eChapters

Electrophoresis is a method of separating out large molecule ( DNA or protein). An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

Electrophoresis is an important separation technique for the charged molecules. Biologically important molecules, such as amino acids, peptides, proteins nucleotides, nucleic acids which carry electrical charge as they possess certain ionizable functional groups may be separated and identified from a mixture with the help of this technique. Some compounds that are non-polar in nature and do not possess any charge may be transformed to charge carrying derivatives. Thus, phosphate derivatives of carbohydrates may be separated and characterized utilizing electrophoretic techniques.

Introduction 
Building on our extensive exploration of how individual weather parameters intricately influence every facet of crop life and the diverse methodologies employed to evaluate these responses, we now embark on a journey into the mathematical heart of crop science. This chapter, Elementary Crop Growth Models,” will introduce you to the foundational concepts and simpler mathematical frameworks used to quantify, simulate, and ultimately predict how crops grow under varying environmental conditions. In the upcoming sections, we’ll delve into the fundamental building blocks of crop modeling. You’ll discover how these elementary models simplify the complex biological processes occurring within a plant to create manageable, yet insightful, representations. Unlike highly detailed mechanistic models, which require vast amounts of data and computational power, elementary models focus on capturing the most essential relationships between environmental drivers (like solar radiation, temperature, and water) and key aspects of crop growth, such as biomass accumulation or phenological progression. These simpler models serve as a crucial stepping stone. They allow us to grasp core principles of crop-weather interaction in a quantifiable way, providing practical tools for initial assessments, quick predictions, and conceptual understanding, without getting lost in the overwhelming complexity of every single physiological pathway. By understanding these foundational models, you’ll gain a clearer perspective on how agricultural scientists begin to predict the future performance of our food crops, even as the climate around them continues to evolve

Introduction

The basic physiological structures of mammary glands in all species are almost same and are located externally in order to fulfill the milk requirement of neonates after parturition. The process of secretion of milk from the mammary glands is known as lacto genesis. Milk provides an almost complete nutrition to neonates from birth till weaning period. It is a complex mixture of various constituents like:

Lipids such a striacyl glycerols(TAG),phospholipids,cholesterols and other sterols, free fatty acids and fat-soluble vitamins

Carbohydrates such as lactose, oligosaccharides

Proteins such a scaseins, α-lactalbumin, β-lactoglobulin, immunoglobulin’s, bovine serum albumin, lactoferrin, lysozyme, enzymes, hormones and growth factors

Non-protein nitrogenous compounds like urea, creatine, creatinine, uric acid and amino acids

Major and minor minerals, water-soluble vitamins and cells (leucocytes, macrophages, and epithelial cells).

5.1 Elements of Landscape Gardens

5.1.1 Introduction

Elements of a garden are the basic units of any visual design which form its structure and convey visual messages. Elements are based on the art principles of landscaping. Process of landscape design includes concepts and elements of landscape designing. Generally, elements of a garden are divided into 2 types

The plant can absorb more than sixty elements as revealed from the plant tissue analysis, still a few elements is considered essential for the growth and nutrition of the plants. The deficiency of any one of the essential elements can stop completing the life cycle of the plants which is reflected on the yield of the crop. The growth of the plant which is expressed in terms of dry weight, length ,diameter etc. are interlinked with the plant available nutrients of soils.

3.1 Essential Nutrients

The essential elements are those elements which are required for growth and nutrition of the plants. There are 17 elements known to be essential nutrients for plants. The essential elements are classified as micro and macronutrients depending upon the requirement of the crop. Among the naturally occurring elements, eighteen elements are considered essential to plants. These are: C, H, O, N, P, K, Ca, Mg, S, Fe, Mn, Zn, Cu, B, Mo, Cl, Ni, Co. The elements which are found in different sources are required to be in available forms (Table 3.1) for growth and nutrition of the plants.

A. Answer the following.

Q.1.     What are the different phagocytic cells ?
Ans.     The phagocytic cells of mammals belong to two complementary systems (1) The myeloid system consists of cells that are rapidly phagocytic but incapable of sustained effect. (2) Mononuclear phagocytic system act more slowly but capable of repeated phagocytosis.

Q.2.     How 3 types of granulocytes are differentiated ?
Ans.    Three types of granulocytes are distinguished by the staining property of their granules. Cells whose granules take up basic dyes such as haematoxylin (a blue dye) are called basophils, whose granules take up acidic dyes such as eosin (a red dye) are called eosinophils and those that take up neither basic nor acidic dyes are called neutrophils.

Elephant foot yam, also known as suran or jimmikand, is gaining popularity due to its high yield potential and culinary qualities. It is grown in Andhra Pradesh, Bihar, Gujarat, Kerala, and Maharashtra in India, as well as in the north-eastern states of Odisha, Tamilnadu, Uttar Pradesh, and West Bengal. This crop is being embraced for commercial production in northern and eastern India, thanks to the advent of a high yielding, non-acrid variety. Elephant foot yam has an output potential of 50-60 tonnes per hectare. Elephant foot yam is a tropical tuber crop that has been grown for generations in tropical Asia. It is gathered for its edible corms, tubers, and smooth petioles, which all contain important minerals.

Elephant foot yam is an underground stem tuber belonging to the family Araceae, one of the most popular tuber crops, extensively used as a favourite vegetable by millions of people in India. It has both nutritional and medicinal value and is usually consumed as cooked vegetable. It has high dry matter production capability per unit area than most of the other vegetables. Elephant foot yam is a remunerative and profitable stem tuber crop. The crop is gaining popularity due to its shade tolerance, easiness in cultivation, high productivity, less incidence of pests and diseases, steady demand and reasonably good price.

5.1 Introduction

In recent decades, human induced changes in climate of the earth have the focus of scientific and social attention. The most imminent of this is the increased concentration of greenhouse gases (GHGs) namely, carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N₂O) in the atmosphere. The concentration of CO₂, CH₄ and N₂O have increased markedly by 30%, 145% and 15%, respectively as a result of human activity since 1750, the era of Industrial Revolutions (IPCC, 2007). Between 1750 and 2005, the concentration of CO₂, CH₄ and N₂O in the atmosphere has increased from 280 to 379 ppm, 715 to 1774 ppb and from 270 to 319 ppb, respectively (IPCC, 2007). However, there was a steep increase during the recent years i.e. 70% increase of GHGs between 1970 and 2004.

Immunoassays rely on the ability of an antibody to recognize and bind a specific macromolecule in what might be a complex mixture of macromolecules. In immunology the particular macromolecule bound by an antibody is referred to as an antigen and the area on an antigen to which the antibody binds is called an epitope.

In some cases an immunoassay may use an antigen to detect for the presence of antibodies, which recognize that antigen, in a solution. In other words, in some immunoassays, the analyte may be an antibody rather than an antigen.

As aquaculture expands rapidly across states like Andhra Pradesh, Tamil Nadu, Kerala, and West Bengal, the demand for accurate and timely disease detection methods has intensified. Enzyme-Linked Immunosorbent Assay (ELISA) and other serological tools have emerged as critical assets in pathogen surveillance and management in fish and shrimp farming systems.
ELISA, widely used in Indian diagnostics laboratories, enables the detection of pathogen-specific antigens or host antibodies with high sensitivity and throughput. Its capacity to analyze multiple samples simultaneously and deliver quantitative results makes it particularly suitable for large-scale disease monitoring. Both indirect and sandwich ELISA formats are employed, tailored to the diagnostic goal—whether it is detecting the pathogen itself or assessing the host’s immune response.
In indirect ELISA, fish serum samples are screened for antibodies against key pathogens. This format is instrumental in vaccine efficacy studies and epidemiological surveys. For instance, aquaculture centers in Kerala and Tamil Nadu use indirect ELISA to track immune responses to Aeromonas hydrophila and Koi Herpesvirus (KHV) in ornamental and carp species. On the other hand, sandwich ELISA is utilized for direct antigen detection in tissues and fluids, and is applied in shrimp hatcheries for diseases such as White Spot Syndrome Virus (WSSV) and Yellow Head Virus (YHV), supported by ICAR and MPEDA programs.
Other serological techniques also find relevance in Indian aquaculture. Agglutination assays, especially slide agglutination, offer rapid and cost-effective field diagnostics. These are applied in the detection of Edwardsiella tarda and Streptococcus iniae in tilapia and catfish. Latex agglutination further enhances rapid bacterial identification at the farm or lab level. Immunodiffusion techniques, such as Ouchterlony double diffusion, though less sensitive, are still used in resource-limited areas for detecting fungal pathogens like Ichthyophonus and Saprolegnia.

Teaching and research in anatomy is mainly based on cadaver dissections. A sound knowledge of anatomy is essential from the beginning of veterinary education and the knowledge obtained through dissection of animal body is an indispensable part of the veterinary science.
History of embalming
Embalming is the art and science of preserving the animal by treating with chemicals to forestall decomposition. Embalming destroys the colloid nature of the proteins and neutralizes the active centres of the molecules forming many cross linkages of adjacent protein molecules. Proteins are thus converted to high molecular inert solid materials that can no longer serve as food for bacteria or as a substrate for enzyme action. Embalming preserves the body from decay. The embalmed bodies are stored in tanks containing a fluid composed of formalin, phenol, thymol and rock salt in water in varying proportions.
Claudius Galen (130-200 A.D) did dissections of animals such as the pig or monkey. Leonardo Da Vinci (1452-1519) a pioneer anatomist introduced anatomical drawings. During 15th-16th century the motive was to advance the techniques for the preservation of the dead and to study detailed anatomy by dissections. Andreas Vesalius (1514-1564) believed that dissection was necessary to find out how bodies worked. His lectures were based purely on his observations. He carried out dissection on human cadavers as well as animals to understand Anatomy. As an Anatomist he represents the end of the middle ages obscurantism and the beginning of modern Anatomy and scientific thinking. He is considered the father of modern Anatomy. In 17th century Antonie Van Leeuwenhoek (1632-1723) opened up new horizons

8.1 Design of Canal Embankments

 There are two aspects of canal embankment design.

 (i) The general requirement, such as free board, dowel, service/inspection road, drainage of banks etc. The aspect of free board is discussed in Para

8.2 to 8.3 and that of service/ inspection road in Para

 8.4 and drainage of banks in Para 8.5 in this chapter.

 (ii) Safe design of embankment i.e. Hydraulic and structural requirement.

It includes stability of inner and outer slopes, Structural safe design of inner cut slopes in deep cuttings in different types of soils/rocks of varying nature and ground water conditions. Stability of slopes may include drainage, so as to prevent rock falls/debris in canal. For outer slopes in embankment (filling sections) hydraulic gradient/pharetic line, toe drains etc, similar to the earthen dams, on different types of soil/rock foundation with respect to varying ground water level conditions are important.

The canal embankments in filling may even fail through its foundations by sloughing or liquefactions. All these aspects of canal embankments are discussed in this chapter in Para 8.4 to 8.9.

I. FILL UPS

1. The developing ovule is commonly attached to the placenta by the _____.
2. The scar on the ovule made where the funiculus detaches at maturity is known as the _____.
3. The point where the integuments meet at the nuclear apex is _____.
4. Opposite to the micropyle, the region of integumentary origin and attachment is called _____.
5. Between the chalaza and the hilum is an area called _____.
6. _____ is an example for species with developed perisperm.

Introduction
The urinary system comprises of two kidneys, two ureters, a urinary bladder, and a urethra) The latter is shared by the genital organs.
• The urinary system develops from the intermediate mesoderm
• Three different kidney systems are formed in vertebrates--pronephros, mesonephros, and metanephros.
Pronephros: - Forms a duct that empties into the cloaca- Pronephric tubules connect each body segment with the coelom (body cavity) Pronephric tubules never become functional in mammals
Mesonephros: - Pronephric duct becomes mesonephric duct Distal to the pronephric tubules appear mesonephric tubules that surround tufts of blood vessels (glomeruli), but no loop of Henle is formed Blood is transported to mesonephric tubules, filtrate is transported to the mesonephric duct and finally to the urogenital portion of the cloaca
Metanephros: - Metanephric bud (ureteric bud) Metanephric blastema
• Bud gives rise to the ureter, the pelvis of the kidney, and collecting tubules.
• Blastema gives rise to nephrons.
The internal structure of the kidney varies among species. It is determined by the number and size of calyces which branch from the renal pelvis. Each calyx forms the base of a renal lobe (renal pyramid). All mammalian kidneys begin as multipyramidal (multilobular) structures, but lobules fuse to varying degrees, depending upon the species. (Note, this multilobulation is maintained in the histological structures)

Bone is the only tissue in the body able to heal without microscopical scarring. On the other hand fracture healing is a slow process causing long immobilization periods with consequently high costs for fracture healing giving rise to delayed healing, fibrous healing or non-healing with subsequent problems for both, the patient and the orthopedic surgeon. Fracture healing is complex phenomenon which involves numerous cells, regulators of cell function and biochemical interactions in the repair process. The healing potential of bone, in a fracture model is influenced by a variety of biochemical, biomechanical, cellular, hormonal and pathological mechanisms. A continuous occurring state of bone deposition, resorption and remodeling facilitates the healing process.

Biodegradable and bioinert ceramic materials such as hydroxyapatite (HA), tri-calcium phosphate (TCP), aluminum-calcium-phosphate (ALCAP) ceramics provides scaffold to support the attachment and migration of newly formed bone cells into the osseous defect and also help in formation of a vascular network through the newly formed bone. Bioceramic also act as a carrier to deliver stem cells for osteogenesis.

Specific bone-inductive proteins/growth factors can induce bone formation and healing in vivo. These morphogens are therefore, to be an ideal alternative to autogenous bone grafts. These findings initiating intensive research into bone regeneration orchestrated by putative soluble signals and lead to the discovery and identification of an entirely novel family of protein initiators collectively called the bone morphogenic protein (BMPs), which belong to transforming growth factors-ß (TGF- ß) super family. These growth factors might possess a biological activity that is site and tissue specific in the induction of bone formation. Different growth factors have been demonstrated to possess osteoinductive and fracture healing properties.

Stem cells have therapeutic potential in the realm of orthopaedic surgery because of their capacity to self-renew and differentiate into various types of mature cells and tissues, including bone. Embryonic stem cells (ESCs) are pluripotent cells derived from the inner cell mass of pre-implantation embryos and represent embryonic precursor cells that give rise to any cell type in the embryo. Specifically, it has been shown that ES cells can differentiate into osteogenic cells under selective culture conditions. These osteogenic cells are capable of in vitro producing bone nodules in three dimensional scaffolds.

1. INTRODUCTION

Until the 1990s, the phrase Corporate Governance (CG) was relatively unknown but since then it has gained a huge importance when the corporate sector was surrounded with problems of unethical practicesand flagrant violations of rules and regulations in a number of countries. CG has received increased attention as multiple cases of risk of corporate failures both for public and private sectors. Multiple corporate failures worldwide, such as those of Maxwell, Enron, Global crossing, and Sunbeam forced the regulatory authorities to set up codes and standards on CG.The Indian market has also faced a number of scams since early nineties.Harshad Mehta Stock Scam in 1992 is one of the corporate frauds in the country which finally resulted in setting up of Kumar Mangalam Birla Committee in 1999. Based on their recommendations in 2000, Clause 49 on CG was inserted in the listing agreement of every stock exchange in India. The purpose was to promote and raise standards of CG of listed companies. CG has now become most common phrase in the business world of 21^st century. It is important for financial health of the corporations and relationshipwith the public at large; may it be an individual or community especially after the collapse of Satyam Software Services Ltd. in 2008.It has been made mandatory for the Indian companies to comply with CG norms, to give reports and disclosures as per the listing agreement and various provisions of the Companies Act in their annual reports. The importance ofCG in the 21^st century keeps on increasing as the economy is growing.

Introduction

Electronic resources represent an increasingly important component of the library collection building. “Electronic resources” refer to those materials that require computer access, whether through a personal computer, mainframe, or handheld mobile device. They may either be accessed remotely via the Internet or locally. Some of the most frequently encountered types are:

1 In emergency medicine, first aid measures do not include examination of

a) Airway b) Breathing

c) Circulation d) Blood Pressure

Emergency medicine deals with patients those are critically ill and requires immediate healthcare for survival. Every life threatening disease is presented
for emergency management and the clinician is required during emergenices to successfully manage these with strong working knowledge, quick assessment
and appropriate action.

Emerging preservation technologies are being developed to meet increasing expectations for food safety, nutritional quality, sustainability and longer shelf life. Greater investigation remains necessary to understand their effects, limitations and suitability for large-scale industrial application. This chapter examines innovative physical, chemical and biological methods used at different stages of the food chain. Technologies include plasma-activated water, cold atmospheric plasma, advanced oxidation, electrolysed water, space-food preservation, hurdle systems and biopreservation. Their mechanisms involve reactive species, microbial membrane damage, oxidation, combined environmental stresses and beneficial biological agents. The chapter assesses their applications, advantages and disadvantages while highlighting research, regulation, process standardisation, equipment cost and commercial scalability.

Emerging Infectious Diseases
Emerging infectious diseases are those due to newly identified and previously unknown infections which cause public health problems either locally or internationally.
Re-emerging Infectious Diseases
Re-emerging infectious diseases are those due to the reappearance and increase of infections which are known, but had formerly fallen to levels so low that they were no longer considered a public health problem.
What Causes Emergence or Re-emergence of Infectious Diseases?
Several factors contribute to the emergence and re-emergence of infectious diseases, but most can be linked with the increasing number of people living and moving on earth: rapid and intense international travel; overcrowding in cities with poor sanitation; changes in handling and processing of large quantities of food; and increased exposure of humans to disease vectors and reservoirs in nature. Other factors include a deteriorating public health infrastructure which is unable to cope with population demands, and the emergence of resistance to antibiotics linked to their increased misuse.
Avian Influenza
Avian influenza is a zoonotic, globally important disease of birds that can be categorised as either low pathogenic (LPAI) or highly pathogenic (HPAI) according to the virulence of the virus in animals. Outbreaks of LPAI are common around the world but LPAI typically causes no clinical signs or only minor illness in infected birds. LPAI strains are generally less of a threat to human health; in addition, LPAI can have significant economic repercussions as a result of export restrictions and culling of birds, particularly in developing countries where the use of vaccines and other veterinary medicines is difficult
due to weak veterinarian services and small farming units. Compared with LPAI, HPAI is more readily detected in poultry due to very high levels of mortality in infected birds, and can cause potentially catastrophic economic consequences, from significantly reduced livestock populations to lost export markets. Though relatively rare, sporadic human infections of HPAI have occurred in cases of close contact with infected birds and caused serious illness
and even death.

Emerging Infectious Diseases
Emerging infectious diseases are those due to newly identified and previously unknown infections which cause public health problems either locally or internationally.
Re-emerging Infectious Diseases
Re-emerging infectious diseases are those due to the reappearance and increase of infections which are known, but had formerly fallen to levels so low that they were no longer considered a public health problem.
What Causes Emergence or Re-emergence of Infectious Diseases?
Several factors contribute to the emergence and re-emergence of infectious diseases, but most can be linked with the increasing number of people living and moving on earth: rapid and intense international travel; overcrowding in cities with poor sanitation; changes in handling and processing of large quantities of food; and increased exposure of humans to disease vectors and reservoirs in nature. Other factors include a deteriorating public health infrastructure which is unable to cope with population demands, and the emergence of resistance to antibiotics linked to their increased misuse.

Introduction

The antibiotics are low molecular weight natural, semi-synthetic, or synthetic substance that kills or slows the growth of germs while causing little or no harm to the host is considered an antimicrobial (Walsh, 2000). Antibiotics are the type of medicine produced by some microorganisms that kill or slow the growth of other microorganisms. It includes any of a large group of chemical substances, as penicillin or streptomycin, produced by various microorganisms and fungi, having the capacity in dilute solutions to inhibit the growth of or to destroy bacteria and other microorganisms, used chiefly in the treatment of infectious diseases (De Vetten et al., 2003). However today, a large number of bacteria are resistant to various antibiotics. Antibiotic resistance occurs when a bacterium becomes able to survive and multiply despite the presence of an antibiotic that would normally control or kill it. In other words, the bacteria become “resistant” and continue to grow even at therapeutic levels of the antibiotic. While antibiotic resistance is a natural phenomenon, it is accelerated by the selective pressure exerted when an antibiotic is used. Bacteria that are naturally resistant to the antibiotic have a better chance of survival compared to those that are susceptible. As the susceptible bacteria are killed or inhibited, resistant strains are favored and continue to thrive (Holme et al., 2006). Some resistance arises without human influence, as bacteria can naturally produce antibiotics against other microorganisms, resulting in a low-level, natural selection for antibiotic resistance. However, the current surge in antibiotic resistant bacteria is largely attributed to the overuse and misuse of antibiotics. One of the main issues facing public health is antimicrobial resistance, particularly in developing nations where access to and use of medications are easier than in developed nations, leading to a disproportionately higher rate of inappropriate antibiotic use and higher resistance levels (Ganguly et al., 2011). Antimicrobial resistance will make it more difficult to control infections in the community and make health care services less effective (Bhatia et al., 2010). It is well known that India has one of the highest rates of infectious disease burdens in the world, and that the country’s high rates of malnutrition and inadequate sanitation make these circumstances worse (http://www. resistancestrategies.org/wpcontent/uploads/2011/03/Indiareport).

1 INTRODUCTION

Aquaculture has been growing at higher rate than any other animal food-producing sector globally with Asia dominating the production. Considering the higher consumer demand for aquatic animal food, coupled with stagnating harvest from capture fisheries the rapid growth of aquaculture is expected to continue in the future. For instance, the global production of farmed food fish including finfishes, crustaceans, molluscs, amphibians (frogs), aquatic reptiles (except crocodiles) and other aquatic animals such as sea cucumbers, sea urchins, sea squirts and jellyfishes was 59.9 million metric tones (mmt), up by 7.5 percent from 55.7 mmt in 2009 (FAO, 2012). Asia accounted for 89 percent of world aquaculture production by volume in 2010 with China alone accounting for more than 60 percent of global aquaculture production volume in 2010. The other major producers in Asia are India (4.65 mmt), Viet Nam (2.67 mmt), Indonesia (2.30 mmt), Bangladesh (1.31mmt), Thailand (1.29 mmt), Myanmar (0.85mmt), the Philippines (0.74 mmt) and Japan (0.72 mmt). Further, in light of increasing population pressure on land, intensification of aquaculture practices has been taking place and now two-third of all food fish production aquaculture is achieved with artificial feeding (FAO, 2012). In recent years it also has become apparent that aquaculture production is vulnerable to adverse impacts of diseases and environmental conditions as farmed organisms are susceptible to a wide range of factors that can pose a major threat to a thriving aquaculture industry with considerable economical repercus-sions. For instance, in 2010, aquaculture in China suffered production losses of 1.7 million tonnes caused by natural disasters, diseases and pollution. Disease outbreaks virtually wiped out marine shrimp farming production in Mozambique in 2011 (FAO, 2012). In addition, impacts of culture environment, and harvest and post-harvest handlings on flesh quality are also increasingly becoming important issues.

Technology Scouting and Innovations in Technology Incubation
Technology scouting and innovations in technology incubation have a symbiotic relationship, fuelling each other for the benefit of budding startups and the broader technology ecosystem. Let’s dive deeper into their individual roles and how they interact:
Technology Scouting
Technology Scouting is the process of discovering, analyzing and evaluating new or existing technologies that will help them with their innovation process. In simple terms, it is a process for companies to find the necessary technology outside the company.
Hunting for promising technologies: Scouts actively search for emerging technologies, patents, research advancements, and market trends across various sectors.
Evaluating potential and fit: They assess the technical feasibility, market potential, and compatibility with the needs of incubators and their supported startups.
Connecting innovators and ventures: Scouts bridge the gap between technology creators (researchers, inventors, startups) and potential users (incubators, established companies).
Fuelling innovation pipelines: Technology scouting helps incubators access a wider range of technologies and ideas, enriching their portfolio and diversifying the innovations supported.

Abstract
Emerging contaminants (ECs) are a class of newly discovered contaminants that have been identified as major water pollutants that have negative impacts on human health in recent years. These micropollutants are said to bioaccumulate in people, other aquatic food web creatures, and macro invertebrates. Conventional treatment methods are unable to eliminatethem. Only advancements in analytical  instrumentation made it possible to identify these pollutants, even at minuscule amounts. As new compounds are discovered over time, concerns are raised about their origins, fate, transportation, transformations, and effects on aquatic habitats. The purpose of this chapter is to provide an overview of the contaminants that are categorized as “emerging,” together with information on their sources, entry points into the environment, and associated threats to environment and possible treatments to somehow eliminate them.
Keywords: Emerging contaminants, Micropollutants and Aquatic System.

1. INTRODUCTION

Science and Technology are vital for developing low-input and high-output agriculture and pharmaceutical. India needs frugal use of natural resources and diversification of agricultural activities. Transgenic technologies are highly misunderstood. Genetically modified crops are a result of them. In fact genetic modification of crops for human use is going on since their domestication. By introduction of Bt cotton in 2002, yields have doubled. American bollworm can no longer threaten Indian cotton crops. Insects can develop resistance to Bt gene or some virus attacking the crop needs more research. RNA-mediated gene suppression may aid the development of patent strategies that will support the next generation of genetically modified crops and enhance scope of patenting. Many fancy Organic farming, which will need 40 percent more agricultural land for present number of people on earth although it is ever increasing. Biotechnology has revolutionized agriculture, healthcare, industrial processing, environmental sustainability and is also a revenue generator. India is poised for a tremendous growth in BT sector. NanoBT has now entered into furthering the useful research on faster pace. It has scientific and technical pool of human resources, rich biodiversity, large agricultural and market base for new BT products and world recognition as a producer of low cost, high quality bulk drugs and formulations. Issues surrounding Intellectual Property Rights and BT inventions are varied but interrelated. Basic research has to be transferred to applied field and the resultant is to be commercialized. In some countries, plant varieties are protected by sui generis system. Post genome research has added fuel to the fire; new innovative fields, like ‘Bioinformatics’ with plenty of biology information and new processing methods have emerged. BT uses four tools of IPR, viz. Patents, Trademarks, Trade secrets and Copyright. At present, its contribution to the World’s patenting activity is less than three percent. As such there is a necessity to amend the IP laws to give better protection to R&D, and better enforcement of IP, technology transfer and licensing arrangements. Biotech industry in India is flourishing more in pharmaceuticals, particularly in healthcare. There is an urgent need for harmonization of administration and centralization of the patenting process in accordance with WIPO diktats.

Introduction

Food fraud is not new it can be traced back to ancient Rome and Athens, where regulations prohibiting the adulteration of beverages with flavours and colours were in place. It exists since the food trade has been started. Food fraud affects all food supply chains, thus the entire food sector. Due to globalisation and the increasing complexity of supply lines, food supply chains are becoming more vulnerable to food fraud (Robson et al., 2021a). A fraud is “any act or omission relating to the use or presentation of false, incorrect, or incomplete statements or documents, or non disclosure of information in violation of a specific obligation, and any other illegal activity affecting the financial interests” (EU No. 2017/1371).

Introduction
In the paradigm of development, the Government of India generated a Central Sector Scheme for the people associated with agriculture – a sector contributing to 18.2 per cent of the nation’s GDP, titled “Formation and Promotion of 10,000 Farmer Produce Organizations (FPOs)” with major focus on upheaval of farming community. With well-defined strategy and committed resources the Scheme is set-out to formulate 10,000 new FPOs in the country with budgetary provision of Rs 6865 crore. At the core of formation of each FPO, that are to be developed in produce clusters, wherein agricultural and horticultural produces are grown / cultivated for leveraging economies of scale and improving market access for members. “One District-One Product” cluster to promote specialization and better processing, marketing, branding & export. Under this Central Sector Scheme with funding from Government of India, Formation & Promotion of FPOs are to be done through the Implementing Agencies (IAs). Presently 09 Implementing Agencies (IAs) have been finalized for formation and promotion of FPOs viz. Small Farmers Agri-Business Consortium (SFAC), National Cooperative Development Corporation (NCDC), National Bank for Agriculture and Rural Development (NABARD), National Agricultural Cooperative Marketing Federation of India (NAFED), North Eastern Regional Agricultural Marketing Corporation Limited (NERAMAC), Tamil Nadu-Small Farmers Agri-Business Consortium (TN-SFAC), Small Farmers Agri-Business Consortium Haryana (SFACH), Watershed Development Department (WDD)- Karnataka, and Foundation for Development of Rural Value Chains (FDRVC)- Ministry of Rural Development (MoRD). The Implementing Agencies (IAs) engage Cluster Based Business Organizations (CBBOs) to aggregate, register & provide professional handholding support to each FPO for a period of 5 years. CBBOs have been empanelled & engaged by IAs. CBBOs operate as an end-to-end knowledge for all issues related to FPO promotion. During 2020-21, a total of 2200 FPO produce clusters were allocated for formation of FPOs, which also include specialized FPO produce clusters such as 100 FPOs for Organic, 100 FPOs for Oil seeds, etc. Of these, 369 FPOs are targeted for 115 aspirational districts in the country. Additionally, NAFED would form the specialized FPOs which should necessarily be forwardly linked to the market, agri-value chain, etc. NAFED will provide market and value chain linkages to the FPOs formed by other Implementing Agencies. NAFED has formed & registered 05 Honey FPOs during current year in Uttar Pradesh, Madhya Pradesh, Rajasthan, Bihar and West Bengal. FPOs are earmarked to provide financial assistance upto Rs 18.00 lakh per FPO for a period of 03 years. In addition to this, provision has been made for matching equity grant upto Rs. 2,000 per farmer member of FPO with a limit of Rs. 15.00 lakh per FPO and a credit guarantee facility upto Rs. 2 crore of project loan per FPO from eligible lending institution to ensure institutional credit accessibility to FPOs.

The Asia-Pacific region, home to a large proportion of the world’s agricultural producers and consumers, faces complex challenges: climate change, infrastructure gaps, market fragmentation, and inefficient supply chains that result in high post-harvest losses and limited farmer incomes. However, the region is witnessing rapid innovation across multiple fronts that promise to transform agri-food supply chains into more climate-resilient, transparent, inclusive, and efficient systems. These emerging opportunities align with regional sustainability goals and create pathways for economic growth and rural development. 7.1 Climate-Smart Infrastructure: Financing Low-Carbon and Resilient Agri Supply Chains Green Bonds for Solar Cold Rooms Vietnam’s Mekong Delta — a critical rice-producing region — is pioneering the issuance of green bonds to finance solar-powered cold rooms under the “One Million Hectares” low-carbon rice initiative

Introduction

Pedagogy in higher education is often referred to as activities of educating, instructing or teaching to impart knowledge and skills (Diaz, 2013). With the onset of the Covid-19 pandemic in the year 2020, instructors worldwide faced a challenge in maintaining the teaching pedagogy suited to the online teaching environments. Many instructors with no prior experience teaching online started developing new approaches to solve the challenges in a changing landscape. The main challenges to solve was concerning creating favourable learning experiences, creating engaging classroom experiences, and meeting the expectations of all the students in the online environment (Teachonline, 2020). The internet has provided a wide range of opportunities, leading to uninterrupted education through various conferencing platforms. Since the year 2000, the proliferation of Web 2.0 was tested in the field of education with several tools such as blogs, wiki articles and use of social media channels. Web 2.0 provided increasing scope to the teachers and students to create, edit and share content which is appealing to the learners. Currently, we have entered the Web 3.0 era in which the technologies can be customized to enhance the learning environment for better facilitation of learning needs.

Introduction

Humans have inhabited the earth for nearly 2 million years. For over 90 per cent of this period, the maximum population numbered about 200,000 and the growth rate was only about 0.01 percent per year. The use of fire and the development of tools were the first major changes affecting human population growth which increased to about 5 million individuals about 100,000 years ago. The population remained fluctuating about this mean value for the next 90,000 years depending upon the food supply. Up to this time people were hunterer-gatherers and depended exclusively on the natural ecosystem for their food. Then about 10,000 years ago, humans began to cultivate crops for food. With established agriculture came an increased and stable food supply. This contributed to an increased growth rate of the human population (Dhaliwal et al., 1998).

2.1    Introduction

The hot and usually wet conditions in tropics adversely affect the performance of insecticides as well as biology of the pests. Pesticide usuage is declining throughout the world, but in tropics it is likely to increase threatening basic biological components of pest management, environment and safety of people.  Pest management in tropical agriculture is basically influenced by high diversity of organisms, diversity of physical features and ecosystems, moderate seasonal weather changes, and the human factor. The high biological diversity of the tropics includes a wide range of interacting organisms from which have been identified pests that affect health crops and livestock as well as cultural practices for pest management.  Physical features of the tropics are also diverse, and include high-altitude temperate zones, rain forests, savannah woodlands, grasslands, and desert zones. Each zone has its own biological diversity either existing in isolation or overlapping with others. Moderate seasonal weather changes in the tropics affect populations indirectly, through modification of interactions between organisms.

Pest resurgence is defined as the rapid reappearance of a pest population in injurious numbers following pesticide application. Use of persistent and broad spectrum pesticides that kills the beneficial natural enemies is thought to be the leading cause of pest resurgence. However, resurgence is known to occur due to several reasons, for instance, increase in feeding and reproductive rates of insect pests, due to application of sub lethal doses of pesticides and sometimes elimination of a primary pest provides favorable conditions for the secondary pest to become primary pests (Harsh Garg, 2014). There are several pesticide induced pest outbreaks reported in walnut, hemlock, cotton and soybean. Among these, brown plant hopper in rice cultivation has gained a major importance in Asian countries. In general, natural BPH populations were kept under check by natural enemies including mired bugs, ladybird beetles, spiders and other pathogens. Pesticides, however, not only have destroyed the natural enemies, but have influenced the fecun

1. Introduction
In recent decades, environmental contamination has emerged as a seriousthreat to our modern society. The relentless growth of urbanization andindustrialization leads to the ever-increasing production of hazardouschemicals. Safe disposal of these hazardous materials is crucial, as releasingthem into an already strained environment has severe consequences. Theexponential rise in human activity is directly related to the alarming risein local and global pollution levels, which severely disturbs the balance ofecosystems. Industrial activities, in particular, are a major source of highlyhazardous chemicals such as dyes, pigments, and aromatic compounds,which pose a serious threat to the environment. In India, the textile industry, amajor force driving the country’s economic growth, also ranks asthsecondlargestpolluter. The printing, finishing, and dyeing processes used in textileproduction generate significant waste (Dubey et al., 2018). Dyes, a majorsource of pollution in this industry, are syntheticand complex in structure. Thiscomplexity makes them poorly soluble in water, hindering biodegradation andposing a long-term environmental concern. The global textile industry utilizesa wide range of dyes, with estimates indicating over 100,000 commerciallyavailable options. The worldwide production of these dyes is estimated to be700,000 tons per year (Al-Tohamy et al., 2020). However, dye contaminationis a major challenge in this industry. Studies indicate that during synthesisand dyeing processes, a significant portion, ranging from 10% to 15% of thetotal dyestuffs used ends up released into global water bodies (Leal et al.,2020). A significant portion of textile dyes, ranging from 2% to 20%, aredirectly released into water reservoirs through wastewater effluents. This is

1.1 Introduction Rural women worldwide grapple with many challenges, ranging from restricted access to essential resources and limited educational opportunities to constrained pathways for economic advancement. Extensive research conducted by the World Bank has illuminated the persistent struggles faced by rural women, emphasizing disparities in education, healthcare, and economic opportunities (World Bank, 2019). Simultaneously, UN Women underscores the systemic hurdles perpetuating gender inequalities, necessitating urgent and comprehensive solutions (UN Women, 2020).

Amidst these challenges, emerging technologies stand as a transformative force with the potential to reshape the trajectories of rural women. Recognizing this potential, the International Telecommunication Union (ITU) emphasizes the capacity of technological interventions to ameliorate challenges faced by women in rural areas.

Introduction

Foods deteriorate in quality due to a wide range of reactions that may be physical, chemical or biological. The various forms of spoilage and food poisoning caused by micro-organisms are preventable to a large degree by a number of preservation techniques, most of which act by preventing or slowing microbial growth. These include freezing, chilling, drying, curing, conserving, vacuum packing, modified atmosphere packing, acidifying, fermenting, and adding preservatives. Thermal processes such as blanching, pasteurization or heat sterilization have long been employed in practice as economic, efficient, reliable and safe food preservation methods.

7.1 Introduction

Sustainable agricultural systems must be adopted to increase the food production keeping in view of increase in human population. In tropical countries, insect pests are major challenges to increase food production by causing significant damage in the crop fields and stored grains and their products. Interventions by implementing timely integrated pest management strategies are necessary to reduce the yield losses. Efforts to control insect pests have predominantly relied on the use of broad-spectrum insecticides.  However, indiscriminate and irrational applications of insecticides have created the problems such as insecticide resistance, resurgence, secondary pest outbreak and the presence of residues in food and environment (Ansari et al., 2013). Globally, to reduce the pesticide dependence, various technologies have been developed using different approaches to monitor and maage insect pests which are discussed in this chapter.

Abstract
Emerging technologies are reshaping agriculture and revolutionizing food production systems. This chapter explores the latest technological advancements in agriculture, including precision farming, artificial
intelligence, biotechnology, robotics, and digital platforms. It examines how these technologies are being implemented to address the challenges of modern agriculture, such as increasing productivity, enhancing sustainability, and ensuring food security. The chapter also discusses the future directions of food production, considering the impact of technological innovations on global food systems, resource management, and environmental sustainability. By analyzing current trends, opportunities, and potential challenges, the chapter provides a comprehensive overview of how emerging technologies are poised to transform agriculture and the future of food production.
Keywords: Emerging technologies, agriculture, food production, precision farming, artificial intelligence, biotechnology, robotics, digital platforms, sustainability, food security.

Technological advancements in aquafeed production are significantly reshaping the aquaculture landscape by enhancing feed utilization, sustainability, and nutritional performance while cutting costs and reducing ecological impact. One major innovation is precision feed formulation through artificial intelligence (AI) and machine learning, enabling species-specific diets tailored to growth stages and environmental conditions. This customized approach improves nutrient absorption, reduces waste, and boosts farm profitability. Modern processing methods such as extrusion and cold pelleting have further improved pellet durability, digestibility, and water stability—essential for use in systems like Recirculating Aquaculture Systems (RAS) and offshore cages. The transition from traditional feed ingredients to alternatives like insect meal, microbial biomass, algae, and single-cell proteins is also gaining momentum. These sustainable inputs not only offer high-quality nutrition but also lower the environmental burden associated with fishmeal and soy.

1. INTRODUCTION

The growing demand of consumers for fresh, safe, chemical, additive or preservative free and minimally processed foods has compelled the food scientists and technologists to develop many non-thermal food processing technologies which cause minimum nutritional and sensory qualities impairment in the processed foods. In this endeavour, consumers prefer minimally or non-thermally processed foods rather than the thermally processed one. Today, non-thermal technologies like High Pulse Electric Field (HPEF) processing, High Hydrostatic Pressure (HHP) processing or Hight Pressure Processing (HPP) food irradiation, ultraviolet light, ultra sound, arc discharge, oscillating magnetic fields, light pulses, plasma, chemicals (ozone, carbon dioxide, argon), and the combined methods of these technologies are the major areas of research in the process and food engineering in order to find their suitability for processing of various food products.

Abstract

Consumers are now more conscious about quality and source of their foods. Attempts made to determine the quality of food materials are numerous, but most of them are destructive in nature. In recent years, nondestructive methods of quality evaluation have gained momentum and a considerable attempts have been made to develop them. This paper describes application of these non-emerging technologies: magnetic resonance imaging, x-ray computer tomography, near infrared spectroscopy and a few other important techniques for determination of different quality attributes with special emphasis on grain, and discusses their pros and cons for practical exploitation.

Abstract

India ranks first among world’s mango producing countries accounting for about 50 % of the world’s total mango production. In Bihar it is the most important fruit crop as well as occupies a large area affecting socio economic conditions of the region. The crop suffers from over 140 diseases all over the world. From young seedlings to mature trees and fruits in their all stages from start to storage may suffer from a number of biotic and abiotic stresses. All parts of the plant namely, trunk, branch, twigs, leaf, petiole, flower and fruits are prone to attack by a number of pathogens The changes in climatic patterns and shifting towards commercial cultivation of this crop has seen the emergence of some diseases which can cause huge losses to the production of mango over the years. One such problem, decline in mango is reported to be a complex problem involving multitude of factors. Decline disorders are recognized in virtually all mango-producing regions of the world. In most cases, the disease has been characterized by leaf drooping and drying; bark splitting, gum secretion from branches and main trunk, bud necrosis, dieback, vascular discoloration and death of the whole tree. The bark beetle Hypocryphalus mangiferae is closely associated with trees affected by mango sudden decline disease. Report suggests the causal agent to be Botryodiplodia theobromae, Ceratocystis manginecans, Fusarium spp, Colletotrichum sp, Pestalosiopsis maniferae. In most of the cases Botryodiplodia theobromae, Ceratocystis spp are associated with it. Although, none of the pathogens isolated could singly incite the complete decline symptoms under artificial inoculation on mango seedlings indicating towards complex etiology of the disease. Since pathogens are endophytic in nature; mangement of the pathogen after infection is difficult and relies more upon prohytalctic measures. Over all plant health, its nutritional status and abiotic conditions of flood and drought are also the pre disposing factors to decline in Mango. Effective management of decline in mango needs holistic approaches using optimum nutrition, general sanitation, routine crop protection schedule, weed management and appropriate intercropping practices.

Intelligent healthcare is advancing through the integration of multimodal data, trustworthy AI, federated learning, digital twins, IoT devices and real-time decision-support systems. This chapter reviews the evolution of healthcare-data analysis and examines emerging methods for intelligent diagnosis and predictive care. It discusses explainability, privacy-preserving analytics, interoperability, data governance and ethical frameworks necessary for responsible implementation. Wearable technologies and connected sensors support continuous monitoring, while human–AI collaboration improves clinical decision-making. Case studies illustrate practical healthcare applications and implementation strategies. The chapter also considers global-health requirements, particularly in developing countries, and identifies future opportunities for scalable, equitable, secure and clinically effective intelligent healthcare systems.