eChapters

Inverse Laplace Transform of 1/(s - 2)2 is
(A) exp(2t)            (B) t exp(2t)
(C) 2t exp(2t)         (D) t2 exp(2t)

For station X, the maximum one day rainfall with 25 years return period is 100 mm. The probability of a on day rainfall equal to or greater than 100 mm at station X occurring at least once in 15 successive years is
(A) 0.458             (B) 0.500
(C) 0.637            (D) 0.990

The curl of the vector A= xyi + yzj + zxk (i, j and k represents unit vectors along the three orthogonal axis) is

(A) xi + yj + zk         (B) – xi – yj – zk

(C) yi + zj + xk          (D) – yi - zj - xk

Integrating the function f (x) = 1 + e−x sin (4x) over the interval [0, 1]

using Simpson’s 1/3rd rule gives

(A) 1.021         (B) 0.091

(C) 1.321         (D) 2.642

Eigen values of the matrix are

(A) 1 and 2             (B) 1 and 3

(C) 1 and 4            (D) 2 and 3

The researcher desires to make a study on the socio-cultural aspects of cyber spatial interactions. As you are aware, cyber space or Internet has emerged as a powerful medium of communication. The survey is meant purely for academic purpose and therefore, shall not be passed on to any one and the data obtained from the respondents shall be kept confidential for all practical purposes. The researcher sincerely requests your honest responses. The questions are of people in general, and that there is no right or wrong answers.

The Internet, new information and communication technologies today are being entrenched into the fabric of our society at a very rapid rate. The Internet has emerged as an extraordinary prominent medium having a tremendous impact on and within society.

This chapter reports the analysis of the data collected from Phase One and Phase Two of the research .The chapter is organized in three sections. The first section presents the analysis of the data collected in the first phase through the administration of the survey questionnaire (Appendix-I). It deals with a study of the potential of Internet as a communication tool through an account of the Internet usage habits of the two cities followed by a presentation of their perception, attitudes and internet adoption among the people.

This chapter outlines the design of Phase One and Phase Two of the research. It outlines the research approaches and methodology utilized in Phase One and Phase Two of the research. The chapter also provides details of the sampling strategies, data sources, collection and analysis of both the phases; and finally addresses ethical issues.

The aim of the research is to make a study of the role of Internet as a medium of interaction, its impact on the society from the social and cultural point of view. Keeping these objectives in mind, the researcher has referred to contemporary researches, books and articles available in the area. The researcher admits that the number of studies available in the area is very much dispersed. In the line of the research questions formulated for conducting the study, the researcher has explored the relevant studies undertaken so far by different researchers.

This research seeks to address some major concerns of communication through cyberspace and its socio-cultural implications. In particular, it seeks to address the use of cyberspace or Internet as a potential medium of communication for the people of Odisha and the relatively positive and negative outcomes of the use of this medium. It investigates the current Internet-mediated communication practice in the twin city (Bhubaneswar and Cuttack) of Odisha and explores the social and cultural implications of the new communication mode.

Example: Isolation and Characterization of Protease Inhibitor Gene and Promoter from Black gram (Vigna mungo L.) and Genetic Transformation and Molecular Analysis of Transgenic Tobacco

The genetic information which makes up the genes of higher plants is stored in DNA sequences of the nuclear chromosomes and the organelle genome. There is such an enormous amount of DNA in higher plant cell, that no two organisms are likely to be identical in DNA base sequence. 

Developments in recombinant DNA technology have made it possible to transfer gene from any source across species barrier. In fact, transfer and expression of foreign genes in plant cells is now a routine procedure. Of the several methods tried so far, the system based on Ti plasmid of Aprobacterium tumefaciens appears to be most successful for transformation of plant cells.

Protoplasts are cells which have had their cell wall removed, usually by digestion with enzymes. Cellulase enzymes digest the cellulose in plant cell walls while pectinase enzymes break down the pectin holding cells together. Once the cell wall has been removed the resulting protoplast is spherical in shape. Digestion is usually carried out after incubation in an osmoticum (a solution of higher concentration than the cell contents which causes the cells to plasmolyse). This makes the cell walls easier to digest. Debris is filtered and/or centrifuged out of the suspension and the protoplasts are then centrifuged to form a pellet. On resuspension the protoplasts can be cultured on media which induce cell division and differentiation.

DNA can be sequenced in both double stranded and single stranded templates by cloning in plasmid or phage vectors. There are two sequencing methods.

M13 is a single stranded filamentous DNA phage. Vectors have been developed from M13 phase by Joachim Messing (1983), which are suitable for preparation of single stranded DNA required for sequencing by Sanger’s method. These are derivatives of M13 wild type (size 6.407 kb).

The polymerase chain reaction (PCR) is a very powerful new method used for in vitro to amplify a deoxynibonuclic specific region that lies between two regions of known DNA sequence single molecule to million folds in short time. This is achieved by an enzyme taq. DNA polymerase which is thermostable using this enzyme can synthesize microgram quantities of DNA of specified length and sequence from pictogram quantities of complex template in a few hours time.

Complementary DNA (cDNA) is a double stranded DNA which is an exact copy of the mRNA, i.e. it consists of group of molecules possessing the information contained in various mRNAs but with termini equivalent to or related to those produced by restriction endonucleases. The use of cDNA is an absolute prerequisite for the expression of eukaryotic proteins in bacteria.

Recombinant DNA is advancement in molecular technology which allows manipulation of genomes of higher organisms and dissection of their complex regulatory circuits. This new methodology is being used to understand the biochemistry of living organisms. The isolation of gene begins with the cloning of fragmented DNA of the organism into appropriate vector. 

Exchange of genetic material rarely occurs between unreleated organisms and even similar species may not exchange chromosomal genes. A certain degree of DNA sequence homology is a prerequisite for genetic recombination in vivo. However, recombination between DNA molecules in vitro is not subject to such taxonomic restrictions.

Nucleic acid hybridization requires denaturation i.e. the breakage of H-bridges between the complementary strands of the DNA either by heating at high temperatures or by treatment with denaturing chemicals like formamide. The restricted DNA, therefore, is denatured before transfer from the gel onto synthetic membrane since only single stranded DNA binds to nitrocellulose.

DNA fragment to be used as a probe should be purified by gel electrophoresis. The band can be located after staining with ethidium bromide and electroeluted.

The in vitro radioactive labeling of DNA fragment is accomplished by the incorporation of a radioactive nucleotide during strand synthesis with Klenow enzyme (large subunit of DNA polymerase-I).

Polysomes consist of two or more ribosome traversing a strand of mRNA, translating the nucleotide sequence into the corresponding amino acid sequence. Usually at any time, many ribosomes are translating an mRNA simultaneously forming a structure called a polyribosome or polysomes. One reason for isolating polyribosomes is to resolve them on sucrose gradients to determine the extent of ribosome loading. The second main reason is to provide a source of mRNA for in vitro translation.

RNA is transcriptional product of DNA. The total RNA includes three classes, rRNA, tRNA and mRNA. The ribonucleic acids exists as ribonucleo protein particles in intact cells. The ribonucleo protein complex is dissociated by SDS in to RNA and protein deproutinised by phenol and the free RNA left in the aqueous solution is precipitated in cold by adding alcohol. 

There are various methods available for isolation of high molecular weight genomic DNA from plants. The basic steps involved in all these methods are removal of cell wall, nuclear membrane, proteins, lipids, carbohydrates, cell debris, RNA without affecting the integrity of DNA. The two major factors that affect DNA integrity are action of endogenous nucleases and impact of mechanical shearing.

Enterobacteria phage ? is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E.coli). It was discovered by Esther Lederberg in 1950. The wild type of this virus has a temperate life cycle that allows it to either reside within the genome of its host through lysogeny or enter into a lytic phase, during which it kills and lyses the cell to produce offspring. Lambda strains, mutated at specific sites, are unable to lysogenize cells; instead, they grow and enter the lytic cycle after superinfecting an already lysogenized cell.

This is a procedure for transforming bacterial cells with circular DNA plasmids containing an origin of autonomous DNA replication and a selective marker (antibiotic resistance). Logarithmically growing cells are made permeable to DNA by incubation in CaCl2 and the DNA is taken up from the surrounding buffer.

Plasmid is a double stranded, self replicating, circular extra chromosomal DNA of bacterium. It is used in recombinant DNA experiments to clone genes from other organisms and make large quantities of their DNA. Plasmid can be transferred between same species or between different species. The sizes of plasmids range from 1-1000 kbps. Plasmids are part of mobilomes (total of all mobile genetic elements in a genome) like transposons or prophages and are associated with conjugation.

Plant biotechnology can be defined as the introduction of desirable traits into plants through plant tissue culture and genetic engineering techniques. The recombinant DNA technology is used to modify the expression of genes already present in the plants or to introduce new genes of other species with which the plant cannot be bred conventionally.

Unique Achievement in Plant Breeding

Rice

TN-1: First dwarf variety of rice developed in world

IR-8: Second dwarf and first high yielding variety of rice developed in world

Jaya: First dwarf variety of rice developed in India

Agricultural University

17.1 Central Agricultural Universities

1. Central Agricultural University, Imphal Manipur

2. Rani Laxmi Bai Central Agricultural University, Jhansi Uttar Pradesh

3. Dr. Rajendra Prasad Central Agricultural University, Pusa (Samastipur)

Agricultural Research Centers in World

1. Consultative Group on International Agricultural Research (CGIAR Centers)- Montpellier, France (1971)

2. International Center For Tropical Agriculture (CIAT)-Colombia (1969)

All India Coordinated Research Projects

1. AICRP on Nematodes, New Delhi

2. AICRP on Maize, New Delhi

3. AICRP Rice, Hyderabad

4. AICRP on Chickpea, Kanpur

Directorates/Project Directorates

1. ICAR-Directorate of Groundnut Research, Junagarh, Gujarat

2. ICAR-Directorate of Soybean Research, Indore, Madhya Pradesh

1. ICAR-National Bureau of Plant Genetics Resources, New Delhi

2. ICAR-National Bureau of Agriculturally Important Micro-organisms, Mau, Uttar Pradesh

1. ICAR-National Research Centre for Banana, Trichi

2. ICAR-National Research Centre for Grapes, Pune

3. ICAR-National Research Centre for Litchi, Muzaffarpur

1. ICAR-Central Island Agricultural Research Institute, Port Blair

2. ICAR-Central Arid Zone Research Institute, Jodhpur

3. ICAR-Central Avian Research Institute, Izatnagar

Intellectual property : Refers to creations of the mind: inventions, literary and artistic works, and symbols, names, images, and designs.

Biometrical genetics: This is a branches of genetics which uses the statistical concept and procedures for the study of genetic principles.

1. Nucleus seed: It is the handful of original seed obtained from selected individual plants of a particular variety. It has the highest genetic purity and physical purity.

1. Ideotype : It is a biological model which is expected to perform in a predicted manner in a defined environment.

2. Shuttle breeding: Growing of segregating generation in different environments.

1. Agricultural biotechnology: Agricultural biotechnology is a collection of scientific techniques used to improve plants, animals and microorganisms.

2. Gel electrophoresis: Analytical technique by which usually large biomolecules (proteins, DNA) are separated through a gel by application of an electric field.

A. Define/Explain the following

1. Heteroploidy: Any change in chromosome number from the diploid state.

2. Euploidy: Numerical change in the entire genome.

A. Define/Explain the followings

1. Mutation: Sudden heritable change in the phenotype of an individual.

2. Macro-mutations: Mutations with distinct morphological changes in the phenotype are called macro-mutations. Such mutations are found in qualitative character and, therefore, are also called oligogenic mutations.

2.1 Introduction in Plant Breeding

A. Define/Explain the followings

1. Plant breeding: Plant breeding is defined as the art, science and technology of improving the genetic make-up of crop plants in relation to their economic use for mankind.

A. Define / Explain the following

1. Mitosis: The multiplication of a body cell into two daughter cells of equal size and containing the same number of chromosomes as in the parent cell is called mitosis or somatic division.

2. Meiosis: Meiosis occurs only in gonads (in germ mother cells) during the formation of gametes like sperm and ovum. Meiosis is a process by means of which double number or 2N or diploid chromosomes is reduced to its half number or n or haploid. It is also called reduction process.

Sonograms of animals suffering from different diseases are presented along with other investigations as case studies to provide a comprehensive picture of the disease.

Utility of ultrasound has extended from diagnostic realm to alternate therapies. Therapeutic ultrasound is also known as ultrasound physiotherapy. It is a commonly used modality for the rehabilitation of the soft tissues of the musculoskeletal system.