eChapter Name: Future Perspectives in Bioprocess Engineering: Trends and Innovation
9789372199178
eBook Name: MOLECULAR AND TECHNOLOGICAL ADVANCEMENTS IN BIOPROCESS TECHNOLOGY
9.1 Introduction
Biochemical technology focuses on developing sustainable conversion of renewable materials like lignocellulosic wastes into value-added products with reduced emissions. The utilization of integrated bioprocess engineering for transforming carbon sources into diverse biotechnological products via microbial cells is an emerging field (Pais et al., 2016). Bioprocess engineering is a collection of thermodynamics, process simulation, bio-separations, microbiology, bioprocess kinetics, and systems engineering aiming for biomass conversion, biocatalysis-driven processing methods, and microbial processes. These processes can range from microscale to large-scale designs and systems (Liu et al., 2016) and generate value-added products such as biofuels like bioethanol, biodiesel, and biogas (Patel et al., 2022); in pharmaceuticals (Tsopanoglou et al., 2021), probiotics (Lei et al., 2024), textiles (Bahtiyar et al., 2021), bioplastics, and biopolymers (Pathom-aree et al., 2024). Reactor design, fermentation, kinetics, and optimization are integral to bioprocess engineering. Not always but the pretreatment methods for lignocellulosic waste and its improvement should be considered for an optimized bioprocess engineering as they are the carbon source during fermentation. Pretreatment methods are physical, chemical, biological, or hybrid of any three treatments used to acquire desired products. Cost-effective and relatively slow biological pretreatment needs microorganisms to convert lignocellulosic waste into useful desired products (Abo et al., 2019). Chemical pretreatment utilizes harsh chemicals to increase biomass degradation, porosity, and solid separation but unfortunately are rarely used due to the harmful effects (Arora et al., 2018). Physical pretreatment methods utilize mechanical pressure to break down biomass but are expensive and irregular. Pretreatment for source and strain acquisition is the initial step of upstream bioprocessing followed by reaction conditions and bioreactor design (John et al., 2020). The primary part of any upstream process is to produce the desired product like enzyme followed downstream processing such as purification of the value-added product. The value-added products are developed via fermentation in equipment known as Bioreactors. Different bioreactors being utilized recently in bioprocess engineering and biorefinery are membrane bioreactors that offer high productivity, low environmental impact, and high flexibility (Akkoyunlu et al., 2024); airlift bioreactors (Hernández- Acevedo et al., 2024); fixed bed/packed bed and fluidized bed bioreactors are being utilized for their potential of reduced expenses, simpler design, enhanced temperature regulation and homogenous mixing (Srivastava et al., 2022) (Figure 10.1). Despite their high production and safety, the traditional large-scale stainless-steel vessels batch-wise are often expensive, inflexible, and have long construction times. As an alternative to the costly route, single-use equipment was introduced. Since they are pre-sterilized and are readily accessible to purchase as needed, this disposal method offers great flexibility and lower investment costs by eliminating the need for sterilization. However, there is high waste generation leading to a dent in sustainability and circular bioeconomy (Frank, 2018). The transformative step of the production system shifting from batchwise to continuous production has ultimately led to a decreased environmental footprint and prices and increased product quality and yield (Gerstweiler et al., 2021). The cost of bioprocess engineering (upstream and downstream) is dependent on factors such as types of feedstocks, fermentation types like submerged and solid-state, product types, and microbe or enzyme types (Kumar
