eChapter Name: Exploring the Biofuel Production Through Algal Genetic Engineering: Current Techniques and Challenges
9789372196429
eBook Name: BIOFUELS PRODUCTION USING SUSTAINABLE BIOPROCESSING TECHNOLOGIES
1. Background
The growing global population, industrialization, and urbanization have driven rising energy demand, with primary energy use increasing by 5.8% in 2024 (BP Statistical Review of World Energy 2024). The need for secure, affordable, and low-carbon energy is critical due to fossil fuel depletion, global warming, and geopolitical tensions. Clean energy sources like solar, wind, and biofuels are being explored, with biodiesel standing out as the only biofuel capable of fully replacing fossil fuels (Aizouq et al., 2020). Many countries, including India and China, are advancing biofuel production, with the latter viewing it as a key to energy security. Biofuels, derived from various sources like algae, crops, and waste, include biodiesel, bioethanol, and biogas. They are categorized into four generations based on feedstock. First-generation biofuels, made from edible materials, face food vs. fuel competition. Second-generation biofuels from non-edible sources are more sustainable but still face resource limitations (Lv et al., 2019). Thirdand fourth-generation biofuels from microalgae offer higher yields and better land use, with species like Chlorella vulgaris and Nannochloropsis oceanica showing promise for biodiesel production. Microalgae’s fast growth and efficiency have led to advances in biodiesel production, although challenges remain, such as species selection and production scale-up (Arora et al., 2021). But there is a constraint for commercial availability of these biofuel which are the economic and environmental obstacles that are creating problematic condition for approval and use of genetically engineered microalgae for their potential to enhance lipid yields. This chapter delves into the current opportunities and challenges in microalgae biofuel production, focusing the need for regular research and innovation (Maliha and Abu-Hijleh 2022).
2. Choosing the Different Classes Of Microalgal Strains
The achievement of huge-scale microalgal culture relies on picking appropriate species. The production of various lipids greatly affects the selection of strains and biofuel properties. For the biodiesel, Triacylglycerol (TAG) is the preferred lipid but compared to green algae its content is typically low in cyanobacteria. with the average lipid content of 25.5%, green algae (Chlorophyceae) stand as the largest class of oleaginous microalgae, which can rise under nutrient stress (Saini et al., 2021). Also, the biomass productivity is equally important for high lipid productivity as high lipid content is beneficial. For biodiesel production, strain selection should focus on both lipid content and biomass. Chromochloris zofingiensis shows the highest lipid productivity among all classes of green algae. The saturated (SFA) and monounsaturated fatty acids (MUFA) fatty acid (FA) being dominant, their composition also plays a vital role in biodiesel quality. It has seen those high levels of C16:0 and C18:1 in microalgae is ideal for biodiesel production (Bharti et al., 2021). Therefore, for improved biodiesel performance, strain selection should consider both lipid productivity and FA composition (Bagchi and Mallick, 2016).