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RNA is transcriptional product of DNA. The total RNA includes three classes, rRNA, tRNA and mRNA. The ribonucleic acids exists as ribonucleo protein particles in intact cells. The ribonucleo protein complex is dissociated by SDS in to RNA and protein deproutinised by phenol and the free RNA left in the aqueous solution is precipitated in cold by adding alcohol. 

Introduction

Rhizobia are Gram-negative soil bacteria , aerobic nitrogen fixers and adhere to the roots of leguminous plants. Fixed nitrogen is assimilated to proteins and is transported along the plants. A part of proteins is also released in rhizosphere region.

Requirements

1. Yeast Extract Mannitol Agar (YEMA)
2. Sterile saline
3. HgCl₂ solution (1%)
4. Ethanol (70%)
5. Root nodules of Mung bean
6. Water blanks
7. Pipettes
8. Petri plates
9. BOD Incubator

Under natural habitats microorganisms usually grow in mixed populations containing several species. This is really a problem for microbiologists because a single type of microorganism can not be studied adequately in a mixed culture. In laboratories these populations can be separated into pure cultures. The pure cultures contain only one type of organism and are thus suitable for studying the cultural, morphological and biochemical characters of an individual species. In the present days there are several ways of developing pure cultures in laboratories. Some of which are as follows:

Isolation of fungal pathogens

a. From leaves

•Choose leaves with young lesions.

•The lesions along with some healthy portion are cut in small pieces.

•The leaf portion is surface sterilized with 0.1% HgCl₂ for 10-15 seconds and washed with sterilized water 4-6 times.

•After washing, infected leaf pieces are placed on PDA plates with a sterilized forcep.

•After two days of incubation at optimum temperature, bits of mycelium from the margins of leaf pieces are transferred to fresh PDA slants.

•In certain cases the leaves are incubated in moist chamber and growth

•from infected portion is transferred directly to culture media.

Introduction

This method can be scaled to yield amounts of DNA ranging from less than ten to more than hundreds of micrograms of DNA. However, shearing forces are generated at every step, with the result that the DNA molecules in the final preparation rarely exceed 100-150 kb in length. DNA of this size is adequate for Southern analysis on standard agarose gels, as a template in Polymerase chain reactions (PCRs), and the construction of genomic DNA libraries in bacteriophage Lambda vectors.

Principle

The method described in protocol involves digesting eukaryotic cells or tissues with proteinase K in the presence of EDTA (to sequester divalent cations and thereby inhibit DNases) and solubilizing membranes and denaturing proteins with a detergent such as SDS. The nucleic acids are then purified by phase extractions (isopropanol). Contaminating RNA is eliminated by digestion with an RNase, and low molecular-weight substances are removed by dialysis (or centrifugation).

ABSTRACT

Amajor goal of plant biotechnology is to develop effective and lasting means to reduce crop losses due to stress caused by biological organisms. In Indian context, fungal diseases are rated as the most important factor contributing to yield losses. Although, conventional programmes towards development of disease resistant cultivars have been successful , they suf fer from limitations of human health consequences, potential degradation of environment etc. In this scenario, introduction of specific genes encoding for disease resistance through genetic engineering seems to be a viable alternative. Among the various genes that are induced in response to plant defense, chitinases are the most widely studied and used for development of transgenic crops. Keeping this in view, attempt was made to isolate chitinase genes from four fungal species viz., Trichoderma harzianum, Metarhizum anisopliae, Nomuraea rileyi and Alternaria solani by designing primers based on sequence information available in the database. RT-PCR was done using the RNA isolated from the fungal species using the designed primers. Amplified products were eluted, cloned into pTZ57R vector and sequenced. Characterization of sequenced cDNA was done using the BLAST (x) programme of NCBI site. The amplified product (261 bp) from T. harzianum showed 90 per cent homology to endochitinases from T. asperellum and T. viridae. Using this cDNA, full-length chitinase gene of 1556 bp from T. harzianum could be accomplished through RACE (Rapid amplification of cDNA Ends). In M. anisopliae the amplified product of 1500 bp matched the full length gene of 1500 bp and showed 99 per cent homology to M. anisopliae and 96 per cent homology to M. flavoviride. In other two fungal species, viz., Nomuraea rileyi and Alternaria solani, the amplified products did not show any homology to chitinase gene

Mesenchymal stem cells are multipotential cells capable of proliferation and differentiation into osteogenic, chondrogenic and adipogenic lineages both in vitro and in vivo. Minimal criteria for defining MSC according to International Society of Cell Therapy (ISCT) are (a) they must be plastic-adherent when maintained in a standard culture conditions, (b) MSC must express some surface antigen such as CD105, CD73, CD90, CD44 and must not express CD34, CD45, CD 14, CD19, and HLA-DR, and (c) MSC must differentiate in vitro into osteoblast, adipocytes and chondrocytes under specific differentiating condition.

Although traditionally MSC isolated from bone marrow, more recent reports have detailed the isolation of cells with MSC characteristics from a variety of tissues including umbilical cord blood, chorionic villi of the placenta, Wharton’s jelly, peripheral blood, fetal liver and lung, adipose tissue, skeletal muscle, periosteum, deciduous teeth, amniotic fluid, dental pulp, testis, synovium and the circulatory system. Bone marrow and periosteum sources are richest in young animals with their numbers diminishing, but still present in old age. It is estimated that MSCs represent approximately only 0.01 to 0.001% of the total nucleated cells within isolated bone marrow aspirate. The yield of MSCs from bone marrow varies from species to species. e.g., Canine =1 in 2.5 × 10⁴; Feline =1 in 3.8 × 10⁵; Murine =1 in 10.8 ×10³ to 1 in 3.45×10⁴ and Horses =1 in 4.2 × 10³. Despite this low number, they are remains a great interest, as they can be easily isolated from a small aspirate and culture-expanded through as many as 40 population doublings to significant numbers in about 8 to 10 weeks.

Mesenchymal stem cells are multipotential cells capable of proliferation and differentiation into chondrogenic, osteogenic and adipogenic lineages. Minimal criteria for defining MSC according to International Society of Cell Therapy (ISCT) are (a) they must be plastic-adherent when maintained in standard culture conditions, (b) MSC must express some surface antigens such asCD105, CD73, CD90, CD44 and must not express CD34, CD45, CD 14, CD19, and HLA-DR, and (c) MSC must differentiate in vitro into osteoblast, adipocytes and chondrocytes under specific differentiating condition. 
Although MSC is traditionally isolated from bone marrow, more recent reports have detailed the isolation of cells with MSC characteristics from a variety of tissues including umbilical cord blood, chorionic villi of the placenta, Wharton’s jelly, peripheral blood, fetal liver and lung, adipose tissue, skeletal muscle, periosteum, deciduous teeth, amniotic fluid and synovium. Bone marrow and periosteum sources are richest in young animals with their numbers diminishing, but still present in old age. One of the attractive advantage of bone marrow derived mesenchymal stem cells (BM-MSCs) as a source of cell transplantation is their low immunogenecity. Recently, several studies have reported that BM-MSCs may be immune-privileged cells that do not elicit immune response due to an absence of their immunologically relevant cell surface markers. BM-MSCs also are known to inhibit proliferation of T lymphocytes, B lymphocytes, dendritic cells and natural killer cells.

What is DNA

DNA is a simple molecule

DNA is negatively charged

DNA has lots of ring structures; ring structures are generally quite hydrophobic.

DNA is a very robust molecule.

DNA work has lots of toys.

Although it can be argued that due to its overall trendiness, they have been a lot of recent developments in making it more fun to work with. As a molecule, it is very similar to DNA (the most notable chemical difference is its flexibility), but suffers because the nucleases (RNases) that can destroy it are notoriously very stable and very difficult to get rid of.

Plasmid is a double stranded, self replicating, circular extra chromosomal DNA of bacterium. It is used in recombinant DNA experiments to clone genes from other organisms and make large quantities of their DNA. Plasmid can be transferred between same species or between different species. The sizes of plasmids range from 1-1000 kbps. Plasmids are part of mobilomes (total of all mobile genetic elements in a genome) like transposons or prophages and are associated with conjugation.

There are various methods available for isolation of high molecular weight genomic DNA from plants. The basic steps involved in all these methods are removal of cell wall, nuclear membrane, proteins, lipids, carbohydrates, cell debris, RNA without affecting the integrity of DNA. The two major factors that affect DNA integrity are action of endogenous nucleases and impact of mechanical shearing.

After precipitation, run-off and surface water storage in lakes and man-made reservoirs, the next steps in the hydrological cycle are infiltration through the unsaturated zone and groundwater dynamics in the saturated zone. Well-established applications of isotopes in groundwater hydrology are

The range of isotope applications in surface water hydrology is not as wide as in groundwater hydrology as the surface waters are more easily amenable to conventional hydrological methods. Following are the areas in which isotopes have a significant role in either improving the accuracy of measurements or in providing additional information, which is otherwise not easily available.

For the first time, isotope dilution analysis was introduced by Hevesy and Hafer in 1934, but it was not until 1940, the usefulness of isotope dilution technique was revived by Rittenberg and Foster (1940). There are three general types of isotope dilution methods. These are (a) direct (b) inverse and (c) double isotope dilution. In general isotope dilution methods may be used for the following type of studies.

    (a)    In isolation purification and identification of unknown intermediate in a chain of metabolic reactions.

    (b)     To obtain evidence of synthesis (incorporation), and precursor-product relationships between known compounds.

    (c)    As an analytical tool in the assay of known compounds. In isotope dilution techniques, the labelled intermediate, which is being diluted by unlabelled carrier, is a known compound.

2.1 Isotopes

In 1913, J.J. Thomson and Aston introduced atmospheric neon into the positive ray apparatus and got two parabolas corresponding to masses 20 and 22, with the intensities of the tracks corresponding to the ratio of 9:1, irrespective of the condition of discharge and pressure. This indicated that neon existed in two forms having atomic weights 20 and 22, and at the same time it could not be distinguished chemically. These two types are known as isotopes of neon (iso = same, topas = place in the periodic table). When the average atomic weight of the ordinary neon was calculated based on the abundance of these two forms in the ordinary neon, it was found to be 20.2 which agreed the chemical methods. Thus the existence of isotopes of a given element was discovered.

Formerly isotopes were defined chemical properties but with different atomic weights, the same atomic number and occupying the same position in the periodic classification. Later this definition was slightly modified and now isotopes are defined as nuclides with the same number of protons but differing in the number of neutrons, for example, ordinary hydrogen (also called protium ; proton =1 and neutron =0), deuterium (heavy hydrogen ; proton =1 and neutron = 1), and tritum super heavy hydrogen (proton =1 and neutron =2). Investigations using mass spectrographs have revealed more than 280 isotopes which are stable artificially radioactive isotopes.

4.1 Selection of Isotopes for Tracer Studies

In radioisotopes we have all the essentials of a powerful research tool, a non-destructive means by which atoms could be followed and detected in the organism. Application mainly depends on the fact that chemical properties of the isotope of an element are essentially identical.

The basic requirement of a tracer is that it be

1.Chemically and physically, virtually equivalent to the substance it represents or displaces and that it, in no appreciable way, affect the system differing from its normal counterpart.

2.Radioactivity could be detected directly and

3.Unaffected by the physical or chemical treatments that are given. Hence, this has been used widely in the investigations of the various problems in biology, chemistry, physics and other fields like agriculture.

1. INTRODUCTION

The contribution of Extension and research to Israel’s agricultural productivity and profitability is a collaborative effort with research hailing back to the late 19^th century. It facilitated technological innovation in agriculture, transfer of information, training of farmers and connectivity. These were in effect a dictate of continuously deteriorating agricultural terms of trade, crippling water shortages and severe climate and soil constraints.

The Israeli Extension Service’s (IES) adoption of Information and Communication Technologies (ICTs) significantly enhanced extension’s capabilities and scope of activities. ICT empowered extension’s ability to develop, introduce and adopt innovative agricultural technologies and collaborate with research and other partners. Adoption of ICT as one of the tools of technological innovation dramatically improved the transfer and management of information, production chain efficiencies and integration within and with the agricultural sector. These were and are critical success factors contributing to Israeli Extension, Research, Services and Farmers ability to sustain a profitable and thriving agriculture sector. As such the overall results are a major contribution to rural viability and an illuminating case study of technological innovation adoption.

Introduction

The concept of food security having relevance at global, national, regional, state, household and individual level is well understood as a core issue over the last three decades in the world. The World Food Conference of 1974, organized in the wake of the world food crisis of 1972-74, was largely concerned with global food security. It was felt as the common responsibility of all nations that international approaches were needed to achieve the goal of improved world food security. The food security at national level means arrangements for providing physical supply of a minimum level of food during all periods including those having harvest failures. FAO (1983) had formulated that the basic concept of food security implied that all people at all times have physical and economic access to the basic food they need. The World Bank (1986) has modified this formulation to indicate that food security is access by all people at all times to enough food for an active healthy life.

In this direction, the conference on Environment and Development held in Rio (1992), the Joint FAO/WHO Conference on Nutrition held in Rome (1992), the World Conference on Human Rights held in Vienna (1993), the International Conference on Population and Development held in Cairo (1994), the World Summit on Social Development held in Copenhagen (1995), the Fourth World Conference on Women held in Beijing (1995), the Habitat II Conference held at Istanbul (1996), the World Food Summit held in Rome (1996), etc. deliberated on various facets of food security like food security and sustainable development, right for nutritionally fit and safe food, population growth and food production, global measures to satisfy the food needs, campaign against hunger, women in food production, rural-urban linkages and so on.

9.1. Challenges for Nigeria

Nigeria, at independence in 1960, was largely a producer and net exporter of primary products. The six major agricultural products then were cocoa, rubber, palm oil, groundnut, cotton and palm kernel. Although there existed mining and quarrying activities, these were of negligible percentage and never counted for the economy as a whole. In other words, agricultural produce and raw materials constituted the sole foreign exchange earner for the country. Specifically, the Nigerian state as an exporter of agricultural goods had 69.4% of its total GDP for the year 1963/64 comprising the six aforementioned agricultural commodities (Olaloku, 1979:8).

The trend of having agriculture as the main foreign exchange earner for Nigeria stopped in the early 1970s when the country was suddenly awash with petrol dollars arising from the quadruple increase in the price of oil in the world market. From 1972 onwards, oil gained ascendancy over all other commodities as the largest contributor to the GDP, and also as a major foreign exchange earner. There was substantial increase in oil production accompanied by a sharp increase in the global market price of high-grade crude oil from low price of $3.8 per barrel in October 1973 to a skyrocketing price of $14.7 per barrel in January 1974. This trend continued till 1981 when the price of crude oil attained a high level of $38.77 per barrel. Within the same period, total revenue from oil rose correspondingly from ? 1 billion to ? 4 billion while external reserves increased from ? 180 million to ? 3.7 billion in 1975 (Osaghae, 1998:96).

19.1. Remedies to Combat Unemployment in Nigeria

(a) Rural development

One way of stemming the rural-urban drift, and thus control the resulting unemployment in our urban centers, it for the government to develop the rural areas. Not only should amenities be provided, but jobs should be created in the rural areas to stem the exodus. Fortunately, the Babangida administration seemed to have realized this with the huge amount allocation for the development of rural areas in the 1986 and 1987 budgets.

(b) Population control

Government must intensity the effort to control population growth Nigeria already has a huge population and with an estimated growth rate of about 3 percent annually, this can only compound the unemployment in the face of inadequate resources. It is therefore advocated that the government should renew the family planning campaign to check the population growth.

ABSTRACT

This paper discusses IPR concepts in the light of Indian culture and issues emanating from enforcement of those concepts in developing countries such as India.

INTRODUCTION

The adage “Knowledge is wealth” suits the present era well than any other period. India is known for its innovative thoughts since ages. However, it never monopolized the knowledge it possessed. In India, the knowledge of a person is thought to be utilized for the benefit of the society as an obligation of the knowledge holder. Does this mindset of Universal humanity (Vasudevo Kudumbagam) and common good (Sarva Sugino Bavandu) would benefit us in the materialistic society? This paper is an attempt to answer this query.

Yak is among the most remarkable domestic animals. It is adapted to thrive in the harsh environment of high altitude and sustains livelihood of highlanders. Yak is handicapped because of lack of veterinary assistance and declining pastoral biomass. Marketing facilities for yak-based products are limited and the production potential of yak husbandry is on decline. Dearth of locally available trained manpower and prevailing illiteracy are other hindering factors for development of yak husbandry. Research and development with focus on upliftment of yak husbandry and yak products are therefore important.

Pulse production in NE states in still too low as compared to whole India. However, there is very good potentialfor enhancing pulse producity in the region and contribute to food grain production of the country. In view of these constraints, prospects for increasing pulse production from this region have been visualized.

Pulse production scenario

Despite immense importance of pulses in humanand animal nutrition, the share of north-easternregion inthe total pulse areofIndiaistoolow (0.76%)andthisregion contributesonly176.44 thousandtons(0.99%) in the all Indiapulse production (Table 1). WithinNE Region, mostof the pulsearea isoccupied by Assam and 47% of total N.E. Region’s pulse production is contributed by this state alone. After Assam, Nagaland and Tripura are major pulse growing states with 33.5 and 14.5 thousand hectares of area and 36.46and 24.20thousandtons ofproduction, respectively. In Meghalaya, pulseswere grownon4 thousandhaarea and4.2 thousand tonnesof pulses wereharvested in 2010-11. Thefactthat theaverage pulse productivity of NE region (883.0kg/ha)is much higher thanthatof whole India (689kg/ha) offers opportunityfor increasingarea and production of pulses in the region.

Introduction

Definition of ‘urban’ areas varies from country to country. Consequently, it is difficult to provide a universally acceptable meaning of the term. The difficulty has been summarised by UN (1998) as follows: “because of national differences in the characteristics that distinguish urban from rural areas, the distinction between urban and rural population is not amenable to a single definition that would be applicable to all countries” (UN, 1998). In many cases the divide between a rural and an urban area is based on the minimum population, but the number varies widely between the countries, e.g., 200 in Iceland, 2000 in some countries of West Asia, 5000 in India, and 10000 in Italy! Despite this challenge, most towns and cities are historical phenomena and have their defined administrative boundaries for services and other purposes, including municipalities. These places straight away qualify as the urban centres.

In India, the word has been defined by the Census of India (2001) as: “(a) All statutory places with a municipality, corporation, cantonment board or notified town area committee, etc.; (b) A place satisfying the following three criteria simultaneously: (i) a minimum population of 5,000; (ii) at least 75 per cent of male working population engaged in non-agricultural pursuits; and (iii) a density of population of at least 400 per sq. km (1,000 per sq. mile).” This definition was adopted for the 2011 Census.

4.1. Issues in Employment
4.1.1. What is employment?

Employment is a relationship between two parties, usually based on a contract, one being the employer and the other being the employee.

(a) Employee

An employee contributes labor and expertise to an endeavor of an employer and is usually hired to perform specific duties which are packaged into a job. In most modern economies, the term “employee” refers to a specific defined relationship between an individual and a corporation, which differs from those of customer or client. Other types of employment are arrangements such as indenturing which is now highly unusual in developed nations but still happens elsewhere.

Nigeria: Economic and Non-Economic

6.1. Issues in Growth and Development in Nigeria

Nigeria has attempted through various reform programmes to better the lots of her citizens by providing dividend of development, such as education, health, social infrastructure. Broadly speaking, the features of an underdeveloped economy create obstacles in the way of economic development, and hamper economic progress. These features emerge out of economic, social, political, religious and institutional factors. It would be wrong however to conclude that only economic factors are responsible for poverty or economic backwardness of a country. Non-economic factors are equally responsible for the under development of an economy. The factors discouraging economic development may be classified into economic and non-economic factors which are:

As stated before, a careful assessment of the existing curriculum is essential before any modification is undertaken and must take into account the country’s education policy, political, economic, demographic, cultural, social and technological environment. Problems of farmers to be solved, needs to be satisfied, and priorities to be identified and addressed and then only the recommendations made for action.

Pesticides are any chemical or mixture intended for use as a plant regulator, defoliant, or desiccant as well as any substance or mixture meant for preventing, getting rid of, repelling, or controlling pests. Agricultural pests include insects, weeds, fungi, bacteria, viruses, nematodes, rodents, and mollusks. The term pesticide, which refers to these pests and cide, which meaning “to kill.” Insecticides, fungicides, bactericides, weed killers, molluscicides, rodenticides, nematicides, and other types of pesticides can be classed as pesticides. Every manufacturer of pesticides as well as companies with CIB & RC registration must provide all scientific data on bio-ecacy, toxicity, residues, and safe waiting periods that has been obtained via laboratory research.

Genetically engineered crops refer to alterations in the genetic makeup of the crop by introgression new traits such as herbicide tolerance, virus resistance, drought, flood and frost resistance, delay in maturation time of the crop and increased crop yield. They can be made resistant to pests and diseases which can significantly reduce the consumption of insecticide. Biodiversity is the feedstock for biotechnology industries. The issues relating to the Genetically Modified (GM) Foods have generated intense public debate in many parts of the world. Even though the issues under debate include the costs and benefits of the GM crops and the inherent safety concerns, the outcome of the debate differs from country to country, depending on its geographical location, strength and resilience of the farm sector, attitudes of people towards food, and so on (Anonymous, 2009). In India also, this debate has engaged the attention not only of the Government but also of the farming community and the civil society.

INTRODUCTION

Potato plays a vital rolein foodsecurityworldwide. It is the third most important food crop, afterrice and wheat. It is a nutrient-rich crop which provides more calories,vitamins and nutrients per unit area than any otherstaple crops. FAO declared potato as the crop to address future global food security and poverty alleviation during2008. In Indian agriculture, potatoplays a very important role as it alone contributes about 21 percent of the total vegetable area and 26 percent of total vegetable production of India (DAC&FW, 2017a). As per FAOSTAT data for the year 2017,India with48.6 million t is ranked second in potato productionin the world, onlybehind Chinawith 99.2 million t (Figure 1).

1. INTRODUCTION

Organic farming systems can deliver agronomic and environmental benefits both through the structural changes and the tactical daily management of the farming system. The benefits of organic farming systems are relevant both to developing and developed nations which helps for environmental protection, biodiversity enhancement, reduced energy use and CO₂ emissions (Thiyagarajan, et al., 2003). The post Green Revolution era has led to environmental pollution due to excessive use of agrochemicals and fertilizers and thus threatened the fragile ecosystem (Selvaraj et al., 2001). Organic farming conserves soil fertility and curtails soil erosion through implementation of appropriate conservation principles (Thampan, 1995). Organic farming leads to live in harmony with nature.

United Nation experts indicate that organic foods have the good opportunities for developing countries to open a space in the global market. This entails the creation of greater awareness of trade opportunities for organic products as well as of the environmental, economic and social benefits to the public.

Technical Knowledge (ITK) based special inputs / Techniques for plant growth

Growth of the plant has for long been believed to be due to the minerals absorbed from the soil and the food materials synthesized by the palnt. It is recognized that growth of the plant is very much regulated by certain chemical substances known as growth regulators. These substances are formed in one tissue or organ of the plant and are then transported to other sites where they produce specific effects on growth and development. These materials can be produced organically for improving plant growth.

3.1. Growth Promoting Substances

Are those organic solutions (like Panchagavya, Buttermilk & Coconut milk slurry, Amudhakaraisal) prepared using the plant and animal products, decayed fruit and other agricultural wastes. For example

From very early times, humans were astonished, fascinated and frightened by the weather events and the dynamism of changes that weather brought around them. Human beings have attempted to predict the weather informally for millennia, and formally since the nineteenth century. He has tried to predict or forecast the weather mainly based on many indicators around. In the earliest record of weather forecast, the Babylonians have predicted the weather from cloud patterns as well as astrology in 650 BC. In 904 AD, Ibn Wahshiyya, an Iraqi alchemist and agriculturist in his work Nabatean Agriculture have discussed the behavior of plants and animals also along with atmospheric, planetary, solar and lunar signs in weather forecasting. This is perhaps the first work that have discussed about the animal and bird indicators for weather forecast. Though there are a variety of end uses to modern weather forecasts, indigenous knowledge and application of weather forecasts were mainly for agricultural operations. Farmers have to rely on weather forecasts to decide on what work has to be done on any particular day. They are astute weather watchers and are quick to recognize weather that is either favourable or unfavourable to their production systems. In traditional weather forecasting, the farmers has identified a set of indicators and developed a reliability factor for each of them. Predicting weather is also an important cultural component for farmers, as it is common for farmers to discuss weather indicators on the street, markets and with family members.

Farmers have used traditional knowledge to understand weather and climate patterns in order to make decisions about crop and irrigation cycles. This knowledge has been gained through many decades of experience, and has been passed on from previous generations, and the knowledge is also adapted to local conditions and needs. But the scientific forecasts are formulated at a much larger scale and so access to get location specific rainfall forecast to take proper decision at the farm level is very limited. Besides forecasting weather, the farmers has also evolved several coping strategies in rain-fed systems across the world and so today many international development agencies, universities and research institutions have started focusing on indigenous knowledge and have incorporated it in development perspectives.

Agricultural scientists and policy makers have understood that continuation of modern agriculture might lead to severe ecological and economic problems. We are also convinced that modern agriculture may not be able to meet the requirements of the ever increasing population in the future. So, we are searching for alternative technologies. Several alternatives have been proposed such as low external input agriculture, sustainable agriculture, organic farming, biodynamic farming etc. However, they require, some times little or considerable external inputs whose availability may be uncertain in future.

Hence for the developing countries, the other alternative viz., traditional methods have special advantages over modern agricultural techniques. Also the capital and technological skill requirements in the use of traditional technologies are generally low and their adoption often requires little restructure of the traditional societies. The traditional technologies are nothing but indigenous technical knowledge. By adopting such indigenous knowledge, our ancestors did not face any problem of large-scale pest out break or economic crisis unlike the today’s farmers.

Key Points

• Animal euthanasia is the act of humanely killing animals, most commonly with injectable drugs.

• Euthanasia techniques vary by species, age, and the health status of the individual animal.

• Death must be confirmed before disposal of any animal remains.

• Animal remains must be disposed of in a legal manner that does not contaminate food sources or the environment.

Introduction

Euthanasia is the act of inducing humane death in an animal by a method that induces rapid loss of consciousness and death with a minimum of pain, discomfort, or distress. Euthanizing agents cause death by three basic mechanisms: (1) direct depression of neurons necessary for life function, (2) hypoxia, and (3) physical disruption of brain activity. Because loss of consciousness resulting from these mechanisms can occur at different rates, the suitability of a particular agent or method will depend on whether an animal experiences distress before loss of consciousness. Unconsciousness, defined as a loss of individual awareness, occurs when the brain’s ability to integrate information is blocked or disrupted. Ideally, euthanasia techniques cause rapid loss of consciousness, followed by cardiac or respiratory arrest and death.

Syn.cordifolia Cogn.,C.indica Wight and Arn,Bryonia grandisL. Family.Cucurbitaceae) is an aggressively climbing vine that  spreads quickly over trees, shrubs, fences or other supports. It is also called  scarlet fruited gourd or little gourd. The name is derived from the Latin coccineus, meaning scarlet,in reference to fruit colour (Wagner et al.,1999). Vernacular names are kundru (hindi), kovakka, kova (malayalam), kovakkai, kovai(tamil), thondakayi (kannada), dondakaya (telugu). Coccinia shoot is a good source of calcium, iron, and fiber and has good texture (Anon., 2002). It is grown mostly in homestead farms for tender fruits in almost all regions of the country. It bears fruits almost through out the year where mild winter prevails. In regions where severe winter occurs, it bears fruit for 8-9 months. Although this is considered as a an under utilized vegetable, it is grown extensively in Chattisgarh, West Bengal, Bihar and Karnataka on a commercial scale and giving remunerative returns to  farmers. Availability of better cultivars and knowledge of  many other preparations and uses of this crop can propel its evolution from an under utilized species to an important horticultural crop in Indian Agriculture.

Ivy gourd (Coccinia grandis (L.) Voigt), a climbing, herbaceous perennial plant in family Cucurbitaceae, grows well under hot-humid conditions. The genus name is derived from the Latin coccineus, which means scarlet in reference to the fruit color. Thus, it is also called scarlet-fruited gourd. Other vernacular names are: little gourd in English, courge écarlate in French, pepino cimarrón in Spanish, tam leung or phak tam leung in Thai, kundree or kundru in Hindi, pepasan in Malay, hong-gua in Chinese, and tindori in East Africa (Schippers, 2002; MMPND, 2007; USDA, 2007). The plant is often collected wild or cultivated in home gardens in tropical Asia, and its tender shoots are consumed as potherb (Wasantwisut and Viriyapanich, 2003). Bundled tender shoots of ivy gourd in the village market setting are common scenes in Southeast Asia (Boonkerd et al., 1993; Engle et al., 1998). The fruit of its sweet type cultivar is also used widely as a vegetable in India (Ramachandran and Subramaniam, 1983; Sarnaik et al., 2001; Bharathi, 2007). Ivy gourd has a potential to be a valuable vegetable in the tropics to diversify cropping system in peri-urban and urban agriculture, and to improve diet and health, especially where vitamin A deficiency is of developing concern.

Ixora is an ornamental plant which is a medium-sized, tropical flowering, evergreen shrub that is used in landscapes. The genus Ixora belongs to the family Rubiaceae. The plants have glossy green leaves which are arranged oppositely along each stem. All Ixoras produce clusters of star-shaped flowers on stem tips or terminal growth. They are grown for their beautiful flowers which bloom in summer in clusters of different shades of red, pink or yellow (De Block, 1998 and Husain and Paul, 1988). The colour of the wild flowers is red or reddish-orange but ornamental varieties may have white, yellow, salmon or pink flowers. It is commonly known as West Indian Jasmine. The other common names of this plant are Jungle Geranium, Flame of the Woods, Jungle Flame, Burning Love, Red Ixora, Ixora, Amor Ardiente, Cruz de Malta, Dola de Coral, Equisosea and Rajana (Griffiths,1994 and Liogier,1997), Rangan, Kheme, Ponna, Chann Tanea, Techi, Pan, Santan, Jarum-Jarum and many more. Members of Ixora prefer acidic soil and are suitable for bonsai (Frommer, 2001). I. coccinea is used extensively for landscaping and is also grown as a potted ornamental (Lakshmanan et al., 1997). Ixora coccinea and Ixora parviflora are generally used as the ornamental plants in gardens and parks.

Ixora is an ornamental plant which is a medium-sized, tropical flowering, evergreen shrub that is used in landscapes. The genus Ixora belongs to the family Rubiaceae. The plants have glossy green leaves which are arranged oppositely along each stem. All Ixoras produce clusters of star-shaped flowers on stem tips or terminal growth. They are grown for their beautiful flowers which bloom in summer in clusters of different shades of red, pink or yellow (De Block, 1998 and Husain and Paul, 1988). The colour of the wild flowers is red or reddish-orange but ornamental varieties may have white, yellow, salmon or pink flowers. It is commonly known as West Indian Jasmine. The other common names of this plant are Jungle Geranium, Flame of the Woods, Jungle Flame, Burning Love, Red Ixora, Ixora, Amor Ardiente, Cruz de Malta, Dola de Coral, Equisosea and Rajana (Griffiths,1994 and Liogier,1997), Rangan, Kheme, Ponna, Chann Tanea, Techi, Pan, Santan, Jarum-Jarum and many more. Members of Ixora prefer acidic soil and are suitable for bonsai (Frommer, 2001). I. coccinea is used extensively for landscaping and is also grown as a potted ornamental (Lakshmanan et al., 1997). Ixora coccinea and Ixora parviflora are generally used as the ornamental plants in gardens and parks.

It is also a popular choice for hedges in parts of South East Asia like Thailand. Red Ixora flowers are commonly used in Hindu worship as well as in Indian folk medicine. The flowers, leaves, roots and the stems are used to treat various ailments in the Indian traditional system of medicine, the Ayurveda. The fruits are used as a dietary source. The plant possesses antioxidative, antibacterial, gastroprotective, hepatoprotective, antidiarrhoeal, antinociceptive, antimutagenic, antineoplastic and chemopreventive effects. The oil extracted from the flowers is helpful in conditions like scabies, eczema, pruritis and other skin infections. It is also used for hedges and borders and planted as an indoor potted plant. In laboratory tests, extracts of Jungle Geranium have shown anti-bacterial (Kumer et al., 1997) and anti-tumour (Serrame and Lim-Sylianco, 1995 and Latha and Panikkar, 2000) activity. From a methanol extract of the flowers, 13 chemicals were identified including ursolic acid which has known antitumor and antiviral activity (Monteath et al., 2001). In the traditional medicine of India, infusions of the leaves and juice from the roots are used to treat a wide variety of ailments including dysentery, ulcers, and gonorrhea (Parrotta, 2001).

Jasmonic acid: Jasmonic acid is derived from the fatty acid, linolenic acid. It is a member of the jasmonate class of plant hormones. It is biosynthesized from linolenic acid by the octadecanoid pathway. The major function of JA and its various metabolites is regulating plant responses to abiotic and biotic stresses as a well as plant growth and development processes.

Jaeschkea oligosperma Knobl. (Gentianaceae): Ladakhi- Tikta [W]. Annual herb, along scrubby alpine slopes of Ladakh. Leaves lanceolate, blunt. Flowers bluish to reddish purple. The plant is consumed raw as blood purifier.

Judgment sampling: Judgment sampling is utilized frequently during qualitative research to develop hypotheses rather than to generalize to larger populations. The research worker’s decision is used for selecting items which are considered as representative of the population in judgment sampling.

Jaggery: Raw brown / unrefined brown sugar in lump made from palm sap (Plate 365).

Jam: A preserved product prepared on boiling pectin contained in fruit pulp with required amount of sugar, water and acid to a reasonably thick consistency, firm enough to hold the fruit tissues in position (Plate 366).

Jam pack: The packing of the commodity tightly or in excess is called Jam pack.

Jamaica rum: Heavy bodied rum made by slow fermentation and marked by a pungent bouquet.

Jamboline: A type of glucose found in the jamun fruit help in cure of the diabetes in the patients.

Jelly: A product prepared by boiling clear extract of fruits with sugar. It should be transparent, well set, and tender enough to quiver but not flower and when cut; it should retain its shape and show a clean cut surface (Plate 367).

Canavalia ensiformis is a vine that originated in South and Central America and has since spread throughout the tropics and subtropics of the planet. It’s used as a food source, fodder, and a cover crop or green manure. As a minor vegetable crop, jack bean (Canavalia ensiformis), commonly known as horse bean, is extensively dispersed in the tropics and subtropics. Jack bean is a semierect annual herb with a bushy appearance. It is only grown on a small scale in India’s eastern and north-eastern regions. Immature pods and seeds are cooked and eaten like a vegetable. The pods should be cooked in water for 15 minutes before cooking, and then the boiling water should be drained.

Genus : Artocarpus 
Species : heterophyllus L. (Lamk.)
Family : Moraceae
Chromosome No. : 2n = 56

Afamous traveller John de Marignolli has described jackfruit as ‘there is again another wonderful tree, which produced fruit on the trunk, and was very pleasing to see and the fruit was as big as a lamb‘. The rind is hard and has to be cut open by a sharp knife, the pulp inside is of pleasant flavour sweetness of which matches the honey and usually contains 500 or more chestnut like seeds which are eaten after roasting. In its place of origin (India) where it has been grown since ancient times it is held in great esteem. Theopharatus and Pliny who wrote about jackfruit also expressed similar opinion about the fruit. In India several vernacular names are given like Kanthal, Kathal, Panasa and Kantaka.

The jackfruit, Artocarpus heterophyllus Lam. of the family Moraceae, is also called jak-fruit, jak, jaca and, in Malaysia and the Philippines, nangka; in Thailand, khanun; in Cambodia, khnor; in Laos, mak mi or may mi; in Vietnam, mit. It produces the largest known edible fruit. In South-India, it is a common shade tree for many plantation crops. A jackfruit contains 28.7% pulp, 11.5% seed and 59.8% rind, sheath and core.

The place of origin of Jackfruit is not knows exactly, but it is believed to be indigenous to the rainforests of the Western Ghats of India. It is found at low elevations throughout India, Burma, Ceylon, southern China, Malaysia, and the East Indies. It is common in the Philippines, both cultivated and naturalized. It is grown to a limited extent in Queensland and Mauritius. In India it grows in a wild and semi-wild state in Assam, West Bengal, Tripura, Bihar, Uttar Pradesh and states along western Ghats range, particularly Kerala, Karnataka and Maharastra.

Cultivars: Rasdar, Khajwa, Rose-scented or Gulabi, Champa, Hazari, Rudrakshi.

Uses

Both mature and immature fruits as well as seeds are used for various purposes. Ripe fruit is used for dessert purpose and processing into various products, while unripe bulbs are used for making chips. The immature fruit and seeds are used as vegetable. The bark is used for tanning purpose. Heart-wood yield a yellow dye. A yellow dye can also be extracted from the wood particles and used to dye cotton. The latex which flows from all parts of the plant when injured is also used as adhesive. The resins within the latex may also have some value in varnishes. The timber is a medium hardwood with desirable characteristics for making furniture, oars, implements and musical instruments and the wood is also used in construction. It is termite proof and fairly resistant to fungal and bacterial decay. The roots of older trees are good materials for carving and picture framing Leaves are utilized as a green fodder. Several processed products, such as squash, nectar, fruit bar, toffies, jams, canned and dehydrated pulp, beverages, etc. are prepared from the pulp. The seeds can be eaten boiled, roasted or dried and salted as table nuts, or they can be ground to make flour and blended with wheat flour for baking (International Centre for Underutilized crops, 2003).

The jackfruit tree or jack or simply known as jak (Artocarpus heterophyllus) belongs to the mulberry family (Moraceae) and its fruit, jackfruit, is known as the “poor-man's food” (Bose, 1985; Haq, 2006; Ramanani and Rao, 2001; Rowe-Dutton, 1976; Sonwalkar, 1951; Verheji and Coronel, 1992). It is believed to be native to Southwestern India, Bangladesh, Nepal and Sri Lanka (Answers.com, 2009). It is possibly also native to the Philippines and also East to the Malay Peninsula. The fruit is large, oval and covered with a thick rind that has hexagonal protrusions. Inside the fruit, we find the yellow, fleshy and banana-flavoured carpel or large "bulbs" (which are botanically the perianths) massed among narrow ribbons of thin, tough undeveloped perianths (or perigones), around a central, pithy core. Each bulb encloses a smooth, oval, light-brown coloured "seed" (endocarp) covered by a thin white membrane (exocarp).

Jackfruit (Artocarpus heterophyllus Lam) is a multi-purpose tree species that provides food, timber, fuel, fodder and medicinal and industrial products. It is one of the species that has been brought into wider cultivation through a farmer-driven domestication process because its products meet the household needs of families with limited land, which require wood and animal feed to ensure family food security. Despite such vast potential and usefulness, jackfruit remains an underutilized fruit species and deserves to be given the necessary thrust for research and development. There is little progress on the genetic improvement of the species, including study on its reproductive biology, even though it demonstrated substantial use and importance. Therefore, a brief background on the reproductive biology of jackfruit is covered here before dealing with its genetic improvement.

Jackfruit is believed to be indigenous to the rain forests of the Western Ghats of India and the name “Jack fruit” is derived from the Portuguese word Jaca, which in turn, is derived from the Malayalam language term, Chakka. The jackfruit has played a significant role in Indian agriculture for centuries. Archeological findings in India have revealed that jackfruit was cultivated in India 3000 to 6000 years ago. During the season, each tree bears as many as 250 large fruits, supposed to be the largest tree-borne fruit in the world. The fruit varies widely in size, weigh from 3 to 30 kg and has oblong or round shape measuring 10 cm to 60 cm in length and above 25 to 75 cm in diameter.