eChapters

Damage to arable land tends to attract most attention but degradation of non-arable land can also have serious effects, whether economic, or social, or on the food supply. In dry seasons, loss of grazing capacity is a common and serious problem, forest land may suffer through over-exploitation, roads can cause much damage particularly on steep land. In this chapter we will look at the more important erosion problems on non-arable land.

13.1 Erosion on grazing land

On well-managed high-yielding pasture it is unlikely that there will be any erosion, for the ground will have a good uniform cover. But land which is not arable because it is too poor, too infertile, too stony, or too arid, and is only described as grazing land because it is unfit for more intensive use, is a very different matter. Again, erosion control can be directly and precisely equated to improved management - anything leading to a better water balance and a better ground cover will lead to reduced erosion.

11.1 The farmer’s point of view

In the last chapter possible conservation practices were reviewed in groups according to their purpose in terms of influencing runoff or soil loss. Now, looking at ways of limiting soil degradation by improved land husbandry, the primary consideration is to look at the alternatives from the farmer’s point of view. In fact we need to be very wary of manual-type recommendations. A better approach is to offer a menu of alternatives, and the role of the professional is to help the farmer choose what is appropriate for his or her circumstances. A recent review of technologies acceptable to resource-poor farmers points out that combining practices into a farming system must take account not only of the physical factors such as soil and climate, but equally the available resource inputs, especially cash and labour, and the farmer’s objectives (STOCKING 1993).

The objectives of a commercial farmer are usually to maximize yield and income, but the subsistence farmer is likely to be more interested in improving food security by reducing the risk of failure, or improving the return on inputs of seed, fertilizer and labour, or improving the quality of life by reducing drudgery, particularly relevant in the case of women farmers.

We need a new approach to the farmer when recommending changes to farming practice. Instead of recommending practices because they will reduce erosion, we should show how they can lead to increased production. For example, practices which lead to better soil structure, more organic matter, or more moisture-holding capacity should be put forward because they improve yield, not because they reduce the soil erodibility. Increasing infiltration from better ground cover or the use of contour farming or ridging can result in better crops as well as reducing soil loss. Improved crop management could include more use of fertilizer and manures or crop rotations. These practices will make more sense to the farmer if they are recommended as leading to better production, which he can instinctively appreciate as desirable, rather than because they will reduce soil erosion which is likely to be a less important objective.

Introduction

Aquaculture, also known as aqua-farming, embodies the procedure of the farming of fish, crustaceans, molluscs, aquatic plants, algae, and other organisms. Aquaculture involves cultivation of freshwater and saltwater fish-populations under controlled conditions (Wikipedia, 2019). Aquaculture industry has undergone a lot of change in the last two decades. The application of the sophisticated technologies in the aquaculture industry has greatly altered the face of the industry in special relation to the Western and developed countries. New tools related to the measurement of the different environmental parameters needed to be controlled in different layers of the production process like Hatchery, Nursery and Grow-out have come up. Lots of data generated from the above stages relate to include the parameters, like, cost of production, health condition of produced fish, etc., which ultimately leads to the generation of the appropriate pricing of produce. With the advent of globalization, all aquaculture product companies need to look for exporting their respective products along with meeting the full satisfaction related to the demand from the domestic markets with an ultimate objective to increasing the profit. Export of the fisheries products in the western countries needs proper certification on the quality of the products duly by the appropriate authorities. The certification authorities need to check the detailed production process related to the fisheries- products in order to be sure about the quality imbibed in the same. The development of integrated software maintaining the details of the production process makes the task easy for both the production company and the certification authority, thus satisfying the needs of the end-customers in the western markets. Useful ERP software needs to maintain all relevant information on the production process commencing from the very early stage of the production till the act of selling the products in the market.

5.1 INTRODUCTION

A GIS database without single error is reliable for decision making. There are various possible sources of errors in GIS. If an error is not removed during scanning, the final output will also have that error. Besides whatever the work has been carried on wrongly scanned map it will add to errors because the source map itself's erroneous. This is like garbage in garbage out proverb. If wrong data is taken for analysis it will only result in wrong output. Hence it is important to remove error at every stage of GIS work. It is important to locate the sources of errors so that due care may be taken while handling a project.

5.2 ORIGINAL SOURCES OF ERRORS

All the equipments which give attributed data and spatial data will give errors. Before going to field or before collecting data all the related instruments should be calibrated properly. Also the instrument should not cross the permissible error. Besides these while using data from diversified sources errors pertaining to those instruments are mixed to give a different result. When very good accuracy is desired data should be collected from high end equipments. Even if one adds the data from a comparbly less accurate instrument to the data of many accurate instrument is then the result obtained finally will not reliable enough. Global Positioning System (GPS) is available with mm accuracy to meters accuracy. Also surveying with suitable map projection and datum is important; otherwise the result will be wrong. Survey instruments posses some inaccuracy and when a person is using it he should have the accuracy over the instrumental like rain gauge, water level recorders which give attributed data which should be checked for instrumental inaccuracies and method of data collection before using it. Likewise satellite and aerial sensors miss some data and some error are inherent. Such errors should be removed before moving ahead. Errors are resulted due to map reduction, enlargement, data editing and computation method adopted. At the same mapping instruments also possess inaccuracies. When after certain interval a person is going for survey, the registered object may have been changed. Many new features might have come up. Hence the established control points are changed and if the wrongly placed control points are taken for survey then the result itself will be wrong. If proper resolution is not available in the instruments, then the instruments may not capture the minute details which are required.

Introduction

The GIS database is a model of real world. It goes without saying that there is an inherent discrepancy between GIS database and the real world it represents. This is so because all models are fraught with cognitive issues and remain as approximations. To realize the objectives of a GIS, geographical data collected, entered and processed are to be sufficiently error free and reliable.

Errors can be statistical errors like mistakes, wrong inputs or faulty field surveys or system errors like excessive tolerances or high RMS error. The spatial database has further problems in the sense that the real world phenomena are not always intrinsically discrete. For instance where is the bank of a shallow river? Where is the boundary between two soil types? These are but a few questions revealing the fuzzy nature of geographic data. Many soil scientists, geographers have noticed that carefully drawn boundaries or iso-lines on maps in general give misrepresentations of change that are often gradual, vague or fuzzy. Traditional cartography as a medium of spatial data handling had a solution to such problems. But now when digital data are handled, they need to be stored with certain precision. Moreover, the spatial variation of natural phenomena is not just a local noise function or inaccuracy that can be removed by collecting more data or by increasing the precision measurement but is often a fundamental aspect of nature that occurs at all scales. (Goodchild, 1980; Mark and Aronson, 1984). This means residual unmapped variation is a remarkable problem in geographical information processing. It is therefore a prerequisite to fully understand how errors arise or propagated in GIS and what might be the effects of errors on output.

Introduction 
The mandate of the University under section 30 of the Act of 1963 is for establishment of Extension services for the purposes of under taking extension programs, dissemination of information for solving of problems relating to agriculture and domestic fields, developing the interests of youth in agriculture etc., The first extension service of APAU popularly known as District Extension Education Programmes were introduced in 1967 in Hyderabad district and were subsequently extended to Chittoor(1968)and Guntur (1970) districts. National Demonstrations Scheme (NDS) was established during 1970-71 in the University in four locations. In commemoration of the Golden Jubilee Year of ICAR, a Lab to Land Programme was launched by the APAU in June,1979. In order to improve the productivity of pulses and oilseeds in different regions of the state, two extension education centres were sanctioned by the ICAR at Ananthapur and Rastakuntubai which started functioning from December,1980 and December,1981 respectively. Reorganization of the University Extension work followed the reorganization of research activities of the APAU and implementation of Training and Visit System by the Department of Agriculture to streamline the setup of the field extension program of the University during 1982. To optimize the utilization of available extension resources, the resourcess of District Extension Education programs, extension services around research stations and NATP were integrated and reorganized to form six Extension Education Units. The extension activities of these units were entrusted with the respective Associate Directors of RARS for integrating research and action activities. The Associate directors of Research in turn were made responsible to the Director of Extension. The Extension Education Units conducted different extension activities like organization of field trials for testing new technologies for their feasibility including minikit trials of pre-released varieties to get feedback to the scientists, introduction of new cropping patterns, demonstration of latest technologies through early demonstrations including educational activities like organization of Kisan Melas training programs for the developmental

Etiology

Erysipelothrix rhusiopathiae.

Disease Occurrence

The disease is worldwide in distribution.

Host Range

Outbreaks of significant economic loss are in turkeys. Occasional outbreaks have been reported in pheasants, ducks, geese, guinea fowl, chukars, grebes and emus. Erysipelas also occurs in swine, sheep, sea mammals, fish and many wild animals. In humans (fish handlers, butchers, kitchen workers, turkeygrowers and veterinarians) it causes a localised inflammation called “erysipeloid”.

Natural Habitat
The bactrerium is widespread in nature and has been recovered from a wide variety of wild and domestic animals including mammals, fish (both fresh and salt water), birds, reptiles and amphibians. It is present in the soil and can survive for 20 days or longer in alkaline soil. The major source of infection for swine and turkeys is carrier animals of the same species. It is reported that 30-50% of pigs carry the bacterium in their tonsils, other lymphoid tissues. It is present in slurry of piglets and can be recovered from the faeces of carrier pigs.
Morpholgy
Erysipelothrix rhusiopathiae (previously named Erysipelothrix insidiosa) from S (Smooth) -form colonies and usually from acute syndromes is a grampositive rod, the R (rough) form colonies usually from chronic disease is a gram-positive filament. The organism is non-motile, non-spore forming, nonacid fast, capsulated occur either in singly, in groups or in chains.

Cultural Characters
It is a facultative anarobe, but growth is enhanced by 10% CO2. It is able to grow in a temperature range of 50C to 420C, within a pH range 6.7 to 9.2 and 8% NaCl2. Growth occurs on nutrient agar but is improved by the addition of serum or blood. It will not grow on Mac Conkey agar. Media contain either sodium azide (0.1%) or crystal violet (0.001%) may be used as selective media. On blood agar, non-haemolytic pinpoint colonies (0.5 mm) appear at 24hrs incubation. Colonial variation becomes obvious at 48hrs incubation when azone of greenish haemolysis often develops under and just around the colonies.
 

Escherichia coli is an important model organism for studying microbial metabolism, metabolic regulation, and metabolic engineering. This chapter introduces the use of metabolomics to investigate the metabolic architecture and physiological responses of E. coli. It examines metabolite profiling, central carbon metabolism, metabolic pathways, environmental responses, and metabolic flux analysis. The chapter explains how metabolomic data can reveal changes in cellular metabolism and support the identification of pathway bottlenecks and regulatory mechanisms. Applications in microbial biotechnology, recombinant production, metabolic engineering, and optimization of fermentation processes are considered. The chapter demonstrates how E. coli serves as a useful experimental system for connecting metabolite changes with cellular function.

Introduction
Escherichia coli (commonly abbreviated as E. coli) is a Gram-negative, facultative anaerobic, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms).
Escherichia coli are common inhabitants of the terminal small intestine and large intestine of mammals. They are often the most abundant facultative anaerobes in this environment. They can occasionally be isolated in association with the intestinal tract of non-mammalian animals and insects. The presence of E.coli in the environment is usually considered to reflect faecal contamination and not the ability to replicate freely outside the intestine.
Morphology
E.coli is Gram-negative, facultative anaerobic and non-sporulating. Cells are typically rod-shaped, and are about 2.0 micrometers (μm) long and 0.25- 1.0 μm in diameter, with a cell volume of 0.6–0.7 μm. It can live on a wide variety of substrates. Strains that possess flagella are motile. The flagella have a peritrichous arrangement. It is motile by peritrichous flagellae, though some strains are non-motile.Spores are not formed. Capsules and fimbriae are found in some strains.
Cultural Characteristics
Escherichia coli or E.coli cells may grow on a solid or in a liquid growth medium under a laboratory condition. Solid and liquid media may have exactly the same composition except that the solid medium contains an extra 1.5% agar. Different E.coli clones may have different properties. Colonies growing on solid media represent different clones. It is an aerobe and a facultative anaerobe. Optimal growth of E. coli occurs at 37°C (98.6°F) but some laboratory strains can multiply at temperatures of up to 49°C (120°F).

Introduction

A total of 109 elements have been discovered and incorporated into the periodic table. Many of these elements are found in the earth’s mantle, crust, and soil, though their abundance varies. The chemical makeup of plants is, to some degree, influenced by the composition of the soil. However, since plants are active organisms, they exhibit selectivity in absorbing elements from the soil. Plants accumulate elements at different rates, absorbing some in large quantities, others in smaller amounts, and excluding most altogether. Before proceeding, it is important to familiarise with several key terms frequently used in this chapter.

Nutrient: A nutrient element is essential for an organism to complete its life cycle, and a deficiency in this element leads to specific deficiency symptoms. The negative effects can only be corrected by providing that particular element. Nutrient elements essential for plants may not be necessary for other organisms, and vice versa. Depending on availability, the nutrient levels in a plant can be deficient, sufficient, or toxic. A nutrient is considered deficient when its concentration is so low that it significantly limits growth and causes recognisable deficiency symptoms. If nutrient levels cause reduced growth without visible deficiency symptoms, they are termed insufficient. The nutrient content range in plants that supports optimal yield of crops is considered sufficient. When the concentration of a nutrient becomes high enough to significantly reduce growth, it is considered toxic.

German chemist Justus Freiherr von Liebig (1803–1873), Professor at the University of Geissen and the French chemist Jean-Baptiste Joseph Dieudonne’ Boussingault (1801–1887), Professor at the University of Lyon and later Chair, Conservatoires des Artes et Matier in Paris are to be credited for analyzing the plants and coming with the idea that plants absorbed nitrogen and other mineral nutrients from the soil for their growth. D.I. Arnon and P.R. Stout, Plant Physiologists and Plant nutritionists at the University of California, Berkeley, USA, grew plants by hydroponics using pure chemical compounds and established following 3 criteria for the essentiality of nutrients for plant growth

Entrepreneurship theory has been evolving over the last 20 years and is ever growing. It is defined as a verifiable and logically coherent formulation of relationships, or underlying principles that either explain entrepreneurship, predict entrepreneurial activity or provide normative guidance (prescribing the right action in particular circumstance). Entreprenuership is interdisciplinary and contains various approaches that would increase one’s understanding of it. One way to examine these theories is with a ‘schools of thought’ approach that divides entrepreneurship into specific activities. These activities may be within a ‘macro view or a micro view’, but all address the conceptual nature of entrepreneurship.

Development of entrepreneurship

Creativity: Creativity and innovation are often used to mean the same thing, but each has a unique connotation. Creativity is the ability to bring something new into existence. Ideas usually evolve through a creative process whereby imaginative people bring them into existence, nurture them, and develop them successfully. The creative process for an idea contains five stages – germination, preparation, incubation, illumination, and verification.

8.1. Classification of essential nutrients

8.1.1. Based on the amount required by the plant

Major nutrients-Required in large quantities eg. N, P, K.

Secondary nutrients-Required in lesser quantities compared to major nutrients eg. Ca, Mg, S.

Micronutrients-Required in trace quantities eg. Fe, Mn, Zn, Cu, B, Mo.

The eyes and ears are not only vital sensory organs for your pet dog but also key indicators of their overall health and well-being. Proper care and management of these delicate areas are essential for preventing infections, maintaining comfort, and preserving your furry friend’s quality of life. Here’s a comprehensive guide to eye and ear care for pet dogs:

Eye Care

1. Regular Inspection

• Start by regularly inspecting your dog’s eyes for any signs of redness, swelling, discharge, or cloudiness. Clear, bright eyes indicate good health, while any changes may signal an underlying issue.

• Look out for excessive tearing, squinting, or rubbing of the eyes, as these may indicate irritation or injury.

Introduction

Public concern over use of antibiotics in livestock production has increased in recent years because of their possible contribution to emergence of antibiotic resistant bacteria, and their transmission from livestock to humans. Accordingly, ruminant microbiologists and nutritionists have been exploring alternative methods of favorably altering ruminal metabolism to improve feed efficiency and animal productivity. Plant extracts contain secondary metabolites, such as essential oils (EO) that have antimicrobial properties that make them potential alternatives to antibiotics to manipulate microbial activity in the rumen. Essential oils are naturally occurring volatile components responsible for giving plants and spices their characteristic essence and color. Over the last few years, a number of studies have examined effects of EO, and their active components, on rumen microbial fermentation. However, many of these studies are laboratory based (i.e., in vitro) and of a short-term nature. Nevertheless, results from in vitro batch culture studies provide evidence that EO and their components have the potential to improve N and/or energy utilization in ruminants. Effects of EO on ruminal N metabolism is more likely mediated by their impact on hyper-ammonia producing (HAP) bacteria resulting in reduced deamination of amino acids (AA) and production of ammonia N.

All higher plants have some kinds of aroma or flavor in their various parts, which includes roots, stems, leaves, flowers and fruits. This flavor and aroma in a particular plant part due to the presence of some specific essential oils present in special glands in it (Chadha and Choudhury, 1989). Essential oils arethe concentrated liquid, which is highly concentrated and water insoluble liquid (hydrophobic) and it is also known as ethereal or volatile oils, which are fragrant oily liquid that are extracted from the various parts of the plants. Generally they are organic compounds like hydrocarbons likealcohols, aldehydes, esters, ketones and phenols. An essential oil is “essential” in case that it contains the essence of the different fragrance, and the properties of the plants from which they are derived. All though all ornamental plants have some aroma but only few of them are used commercially in perfumes, flavor and cosmetic industries. The essential oil demand by fragrance industry is 60%, flavour industry 20% and pharmaceutical industry 20%. In all the perfumes, rose oil is one of the best and costliest perfume. The first recorded reference of rose perfume is in CharakSanghita. The credit for discovery of rose perfume was given to Queen Noor Jehan mentioned in the book written by Md. Achem of King Jahangir’s regime.

The volatile oils showed the different kinds of biological activities including the antibacterial, antioxidant, antiviral, insecticidal, etc. These oils are also used for cancer treatment, while some other has been used for the food preservations, aromatherapy, and in the perfumery industries. Thus, in the day to day life a large spectrum of usage the essential oil and aroma industry has a bright future. Yet the known applications of essential oilsare opening up ofmore areas which will benefit the industry.

The functional diversity of chemicals within plants is best demonstrated by terpenoids. More than 30,000 terpenoids have been identified, each one composed of 5-carbon (prenyl diphosphate) building blocks added together to make 10-carbon, 15-carbon, and even 2000–500,000-carbon chains, as is the case for rubber. Terpenes are functionally diverse, and many are integral to primary metabolism, e.g., hormones such as gibberellins and abscisic acid, electron carriers such as plastoquinone and ubiquinone, terpene-derived compounds that form structural parts of membranes such as phytosterols, and photosynthetic pigments such as carotenoids.

Plant terpenes are also available as essential oil or ethereal oil. Essential oil is defined as the volatile oil obtained by the steam distillation of plants. There is a distinction between fixed or fatty oils and volatile oils. Their volatility (boiling point) and plant origin are characteristic properties of these oils.

Terminology

    ·    Nutrient: Nutrients are substances required by an organism for their normal     growth and reproduction. 
    ·    Plant Nutrient: The plant nutrient is a “food” which is composed of certain chemical elements often referred to as ‘plant nutrient’ or plant food elements considered very essential for growth and development of plants. 
    ·    Nutrition: The supply and absorption of chemical compounds needed for growth and metabolism of an organism.

Agricultural meteorology, a discipline that has traditionally focused on understanding the relationships between weather and climate, and agricultural productivity, is at a critical juncture. The increasing vulnerability of agricultural systems to climate change, coupled with the need for sustainable and resilient agricultural practices, necessitates a fundamental transformation of agricultural meteorology. To support the development and implementation of climate resilient agriculture (CRA) practices, agricultural meteorology must be reoriented as a super speciality discipline. This requires a comprehensive overhaul of the discipline, including its curriculum, research focus, and practice. The reorientation of agricultural meteorology as a super speciality discipline is essential to provide the necessary expertise and knowledge to support CRA practices. This includes developing and applying cutting-edge technologies, such as precision agriculture, remote sensing, and machine learning, to support agricultural decision-making. However, the current state of agricultural meteorology is not equipped to meet the demands of CRA. The discipline faces several challenges, including a lack of interdisciplinary approaches, inadequate emphasis on practical skills, and limited focus on climate change and sustainability (Fig. 8.1). To address these challenges, essential reforms are necessary to reorient agricultural meteorology as a super speciality discipline. This chapter will discuss the essential reforms required to transform agricultural meteorology, including reforms in curriculum, research, practice, and policy. By implementing these

Introduction

An entrepreneur is someone who starts or runs their own business. By having an equity stake in the company, the entrepreneur can profit greatly if things go well; however, they also take on a great deal of risk-far more than a regular employee of the company. Financial, career, emotional, and overall business risks are all examples of entrepreneurial risk. Because there is so much at stake in starting and growing a successful business, there are very specific skills that an entrepreneur usually requires to be successful. It could be a picture of a talented college dropout or a seasoned business professional with an uncanny ability to predict the next big thing. Replace whoever the persona is with ourselves. A successful entrepreneur does not have a specific demographic or personality profile. We can be entrepreneurs regardless of our age, race, gender, sexual orientation, socioeconomic status, or upbringing if we have dedication, drive, and essential business skills. Unlike personality traits and demographic information, entrepreneurial skills can be learned and practiced. If we want to pursue entrepreneurship, use this list to assess our strengths and weaknesses and determine which skills we need to develop before launching our venture.

This chapter outlines fundamental molecular biology protocols essential for genomic analysis and molecular characterisation. Modern molecular biology relies heavily on precise analytical techniques for studying genetic material. Hence, this section presents a typical workflow encompassing nucleic acid extraction, amplification, and subsequent analysis. These methodologies are fundamental to various applications in molecular biology, including phylogenetic analysis, gene expression studies, and molecular diagnostics.
The chapter covers four core molecular biology and bioinformatics techniques, each building upon the previous:
1. Genomic DNA Extraction: Protocols for efficient isolation of DNA from biological samples. This encompasses cell lysis, protein denaturation, and nucleic acid purification through standard separation techniques. The quality and purity of extracted DNA significantly influences downstream applications.
2. Primer Design with Primer3: In silico design of oligonucleotide primers for PCR amplification. This section covers primer design parameters including optimal melting temperature (Tm), GC content, primer length, and considerations for avoiding secondary structures and primer-dimer formation.

At times, we hear our young generation questioning the need for obtaining degrees for conducting any research work. To some extend they are right. If we look back, there are many examples where persons without any former degree attained what is impossible by many degree holders. Tulsidas has written ‘Ramacharitmanas’, incorporating so many principles of Vedas and stories from Puranas without obtaining any degree. Srinivasa Ramanujan (21) narrated mathematics theorems without any degree in mathematics. His achievements may be appreciated by the fact that we celebrate his birthday 22nd December as National Mathematical day.

To set up a soil-testing laboratory the minimum requirements are as follows:

8.1 Laboratory floor area

Minimum four rooms are needed. They are as follows:

1. Office room, floor size: 18’x 12’

This room is used for registration of the soil samples received, recording recommendations along with soil test results in laboratory book and in report sheet, their dispatch, accounts etc.

The establishment of a technology transfer office is an important and complex process for an organization. It needs careful planning to ensure its long-term sustainability. Before establishing a TTO, it is essential to evaluate whether institution’s core mission includes research commercialization, and if the volume and quality of its research output justify the need for an inhouse TTO. For institutions that may not meet all criteria, those institutions can collaborate or outsource for TTO services. It is also important to decide about its operational scope, funding, management, and for what kind of issues the office should develop a policy. There is no “right” way to set up a TTO, but successful TTO does require considering some key issues. This chapter outlines key steps and considerations for establishing a TTO. It also explains what physical infrastructures are needed, manpower, outlines the responsibilities and powers of TTOs.
2.1 Evaluating the need for a TTO
Establishing a TTO is an important step for any research focused organization. But before initiating a planning process for a new TTO, the organization must review its relevance by considering a few fundamental points:
Key considerations for institutions
1. Is institution’s mission focused on “research commercialization” If an institute’s primary mission is education, or it does not support research it might not be justified to create a TTO. For such institutions, it usually makes better sense to seek alternative solutions to the occasional technology transfer without the expense of a formal office. But institutions with strong research commercialization focus, a TTO has the ability to contribute meaningfully to the mission. The Association of University Technology Managers (AUTM), USA has identified following key motivations for initiating academic technology transfer:

1. SEED TESTING

The science of evaluating the planting value of seed has been developed to achieve the following objectives for minimizing the risks of planting low quality seeds.

To determine the seed quality and its suitability for planting

To identify seed quality problems and their probable cause.

To determine the need for drying and processing, if any.

To determine if seed meets established quality standards for labeling.

To establish quality and provide a basis for price and consumer discrimination among lots in the market.

An orchard is the place where different kinds of fruit crops are grown in large scale. An orchard is a long-term investment and needs lot of planning and expertise. The orchard crops are generally trees, which ultimately have a large spread, so when they are planted, the distance between plants and rows is kept keeping in mind the final spread of the tree. So, before the trees attain their full growth, some suitable crops which are of short duration, like vegetables, flowers, spices and herbs are grown as intercrops.

Fruit trees are perennial, may be tropical, sub tropical and temperate types and thus require proper environmental conditions and suitable management practices for their successful growth.

Layout of an orchard

Layout means the way by which something is arranged or laid out. The purpose of designing a layout for an rchard is to assign the actual position for trees/ plantations, roads, water channels, buildings etc. The layout should be planned very carefully and implemented properly to facilitate proper care and maintenance of an orchard. The plan should provide optimum number of trees per unit area as well as sufficient space to each of the tree for proper development and ease in various cultural operations of the orchard.

Materials required for layout

Measuring tape, rope, pegs, poles and planting board.

Based on agro-climatic conditions, the following system of planting are generally followed for planting of fruit trees:

1. Square
2. Rectangular

Layout means the way by which something is arranged or laid out. The purpose of designing a layout for an rchard is to assign the actual position for trees/ plantations, roads, water channels, buildings etc. The layout should be planned very carefully and implemented properly to facilitate proper care and maintenance of an orchard.

ABSTRACT

Haploids are of great importance in breeding programmes for the production of homozygous diploid lines. This technique reduces the time span in developing new varieties and fixes the traits including recessive ones in the homozygous condition. The haploid embryos or doubled haploid plants could be used in mutation breeding, genetic engineering, biochemical and physiological studies. Isolated microspore culture for the production of haploids has not yet been reported in Hevea brasiliensis. Hence the present work was initiated with the view of producing haploid plants by the culture of isolated pollen grains. The flower bud of the clone RRII 105 at the late uninucleate to early binucleate stage was used for culture initiation. Different pretreatment solutions were tried at different temperatures (4°C, 27°C and 33°C) with the macronutrients of N6 media. Three kinds of callus induction media were used viz. N6, WPM and Hunters medium supplemented with myoinositol (200 mg^-1), glutamate (150 mg^-1), phenyl acetic acid (7.0 mg^-1), sucrose (70 g^-1) and growth regulators. Induction medium include moving liquid, static liquid and semi- solid medium. The ovary co-culture was also tried in the induction medium to increase the frequency of callus formation. Pretreatment of the anther with 0.3 M mannitol at 33°C promoted the division of microspores. Static liquid cultures improved the formation of multicellular microspores compared to semi-solid or moving liquid cultures. N₆ basal medium supplemented with 2.0 mg/l 2.4-D and 1.0 mg/l KIN induced microcalli formation. When the ovary co-culture was included in the callus induction medium, the rate of cell proliferation was increased considerably and the duration of callus formation was reduced from 3 months to 14 days. The results of the present study throws light towards the possibility of regenerating doubled haploids in future.

Selection of Site and Land Preparation

Cashew is a hardy plant; it thrives well on wide range of soil. While selecting the land for cashew, soils with water logging and excessive salinity of alkalinity should be avoided. Otherwise, soil depth, slope, stoniness, soil fertility and water availability seem to impose very little limitation. For establishing new plantations, land preparation should begin with the first pre-monsoon rains. The area should be cleared of bushes and other wild growth before digging pits for planting.

Planting Season

Planting of soft wood grafts is usually done at the beginning of monsoon season (June-July). Therefore, land preparation such as clearing of bushes and other wild growth, digging of pits for planting, should be done during pre monsoon season (April-May).

The concentration of essential elements must be maintained within quite narrow limits if the growth, health and productivity of the animal are to be maintained. Soil-plant-animal relationship is based on micro-mineral profile in animal blood, plant (fodder) and the soil is of paramount importance to identify dietary requirements of animals maintained under different husbandry practices of various agro-climatic zones. The soil occupies a strategic position in the chain of nutrition flow to plant, animal and eventually to man. The soil is the principal source of trace elements entering the food chain. The influence of the chemical composition of soil on the trace element contents of pant is well known.

1. INTRODUCTION

Field run seeds obtained from the seed production field posses high moisture and physical contaminants like trash and other inert materials (sand, stones, soil etc.,), diseased and pest damaged seeds, other crop seeds, weed seeds, deteriorated and damaged seeds, off-size and ill filled seeds, plant remnants etc. Seed processing is must to dry the seeds to safe moisture level, remove unwanted and inert materials as detailed above.

Seed qualities such as vigour and viability are improved by certain physical characteristics of the seed i.e., shape, size, large, denser and sound seed, etc. So, if grading is done to obtain a particular range of size, shape, length and density of the seeds, the quality of the lot is upgraded.

Seed processing refers to all the steps necessary for preparation of harvested seed for marketing namely handling, drying, shelling, preconditioning, cleaning, size grading, treating and packaging.

Extent of Passage
(Method of Krishna and Ekern, 1974D)
Definition of Terms
The term  extent of passage is used to describe how far a given ingested material travelled  or was allowed to pass. The point at which  undigested residue of the food comes to or has passed through the alimentary tract may be found by the use of markers without disturbing the subject. In some conditions of digestive system malfunction or disorder , the food residue may be blocked at point along with digestive tract.

Transit Time
This is the time it takes the digesta of a meal to pass through the alimentary tract or segments of it. This time also represents the  retention time in the tract or the particular segment. A simple way of calculating it is by recording the time of first or last appearance of the marked residue of a meal. Other more useful methods for calculating retention time include “mean retention time” (Castle 1956a), “mean time” (Blaxter et al., 1956) and “turnover time” (Hungate, 1968).

The damage to food grains in the storage may be caused due to insect attack, mould, fungi, bacteria, rodents, heat and moisture. The value of grain for milling purposes and human food is directly correlated with the amount of insect contamination present. The immature stages of rice and granary weevils, lesser grain borer, Angoumois grain moth and occasionally the flat grain beetle, cadelle, rust red flour beetle and saw toothed grain beetle live inside the kernels where they are hidden from view until they emerge.

Grain from casual inspection appears to be in good condition but may contain enough internal infestation which render it unfit for human food or unfit for purchase for future sale as milling stock. In general, millers have found that wheat with more than 0.5 per cent of infested kernels is unfit for milling. The need for a rapid and accurate method of estimating insect contamination in grain offered for sale has become more and more important to both grain dealers and millers.

Methods for Estimation of Damage

In order to assess the damage caused to food grains due to any one or combination of various factors; physical, chemical and micro-analytical methods are employed according to the necessity and information required. Various methods have been employed by field workers as well as by laboratory technicians in estimating damaged grains in food grains with particular reference to detection of insect infestation which alone cause maximum damage. These methods have been described below.

Principle

The portion of the total ash which is insoluble in dilute hydrochloric acid is called acid insoluble ash (AlA) and it is the part of ash which is having unavailable mineral matter like silica and sand. Silica is the part of mature plant materials which are highly lignified or woody like straw, husk, stems and stalks, while sand inters in feeds by faulty handling in the fields or as an adulterant.

Equipment/glass wares
    1.    Asbestos sheet
    2.     Filter paper (Whatman No.1)
    3.    Funnel stand
    4.    Glass funnel
    5.     Glass rod
    6.     Hot plate/heater/Bunsen flame
    7.     Metal tongs
    8.     Silica basin (crucible)
    9.     Volumetric flask
    10.     Wash bottle with distilled water

Nutritional significance of tannins in feeds and fodders

Tannins are widely distributed in many types of browse plants, tree leaves, forages and feed ingredients. The structure of tannins are not completely well defined but they are polymers of phenolic and aliphatic hydroxy compounds. Although tannins and related polyphenols are considered as protein binders and, protease and cellulolytic inhibitors, they can also exert beneficial effects on rumen fermentation. There are two forms of tannins viz., hydrolysable and condensed. Hydrolysable tannins are considered to have less deleterious effects on protein digestion since these types of tannins might get hydrolysed under acidic gastric condition and release bound proteins. Attempts have been made to utilize this to advantage in protection of proteins from rumen degradation.The condensed tannins are resistant to hydrolytic degradation. The reduction in digestibility due to condensed tannins is not clear whether it is due to specific interference with cellulases or precipitation of proteins.

Certain feed ingredients have been incorporated as a source of tannins, in the diets of ruminants to benefit ruminal fermentation and performance. Inclusion of Bengal gram husk as a source of tannins in the diet of sheep prevented potentially digestible carbohydrate in the rumen, but digested post ruminally (Sreerangaraju et al., 2000).

29.1. Aim

To estimate ascorbic acid in fresh and processed fruit and vegetable products.

29.2. Theory and Principle

Fruit, vegetables and their products are important sources of ascorbic acid. The ascorbic acid is present in sufficient quantity in aonla, guava, grapefruit, lemon, pineapple, strawberry fruits etc. Barbados cherry is the chief source of vitamin C. The products manufactured from these fruits are also considered as rich in ascorbic acid and the contents available in the commodities can be detected by using 2, 6 dichloro-phenol - indophenol visual titration method.

25.1. Aim

To estimate the ash content in fresh and processed products.

25.2. Theory and principle

Estimation of ash contents is important as it gives an idea about the presence of minerals in the food samples. Determination of ash is also required to evaluate fruit chutneys as the maximum limit for ash in this product shall not exceed 5% as per FPO specifications.

25.3. Apparatus/instruments/equipment

1. Crucibles

2. Muffle furnace

3. Sensitive balance

4. Heating plate

5. Desiccators

[Bray’s Method (Bray and Kurtz, 1945) – Acid Soils]

Introduction

The term available phosphorus (P) refers to the inorganic form, occurring in the soil solution, which is almost exclusively ‘orthophosphate’. This orthophosphate occurs in several forms and combinations, and only a small fraction of the total amount present may be available to plants, which is of direct relevance in assessing the P fertility level.

The phosphate concentration in solution is governed by heterogeneous equilibria in which it takes part. This situation can be represented as follows:

P adsorbed in solid phase ↔ P in the soil solution ↔ P precipitated

The phosphorus absorbed by plants from soil solution comes from the soil solution in which it exists as inorganic orthophosphate ions viz., H₂PO₄ ^–, HPO₄ ^2– and PO₄ ^3–. The most accessible ion is H₂PO₄ ^–, with the greatest activity coefficient, followed by HPO₄ ^2–. The quantity of P accessible to the plants is influenced by a series of soil properties. The relative abundance of these ions is, however, relatively dependent on the soil pH. For soils having a pH between 4.5 and 7.5, ions of H₂PO₄ ^– as well as HPO₄ ^2– exist in soil solution. At a pH of 7.2, H₂PO₄ ^–and HPO₄ ^2– ions have an equal activity, and, when the pH is strongly alkaline (>8.3), ions of HPO₄ ^2– predominate in solution. Above pH of 9.0, the trivalent ion (PO₄ ^3–) becomes more important than H₂PO₄ ^–, but even at a pH of 12, the HPO₄ ^2– concentration is still greater than that of PO₄ ^3–. Soluble phosphorus may be adsorptively retained at the surface of colloidal particles. This retention is more marked when the higher amounts of clay and sesquioxides are present. Relatively, the available inorganic P tends to accumulate in its most stable state under prevailing conditions, thus, in calcareous soils, the available inorganic P would be acid-soluble, whereas, in acid soils the adsorbed P would be more available. In flooded soils, with low oxidation potential, certain forms of P, normally considered as unavailable, for example, iron and phosphate, can be regarded as available. In most soils, the main source of orthophosphate is organic matter unless, of course, direct fertilization, with soluble phosphate, has been made.

(Neutral Normal Ammonium Acetate Method)

Introduction

The total potassium (K) content of a soil varies from 0.05 to 2.5 %. The total K is distributed in mineral form (lattice – K, 90-98 %), fixed non-exchangeable or temporarily retrograded K (1-10 %) and exchangeable plus water soluble K (1-2 %). Exchangeable K represents an average of 2 – 15 % of the sum of exchangeable bases and 1 – 3 % of the total capacity for cation exchange. The K concentration in the soil solution is on an average 0.08 – 3 meq/litre. Both water soluble and exchangeable K are most accessible to the plant. Available K can, thus be separated into that, immediately available, which is water soluble and exchangeable, and that, potentially available or fixed. The neutral normal ammonium acetate extract contains both water soluble and exchangeable K. K extraction by this extractant is considered as a suitable index of K availability in most soils, based on crop response correlation study. Thus, the K extracted by this method is equated as the available K.

[Williams and Steinbergs (1959) Method]

Introduction

Sulphur (S) occurs in numerous forms in soil, viz., sulphites, sulphates, sulphides and in organic compounds. It is considered that the most accessible form is ‘sulphate’ (SO₄ ^2–). The organic forms of S compounds become assimilable, especially following, microbiological transformation into SO₄ ^2–. The mineralization of organic S in a soil depends primarily upon the N:S ratio, and any SO₄ ^2– formed may be fixed against extraction, particularly if much Fe or Ba is present or if the soil is very acid. Despite the fact that the plants absorb S almost exclusively as SO₄ ^2–, mobility of SO₄ ^2– in soil may not always yield satisfactory results in accordance with the time of sampling, while assessing SO₄ ^2– availability.

Neem seed cake (NSC) despite of being rich in protein is not used as animal feed due to the presence of large number of toxic/bitter compounds in it. Studies have shown that NSC can be safely included the ration of animals after water washing / alkali/ urea treatment. These treatment methods appear to remove / reduce or largely inactivate the toxic principles present in NSC. In order to assess the quality/safety of the treated product, quantification of the residual toxic compounds is essential. One such compound is azadirachtin. It has been reported to be biologically most active.

One of the best techniques for the estimation of azadirachtin is by using high performance liquid chromatography (HPLC).

The bacterial load in the semen samples is estimated by standard plate count method. The bacterial contaminants of semen have been a major concern for most of the frozen semen production laboratories as it adversely affects semen quality and hence subsequent fertility. There exists a significant negative correlation of bacterial load and sperm livability, motility and in vitro fertility tests. Macrophages and polymorphonuclear granulocytes, which form the first line of defense against these microorganisms, produce reactive oxygen species (ROS) to kill these microorganisms. ROS, however, is also released outside these cells and may react with molecules and cells such as spermatozoa in their vicinity. The cellular antioxidants, present mostly in the cytoplasm, are scanty and inadequate to counteract this ROS as the sperm cell cytoplasm is very small (20 µm3) and mostly distributed in the midpiece. Therefore, semen samples with minimum bacterial load are expect to give better results on insemination.

Introduction

Sugars are commonly found in the form of biopolymers. Cellulose, a biopolymer of glucose is the predominant constituent of plant tissues, and may be shortly described as the structural bases of vegetable world. In this case, the glucose moieties are strung together by P-glycosidase linkages, which form the linear chain, making it resistant to chemical attack. Hemicellulose consists of short, highly branched, chains of sugars. It contains five carbon sugars (usually D-Xylose and L-Arabinose) and six carbon sugars (D-Galactose, D-Glucose and D-Mannose) and uronic acid. Its branched nature renders hemicellulose amorphous and relatively easy to hydrolyse to its constituent sugars. Lignin is a biopolymer rich in phenolic components, which provide structural integrity to plants. Acid and neutral detergent fraction methods (ADF and NDF) involve the gravimetric determination of residue previously treated with acid and neutral detergent solutions. These methods determine the insoluble fraction and their individual components. NDF represents the total plant fibre or cell wall including hemicellulose

1. Introduction

Insects, like many other organisms, rely heavily on their diet for essential nutrients that regulate their growth and development. The quality of food intake significantly impacts various aspects of an insect’s life cycle. Research, such as that conducted by Lee (2015) highlights that consuming a diet lacking in essential nutrients can lead to premature aging, a process known as senescence, in insects. Furthermore, such inadequate nutrition can hasten the rate of mortality as the insect ages. This underscores the critical role that proper nutrition plays in the longevity and overall health of insect populations.

Carbohydrates are constituents of all living cells. Animal as well as plant cells use carbohydrates as source of energy. Blood glucose is a useful indicator of energy metabolism and fertility status. Under certain clinical circumstances, estimation of carbohydrates becomes essential. Sugar levels (particularly glucose) in blood or urine are used as an important parameter for diagnosis of ketosis and diabetes mellitus.

Urea is the detoxification product of the ammonia derived from the deamination of amino acids. Urea is therefore the most common nitrogen-containing end product of protein catabolism. Its synthesis from ammonia occurs in the liver (via the urea cycle), and it is then excreted by the kidneys. The concentration of urea inside red cells is slightly less than in plasma due to the presence of large amounts of hemoglobin inside the cells. Whole blood urea concentration is therefore slightly less than plasma (or serum) urea. Therefore, usually serum is used instead of whole blood for the determination of urea. The familiar term BUN (blood urea nitrogen) persists in spite of the fact that it is serum urea nitrogen which is usually measured.

Estimation of calcium and phosphorus in feeds: Calcium is precipitated as calcium oxalate by adding ammonium oxalate to acid soluble extract prepared after running a sample for ash. Precipitates of calcium oxalate are dissolved in sulfuric acid and titrated with potassium permanganate to know the content of calcium.
Using N1V1 ≡ N2V2, 1.0 ml of 0.1 KMnO4 = 2.004 mg Calcium.
Calcium, g % = Burette reading difference X 2.004 X dilution factor X 100 / weight of the sample used for preparation of acid soluble mineral extract X 1000.
[dilution factor, (for example) = 250 / 25 (ml aliquot taken) = 10]
Phosphorous present in the sample is precipitated with ammonium molybdate and the precipitates are dissolved in NaOH solution. Excess sodium hydroxide is titrated with nitric acid to calculate the actual volume of NaOH used up which is direct proportion to the Phosphorous content.
1 ml of 0.1 N NaOH = 0.1347 mg Phosphorus
Phosphorus % = Actual volume of 0.1N NaOH solution used to dissolve precipitate X 0.1347 X dilution factor X100 / weight of sample used for acid soluble extract X 1000
[Here, dilution factor is 250 / 50 (aliquot taken) = 5]

Principle

When a solution containing calcium is treated with ammonium oxalate, all the calcium present is precipitated as calcium oxalate. The precipitate when dissolves in sulphuric acid, forming calcium sulphate and liberating free oxalic acid which is quantitatively estimated by titration against standard N/10 KMnO₄ to arrive at the calcium content present in the given solution. Following chemical reactions takes place in the estimation of calcium: