be used as synonym.

Electrocardiograph

The term “Electrocardiograph” refers to an instrument or machine that records surface potentials associated with the electrical activity of the heart to generate a graphic representation, the electrocardiogram (Fig.1).

Electrocardiogram is a visual record of electrical activities going on in the heart during depolarization and repolarization (cardiac cycle). It is one of the simplest and fasted non-invasive diagnostic test available to evaluate cardiac function. Though the heart of large mammals have many similarities, difference in shape, size, positioning in thoracic cavity and innervation of Purkinje f ibers exists in different species of the animals making the electrocardiogram peculiar for that species of animal. Peculiar characteristics that distinguish the electrocardiograms of different species of animals are given in this chapter.

An electrocardiographic unit has a compact electrocardiograph machine, power cord, patient cable, thermal recording ECG paper roll, and conducting gel.

Electrocardiographic Unit

The Electrocardiograph is a sensitive galvanometric device that is capable of recording difference in electric potential up to 1 mV (1/1000 of a volt). The machine has a voltage meter and amplifier combined with a strip recording device (Fig.5) to provide a permanent record. Various basic features viz. running (paper) speed of the machine , sensitivity, lead selector, off/on switch, f ilter etc are displayed on the screen (Fig. 5). Electrocardiographic machine can record electrocardiogram at the paper speed of 25 or 50 mm per second as per our selection. The sensitivity switch controls amplitude of the waves. The amplitude or sensitivity can be selected as 0.5,1. or 2.

Electrocardiography-A noninvasive Technique

Electrocardiography is a non-invasive diagnostic technique routinely used to examine the electrical activity of the heart in health and diseases. It is an important tool for the clinical evaluation of the heart. The technique provides important information regarding heart rate, heart rhythm, impulse formation and its conduction. Heart size can also be assessed to some extent in humans and dogs. The process of electrocardiography leads to generation of an electrocardiogram as a consequence of depolarization and repolarization of heart muscle cells during each heartbeat.

Key Points

• Animals can experience varying levels of electric shock. Lightning typically kills animals instantaneously and causes them to fall to the ground without struggle.Most electric shock deaths are caused by cardiac or respiratory arrest.

• Animals often recover from minutes to several hours, rarely going unconscious. Residual nervous system effects, including sadness, paraplegia, and increased cutaneous sensitivity to painless stimuli, can last for days, weeks, or even months.

Introduction

Lightning strikes, broken transmission lines, malfunctioning electrical circuits, or an animal chewing on an electrical cable can all cause an animal to be injured or killed by high-voltage electrical currents. Lightning strikes are seasonal and occur in thunderstorm-prone regions. Caution should always be maintained when investigating probable electrocution situations because the source of the electrification, such as broken transmission wires, may still be present.

Principle

To retrieve and purify any specific DNA fragment from an agarose gel slice; the expected yield is from 50-75% of the amount in the gel slice.

Time required

Restriction digest - minimum of 2 hours

Electrophoresis - approx. 4 hours

Elution - 2-5 hours depending on fragment size

DNA Purification -2 hours

Abstract

Increasing global demand for ‘fresh-like’ processed food products has forced food industries to investigate into various minimal processing techniques. Food processing in general has been associated with thermal processing, alone or in various combination with chemical and mechanical preservation and processing methods. However, thermal processing methods are energy intensive in nature and tend to reduce product quality and freshness at the cost of shelf-life gain. Advance technique such as microwave, radio-frequency and freeze drying do maintain the product quality but their implementation at industrial scale involves high operational and maintenance cost. An ideal processing method therefore would be one that can process food products without losing its quality, is energy efficient and is easily scalable. In this chapter, we discuss about ‘Electrohydrodynamic (EHD) Drying’, which is can be classified as a minimal processing technique. The heat and mass transfer principles involved in EHD drying have been extensively discussed and so has been the key consideration for designing an EHD drying system.

ELECTROLYTE BALANCE

Electrolytes like sodium, potassium, and chloride are substances that dissolve into posi tively and negatively charged particles when mixed with a liquid. The interaction between these substances, referred to as the ‘dietary electrolyte balance (DEB), can be influenced by either the electrolyte itself or the additional salt source used.

Dietary inclusion of potassium and chloride has shown positive effects on heat-stressed broilers. Among various salt options for broiler diets, sodium bicarbonate and potassium chloride have been identified as the optimal choices, especially during hot summer conditions.

Electrolyte balance is often quantified by the uncomplicated equation Na + K – Cl expressed in terms of mEq/kg of diet. An ideal overall dietary equilibrium ranging from 240 to 260 mEq/kg is generally deemed optimal for normal physiological functioning. The body’s buffering systems play a pivotal role in maintaining a close-to-normal physiological pH, thus preventing deviations in electrolyte levels. The principal function of electrolytes pertains to the regulation of body water and ionic equilibrium. Consequently, the requisites for elements like sodium, potassium, and chloride can’t be viewed in isolation; it’s the collective equilibrium that holds significance. The term “electrolyte balance,” synonymous with acid-base balance, is influenced by three factors:

1. The equilibrium and proportion of these electrolytes in the diet.

2. Endogenous production of acids.

3. The pace of renal clearance.

Diagnosis of plant diseases require some knowledge of light microscopy to examine infected tissues for the presence of plant pathogens. Most of the pathogens and the diseases caused by them can be identified quickly by using different methods such as section or cutting of infected tissue, squashes of scrapping of plant surface. Homecare, the detail study enquires ultrastructural examination of the pathogen.. Electron microscope rely on a beam of electrons produced from a hot filament (e.g. tungsten) and its power of resolution is much greater than light microscope. Electron microscope are of two type

The oxygen content may be measured either chemically or electronically. The normal chemical method is the so-called Winkler method which is a titration method, consisting of adding certain chemicals to the water and observing a colour change which will be directly related to the oxygen concentration.

INTRODUCTION

Knowledge Resource Centres play a very important role in this fast changing e-environment of publishing. Their role includes identification and selection of information, its organization and management, storage, retrieval and dissemination to right users at the right time at right place at right price and in right format. The dawn of information revolution is considered to be one of the marvels of 21st century. It is well known that paper and printing were originated in China in the 2^nd and 5^th century of Christian era, respectively.(1) There is a “ wordless heaven book”, “ published in oral or paper less form among the Chinese. The word less book is actually an adaptation and extension of “the song of baked dough by Pie-wen Lu” (2) of the 14^th century. The “elite” in every generation have access to the timelessly flexibly text of the heaven-book to interpret the contents and to predict, accordingly, the great events of the world, particularly when it is in chaos. The world refers to, of course, the “middle kingdom,” which is China in Chinese. Although the work is only a legend, the wordless heaven-book can be considered as the pioneer prototype of modern electronic publishing and as the earliest idea of the recently developed hypertext.

Another classic concept of e-publishing can be traced in Gullivers Travels by Jonathan Swift first published in 1726. The work has a passage describing a writing machine developed by the Lagadonians in the Grand Academy of Lagado. The Lagadonian writing machine used the method of a fixed syntactic structure and the filling in of that structure with contents by a random procedure (3).

BSTRACT

This paper discusses the various issues and concerns about the electronic information resources, which includes mainly such as e-journals, e-books and e-databases etc. In this digital era, the library should be well equipped with trendy facilities & resources within the sort of books, manuscripts, technical journals, standards, conference proceedings, theses, reports, databases, and all the electronic versions of information resources. Various issues and concerns, which are related with the electronic resources and their management is discussed in detailed in this paper.

Keywords: ICT, Electronic resources, e-journals, Electronic resource management.

1. Introduction

Libraries serve as global repositories of knowledge, like to giant treasure boxes. But librarians are faced with a lot of work as more and more things become digital. Electronic Resource Management Systems (ERMS) can help with that (Prajapati, 2016). These are incredibly useful tools that have completely transformed the way libraries handle digital content. The main goal of ERMS is to simplify librarians' lives. They support the acquisition of fresh digital materials, manage subscriptions and licenses, and even provide online resource locators (Anbu et al., 2013). They essentially act as librarians' superhero sidekicks, ensuring that everything works seamlessly in the digital realm. ERMS play a major role in keeping everything organized. They support librarians in maintaining inventory, determining who can use what, and determining when to update it (Mangrum & Ellen Pozzebon, 2012). Libraries are able to ensure that everyone has access to what they need while adhering to all access regulations in this way. However, ERMS aren't limited to library use. They're also excellent for any library user. By making it simple to locate what you're looking for in the library's digital collection, ERMS addresses that. They keep everything organized so you can quickly get to the essentials (Prajapati, 2016).The key component that keeps contemporary libraries running well is ERMS. They make it easier for librarians to handle electronic resources and for users like you and me to find what we're looking for. Thus, keep in mind that ERMS are the unsung heroes who make it all happen the next time you're navigating your library's digital world.

Introduction

Due to the advent of new technologies now-a-days e-resources became more popular and essential. The information technology has made it possible the availability and accessibility of e-resources. The e-resources are made available both online and offline. Further some are free and some are paid. The free e-resources can be accessed anywhere and anytime on the discretion of the user. But for the paid e-resources to get comprehensive access by using best possible tools and techniques is the library. Multiple resources in the present electronic environment can be seamlessly integrated with a single login gateway which makes is user friendly. Further the explosive growth in network connectivity and rapid advances in computing power systems have altered the speed of communications. Recent advances in the information world have transformed the role of libraries in disseminating information to users. Increase in the number of users and their different needs have made modern libraries apply new communication technology. As a result of this there is a worldwide need and trend to collect, organize, manage, protect and distribute information in digital form.

INTRODUCTION

The goal of the libraries is to provide an effective combination of print, non-print and electronic resources, and the integration of the use of these resources in support of teaching, learning and research at the university. Electronic resources, however, pose challenges not encountered with the acquisitions of traditional library materials, such as access, interface, technical support and licensing. The libraries therefore need to formulate separate Electronic Resources Collection Development policy to address these issues. The purpose of this policy is to provide guidelines in choosing appropriate electronic resources and to establish consistency and priorities in managing this important part of the libraries’ collection.

ELECTRONIC RESOURCES

“Electronic resources” refer to those materials that require computer access, whether through microcomputer, mainframe, or other types of computers, and that may either be locally mounted or accessed remotely via the Internet. These resources cover both free Internet resources and electronic resources purchased or licensed by the libraries from a commercial source, a non-profit organization, a professional organization or any external institution.

INTRODUCTION

Providing access to the nascent information is the main motto of the library. Electronic resources management (ERM) is the latest phenomenon in the field of Library and Information Science. In a nutshell it is responsible for the evaluation, selection, pricing, securing, maintenance and provision of electronic resources to the users. As electronic resource collections continue to expand, allied workflows and processes encompass growth in complexity. Advances in printing technology and information communication technology (ICT) have resulted in transforming the publishing industry into e-publishing. It has resulted in considerable impact on the library collection building process in terms of print collection, electronic and online resources.

Now a days many reputed publishers have started e-publishing and many of them are now issuing their publications in electronic version besides conventional printing. The libraries are upgrading their collection in terms of documentary resources and electronic resources and infrastructure to sustain in the fast moving knowledge society.

Principle

Different ions migrate at different speed dictated by their size and by the amount of charges they carry. As a result, different ions can be separated from each other by electrophoresis. The magnitude of the electric force F exerted on the charged particle when subjected to electrophoresis is product of the charge q of the particle and the electric field E generated between the two electrodes.

Electrophoresis is the migration of particles under the influence of a direct electric current. There are two requirements for this process to be carried out. First the particles to be separated should be charged or at least should accept charge. Proteins, amino acids, nucleotides etc. conform to this requirement. Second, the medium used for separating the compounds also should carry charge. The mobilities of the ions depend on the nature of the electrolyte solution, the concentration and temperature. The cations and anions move in opposite directions with the cations moving towards the anode and the anions towards the cathode.

Introduction

Gel electrophoresis is a technique for separating charged molecules with different sizes. Two kinds of gels are commonly used: agarose and polyacrylamide. Agarose gels can be applied to a wider range of sizes than polyacrylamide gels.  By using standard agarose electrophoresis, nuclei acids up to 50 kb may be separated. If Pulsed Field Gel Electrophoresis is used, the upper limit can be extended to 10 Mb. Polyacrylamide gels may separate nucleic acids that differ in length by only 1 nucleotide if their length is less than 500 bp (Sambrook et al., 1989).

Principle

The principle of Electrophoresis involves the motion of a particle in a fluid medium, where the gravitational attraction (on a flowing liquid) is the sedimenting force, and the frictional force is the opposing force and is proportional to the velocity of the particle. These forces balance each other acting in opposite directions and hence balance each other. This will lead to the uniform motion of the particle in the mobile liquid medium (i.e. the particle moves at constant velocity). In case the external field is an electric field instead of a gravitational field, a macromolecule will respond to the external field in two ways. In case the molecule is charged, it will migrate in an electric field to the electrode of opposite charge. Under this principle the technique of electrophoresis functions.

All electrophoresis operations are governed by the principle:

Electrophoresis is a method of separating out large molecule ( DNA or protein). An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

Electrophoresis is an important separation technique for the charged molecules. Biologically important molecules, such as amino acids, peptides, proteins nucleotides, nucleic acids which carry electrical charge as they possess certain ionizable functional groups may be separated and identified from a mixture with the help of this technique. Some compounds that are non-polar in nature and do not possess any charge may be transformed to charge carrying derivatives. Thus, phosphate derivatives of carbohydrates may be separated and characterized utilizing electrophoretic techniques.

Introduction 
Building on our extensive exploration of how individual weather parameters intricately influence every facet of crop life and the diverse methodologies employed to evaluate these responses, we now embark on a journey into the mathematical heart of crop science. This chapter, Elementary Crop Growth Models,” will introduce you to the foundational concepts and simpler mathematical frameworks used to quantify, simulate, and ultimately predict how crops grow under varying environmental conditions. In the upcoming sections, we’ll delve into the fundamental building blocks of crop modeling. You’ll discover how these elementary models simplify the complex biological processes occurring within a plant to create manageable, yet insightful, representations. Unlike highly detailed mechanistic models, which require vast amounts of data and computational power, elementary models focus on capturing the most essential relationships between environmental drivers (like solar radiation, temperature, and water) and key aspects of crop growth, such as biomass accumulation or phenological progression. These simpler models serve as a crucial stepping stone. They allow us to grasp core principles of crop-weather interaction in a quantifiable way, providing practical tools for initial assessments, quick predictions, and conceptual understanding, without getting lost in the overwhelming complexity of every single physiological pathway. By understanding these foundational models, you’ll gain a clearer perspective on how agricultural scientists begin to predict the future performance of our food crops, even as the climate around them continues to evolve

Introduction

The basic physiological structures of mammary glands in all species are almost same and are located externally in order to fulfill the milk requirement of neonates after parturition. The process of secretion of milk from the mammary glands is known as lacto genesis. Milk provides an almost complete nutrition to neonates from birth till weaning period. It is a complex mixture of various constituents like:

Lipids such a striacyl glycerols(TAG),phospholipids,cholesterols and other sterols, free fatty acids and fat-soluble vitamins

Carbohydrates such as lactose, oligosaccharides

Proteins such a scaseins, α-lactalbumin, β-lactoglobulin, immunoglobulin’s, bovine serum albumin, lactoferrin, lysozyme, enzymes, hormones and growth factors

Non-protein nitrogenous compounds like urea, creatine, creatinine, uric acid and amino acids

Major and minor minerals, water-soluble vitamins and cells (leucocytes, macrophages, and epithelial cells).

5.1 Elements of Landscape Gardens

5.1.1 Introduction

Elements of a garden are the basic units of any visual design which form its structure and convey visual messages. Elements are based on the art principles of landscaping. Process of landscape design includes concepts and elements of landscape designing. Generally, elements of a garden are divided into 2 types

The plant can absorb more than sixty elements as revealed from the plant tissue analysis, still a few elements is considered essential for the growth and nutrition of the plants. The deficiency of any one of the essential elements can stop completing the life cycle of the plants which is reflected on the yield of the crop. The growth of the plant which is expressed in terms of dry weight, length ,diameter etc. are interlinked with the plant available nutrients of soils.

3.1 Essential Nutrients

The essential elements are those elements which are required for growth and nutrition of the plants. There are 17 elements known to be essential nutrients for plants. The essential elements are classified as micro and macronutrients depending upon the requirement of the crop. Among the naturally occurring elements, eighteen elements are considered essential to plants. These are: C, H, O, N, P, K, Ca, Mg, S, Fe, Mn, Zn, Cu, B, Mo, Cl, Ni, Co. The elements which are found in different sources are required to be in available forms (Table 3.1) for growth and nutrition of the plants.

A. Answer the following.

Q.1.     What are the different phagocytic cells ?
Ans.     The phagocytic cells of mammals belong to two complementary systems (1) The myeloid system consists of cells that are rapidly phagocytic but incapable of sustained effect. (2) Mononuclear phagocytic system act more slowly but capable of repeated phagocytosis.

Q.2.     How 3 types of granulocytes are differentiated ?
Ans.    Three types of granulocytes are distinguished by the staining property of their granules. Cells whose granules take up basic dyes such as haematoxylin (a blue dye) are called basophils, whose granules take up acidic dyes such as eosin (a red dye) are called eosinophils and those that take up neither basic nor acidic dyes are called neutrophils.

Elephant foot yam, also known as suran or jimmikand, is gaining popularity due to its high yield potential and culinary qualities. It is grown in Andhra Pradesh, Bihar, Gujarat, Kerala, and Maharashtra in India, as well as in the north-eastern states of Odisha, Tamilnadu, Uttar Pradesh, and West Bengal. This crop is being embraced for commercial production in northern and eastern India, thanks to the advent of a high yielding, non-acrid variety. Elephant foot yam has an output potential of 50-60 tonnes per hectare. Elephant foot yam is a tropical tuber crop that has been grown for generations in tropical Asia. It is gathered for its edible corms, tubers, and smooth petioles, which all contain important minerals.