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Today around 25% of rural population in India are between the age group of 15 and 29 (2001 Census).  The Tenth Five-Year Plan (2002-2007) has estimated that India's labour force will increase faster than the ability of the economy to create new working opportunities. The country is adding 2 million young people to the ranks of the unemployed every year. The Plan document estimates that open unemployment could be as high as 5% at the end of the Tenth Plan period, from 2.8% at present.  This is likely to entail tremendous costs, including social unrest and dislocation resulting with large number of youth are unemployed or underemployed.
 
In India and in many developing countries the majority of the work force is employed in agriculture. However, stagnating agricultural productivity and rural environmental degradation have made agriculture a last option.  As the environment on which their livelihoods depend becomes more and more degraded, rural youth face diminished prospects of employment.  Not only do these youth lack income, they lack a means of gaining respect and a sense of belonging in their communities.  Unable to find decent employment, youth often look for employment in the urban informal sector, with poor working conditions and pay.  But without training in skills suited to the rural labour market, these youth have few opportunities in rural areas.  Inadequately trained to compete successfully in rural labour markets, they often suffer worse levels of poverty and marginalization in rural areas.

India is on the move, but in part.  
For, the farm sector is lagging behind.
It may soon become a drag on the economy.
Hence, farm development requires renewed efforts,
Farm youth must get critical attention.
Matter brooks no delay.

Farming is still the only source of human subsistence. No alternative is in sight. This ancient family occupation has survived over the ages through a process of transfer from father to son, generation to generation. But, in recent times, with an unprecedented externalization of farming, and under the pervasive influence of Liberalisation, Privatisation and Globalisation, this process of transfer of occupational knowledge within the community is seriously dislocated. Also, the earlier institutional arrangements for formal education in farming like district agricultural schools, gramsevaks training units and farmers training centres have now disappeared. In the meantime, even as more rural youth are becoming literate, formal education system is seen to have shed all aspects of agricultural education. Thus, the rural drop-outs are missing both formal and informal orientation in farming since a comprehension of ‘modern’ farming is beyond the middle level farmers. As such, farm youth today are neither here nor there, with regard to farming.

Abstract

Extension and Advisory Services (EAS) are delivered by millions of extension professionals representing the public, private and civil society located across the globe and have been helping in addressing farmers’ needs over the years. However, public sector has been a major agency dealing with EAS in India and is facing several challenges and constraints to fulfill the demands of farmers on timely basis. The challenge today is to change the organizational culture to incorporate innovation as a core value and to institutionalize the emerging paradigms. Further, different strategies and measures need to be taken to ensure timely and quality EAS by reorienting extension priorities. This paper has highlighted on strengthening extension and feedback mechanism, improving research- extension linkages, capacity building, public-private partnership (PPP), developing infrastructure, mass media support and use of Information and Communication Technologies (ICTs) etc. to improve the efficiency as the time demands. The paper concludes that reorienting extension priorities is very essential with a vast network of various stakeholders by adapting effective approaches like utilization of social media, human resource development, PPP, farmer groups etc. during and post pandemic scenario. Further, empirical efforts are also needed to develop reliable, location-specific, participatory, gender-sensitive and inexpensive extension methodologies and materials to meet the emerging demands. Further, developing countries like India have to invest in terms of various resources like financial, human resource etc. for promoting higher productivity and sustainability through EAS.

Farmer Producer Organizations represent a transformative institutional innovation in agricultural development that addresses the fundamental challenges of small-scale farming through collective action and cooperative enterprise development. These organizations emerge from the recognition that individual small farmers face insurmountable obstacles in accessing markets, obtaining fair prices, securing quality inputs, and achieving economies of scale necessary for sustainable agricultural livelihoods in increasingly competitive and globalized food systems. The concept of FPOs builds on the rich tradition of agricultural cooperation while adapting to contemporary market realities and policy frameworks that require formal institutional structures for accessing services, credit, and government support programs. FPOs represent a hybrid institutional form that combines the democratic governance and member ownership characteristics of traditional cooperatives with the business orientation and professional management capabilities of modern enterprises, creating organizations that can operate effectively in comp

Introduction
Spices have been considered significant in the culinary art from time immemorial. India, “The Homeland of Spices” is one among the largest producers, consumers and exporters of spices as well as spice products. Spices comprise different plant components such as floral parts or fruits or berries or seeds or rhizomes or roots or leaves or kernel or aril or bark or bulbs. The recorded history of the use of spices dates back to 5000 years or so from the Egyptian or at the most from the Indus valley civilization. Spices are used for flavouring, seasoning and imparting aroma in various cuisines. Besides these inherent qualities, some of them are known to be fungistatic, antimicrobial or antibiotic. They are also processed into numerous ‘value added’ products such as oleoresins, essential oils, curry powder etc. Black pepper, cardamom and turmeric are the major spice crops and its production and marketing are the main economy stays of lakhs of people of the country. Billions of rupees are involved in domestic trading and foreign export. Therefore, it is highly imperative for farmers to adopt the advanced practices to sustain spices productivity. Even, additional income could be generated from the intercropping of spices from the interspaces in mango orchards (Shukla and Adak, 2020). Thus the present chapter included all aspects of production to protection and value addition for  the benefits of growers.

Introduction

Farming system conceptually is an integrated set of activities that farmers perform in their farm with available resources and circumstances to maximise productivity and net farm income on a sustainable basis. The system approach suggest a judicious mix of enterprises and activities based on the cardinal principle of minimising the competition and maximising complimentarity taking into account the components of soil, water, crops, livestock, labour, capital, energy and other resources. With all the resources, enterprises and external forces interacting in the system, the farmer operate at the centre of the farm household to take decisions observing variability, profitability, stability and sustainability. It is not the technology alone, but a set of social, political, institutional and economical forces influence the decision making process in a farming system leading to a model or acceptable combination of enterprises for overall development of the farm household.

Though science and technology have played a vital role in finding out innovations in the farming system approach through addition of new enterprise leading to profitability; new technologies ensuring stability and sustainability, the basic philosophy of the approach based on set principles is being pursued since initiation of agriculture in the civilisation.

In general farming system approach is more of a thought, a movement, a philosophy than simply choosing enterprise or combination of so to improve upon the standard of living. The interaction of bio-physical and socio-economic values plays an important role in up-scaling farming system approach and the adoption behaviour largely depend on the human values rather than material gain.

With the changing scenario, several factors like increasing instability in production, market price, high cost of production inputs, labour and negative impact of climate change, degradation of natural resources directly or indirectly warrants the use of sustainable rather regenerating technologies, compatible and noncompetitive farm enterprises to bring in sustainability and enhancement in production and income. For more than a century, programmes and activities have been designed to address the vulnerability of rural poor and are often targeted towards improving agricultural practices as means of increasing productivity, efficiency and ultimately income. In majority of the TOT cases technology has taken the centre stage even though the basic fundamental question like ‘whether the farmer adopt the offered technology and, if they do, whether they do it in ideal combination and for a considerable length of time needed to produce designed result remains unanswered. More importantly, in general the technology in isolation can not be recommended to all categories of farmers operating under varying situations. On the other hand acceptance and continuity of the technology is a dynamic process and varies both with the quality of the technology being promoted and the adoption behaviour of the individual farmer.

As the world grapples with the complexities of climate change, one group of people has been on the front lines, witnessing firsthand the devastating impacts of rising temperatures, altered precipitation patterns, and increased extreme weather events: farmers. For generations, farmers have been the stewards of the land, relying on their intimate knowledge of the natural world to coax crops from the earth and raise livestock. However, the changing climate is redefining the rules of their trade, forcing them to adapt to new and unpredictable conditions. The relationship between farmers and the climate is deeply intertwined. On one hand, farmers have always been attuned to the subtleties of the climate, carefully observing weather patterns, soil moisture levels, and pest populations to make informed decisions about planting, harvesting, and crop management. This traditional knowledge, passed down through generations, has allowed farmers to develop a keen sense of the local climate and its rhythms. On the other hand, farmers are also significant contributors to greenhouse gas emissions, with agricultural practices such as tillage, fertilization, and livestock production releasing carbon dioxide, methane, and nitrous oxide into the atmosphere. As the climate continues to change, farmers are finding themselves at the forefront of climate awareness. Rising temperatures are altering growing seasons, shifting the distribution of pests and diseases, and changing the patterns of rainfall and drought. This, in turn, affects crop yields, quality, and profitability, making it increasingly challenging for farmers to maintain their livelihoods. The consequences of climate change are far-reaching, from the immediate impacts on farm productivity to the long-term implications for food security, rural development, and ecosystem health. In this context, farmers are not just passive victims of climate change; they are also key players in the global effort to mitigate its effects. By adopting climateresilient agricultural practices, such as agroforestry, conservation tillage, and integrated pest management, farmers can reduce their carbon footprint, improve soil health, and promote biodiversity. Moreover, farmers can serve as powerful advocates for climate policy, using their unique perspective to inform decisionmaking at the local, national, and international levels. This chapter explores the complex relationships between farmers, climate awareness, and climate change. By examining the ways in which farmers are experiencing and responding to climate change, we can gain a deeper understanding of the challenges and opportunities that lie ahead. From the nuances of climatesmart agriculture to the role of farmers in shaping climate policy, this chapter

Social research concentrates more on socio economic variables to find out effects of the events that take place in social system. The magnitude of variation in the variables assumes to bring variation on the effect. The variables relating to farmers of small farms are many and they are specific to farming system of the coastal ecosystem. Such variables were grouped under personal, social, economic, communication behaviour, marketing behaviour, farming behaviour and training. The sub variables under each heading were given in the following table.

Farmers' rights and advocacy are essential for the sustainability and advancement of agricultural communities worldwide. These rights encompass access to land, seeds, resources, and fair market conditions, along with the recognition of farmers' contributions to food security and biodiversity. Advocacy for farmers' rights involves mobilizing stakeholders, influencing policy, and fostering grassroots movements to ensure equitable treatment and support for farmers. This article delves into the historical context, key components, challenges, and effective strategies for advocating farmers' rights, underscoring the importance of a collective approach to empower the agricultural sector

Introduction

Farmers are the backbone of global food systems, playing a crucial role in feeding populations and maintaining biodiversity. However, they often face numerous challenges, including land tenure insecurity, lack of access to quality seeds, limited financial resources, and unfavorable market conditions. Recognizing and advocating for farmers' rights is vital to address these issues and support sustainable agricultural practices. This article explores the historical development of farmers' rights, their key components, the challenges farmers face, and effective strategies for advocacy, emphasizing the importance of collective action to empower the agricultural sector.

1. Introduction

Over the years, Agriculture Extension has been at the fore front within the delivery of adequate data to the farming community not just for increasing productivity however additionally to reinforce their standard of living. Keeping in-sight of the demand for agricultural growth, evolutionary modifications had been made in transfer of technology to succeed in the farming community effectively. The Information and Communication Technology (ICT) is one among the required counterparts which made the agriculture extension more realistic and quite interesting.

Developing nations like India are though capable to get self-sufficiency in food production after green revolution however they’re in risk to keep this self sufficiency as they are possibly to face food shortage in near future due to rapid population growth. Thus, the demand of continuous increasing population is often fulfilled with the assistance of ICT as a tool of revamping extension network of the country. ICT is an emerging tool for development of farming communities.

Information and communication technologies have unique feature that provide opportunities to harness them in ways which are extraordinary from traditional media. ICT provides two-way communication between rural communities and development organizations. ICTs also improves the capacity to look for information and increase the number of information available, provide quality information, reduces uncertainty and enhance market participation.

Agricultural practices have been undergoing changes over a period of time throughout the world. The intensive agricultural practices followed during the past four decades in our country resulted in food security and self-sufficiency. This was achieved through development of input responsive varieties coupled with use of chemical fertilizers and plant protection chemicals. Agriculture is an important sector for economic development in India. Crop production is an important income source for millions of farmers in some cases with few cropping alternatives due to ecological constraints. All over the world majority offarmers rely on pesticides and fertilizers to increase yields. Pesticide use in most Indian agriculture is an essential part of production technology. Therefore farmers in developing countries are perceived as overusing pesticides, both in quantity and quality, with mixtures of chemicals, known locally as cocktails, being the favoured form of application (Crissman et al., 1994).

The world’s population is projected to reach 9.7 billion by 2050, putting unprecedented pressure on the agricultural sector to produce more food while minimizing its environmental footprint. However, the increasing frequency and severity of climate-related disasters, such as droughts, floods, and heat waves, pose significant challenges to agricultural productivity and food security. To address these challenges, farmers, policymakers, and agricultural experts must adopt innovative approaches that integrate climate science, agricultural meteorology, and cutting-edge technologies to generate actionable farming intelligence. Climate science and agricultural meteorology provide critical insights into the complex relationships between weather patterns, climate variability, and agricultural productivity. By incorporating these disciplines into farming practices, farmers can enhance crop yields, reduce climate-related risks, promote sustainable agriculture, and improve decision-making. Understanding weather patterns and climate trends enables farmers to optimize planting dates, irrigation schedules, and harvest times, leading to improved crop yields and reduced losses. Access to accurate and reliable climate information helps farmers anticipate and prepare for climaterelated disasters, minimizing damage to crops and livestock. Climate science and agricultural meteorology also inform farmers about the most effective strategies for reducing greenhouse gas emissions, conserving water, and maintaining soil health. The integration of climate science, agricultural meteorology, and cutting-edge technologies is revolutionizing the agricultural sector. Artificial intelligence (AI) algorithms analyze large datasets, including climate information, soil moisture levels, and crop health, to provide insights on optimal farming practices. Internet of Things (IoT) sensors and connected devices monitor weather patterns, soil conditions, and crop health in real-time, enabling farmers to respond promptly to changing conditions. Geographic Information Systems (GIS) mapping and spatial analysis help farmers understand the spatial distribution of climate-related risks and opportunities, informing decision-making at the farm level (Fig. 3.1).

The prosperity of any country depends upon the prosperity of its people. Food, clothing, shelter, health, education, security, roads, electricity and drinking water are the today’s basic needs. Farmers produce food. About 70 % of Indian population is engaged in farming. Therefore, prosperity of India would depend upon the prosperity of its farmers. This in turn depends upon the adoption of improved technology and judicious allocation of resources (land, labour, capital, machinery etc). Human race depends more on farm products for their existence than anything else since food and clothing – the prime necessaries are products of farming. Even for industrial prosperity, farming forms the basic raw material. To sustain and satisfy as many as his needs, the farmers include crop production, livestock, poultry, fisheries, beekeeping etc. in their farms. Earlier subsistence was the important objective of farming. Farmers took many activities such as planting of fruit trees in their farms or on the common lands just for the welfare of mankind without expecting anything in return. The kind of farming where subsistence and welfare of mankind were the main objectives is designated as mixed farming. Farming today is also a mix of enterprises. However, higher profitability without altering ecological balance is important in farming. A set of agricultural activities organized while preserving land productivity, environmental quality and maintaining desirable level of biological diversity and ecological stability is designated as “Farming System”. Here the emphasis is mainly on a system rather than on gross output.

In other words “farming system” is a resource management strategy to achieve economic and sustain agricultural production to meet diverse requirement of the farm household while preserving the resource base and maintaining high environmental quality. The farming system in its real sense will help to lift the economy of agriculture and standard of living of the farmers.

Introduction

Nature is dynamic; it causes inheritable changes in all living organisms. For thousands of years, farmer around the world have been selecting and conserving varieties of different crop plants that they cultivated. This process has generated a rich wealth of varieties in each crop plant, seen to be most abundant in countries near the equator and India is no exception. In the world there are Mega centers of biodiversity of which aware present in India, this is because in India, the different soils and agro-climatic situations are present, so Indian farmers grow a large number of crops. Generation of Indian farmers, with their continued selection and conservation, has created a rich wealth of varieties in many crops. Therefore India is the original home of many crops such as rice, little and kodo millets, red gram, moth bean, jute, pepper, cardamom, many vegetables and fruit species. These plants were identified from the wild, selected and cultivated by Indian farmers over hundreds of years. The present wealth of varieties in India includes both crops that originated in the country and those that were introduced from the other countries during the distant and recent paste are soybean, sunflower, oil palm and kiwi fruit.

The Indian economy is predominantly rural and agriculture oriented. In agriculture, 85% of the holdings are less than two hectares and the declining trend in the average size of the farmer holdings, poses a serious problem. Majority of them are dry lands, which depend on erratic monsoon rains. The rest of the area is cultivated with supplemental irrigation. The farmers concentrate mainly on crop production, which is invariably subjected to a high degree of uncertainly income and employment.

In India the cultivable land is 143.8 million hectares and there is very little possibility of extending it further. Therefore, to meet the requirement of food grains for increasing population, the only option open is through time and effective space utilization in agriculture. The time concept relates to increasing the intensity of cropping under assured irrigated conditions, whereas space utilization pertains to building up of vertical dimension through multi-tier cropping and farming system approach. Thus by making use of these time and space concept either in irrigated or in rained areas, the productivity per unit area per unit time can be substantially enhanced. Therefore the only way to increase an agricultural production in the small/marginal units of farming is to increase the productivity per unit time and area. This may be achieved of quicker maturing varieties with equal yields or by improving techniques of culture, fertilizer use, weed and pest control.

A ‘system’ is a set of interrelated, interacting and interdependent elements operating together for a common purpose and capable of reacting as a whole to external forces. It is unaffected directly by its own output and it has a specified external boundary based on the inclusion of all significant feedbacks. A ‘farm’ is a system because several activities are closely related to each other by the common use of the farm labour, land and capital, by risk distribution and by the joint use of the farmer’s management capacity.

Farming System Approach (FSA)

Farming systems refers deliberate raising of crops,forest and fruit trees, animals including fisheries, piggery and duck farming, sericulture, mushroom, on a given unit of land to increase the productivity and profitability, to upgrade natural resource base and to achieve overall improvement in the environment

The philosophy behind shifting from cropping system to the farming system mode involves

1. In situ recycling of organic residues including farm wastes generated at the farm to reduce the dependency on chemicals
2. Decrease in cost of cultivation through enhance input use efficiency,
3. Effective use of bye-products/wastes of one component for the benefit of other component/components
4. Upgrading of soil and water quality and bio-diversity,

Introduction

Global warming directly ref lects on rising sea levels due to melting of ice caps and natural expansion of sea water as it becomes warmer. Consequently, areas adjoining the coast and wetlands could be frequently flooded and the distribution pattern of monsoon rains may alter, through more intense downpours, storms and hurricanes. The meteorological data available at the Annamalai University, for the tail end of the Cauvery river delta region of Tamil Nadu State, India, shows that the average annual rainfall during the last 10 years segment has increased by 233 mm compared to the average of the previous 10 years segment (1588 mm and 1355 mm, respectively). In contrast, annual evaporation has reduced by 453 mm (2153 mm and 1700mm, respectively) (Kathiresan, 2011). The year 1990 was the hottest year in the last century with all other five of the warmest years in the century falling within the last 22 years. This trend indicates that f lash floods in wetlands and droughts in uplands have become more frequent. Such a changing climate warrants reinforcement of climate resilience in farming to protect the livelihoods of farmers and the biodiversity. The sub-project of the National Agricultural Innovation Project implemented by Annamalai University in Tamilnadu, sponsored by ICAR has identified three types of clusters in each of the four disadvantaged districts, based on the prevailing agro-ecology viz. wetland clusters, rainfed upland clusters and coastal shore farming clusters. The constraints for farming and livelihoods in these clusters are identified as frequent floods, inundation, drought, weed problems, crop failure, and lack of diversification (Kathiresan, 2009a and Kathiresan, 2010). Farming systems that would be resilient to such climatic and farming constraints designed through rigorous institutional and on-farm experiments (Kathiresan, 2007; Kathiresan, 2009b and Kathiresan, 2012) were disseminated for adoption by the farmers in these clusters.

Farm economic efficiency of a specific area is an important factor of productivity and growth, where resources are scare and opportunities for developing and adopting better technologies have lately declining. No doubt, conventional farming is risky and farmers are reluctant to invert heavily in crop production. Modest increments in productivity are no longer sufficient to justify the investment of scarce resources as farming sector is small scale and resource poor, but integrated farming system assumes greater importance for sound management of farm resources to enhance the farm productivity, reduce the environmental degradation, improve the quality of life of resource poor farmers and to maintained sustainability. Farming system refers to the farm wherein two or more enterprises are integrated with the farm resources for achieving the fuller utilization, realize maximum profit and stabilize returns.

Various components of farming systems are as under

1. Cropping system

A system is set of elements which depend on each other and interacting among themselves. Farming system consists of several enterprises like cropping system, dairying, piggery, poultry, fishery, bee keeping, etc. These enterprises are interrelated. The end products and wastes of one enterprise are used as inputs in other. The wastes of cow, dung used as FYM in crop production and straw is used as feeding material for cow. Bullock is used for field preparation.

Cropping system is an important component of a farming system. It represents cropping pattern used on a farm and their interaction with farm resources, other farm enterprises and available technology which determine their makeup.

Cropping pattern: means the proportion of area under various crops at a point of time in a unit area. It indicates the yearly sequence and spatial arrangement of crops and fallow in an area. It indicates the early sequence and spatial arrangement of crops and fallow in an area. Crop sequence and crop rotation are generally used synonymously. Crop rotation refers to recurrent succession of crops on the same piece of land either in a year or over a longer period of time. Component crops are so chosen so that soil health in not impaired.

In agriculture, crop production is the main activity. However, the income obtained from crops may hardly be sufficient to sustain the farm family throughout the year. Assured regular cash flow is possible when the crop is combined with other enterprises. These other enterprises or components are called allied components.

Background

Farming system is a complex inter-related matrix of soils, plants, animals, implements, labour and capital, inter-dependent farming enterprises. The farm is viewed in a holistic manner (multi-disciplinary approach). Farming system approach requires commonly homogenous type of farmers. It is an inter-disciplinary, participatory and bottom-up planning approach. It is an approach for developing farm household systems. It is built on the principles of productivity, profitability, stability and sustainability which complement component oriented approach to development.

A farming system is composed of several farm activities, such as cropping systems, horticulture, livestock, fisheries, forestry, and poultry, as well as the tools the farmer has at his disposal to raise these things profitably. However, a lot of definitions generally express the same concept, which is that it is a technique to achieve profitable and sustainable agricultural production in order to meet the varied needs of the farming community while sparing the natural resource base and environmental quality.

In India since ages, the dryland farmers adopted a kind of mixed farming involving traditional annual crops and livestock consisting of one or two cows / bullocks and few poultry birds. This traditional system though of small scale provided some kind of risk minimisation particularly in the years of scanty rainfall causing agricultural drought. (https://coabnau.in/uploads/1587050523_ Agriculturalheritage.pdf) Discouraged by their marginal impact on farm economy, dryland farmers switched over to the lure of ‘productive’ mono-cropping with certain commercial crops like cotton etc. The continuous mono-cropping resulted in impoverishment of soils and low crop yields which took farmers into the labyrinth of poverty.

The arid regions of India, spread over 3,17,090 sq. km account for 12% area of the country. The western Rajasthan carries the onus of nearly 62% of arid area. Dryland farming is the main occupation of the people in Indian arid zone. More than 70% of the population in the region is engaged in dryland farming on more than 90% of cultivable lands. The average farm family in Indian arid zone consists of 6-8 members possessing 4-6 hectares of land. The over-exploited fragile natural resources are predominantly under mixed farming - pastoral as well as arable. Farming is almost entirely rainfed in this zone with low and unstable yields.

Farming system is an integrated set of activities that farmers perform in their farms with available resources and circumstances to maximize the productivity and net farm income on a sustainable basis. The farming system conceptually is a set of elements or components, i.e., soil, water, crops, livestock, labour, capital, energy and other resources that are interrelated and interact among themselves. At the centre of the interaction lies the farmer exercising control and choice regarding the types of results of interaction. The income of cropping alone from small and marginal farm is insufficient now to sustain the farmers family. A judicious mix of any one or more of these enterprises should complement the farm income and help in recycling the farm residues / wastes. The selection of enterprises must be based on the cardinal principles of minimizing the competition and maximizing the complementary between the enterprises. Of late, the researchers on multidisciplinary approach greatly realized and started developing the various farming systems models in accordance with the agro-eco systems zones.

Farming System thus may be defined as a complex interrelated matrix of soil, plants, animals, implements, power, labour, capital and other inputs controlled in part by farming families and influenced to varying degrees by political, economic, institutional and social forces that operate at many levels. The farming system therefore, refers to the farm as an entity of inter-dependent farming enterprises carried out on the farm. The farm is viewed in a holistic manner and the farmers are subjected to many socio-economic, biophysical, institutional, administrative and technological constraints.

Start with a 1:2 molar ratio of vector: insert DNA when cloning a fragment into a plasmid vector. This ratio will vary with other types of vectors, for example, cDNA and genomic cloning vectors. The following example illustrates the conversion of molar ratios to mass ratios for a 3.0kb plasmid and a 0.5kb insert DNA fragment.

Introduction
The integration of bioinformatics into plant breeding has been shaped by several key milestones over the past few decades. Concepts like Breeding 4.0 (Wallace et al.,2018) and, 5G Breeding (Varshney et al., 2020) have gained global prominence, strengthened by the availability of extensive omics data. The advent of DNA sequencing technologies marked a key milestone in this field, catalyzing numerous genome sequencing projects worldwide such as Arabidopsis, rice, tomato, maize, wheat, etc. Next-generation sequencing (NGS) platforms, such as Illumina, PacBio, and Oxford Nanopore, have enabled rapid sequencing of entire plant genomes, facilitating the identification of genetic variations, including single nucleotide polymorphisms (SNPs), insertions, deletions, and structural variants (Deschamps & Campbell, 2010; Fuentes-Pardo et al.,2017). These advancements have significantly improved comparative genomics, enabling researchers to compare the genetic makeup of different plant species or varieties and identify genes associated with agronomic traits, such as yield, disease resistance, and environmental stress tolerance (Khan et al., 2024). As a result, these developments have revolutionized plant genetic research and crop improvement strategies, providing unprecedented opportunities to enhance agricultural productivity (Bellare et al., 2018; Shendure and Ji, 2008).
Bioinformatics has revolutionized plant biology by combining computational and statistical tools to analyze genomes, transcriptomes, proteomes, and metabolomes facilitating insights into genome structure, function, and evolution (Yadav et al., 2015; Saha et al., 2023; Roychowdhury et al., 2023). Genomics, emerged in the 1980s primarily focussing on mapping and sequencing genomes and there after laid the groundwork for functional genomics, which systematically explored gene functions (Hieter and Boguski, 1997; (Jackson et al., 2011). Further, advances in sequencing technologies, such as long-read platforms, have enabled detailed analyses of structural variants in crop genomes, crucial for identifying agronomic traits.
The large volumes of genomic sequence data have allowed researchers to identify genetic markers for crop improvement and candidate genes for trait development. However, the full potential of these genomic sequencing efforts depends on assigning functions to the thousands of genes with unknown roles. Model organisms such as humans, mice, Arabidopsis, rice, tomato, Medicago, and wheat have been instrumental in advancing genomic investigations (Bult, 2006). A notable example is the model organism Arabidopsis thaliana, which has provided a foundation for exploring fundamental biological phenomena using resources such as the TAIR database (Lamesch et al., 2012). Leveraging advanced bioinformatics techniques, researchers have enhanced crop productivity, mitigated diseases, and optimized resource management in agriculture. Advanced approaches like genome-wide association studies (GWAS) and transcriptome analysis continue to refine the understanding of genetic underpinnings, revolutionizing modern agricultural strategies (Mai et al., 2023).

Abstract

FASTA, which stands for “Fast All”, is a sequence alignment tool developed by William R. Pearson and David J. Lipman in 1985. It is commonly used to identify similarities between DNA and protein sequences. FASTA employs a “hashing” strategy to locate matches for short stretches of identical residues with a length of k, referred to as k-tuples or k-tups. This method enables FASTA to quickly search large databases for sequence similarities, making it a popular tool for identifying homologous sequences, predicting gene functions, and analyzing evolutionary relationships. This chapter elaborates some basics of FASTA.

Maintenance

When an animal is being fed for growth, fattening, milk secretion or other productive function, a considerable part of its feed is utilized for supporting body processes which are vital, irrespective of new tissue or product is being formed. This demand for feed is referred to as the “Maintenance requirement”; because it includes the amount needed to keep intact the tissues of animal which is not growing, working or yielding any product. There would be tissue break down with the accompanying loss in weight, leading to undesirable consequences, provided such need for feed is not met.

The fasting catabolism

The fasting animal doing no external work and not producing in any way is still carrying on a variety of internal physiological processes, which are absolutely vital for life. Obviouslythe nutrients required to support theseinternal activities must come from the break down of body tissue itself. This destruction of body tissue is termed as the “Fasting catabolism” and this can be measured in terms of the waste products discarded through the various paths of excretion.

Energy catabolism of fasting

The energy expended in the fasting animal is converted into heat, which can be measured in direct or indirect calorimeter. For measuring fasting catabolism at its minimumvalue, it becomes imperative to eliminate all the influences leading to increase heat production above the minimum expenditure compatible with the maintenance of life in so far as possible. Such a minimum value is called “Basal-metabolism” or BMR.Theconditions essential fora true minimumvalue of fasting catabolism can most nearly be attained in human being are specified as following :

1. Lecithin is an example of
   1. Carbohydrates
   2. Hormones
   3. Vitamins
   4. Phospholipids
2. Which of the following acts as storehouse of energy in body?
   1. Hormone
   2. Vitamins
   3. Fats
   4. All of these

A. Answer the following questions

1.    What is omega carbon atom?

Ans: The methyl carbon atom at the distal end of the fatty acid chain is called omega carbon atom

2.    Why mammals can not synthesize essential fatty acid?

Ans: Because of their inability to introduce double bond between the 9th carbon atom and the terminal methyl group of the fatty acid chain.

3.    What is acid value?

Ans: It is defined as the number of milligram of KOH required to neutralize the free organic acids in 1 gm of fat.

4.    What is saponification number?

Ans: It is defined as the number of milligrams of KOH required to neutralize the free fatty acid and saponify the esterified fatty acids in 1 gm of fat.

5.    What is iodine number?

23.1 Introduction
Various products can also be produced by emulsions that contain fat globules. For instance, cream is produced when milk contains concentrated fat. This cream can be further processed to produce butter, ghee, and butteroil. “Milk fat, ghee, butter oil, anhydrous milk fat, and anhydrous butter oil are fatty products that are exclusively derived from milk or products obtained from milk, or both, through processes that result in the almost complete removal of water and milk solids, not fat.” The method of manufacturing (FSSAI standards) has notably influenced the flavour and physical structure of ghee.

23.2 Types of Fat Rich Dairy Products
23.2.1 Cream or Malayi
“Cream is a yellowish component of milk that naturally rises to the surface when milk is allowed to stand. It is rich in fat globules.” Cream is mechanically separated in the dairy industry. The size of the fat globules is diminished as a result of homogenisation of cream, which renders the product less suitable for whipping.
Cream can be defined as
1. That portion of milk, which is rich in milk fat
2. That portion of milk into which has been gathered and which contains large portion of milk fat
3. When milk fat is concentrated into a fraction of the original milk that portion is called cream.

Assay of Carotene, Tocopherol and Plant Pigments (Method of Astrup et al., 1971). Agricultural University of NORWAY, ÅS-NLH, NORWAY

Introduction

A method which assays the carotene together with chlorophyll a and b and xanthophyll was given by worker (1957, 1958) and this was a successful approach towards a useful routine procedure. Worker’s way of extraction is based on the use of aqueous acetone a technique which was studied by Davidson (1954). The separation is accomplished on an alumina column. Worker (1958) has used a double successive chromatography, once on magnesia and again on alumina, to obtain its separation from the other lipid components. The method developed by Astrup et al. (1971) permits simultaneous assay of tocopherol, carotene and plant pigments using alumina column.

Fat-rich dairy products such as cream, makhan, butter, butter oil and ghee have played a pivotal role in growth and development of dairy industry in India as well as abroad from the time immemorial. The reference of makhan and ghee has been found in old Indian scriptures. Makhan was popularized by Lord Krishna about 5000 years before. In the traditional Indian dietary regimen, milk fat in the form of malai (cream), makhan (freshly churned butter) contributes significantly towards nourishment of people of almost all ages.

Indian dairy industry, over the years, has been converting surplus milk for the manufacture of fat-rich dairy products, especially ghee and butter with skimmilk as a byproduct because of technological and economic reasons. More than one-third of the total milk production is being utilized for the production of ghee and butter wherein milk lipids, the most expensive constituents of milk are conserved and preserved. Milk lipids play many diverse roles, some of which are essential for human health. Many of the desirable flavour and textural attributes of dairy products are due to their lipid components, consequently, butterfat has, traditionally, been highly valued. Significance of fat can also be realized from the fact that the consumer’s perception of food quality is largely based on the perceivable rich taste. Unfortunately, milk lipids are subject to chemical and enzymatic alterations which can cause flavour defects referred to as oxidative and hydrolytic rancidity, respectively. The storage stability of high-fat products is strongly influenced by these changes.

9.1 Introduction

Nanoparticles (NPs) are particulate matter with at least one dimension less than 100 nm (Christian et al., 2008). Although NPs are naturally present in the environment, manufactured NPs may have distinctive surface properties and chemistry in comparison with natural NPs (Handy et al., 2008). Manufactured NPs have a large area-to-volume ratio and size-dependent properties, which impart novel properties and behaviors that make them suitable for nanotechnology applications (Auffan et al., 2009). Range of applications for NPs has brought about a rapid development of the nanotechnology industry.

When a herbicide is applied to the soil, it is subjected to various interactions with soil and surrounding environment which ultimately determines its persistence. Dissipation of these compounds from soil system is by three main processes viz., physical (volatilization, leaching and runoff), chemical (photochemical decomposition, adsorption by organic and inorganic colloids and chemical decomposition) and biological (microbial decomposition and plant uptake) 

1. Physical Processes

a) Volatilisation

Lipids are a diverse group of chemical molecules. They are generally insoluble in water but soluble in organic solvents. Lipids include long-chain hydrocarbons, alcohols, aldehydes, fatty acids, and their derivatives, especially, glycerides, phospholipids, glycolipids and sulfolipids. Other compounds like sterols, fatty acid esters of sterols, vitamins A, D, E, and K, carotenoids, and other poly-isoprenoids also are part of the lipids. Oils and fats are mainly triglycerides and neutral molecules. Those in the liquid state at ambient temperatures are called oils while those in the solid state are called fats. Lipids and lipid components have varied uses in the food and non food sectors. Vegetable and animal matter contain a variety of these constituents in different proportions. It is neccessry to evaluate the composition and quality of these constituents from the point of view of their utility.

Procedure: Measurement of smoke point of given oils: Heat the given oils in a deep frying pan till fumes come out from oil. Record the temperature when the fumes come out and noted it as a ‘smoke point’. Cool the oil by keeping oil pan at room temperature. After cooling repeat the heating of same oils 2nd and 3rd time to measure the smoke point and record the observations

Fats are obtained from both animal and vegetable sources. The principal animal fats are cream, butter, egg yolk, the fat of beef, mutton, pork, bone marrow, chicken fat, and codliver oil. Vegetable fats are those derived from fruits and seeds of plant, as olive oil, cottonsed oil, corn oil, peanut oil, soyabean oil and nut oils.

COMPOSITION AND NUTRITIVE VALUE OF FATS

Fats and oils, the most concentrated of fuel foods, were the first of the organic compounds or foods to have their composition determined. They are made up of same three elements which go to produce the carbohydrates, but the oxygen is less in proportion to the carbon. This explains the fact that fat yields more energy per gram than carbohydrates do.

Lipids are a class of compounds that are defined by how they are isolated, rather than by their composition. Lipids are molecules that can be extracted from plants and animals by low polarity solvents such as ether, chloroform, or even acetone. They are not appreciably soluble in water. Fats and the fatty acids from which they are made belong to this group as do oils, waxes and steroids. Hydrolyzable lipids are those that contain a functional group that will react with water. The functional group is usually an ester and the list of compounds includes neutral fats, waxes, phospholipids, and glycolipids. Non- hydrolyzable lipids lack such functional groups and include steroids and fat-soluble vitamins (e.g. A, D, E, and K).

Fats and oils are composed of triacylglycerols. These are compounds prepared by the union of glycerol (1,2, 3-tri- hydroxypropane) and 3 fatty acids to form a triester. Fats and oils belong to a group of biological substances called lipids. Lipids are biological chemicals that do not dissolve in water. They serve a variety of functions in organisms, such as regulatory messengers (hormones), structural components of membranes, and as energy storehouses. Fats and oils generally function in the latter capacity. Fats differ from oils only in that they are solid at room temperature, while oils are liquid. Fats and oils share a common molecular structure. The fats and oils contain three ester functional groups. Fats and oils are esters of the tri- alcohol, glycerol (or glycerine). Therefore, fats and oils are commonly called triglycerides, although a more accurate name is triacylglycerols. Triglyceride molecules contain mostly carbon and hydrogen atoms, with only six oxygen atoms per molecule. This means that fats and oils are highly reduced (un-oxidized). Oils are particularly common in seeds, where the stored energy helps seedlings during germination, until they can exploit solar energy through photosynthesis. Fatty acids contain an even number of carbon atoms, from 4 to 36, bonded in an unbranched chain. Most of the bonds between carbon atoms are single bonds. If all of these bonds are single bonds, the fatty acid is said to be saturated, because the number of atoms attached to each carbon atom is the maximum of four. If some of the bonds between carbon atoms are double bonds, then the fatty acid is unsaturated. When there is only one double bond, it is usually between the 9th and 10th carbon atom in the chain, where the carbon atom attached to the oxygen atoms is counted as the first carbon atom. If there is a second double bond, it usually occurs between the 12th and 13th carbon atoms, while a third is usually between the 15th and 16th.

Fats are rich sources of energy. Fats provide 2.25 times more available enrgy than carbohydrates. Fats also increase the palatability and reduce the dustiness of feed. Fats and fatty acids decrease the heat increment in the body. Fatty acids are also involved in number of physiological functions of the body. Recently, considerable interest has been shown on studies pertaining to dietary supplementation of polyunsaturated fatty acids (PUFA) in animal feed and their role in animal health and production.

Fatty liver, also known as hepatic lipidosis is a metabolic disorder commonly seen in various animal species. It occurs due to excessive accumulation of fat (lipids) in liver cells (hepatocytes), leading to impaired liver function. This condition is particularly prevalent in dairy cattle, poultry, cats, and occasionally in dogs and other species. Fatty liver can significantly impact health, productivity, and overall well-being. Nutritional management plays a crucial role in both preventing and treating this condition. Fatty liver occurs when the liver’s ability to metabolize and export fat is overwhelmed, leading to fat accumulation. This can happen due to various factors, including: Imbalance in energy metabolism Excess energy intake or inadequate utilization of energy during critical periods (e.g., early lactation in dairy cows) results in fat mobilization to the liver.
Obesity
Overweight animals are more prone to developing fatty liver due to an increased baseline fat deposit.
Nutritional deficiencies
Lack of essential nutrients like choline, methionine, and B vitamins can impair lipid metabolism. 

India’s vast geographical expanse and diverse climatic conditions have led to the development of distinct agro-climatic zones. These zones, as defined by the Planning Commission and ICAR, provide a scientific basis for selecting location-specific farming systems that ensure sustainable productivity, livelihood security, and efficient resource use. The feasibility of farming systems in these zones depends on factors such as rainfall, temperature, soil type, water availability, cropping intensity, and socio-economic conditions. The feasibility of different farming systems significantly depends on the specific agro-climatic zone, which encompasses factors such as temperature, rainfall, soil type, and elevation. India’s vast and varied geography is divided into 15 agro-climatic zones by the Planning Commission. Each zone has specific soil types, climatic conditions, topography, and resource availability. These factors influence the most feasible and sustainable farming systems. Below is a detailed description of suitable farming systems for each zone.
1. Western Himalayan Region (Jammu & Kashmir, Himachal Pradesh, Uttarakhand)
• Climate: Cold temperate to alpine.
• Feasible Farming Systems:
º Horticulture-based (apple, pear, walnut)
º Agri-horti-pastoral (wheat/barley + fruits + sheep/goat)
º Apiculture and sericulture
• Justification: Hilly terrain and climate favor fruits and small livestock.

Introduction India is a country of festivals and celebrations. Indian sweets enjoy a legendary reputation across the world. India is home to a number of delicious sweets that play a vital role in every Indian celebration. While gulab jamuns, rasgullas and kaju katlis may be the most popular sweets in the country (Pal Sanchari, 2016). Functions and events in India are celebrated with intake of sweets. Also, it is customary to sweeten the mouth in social gathering like marriage, birthday, poojas, etc. (Gulati and Misra, 2014). In the past few years, guidelines are formulated based on analyses of all published scientific studies on the consumption of sugars. In view of the rising prevalence of overweight, obesity and related disorders globally, in 2002, the WHO recommended that sugar should be less than 10 percent of daily calories. The consensus dietary guidelines for Indians in 2011 recommend less than 10% of total calories from free sugars per day. In May 2014, the expert panel of WHO recently recommended decreasing sugar intake to 5% of total calorie intake to combat obesity. A high intake of added sugars is a primary risk factor for diabetes. It is these added sugars in sweets that makes them palatable, but also does the physical damage. (Willet Brian, 2011). People have developed taste towards food in which fat and sugar contents are high.

There are several types of features and structures of the gardens. The design, material and placement varies from one type of garden to others. However, there are some basic requirements in every type of gardens. Common features and structures of the gardens are described hereunder for providing some basic information.

Boundary

Material and construction of the outer boundary is to be considered depending upon the size and type of garden. Protection, permanency and cost of construction are the main factors. It should be well designed and an architectural show piece co-relating with type and design of the garden. Ornamental creepers are planted along the boundary wall and trained over the same for further beautification purpose.

A. Answer the following?

1.    How antibiotic increases nutrient absorption from the gut?

Ans: In antibiotic fed animals, the intestine becomes thinner and healthier, thus they helps in more absorption of nutrient.

2.    What is probiotic?

Ans: Probioticcan be defined asthe live microbial food supplement that beneficially affects the host animal by improving the intestinalmicrobial balance.

3.    What is prebiotic?

Ans: These are the compound other than dietary nutrients that modifythe balance of microbial populationbypromoting the growth of beneficial bacteria and thereby maintain healthier intestinal environments.

4.    What is eubiotics?

Ans: Eubiotics are defined as non-antibiotic products maintaining the desiredbalance of good bacteriaand pathogens or eubiosis in the digestive tract. e.g. direct acting gut flora modulator (organic acid, essential oil), probiotics, prebiotics, immune modulator.

5.    What is supplement?

Feed processing includes all operations necessary to achieve the maximum potential of nutritional value of a feed stuff. The process involves changing ingredients in such a manner as to maximize their natural value and the net returns from their use. Feed processing may be accompanied by Physical, Chemical, Thermal, Bacterial or other changes of a feed ingredient before it is fed. The primary reasons for processing feeds are to make changes in the moisture content, density of feed, particle size, palatability; or to make more profit, to improve nutrient availability, keeping quality (shelf – life); or to reduce storage – transportation space, cost, moulds, Salmonella and other harmful substances. Feed mill equipment: The milling industry has been concerned with “Grinding” – a process of particle size reduction. During
earlier times, the same equipment was used Food for humans to produce Food for livestock because, the process was basically ‘grinding’ of whole grains. As milling developed into the “Modern Flour Industry”, the milling process was extended to include:

The proper supply of feed and fodder is a requirement for the growth and development of the animals. Over the past few decades, there has been a considerable growth in milk production, with India now producing the most milk in the world (187.7 million tones in 2018–19). The main causes of India’s low milk yield are extrinsic (poor nutrition/feed management) as well as intrinsic (low genetic potential).

Current status of feed and fodder availability in India Globally India ranks 1st in terms of total livestock (535.78 million) and buffalo population in the world (109.85 million), 2nd in cattle (192.49 million), goat (148.88 million) and 3rd in sheep population. So, the availability of adequate quantity and quality feed and fodder resources for livestock is essential for improving livestock productivity. Grazing lands and fodder cultivation areas are gradually dwindling either due to degradation or restriction for livestock grazing. Total area under fodder cultivation is around 8.4 mha (5.23%) which is almost static for the past two decades. Even though feed and fodder availability has improved in the last decade still gap exists with demand and availability of fodder during lean periods and abnormal weather conditions like droughts/floods. At present, India faces a net deficit of 35.6% green fodder (GF), 10.95% dry crop residues and 44% concentrate feeds. In India, open forest cover is around 29 million hectares (mha), which can be further utilized for growing fodder trees without affecting standing trees, mostly as an under- storey on the partially shaded ground Area under fodder production Sorghum (2.6 M ha) and Egyptian clover (1.9 M ha) account for roughly 54% of the total cultivated fodder area in the kharif and