eChapters

ABSTRACT

Plant tissue culture is an important and efficient technique for the production of desired plant products with different and improved variety in aseptic conditions. Plant tissue culture methods have a wide scope for the creation, conservation, and utilization of genetic variability for the genetic improvement of field, fruit, vegetable, forest crops, medicinal and aromatic plants. The main objective of this communication were to collects the information and techniques of tissue culture to genetic improvement of the medicinal plants. Tissue culture technique in combination with molecular techniques has been successively used to incorporate specific traits through gene transfer. Modified varieties of medicinal plants is mostly required, as some compounds like alkaloids, terpenoids, steroids, saponins, phenolics, flavanoids, and amino acids etc are extracted from these plants. From the ancient time up to 20th century, most of the human-being are dependent on the medicinal plant for basic healthcare needs and improving such a crop is the most important for this billionaire world. As far conventional methods of genetic improvements the tissue culture techniques takes cheap and lesser time required for traits specific improvements in the MAPs.

ABSTRACT

Plants are the basic requirement upon which all other living organisms depend. Plant resources have been used over the decades for human welfare in promotion of health as medicine, flavour and fragrance. Natural essential oils derived from plants are used as cosmetics, perfumes and flavors in foods and beverages. Development of processsing industries are directly linked to specific needs, available resources and intervention of technological capabilities. Resourgence of public interest in herbal products has created a huge market for plant based products which not only satisfies human needs, but also provides quality and safety insurance. Himalaya range in Southeast Asia is a hub of medicinal and aroma bearing plants globally recognized for their chemical constituents as well as endemic and threatened nature. These plant resources has high demand in international market for development of value added products, for isolation of commercial important compounds used in drug discovery programmes, preparation and formulation for development of new nutraceutical products and semi-synthetic derivatives for medicines. Aromatic plants are valued for their aromas, tastes and their applications in treatment of various illness, and mostly prefered in cosmatics, perfumes, confectionery food items and medicines. More than 3000 aroma bearing plants have been characterized world wide, of which, 116 aromatic plants are recorded from Indian Himalayas. Lavender, Geranium, Lemongrass and other aroma bearing plants are the main primary ingredient for many new and existing herbal products. An attempt has been made in this communication to present an account of different aromatic wealth of Himalaya, extraction techniques and their major chemical constituents, which will be helpful to pharmaceutical industries in development of value added products in time to come.

An overwhelming proportion of plant material used for compounding herbal medicines and nutraceuticals is currently collected from the wild. With traditional health care systems gaining increasing popularity, the enormous amount of plant material extracted from the limited plant populations available in natural stands is a cause of justifiable alarm. The unscrupulous plunder of plant populations growing in the wild is pushing increasing number of important species to the endangered category and many to the verge of extinction. Also, unethical practices like adulteration and storage of medicinal plants under unacceptable conditions are rampant. All this is happening, when the consumer is becoming increasingly quality conscious and is demanding traceability of source material used for formulations and adherence to good practices for the entire chain of manufacturing process for herbal drugs and plant-based nutraceuticals. Clearly, solution to this problem lies in replacing the ‘collection from the wild ’ paradigm to ‘production by cultivation ’. An essential prerequisite for meeting this objective is a well thought out research agenda which not only tackles productivity enhancement goals for the targeted plant species but also addresses the validity of the entrenched notion among healers who use the traditional medical systems, that only material collected from the wild produces the desired results. The reason advanced in support of this argument is that phytomedicines are derived from plant populations growing in the wild under organic conditions and under their natural rhizosphere and rhizoplane. The associated soil-microbial complexes, and a variety of endophytic microorganisms, may be the initiators or modifiers of the production and expression of medicinal properties.

It also so happens that a majority of medicinal plants are shade loving, C4 types and will necessarily require their species-specific ambient environment for elicitation of the medicinally relevant moities.

ABSTRACT

Plants play an important role in human survival and contribute to sustainable development as they are the major source of different bioactive compounds such as taxol, digoxin, reserpine and vinblastin used directly as drug and medicines for human diseases. As plants are basic need and requirement for living, they are used as herbal remedy, and known to produce lead compounds for semi synthesis of higher activity or lower toxicity. Indian Himalaya regions are repository of more than 8,000 plants species and out of which 50% are endemic and unique due to thepresence of morphological and chemical structure of peculiar characters. Most of the plant used in Indian traditional system of medicines such as Ayurveda, Unani, Siddha or Amchi described for different formulations and ethnomedicinal applications had distributional range restricted to the hills and the valleys of Himalayas. Hence, in this communication, an attempt has been made to present economic plants of Himalayan origin used as ethnomedicine, wild food plants as vegetables, herbal teas, homemade soups, wild salads, chutney, underground snacks, alcoholic drinks, nutrients as burnt powder and other human usages. Besides, this unique Himalayan plant has potential to be used as wild source for the development of new drugs and medicines which can sustain future to human survival and had the ability to produce medicine against deadly diseases. It is estimated that 80% of the world population uses traditional medicine made from herbal botanicals to cure disease and illness. It is of the record that 122 compounds obtained from ethnomedicinal plants is globally used as drugs. This indicates that tribal knowledge on plants and drug discovery cannot be separated, and newdrugsfor uniquedeadly diseases can be discovered only from ethnobotanical leads. More than 5,000 species reported from Indian Himalayas are of ethnobotanical values and they are used as medicine or wild food by different tribal communities. It can be concluded that Himalayan tribals are endowed with strong culture associated with botanicals and have age-old tradition with respect to the use of wild plants as food and medicine. Plants provide a real substitute for the primary health care systems in hills and valleys, and definitely going to serve as jewel for mankind in near future to come.

ABSTRACT

In Ayurvedic system of medicine, the word Astavarga represents eight endangered Himalayan herbs, which includes four orchids Habenaria intermedia D.Don, Platanthera edgeworthii Hook.f., Crepidium acuminatum D.Don, Malaxis muscifera Lindl., three liliaceae members (Lilium polyphyllum D.Don, Polygonatum verticillatum L.,Polygonatum cirrhifolium Wall.) and one zingiber (Roscoea purpurea Sm.). These herbs are popular in various ayurvedic formulations, as for instance, Chyawanprash which is well recognized for strengthening vital force to the body, help in cell regeneration, and build immune system. These plants are repository of various bioactive ingredients such as diosgenin, β-sitosterol, catechin, p-coumaric acid and many more which shows positive biological functions such as anti-microbial, anti-oxidant, antiinflammatory and other associated health promoting activities. In traditional system, different parts of these plants are used for different purpose, and mainly rhizomes are used as herbal formulations. In terms of ecology, these plants are growing wild in Himalaya belts between the elevation ranges of 1200-4000m above mean sea level. Subtropical, temperate and alpine environment provide condusive adaptation and habitat for these species to grow and florish. Due to lack of proper documentation and certification, the identification of most of the species became difficult and illusory. Considering the medicinal importance and endangerment status, attempts have been made in the present communication to provide a comprehensive account on phytochemistry and pharmacological activities associated with astavarga species. The data presented in this communication will put door open for various research activities related to discovery of new drugs and nutraceutical products for human welfare in days to come.

ABSTRACT

Drug discovery is the process to identify the active ingredients from traditional remedies as new candidate for medications. It involves the sources, identification of screening hits, medicinal chemistry and optimization of those hits to increase the affinity, potency, reduce the potential of side effects, increase the half life and oral bioavailability followed by clinical trials. While studying and screening the plant resourses of Himalaya, Colebrookea oppositifolia Sm. is identified as an important Heptoprotective Phytopharma plant. This is an important Indian medicinal plant of the family lamiaceae endemic to the Southeast Asia. The active ingredients of this species possess antimicrobial, antifungal and antioxidant properties as evident from experimentation which may be due to the high content of flavonoids and polyphenols as a major chemical constituents. In Ayurvedic traditional system of medicine, leaves of C. oppositifolia is used for the treatment of wounds and fractures, where as roots helps in curing epilepsy. Chemical investigations on the petroleum ether extract of the leavesof C.oppositifolia, some active molecules suchasn-triacontane, acetyl alcohol, 32-hydroxydotriacontyl ferulate, β-sitosterol and 5,6,7,42 -tetramethoxy flavones have been identified which has hepatoprotective properties as evident from biological investigation. Structures of these compounds were interpreted on the basis of spectral (IR, NMR, MS) data. While studying acute toxicity of these bioactive ingredients, the species can be classified as category V as per GHS of OECD test guidelines and principles of GLP. In the present communication, an attempt has been made to provide scientific data on biology, natural products, constituents of volatile oils, salient markers, medicinal chemistry, results showing hepatoprotective activity and future direction for research to be carried out on this plant species. It also provide data on lyophilized extract or at least one bioactive fraction obtained from leaves of C. oppositifolia and treatment of liver toxicity caused by the conditions hepatoprotective against alcohol induced and tetrachloride (CCl₄) models. The presented data will be helpful in future for drug discovery.

ABSTRACT

Dysoxylum binectariferum Hook.f. (Meliaceae) is phylogenetically related to the Ayurvedic plant D. malabaricum. D. binectariferum has been identified as an enriched source of rohitukine, an alkaloid. Two synthetic flavones, flavopiridol (Alvocidib; L868275; HMR-1275; NSC 649890 of Sanofi-Aventis + NCI) and P-276-00 (Piramal) are advanced in clinical trials for the treatment of cáncer. These two molecules are considered as potent Cdk inhibitors. Cdk has been discovered as a key regulator of cell cycle. Therefore, Cdk inhibitors or modulators are of great interest to explore as novel therapeutic agents. These two molecules can be considered as rohitukine inspired drugs since their stuructural novelity originated from a compaign to develop a route of total synthesis of rohitukine. Recently a semisynthetic derivative of rohitukine; IIIM-290 has been emerged as potential preclinical cyclin-dependent kinase (Cdk) inhibitor. Moreover, phytochemical investigation of D. binectariferum yielded dysoline a new region-isomer of rohitukine. Dysoline seems to be a potent inflammatory and cytotoxic compound. Similarly, camptothecine and 9-methoxycamptothecine were isolated from Dysoxylum binectariferum for the first time by bioassay-guided fractionation. Present chapter is reviewing some original work which were carried out at CSIR-Indian Institute of Integrative Medicine, Jammu, India. The present studies focused on the phytochemistry of Dysoxylum binectariferum; dysoline, camptothecin and 9-methoxycamptothecin and medcinal chemistry and preclinical evaluation of IIIM-290 a semi-sythestic derivative of rohitukine.

ABSTRACT

Zanthoxylum armatum DC. is a plant species belonging to flowering family Rutaceae which has medicinal application in traditional Ayurvedic system of medicine. It is characterized by trifoliolate leaves with leaf-stalk winged, branches with straight prickles, and fruits are small reddish brown. In India, tribal community used this species as carminative, dyspepsia, tooth curing, treating malaria fever, stomach pain and expelling roundworms. Chemistry indicates that Z. armatum is a repository of many chemical compounds such as alkaloids, flavonoids, sterols and triterpenoids. Zanthonitrile, berberine, α-sitosterol, armatamide, limonene and linalool are major constituents. Leaves and young shoots yield essential oils which act as anti-bacterial, antifungal and anthelminthics, and has tremendous potential in development of value added products. Volatile oils are active constituentsof linalool, limonene and ligan. Z. armatum has been declared as an endangered species as per International Union for Conservation of Nature and Natural Resources(IUCN) Red List category. Conservation practices needs to be adopted to conserve this valuable medicinal plant. There is a need to study on ecology, adaptive biology, chemistry and biotechnological intervention for future propagation and conservation of this medicinal plant species.

ABSTRACT

Grewia asiatica L. locally called ’Phalsa’ (Family Tiliaceae) is a multipurpose gregarious plants species native to Asian belts, grows profusely in tropical and subtropical forests. Plant yield berries rich in calories, proteins, carbohydrates, dietary fibers, vitamins, macro-and micro-nutrients such as calcium, phosphorus and sodium. Their parts can be used as herbal medicine for treatment of various diseases. Recently, CSIR-Indian Institute of Integrative Medicine Jammu (IIIM) has developed and released a variety of this plant for rural upliftment and for development of value-added products such health drink and nutraceuticals. From its matured fruits of cultivated variety, a value added product called Shivalik Health Drink was developed by IIIM. For extension, agrotechnology of this plant has been transferred to various geographic regions of India such as Punjab, Rajasthan, Jammu & Kashmir, Uttar Pradesh, Maharashtra and Andhra Pradesh. This species prefers hardy and can be able to withstand in drought environment. For development of drugs and value added products, several therapeutic research works have been carried out which suggested that this plant species possess antioxidant, antidiabetic, antimicrobial, hepatoprotective, antifertility, antifungal, analgesic and antiviral activities. Further, there is a need to focus on biotechnological intervention with aim to develop seedless varieties and new nutraceutical products.

ABSTRACT

Panax L. is an important medicinal plant species complex, which is commonly known as ginseng. Ginseng has high commercial value for itsginsenoside contents and is collected from the wildin the subtropical-temperate forests of north-eastern India. Taxonomy, ecology and phenology of five species of Panax found in north-eastern India viz., P. assamicus R. N. Banerjee, P. bipinnatifidus Seem., P. pseudoginseng Wall., P. variabilis J. Wenand P. sokpayensis Sharma& Pandit, have beendiscussed in this paper. The distribution of these species is restricted to the states of Arunachal Pradesh, Manipur, Meghalaya, Nagaland, Sikkim and Darjeeling HillsofWest Bengal.P. pseudoginseng hasextended distribution range in Nepal. P. variabilis was reported from Nagaland, which is a new record for India. All the species of Panax prefer shady and moist habitat. The species grow in the soil texture that ranges from loamy sand to sandy. The pH ranges from 4.3-7.1. The species is perennial and pereniates through its underground rhizome. The phenophase starts with emergence of seedlings in the late March and senescence takes place in the middle of October after seed setting. Monitoring of the populations of all Panax species revealed that the population size have decreased by 40-50% over a period of 10 years due to over-harvesting from the wild by the local people for its medicinal value. Sustainable use of rhizome and its cultivation practice need to be standardized to address the conservation issues related to the plant.

ABSTRACT

Butea monosperma (Lam.) Taub, knownas ’Flameof the Forest’is used in Indian traditional system of medicine, such as Siddha Ayurveda, and Unani due to its medicinal properties. It is used in treatment of diabetes, diarrhoea, sore throat and reported to possess several biological activities such as anti-asthmatic, anti-diabetic, antifertility, anti-tumor and osteogenic. Recent studies revealed the phytochemicals responsible for osteogenic activity in the bark of B. monosperma. An ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed for identification and simultaneous quantitation of bioactive compounds from B. monosperma. Validation of the developed method was performed according to international conference on harmonization guidelines. Quantitative interpretation showed significant variations in the content of bioactive compounds in different parts of the plant and samples collected from three geographical regions. Principal component analysis was also used to study the quantitative variations of markers.

ABSTRACT

The current paper discusses the diversity, distribution and commercial importance of Indian Magnolias. Globally, these species are found in tropical, subtropical and temperate forests of southeastern Asia and tropical America. In India, there are 25 species of Magnolias and most of them are distributed in Northeast India, with highest number in Assam. Of all the species, two (Magnolia gustavii and M. pleiocarpa) are critically endangered, one (M. pealiana) endangered, two (M. manii and M. nilagirica) vulnerable, nine least concern and ten data deficient at global level. The highly threatened nature of most of the magnolias species calls for their immediate conservation and protection measures. Members of this genus are known to be rich in a wide variety of biologically active compounds including alkaloids, flavonoids, lignans, neolignans and terpenoids. Many of the species have been found to possess potent procognitive activity, anti-oxidative, anti-microbial, anti-inflammatory, anti-angiogenic, diuretic, anti-ulcer, analgesic, anti-helmintholytic, and anti-cancer activities. The species have huge economic potential and are used for a number of purposes including ornamental, medicinal, culinary, timber and joinery works.

ABSTRACT

Dermatology is the branch of medicine concerned with the diagnosis and treatment of skin disorders. Owing to the lack of proper hygiene and sanitation, incidence of skin diseases is a major health burden particularly in developing and under-developed countries. The study aimed at documenting the plants traditionally used to cure different types of dermatological disorders by the tribals and natives of district Udhampur. Ethnomedicinal data was collected by interviewing 91 infomants between the age group 26-89 years. Quantitative approaches were used to determine Use value (UV). The study revealed that a total of 64 plant species belonging to 59 genera and 43 families were used by the tribals and natives of district Udhampur for the treatment of 25 ailments of skin disorders. The most prevalent families were Asteraceae, Convolvulaceae, Rosaceae, Solanaceae and Euphorbiaceae. Majority of the taxa were growing in wild. Among the different plant parts used, leaf was the most frequently used. About 90% of medicinal preparations were applied topically. The most important medicinal species as per the use-value (UV) included Phyllanthus emblica, Aloe vera, Ficus carica, and Cannabis sativa. The present investigation may serve as a baseline data to initiate further research for the discovery of new bioactive compounds, thereby, providing important leads in the development of novel drugs for the treatment of such disorders.

ABSTRACT

Picrorhiza kurroa Royle ex Benth.is a perennial herbaceous plant species of the family Scrophulariaceae growing at high altitude regions of Himalayas, and recorded to have a wide application in Ayurvedic system of medicine known by the name of kutki. In traditional system, this plant species is used to cure disorders of liver, respiratory tract fever, dyspepsia, chronic diarrhea and scorpion sting. P. kurroa is a growing source of interest because of having number of interesting activities such as immnunomudulatory, hepatoprotective and anticancer, highly used by pharmaceutical companies for development of new molecules and herbal botanical formulations. It has been scientifically validated that this species is a repository of bioactive chemicals such as picrosides, kutkoside, and cucurbitacins isolated and characterized mainly from the roots of this plant. This communication deals with the chemistry and biological activities associated with its main chemical constituents, and can be helpful in development of new drug as medicine. This communication will provide a baseline information and useful tool for researchers who work on the area of natural products, and look for new direction in drug discovery.

ABSTRACT

Wetlands play an important role in provisioning of numerous goods and services for mankind. However, the type and the quantum of ecological services differs amongst wetlands. In the present study, we documented the various plant resources used by the riparian communities of two well-recognized wetlands, Dhir beel and Diplai beel, located in Brahmaputra Valley of Assam. The findings of study indicate that the riparian communities are dependent on wetlands for various purposes. The riparian communities uses 48 plant species as vegetables, fruits, fodder, fuel wood, medicine, roofing materials, craft making materials, bio-fertilizer and fish poisoning agent. Some of the plant species such as Enhydra fluctuans, Lasia spinosa, Colocasia esculenta, Diplazium esculentum, Ipomoea aquatica and Monocharia hastata aresold in the village market as local vegetables. The wetlands generate income for the riparian communities and save cashof many households settled plant near the wetland. Nevertheless, this study also revealed that some near the wetland resources are declining due to population pressure, encroachment near the wetlands, deforestation and over-exploitation of the wetland habitats for creation of many fishery ponds within the wetland and cultivation practices on wetland edges. The socio-economic condition, unemployment, easy accessibility and lack of alternativesources are plausibly themajor factors, whichare leading to the decline and depletion of the wetland resources. Therefore, appropriate management strategies are required for the conservation and the sustainable utilization of these plant resources.

ABSTRACT

Boswellia serrata Roxb. ex Colebr. known as ’Indian Frankincense’ is a multiferous medicinal plants known for boswellic acid and it has application in traditional ayurvedic formulation. The plant possess anti-rheumatic, anti-pyretic, anti-cancerous, anti-inflammatory, anti-hyperlipidemic, anti-coronary, analgesic and hepatoprotective properties due to the presence of several loaded bioactive compounds such as α-amyrins, β-boswellic acid, acetyl-β-boswellic acid, 11-ketoβ-boswellic acid, acetyl-11-keto-β-boswellic acid, tetracyclic triterpenoic acids and several others in minor quantities in different parts of plants. Mostly oleo-gum resin is usually used in Indian system of medicine, and acetyl-11-keto-β-boswellic acid (AKBA) is the most potent 5-lipoxygenase enzyme inhibitor responsible for inflammation, anti-cancer (G1 phase arrest) and other group of enzymes liable for allied activities. Besides, it yields essential oils and its major constituents are monoterpenoids such as pinene (2.05-64.7%), cisverbenol (1.97%), trans-pinocarveol (1.80%), borneol (1.78%), myrcene (1.71%), verbenone (1.71%), limonene (1.42%), thuja-2,4(10)-diene (1.18%) and p-cymene (1.0%). The copaene is the only sesquiterpene reported in minor quantities from essential oils of this species. Its essential show different actvities in relation to anti-cancer and antiinflammatory. From future perspectives, there is a need to carry out more research on its constituents for the development of value added products and drugs. Investigation on ecology and application of biotechnological intervention would helps in varities improvememnt, cultivation and conservation of this species.

ABSTRACT

Phosphorus is a macro nutrient, acts as a limiting factor in most ecosystems. Phosphorus immobilization is a major issue in most agricultural soils of India. Phosphate Solubilizing Bacteria (PSB) help in the solubilization of insoluble mineral phosphates by the production of organic acids. The aim of this research was to isolate, identify and characterize phosphate solubilizing bacteria from different agricultural crop soils of river banks of Yamuna, Delhi. Experiments were established in the laboratory to isolate phosphate solubilizing bacteria from various soil samples. Biochemical tests were performed to identify and characterize phosphate solubilizing bacteria. The phosphate solubilization efficiency of bacteria was measured. Organic acid production by different PSB for solubilization of insoluble phosphates were estimated. PSB from the soils of four agricultural crops such as Fennel, Spinach, Coriander and Mustard were isolated, identified and their ability to solubilize mineral phosphates by the production of organic acids were studied. Different PSB isolated were characterized as Citrobacter sp., Pseudomonas sp., Staphylococcus sp. and Bacillus sp. The PSB varied in phosphate solubilizing efficiency and production of organic acids. Pseudomonas sp. produced the maximum amount of organic acids and hence was the most efficient PSB. These PSB can be used as biofertilizers instead of chemical fertilizers and lead to sustainable agriculture.

ABSTRACT

Arunachal Pradesh in Northeastern India covers a major portion of the Eastern Himalayas and is well-known for its rich biological and cultural diversity. The indigenous communities of the state living in close vicinity of forests depend upon wild plant-resources for meeting their various needs like medicine, food, fodder, fuel, etc. The present paper deals with documentation of plant species used by Monpa and Adi community residing in vast ecological range starting from subtropical to alpine ecosystems. A total of 346 species belonging to 215 genera and 102 families used by the two communities as wild vegetables (97 species), wild fruits (39), dye and color (13), spices and condiments (51), hunting and piscicide (40), paper pulp and fiber yielding plants (5) and medicinal use were reported (165). This study highlights the rich diversity of indigenous knowledge among the local communities in utilizing the diverse plant resources around them for their sustenance. Documentation of this knowledge is of utmost importance for identification of useful plant species for value addition, promotion as well as to formulate strategies for their sustainable utilization and conservation. This would also enhance the livelihood of the indigenous communities contribute to biodiversity conservation in these fragile mountain ecosystems.

ABSTRACT

Herbal systems of medicine have been acceded by various drug regulatory authorities across the globe. In modern days, people prefer herbal medicines for the cure of various gentle to extreme and chronic disease, and another option is of allopathic system of medicine. In the present communication, the herbal formulations utilize extracted constituents of the various parts of Withania somnifera (L.) Dunal, a member of the famly Solanaceae, and commonly known as Ashwagandha, Indian ginseng, Asgand or winter cherry. This is a principal medicinal plant that has been used in various Ayurvedic formulations from more than 3000 years. Wide distribuion of this species have been reported from the various Indian states like are Gujarat, Madhya Pradesh, Maharashtra, Rajasthan, Uttar Pradesh, Punjab and mountainous regions of Himachal Pradesh and Jammu. This plant is commonly used, either alone or in combination, with other herbs for the cure of various conditions e.g stress and inflammation. The major phytochemicals of this species are alkaloids and steroidal lactones, and due to presence of various bioactive compounds, this plant species possess various multitudinous biological activities such as adaptogenic, antibiotic, aboritifacient, aphrodisiac, astringent, anti-inflammatory, diuretic, sedative, deobstruent, anti-stress, anti-oxidant, anti-carcinogenic, anti-aging, cardio protective, hypothyroid activity, immunomodulatory. Keeping these aspects into consideration, this herb has enormous potential for the management of a number of diseases. In the market, W. somnifera is available in various forms including decoctions, infusions, ointments, powder, tablet, capsule and syrup. This communication provides an account of various chemical constituents and their associated various biological functions.

ABSTRACT

Cymbopogon khasianus (Hack.) Stapf. ex Bor. variety [IIIM (J) CK-10 Himrosa] is a commercially important aromatic grass of the family Poacae and contains high valued volatile constituents which has high demand in pharmaceutical, flavour, fragrance and cosmatic industries. This plant variety is one of the rich source of two monoterpenoids, geraniol and ocimene. Geranoil is an alcohol frequently used as terpenoid fragrance material, and ocimene is a group of isomeric hydrocarbons. The present communication deals with the herb yields, volatile constituents and agrotechnology of CK-10 Himrosa. The volatile constituents vary from season to season and from geographic locations of cultivation. Data presented were collected from field trials and experiment works conducted on farmer field at Balesar area of district Jodhpur (Rajasthan). It has been observed that [IIIM (J) CK-10 Himrosa] is rich in geraniol whose percentage varies from 70-80%, cisocimene (10-11%) trans-cimene (5-6%) geranyl acetate (2-3%), and various others constituents present in minor quantities. For analysis of volatile constituents, Gas Chromatography–Flame Ionization Detector (GC-FID) and GC-Mass Spectrometry (GC–MS) methods were developed. Agronomic data indicate substantial variations in the essential oil compositions, which varies due to season of harvest and place of cultivation. This crop is hardy in nature and from future perspectives; extension of these aromatic crops in rainfed and saline areas could brought un-utilized barenlands and wastelands under cultivation. Biomass and essential oil obtained from this crop would be helpful in development of new value added products. This may serves as economic crop for marginal farmers and helpful in gaining international recognition after development of products in the form of perfumes, soaps, cosmetics and other products for human use.

ABSTRACT

Dendrophthoe falcata (L.f.) Ettingsh, a unique hemiparasitic taxa belongs to the family Loranthaceae, spreads over the entire forest in the South West Bengal. It is generally stem hemiparasitic taxa, as it is found mostly on aerial part of the host plant due to lack of root system. It depend on the host plant for mainly water and to some extend carbon. It synthesizes its own food. This plant has haustoria instead of root, they penetrate their haustoria into the vascular bundle mainly in the xylem tissue of the host plants. This species has numerous medicinal values with a long history of its use in Chinese traditional medicines. The present work deals with the anti-diabetic, phytochemicals and silver nanoparticles properties of D. falcata. The responsible enzyme i.e. Alpha-amylase breaks the large starch molecules which produces free glucose and simultaneously increases the blood sugar level and as a result hyperglycemia occurs. This study also reveals that the plant’s leaves extracts have inhibitory activities on this key alpha amylase enzyme and apart from that aqueous extract of this plant contains some secondary metabolites which is very crucial data for drug preparation and any other research purposes. The maximum amount of silver nanoparticles is present in this plant which is valuable as a silver line in near future implications for different drugs preparations

ABSTRACT

Echinacea purpurea (L.) Moench. is a medicinal and nutraceutically important herb of American origin, extended its distribution to other parts of the globe in subtropical and temperate environment. This species is known for various biological properties such as antimicrobial, immuno-stimulatory and anti-inflammatory properties due to the presence to several bioactive compounds loaded in the form of phenylpropanoids, flavonoids, terpenoids, lipids, nitrogenous compounds, carbohydrates and others such as ascorbic acid, sitosterol, linoleic acid, cyanidin glycosides and sesquiterpene esters in different quantities. Published data reveals that its major chemical constituents possess anti-anxiety, anti-depression, cytotoxicity, anti-infective and anti-mutagenicity properties. Besides, plants respond to abiotic and climatic signals that are responsible for various adaptive responses at the cellular level and vary with ecosystem in which the species grows. It may behave differently in different ecological climates, thereby, modifying their gene expressions, and this reveals that the plant growth and the adaptability are mainly affected by microclimatic factors. Experimental trials conducted to collect data on growth phenology in relation to crop-weather interaction and investigated result is presented in this research. Studies on chemical evaluation of E. purpurea under two constrasting environment, subtropical and temperate climate, with reference to cichoric acid and chlorogenic acid undertaken for this plant is also presented. Future studies in relation to the application of biotechnological interventions and chemical evaluation at different time scale would be a great contribution to the science for this species as it has the tremendous potentials which may lead to development of various nutraceuticals and value added products in future for human health and medicines.

ABSTRACT

The popular use of local plant wealth among the rural people in Hamirpur district of Himachal Pradesh reflects their greater interest in the traditional medicine and other traditional and commercial purposes. Hamirpur, one of the pioneer Himalayan district is a rich repository of medicinal flora. It is the smallest district of Himachal Pradesh due to its area, surrounded by thick forest area which is rich in diverse vegetation. Medicinal plants are used in the Ayurvedic, Unani and other traditional systems of medicine and in plant-based pharmaceutical industries. The patterns of the use of the local medicinal herbs for the treatment of various diseases and ailments have been an old practice. But the people of the district still depends upon the uses of local plants for their primary health problems treatment. This paper provides the information on the traditional uses of some of the plants such as Asparagus racemosus, Acacia catechu, Albizzia lebbek, Berberis lyceum, Bryonia laciniosa, Azadirachta indica, Adhatoda vasica, Andographis peniculata, Aegle marmelos, Celastrus peniculata, Cymbopogon flexsosus, Cuscuta reflexa and Holarrheana antidysentric of Hamirpur district for the treatment of various disorders such as anthelmintic, anti-fungal, diabetic, antibacterial, cancer, diarrhea, bronchitis, tuberculosis etc. Hence the objective of the study was to document ethnobotanical knowledge primarily of notable herbs employed by the different backward people, whether tribal or rural, in the area under study. Therefore the documentation and recording of traditional knowledge associated is necessary step for the preservation of traditional knowledge about the use of local plant wealth and conservation of plant species of study area. The data would be useful for further scientific exploration.

ABSTRACT

Himalaya represents an importantfocal point of botanical rich diversity, and homeofmanymedicinal plants known for their various therapeutic properties. Since ancient times, several plant species have been used as ethnomedicine, and only a few of these species have beenfully explored and validated scientifically. Today, cosmetic and pharmaceutical industries are the fastest growing sectors that use botanical extracts to maintain the health and support integrity of the skin and other parts of human body.Peoplearedemandingcosmeceuticaladditivesfrommarket made from plants as herbal botanicals as these causes fewer or no side effects, and therefore, ethnomedicine are gaining immence importance for cosmetic and value added products development of natural origin. Human and animal skin is the largest covering of the body as it provide barrier to the internal cells ortissues,and protectthebodyfrominfections and toxic chemicals. Keeping in mind the importance of skin, investigations and literatures were consulted to document plants protectingskininfectionsfrom external environment.Thepotential high altitude plants ofHimalaya were screened outto be used inskin care and treatmentofvarious skin pathogens.Inthe presentcommunication, total 81 plants of 72 genera belonging to 40 families are presented that have ethnobotanicalapplicationinskin care and usages incuringskindiseases. This data will provide a base-line information for cosmetic and pharmaceutical companies for developmentof various new value-added products for human and animal health care.

ABSTRACT

The present communication deals with the impact and contribution on livelihood sustainability based on forest resources and sacred places in nine fringe villages of Pangolakha Wild Life Sanctuary (PWLS), located in Sikkim Himalaya. Populations density in villages varies from 23 at Nayabusty and 274 at Padamchen. Populations were categorised under three age groups viz. minor (15-25 yrs), major (26-36 yrs) and elder (over 36 years). Approximate by 27.6% populations had minimal primary education. Agriculture is the main activity in Suvaneydara (86.7%), Agamlok (85.3%) and in Nayabustyy (82.3%). Villagers in Padamchen (12.5%) and in Nathang (12.5%) are either dependent on Government job (38.2%) or seasonal flow of tourist (25%). The educational qualification in these villages is classes 5 to 10 standards. 91.1% of people rely on forest resources. About 70% of the villagers depicts that forest cover of the region is improving. The availability of fodders (70%) and fuel woods (60%) are still in abundance. People depend on tirja (private), khashmal and goucharan areas for grazing and fuel wood collection. Forty nine economical economically by valued plant species belonging to 46 genera and 35 families were documented. Subtropical ecosystems had high number of plant species. Poaceae was the dominant family (17%). Tree life form is abundant (51%). Total 24 species are harvested for edible purpose, 24% are freely sold in the local haats (market) for their livelihood sustenance. Principal Component Analysis (PCA) of nine villages with respect to forest resources indicated the village specific use pattern of plant resources. Indigenous inherent knowledge of local plants is decreasing in Lingtam. PWLS and its adjoining villages have important places with religious importance, heritage sites managed by the people. It was observed that exploitation of plant resources, lack of maintenance and fast changing concept of traditional ethics among youths, plant resources were degraded and in regenerating stage.

ABSTRACT

Saussurea costus (Falc.) Lipsch. is a perennial rhizomatous herbaceous plant, commonly called as kuth had a wide application in drug discovery programme and indigenously known for its medicinal usage in Indian system of medicine. Wide distribution of this species recorded from Himalayas and Indo-Mynamar hotspot biodiversity regions. This species is traditionally used for treatment of several seasonal diseases happen to occurs in hilly and mountaneous regions. It has been scientifically validated that this species possess various biological activities such as anti-microbial, anti-cancer, anti-arthritic, anti-convulsant, anti-oxidant, angiogenesis effect, hepatoprotective, gastro-protective, anti-obesity, and anti-inflammatory due to the presence of various bioactive principles such as costunolide, germacrene, lappadilactone, isodihydrocostunolide, cynaropicrin, linoleic acid, cyclocostunolide, alantolactone, isoalantolactone, sesquiterpene-saussureamines and several others in minor quantities. This species naturally grows in wild and mostly collected from the wild for its herbal usages, and this wild collection activities put S. costus under threatened and critically endangered categories, hence there is need for the development of agrotechnology for cultivation in large scale. This species has the potential components for developing new molecules in future for drug development. The present communication provides traditional usages, phytochemistry, therapeutic potentials, and future perspectives of S. costus. This baseline information provided in this article will be helpful in future for research to be carried forward in drug discovery programme using this plant species.

ABSTRACT

Systematic studies through stored specimens in herbaria and museum of defined geographical areas provide a set of baseline data for understanding the vegetation composition of a defined ecosyetm. A stored plant collection in herbaria offers a rich portrait of biodiversity, making innumerable contribution to science and human society. Digitization coupled with hosting of such information on world wide web (www) system has been accepted as an important tool for the dissemination of the botanical specimens in the form of databasing, imaging and geo-referencing of voucher specimens. In the present communication, we describe the development of the Janaki Ammal Herbarium (JAH) database that contains a comprehensive and searchable data of voucher snaps, historical background and statistical analysis of 23,225 voucher specimens. The information on JAH database is easy to extract data on botanical names, author citation, variety (if any), family, collection number, specific location, collection date, habitat, collector’s name and remarks on morphology. The goal of the present investigation is to digitize (database, image, georeference) all specimensin JAH, enabling themto be made available through a single portal. At concluding, all vouchers of JAH made publicly accessible via world wide web user interfaces, and also linked to PubMed, NCBI Taxonomy, where the site provide information related to Entrez records, comments and references of the particular species, while Wiki species site is linked to the free species directory. It also provide link of online accessible herbarium-based databases of the world.

ABSTRACT

The genus Aconitum is in the limelight of the current scenario of the pharmacopeia and has became the centre of attention in the field of herbal medicines, as is manifested with various biological and pharmacological cores which make the genus most imperative. For vast decades some species of the genus Aconitum and its derivatives were considered as vizulently fatal but were liquidated as intact drug elites through traditional as well as modern system of medicine. The retrospection of the validated information in account of the genus Aconitum represent a pioneering approach to corroborate the scientific studies on almost everyaspect of the herbal genusAconitum, as a number of species belonging to this genus has been enlisted in the Red Data Book of IUCN which have been renowned for their medicinal benefits in various systems of medicine. The tubers some species of Aconitum contain lethal diterpene alkaloids such as aconitine, mesaconitine, and hypaconitine, which easily can be directed into screened harmless alkaloids such as benzoylaconine, aconine, and pyroaconine by heating or alkaline treatment. Currently, the processed tubers are widely and safely practiced for the remedyof pain, neuronal disorders, inflammation, and rheumatism and have a huge therapeutic index for curing ailments like hysteria, throat infection, dyspepsia, abdominal pain, diabetes and also as a cardiac depressant in massive arterial tension of cardiac origin. An important component extracted from some species of Aconitum, Lycaconitine has been found to be effective against multi-drug resistant cancers. The di-terpenoid alkaloids of aconite source and their increasing demand for the active compounds used in different systems of medicine has led to overharvesting of the tubers, resulting in rapid depletion of the natural stocks of this valuable plant. As a consequence, the genus is now on the list of rare and threatened species. Since the commercial demand of this important medicinal plant is very high and hence needs to be conserved as the species density of this genus has been threatned due to many factors. As such diverse efficient and standardized protocols for its regeneration like micropropagation, somatic embryogenesis and some other conventional efforts like storage of seeds as a means of ex-situ conservation must be attributed on the aspects of conservationof genusAconitum. The exploitation of population genetic diversity will provide useful information for the biological conservation of these esteemed species of Aconitum in the regions . Since a complete revision of the genus Aconitum in the region has not yet been carried out and the high level of endemism and a lack of relevant information of the genus Aconitum have made its various scientific studies a very interesting object. The scientific and systematic information about the genus Aconitum generated demands validation as only a little attention has been done on its various aspects from cultivation, conservation, phytochemistry and pharmacological evaluation to the molecular characterization. Consequently species specific measures are needed and a lot of important attributes has to be assigned to the species of this genus which will ultimately help to ensure simultaneously both conservation as well as sustainability in raw material production of the genus.

1. Introduction

Microbial diversity is becoming increasingly essential with the requirement to preserve the integrity, function and long-term sustainability of natural and managed ecosystems. However, our knowledge of the diversity of microbes remains obscure due to lack of appropriate approaches to evaluate the contribution of different components of the soil microbes to ecosystem. This lack of approaches together with the complex and heterogeneous matrix that soil microbes are embedded in, poses great challenges to understand the dynamics of microbial communities in soils (Nautiyal et al. 2010; Chauhan et al. 2013).

In classical terms, diversity is described as the total number of species present, i.e. species richness or species abundance, and the distribution of individuals among those species i.e. species evenness or species equitability. However, the incorporation of these two components into diversity indices has led to much controversy among ecologists (Atlas 1984). The Shannon-Weaver index (Shannon and Weaver 1949) is probably the most widely used index for measuring diversity of a microbial community. But this index, as many others that have been proposed, has its own limitations. A major difficulty is that both richness and evenness play a role in determining the value of the index. Therefore, any diversity index is a single value and thus cannot indicate the total make-up of a community. It is recommended that evenness, richness and diversity values should be calculated in order to obtain an objective assessment of community structure (Atlas 1984). From molecular point of view, diversity often refers to the number of different sequence type present in a habitat e.g. a soil (Ogram 2000). Measuring this type of diversity does not give information about the frequency of individual species, but it can give valuable information about changes in the community structure and species richness in response to changes in the physico-chemical properties of the soil and soil management practices (Nautiyal et al. 2010).

1. Introduction

Viruses are a unique group of infectious agents. They are distinct in their simple, acellular organization; however, they are the connecting link between live and dead. Viruses are also the perfect example of obligate intracellular parasitism. Despite their simplicity, plant viruses are extremely important and drawn attention of researches because they can be used as model to study the disease establishment and progression mechanism, reproduction inside the host and trilateral host-virus-vector relationships. Their small genome also gave liberty to extensively understand the genome evolution caused by the genetic exchange or recombination. As a consequence, this will help in the development of facile methods for management and control of new emerging viruses and their strains.

This chapter is written for the beginners and students of virology subject to provide the fundamental knowledge about the origin, history of plant virology, the general features of plant viruses, their structure, shape and size, mode of spread and development of their management strategies. Besides this, the new development of non-conventional methods for developing virus-resistance in plants has also been explained for enhancing the quality and production of commercial crops in the country.

1. Introduction

Fungi are ubiquitously present in air, water, soil and organisms, or on organism surfaces. They are a group of eukaryotic system that are of great applied and scientific interest to common man, microbiologists, agriculturists and industrialists. They are a diverse group of organisms with respect to taxonomy, size, morphological appearance, mode of nutrition and reproduction. Fungi are eukaryotic spore bearing protists that lack chlorophyll. Fungi comprise the molds and yeasts. Yeasts are usually unicellular measuring only several microns in diameter, whereas molds are filamentous and multicellular which may grow up to several meters in diameter like the extremely large polypores. Fungi generally reproduce both sexually and asexually (Gow and Gadd 1995).

Although it is not easy to define fungi, biologists currently use the term “Fungus” to include nucleated, spore bearing, achlorophyllous organisms with absorptive nutrition which generally reproduce sexually and asexually, and whose filamentous branched somatic structures are typically surrounded by cell walls containing cellulose of chitin or both. A feature which all fungi have in common is their mode of nutrition. Like animals, fungi are heterotrophic organisms, which must consume preformed organic matter. They may live as saprophyte or parasites. They are of great economic importance to man and play an important role in the disintegration of organic matter and affect us directly by destroying food, fabric, leather and other commercial goods. They are responsible for a large number of diseases of plants, animals and man.

1. Introduction

Lichens are the most fascinating and widely distributed organisms on earth. The lichens are unique in having two microorganism in a single plant- a phycobiont (alga) and a mycobiont (fungus) forming a thallus that does not resemble either symbionts in the free living (non- lichenized) state. By virtue of the peculiar structure and physiology, lichens have high tolerance of drought and cold and are able to grow in the diverse geographical regions from icy expanses to tropical and subtropical parts and from drier, hot deserts to moist humid climate. Lichens grow on any substratum that provides a convenient foot hold to them. This may be soil (terricolous), humus (humicolous), stones, rocks, brick (saxicolous), lime plaster (calcicolous), leaves (folicolous), tree trunk (corticolous), decaying wood (lignicolous) and other man made substratum like iron pipes, asbestos sheet and glass panes. Sometimes lichens also grow on some insects and animals. Lichens which are bigger in size and shape can be easily recognized as leaf like (foliose) and thread like (fruticose) (commonly called macrolichens), while those which forming a crust over the substratum and quite smaller in size are categorized under microlichens.

Apart from altitudinal variation, the vegetation and forest types of higher plants also play an important role in determining the type of lichen flora of the region. Based on the forest types six lichen vegetation zones are known in India such as: Moist tropical evergreen forest, Cold deserts in the Himalayas, South Peninsular region, Mid Eastern Indian & Peninsular Plateau, Dry and arid regions and Indo-gangetic plains of central India, Coastal regions of India and Andaman & Nicobar Islands. The cold deserts in the Himalayas exhibit some unique group of lichens having restricted distribution only in such habitats.

1. Introduction

The word ‘lichen’ has a Greek origin, which was referred to the superficial growth of fungus like organism on the bark of olive trees. Theophrastus, the Father of Botany coined the term ‘lichen’ during 300 BC and introduced this group of plants to the scientific world.

Lichen is a combination of two organisms, an alga and a fungus, living together in symbiotic association. Sometimes instead of an alga a cyanobacterium (blue-green alga) may be present in the lichen thallus. The algal component in the lichen is generally called as ‘photobiont’ as it contains photosynthetic pigment. The fungal part is called as ‘mycobiont’ (myco = fungus). The photobiont and the mycobiont loose their original identity in symbiotic association and the resulting organism is the lichen which behaves as a single organism, both morphologically and physiologically. Hence, the lichen is called as a composite organism. The lichen thallus is made 90% of mycobiont which provides shape, structure and colour to the lichen. In a lichen thallus whatever visible from outside is fungal part, which holds algae inside its body (Fig. 1) and therefore, the lichens are placed within the Kingdom - Fungi. The fungi present in lichens are called as ‘lichenized fungi’. Among the 20,000 lichen species present in the world, 98.9% of them belongs to Ascomycetes group while Bacidiomycetes and Deuteriomycetes groups are represented by only 0.1% and 1%, respectively.

1. Introduction

India, as a party to the Convention on Biological Diversity (CBD), has obliged to document a whole range of organism diversity within her territorial boundaries, make all attempts to conserve these bio-resources and monitor the efficacy of the conservation measures adopted. With its diverse ecological conditions and unique geographical location, Indian landmass supports rich diversity of algal flora. However, this algal wealth needs to be systematically surveyed and documented from all possible habitats. Ecological patterns emerged from recent studies within India are assessed with the identification of knowledge gaps and conservation implications in algal systematics.

During last one decade, great advances have been made in the field of Algology/Phycology throughout the world. Over the years algae has attained a greater reputation and popularity because of its economic importance, particularly as food, for commercial products, water contamination, toxin production, sewage oxidation, municipal water purification in fisheries and above all for the study of fundamental scientific information. But before the algae put in to such important use, it is imperative to have a baseline data about the material in question. Without knowing the status of algal resources of any area one cannot have projections for their utilization or gain knowledge of their ecological role and functions. The taxonomy of algae is, therefore, a prerequisite for all applied fields of algae apart from academic interest. Recent global surveys have revealed that there is lack of knowledge about fresh water algal diversity of tropical countries including India.

1. Introduction

The objective of algal taxonomic collections is to represent the natural population, flora and diversity in size and form. Therefore, it is important to collect the representative samples seasonally from various habitats. Collection of information about the habitats and other details are also very important. For algal sampling the first thing to consider is how to collect the specimens. The freshwater algae show an ability to tolerate a wide range of environmental condition. Under natural conditions, they usually grow in mixed communities which may include many species and genera. Field study provides information about the site, environment, season and size and shape of the living algal thallus in its natural surroundings. Many algae can be tentatively identified by their appearance, shape and colour of thallus in a particular habitat. Algal population can be sampled in a number of different ways, depending on the nature of the habitat and the location of the algae within the water body. The specimens must be prepared carefully so that important morphological characters are displayed as fully and completely for further researches.

1. Introduction

Bryophytes are atracheate, archegoniate non-flowering group of highly diversified plants. Among plants they form the second largest group comprising of 15000 – 25,000 species (Gradstein et al. 2001, Crum 2001). They occur in every continent on globe, in every location habitable by photosynthetic plants and grow luxuriantly between altitude of 1000-3500m. In India, nearly 850 species of liverworts, 41 species of hornworts and 2000 species of mosses, such as are known so far (Singh 1997, Vohra and Aziz 1997). These plants exhibit a variable range of size as some of them are only 0.5-2 mm in size, while on the other end mosses Polytrichum commune and Dawsonia superba can attain a height of 50 and 70 cm, respectively. These plants play a vital role in soil conservation and formation of fertile substrata for other plants in forest ecosystem. On account of unique moisture retaining capacity, Sphagnum and other pleurocarpous mosses are being used as mulching substances, moss grass, moss sticks and bags in horticulture industry. These plants are very sensitive to environmental pollution due to simple structure and devoid of cuticle, and accumulate heavy metals too, hence serve as good bio-monitor of pollution. Some novel compounds viz., marchantin, riccardin, lunularic acid, terpenes and other phenolic compounds have been detected in several thalloid as well as leafy liverworts.

Bryophytes exhibit heteromorphic alternation of generations in which the gametophytic phase (haploid phase) is a prominent and nutritionally self- sufficient independent phase in the life cycle while the sporophyte (diploid phase) is attached and dependent on the gametophyte. The main plant body (Gametophyte) is either thalloid or leafy (differentiated into axis and leaves). They are attached to substratum by simple hair like structures called as rhizoids. The rhizoids are usually unicellular, simple (smooth walled) in hornworts; simple, tuberculate or sinuate in liverworts or multicellular, oblique septate in mosses. Sex organs, antheridia and archegonia produce antherozoids and eggs respectively. After fertilization the zygote is formed which develops into the sporophyte. The sporophyte consists of foot, seta and capsule. The foot is embedded in the gametophytic tissue and draws nourishment for the development of sporophyte. Seta is generally stalk like may be of variable length which holds the capsule. The capsule is the main fertile portion of the sporophytic generation. The spore mother cells, after meiotic division, form spore tetrads having haploid spores. The elater mother cells form elaters, which are sterile and help in the dispersal of spores. Bryophytes are homosporous. Spores germinate to form young gametophyte in liverworts and hornworts, while in mosses, spores first give rise to protonema then buds develop on the protonema to form new gametophytes.

1. Introduction

Pteridophytes consist of ferns and their allies and are one of the oldest groups of plants on the earth, and they are commonly known as vascular cryptogams. Pteridophytes have a long history of evolution and were the dominant group of plants during the carboniferous period. The ferns, which constitute major element of the pteridophytes, preferentially grow in shade, moist habitats with moderate temperature but also occur throughout from high altitude in arctic-alpine to rain forests of tropic regions of the world. The most favoured habitat of these plants, however, is tropical mesic environment, where 65% of the living species of fernsthrive vigorously and grow luxuriantly (Page, 1979). Ferns are distinct in having rhizomes, stipes, fronds and are homosporous, whereas the fern allies exhibit scale like leaves and are heterosporous (mega and micro). In ferns, the spore matures into gametophyte that bears both male and female gametangia in a definite pattern. In fern allies, both the mega and microspore mature into female and male gametophyte that bears female and male gametangia, respectively. Pteridophytes are unique in having two different phases-the gametophyte and sporophyte. There has been a wide range of variation in spatial distribution, habitat, growth pattern and morphology of pteridophytes. Fern and fern allies, are comprised of six distantly related classes viz. Lycopodiopsida, Selaginellopsida, Isoetopsida, Equisetopsida, Psiloptopsida and Polypodiopsida (Copeland 1947; Pichi Sermoli 1987).

There are about 12000 species of the pteridophytes belonging to 300 genera in the world, whereas India is represented by about 1200 species (Chandra, 2000). Beddome (1892) during the first quarter of the twentieth century provided a comprehensive account on ferns of British India, and subsequently, Clarke (1880) and Hope (1899, 1900, 1902, 1903, 1904) made significant contributions in taxonomic studies of pteridophytes mainly the ferns of north western India. Several workers (Nayar and Kaur 1974; Dixit 1984; Chandra and Kaur 1987, 1994; Manickam et al. 1992; Khullar 1994, 2000; Chandra 2000; Singh 2003; Singh and Panigrahi 2005a, 2005b) have significantly contributed towards the taxonomy of Indian pteridophytes.

1. Introduction

The gymnosperms constitute a distinct subdivision of spermatophytes, the seed bearing plants. The term ‘gymnosperm’ is derived from Greek words, gymnos, naked and sperma, seed, which indicates that these are naked-seeded plants, i.e., those in which the seeds are not enclosed within the fruits but are borne freely exposed on an open carpel. In contrast in the angiosperms the seeds are enclosed within a closed carpel (ovary). The first known use of the term gymnosperm was given by Theophrastus in 300 BC in his book “Enquiry into Plants” to describe “plants with unprotected seeds”. Goebel (1905) rightly described gymnosperms as “Phanerogams without ovary”. The Scottish botanist, Robert Brown in 1825 first distinguished gymnosperms from angiosperms. At one time they were considered to be single class of seed plants, called Gymnospermae, but taxonomists are now tend to recognise four distinct divisions of extant gymnospermous plants - coniferophyta (Pinophyta), Cycadophyta, Ginkgophyta and Gnetophyta. Geological history reveals that all divisions of gymnosperms have been distinct groups since their origin.

Among the gymnosperms are plants with stems that may barely project above the ground and others develop into the largest trees on the earth. The extant gymnosperms are a small group of plants comprising ca. 1026 species belonging to 83 genera and 12 families (Christenhusz et al. 2011). They are distributed throughout the world, with extensive latitudinal and longitudinal ranges. Economically, gymnosperms are of outstanding value, fulfilling the greater proportion of our timber as well as resin requirements. They are also the dominant forest makers of the world and in addition they play a leading role in the preservation of the environment (Sahni 1990). By far the largest group of living gymnosperms is the conifers (pines, cypresses and relatives), followed by cycads and Gnetales (Gnetum, Ephedra and Welwitschia).

1. Introduction

Among three major functions identification, classification and nomenclature of taxonomy (Fig. 1), identification is a core activity and one of the major objectives of taxonomy, which determines whether other two elements are the same or different. Classification and nomenclature are two separate methods in taxonomy, however, both are totally dependent on the correct identification of the objects. The identification of an unidentified plant with a correctly identified specimen also involves classification. So, both identification and classification, involve comparison and judgment of similarities and differences. Therefore, the identification is a primary function in classification involving nomenclature which performs an essential role as a means of communication. According to Blackwelder (1967) “identification enables us to retrieve the appropriate facts from the system (classification) to be associated with some specimen at hand” and is “better described as the recovery side of taxonomy.” The identification of plants is very important for proper assessment and characterization of biodiversity on which the whole life of human beings is dependent starting from birth to death. The use of biodiversity for various purposes like food, nutrition, pharmacognosy etc. requires accurate and reliable identification and naming of plants (Nesbitt et al. 2010).

1. Introduction

Herbarium is simply a dried and pressed collection of plants arranged in an accepted sequence of classification (Lawrence 1951, Shetler 1969). It can be a very useful teaching aid or an absorbing hobby (Cronquist 1966). According to Fosberg and Sachet (1965) “A modern herbarium is a great filing system for information about plants, both primarily in the form of actual specimens of plants and secondarily in the form of published information, pictures and recorded notes”. It is also a research, training and service institution that serves as a reference centre, documentation facility and data store house (Radford 1986). It is now well recognized that a herbarium is a vast reservoir of facts about plants. The word ‘Herbarium’ was originally applied, not to a collection of plants but to a book dealing with medicinal herbs. Tournefort in about 1700 used the term as an equivalent to ‘Hortus Siccus’ which was later on adopted by Linnaeus (Stearn 1957). The present concept and development of herbarium is due to efforts of botanists for more than four centuries. The concept of herbarium has been changing continuously, which in turn, has affected the gradual development of herbarium and herbarium practices.

For practical reasons, the classification of the world’s flora is primarily based on herbarium material and the literature associated with it. Despite its limitations, a herbarium has certain advantages over living collections. It is usually only in the herbarium that we can compare all the related species of a genus in the same place, in the same state and at the same time (Davis 1961).

The herbarium material, therefore, formed the foundation of all botanical studies. We should, therefore, realise that herbaria are the custodians of enormous data pertaining to plants. They are usually seen as old-fashioned and expensive “stamp collection” without realising their paramount role in biological sciences. However, the world-wide rise of interest in the field of conservation, biodiversity and bioprospection offer a new chance for herbaria. Furthermore, computers offer a golden opportunity to raise the status of herbarium taxonomy by making routine curatorial procedures more efficient.

Although Jain and Rao (1976) elaborately discussed the methods of field collections, identification and preservation in the herbarium, the salient features of herbarium in up dated form with various procedures followed in Indian context are discussed in this chapter.