eChapters

Veterinary epidemiology is the study of disease within a livestock population, dealing with the investigation (including identification, quantification, and examination), of the direct and indirect determinants of disease distribution in animal populations. Any epidemiological investigation starts with whether there is an animal health problem in a population, which requires data collection on animal, management, economic, and environmental factors. These data are then analyzed descriptively to assess the frequency of disease occurrence. The results are then used to decide whether investigations of causal relationships between disease occurrence and potential risk factors are necessary for the development of control or prevention policies. Once the relationships are understood, necessary interventions for the disease are then planned and their impacts assessed. Hence, the goal of epidemiological investigations is the control and prevention of animal disease problems by integrating the available pieces of evidence into the decision-making process for disease control policy development.

Learning objectives

1. Recognise inflammatory cell types

2. Recognise common skin pathogens

3. Diagnostic test are best for each case

4. Gain experience in interpretation

5. Recognise important artefacts

″The most crucial step towards healing is having the right diagnosis″. While approaching a case, a clinician should follow appropriate steps in a systematic way for accurate diagnosis of dermatological disorders. A careful history is a first step to choose appropriate diagnostic tests.

Fruits represent a vital component of global agriculture, serving as not only a significant source of essential nutrients but also a lucrative sector of the economy. However, from the moment of their inception on the tree to their arrival on consumers’ tables, fruits undergo a complex journey influenced by a myriad of factors. Among these, pre-harvest practices wield a profound impact on fruit quality, enzymatic activities, texture, ripening, and ultimately, post-harvest transportation losses. Each pre-harvest practice, from mineral nutrition to harvesting methods, plays a distinct role in shaping the biochemical composition and physical attributes of fruits, ultimately affecting their marketability and shelf life. Mineral nutrition stands as one of the foundational pillars of fruit production, with essential minerals serving as building blocks for various biochemical processes. Irrigation practices represent another critical aspect of pre-harvest management, as water availability profoundly impacts fruit development and composition. Different irrigation regimes can influence enzymatic activities, fruit texture, and the timing of ripening, necessitating strategic water management to achieve desired fruit quality while minimizing post-harvest losses. Canopy regulation, achieved through training and pruning techniques, offers growers a means to manipulate light penetration and airflow within the orchard. Moreover, the application of plant growth regulators (PGRs) emerges as a tool for fine-tuning fruit development and ripening processes. By modulating hormonal signals, PGRs can alter enzymatic activities, influence fruit texture, and mitigate transportation losses. However, judicious application is paramount to avoid adverse effects on fruit quality and safety. Harvesting decisions, including the determination of optimal maturity and the timing and method of harvest, profoundly impact fruit quality and post-harvest losses.

1. Introduction

The National Agroforestry Policy of India is a comprehensive policy framework designed to improve agricultural livelihoods by maximizing agricultural productivity including animal productivity along with tree cover and mitigating climate change. The Government of India launched the policy in February 2014 during the World Congress on Agroforestry, held in Delhi. The focus of the policy is to address Agroforestry, which is an integrated system of practicing agriculture, livestock farming and forest activities on the same unit of land, storing high carbon, there by mitigating climate change. Agroforestry systems have the potential to be more beneficial than traditional agriculture and forest production practices. They may be able to give enhanced productivity, as well as social, economic, and environmental advantages, as well as a broader variety of ecological products and services. It’s important to remember that these advantages are contingent on appropriate farm management. In 2014 India became the first nation in the world to adopt an inter-sectoral policy on agroforestry, which seeks to identify the bottlenecks in expansion on agroforestry in the country and pathways to remove the constraints in a systematic manner.

Introduction

The realm of agricultural policies and environmental policies stands as two critical pillars influencing the sustainability and equilibrium of our ecosystem. Agricultural policies primarily focus on regulations and measures instituted by governments or institutions to manage agricultural practices, production, distribution, and rural development. Conversely, environmental policies aim to safeguard and conserve natural resources, biodiversity, and ecosystems while mitigating the adverse impacts of human activities on the environment.

Overview of Agricultural and Environmental Policies

Agricultural policies often encompass subsidies, price controls, land use regulations, and trade agreements aimed at bolstering agricultural productivity, ensuring food security, and supporting rural economies (Fischer & Edmeades, 2010). Conversely, environmental policies encompass a broad spectrum of regulations, such as pollution control measures, wildlife protection, habitat conservation, and climate change mitigation strategies (Boyd & Banzhaf, 2007).

Importance of Understanding Their Interactions

Understanding the interactions between agricultural and environmental policies is crucial due to their interconnectedness and potential trade-offs. Agricultural activities significantly impact the environment through deforestation, water pollution from agricultural runoff, greenhouse gas emissions, and loss of biodiversity (Pretty et al., 2008). Conversely, environmental conditions greatly influence agricultural productivity and sustainability, including soil quality, water availability, and climate patterns. Recognition of these interdependencies is vital for devising coherent policies that foster sustainable agricultural practices without compromising environmental integrity (Tilman et al., 2011). The challenge lies in formulating policies that strike a balance between agricultural production goals and environmental conservation imperatives.

Statistics as a wide subject is used in almost all disciplines especially in research studies. Statistics provides scientific tools for representative data collection, appropriate data analysis and summarization of data and inferential procedure for drawing valid conclusion in the face of uncertainty. Each and every researcher should have some knowledge in Statistics and must use statistical tools in his or her research, one should know about the importance of statistical tools and how to use them in their research or survey. The quality assurance of the work must be dealt with: the statistical operations necessary to control and verify the analytical procedures as well as the resulting data making mistakes in analytical work is unavoidable. The emergence of statistical software in the twenty-first century has helped different researchers in the physical and social science to improve in the quality of research. Most renowned researchers in adopting this software in their data analysis have been able to identify the immense contribution to research findings (Adetola, 2013). Any quantitative research cannot be done effectively without Statistical Software. Moreover, it enables research data for easy presentation. It helps professionals to interact with data thereby paving way for creativity and innovation. Some are user friendly interface with drop-down tips for beginners (ATS, Ucla Edu, 2014). Advances in technology have improved all our lives (Akindutire, 2013) and has allowed experts greater freedom to come out with results within a twinkle of an eye than ever before where it takes time to finish analysis. This same technology has offer tremendous opportunity to research and keep research as a more interesting field of study. This study is significant, because it is an attempt to measure the direct impact of different statistical software on research analysis.

9.1. Introduction

The human population of the world will reach nearly 10 billion by 2050, boosting agricultural order-in a situation of humble financial development (FAO, 2017). At present, approximately 37.7% of total land surface is being utilized for crop production. From employment generation to contribution to National Income, agriculture sector is contributing a significant role in economic prosperity of the developed nations and is playing an active part in the economy of the developing countries as well. The growth of agriculture has resulted in a significant increase in the per-capita income of the rural community. Thus, a greater emphasis on agricultural sector is the need of the hour. For countries, like India, the agricultural sector accounts for 18% of GDP and provides employment to 50% of the country’s workforce. Development in the agricultural sector will enrich the rural development which will lead toward rural transformation and ultimately the desired structural transformation (Mogili and Deepak, 2018; Shah et al., 2019). The role of Artificial Intelligence (AI) has been obvious in the agricultural sector recently. The sector needs the impetus from AI based technology to maximize its yield including improper soil treatment, disease and pest infestation, big data requirements, low output, and knowledge gap between farmers and technology.

In some developing countries, milk from horses (Equus caballus) and donkeys (Equus asinus) is an essential food for poor farmers. Generally, horses are more commonly used for dairy purposes in cool areas and donkeys in dry semi-arid regions. Milking equines is time-consuming and has to be repeated five or six times a day. In addition, an equine will not release milk unless it is stimulated by the presence of its foal. Mare’s milk is commonly consumed in the steppe areas of Central Asia, where a traditional lactic-alcoholic beverage called koumiss is produced through fermentation. Horse milk is also an important source of animal protein for pastoralists in Mongolia. The consumption of donkey milk has become very marginal. In some African communities, donkey milk is consumed for medical purposes.

Equus is a genus of mammals in the family Equidae, which includes horses, asses, and zebras. Within the Equidae, Equus is the only recognized existing genus, comprising seven living species. Like Equidae more broadly, Equus has numerous extinct species recognized only from fossils. Equines are odd-toed ungulates with slender legs, long heads, relatively long necks, manes (erect in most subspecies), and long tails. All species are herbivorous, and mostly grazers, with simpler digestive systems than ruminants but able to subsist on lower-quality vegetation. The chromosome number of a horse is 64 (2n).

20th Livestock Census of India

India has highest cattle population (192.49 million), buffalo population (109.85), in the world producing 24% is the highest milk yield (221.1 million tons in the year 2021-2022, and per capita availability was 444 gm/day) in the world (Source NDDB). India produces 43% of world buffalo meat production. Unfortunately, 80% cattle and 70% buffaloes are non-descriptive and yield very low amount of milk.

After independence Indian Council of Agricultural Research (ICAR) has developed serval projects and plans for research and characterization of livestock species all over the country. Our country holds enormous genetic biodiversity in our domestic animals, result in increasing number of registered breeds in every decade. Breed registration Committee on its online meeting held on 5th December, 2023 approved registration of eight new breeds of livestock. This includes Aravali Chicken (Gujarat) Andamani Duck (Andaman and Nicobar Islands), Anjori Goat (Chhattisgarh), Andamani Goat (Andaman and Nicobar Islands), Bhimthadi Horse (Maharashtra), Andamani Pig (Andaman and Nicobar Islands), Macherla Sheep (Andhra Pradesh), Frieswal Cattle (Uttar Pradesh and Uttarakhand). Endangered, critical and vulnerable breeds of livestock also have been identified by ICAR which need more scientific research and multiplication breed.

The terms such as organic, natural, biological, biodynamic, altemative and sustainable farming have entered the agriculture vocabulary. Inspite of these differences in terminology, basically identical ideologies, ideas and technologies are reflected. The common objectives of all the system are to develop farming methods to restore, maintain or build-up soil productivity, to protect the environment instead of causing pollution, produce safe and healthy food, and preserve or help the small farmer.

In Japan, the farmers study the nature, learn from it and apply the knowledge on their farms. For instances growing plots should also be mulched with straw or plant residues, this imitates the conditions in natural forest where ground is never bare. Instead of using pesticides, strong herbs or plants can be grown around vegetable plots to ward off the insects. To keep the soil health natural, organic fertilizers are used. Soil microorganisms and earthworms take care of the processes involved in decomposition and mineralization of the organic substances, fix atmospheric nitrogen, solubilise insoluble forms of phosphate and produce natural growth promoting substances and antibiotics,

The concept of sustainable agriculture is gaining acceptance and momentum because of rapid degradation of natural resources, increase in the cost of production in conventional agriculture system and deterioration in the quality of rural life caused by environmental pollution. Moreover it is risky and uneconomical to practise conventional agriculture in over 70 percent of the cultivated area in India which is presently under rainfed farming. Much of the know how to practice organic farming models has rested on the individual farmers till now. They however do not have the benefit of appropriate technology and scientific information and also institutional support to ensure success of the new farming system.

Introduction

In the annals of history, the term “Renaissance” evokes images of a time marked by profound cultural, artistic, and scientific rebirth. The Renaissance of the 14th to the 17th century was characterized by an extraordinary surge in creativity and intellectual achievement, which laid the groundwork for the modern world. Today, we find ourselves at the cusp of a new Renaissance—a Digital Renaissance—propelled by the rapid and relentless advancements in technology. This new era, like its historical counterpart, is marked by a transformative shift that is redefining every aspect of human life, from how we work and communicate to how we learn and govern.

This chapter delves into the essence of this transformative era, exploring the fundamental technological innovations reshaping our world and their far-reaching impacts on society. We will examine the pivotal technologies driving the Digital Renaissance, including artificial intelligence, blockchain, the Internet of Things (IoT), and other emerging technologies such as virtual and augmented reality and 5G networks. By understanding these technologies and their applications, we can appreciate how they collectively contribute to a digital transformation that is as revolutionary as the artistic and scientific achievements of the past.

The digital age has revolutionized the way we live, work, and interact with the world around us. Digital transformation has become a crucial imperative for businesses of all sizes and industries, enabling them to not only adapt to the changing landscape but also thrive in it. (De Lucas,2021). The rapid advancement of technologies such as 5G, cloud computing, big data, artificial intelligence, IoT, and blockchain has opened up a plethora of opportunities for enterprises to enhance their operations, improve customer experiences, and gain a competitive edge. (Anjali et al, 2023). Businesses can increase their reach, automate jobs, obtain insights from data, personalize services, and streamline procedures by utilizing these technologies. They must reconsider their business models and implement more flexible and agile strategies in response to these exponential changes. (Bansal et al, 2020, Fenech et al, 2019).

During processing seeds are cleaned, dried, and graded which are then treated with specific fungicide and insecticide. Seed at this stage is at best quality, which are then packaged into various packaging materials in a specific weight. Packaging or bagging of processed and treated seed is a requisite for easy handling of the bulk seed. Seed packaging can be defined as filling with seed, weighing and sealing the container whereas seed package as storage container where the seeds are kept for marketing or shipping or till the next sowing time.

The Packaging of Seed Involves

1. Filling bags/ containers of uniform size with seeds of known weight.

2. Providing leaflets inside the package with information about any specific cultural practices needed.

3. Attaching labels or certification tags having information regarding all the quality parameters.

4. Temper-proof sealing of the bag.

5. Storage or transportation of the bags.

Concept: A few of the numerous environmental elements that affect seed quality maintenance are moisture, temperature, humidity, and storage conditions. When some seedborne diseases or pests like insects are taken into consideration, seed quality can still be negatively impacted. Applying one or more pesticides to seed is the most economical and efficient way to keep pests away and improve seed quality.

Because pesticides are harmful, handling treated seeds after application requires additional caution and safety measures.

Definition of Treated Seed

The definition of “treated” is “application of a pesticide or subject seed to a process intended to reduce, control, or repel insects, disease organisms, or other pests which attack the seed or seedlings.”

Introduction

The recognition of environment as the desired precondition for human existence and development both by Millennium Development Goals (MDG) as well as Sustainable Developmental Goals (SDG) unquestionably shows its importance and necessity. The impact of climate change is fast getting into our understanding and are realizing the enormity of the situation. Consequently, global environmental issues are a growing concern among the people and communities of various regions today. The impending ecological disasters have forced the right-thinking people and organizations to seek an alternative in the form of managing natural resources without jeopardizing the developmental activities. The challenge is to enhance the standard of living compatible with ecology and environment. This underlines rather unquestionably the need to pursue a line of lifestyle and development design that is compatible with nature and the issue of the environment cannot left to languish at the bottom of priorities.

Introduction

One of the main challenges in sustainable farming is determining the patentability of living organisms, including genetically modified crops and biotech innovations. Balancing the rights of inventors and the public’s access to agricultural resources can be so complex the Seed saving and farmer’s rights Seed saving is a traditional practice where farmers save and reuse seeds from their harvest. However, some intellectual property rights (IPR) regimes restrict farmers’ ability to save and exchange seeds, potentially limiting their autonomy and leading to dependence on commercial seed suppliers. Biopiracy and traditional knowledge Protecting traditional knowledge and preventing biopiracy is a significant concern in sustainable farming. Nearby agriculturists and inborn individuals habitually have imperative conventional information approximately plant sorts and rural procedures that’s esteemed, and they are decently compensated for their endeavors. Maintainable advancement became a major worldwide agenda thing within the afterward decades of the 20th century! According to the Brundtland report from 1987: “Our Common Future,” provided the most recognized concept of sustainable development, meeting future demands without sacrificing existing practices to be adopted for future aspects. (Ahmed Abdel-Latif, 2021). While a few Intellectual property rights have been pointed out such as the plant breeder’s rights trademark, security of plant assortments and ranchers’ rights (PPV and FR), topographical signs, and exchange insider facts are exceptionally significant to horticulture. In expansion, format plans for chips particularly planned for farming; logical papers, and tv programs covering thoughts related to horticulture and partnered segments must be considered. (Datta, 2016).

Introduction

For upliftment of the Indian economy agriculture plays an important role as it has contributed immensely. Nearly, three quarters of India’s family depend on rural incomes. Majority of India’s poor live in rural areas. Most people depend upon the crops, vegetables, fruits grown by these rural population. India’s food security is depended upon the inputs made by the farmers which has been a reason for increasing food insecurity in India. To meet with this accelerated pace a productive, competitive, diversified and sustainable agriculture sector needs to be introduced (The World Bank, 2012).

India being a global agricultural powerhouse, is the world’s largest producer of millet, pulses and spices and also has the largest cattle herd in the world, India also produces largest amount of wheat, rice and cotton. Agriculture is a part of India’s culture and tradition. In India, the culture, tradition and festivals are in a way related to different crops grown by the farmers. Along with this agriculture is deeply rooted with the community lifestyle in India. The community freely share their knowledge related to growing, sowing and harvesting of crops, they also distribute or share the produced crops among each other freely (OECD, 2018).

Introduction

Among the various countries worldwide, such as China, Kenya, and Sri Lanka, India stands as a significant player. In 2016, China and India collectively accounted for over 60% of the world’s total tea production. Furthermore, India holds the distinction of being the foremost producer of black tea, while China and Japan lead in green tea production. In India, Assam and Darjeeling are recognized as the primary states for tea production, while the Nilgiris and North-East regions also contribute significantly, playing a crucial role in boosting the country’s economic growth.

Assam annually produces over 400 million kilograms of tea, making it the largest tea-growing region globally.Assam has the world’s biggest tea plantations, which are important for India’s economy. The state is near the Brahmaputra River and shares borders with Bhutan, Bangladesh, and Myanmar. Because of its geographical location, this region encounters significant rainfall, especially during the monsoon season, with daily precipitation reaching as high as 250 to 300 mm (10 to 12 inches).A daytime temperature reaches to around 36 °C (96.8 °F), fostering greenhouse-like conditions of intense humidity and heat. The unique malty flavor for which Assam tea is renowned is shaped by the pivotal role played by its tropical climate.

Structure of a business or legal framework

1. Sole proprietorship: A private company is a form where an individual is the exclusive proprietor of the business. It constitutes a straightforward setup where the owner is one person who manages the everyday operations of the company. In this case, the business is not regarded as an independent entity. Therefore, earnings and debts are merged into their personal accounts, which are assessed and taxed at the individual level.

Advantages of the sole proprietorship structure include

• Complete Control: As a sole proprietor, you have the right to make all business decisions and do not have to negotiate with other partners, managers or shareholders as in other structures.

• Easier Startup: The creation of a private company does not necessitate completing forms or remitting a state fee. This can ease the procedure and render it more affordable.

• Simple tax reporting: Because a business is not legally a separate entity, your income tax return includes the expenses and income of the business. As an added bonus, you can use business losses to offset your earned income, which can result in a higher taxable income.

• Privacy: Sole proprietors do not have to file annual reports with state or federal governments. Therefore, the company is not disclosed in the same way as in other structures.

Modern and transgenic breeding approaches consist of modern plant breeding triangle, high-throughput phenotyping (HTP), marker-assisted selection (MAS), marker-assisted backcrossing (MABC), genomic selection (GS), genome-wide association study (GWAS), and genome editing. MAS accelerates trait identification through DNA markers, improving efficiency in selecting plants with desired traits. MABC employs molecular markers to facilitate gene introgression from donor parents (DPs) to superior recurrent parents (RPs). GS employs genome-wide markers to predict performance, revolutionizing breeding by considering both major and minor-effect quantitative trait loci (QTLs). Genome editing techniques like CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats associated protein 9) enable targeted gene modifications for desired traits. Genome editing involves selecting target genes, designing guide RNAs for Cas9, and introducing the CRISPR-Cas9 system into plants. This technology has been utilized to enhance crop productivity, sustainability, and disease resistance. Transgenic crops are designed to carry specific traits, such as pest resistance, virus resistance, abiotic stress tolerance, and nutritional enrichment. Agrobacterium tumefaciens is highlighted as a tool for transgene introduction, offering advantages like low copy number integration and stable expression. Genetic engineering remains a promising avenue for creating disease-resistant crops and ensuring global food security.

Our natural ecology comprises insects, which are known to have both advantageous and detrimental consequences. Insect pests are the main obstacles in maximizing agricultural crop output, since they directly or indirectly result in significant crop loss. The intricate relationship that phytophagous insects have with their hosts is the result of a protracted and ongoing evolutionary process. Because of this, it is impossible to completely eradicate them, but there is a hopeful fix for this issue, i.e., utilizing insect resistant crop varieties. Insect-resistant kinds are those that, with the same starting infestation level and similar environmental conditions, yield considerably higher yields of high-quality fruit than susceptible varieties. By boosting yield and reducing pesticide use, these varieties had an enormous economic benefit. There are a number of highly effective insect resistance mechanisms that the targeted insect pest cannot simply overcome. This chapter discusses various breeding strategies such as introduction, selection, and hybridization as well as some modern breeding approaches that have been used to develop many insect-resistant crop varieties that have significantly improved the world’s food supply, by reducing carbon inputs through reduced pesticide use. By expressing bacterial endotoxins and producing Bacillus thuringiensis (Bt) crops, genetic engineering also aids in the production of insect-resistant crops. Furthermore, RNA interference (RNAi) and genome editing by CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated 9) offer new avenues for the production of insect-resistant crops.

Salinity stress poses a significant threat to agricultural productivity worldwide, impeding crop growth and yield potential. Developing crop varieties with enhanced salinity tolerance is essential for ensuring food security and sustainable agriculture. Salinity stress, resulting from excessive salt accumulation in soil and irrigation water, adversely impacts crop growth, water uptake, and nutrient assimilation. Traditional breeding methods, coupled with recent advancements in molecular and genomic techniques, have enabled the development of crop varieties capable of thriving in saline environments. Genetic diversity serves as the foundation for breeding efforts, allowing the identification and selection of individuals with inherent salinity tolerance traits. Utilization of wild crop relatives and landraces provides a valuable reservoir of genetic variation for salinity stress adaptation. Incorporation of molecular markers into breeding programs expedites the selection of desirable traits. Quantitative trait loci (QTLs) associated with salinity tolerance are identified through techniques such as genome-wide association studies (GWAS) and linkage mapping, facilitating marker assisted selection. Additionally, recent strides in gene editing technologies like CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats–associated protein 9) offer precise modification of candidate genes responsible for salinity response, accelerating the breeding process. A holistic approach encompassing physiological and biochemical aspects is crucial for accurate phenotypic selection. Traits, such as ion homeostasis, osmotic adjustment, and antioxidative capacity, provide insights into a plant’s salinity tolerance potential

The sustainable management of water resources is paramount in modern aquaculture practices to mitigate environmental impacts and ensure long term viability. This chapter explores innovative methods and innovations meant to reduce water consumption in aquaculture systems. We start with a summary of the significance of water conservation in aquaculture before going into several strategies including aquaponics, biofloc technology, and recirculating aquaculture systems (RAS). Recirculating aquaculture systems (RAS) consume less water and area while producing fish in large quantities. A balance between water use, waste discharge, energy consumption, and productivity must be found to accomplish both environmentally friendly and economically viable production. The biofloc systems emphasise the significance of heterotrophic bacteria in transforming organic materials into microbial aggregates that function as a source of nutrients and a mechanism for water treatment. The creation of microbial biomass is encouraged by biofloc systems, which also maintain excellent water quality by means of carbon and nitrogen cycling. These benefits include less water exchange needs, increased resistance to disease, and increased feed conversion efficiency. In a symbiotic system, hydroponically grown plants are fertilized by fish excrement, while plants filter water for fish, resulting in aquaponics. This closed-loop method uses less water, doesn't require artificial fertilizers, and reduces environmental impact.

Introduction

The organism’s defence against infection is provided by the intricate network of organs, cells, and proteins that make up the immune system. In general, there are two types of immunological responses seen in vertebrates: the innate and adaptive immune systems. Innate immunity is the first line of defence against any foreign molecule or pathogenic invasion. Innate immunity can be classified into physical barriers, cellular and humoral factors. The physical barriers such as scales, skin, gill, gastrointestinal tract and mucus are used to prevent entry of pathogens into the body. Fish mucus contains different enzymes, complement proteins, antimicrobial peptides and IgM that are efficient in trapping and killing of pathogens (Magnadóttir B. 2006). Cellular components of an innate immune system include a variety of immune cells like macrophages, neutrophils, opsonins, lymphokines, natural cytotoxic cells, eosinophils, basophils, mast cells, eicosanoids and cytokines. They play different roles in innate immunity with their own specific pathways to prevent pathogens in the host body. Humoral factors include growth inhibitor proteins like transferrin, interferons, enzyme inhibitors, lysin, C-reactive proteins, lectins and lysozymes (Secombes and Belmonte, 2016; Roberts, 2012).

Introduction

In the past few decades, the shrimp aquaculture industry has become an increasing disease challenge, impacting huge economic losses and sustainable development. Viruses, bacteria, and microsporidian parasites are major pathogens that threaten shrimp healthcare and currently, antimicrobial resistance (AMR) pathogens, and fungi have emerging diseases in the shrimp industry worldwide.

Naturally, invertebrate crustaceans, including shrimp, depend on nonspecific immune responses mainly involved by haemolymph and hemocytic cells. A cellular immune process involving phagocytosis, encapsulation, nodulation, and prophenol oxidase leads to melanin synthesis and humoral responses such as secretion of clotting protein, enzyme inhibitors, antioxidant enzymes and antimicrobial peptides for killing or resistance against invading pathogens. Antimicrobial peptides (AMPs) are evolutionary conserved small peptide molecules ubiquitously found in all living organisms. AMPs are an integral part of the innate immune system in various organisms. These peptides have a broad spectrum of inhibitory effects against bacteria, viruses, fungi, and parasites. Shrimp AMPs are short, molecular weight of less than 13 kDa, possess diverse structures, sizes, and biochemical features, and have an amphipathic structure with cationic or anionic properties that selectively target the negatively charged membranes of microbes.

Introduction

The cultivation of flowering and attractive plants is referred to as floriculture, sometimes known as flower farming. Even though flowers have long been planted in India for a variety of purposes, including aesthetic, social, and religious purposes, the commercial floriculture sector is a relatively recent development.

The industry of floriculture has a great deal of untapped potential and has quickly become an essential part of modern life. The floriculture industry has developed into a business that is run for profit, has achieved significant market value, and is poised to realise favourable prospects. In India, the cultivation of blooming plants, including garden plants, cut flowers, foliage plants, potted flowering plants, and other types of blooming plants, is referred to as f loriculture. In today's world, flowering or ornamental plants are frequently used as part of several celebrations' decorations or as presents due to their versatility. They are also used as vital raw materials in the sectors of perfume, pharmaceuticals, and oil extraction. So, to start a floriculture firm in India, it is necessary to comprehend how agribusiness is used in its marketing and production.

Pesticide use in agriculture has evolved at several stages in the last few decades. Pesticides, as defined by the FAO, are any substance or mixture of substances intended to prevent, destroy, or control any pest, including vectors of human or animal disease, unwanted species of plants or animals causing harm during or otherwise interfering with the production, processing, storage, transport, or marketing of food, agricultural commodities, wood and wood products, or animal feedstuffs, or substances that may be administered to animals. A plant growth regulator, defoliant, desiccant, or agent used to thin or prevent premature fruit fall, as well as substances applied to crops before or after harvest to prevent them from deteriorating during storage and transportation, are included in this category.

Today’s agrochemistry has a massive challenge: ensuring the availability of high quality active ingredients to enable long-term control of pest species, weeds, and agricultural diseases. The differential uptake and transfer of the active ingredient by target and non-target organisms can contribute to diverse biological activity.

Insecticides possess toxicity towards both pest organisms and human beings. Nevertheless, the potential harm to humans and non-target animal species can be mitigated by implementing appropriate safety measures. The ingestion or prolonged dermal exposure to most insecticide s can result in deleterious effects, whether intentional or accidental. Inhalation of insecticide particles may occur during the spraying process, and there is an associated risk of contamination of drinking water, food, or soil. The issue of safety is consistently a concern when utilizing insecticide s. Exposure to insecticide concentrates or vapour drift has the potential to cause harm to applicators, bystanders, and the surrounding environment. It is imperative to exercise specific precautions when engaging in the activities of transportation, storage, and handling. Individuals who are engaged in the handling and application of insecticide s are required to possess knowledge and adhere to appropriate safety protocols in order to mitigate potential risks. The foundation of ensuring insecticide safety lies in the selection of an appropriate product. The preservation of safety is of utmost significance in the various stages of insecticide management, including storage, transportation, mixing, and loading. The safe execution of equipment cleanup and maintenance is imperative, as it necessitates the proper disposal of unwanted insecticide s and empty insecticide containers. Regular cleaning and maintenance of spray equipment is essential in order to mitigate the occurrence of leaks. Furthermore, individuals who are involved in insecticide application should undergo comprehensive training to ensure their adeptness in handling these substances safely.

Introduction

The smallest, most basic, most primitive organisms are known as prokaryotic (Greek: pro= primitive or prior, and karyon = nucleus). Likely, they originated around 3.5 billion years ago. Bacteria, such as Mycoplasma and Cyanobacteria, are known to contain these cells. A prokaryotic cell is a deeply organized single-enveloped system. Its constituent nuclear parts are encased in a plasma membrane, with the cytoplasmic ground substance encircling the center. Prokaryotic cells’ cytoplasm lacks distinct cytoplasmic organelles, the nuclear envelope, and any other cytoplasmic membrane.

Despite being incredibly thin and delicate structures, in Eukaryotes plasma membranes are essential to many of a cell’s most vital processes. Membranes are lipid-protein assemblages where non-covalent linkages hold the constituents in place in a thin sheet. Both integral protein and phospholipid lateral mobility are significantly impacted by the physical condition of the lipid bilayer. The plasma membrane is a selectively permeable barrier that permits solute passage via assisted diffusion, active transport, and simple diffusion via membrane channels or the lipid bilayer. A system of membrane organelles, such as the endoplasmic reticulum, Golgi complex, and lysosomes, is found in the cytoplasm of eukaryotic cells. These organelles are physically and functionally connected to the plasma membrane and one another. The cytoplasm is separated into a luminal region inside the ER membranes and a cytosolic space outside the membranes by the endoplasmic reticulum (ER), a network of tubules, cisternae, and vesicles. Additionally, the ER is where the majority of a cell’s membrane lipids are generated and transported to different locations. Proteins’ asparagine residues are added with sugars (a process known as glycosylation), which starts in the rough ER and continues in the Golgi complex.

Introduction

Carbohydrates are essential organic biomolecules/macromolecules composed of carbon c., hydrogen (H), and oxygen (O) atoms, typically in a 1:2:1 ratio. They are one of the four major classes of biomolecules, alongside proteins, lipids, and nucleic acids. Carbohydrates play critical roles in energy storage, structural integrity and cell signaling. Carbohydrates with an aldehyde as their functional group are called Aldoses. Those with keto as a functional group are called Ketoses.

Classification of Carbohydrates: They are broadly classified into three major groups:

1. Monosaccharides: The simplest form of carbohydrates, consisting of a single sugar unit. They cannot be hydrolyzed to yield simpler forms of sugar. They can be subdivided into trioses, tetroses, pentoses, hexoses, heptoses and octoses etc., depending upon the number of carbon atoms they possess. Important monosaccharides are:

• Glycerose / Glyceraldehyde (Aldotriose): Glyceraldehyde 6-phosphate is an intermediate in Glycolysis.

• Ribose (Aldopentose): found in Nucleic acids.

• Glucose (Aldohexose): The primary energy source for cells, known as blood sugar.

• Fructose (Ketohexose): Found in fruits (fruit sugar), a major component of sucrose.

• Galactose (Aldohexose): Known as brain sugar. It is a part of lactose sugar.

Introduction

Lipids may be regarded as organic substances relatively insoluble in water, soluble in organic solvents (chloroform, ether, benzene, ethanol and other non-polar solvents, etc.), actually or potentially related to fatty acids and utilized by living cells. Lipids are a diverse group of hydrophobic or amphipathic biomolecules that play crucial roles in energy storage, cell membrane structure, and signaling. Unlike carbohydrates and proteins, lipids are not defined by a specific polymer structure but rather by their solubility in non-polar solvents. Broadly lipids are classified based on their function as ‘Storage lipids’ and ‘Structural lipids’. Some of them are co-factors, hormones, electron carriers, light-absorbing pigments and hydrophobic anchors of proteins.

Fatty Acids: Fatty acids are straight aliphatic chains with a methyl group at one end and a carboxyl group (-COOH) at the other end. They are the simplest form of lipids.

• Saturated Fatty Acids have no double bonds between carbon atoms, leading to straight chains (e.g., palmitic acid).

• Unsaturated Fatty Acids contain one or more double bonds, causing kinks in the chain (e.g., oleic acid). Fatty acids containing more than one double bond (polyunsaturated fatty acids/PUFA) are found in relatively minor amounts.

Introduction

A pentose sugar, one or more phosphate groups, and a nitrogenous base (purine or pyrimidine) make up a nucleotide. Phosphodiester bonds, which connect the 5’-hydroxyl group of one pentose to the 3’-hydroxyl group of the next, bind nucleic acids together as polymers of nucleotides. RNA and DNA are the two different forms of nucleic acids. RNA nucleotides contain ribose, while uracil and cytosine are typical pyrimidine bases. Thymine and cytosine are common pyrimidine bases, and DNA contains 2’-deoxyribose in its nucleotides. In both RNA and DNA, adenine and guanine are the main purines. There is ample evidence to suggest that genetic information is encoded in DNA The Avery MacLeod-McCarty experiment, in particular, demonstrated how DNA obtained from one bacterial strain may infiltrate and change the cells of another strain, giving it some of the genes from the donor. The Hershey-Chase experiment demonstrated that a bacterial virus’s DNA contains the genetic instructions needed for the virus to replicate in a host cell, but not its protein coat. Watson and Crick proposed that native DNA is made up of two antiparallel chains arranged in a right-handed double helical configuration based on a compilation of numerous published data points. A-T and G-C, two complementary base pairs, are created by hydrogen bonds inside the helix. 10.5 base pairs are stacked per turn, perpendicular to the double helix’s long axis, at a distance of 3.4 Å. There are various structural configurations for DNA A- and Z-DNA are two variants of the Watson-Crick form or B-DNA The DNA molecule bends as a result of certain sequence-dependent structural differences.

Introduction

Robert Brown discovered the nucleus in some plant cells in 1831. After analyzing tissues from a range of animals and plants, T. Schwann (1839) declared, “All living organisms are composed of cells.”

The fundamental elements of Prokaryotic and Eukaryotic cells

The simplest cells, called prokaryotic cells, have a straightforward structural arrangement. There is only one membrane system in it. They contain rickettsia, spirochete, mycoplasma, virus, blue-green algae, and bacteriablue-green algae, or cyanobacteria are the largest and most intricate prokaryotes, where higher plant photosynthesis occurs and have changed throughout time.

Prokaryotic cells

Prokaryotic cells range in size from 1 to 10 µm. They take place in a range of formats. A bacterial cell is made up of three basic parts:

I. External layer: It is composed of the inner cell or plasma membrane, middle cell wall and outer slimy capsule.

a. Cell membrane: The thin, flexible membrane of the cell, composed of proteins and lipids, regulates the flow of chemicals throughout the cell. It carries respiratory enzymes for reactions that release energy. Respiratory enzymes are found in mesosomes, which are folded regions of the plasma membrane that are compared to the mitochondria in eukaryotic cells. Similarly, the pigments and enzyme molecules found in photosynthetic cells are linked to the in-folds of the plasma membrane known as photosynthetic lamella, which is responsible for absorbing light and converting it into chemical energy.

b. The cell wall: It is the non-living, semi-rigid layer that encloses the cell membrane, with a thickness that varies from 1.5 to 100 µm. It is chemically made up of peptidoglycans. Certain bacteria, such as mycoplasma, don’t have cell walls.

Introduction

Urinalysis, the examination of normal and abnormal components of urine, is one of the most important aspect of clinical pathology that aids in accurate and timely diagnosis in veterinary practice. Urine is considered as one of the most accessible bio-fluid that can be collected using non- invasive techniques in adequate quantities. It includes the evaluation of colour, odour, turbidity, volume, pH, specific gravity, protein and glucose content, ketone bodies, blood, erythrocytes, leukocytes, epithelial cells, casts, crystal, micro-organisms, etc. Urinalysis gives us valuable information regarding the several important attributes of renal function. For example, specific gravity of urine gives valuable insights over the potential of the Henle’s loop to dilute urine and the distal convoluted tubules to concentrate urine after ruling out the extra-renal factors affecting the urine specific gravity. It is highly useful to evaluate the different types of urogenital diseases and it also provides significant information regarding several systemic disorders. It is comparatively quicker to perform and is relatively a low-cost test that could be easily done in most clinical setups.

This study aimed to optimize the incorporation levels of whole egg powder (WEP) and skim milk powder (SMP) and to evaluate the storage stability of the resulting protein-rich pasta. Response Surface Methodology (RSM) with a Central Composite Design was employed for optimization; thirteen formulation combinations were tested. The best formulation was identified as 5% WEP and 10% SMP. This optimal mix resulted in a significant improvement in the proximate composition of the fortified pasta compared to the control. Both the control and fortified pasta were stored under aerobic conditions at 20-30±5°C in aluminium foil-LDPE laminate pouches for 60 days. The pasta samples were analyzed at intervals of 1, 15, 30, 45, and 60 days. Storage studies revealed an increase in TBARS, FFA, peroxide values, and microbial counts in both pasta types, but the control samples exhibited a significantly lower rate of increase (p<0.05). Sensory evaluation showed lower scores for the fortified pasta compared to the control. Despite these findings, the fortified pasta with 5% WEP and 10% SMP maintained its physicochemical, colour, textural, microbiological, and sensory qualities over the 60-day storage period. Microstructure studies revealed increased interaction between added protein (WEP & SMP) and starch molecules, indicating a potential option to produce high quality pasta with enhanced nutritional and functional properties, thereby enhancing the practical implications of the research.

Introduction

Antimicrobial resistance (AMR) represents a critical public health crisis, with far-reaching implications for human, animal, and environmental health. If unchecked, by 2050, the global toll of AMR-related deaths could exceed 10 million, with Asia and Africa alone projected to account for approximately 4 million fatalities. In a potential ‘superbug’ outbreak scenario, the economic loss could reach as high as USD 100 trillion, especially impacting low- and middle income countries (LMICs) like India (O’Neill, 2014). AMR complicates the treatment of a wide array of infections, transforming previously manageable conditions into fatal threats. In 2019 alone, AMR was directly responsible for approximately 1.27 million deaths and contributed to an additional 5 million fatalities—surpassing the mortality rates of major infectious diseases such as HIV/AIDS and malaria (Murray et al., 2022). The urgency of addressing AMR is underscored by a World Bank report, which warns that unchecked AMR could cause a global GDP decline of 1.1% to 3.8% by 2050, and averting this scenario may require an estimated annual investment of around 9 billion USD (Aslam et al., 2024).