eChapters

Introduction

While experimenting with chemicals, sometimes we need to dry the chemical/ solution (solid, liquid or gas) using a process like evaporation or distillation, mainly to remove water or different solvents. Drying is the process of evaporation or distillation to remove water/solvent from a solution, solid or even from gas. Generally, a drying agent (usually an anhydrous inorganic salt) is used for this process, which reacts with the water (present in the chemical/ solvent to be dried) to form a hydrate. For example, anhydrous MgSO4 is one of the drying agents. The drying agents are recognized by their capacity (the rate at which they absorb water) and their intensity (the amount of water left behind in the solvent at equilibrium). No chemical reaction or change in phase occurs during the drying process.

Type of Solvents

Apart from water (a universal solvent), there are three families of organic solvents used in laboratories.

1) Oxygenated solvents: Alcohols, Ketones, Glycol ethers, Glycol esters, etc.

2) Hydrocarbon solvents: Aliphatic and Aromatic hydrocarbons.

3) Halogenated solvents: Chlorinated hydrocarbons.

Introduction

For agricultural applications as well as for research work, often we need to prepare solutions of different agrochemicals of varying strength, as per the requirements. Agrochemicals are used to help crop plants grow, produce, and protect them from biotic/abiotic stresses. Many of the agrochemicals (insecticides, herbicides, fungicides, rodenticides, molluscicides, nematicides, etc.) are used to protect the crop from insect pests and diseases. However, some of them (fertilizers and soil conditioners) are used to augment crop yield. While in the previous chapter (Chapter 8) we discussed about preparation of laboratory solutions, in this chapter we will briefly discuss about the preparation of different agrochemicals.

Agrochemical/agrichemical is a generalized term used for the chemicals applied in agriculture. In general, it also includes the feed-supplements used in f isheries and animal husbandry.

Agrochemicals

Fertilizers

Fertilizers are used to augment growth of crop plants and to mitigate nutrient deficiency in the soil. Fertilisers can be organic (containing natural substances, prepared through natural processes) and inorganic fertilisers (synthetic fertilizers, manufactured artificially using chemical processes using natural deposits, by chemical alterations).

Soil Conditioners

Soil conditioners include manure, livestock and leaf compost, peat that help making the soil in good condition. They are laid on the top soil and then mixed properly. They not only increase nutrient contents in the soil but also improve aeration and water holding capacity.

AMR has been prevalent in almost all European countries from past decades. A 2021-2022 report published by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC) on AMR in zoonotic and indicator bacteria from humans, animals and food origin reported vast differences in antimicrobials resistance levels among the European countries, with comparatively higher AMR percentages in the southern and eastern parts of Europe (ECDC, 2022, 2023). The resistance levels ranged from very high for tetracyclines, sulfonamides and ampicillin, moderate for ciprofloxacin to low levels for third-generation cephalosporins (cefotaxime and ceftazidime) in human cases infected with Salmonella spp. in 2022 (ECDC, 2023). The trends are dynamic and over a decade of 2013 2022, the human isolates from 12 and 15 countries reported a statistically significant reduction in tetracycline and ampicillin resistance, particularly for S. typhimurium (ECDC, 2023). The ciprofloxacin and cefotaxime combination, a highest priority critically important antimicrobials (CIA), had very low and rare resistance levels for Salmonella isolates of humans and animal and their derived meat.

Livestock species wise variation was also observed in the resistance pattern in imported meat, being higher in poultry meat isolates compared to pig or cattle meat for Escherichia coli (ECDC, 2023). High to extremely high levels of f luoroquinolone resistance from Campylobacter jejuni and C. coli of both human and animal origin isolates exist, due to which the fluoroquinolones can no more be used as a treatment option for human Campylobacter infections (ECDC 2023). The combined occurrence of erythromycin resistance and ciprofloxacin in Campylobacter spp is considered of high public health importance, and the report mentioned that the combined resistance for these antimicrobials was higher in C. coli compared to C. jejuni from food-producing animals and humans. However, while the ciprofloxacin resistance showed an increase in over 13 study countries, seven countries reported reduced erythromycin resistance for C. jejuni. Overall, resistance to conventional antimicrobials was frequent in Campylobacter and Salmonella from humans and animals. Increasing trend in resistance to one of two CIA for treatment in humans has been observed in poultry-originated Salmonella serovars and Campylobacter while combined resistance was is low in few Salmonella serovars and C. coli isolates from humans and animals in some countries (ECDC, 2023).

Introduction

Until the 20thcentury, bacterial infections were the number one cause of human death. In the 1940s, antibiotics became the lifelines for treating serious infections that saved millions of lives. Despite the advantages derived from the use of antibiotics, resistance to these drugs emerged. Unfortunately, resistance to most antibiotics emerged in time-course even for the most powerful antibiotics developed.Resistance to antibiotics is a challenge to the scientific community in keeping up the pace of antibiotic discovery.World Economic Forum’s Global Risks Reporthas listed antibiotic resistance as one of the greatest threats (WEF, 2020).United Nations (UN) has warned that drug-resistant diseases could account for 10 million deaths each year by 2050. By 2030, antimicrobial resistance (AMR) could likelylead up to 24 million people into extreme poverty(IACG,2019). Currently, at least 7,00,000 people die each year due to drug-resistant diseases (IACG,2019). The emergence and widespread of antibiotic-resistant organisms is a global challenge and needs a multi-dimensional approach to address the fundamental issues to minimize drug-resistance.The Antibiotic Resistance Genes Database (ARDB) contains a repository of around 23,000 antibiotic resistance gene or protein sequences conferring resistance against over 240 antibiotics (ARDB). Computational Biology (Bioinformatics) has become one of the important tools in studying antimicrobial resistance. It employs a range of computation techniques to make predictions about the biological phenomenon.

Introduction

The epidemiological risk and hazards to global health security posed by AMR has been reiterating in several World Health Assembly (WHA) declarations and has been prioritized under the Global Health Security Agenda (GHSA). The Indian Ministry of Health & Family Welfare (MoHFW) has also identified AMR as one of the top priorities for collaborative work with WHO and the first National Action Plan on AMR has been implemented (2017-2022). India ranked fifth in antibiotic consumption in food producing animals (ruminants, pigs, and poultry) in 2010. Even though there has been a gradual reduction of 3.6% in antibiotic consumption during 2011-2019 (Koya et al., 2022), with mounting incomes and shifting in dietary patterns in favour of the consumption of animal protein, especially for poultry, the veterinary antibiotic utilization is projected to further rise by 312%, elevating India to the fourth rank by 2030 (Van Boeckel et al., 2015). FAO has already reported some of the peak resistance rates among common commensal and environmental bacteria against antibiotics like penicillin, cephalosporins, erythromycin and co-trimazoles in India (INFAAR). Resistance to the broad spectrum fluoroquinolones and third generation cephalosporin antibiotics is already more than 70% in Escherichia coli, Klebsiella pneumoniae, and, Acinetobacter baumannii and more than 50% in Pseudomonas aeruginosa. Even the microbial resistance to carbapenems, the last-resort antibiotics, is extremely high among gram-negative microorganisms (Sumanth et al., 2016). To overcome these incidences, it is impulsive to understand the microbe and drug interaction in host system.

Introduction

Malnutrition remains a pervasive global challenge, affecting millions of individuals across the world, particularly in low- and middle-income countries. Despite significant advancements in agriculture and food production, a large segment of the population still lacks access to essential nutrients, leading to severe health consequences and perpetuating cycles of poverty and underdevelopment (Müller and Krawinkel, 2005). Malnutrition, defined as the inadequate intake of essential nutrients required for optimal health and development, encompasses both undernutrition and over-nutrition (WHO, 2023). While undernutrition, characterized by deficiencies in energy, protein, vitamins, and minerals, remains a significant concern, over nutrition, manifesting as obesity and diet-related non-communicable diseases, is also on the rise, particularly in urban settings (Wells et al., 2020). The consequences of malnutrition are far-reaching, impacting physical growth, cognitive development, immune function, and overall well-being, with long term implications for productivity, economic prosperity, and societal stability (Saunders and Smith, 2010).

Rice, a staple food for more than half of the world’s population, plays a central role in global food security and nutrition. However, conventional rice varieties often lack sufficient levels of key nutrients, such as iron, zinc, vitamin A, and certain amino acids, leaving populations reliant on rice-based diets vulnerable to micronutrient deficiencies and associated health risks (Peña-Rosas and Mithra, 2019). Iron deficiency anaemia, zinc deficiency, and vitamin A deficiency, are among the most prevalent forms of malnutrition worldwide, particularly affecting women and children in resource-constrained settings where rice consumption is high (Dipti et al., 2012)

Significant Progress and Prospects

It is widely acknowledged that biofortification presents a promising and cost effective agricultural strategy to enhance the nutritional status of malnourished populations globally. Approaches to biofortification, including crop breeding, targeted genetic modification, and mineral fertilizer application, offer significant potential for combatting mineral deficiencies in human diets. The development of biofortified food crops with enhanced nutrient content such as iron, zinc, selenium, and provitamin A has shown promise in addressing micronutrient deficiencies prevalent in both developing and developed nations. Over the past decade, both national and international initiatives have made substantial contributions toward achieving these goals. These efforts have resulted in the release of biofortified varieties across various crops, including rice, maize, wheat, potatoes, vegetables, and millets. The majority of these varieties have been developed through conventional breeding methods without sacrificing crop yield, making them readily accepted by both growers and consumers.

One of the United Nations’ sustainable development goals is the cultivation of nutritionally rich crop varieties with heightened levels of micronutrients such as iron, zinc, calcium, total protein, lysine, tryptophan, anthocyanin, provitamin A, and oleic acid, coupled with reduced levels of anti-nutritional factors. In the past decade, 142 biofortified varieties, spanning rice, wheat, maize, pearl millet, small millet, lentil, chickpea, and other crops, have been developed under the auspices of the Indian Council of Agricultural Research (ICAR). Biofortified varieties typically do not impact ecological conditions, soil, or water requirements differently from traditional varieties. Additionally, they do not incur extra cultivation costs, and their economic output is comparable to traditional produce, leading to their widespread adoption. In India, the scale-up of biofortified varieties has gained momentum, with substantial quantities of breeder seeds being produced and distributed to public and private seed agencies for further multiplication and dissemination to farmers. Over the past six years, approximately 10 million hectares of land have been cultivated with biofortified rice, wheat, pearl millet, mustard, and lentil varieties.

Introduction

Millet is a very important cereal grain that many people around the world eat. In fact, over a third of the world’s population consumes millet. It’s the sixth most produced cereal crop globally. Some common types of millet include Jowar (Sorghum), Sama (Little millet), Ragi (Finger millet), Korra (Foxtail millet), and Variga (Proso millet). Among these, Bajra and Sama have more fat, while Ragi has the least. Millet has been cultivated for thousands of years and is used in many parts of the world. In the past, even the Romans and Gauls enjoyed millet-based porridges. Today, China, India, Greece, Egypt, and various African countries are the major producers of millet. In rural areas, some types of millet, like Finger millet and Sorghum, are eaten by people, while others are primarily used as animal feed. These millets have packed with several beneficial nutrients and play a crucial role in traditional diets in many regions. Compared to commonly used rice and wheat, millet is three to five times more nutritious. While wheat and rice mainly provide food security, millet offers not only food security but also health benefits and livelihood opportunities. It can help manage health issues like diabetes and high cholesterol.

In India, Karnataka is the top producer of millets, accounting for over 58% of global production. Despite its nutritional and health benefits, many people in India are not aware of the advantages of consuming millet. (Upadhyaya et al., 2007). Millets are fantastic crops when it comes to pest management. Traditionally grown millets don’t require pesticides and the land used for millet cultivation remains largely pest-free. Some millets, like foxtail millet, not only resist pests themselves but also serve as natural protectors against pests in storage conditions for other crops like green gram. This means there’s no need for fumigants to keep them safe. It has a treasure trove of micronutrients, including vitamins and beta-carotene, which are now seen as essential as pharmaceutical pills. In the present day, millets are exceptionally superior and serve as a solution to the widespread issues of malnutrition and obesity affecting a significant portion of the Indian population (Singh KP et al., 2012). Millets are rich in essential fatty acids, including linoleic, oleic, and palmitic acids, which are present in their free form. They also contain compounds like monogalactosyl, diacylglycerols, digalactosyl diacylglycerols, phosphatidylethanolamine, phosphatidylserine and phosphatidylcholine in bound forms. Although other fatty acids like arachidic acid, behenic acid and erucic acid are found in millets, they are in trace amounts. Millet oil can be a valuable source of linoleic acid and tocopherols. Additionally, millets are gluten-free and have an alkaline effect in the body. They contain essential B vitamins such as niacin, folacin, riboflavin, and thiamine, as well as phosphorus. These nutrients play a crucial role in energy synthesis within the body.

Introduction

The term analytics and data analytics are used inter-changeably. When referring to analytics, it means “analysis of data” or “what can be learnt from an analysis of data”. In an industry like fishing industry, the researchers have been slow to adopt the tools used to portray accurate representations of data. For example, a dashboard with historical data from the past ten or fifteen years displayed on a digital map can enable the researchers to decide the places where fishing can be good. This map can help in locating the points fished during specific seasons. This will enable the researchers to filter the data on various parameters like area, year, season etc. or on any other specific parameter which can give valuable information. Similarly, this type of dashboard can affect the decision making process while fishing, like one can find the monetary value associated with each trip during a specific season or compare the values associated with two different trips to gauge the cost-benefits.

Data Analytics helps in taking most informed decisions in the fishing industry if one of the goals is to increase income while ensuring sustainability. It helps in deciding whether to reduce overhead costs or to increase expenditure to avoid overhead costs in future.

Fisheries sector undergoes continuous changes with introduction of newer technologies evolved through research and development institutions. Statistics per se deals with generation of data, data management, data analysis and information generation from data. There are varied disciplines of fisheries viz., Aquaculture, Fisheries Resource Management, Fish Genetics, Fish Biotechnology, Aquatic Health, Nutrition, Environment, Fish Physiology, and Post-harvest Technology, wherein research on practical aspects is undertaken which affect production and sustainability.

Introduction

Indian agriculture, including fisheries, is highly monsoon dependent and effect of climate change is immense on its large tracts of rainfed farms. The effects of climate change could be thwarted through better adaptation techniques using all information available with various agencies concerned with agriculture and using advanced analytical tools. Presently, in the country, there is a gap in the capability in capturing, processing and analyzing huge source of information generated in Indian agriculture. This task can be accomplished using Big Data Platform which works in cluster mode and provides the capability of handling huge volume of data, capturing streaming data or real time data and also can accommodate unstructured data. As per FAO, 70 percent in increase in food production in another 30 years is required to overcome the increasing global food demand. It may not be possible to achieve that target due to variety of problems such as reduction in land for cultivation, climate change, and various other factors, such as increasing the fish production etc. Improvement in crop genetics, application of fertilizer in higher dosages, irrigation facilities, etc. may not be possible in future. We need to create a big data platform involving important parameters on crop production viz., soil, soil temperature, water requirements, and relative humidity, pests and diseases, and micro and macro nutrients in temporal and spatial. Now, since available data is properly utilized for making valid analytics by the farming community due to certain issues. Even the data created is incompatible, data available from various sources is also not compatible among themselves due to which decision making is also a big challenge. Due to the incompatibility of agricultural data from different sources, farmers do not have the accessibility to vast pool of available information for making effective, efficient and timely decisions.

Big Data in Agriculture (including fisheries)

Big data, an emerging data driven system helps in precision agriculture based on data collected. This helps farmers in making efficient decisions on sowing, planting, fertilizing and various other farm operations including

harvesting crops at the appropriate time. Of late, sensors are placed in the fields to collect data on related parameters viz., soil temperature and moisture relative humidity, wind speed, weather, etc. which help in crop growth. Images obtained using satellites are helpful in monitoring the crop growth during crop period. Agro-produce includes not only yields obtained from crops but also include produce from horticulture, livestock, fisheries, and other systems also. Agriculture is dependent on various parameters such as climate, seed, soil cultivation practices, agronomic practices, irrigation requirement, fertilizers pesticides, weeds, harvesting, post harvesting techniques, etc. Data collected on all these parameters would help in estimation of yield and other relevant parameters. Governments, Universities, Research departments, Agro-business and Agri-input companies generate, maintain and use voluminous data related to various farm management practices and other related issues say insurance marketing, supply chain, packaging, distribution, etc. This voluminous and wide variety of data poses several challenges for making effective decisions. Information on various fish related parameters can be collected from the stakeholders and a big data platform can be developed for effective decision making.

Introduction

Resources are used unscientifically commonly. For example applying feed somewhere in the pond results in loss, but by using technology, we can know when to apply where to apply, and how to apply which leads to efficient management of resources. Monitoring should be continuous which is not possible and very difficult for humans, but with technology, it could be monitored and the status could be known even from home which is communicated through mobile, which is sent by sensors installed. The data are also easily maintained. When technology is used, resources are efficiently utilized, since everything has a demand and that can be satisfied. So resources can easily be maintained which reduces wastage. Field management is a very skilled activity and it needs years of experience and onsite exposure which needs skilled labour, in such cases technologies aids with better methodologies. When the resources are applied at the right time, at the right amount, the growth becomes normal which increases the produce quality. After a product is produced, it has to be sent to a series of channels such as transport, market etc. and a technology should backup and monitor everything. The produce after being produced should be sold and many apps can be downloaded to improve the marketing. The whole world is changing within a period of time. So one should stay updated to impress the livelihood and the wide knowledge on technology helps to lead a better life with easy management practices. Thus data driven technology interventions are needed for efficient management of resources, monitoring of things anytime & anywhere, reduction of wastage of resources, reduction of dependence on trained and skilled manpower, enhanced product quality, better integration, better marketability, to stay updated with latest knowledge and access to other knowledge source for better upgrades.

Introduction

Cell surface components such as adhesins, polysaccharides, and proteins play major roles in the adherence of probiotic bacteria to the intestinal epithelium, interactions that might lead to pathogen exclusion and immunomodulation of host cells. Probiotic bacteria have the ability to produce exocellular polymers called Exopolysachcharides (EPSs). These exocellular polymers present in the surface of many lactic acid bacteria, including Lactobacilli.The adhesive properties of lactobacilli are directly linked to their surface properties which are influenced by the structure and composition of their cell wall. The cell envelope of lactobacilli, bacteria, is composed of the bilipidic plasma membrane with embedded proteins encompassed by the cell wall. The bacterial cell wall consists of a thick multilayered sacculus made of peptidoglycan (PG) with teichoic acids (wall teichoic acids (WTA) and/or lipoteichoic acids (LTA)), exopolysaccharides (EPS), proteinaceous filaments called pili, and proteins that are anchored to the cell wall through different mechanisms (Figure 1).

Some species of lactobacilli possess an additional paracrystalline layer of proteins surrounding the PG layer, referred to as the S layer. These macromolecules together may play crucial roles in determining species and strain-specific characteristics of lactobacilli by influencing host-microbe interactions and microbial adaptations to the changing host environment. Several studies implicate that cell surface components, either individually or collectively, involve in host-microbe interactions. Lactobacilli beneficially affect the host in many ways by producing abundant variety of EPSs through the activity of extracellular glycosyl transferases. Approximately 30 species of lactobacilli are described as EPS producers. Among them, the best known are L. casei, L. acidophilus, L.brevis, L. curvatus, L. delbrueckii bulgaricus, L. helveticus, L. rhamnosus, L. plantarum, L. johnsonii, etc.

The salts of the milk include those constituents that are present as ions or in equilibrium with ions. The main salts of milk are phosphates, citrates, chlorides, sulfates, carbonates, and bicarbonates of sodium, potassium, calcium, and magnesium. Approximately 20 other elements are found in milk in trace quantities including copper, iron, lead, boron, manganese, zinc, iodine, etc. These ions are more or less associated with themselves and with proteins. Some metals act as catalysts in the oxidation of milk fat leading to oxidative rancidity and certain metals like Ca, P, Mg, Mo, Co, Fe, etc. are components of metalloproteins such as lactoferrin, lactoperoxidase, xanthine oxidase, and parts of phospholipids.

Distribution of Salts Between Casein Micelles and Serum

The phosphorus is present as orthophosphate, but part of it is bound to organic components like serine and threonine residues of casein, molecules of hexoses and glycerol, phospholipids, etc. Phosphorus is also found in the hydroxyapatite structure of the colloidal calcium phosphate in the micellar structure of casein. The sulfur content of milk is about 0.36 g/kg, but most of it is in the amino acid residues methionine and cysteine of the proteins. About 10% is present as inorganic sulfate.

Physical Equilibria Among Salts

Milk contains several elements but all of them are not entirely in a soluble state. Some minerals exist in colloidal and ionic states at the normal pH of milk. The compounds that are in soluble condition play an important role in keeping various milk constituents in stable condition. A balance exists between the components that are in a soluble state and those which are in colloidal state.

Types of Enazymes in milk

1. Endogenous and 2. Exogenous.

Three sources of enzyme in milk:

1. The blood via defective mammary cells.

2. Secretory cell cytoplasm. Some of which are occasionally entrapped with fat globules by the encircling MFGM.

3. The outer layer of MFGM is probably the principal source of endogenous enzymes in milk. The outer layer of MFGM is derived from the apical membrane of the secretory cell which in turn originates from the Golgi membrane and acts as the principal source of enzyme in milk.

General Characteristics of Milk Enzymes

1. Deteriorative character e.g. lipase (commercially most important enzyme in milk), proteinase, acid phosphatase, xanthine oxidase.

2. Preservative enzymes. e.g. superoxide dismutase, sulfhydryl oxidase.

3. As an indicator of the thermal treatment history of milk. Related enzymes are alkaline phosphatase, ϒ glutamyl transpeptidase, and lactoperoxidase.

4. Mastitis infection indicator. Related enzymes are catalase, n-Acetyl-β D-glucosaminidase, and acid phosphatase.

5. Antimicrobial activity.

6. Commercial source of ribonuclease and lactoperoxidase

Common Milk Enzymes and their Role in Dairy Industry

Chemistry of Creaming

After being drawn from animals, normal milk will form a cream layer on standing. Because of the difference in density between fat globules (920 kg m−3 at room temperature) and milk plasma (density 1030 kg m−3), the granules tend to rise. This causes creaming, which we often want to prevent and it enables milk to be separated into cream and skim milk. Creaming is much enhanced when the globules have been aggregated into flocculates, clusters, or granules.

Cream Ripening

This unit operation serves as an intensive preparation of the cream for the churning process. It is divided into two categories.

1. Physical ripening;

2. biochemical ripening The following criteria are influenced by the cream ripening:

• Consistency, firmness, and spreadability of butter.

• Basic water content of butter during the manufacturing process (bound water in butter, which cannot be removed by any mechanical treatment).

• Buttermilk fat content, which has to be minimized.

The melting and solidification behavior of fat depends on the attached fatty acid, which indicate the hardness of the butterfat.

Natural hardness is caused mainly by seasonal feeding of the milk animals. Winter feed with a great deal of dry feed or roughage yields a hard butterfat with a high percentage of saturated and short-chain fatty acids and few double bonds in the fat molecules.

Summer feeding (green grass) results in fat with longer chains and a higher percentage of unsaturated fatty acids with many double bonds. We therefore distinguish between summer fat and winter fat or summer cream and winter cream.

Concentrated milk products are obtained through partial removal of water which increases the dry matter content of milk. The increase in dry matter content in concentrated milk is proportional to the degree of concentration. The milk concentrate may be prepared from either full-cream whole milk or fat-free skim milk.

Depending on the nature of raw material and other non-milk additives concentrated milks are classified into four broad categories.

1. Sweetened condensed milk

2. Evaporated milk or unsweetened condensed milk

3. Sterile milk concentrate

4. Reconstituted concentrated milk

Sweetened Condensed Milk

One of the earliest dairy products to be produced industrially is sweetened condensed milk. According to FSSA (2006), sweetened condensed milk is the product obtained by partial removal of water from the milk of cow/buffalo with the addition of sugar or a combination of sucrose with other sugars or by any other process that leads to a product of the same composition and characteristics. The fat and/or protein content of the milk may be adjusted by the addition and/or withdrawal of milk constituents in such a way as not to alter the whey protein to casein ratio of the milk being adjusted. It shall have a pleasant taste and flavor and be free from off-flavor and rancidity. It shall be free from any substance foreign to milk. It may contain food additives. It shall conform to the microbiological requirement as laid down by FSSA. The said product shall also conform to the following requirements

Commercially, one of the most significant milk products is ice cream. It is a frozen product which is usually made from milk or cream that has been f lavored with fruits like peaches or strawberries, and spices like vanilla or cocoa, or with sugar or a substitute. Stabilizers are sometimes combined with food coloring. Ice cream mix typically contains 10.0% milk fat, 11.0% solids not-fat, 14.0% added sugar, and 0.3% additives like an emulsifier, stabilizer, f lavor, and color substances.

Standard of Ice Cream

According to FSSA (2006), ice cream, kulfi, chocolate ice cream or softy ice cream means the product obtained by freezing pasteurized milk prepared from milk and/or other products derived from milk with the addition of sweetening agents and fruit products, egg products, coffee, cocoa, ginger, and nuts. It may also contain chocolate, and bakery products such as cake or cookies as a separate layer and/or coating. It may be frozen hard or frozen to a soft consistency. It shall be free from artificial sweeteners. It shall have a pleasant taste and smell free from flavor and rancidity. It may contain food additives. It shall conform to the microbiological requirements as given in FSSA (2006).

Food adulteration is a global issue, and because there are insufficient regulations and oversight, developing nations are more vulnerable to it. But in many nations, food adulteration has gone unnoticed. Contrary to popular assumption, milk adulterants can, regrettably, present major health risks that result in fatal diseases. Modern milk adulteration involves increasingly complex techniques, necessitating state- of- the- art research to identify the adulterants. Because milk contains so many nutrients that are needed by both adults and infants, it is referred to as the ideal food. In terms of protein, fat, carbohydrates, vitamins, and minerals, it is among the best sources. Regretfully, milk adulteration is a very common problem worldwide. Potential causes could be the gap between supply and demand, the perishable nature of milk, the low purchasing power of the consumer, and the absence of appropriate detection tests. Because there is insufficient oversight and inadequate law enforcement, the situation is substantially worse in developing and underdeveloped nations. While quantitative detections of adulterants in milk are varied and complex, qualitative detections can be carried out simply using chemical reactions. The type of quantitative detection methods used depends on the milk’s adulterants.

Typical Adulterants in Milk

The addition of vegetable protein, milk from different species, whey addition, and watering are the main adulterants in milk that are known to be economically motivated. There is no serious health risk associated with these adulterations. Some adulterants, though, are too dangerous to ignore. Urea, formalin, detergents, ammonium sulfate, boric acid, caustic soda, benzoic acid, salicylic acid, hydrogen peroxide, sugars, and melamine are a few of the main adulterants in milk that have been shown to have serious negative health effects.

Vitamins are natural and indispensable nutrients that are essential for our bodies in small quantities. Vitamins are indispensable for a multitude of biological functions, including growth, development, wound healing, maintaining healthy bones and tissues, supporting the immune system, and more. These vital organic compounds exhibit diverse biochemical functions and are essential for metabolic processes. The discovery of vitamins dates back to 1912 when Casimir Funk, a Polish American biochemist, initiated research on their sources, functions, and deficiency disorders. Because of his groundbreaking work, Funk is widely recognized as the pioneer of vitamins.

Types of vitamins

The classification of vitamins is determined by their solubility, resulting in two distinct groups: fat-soluble vitamins and water-soluble vitamins.

Fat-soluble vitamins

Fat-soluble vitamins, as their name implies, need fat for absorption and are stored in the fat cells. These vitamins include Vitamin A, D, E, and K.

Most of ultrasound machines are designed for imaging with a particular resolution linear and convex transducers. These machines can be used for general purpose for abdominal, obstetrics, gynecological, small parts, urology and cardiac applications. Most of the machines have standard measurement software viz. distance, area, volume, circumference, and obstetrical gestational age measurement for BPD, HC, AC, FL, GS, CRL, AD and FW.

Assessment of probes: One should examine the probe whether it is linear array or curved (convex) array. The probe should also be checked for its frequency. These days multi-frequency probes are supplied with the ultrasound-machine. These multiple frequencies could be 2.0 to 5.0 MHz with centrally fixed frequency of 3.5 MHz. The frequencies could be 4.0 to 7.0 (5.0 MHz setting), 5.0 to 9.0 MHz (7.5 MHz setting). The area and depth covered by various frequencies should be known.

Before setting the ultrasound machine, one should make sure of all the auxiliary devices for the machines. These are Thermal video printer, Ultrasound gel, Biopsy needle, image saving devices (USB device or internal saving mechanism), wire, Uninterrupted Power Supply etc.

Identifying and knowing the main system: One should note down the name and model of ultrasound machine as these are quoted in all the documents published with the ultrascan images generated on that particular machine. It is written on one side of front panel. On the front panel, there are various knobs for proper setting. One should locate the auxiliary power switch of the machine to begin with. Generally, there is power indicator on the machine that shows light on when power button is pushed to on position. The auxiliary power switch may also have indications of its ON and OFF positions, may be marked with 0 and 1 or other symbols. As shown in the figure below, there are various operating buttons on the operating panel. These might vary from Machine to Machine.

Failure to accurately diagnose ovarian function is said to be one of the reason for poor reproductive efficiency in this species. The information regarding ovarian function can be gathered either by observing the reproductive behavior of the animal, status of reproductive hormones or palpation of ovaries per rectum for the presence of cyclic or pathological structures. However, due to poor expression of estrus signs behavioral observations poorly reflect the accurate information about ovarian function in buffaloes. Similarly, palpation per rectum does not provide reliable diagnosis since the CL is frequently embedded deep in the ovarian stroma of buffalo ovary and findings vary due to its subjective nature. Furthermore, measurement of hormones requires sophisticated laboratory procedures/ instruments for making a correct diagnosis. Therefore, real time ultrasonic examination is a better alternative to ascertain reproductive status of buffaloes and for devising suitable strategies to optimize fertility of this species (Sharma, 2003). However, it is important to mention that information gained from ultrasound must be used in conjunction with reproductive history of the animal, palpation of genital tract, and other diagnostic procedures rather than in isolation.

Ultrasonography has an importance place in human practice and veterinary f ield especially in canine practice. Since many years two dimensional ultrasound has been used for scanning purposes both in human and animal practice. But the scenario has changed in human practice with development of three dimensional technology and later four dimensional ultrasonography has developed which shows movements of the objects being scanned especially in the case of fetuses. Nowadays it has been common to use these advanced technology in the human practices. Yet these technologies are in the childhood stage in the veterinary field. High cost of 3D/4D ultrasound equipment, operational difficulties in non sedated animals & lack of specific knowledge about the operation of volume scanning hinder the use of this technology in the veterinary field. Better understanding of the different application of these technologies can be made to use in research areas for the better understanding of the abnormalities, post therapeutics as well in the diagnosis of the diseases

Principle of Three Dimensional and Four Dimensional Ultrasonography

Sound waves of frequency higher than human audible frequency usually ranges from 2-13 MHz are used in ultrasonography. Depending upon on depth of tissue from the scanning surfaces frequency is selected. Three dimensional ultrasonography gives three dimensional images of the volume being scanned .it works on the same principle of 2D ultrasonography but ultrasonographic beams are sent different angles to get three dimensional image of object being scanned. Volume information is obtained either directly using 2D array probe or by reconstructing three dimensional volume from a series of continuous or non- continuous 2D images by using software (Fenster and Downey, 2000). An advancement of 3D technology is 4D ultrasound. Four dimensional ultrasound adds the fourth dimension as time and gives a dynamic real-time 3Dultrasonography of the concerned organ with a continuous volume image acquisitioned rendering process. (Khurana and Dahiya, 2004).

The ultrasonography is based on echo-principal i.e. reflection of sound waves. The ultrasound waves, commonly termed as ultrasound beam is generated from the pizzo-electric crystals, under electric stimulation. The sound beam travels and strike to the organs/tissues on its way and get either reflected or reabsorbed by these structures. The reflected beam is received back by the transducer, which are processed and displayed on the monitor of ultrasound scanner. The display of signal is in form of shades of grey scale i.e. from bright to black picture. If these sound beams strike to organ such as bone, then major part of the beam is reflected and it appears bright (white) on screen. If the sound waves strike to fluid, these get absorbed and are not reflected back, therefore, the image of fluid appears as black on the screen. Other organs appear in between black and bright white. In fact, the reflected sound beam is not proportionate to frequency and velocity of emitted ultrasound beam. The strength of ultrasound beam (grey scale) or brightness of the image depends upon the acoustic impedance of organ or tissue. In simple term acoustic impedance is the capacity of tissue or organ to reflect or absorb these sound beams that again depends upon density or composition of these structures. The reflection of sound beams also depends upon, the angle at which the ultrasound beam strikes the structure on its way. The distance of the organ from the transducer also affects image brightness.

Ultrasonography in goat is becoming popular in the field of reproduction and replacing the other old-age techniques, viz. abdominal ballotment, recto- abdominal probes, X-rays and radioimmuno-assays. The reasons are numerous such as unsatisfactory results, hazardous effects to animal, operator or environment, high cost, long delay in diagnostic results and requirement of sophisticated labs. The ultrasound system has low operating cost and does not need special labs as it can be done at farm itself. Ultrasonography is considered safe to the animal and the operator and provides quick and reliable results. The popular opinion about ultrasonography is that it is noninvasive imaging system and is rapid, safe and practical means of diagnosis of pregnancy and reproductive disorders in sheep and goat. The B-mode real time ultrasonographic system particularly portable system is popular among researcher and practitioners. In goat, B-mode real time ultrasonography was used in 1971, using 5 MHz frequency transducer. A transducer of switchable frequency from 7.5 to 5.0 is more popular in small ruminant research and practice.

Introduction

Acute lung injury (ALI) is the manifestation of an inflammatory response of lung resulting from severe sepsis, bacterial or viral pneumonia, trauma, and burns in animals (Ali et al., 2020).It is distinguwashed by disruption to the alveolar capillary membranes, atelectasis of the pulmonary airspaces, and neutrophil and protein-rich fluid infiltration into the alveolar space (Li et al., 2019). Although advances in treatment, the disability and fatality rates of ALI remain high, that is around 30-40% (Ali et al., 2020). At the moment, new therapeutic alternatives are urgently required to treat ALI. As a result, this is a very active area of research to understand the molecular principles underlying pulmonary inflammation and ALI (Reiss et al., 2012). The use of animal models of inflammatory lung has allowed for the probing of pathways of inflammation responsible for injury. Specially, rodent models may serve a link between in vitro research to large animals and clinical studies by allowing for high quantities of samples under physiological circumstances at low expenses (Domscheit et al., 2020).LPS, found in the outer membrane of gram-negative bacteria, consists of a polar lipid head group (lipid A) and a chain of repeating disaccharides (Gao et al., 2023). LPS induced lung injury model, closely mimic sepsis and inflammatory lung diseases, is a well-establwashed model in this field of research (Reiss et al., 2012). To mimic acute lung injury, LPS can be administered either directly through intratracheal or intranasal route (direct pulmonary insult) or indirectly through intraperitoneally and intravenous routes (indirect extra pulmonary insult) (Perl et al., 2011). This chapter focuses mostly on rodent model of acute lung injury caused by lipopolysaccharide (LPS), both direct and indirect. Additionally, it also includes the characteristics and underlying molecular mechanism of LPS induced lung injury along with applications in drug development.

Introduction

The immense use of chemicals and pharmacological entities has put the public health at brisk of potential health hazards. These substances in modern days are presented in the form of common constituents of food, medicines, beverages and junk foods etc. However, long term consumption of these substances might be responsible for chronic toxicity, but the accidental ingestion of large quantities of these substances may result into adverse acute toxic reactions. Hence an experimenter should have the acumen to develop and perform the acute toxic tests in order to find out the lethal dose 50 i.e. LD50 of the substances.

Acute Toxicity Studies

Acute toxicity studies helps to establish the dose dependent adverse effect including mortalities. The LD50 has been used as a major parameter in screening of pharmacological agents for acute toxic potential. Apart from mortality, other biological effects and the time of onset, duration and degree of recovery on survived animals, are also important in evaluation of acute toxicity. Hence, acute toxicity study solely gives information about LD50, therapeutic index and the degree of safety of a pharmacological agent.

Acute toxicity is defined as adverse effects occur following oral or dermal administration of a single dose of a substance, or multiple doses given within 24 hours, or an inhalation exposure of 4 hours. Oral administration is the most common form of acute systemic toxicity testing.

Insects development

Once an insect hatches from the egg it is usually able to survive on its own, but it is small, wingless, and sexually immature. Its primary role in life is to eat and grow. If it survives, it will periodically outgrow and replace its exoskeleton (a process known as molting). In many species, there are other physical changes that also occur as the insect gets older (growth of wings and development of external genitalia, for example). Collectively, all changes that involve growth, molting, and maturation are known as morphogenesis.

Metamorphosis

• Metamorphosis actually means change in form Metamorphosis is derived from Greek word ‘Meta’ = Change, ‘morph’ = form or structure.

• The changes in the development from egg to adult, collectively called Metamorphosis (life cycle).

• Post-embryonic changes in form are collectively called as metamorphosis.

• Metamorphosis refers to the way that insects develop, grow, and change form. Metamorphosis include three developmental processes namely growth, differentiation and reproduction which takes place in larval, pupal and adult stages, respectively.

• Majority of insects pass through profound metamorphosis. The growth of an immature insect is accompanied by a series of molts or ecdysis, in which the cuticle is shed off and renewed. The number of molts varies in most insects from 4-8. However some undergo as many as 20 or more molts. A few insects like Bristle tail silver fish (apterygota) may continue molting even after reaching the adult stage. But most of the insects neither molt nor increase in size once then adult stage is reached. The intervals between the ecdysis are called Stadia. The term instar is applied to form of an insect during stadium. In numbering the instar, the form assumed by the insect between hatching from the egg and the first post embryonic molt is termed “First Instar”.

5.1 Crop modelling

• Crop model

• “Schematic representation of the system’’i.e. a model as defined by De Wit 1970.

• Such a model can be based on statistics applied to a set of experimental data or on physical laws (Guyot, 1997).e.g. Irrigation is able to satisfy a crop’s water needs provided its management relies on the measurement or estimation of evapotranspiration. The manager can either invest in equipment to monitor changes in water content in the soil or fluxes of water into the plant or the atmosphere, or rely on a ‘‘schematic representation of the system’’, i.e. a model.

• In very simple terms we can define a crop model as “a well-thought out plan of activities to grow the desired crop to get maximum possible yields of high-quality produce with the given resources. It can be a mental or a written exercise (a “schematic representation of the system”) that a farmer or a farm manager prepares for a crop or for an individual crop”.

• In other words, we can also say that a model is “an attempt to describe a certain process or system through the use of a simplified representation, preferably a quantitative mathematical expression, that focuses on a relatively a few key variables that control the process or system”.

• It is clear that in finalizing a crop model we have to assume some mean or standard values (based on the past record, experience, expert judgment, etc.) of the variables which we may not be taking explicitly in our model.

Introduction

• Successful horticultural production is not possible without sufficient moisture regimes and for that, the appropriate techniques should be adopted.

• In the total cultivated land of India, 75 % of land contributed as a rainfed area or dry land area in which 60% with semi-arid and 19.3 % of area with arid zone.

• The irrigation facilities are covered only 38 % of the total cultivated land of the country.

• The moisture is always the limiting factor in arid area.

• So, conservation of soil moisture or water management is the most important task for arid fruit production.

• Out of total cultivated area of Horticultural production, l lakh ha is under arid or semi-arid.

• In arid area, some fruit crops with specials features are adapted for their production. So, in this area, the soil moisture conservation or the water harvesting techniques is the prime important tools for crop production with best management systems.

Increasing pressures exerted on the environment by humans make protection of natural areas crucial for the preservation of biological diversity. Protected areas are one of the most effective tools available for conservation of biodiversity.

The IUCN defines a Protected Area as ‘an area of land and/or sea specially dedicated to the protection and maintenance of biological diversity, and of natural and associated cultured resources, and managed through legal or other effective means.

Since 1986, the IUCN commission on National Parks and Protected Areas (CNPPA) has been promoting the establishment and management of a global representative system of Marine Protected Areas (MPAs) which play a critical role in the conservation of biodiversity. MPAs are growing in importance globally as practical and potentially effective options for the management of fisheries, the protection of biodiversity and the generation of income from eco-tourism. The effective management of MPAs to ensure that they meet their declared objectives poses many challenges.

The IUCN has defined an Marine Protected Area (MPA) as ‘Any area of the intertidal or subtidal terrain, together with its overlying water and associated flora, fauna, historical and cultural features, which has been reserved by law or other effective means to protect part or all the enclosed environment (Kelleher,1999).

INTRODUCTION

The Food and Agriculture Organization (FAO) of the United Nations defines aquaculture as the cultivation of aquatic organisms such as fish, mollusks, crustaceans, and aquatic plants by individuals or corporations. In aquaculture, interventions are made in the rearing process to enhance production. While aquaculture is a practice with origins dating back more than 3000 years, Aquaculture products have only recently been recognized by the United Nations Statistical Commission as distinct global commodities, despite the practice having origins dating back more than 3000 years. According to the FAO's "Global Aquaculture Production" database, the annual global production of aquaculture has increased at an average rate of around 8% over the past three decades (since 1980). Despite a recent slowing in global trends, growth remains higher than any other major animal food production sector. This rapid global increase in production is occurring alongside a slow decline in marine fisheries catches. There is a growing global focus, largely driven by demand from Western countries, on increasing the intensive production of omnivorous and carnivorous species farmed and harvested in maritime and brackish coastal environments, a practice known as mariculture. The farming of these species, such as salmon, groupers, seabasses, and prawns, typically relies heavily on inputs such as wild post-larvae and juveniles, fishmeal, fish oils, as well as significant amounts of water, land, and energy. As a result, mariculture has faced widespread criticism due to its often negative impacts on marine and coastal ecosystem health. Negative associations have been drawn between mariculture and its effects on marine capture fisheries and food security.

INTRODUCTION

The genus Pallisentis was erected by Van Cleave, 1928 with P. umbellatus as its type species. The other species reported under this genus are P. gaoes (Mac Callum, 1918) Van Cleave, 1928; P. cleatus Van Cleave, 1928; P. ophiocephali (Thapar, 1930) Baylis, 1933; P. nagpurensis (Bhalerao, 1931) Baylis, 1933; P. nandai Sarkar, 1953; P. colisai Sarkar, 1956; P. basiri Farooqi, 1958; P. allahabadi Agarwal, 1958; P. buckleyi Tadross, 1966; P. pandei Rai, 1967; P. guntei Sahay et al., 1967; P. garuai n. comb. Davendrosentis garuai Sahay et al., 1971; P. magnum (Saeed & Bilqees, 1971); P. tetradontae Troncy, 1978; P. clupei Gupta and Gupta, 1979; P. cavasi, P. fasciati, P. gomtii Gupta and Verma, 1980; P. indica, P. croftoni Mithal and Lal, 1981; P. kalrai Khan and Bilqees, 1985; P. guptai, P. mehrai Gupta and Fatma, 1985; P. fotedari Gupta and Sinha, 1991 and P. jagani Koul et al., 1991.

MATERIALS AND METHODS

The fish hosts were collected from the study area and brought to the laboratory for parasitic examination. The external body surface as well as the internal body organs were thoroughly examined for the parasites. The collected acanthocephalan parasites were washed and relaxed in normal saline. Then the specimen was fixed and preserved in AFA (alcohol-formalin-acetic acid) solution. To facilitate identification of the worms the parasites were cleared in lactophenol and mounted in glycerine jelly. Diagrams were made with the help of camera lucida and measurements were taken with ocular micrometer. All the measurements are in millimetres. The collected parasites were identified following Yamaguti (1963) and Bhattacharya (2007).

INTRODUCTION

In nature water is considered as the most important natural resource which is utilized in large quantity throughout the world for drinking, washing, bathing, recreation, irrigation, aquaculture and many industries. Water also plays a fundamental role in regulation of water cycle in nature. India is a agriculture based country totally based on different water sources. The quality of water plays a important role to mankind because it is directly linked with human health. Lake water’s are mainly used for farming and domestic activities like cloth washing. Increasing human activities continuously in lake basins is posing a great threat to all forms of life in aquatic environment. Most of the people who live in villages use irrigation facilities for farms and use excessive use fertilizers and pesticides for agriculture purposes and subsequently and degrade the aquatic ecosystems of lakes. We need to conserve water for future generations and safeguard our natural resources for sustainable development and biodiversity conservation.

Many researchers investigated the freshwater lakes and ponds in the Indian subcontinent viz. Bagde and Verma (1985), Desai (2014), Patel and Patel (2012), Nirbhavanae and Khobragade (2017), Mehta et al (2016), Gupta (2018), Bastola (2013), Raj and Sevankodiyane (2018), Rana Phul Kunwar Singh (2016), Shyamala and Hemvathy (2018), Meshram et al (2014), Fule and Nimgare (2018), Chaudhary and Sitre (2020), Pimpalshende and Sitre (2021).

INTRODUCTION

India is rich in biodiversity and possesses about 108276 species of insects. Indian subcontinent is one of the mega biodiversity countries of the world occupying ninth position in terms of freshwater mega biodiversity (Mittermeier et al., 1997) comprising 30 order (Dijkstra et al. 2013). Inland wetlands of India serve as the habitat for more than 500 species of aquatic insects which are represented predominantly by Ephemeroptera (mayflies), Odonata (dragonflies) and Trichoptera (caddisflies), (Subramanian and Sivaramakrishnan, 2007). Aquatic insects play important ecological roles in keeping freshwater ecosystems functioning properly (Choudhary and Janak, 2015). The aquatic insects are important part of aquatic ecosystem which play vital role in ecosystem functioning and are known to be representative of structure and function of freshwater ecosystem due to their high abundance, high birthrate with short generation time, large and rapid colonization (Choudhary and Ahi 2015). Aquatic insect function on multiple trophic level such as shredders, collectors, scraper and predators on animal debris, wood and leaf litter reaching the wetland from the surrounding landscape. Nutrients processed by aquatic insects are further degraded into absorbable form by fungal and bacterial action. Plants in the riparian zone absorb this nutrient soup transported through the wetlands. In addition to this significant ecosystem function, aquatic insects are also a primary source of food for fishes and amphibians (Tachet et al., 2003 ; Kumar, 2014).They interact in nutrient cycling within lentic and lotic systems and are regarded as an effective bioindicator (Lundquist and Zhu 2018) and serve as an important component of natural foodweb in aquatic ecosystem. However, the aquatic insect fauna of this part in India is inadequately documented. Therefore, present study carried out to delineate the baseline status of aquatic insects of Anandwan lakes at Warora Dist: Chandrapur.

Order: Siphonptera (Fleas)
Class: Insecta
Subclass: Pterygota 
Division: Endopterygota
Order: Siphonaptera
Siphonaptera derived from two greek words viz. siphon (sucking) and apteron (wingless)
General characters of fleas
1. Body is laterally compressed.
2. Tegument is highly scleritized. Outer chitinous layer is very thick and it is dark brown in colour.
3. A pair of simple eye or ocelli are present on head. Compound eyes absent.

Acaricides: Acaricides are agents that kill members of class Arachnida like ticks and mites.

Acaricides are used both in medicine and agriculture, although the desired selective toxicity differs between the two fields.

What is acaricide resistance?

The ability of a parasite strain to survive and/or to multiply despite the administration and absorption of a drug given in doses equal to or higher than those usually recommended but within the limits of tolerance of the subject.

How does resistance develop?

• Animals are treated with the chemical (Acaricide), exposing nearly all of the tick population to the toxin.

• Susceptible individuals are killed, and those rare individuals that have some genetic resistance, survive.

• They reproduce, and pass along those “resistance genes” to the general tick population.

1. Collection of arthropods

a. Collection of arthropods from the host’s body: The collection of arthropods from the host’s body involves various techniques tailored to different species and circumstances. For instance, the extraction of small ectoparasites such as lice, fleas, and wingless keds typically employs usage of forceps with pointed tips for precision. In poultry, a method of brushing against the grain of feathers enables dislodgement of ectoparasites, which are then collected on a black surface for examination. A highly effective strategy for harvesting ectoparasites from small or deceased hosts, such as poultry, involves sealing the carcasses within a polyethylene bag. This confinement prompts ectoparasites to detach from the host and accumulate within the bag, facilitating subsequent collection using a brush. The retrieval of ticks to prevent the rupture of their mouthparts is achieved by straightening the tick forward and pulling backwards before extraction. These ticks are further placed in a tube sealed with a ventilated stopper or a gauze covered with cotton wool plug. The tubes ought to include paper for the ticks to cling onto, along with a labelled piece of paper. These tubes are stored in a cool and humid container for transportation to the laboratory. This container should be well-insulated and equipped with ice blocks or freezer packs as well as damp pads to sustain elevated humidity levels. It’s crucial to ensure that the ticks do not directly touch the ice. A sizable thermos flask can be utilized for this purpose. Mange mites, on the other hand, are obtained by conducting deep skin scrapings from the periphery of affected lesions, enabling the collection of specimens for analysis.

Introduction

The most important function in the socioeconomic domain of India is played by agriculture, which is the foundation of the nation's economy. India's agriculture industry is vast, varied, and involves a lot of different players. With the use of Green Revolution technologies, it has become one of the most amazing success stories of the post-independence era. The Green Revolution enhanced rural welfare and allowed for food self-sufficiency, both of which boosted the Indian economy. In the framework of the Green Revolution, the National Agricultural Research System (NARS) played a crucial role. India boasts one of the world's biggest and most intricate agricultural research programs in terms of structure. The Imperial (now Indian) Council of Agricultural Research (ICAR) was established in 1929 on the advice of a Royal Commission on Agriculture, marking the culmination of a process that began in the 19th century and led to the creation of the current Indian agricultural research system [1].

The functioning of the agricultural sector, market dynamics, and policy consequences are all influenced by a complex web of interrelated components that make up agricultural economics dynamics. These dynamics shape the production, supply, demand, and competitiveness of agricultural products and involve the interactions between farmers, markets, technology, and government regulations [2],[5].

Introduction

The world of finance revolves around the management, generation, and analysis of money and investment opportunities. Financial professionals orchestrate a complex dance involving credit, borrowing, and various investment instruments like securities and stocks. This intricate choreography aims to fund present endeavours using a strategic allocation of anticipated future income. Since finance deals with the flow of money across time, grasping the time value of money is essential. Understanding interest rates and how they impact investments becomes paramount for making sound financial decisions that consider both present needs and future goals. This intricate web of concepts is what finance professionals navigate to ensure the optimal use of financial resources. Finance can be broadly divided into three types:

1. Public finance

2. Corporate finance

3. Personal finance

1. Public Finance: The government steps in to prevent the economy from going off track by managing how resources are distributed, income is shared fairly, and the economy remains stable. Taxes are the main way the government gets the money to fund these programs.

Introduction

The economic cycle, also known as the business cycle, refers to the fluctuating state of an economy characterized by periods of economic expansion and contraction. It involves the cyclical pattern of growth and decline in economic activity, impacting various indicators like gross domestic product (GDP), employment rates, interest rates, and consumer spending2. The economic cycle is a fundamental concept in economics that helps determine the overall state of an economy as it moves through four distinct stages: expansion, peak, contraction, and trough. Understanding the economic cycle is crucial for investors, businesses, and policymakers as it influences investment decisions, business strategies, and economic policies based on the prevailing stage of the cycle.

1. Expansion

The upswing of the business cycle towards a peak is called an economic expansion. An economic expansion is associated with: Firstly, an increase in production and output is a prominent feature of economic expansion. This uptick in production signifies growing economic activity, as businesses ramp up their operations to meet rising demand.

Introduction

Over the course of past decades India is one of the fastest growing economies of the world with a robust growth and transformations in its economy. In this journey subsidies played a crucial role in influencing market behaviour, support industries, address market failures, and achieve social and economic objectives of the country. Now what actually the economic subsidies are.

Subsidies are financial incentives provided by governments to individuals, businesses, or industries in the form of cash, grants, or tax breaks to influence economic behaviour and outcomes. They aim to correct market failures, promote specific industries, encourage production, and address economic challenges. Subsidies can be direct (cash payments) or indirect (tax breaks), benefiting recipients by reducing burdens or promoting certain actions. They are in the public interest, supporting social goods or economic policies.

The importance of subsidies lies in their ability to offset market failures and externalities, leading to greater economic efficiency. By providing financial aid, subsidies can stimulate production, increase supply, lower prices for consumers, and drive economic growth in targeted sectors. They play a crucial role in stabilizing economies, responding to economic shocks, and promoting long-term economic stability. Subsidies can also help governments address poverty, encourage investment in research and development, and support essential industries like agriculture, energy, and healthcare.

India has a unique social and cultural setting with diverse people, culture and religions. It is one of the oldest and greatest civilizations of the world. Geographically India extends snow clad Himalayan mountains in the north to the tropical rain forests of the south. It is the 7th largest country in the world and well recognised in Asia by its mountains, seas, deserts, and vast stretch of fertile plain land, which give the country a distinct geographical identity.

India has a very rich, diverse religious and cultural heritage. The four main religions of India are - Hinduism, Islam, Christianity and Sikhism. Hinduism is the largest religion in India, with approximately 80% of the population is Hindu while Islam (Muslim) is the second-largest religion having 14% (approx.) share.

Religion in India is characterised by the diversity of religious beliefs and practices. In Indian history, religion has been an important part of the country's culture. Indian subcontinent is the birthplace of four of the world's major religions, namely, Buddhism, Hinduism, Jainism and Sikhism, which are collectively known as native Indian religions and represent 83% of the total population of India approximately.

Hinduism

Hinduism is the main Indian religion. It is considered as a religious and universal order, a way of life which followed by the people who believed in Hinduism. The word ‘Hindu’ refers group of particular people who believes and follow Hinduism, the oldest religion in the world. It has also been described as ‘Sanatana dharma’.

Pineapple [Ananas comosus (L.) Merr.] is one of the most importanttropical fruit belonging to the family Bromeliaceae and is native to Brazil. It is cultivated in High rainfall and humid coastal regions of peninsular India and Hilly areas of North eastern states. In India, pineapple is commercially cultivated in the states like West Bengal, Assam, Karnataka, Bihar, Tripura, Kerala, Nagaland, Goa, Arunachal Pradesh, Manipur and Meghalaya on large scale whereas on small scale in the states like Gujarat, Andhra Pradesh, Maharashtra, Tamil Nadu, Orissa and Uttar Pradesh etc. It is popularly known as ‘Ananas’ in India and has lot of health benefits due to the presence of a proteolytic enzyme called bromelin, which is used to tenderize meat (Coppensd’ Eeckenbrugge, 2011). It acts as antiamoebic, arbortifacient and also used in correction of stomach disorders (Leal and Coppensd’ Eeckenbrugge, 1996). It is consumed both fresh and processed but mostly as fresh (70%) (Loeillet, 1997). Portuguese are the first people to introduce pineapple to India. The greenhouse cultivation of pineapple was first started in 1500s by Europeans, which led to the varieties like Smooth Cayenne and Queen and transported to all tropical and subtropical regions of the world (Collins, 1951). Due to its ease in transport of its propagules and high tolerance to drought, pineapple cultivation has spread worldwide. But due to its short shelf life, in temperate regions it is consumed more as processed food. The first commercial product made out of pineapple is jams and sweets (Thevet, 1957).

Pineapple is a monocarpic perennial herb. Each stem flowers only once and dies after fruiting, similar to bananas, before a side shoot takes over. After the pineapple plant reaches a certain size, natural inflorescence initiation (flowering) occurs in response to decreasing day length and temperature (Bartholomew, 2003). Flowering of Smooth Cayenne pineapples grown under an 8-hour day length was encouraged by a night temperature of 20°C, whereas f lowering was delayed by temperatures of 15 and 20°C, and totally inhibited by 30°C.Flower initiation usually starts in November and lasts through the winter. Flowering in nature is highly irregular, and some plants can fail to produce fruit. Thus, growth regulators such as ethylene (25-50 ppm) and NAA (25 ppm) are used in industrial practise to force plants to bloom. The numerous radish-purple sessile flowers are spirally arranged on the axis, each with a pointed bract. To explain the emergence of developing inflorescence, this stage is also known as the red bud stage. The spike-like inflorescence contains 100 to 200 florets. The petals are white at the base and bluish-purple above the calyx at anthesis. They are ligulae form in shape, about 1.6 cm long and 5 mm wide, and are about 1.6 cm long and 5 mm wide (Collins, 1960).The outer f lower petals are very close together, allowing only small insects to reach the f lower; they have 14 to 20 locules, resulting in a capacity of 6000 to 7000 seeds per 150 floret fruit. After pollination, fruits with 2000 to 3000 seeds are not uncommon (Collins, 1960).

Successful cultivation of a crop is governed by various factors including the edaphic and climatic conditions of the region, owing to which the crop responds in terms of quality and productivity. Pineapple (Ananas comosus L. Merr.) is one of the major crops cultivated across the tropical and sub tropical countries worldwide. Adaptability of the crop under different climatic conditions, topography, temperature, photo-periodicity, rainfall and edaphic conditions will be discussed in this chapter.

Climatic zone: Pineapple is a tropical fruit crop though it can thrive well in the sub-tropical condition as well.Pineapple grows well inwarm and humid climate ranging from mild coastal climate up to an altitude of about 1000 meters, provided the area is free from frost (Singh, 2014).Pineapple is commercially grown over a wide range of latitudes from 30° N (Assam, India) in the northern hemisphere to 33°S in the Southern hemisphere (South Africa) (Malezieux et al., 2003; Purseglove, 1972).

Topography: Pineapples are easy to cultivatesince they thrive naturally in a variety of places with different elevation, especially in tropical countries (Kaudo, 2014). Though the plant survives at different altitude, the fruit quality and size may alter at different elevation. When pineapple is grown at altitudes greater than 1000 m, smaller fruits with elevated tartness with less attractive pulp colour (Purseglove, 1968).Moreover, fruit flavour can also vary, wherein the fruit tends to bemore acidic and sour when cultivated above 1800 meters, but will be sweet and tasty under 1350 meters (Kaudo, 2014).

Temperature: On an average, the temperatures required for growing pineapples range within 18-30°C (Kaudo, 2014) andmore specifically at 23 - 24 ° C (Neild and Boshell, 1976; Py et al., 1987). The optimum day and night temperatures for pineapple were reported to be about 30°C and 20 °C, respectively (Neild and Boshell, 1976). Bartholomew and Kadzimin (1977), however have reported that leaves and roots grow best at 32°C and 29°C respectively and the growth practically ceases below 20°C and above 36°C. Py and Tisuucau (1965) have observed 25°C to be optimal for fruit ripening.

Horticultural sector, which is contributing significantly for food and nutrition security, employment opportunity and poverty alleviation, got a real boost only in the early 1990s which coincided with liberalization of Indian economy. National area and production of horticultural crops are showing increasing trend over the years and production of horticultural crops has outpaced the production of food grain since 2012-13(Press Information Bureau, Govt. of India, Ministry of Finance, 2016). According to Economic Survey 2015-16, the share of horticultural output in agriculture is more than 33% over last decade and the sector contributes 30.4% to GDP of Agricultural sector. Horticulture sector accounts for 37% of total export of agricultural commodities at present and the exports have recorded sustained rising trend (Jha et al., 2019). The export status of APEDA (Agricultural and Processed Food Products Export Development Authority) products in the recent years shows 28.18% growth in value of horticultural products over the previous year. The investment in horticultural sector with focused attention has resulted in spectacular change in the horticultural scenario and production and productivity of horticultural crops.

1. ABSORPTION

Deals with the translocation of the xenobiotics. Passage of toxicant from site of adminstration to blood stream or systemic circulation.

Translocation may occur by following processes

a) Simple /Passive diffusion - most common route by which xenobiotic pass through the membranes:

? Down hill transport, No energy involvement

? It is not substrate specific.

? Small molecular weight (600) hydrophilic toxicants cross membranes by simple diffusion through aqueous pores.

? High molecular wt. hydrophobic molecules diffuse across the lipid domain of membranes.

? Follows Ficks Law of diffusion

? The rate of diffusion depends on their lipid: water partition coefficient value, Conc. Gradient, degree of ionization.

? Higher lipid: water partition coefficient and lower degree of ionization favours absorption of toxicants by this process.

Key Points

• Patient assessment is only half of the disease diagnosis process.

• Using animal physiological values as a reference point can help measure a patient's health.

• Laboratory results support the patient's evaluation results.

• Patient evaluation findings suggest bodily system involvement.

Introduction

The patient assessment includes the owner's complaint, history, physical examination, and clinical examination of the animals (Fig. 1.1). Proper animal assessment is only part of the illness diagnosis process. Sometimes, evaluation results can be indicative of a medical condition. As a result, at the initial stage, assessing the animal aids in making a confirmation diagnosis. Given its significance, an assessment of the animal must be performed or obtained before concluding the laboratory finding, if available. Assessment can be conducted in several steps, each of which is detailed in the next paragraph. The physiological vital parameters of animals are crucial reference points to conclude the evaluation findings.

Key Points

• Animal bites containing poisons, venoms, or diseases cause significant morbidity and mortality.

• Unattended animals are vulnerable to various types of bites, including snakes, spiders, scorpions, bee stings, lizards, and dogs.

• Snake bites can be life-threatening for both humans and animals and require emergency treatment.

• Dog bites can cause rabies in both people and animals.

Introduction

If left alone, curious animals are likely to be bitten by a variety of insects. Bite severity varies by animal species. Typically, stings and bites are painful and dangerous. It can be life-threatening in certain situations.