eChapters

Introduction

Agriculture is the mainstay of the Indian population and contributes about 30 per cent to the gross domestic product of India. The government has laid great emphasis onimproving productivity, quality of produce and welfare of farmers through awareness campaign and enhanced agri-knowledge for the farming community to double the farmer’s income by 2022 and make the farmers realise their significant contribution to the nation building. Doubling the farmer’s income can only be possible, with proper diversification of crops, developing the existing irrigation system in proper utilization of available water on plant requirement basis, conservation of soil fertility through adding organic manures to the field, best utilization of human excreta from the storage tanks of individual toilets after proper decomposition through bacterial degradation, fulfilling the nutrient requirement of crops on the basis of soil test values, proper financial support from the Government to the farmers in managing stress due to natural calamities etc. The governmentbelieves that farmers’ welfare will improve if there is increase in net income from the farms. With this end in view, the approach is to reduce cost of cultivation, enable higher yield per unit area and realize remunerative prices of farm produce. To achieve this goal, action on three pillars needs to be taken.

Introduction

It is a well known fact that agricultural pests cause substantial crop losses throughout the world. In the past farmers had to manage this problem to secure their basic subsistence needs, and as a response, the farmers practiced and developed cultural and mechanical pest control based on trial and error. Over a period of time, these practices have become a part of their production management system. The first known chemical control dates back to 2,500 years. From the late 1800s to 1940s, the main insecticidal compounds used were oils, soaps and resins, plant-derived poisons and inorganic compounds. The chemical age began in the 1940s with the discovery of dichloro diphenyl trichloroethane (insecticide), ferbam (fungicide), and 2, 4-D (herbicide) (Arneson and Losey, 1997). In 1940s and 1950s, pesticides were thought to be the final word in pest control and their introduction contributed substantially to raising agricultural productivity in many regions of the world. Since then, pesticides have become an integral component of many intensive agricultural systems. Repeated applications of pesticides lead to resistance in the pest (Beaument, 1993). This resistance results in the increased application of pesticides and to the collapse of the agricultural systems characterized by highly resistant pests, with no natural enemies left to control them. Pesticides have been found to causeacute and chronic human health problems, contamination of groundwater, surface water, atmospheric contamination, and negative effects on non-target organisms (Mullen, 1995). Concerns about the negative effects of pesticides led to research and promotion of alternative pest control practices – Integrated Pest Control or simply IPC. This new concept called Integrated Pest Control (IPC) and later Integrated Pest Management (IPM) was stimulated by symposia organized by the Food and Agriculture Organization (FAO) of the United Nations in 1966. IPM made a paradigm shift in the philosophy of pest control, from pest eradication to pest management. Instead of single tactic control, emphasis was placed on the use of a combination of available tactics in a compatible manner aimed at providing cheap, long term sustainability with minimum of harmful side effect and controls the pest at ETL level.

Introduction

Arid regions are characterized by stressful edaphic and environmental conditions such as low and erratic rainfall coupled with limited irrigation resources, high evapotranspiration and extreme temperature regimes. Under such environment, the productivity of agriculture crops is far behind the satisfactory level, rendering cultivation of field crops an unprofitable venture. The recent awareness about potential of ecologically fragile lands for cultivation of fruit trees has not only opened up scope for providing economic sustainability for the natives, especially small farmers, but also for bringing new areas under fruit production. Ber (Ziziphus mauritiana Lamk.) or Indian jujube, which belongs to the family Rhamnaceae and said to be indigenous fruit crop. Ber is grown in India traditionally from ancient times where it has been in use for almost 4000 years (Mittre, 1961). It is one of the most suitable fruit trees for arid and semiarid regions. It is also known as ‘King of Arid Fruits’, Chinese date, Chinese fig or poor man’s fruit. Ber can do well even under marginal growing conditions and provides quality yields at low cost (Shukla, 1996). Cultivation of ber under the harsh conditions of the Thar desert of India (west Rajasthan) demonstrates its adaptation to desert conditions. Flowering and fruiting of ber coincide with the availability of maximum rain water (monsoon rains), which is during JulySeptember. It has a long taprootand can withstand high temperatures during the summer. During the dry hot summer (AprilJune), it undergoes dormancy by shedding its leaves, thus evading the injury of drought. It can produce fruits even under rainfed situation where average annual rainfall is 150200 mm. It is truly a desert apple for the dwellers of Thar Desert. The tree propagates freely and greatly resists stress conditions in regions experiencing recurrent droughts. It is thus an important tree suitable for integration into the agroforestry systems in the warm desert ecoregions. The tree can provide economic sustenance to the region and insurance against ecological degradation.

Introduction

Pomegranate is one of the most remunerative fruit crops that grow well under arid and semi-arid agro eco-system with severe scarcity of water. The popularity of pomegranate has grown tremendously due to its health beneûts, high returns on investment, less water requirement, therapeutic and functional properties. Growing health consciousness among the consumers and acquisition of super fruit status by pomegranate has made marketing of pomegranate fruits and derived value added products quite easy. As the result of all these benefits, India is witnessing a pomegranate revolution with more than 80 % expansion in area and 250 % increase in production during last one decade. However, there are many challenges to be overcome so as to double the income of pomegranate growers, these challenges include market glut due to unprecedented increase in production coupled with limited export resulting into suboptimal price of the harvested produce; intensification of diseases and insects and pest due to huge unplanned expansion; dominance of only one variety; very limited value addition; hampered export due to suboptimal quality, unwanted sprays, issue of pesticide residues and lack of sufficient number pesticide label claims in pomegranate; inadequate supply chain management. So, in order to overcome these challenges and to increase the profitability of pomegranate stakeholders, we need to focus on scientifically sound cultivation practices to reduce the cost of cultivation, breeding for improved varieties, improved packaging, transport, storage, marketing, supply chain management, facilitating and encouraging export, practicing and promoting climate resilient horticulture, formation of farmers’ cooperatives and farmers producers organization (FPOs), processing and value addition with the aim of total utilization and use of information and communication technologies. This article deals with opportunities and strategies in pomegranate for doubling farmers’ income through nursery management; improved horticultural practices; integrated disease, insets pests and nutrient management; use of information and communication technologies; creation of FPOs; processing and value addition.

Introduction

Dry-land horticulture has immense potential in providing nutrition rich food, social security and eco-restoration for the inhabitants of desert or rainfed and tribal land-areas of the north-western parts of India. Most of the recent past advances in Indian Horticulture are with production technology for favorable agro-climate, however, the vast and diverse land-scape of hot desert, arid, semiarid and tribal area is yet not getting logical thought for resource based horticultural development. The native crop–plants both annual and perennial are equally playing vital role for food, fodder as well income especially in the hot arid and sem-arid areas of Rajasthan where animal husbandry is also an integral component of the farming system. The monsoon supported arid zone crop-plants are drought tolerant and even the harvest failure in kharif season/ conventional cropping is very high and it is estimated 50–60% in most of the years only because of uncertainty in rain’s pattern. Based on my research experience and SWOT analysis over 25 years, it is accomplished that this hot arid zone has excellent horticultural potential with wide and varied opportunities of crop production, utilization of resources and eco-restoration (Samadia, 2016).

Introduction

Climate change has become one of the biggest challenges for the sustainable crop production. Prolonged droughts and desertification are among the issues faced by Indian hot arid zone where the rural poor and smallholders are most heavily affected. If people are to survive in these ever harsher conditions, their crops need to withstand drought, high temperatures and poor soils. Cactus crops are gaining increasing interest across the globe, in particular cactus pear or prickly pear {Opuntia ficus indica (L) Mill.} because of its unique characteristics which provide resilience to the harsh ecological conditions. Cactus pear is able to grow on land where no other crops are able to grow; it can be used to restore degraded land. It is the only crop that can be relied on when everything else fails. Cactus pear is grouped as CAM plant (Crassulacean Acid Metabolism), a photosynthesis mechanism evolved in some plants as an adaptation to arid conditions. In a plant using full CAM, the stomata in the leaves remain shut during the day to reduce evapotranspiration, but open at night to collect carbon dioxide (CO₂).

Introduction

Harvesting and handling are the equally important activities as the entire production system as an individual activity. Therefore, proper harvesting and handling is essential to assure the farm income. There are various factors which directly affect the storage life of fruits and vegetables as below. The natural limits to the post -harvest life of all types of fresh produce are severely affected by other biological and environmental conditions.

Temperature: An increase in temperature causes an increase in the rate of natural respiration of all produce and food reserves and water content become depleted. The cooling of produce will extend shelf-life by slowing the rate of respiration.

Transpiration and water losses: High temperature, low humidity in the store and injuries to produce can greatly increase the loss of water from stored produce beyond that unavoidable lost from natural causes. Maximum storage life can be achieved by storing only undamaged produce at the lowest temperature tolerable by the crop and at the relative humidity appropriate for the produce.

Mechanical damage: Damage caused during harvesting and subsequent handling (i.e. injuries, impact bruising) increase the rate of deterioration of produce and renders it liable to attacks by decay organisms even under refrigerated storage. Mechanical damage to root crops will cause heavy losses owing to bacterial decay and must be remedied by curing the roots or tubers before storage. Curing is a wound –healing process.

Decay in storage: Decay of fresh produce during storage is mostly caused by the infection of mechanical injuries provoked by micro organisms, mostly bacteria and fungi. Furthermore, many fruits and vegetables are attacked by decay organisms which penetrate through natural openings or even through the intact skin. These infections may be established during the growth of the plant in the field but lie dormant until after harvest, often becoming visible only during storage or ripening.

Introduction

The Indian arid zone covers nearly 31.8 million ha of the country’s geographical area. It mainly spreads over parts of Andhra Pradesh, Gujarat, Haryana, Karnataka, Maharashtra, Punjab and Rajasthan states of India. Six of the eleven districts in Western Rajasthan i.e. Barmer, Bikaner, Churu, ShriGanganagar, Jaisalmer and Jodhpur lie wholly in the arid zone. High temperature, low precipitation and high velocity winds are some of the main culprits of Arid zone which limit the scope for higher crop productivity in these areas. Under this situation, arid horticulture based farming practices has ample scope to raise the productivity and living standard of peoples of the hot arid and semi-arid regions. Arid horticulture based farming practices greatly favour the development of high quality produce in many fruit and vegetable crops. Horticulture based farming practices are now considered to be the most ideal strategy to provide food,nutrition and income security to the people especially in arid and semi-arid region of the country (Chundawat 1993; Chadha 2002).

Today India is the world largest producer of many fruits and vegetables. Wastes and by-products generated from fruits and vegetables are organic in nature and contribute a major share in soil and water pollution. Also, accumulated fruit and vegetable wastes (FVWs) cause greenhouse gas emission which is a matter of serious environmental concern. Proper disposal and handling of these wastes can help reduce pollution risks, at the same time be a source of several useful and commercially valuable by-products. Organized processing of wastes can help farmers achieve higher incomes.

Introduction

Any project is undertaken and an investment is made with expectation of realizing benefits out of it. This applies to projects both in the private domain as also in the public domain. Hence, it is imperative to assess the benefits derived from the project investment over its entire economically viable. The extent to which project contribute to various aims of development (benefits) and make use of scarce resource (costs) can differ widely. A proper project preparation requires an assessment of the various benefits and costs associated with execution of a project. Methods to determine, analyze and assess the relevant benefits and costs of a project are known as cost-benefit analysis. The cost- benefit analysis ensures that all the relevant factors that contribute to a project’s net benefits are systematically taken into account, whether they relate to an individual, a firm, a social group, the public sector or the country as a whole.

There are many tools or criteria for the financial evaluation of projects. However, the important and commonly used evaluation criteria are simple Rate of Return, Payback Period, Net Present Worth, Benefit- Cost Ratio and Internal Rate of Return.

Economic appraisal and evaluation helps the decision makers who are saddled with the responsibility to prioritize measures in a situation with enormous needs and quite limited resources. Government planners as well as donor agencies are all in need of enough data in order to make rational choice or decision in selection of investment alternatives. Most of the economists believe that economic analysis cannot give the final answers. There will always be lack of data, uncertainty about the future, disagreement on the methods, and similar issues. However, economic analysis, if properly done, can indeed be of much help to decision makers.

Introduction

Some historians of agriculture believe that it was women who first domesticated crop plants and there by initiated the art and science of farming. While men went out hunting in search of food, women started collecting seeds from the native flora and began cultivating those of interest from the point of view of food, feed, fodder, fiber and fuel. This view is strengthened by the fact that women have been traditionally seed selectors. Even today this tradition has continued in many parts of the developing world. Women have played and continue to play a pivotal role in the conservation of basic life support system such as land, water, flora and fauna. They have protected the health of the soil through organic recycling and promoted crop security through the maintenance of varietal diversity and genetic resistance. In many remote areas agriculture is largely in hands of women. Men tend use to go to towns and cities in search of salaried jobs for fulfillment of family income. Therefore, without total intellectual and physical participation of women, it will not be possible to popularize alternative systems of land management for shifting cultivation, arrest gene and soil erosion, and promote the care of the soil and the health of economic plants and farm animals. Women also tend to look at problems in their totality from the sowing of the crop to its ultimate utilization either as food, feed or raw material for industry. Since they are usually incharge of marketing, they generally possess a clear understanding of market preferences and prejudices. Therefore, for an economically and ecologically sustainable agriculture the involvement of women farmers and farm women (agriculture farmers with and without assets) in the process of modernization of farming practices and village industries is absolutely essential. Horticulture is a major part of Indian agriculture, natural foods of man, fruits and vegetables provide protective nutrients like vitamins and minerals besides, some high quality sugars, proteins, oils and fats. Although belated, their importance has now been recognized in India not only to provide the much needed balanced nutrition and gainful occupation to our populace but also to develop viable internal and export trade. Fruits like banana, mango and minors fruit like mahuva, chironji, rayan etc. vegetables like potato being energy rich are considered prominent food crops e.g. banana can produce as high as 37 million calories per hectare. Unfortunately the component of fruits and vegetables in Indian diet is hardly six percent which should be raised at least five times so that the per capita per day consumption becomes 85 g. fruits, 75 – 125 g. leafy vegetables 85 g. other vegetable and 85 g. roots and tubers. To achieve this present production of fruits and vegetables would need to be pushed up considerably by increasing production levels and the area under fruits and vegetables would need to be pushed up considerably by increasing production levels and the area under fruits and vegetables (Lata and Singh, 2010).

Introduction

The hot arid regions of India are spread over 32 million ha (0.32 million Sq.Km.) in the state of Rajasthan (61%), Gujarat (20%), Andhra Pradesh (7%), Punjab (5%), Haryana (4%), Karanataka (3%), and Maharashtra(0.4%) which are characterized by hostile agro-climatic conditions and fragile eco-system. Out of total hot arid area of the country (India), more than 60% area falls under the state of Rajasthan which covers 12 districts viz., Jaisalmer, Barmer, Bikaner, Churu, Sikar, Jhunhjunu, Naguar, Jodhpur, Pali, Jalor, Sriganganagar, and Hanumangarh of the state. In general, the hot arid region of Rajasthan receives very low rainfall, varies from 100 mm annun^-1in north-western district of Jaisalmer to 450 mm per annum in the eastern boundaries of arid district of Rajasthan. The potential evapo-transpiration varies from 2063 mm in Jaisalmer to 1503 mm in Sikar districts of Rajasthan. The rainfall is very erratic and often experienced prolong drought. The ground water table is very deep and often brackish in nature. The extremes of temperature having severe winter during December-January with temperature as low as -4 °C and very hot summer during May-June with temperature as high as 48-50 °C is another important characteristic of the hot arid regions. The solar radiation is very high. Atmospheric vapour pressure deficit reaches as high as 30 mb during summer (May-June). The wind velocity in June is very high which leads to heavy wind erosion and formation of sand dunes. Some time strong sand storms of very high speed (60-0 km h^-1) with huge amount of sand particles also occurs. The soils are coarse textured with low silt, clay and humus content and water holding capacity is very low and infiltration rate is very high. The soil fertility is very poor having low organic carbon (0.02-0.06%).

Introduction

The hot arid zones in India are spread over nearly 31.7 million hectare area of which 41.5 per cent is arable and 19 per cent is cultural wasteland. These zones are perpetuated mainly in North –West and Southern part of the country. The major parts of the country under hot arid climatic conditions are Western Rajasthan (19.62 Million ha), North-western Gujarat (6.2 M. ha), South-western Punjab (1.45 M. ha), South-western Haryana (1.28 M. ha), Andhra Pradesh (2.16 M. ha), Karnataka (0.86 M. ha), and Maharashtra ( 0.13 M. ha). These areas/ zones are characterized by hostile agro-climate and fragile eco-system, an annual rainfall between 100 – 500 mm with a coefficient of variation (CV) varying from 40 – 70 per cent, low and erratic rainfall combined with extremes of temperature (450-500 cal/cm²/day); low relative humidity; high potential evapo-transpiration value ranging from 1600 mm in eastern part and 1800 mm in western part of the hot arid zones. Other important characteristics of the hot arid regions are hot winds with high velocity, poor soil condition, poor ground water with brackish and saline in reaction, poor vegetation, frequent occurrence of drought and frost, difficult to execute agro-techniques, difficulty in post harvest handling and marketing owning to limited and inefficient transportation and marketing facilities, etc (Yadav and Soni, 2008).

Horticulture word derived from Latin word: Hortus meaning ‘Garden’ and cultura means ‘cultivation’.

Father of horticulture: Thomas Andrew Knight, John Lindley, Liberty Hyde Bailey.

1^st artificial hybridization in fruit crop in world: T. A. Knight.

Father of vegetables: L. H. Bailey.

1^st book in horticulture: Fruit growing in India: W.B. Hayes (1945) & related to litchi.

Export growth of fresh fruits and vegetables in term of value is 14% and of processed fruits and vegetables is 16.27%.

Contribution of horticulture sector to agriculture GDP (2013-14): 29%.

India is largest producer of Coconut, Cashew, Tea and Spices.

1. GENERAL POMOLOGY

Word pomology: Derived from Greek word ‘pome’ meaning development of fruit from inferior ovary and ‘logos’ meaning to study.

Father of systematic pomology: De Candolle.

Temperate fruits are popularly known in India as hill fruits or cold region fruits.

India ranks 1^st in productivity of grapes, banana and papaya.

Ratio of Chlorophyll a to chlorophyll b is highest in C⁴ plants.

Gibberellic acid is enhanced by exposing plants to red light.

The life line tree of ‘Thar Desert’ is Prosopis cineraria.

Grape of the desert is Salvadora oleoides.

1. General Olericulture

Olericulture is Latin term ‘Olerus’ meaning herb.

China, India, USA, Turkey and Italy: Major vegetable producers.

Indian subcontinent has 80 species of major and minor vegetables apart from several wild species consumed as vegetable.

About 175 different types of vegetables are grown in India, includes 82 leafy vegetables and 41 root (tuber and bulb) crops.

Cucurbits, taros, yams, brinjal and okra have rich diversity in Indian gene centre.

USA is leading importer of cucumber and gherkins, assorted canned vegetables and other dehydrated vegetables from India.

Germany leads in import of preserved onion, green pepper in brine and dehydrated onion flakes/powder.

Singapore leads in import of dried asparagus; Japan in dehydrated garlic flakes; UAE in dehydrated garlic powder; Bangladesh in peas and Switzerland in dried mushrooms including morels.

Mizoram is the largest producer of chow chow.

1. GENERAL FLORICULTURE

Development of Floriculture’ was recommended by Dr. P.P. Trivedi committee.

India ranks 2^nd in flower cultivation next to China.

The production of loose flowers almost tripled between the 8^th Plan and the 11^th Plan.

The annual domestic demand is valued at around 60 billion USD.

About 2,33,000 hectares across the country was used for floriculture, producing 17,29,000 metric tonnes (MT) of loose flowers and 76,732 lakh cut flowers.

India has exported 22,947.23 MT of floriculture products to the world for worth of Rs. 460.75 crores in 2014-15.

The share of exports of dried flowers and plant parts are 45%, flowering plants (22%), fresh foliage (13%), fresh cut flowers (10%), live plants (7%) and bulbs and tubers (3%).

India’s share in global floriculture trade- 0.6%.

1. GENERAL INFORMATION

International Group for the Genetic Improvement of Cocoa (INGENIC) formed in 1993.

Central Coconut Research Station (presently CPCRI) was established in 1940.

Central Plantation Crops Research Institute (CPCRI), Kasargod, Kerala established in 1970.

CPCRI hosts International Coconut Gene Bank for South Asia under the umbrella of Coconut Genetic Resources Network (COGENT) of the Biodiversity International.

National Active Germplasm Site for coconut: CPCRI.

United Planter’s Association of Southern India (UPASI) started in 1893.

Branch of International Rubber Association was established in 1948 at Kottayam, Kerala. It was renamed as Indian Rubber Institute in 1987.

Rubber Research Institute of India, Kottayam established in 1995.

1. GENERAL INFORMATION

Asian continent, particularly India is known as “Land of Spices”.

Spice crops are largely grown in tropics between 20⁰N and 20⁰S.

Term ‘spice’ was derived from Latin ‘Species aromatacea’ means ‘Fruits of the earth’.

Spices and condiments are grouped under food adjuncts.

India is largest producer of spices and oleoresin.

Home of spices/ Spice bowl of India: Kerala.

1. GENERAL INFORMATION!

Major constraint of medicinal plants: Lack of genuine planting material.

Maximum number of medicinal plants belong to family Asteraceae.

Maximum demand in world market: 1. Senna leaves 2. Isabgol seeds 3. Cassia tora seeds.

India is largest producer of 1. Kewada oil 2. Senna 3. Davana oil 4. Isabgol.

India is 2^nd largest producer of 1. Opium latex 2. Jasmine concrete 3. Tuberose concrete

India is 3^rd largest exporter of periwinkle.

Thailand is largest exporter of sarpagandha leaves.

China is largest producer of 1. Geranium oil 2. Citronella oil.

1. GENERAL INFORMATION

India’s exports of Processed Food was Rs. 31563.43 Crores in 2014-15, which includes products like Mango pulp (Rs. 841.39 Crores), Dried and Preserved vegetable (Rs. 847.11 Crores), Other Processed Fruit and Vegetable (Rs. 2,569.93 Crores), Pulses (Rs. 1,209.51 Crores), Groundnuts (Rs. 4,675.35 Crores), Guargum (Rs. 9,480.00 Crores), Jaggery and Confectionary (Rs. 1,161.81 Crores), Cocoa Products (Rs. 848.62 Crores), Cereal Preparations (Rs. 3,038.79 Crores), Alcoholic and Non-Alcoholic Beverages (Rs. 2,231.58 Crores) and Miscellaneous Preparations (Rs. 2,437.77 Crores).

1. Introduction

During the last decade, considerable attention has been focused on the involvement of oxygen free radical (OFR) in various diseases. Molecular oxygen, while providing an efficient energy from ingested food, results in free radical and peroxide by-products, which have high intrinsic toxicity. Numerous physiological processes in living organisms occasionally produce free radicals, reactive nitrogen species (RNS) and reactive oxygen species (ROS) as by-products. Their broad ranges of effects in biological systems have drawn the attention of many experimental works. ROS have been implicated in the pathophysiology of several clinical disorders, including ischemia, reperfusion injury, myocardial infarction, rheumatoid arthritis, neurodegenerative, atherosclerosis, acute hypertension, hemorrhagic shock and diabetes mellitus (Hemnani and Parihar, 1998).

Oxidative stress occurs in a cell or tissue when the concentration of reactive oxygen species (ROS) generated exceeds the antioxidant capability of the cell (Simic and Jovanovic, 1986). Although humans and other organisms possess antioxidant defense and repair systems to protect against oxidative damage, these systems are insufficient under certain conditions to prevent the damage totally (Chang and Miles, 1982). Hence, the oxygen free radicals mediated damage to protein, DNA and other biomolecules accumulates during the lifetime of organisms. Many synthetic antioxidant components exhibited toxic or mutagenic effects that have directed the attention to the naturally occurring antioxidants in recent years. A multitude of natural antioxidants have already been isolated from different plant materials such as oilseeds, cereal crops, vegetables, fruits, leaves, roots, spices and herbs (Ramarathnam et al., 1995).

ABSTRACT

A plant grows in a highly competitive environment. It is continually being threatened by other plants encroaching upon the space from which it draws its sustenance, by microorganisms, by insects, avian, reptilian or mammalian herbivores. In order to survive, each plant must draw upon a complex of defenses, which may be physical, such as Spines or Leathery Leaves or Chemical. These defenses are passive, coming into action only when the plant is attacked which repel insects or the herbivores that attempt to feed on them. Plants protect themselves from insect attack by providing a multitude of repellent chemicals including toxic and bitter alkaloids and tannins. Non-specialist insects and other animals that attempt to feed on the plant may be repelled, injured through severe gastrointestinal malfunction or even killed. The cabbage, spinach, mustard etc. are well known for their mustard pungent flavor that deters most animals from consuming it. Even most medicinal plants like Cynanchum collialatum of the family Asclepediaceae have tannins or alkaloids like “cardenolides” that repel most animals and insects. All these plants have a basic enzyme “Myrosinase” which react with these specific tannins to produce the toxic metabolite, which repels insects and animals. But caterpillars of few insects are known to tolerate these toxic alkaloids (ex: caterpillar of Striped Tiger Butterfly-Danaus genutia) and in turn sequester the toxic alkaloids into their tissues making themselves non-palatable to their enemies. We hypothesize the analysis of possible roles of toxic alkaloids isolated from these butterflies as bio-insecticides

ABSTRACT

Senna (Cassia angustifolia), a traditional medicinal crop in Tamil Nadu warrants the modern quality guidelines viz. Good Agricultural Practices (GAP) for marketing overseas. Buyers of developed countries insist on GAP and organic certification for medicinal plants. With this in view, an experiment was conducted at Medicinal Plants Unit, Botanic Garden, Tamil Nadu Agricultural University, Coimbatore to study the effect of various biostimulants on yield and yield attributing characters to standardize the biostimulant(s) for higher yield. The treatments included different concentrations of panchakavya (2% & 4%), moringa leaf extract (2 & 4%) and humic acid (0.2 & 0.4%). The field was laid out in randomized block design. Vermicompost @ 4 t ha^-1 was applied commonly to all the plots. Spraying with the above said chemicals was done at 15 days interval from 30 days after transplanting for four times. The observations were recorded for growth a yield characters and were statistically analyzed. The treatment 2% panchakavya + 2% moringa leaf extract + 0.2% humic acid recorded the highest value of growth and yield characters like plant height (44.58 cm), number of branches (6.1), number of pods (53.0), dry leaf yield (560.8 kg / acre) and dry pod yield (6. 72 g / plant).

A new buzz-word is rapidly spreading through the manufacturing and analytical environment. Discussions on the application of Non Invasive Research namely the Near Infrared (NIR) Spectroscopy are often found on the agenda of production, project, and technical and even board meetings. Engineers and chemists are confronted more frequently with the challenge to decide whether a NIR instrument will be the solution to their production quality control and other analytical problems. Are there really amazing machines that can determine protein, fat, moisture, fibre, starch, nicotine, alcohol, sugar, amino acids and whatever analyses you require on any product from foodstuff to fertilizer and animal feeds in less than a minute and so simple to operate that even the unskilled labourer can handle it? In this article we will endeavor to answer the questions that often crop up in the evaluation of NIR as an analytical technique towards Natural Products.

ABSTRACT

Colchicine, one of the most widely used alkaloids is synthesized in Gloriosa species, a plant of great medicinal importance. The seeds, tubers and leaves of six different species of Gloriosa were phytochemically investigated for alkaloids and were found to contain colchicine and colchicoside. These alkaloids were isolated and quantified by HPLC analysis. This is the first report of alkaloids in Gloriosa plantii, Gloriosa lutea, and Gloriosa vuchuria. The colchicine and colchicoside content of the Gloriosa species was found to be organ dependent as there was statistically significant differences (P<0.05) between different organs but not among different species under study. The total alkaloids are organ as well as species dependent as there were statistically significant differences (P<0.05) between all the species as well as organs of different species of Gloriosa under study and its organs.

ABSTRACT

Diabetes is a chronic endocrinologic disorder characterized by high blood levels of glucose due to insufficient secretion of insulin by the pancreas or improper utilization of insulin by target cells. More than 120 million people from all over the globe are estimated to suffer from this malady. Many plants and their constituents have become chief ingredients of a number of pharmaceutical preparations used against diabetes. An intensive survey undertaken for over 4 years in the tribal belts of Orissa to identify the plants used in folk medicine and the methodologies employed by the herbal healers have revealed that 35 species are being employed to cure diabetes. Out of 313 patients (185 males and 128 females) interviewed, 211 (67.5%) use Gymnema sylvestre, Momordica charantia, Trigonella foenum-graecum, Tinospora cordifolia, Pterocarpus marsupium, Andrographis paniculata and Rauwolfia serpentina in one form or the other regularly. Multi-plant preparations gave 80% results whereas single plant administration gave less than 50% results. Polyherbal treatment is advocated as multi-pronged attack to achieve desired results.

ABSTRACT

Phospholipase A₂ catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and releases fatty acids and lysophospholipids. When the fatty acid is the arachidonic acid, a complementary metabolism leads to pro-inflammatory mediators such as prostaglandins, leukotrienes, thromboxanes and platelet activating factors. Thus, modulating pro-inflammatory lipid mediator production by inhibiting PLA₂ activity remains a potential target for the development of new drugs for the treatment of inflammatory diseases. Medicinal plants have been explored therapeutically in traditional medicines and are a valuable source for drug discovery. Molecular docking studies were carried out for anti-inflammatory compounds from various plant sources with human non-pancreatic secretory phospholipase A₂.

ABSTRACT

Purine Nucleoside Phosphorylase (PNP) is an enzyme that functions in the purine salvage pathway by catalyzing the phosphorolysis of purine nucleoside thereby releasing the purine bases and phosphorylated sugar. Interest in PNP as a drug target arises from its role in T-cell proliferation and T-cell mediated autoimmune diseases. Recently, we have isolated the compound, coixol, from the plant Scoparia dulcis L. and its three-dimensional structure has been elucidated. As the structure of the coixol mimics the structure of PNP inhibitors, we have undertaken the molecular modeling studies of this compound with Human PNP, as a screening test for further in vivo studies. Generally PNP inhibitors have been synthesized with a ribose sugar moiety attached to them. As the plant Scoparia dulcis L. contains glucose and rhamnose (six membered ring), these sugar moieties are also included along with ribose in the presen study. In order to understand the binding mode and interactions of the isolated compound attached with different sugar groups, we have carried out the docking studies with Human PNP. The interactions between the ligand molecules and active site residues of Human PNP are similar to those of solved complexes and the energy values are also comparable, which clearly indicate that these ligands may act as inhibitors.

1. Introduction

India is an exquisite example of biodiversity. From time immemorial scholars like Charaka, Barathwaj, Athreyan, Agnivesha, Dhanyandhari, Shushruthan, Wakbadan and Bharathduja etc. have studied and explored the possibility of such a diversity for human welfare and the most conspicuous exploration in this field has lead to the discovery of so many indigenous medicinal plants that were scripted mainly in Vedas (1500 BC) that contain rich materials on herbal lore of that time. Charaka in his medical treatise – Charaka samhita delt with innumerable medicinal plants, their usage and descriptions. Besides the two above said texts, treatise like Sushruth samhita and Ashtangha hridaya samhita are a few among other precious ancient texts. Thus the gradual accumulation of practical and systematic medical knowledge lead to the formation of Ayurveda.

Ayurveda - the science of life, prevention and longevity, is the oldest and most holistic or comprehensive medical system available. It was placed in written form over 2000 years ago. Ayurveda is said to have been first complied as a text by Agnivesha (Agniveshtantra). This text was later revised by Charaka and renamed as Charaka samhita (completed by Dhridhabala). The concept of medicines envisaged in the Ayurveda comes from the monumental scripture called Ashtanga hridaya and Sahasrayoga.

ABSTRACT

Free radical scavenging property and antimicrobial activity of Triphala, the herbal product made of equal proportion of Terminalia chebula, Terminalia bellerica and Emblica officinalis were evaluated. Ethanol extracts of the formulation were tested for its invitro antioxidant activity by using improved ABTS radical cation decolorizing assay and invitro antimicrobial activity against Streptococcus mutans (predominantly involved in bio-film formation) by both well-diffusion and turbidity method to determine its minimal inhibitory concentration. An effort was also made to correlate its antiplaque activity using an invitro assay (where conditions kept similar to oral cavity) with Triphala and two commercial toothpastes (Colgate and Pepsodent). The ethanol extract showed significant antimicrobial and antioxidant activity. Triphala showed effective antiplaque activity when compared with the other tooth pastes. The herbal extract effectively inhibited the bio-film formation and more antioxidant activity exhibited by the extract might protect the gum cells effectively from free radicals than the commercial toothpastes, having less antioxidant activity.

ABSTRACT

Traditionally, the rural communities of Trivandrum have been preserving small tracts of virgin forests or small groves since time immemorial due to strong religious beliefs anointed with the groves. These forest patches are biodiversity rich communities and a large number of medicinal plants of the region are confined to these groves only. The indigenous people use the plants for medicinal as well as religious purposes. Over the years, such forests have been attributed as a treasure trove of threatened species and dispensary of medicinal plants. During the investigation, 132 medicinal plants belonging to 63 families and 119 genera were recorded from 25 miniature sacred forests of Trivandrum district. This study was based on medicinal plants used in traditional health care by local and indigenous people. About 80% local people use different parts of plants for various ailments. The present finding revealed that most of the plants are used for skin diseases, cold, cough and fever, etc. Leaves are predominantly used than the other plant parts. Important families within the groves were Apocynaceae, Papilionaceae, Urticaceae, Combretaceae and Euphorbiaceae. Presence of a large number of monospecific families indicates immediate need of a conservation measure to secure sustainable utilization of medicinal plants of sacred groves of Trivandrum district.

1. Introduction

Phytochemists and pharmacologists from all over the world obtain several patents for herbal drugs developed on the basis of the indigenous knowledge systems of different ethnic groups. Many countries rich in traditional medicinal knowledge have been conducting ethnobotanical survey of medicinal plants (Patil and Bhaskar, 2006). In India the native people have exploited a variety of herbal medicines for effective curing of various diseases. The plants used, preparation and administration of drug varies from area to area (Rajaram, 2006). The ethnobotanical research still plays its evident scientific role in stimulating further phytochemical and pharmacological studies (Leporatti and Corradi, 2001).

The objective of this study is to interact with Kani traditional healers and document their traditional knowledge. The Kani-tribals are one of the most primitive hill tribes of Western Ghats. They occupy the slopes of the Agasthiarmalai in Tirunelveli, Tamil Nadu, India. Some ethnobotanical studies have been done in this area (Anonymous, 1948-76, Chopara et al., 1956, Satyavati, 1976, Jain, 1981, Nagendra Prasad et al., 1996). The impact of deforestation, urbanization and modernization makes the tribals shifting from their natural habitats to urban areas. Documenting the traditional knowledge through ethnobotanical studies is important for the conservation of medicinal plants and traditional medicines.

ABSTRACT

In vitro culture and cryopreservation techniques are complementary to ex situ conservation of plant genetic resources that are vegetatively propagated or having recalcitrant seeds. An in vitro germplasm conservation study has been conducted on Banana passionfruit (Passiflora mollissima), a plant used in rural medicine. Micropropagated plants survived up to 12 weeks in Murashige and Skoog medium supplemented with 0.5% sucrose. Cryopreservation experiments were carried out on the explants (apical and/or axillary buds), pre-treated with vitrification solutions, dimethyl sulphoxide (DMSO) and PVS2 (Plant vitrification solution-2) with or without desiccation treatment and frozen in liquid nitrogen (LN). The survival and regeneration of apical buds were higher than axillary buds when pre-treated with 15% DMSO. Treatment with 20 % PVS2 for 1 h followed by 100 % PVS2 for 20 min gave maximum shoot recovery (40%) from cryopreserved apical buds. Desiccation alone was not successful but combination of pre-treatment of PVS2 (20% for 1h and 100% for 20min) with 3 h desiccation gave 30% plant survival from frozen apical buds. Regenerated shoots were rooted on MS medium with 1.0 mgl-1 naphthalene acetic acid (NAA) and 0.2 % activated charcoal.

ABSTRACT

In this study, different concentrations of acetone, alcohol, benzene and chloroform extract of Wedelia chinensis were carried out against human pathogenic bacterial and fungal strains. High frequency of anti-bacterial activity was observed on the Escherichia coli. Moderate amount of antibacterial activity was observed on Staphylococcus aureus. Highly resistant bacterium to this plant was screened through antibacterial pour plate method, which showed on the following organisms Citrobacter diversens, Serratia marcescens and Streptococcus pyogenes. The aim of this study is main evaluation of antimicrobial activity of plant. The ethanolic extracts demonstrating antibacterial and antifungal activity could result in the discovery of antibacterial and antifungal agents.

ABSTRACT

Solanum trilobatum Linn. (Solanaceae) was investigated for the induction of tumor using a soil borne bacterium, Agrobacterium tumefaciens. Explants like stem segments and nodes were infected with bacterium. Tumors were induced from nodal explants within 10 days of infection. These explants were then transferred to MS basal medium and their growth was observed to be independent of exogenous supply of phytohormones. The growth rate of transformed callus was 10-fold as compared to control. They are used as a promising source of secondary metabolites of medicinal significance.

ABSTRACT

Albizzia lebbeck is commonly called as “vagai” in Tamil belonging to family Mimosaceae, is a large deciduous tree commonly seen throughout India. Our present aim, was to study the CNS depressant activity of Albizzia lebbeck in wistar albino rats. The bark and leaves of Albizzia lebbeck were shade dried and coarsely powdered. About 500 gm of powder was extracted using petroleum ether, chloroform and alcohol by cold maceration process. The depressant activity was accessed using actophotometer. It was claimed that in indigenous system of medicine the drug has anti-inflammatory, opthalmopathy, leucoderma, leprosy, wounds, ulcers and CNS depressant activity.

ABSTRACT

Our country is well known for developing into rich and ancient traditional system of medicines based on plants. For centuries, these ancient cultures and professional practicing on traditional system viz. Ayurveda, Unani and Siddha could remain in house hold and pass through generations to generation. India is being a developing country, of late uses bioactive natural products (secondary metabolites) using in vitro culture technology of the medicinal plant species. Recently, commercial utilization of these compounds using modern techniques is in good progress. The developed western affluent world has to now depend on these compounds mostly in drug and pharmaceutical industry in recent years. Orchids as medicinal herbs, details on plant medicinal potentialities, traditional medicinal systems viz. Ayurveda, Unani and Siddha have been described.

ABSTRACT

The present communication thr ows light on 118 species of flowering plants (Angiosperms) found in Dewghat forest (in Koraon forest range of Uttar Pradesh), which are traditionally used by the “Kol” tribal community and some other forest dwelling people, viz. Belbans, Bahelia, Mushahar etc. An enumeration of these plants materials is prepared with their botanical names, local / vernacular names, family names and using practices provided in this paper. This is the first ever report from the area under study on this aspect. In case of Anogeissus latifolia the leaf juice is used poisonous insect bite, grounded flower used in chronic diseases and its fruit is used asthma. The seeds of Cleome viscosa are given orally in stomach diseases. The leaves of Cassia alata used for ringworms and skin disease. The leaves juice and seed of Euphorbia thymifolia is used as a strong purgative. It leaf juice is also used for the remedy of ringworms and other skin diseases. It is an antidote for snakebite. The present study is aimed at indexing of all medicinal flora occurring in Dewghat forest (U.P.) and their uses for the benefit of the researchers to undertake studies on the prospects and potential of commercial exploitation.

ABSTRACT

A low molecular weight antifungal protein has been purified from the leaves of a medicinal herb, Mentha viridis, L. using a combination of cold acetone precipitation, carboxymethyl cellulose column, Q-sepharose column chromatography, and preparatory native PAGE techniques. The purified protein migrated as a single band on SDS-PAGE, with a molecular weight of ca 15.5 kDa. The purified leaf protein of M. viridis inhibited mycelial growth of phytopathogens such as Fusarium solani, Fusarium sp., and Pythium aphanidermatum. The purified protein from the leaves of M. viridis inhibited the growth of human pathogens such as Candida albicans and C. tropicalis (75 mg/mL). This inhibition is concentration dependent. The purified antifungal protein of leaves of M. viridis did not show protease, chitinase, and b-1, 3 glucanase activity.

ABSTRACT

The Kolanthamamalai hill an offshoot of Kalakadu Mundanthurai Tiger Reserve of Southern Western Ghats is one of the important forest reserves of Tirunelveli District. The physiography of this range differs much from the adjacent portions of Southern Western Ghats of Tamil Nadu state due to its discontinuity, varying altitude, ridges and their height and breadth. The altitude of the hilly rugged terrain ranges from 150 to 300m, with the highest peak being Kolanthamamalai. Field visits were made in the study area to survey and to gather information on medicinal flora. The vegetation of Kolanthamamalai hill is a mixed dry deciduous type characteristic of the high and low altitude of the mountains respectively. Lowland vegetation girdles the base of the mountain on the eastern and southern side of the hills. Along the foot hills vegetation is an open scrub with occasional trees. The low-lying areas and the seasonal watercourses are densely vegetated. The natural barriers, habitat diversity, climate and moderate rainfall have resulted in the development of unique plant diversity.

ABSTRACT

The present study was designed to evaluate the effect of α-mangostin on cardiac markers against isoproterenol-induced myocardial infarction in rats. Induction of rats with Isoproterenol (150 mg/kg body wt, i.p) for 2 days resulted in a marked reduction in the activities of cardiac tissue marker enzymes (LDH, CPK, GOT and GPT). Pretreatment with α-mangostin (200 mg/kg of body wt/day) orally for 6 days significantly prevented these alterations and restored the enzyme activities to near normal levels. In conclusion, these findings demonstrate the protective effect of α-mangostin on isoproterenol induced experimental myocardial infarction in rats.

ABSTRACT

Biodiversity is a major resource for drug development. Since ancient times mankind has exploited nature for all kind of useful products and enjoyed the colors, flavors and fragrances of flowers and leaves etc. The current global resurgence in the use of plant based drugs offers an ideal opportunity for the country to emerge as a major player in the herbal products market. Herbal medicines are chemically diverse and may contain thousands of bioactive compounds, which can be of immense value to the pharmaceutical industries. Since ages, herbal medicine has been a major part of traditional knowledge play an important role for identification of biological resources worthy of commercial exploitation. Origanum vulgare L. is a wild plant of Labiatae family grows widely in Ladakh region (11500 to 13000 ft). The local people of this region use this plant for various ailment and culinary purposes due to the potential of active ingredients. The present objective of this study is to isolate the thymol content, a major ingredient of O. vulgare and quantifying the same. The volatile oils were extracted through hydro-distillation and active compound was analyzed by Gas Chromatography (GC-17A Shimadzu). Our results demonstrate that the thymol content of O. vulgare from this region is 80.28% as compared to plants grown at other low altitude regions. It is well known that plants under stress respond with biosynthesis of phytochemicals to enable them to adapt to the harsh environmental conditions. Ladakh being unique in many aspects due to its geo-climatic conditions like high elevation, extreme cold, dry weather and low humidity. Plants surviving in Ladakh therefore have unique chemical composition as compared to those grown elsewhere.

ABSTRACT

Three medicinal plants namely, Eryngium foetidum (Apiaceae), Centella asiatic (Apiaceae) and Hemidesmus indicus (Asclepiadaceae) were collected from Similipa Biosphere Reserve forest and used in this investigation. Ethanolic, methanolic chloroform and aqueous extracts of different parts like leaf, root and stem were prepare through standard techniques and screened for their anticandidal potencies agains Candida albicans, C. tropicalis and C. krusei. Anticandidal activity of the extracts wer tested using preliminary and secondary screening methods, like disc diffusion metho (DDM) & agar-cup-plate-method (ACPM) and poisoned food technique (PFT respectively. On an average the inhibitory tendency of the extracts can be arranged as ethanolic extracts > methanolic extacts > chloroform extracts > aqueous extracts Maximum degree of inhibition was observed against C. krusei. Among the plant Hemidesmus indicus showed better anticandidal activities in comparison to other tw plants tested.

1. Introduction

The heritage of medicinal plants used in India has an ancient history. The epic of Ramayana witnessed Sanjivani Booti as life saving drug. Two important compendia on medicinal plants i.e., Charaka Samhita and Sushruta Samhita were published between 1000 BC and 600 BC. In these Ayurvedic literatures, uses of more than 1,200 plant drugs along with their action and specific therapeutic applications are mentioned. Charaka made 50 vargas (groups) of drugs while Sushruta made 38 ganas (groups) of drugs (Singh and Gautam, 1997). India, with its diverse agro-climate, harbors large plant-diversity. Eastern Himalayas comprising Darjeeling, Sikkim and adjacent Nepal as well as Bhutan hills are very rich in florastic diversity, several of which are endangered but economically useful medicinal plants with high demand in national as well as in international market (Manandhar, 1999). A great many of these wild Himalayan medicinal plants have found their way into modern bio-pharmaceuticals and bio-cosmetics in recent times (Rai et. al., 2000). One such species is Nepalese Kutki (Picrorhiza scrophulariiflora, Pennell) belongs to family Scrophulariaceae.

P. scrophulariiflora is better known by the name Nepali Kutki. The rhizome of this plant has been found to contain the chemicals like Kutkin, D-mannitol and vanillic acid (Yang, 1996). It is said to have effects on fever, malnutrition due to digestive disturbance, jaundice, diarrhoea and dysentery (Zhang et al., 1994). In India it is valued for their demonstrated value as an antibiotic. They are also used as an adulterant of, or as substitute for, Gentiana kurrooa. Properties that these two species share include the ability to stimulate appetite and gastric secretions (Jain, 1968). In Nepal rhizome of this species is also used as a cathartic, in case of dyspepsia, as a purgative as well as in the treatment of scorpion bites (Anon., 1993). Some of the important active photochemicals are listed in Table 1.

ABSTRACT

According to WHO report, over 80% of the world population relies on traditional medicine, largely plant based for their primary healthcare needs. India has 15 Agroclimatic zones, 12 ecoregions and 47,000-plant sp. and is one of the 12-mega biodiversity centres. The Indian Systems of Medicine have identified 1500 medicinal plants, of which 500 species are used in the preparation of commercial drugs. These medicinal plants contribute to 80% of the raw materials used in the preparation of drugs.

ABSTRACT

Advances in biotechnology, particularly methods for culturing plant cells and tissues, can provide new means for the commercial processing of even rare plants and the chemicals they produce. These new technologies will extend and enhance the usefulness of plants as renewable resources of valuable chemicals. Aristolochic acid is mainly found in the genus Aristolochia. Aristolochic acid has been shown to have anti-tumour activity and has been recommended for its activity against various effects of snakebite and its capacity to inhibit phospholipase A₂ or other enzymes. Considering the importance of medicinal use of A. bracteolata and A. indica, in the present study, they were selected for in vitro development and rapid multiplication of plant through tissue culture. The study was undertaken to enhance the in vitro production of Aristolochic acid in different tissue cultures. Cell suspension culture was established from friable calli of both the species in MS liquid medium. Bergmann plating technique was carried out for cell line selection for more production of Aristolochic acid. Primary transformation was carried out by Agrobacterium tumefaciens strain LBA4404 / pTOK233 with Gus and hpt genes to confirm the transgenic potential of A. bracteolata and A. indica. In order to enhance the production of aristolochic acid in in vitro culture condition, the physical, chemical and biological conditions were altered. Aristolochic acid was isolated, identified and purified from naturally growing plants as well as from tissue culture plants and all other culture staes

ABSTRACT

Advances in biotechnology, particularly methods for culturing plant cells and tissues can provide new means for the commercial processing of even rare plants and th chemicals they produce. These new technologies will extend and enhance the usefulness of plants as renewable resources of valuable chemicals. Aristolochic acid is mainly found in the genus Aristolochia. Aristolochic acid has been shown to hav anti-tumour activity and has been recommended for its activity against various effect of snakebite and its capacity to inhibit phospholipase A2 or other enzymes Considering the importance of medicinal use of A. bracteolata and A. indica, in th present study, they were selected for in vitro development and rapid multiplication o plant through tissue culture. The study was undertaken to enhance the in vitr production of Aristolochic acid in different tissue cultures. Cell suspension culture was established from friable calli of both the species in M liquid medium. Bergmann plating technique was carried out for cell line selection fo more production of Aristolochic acid. Primary transformation was carried out by Agrobacterium tumefaciens strain LBA4404 / pTOK233 with Gus and hpt genes to confirm the transgenic potential of A. bracteolata and A. indica. In order to enhance the productio of aristolochic acid in in vitro culture condition, the physical, chemical and biologica conditions were altered. Aristolochic acid was isolated, identified and purified from naturally growing plants as well as from tissue culture plants and all other cultur stages.

ABSTRACT

A rapid micropropagation technique have been established for Solanum trilobatum L., a medicinal plant from internodal explant. Callus formation was observed from both cut ends of explants cultured on MS medium supplemented with 2,4-D (3.0 mg/l). Direct shoot and multiple shoot formation were noticed from explants (proximal cut end) on BAP (2.0 mg/l) alone and BAP (2.0 mg/l) + NAA (0.5 mg/l). Root formation was directly produced from the basal cut end of explants cultured on IBA (1.0 mg/l). In vitro plantlet regeneration has been achieved on BAP (2.0 mg/l) + IBA (1.0 mg/l). Regenerated plants were transferred to sterile vermiculite and soil mixture (3:1). In vitro propagated plants were grew successfully under the field conditions. In vitro propagation can be a useful tool for the conservation of this medicinal species.

ABSTRACT

An efficient and reliable micropropagation and mycorrhization techniques were established for a medicinal plant Solanum trilobatum L., using internodal explant. Callus formation occurred from both the cut ends of explants cultured on MS medium supplemented with 2,4-D (3.0 mg/l). Direct shoot and multiple shoot formation were noticed from the proximal cut end of explants on BA (2.0 mg/l) alone and BA (2.0 mg/ l) + NAA (0.5 mg/l). Root formation was directly induced from the basal cut end of explants cultured on IBA (1.0 mg/l). In vitro plantlet regeneration has been achieved on BA (2.0 mg/l) + IBA (1.0 mg/l). After 4 weeks, well-developed in vitro plantlets were hardened in plastic cups filled with sterile soil and vermiculite (3:1). During acclimatization, sterile Arbuscular Mycorrhizal (AM) fungal spores were added for enhance the survivability of in vitro plants in the field condition. The level of biochemical changes and increased survivability were studied in 10 weeks old plants inoculated with spores of Glomus mosseae and Gigaspora margarita.

Introduction

Solanum trilobatum L., an important medicinal plant, belonging to Solanaceae is widely distributed in South Asia and has been used in Indian traditional medicinal systems of Siddha and Ayurveda. The members of Solanaceae accumulate steroidal alkaloids based on a C₂₇ cholestane skeleton, e.g. solasodine and tomatidine. Recently, mixtures of solasodine glycosides have been successfully used for the treatment of certain human skin carcinomas (Cham, 1994). Solasodine glycosides have antineoplastic activity in cell culture, animals and in humans (Cham and Daunter1990). It has been demonstrated that specific endogenous lectins which are present on the plasma membranes of susceptible cells recognize and bind the sugar moiety of the solasodine glycosides and the glycosides are subsequently internalized and cause cell death (Cham and Daunter1990). Hence the present study was undertaken to isolate the solasodine from S. trilobatum.