eBook Chapters

Introduction

North East India, a mega-biodiversity centre and a hotspot (Myer et al., 2000) occupy 7.7% of India’s total geographical area supporting 50% of the flora (ca. 8000 species) (Rao, 1994), of which 31.58% (ca. 2526 species) are endemic (Nayar, 1996). It is a transitional zone between the Indo-China and Indo-Burma–Malaysian regions. This region is also a part of the vavilovian centre of biodiversity and origin of many important cultivated plant species and some domesticated animals (Agarwal, 1996). North Eastern Hilly (NEH) region of India supports a rich biodiversity spanning from tropical rainforests to alpine scrubs.  Takhtajan (1969) described the region as the “Cradle of Flowering Plants” because of its diversified angiosperms.  This rich biodiversity has a significant role to balance the ecosystem and has been used ethnologically by locals for various socio-economic and developmental purposes (Yumnam, 2008).

Introduction
Following our comprehensive exploration of scientific crop modeling and the rigorous processes of model assessment, we now pivot to an equally vital, yet distinct, realm of understanding weather in agriculture: Indigenous Technical Knowledge (ITK). This chapter, Indigenous Technical Knowledge for Weather Forecasts and Its Validity,” delves into the profound and often overlooked wisdom embedded within local communities worldwide, knowledge systems accumulated over generations that offer unique insights into anticipating future weather patterns.
In the upcoming sections, we will embark on an in-depth examination of the diverse forms that ITK related to weather forecasting takes. This includes detailed discussions on:
• Observational Indicators: How traditional communities keenly observe subtle changes in the natural world, such as specific patterns in animal behavior (e.g., bird migration patterns, insect activity, animal calls), plant phenology (e.g., early or late flowering, unusual fruiting patterns of wild plants), and the behavior of flora and fauna as harbingers of upcoming weather shifts.
• Atmospheric and Astronomical Signs: The interpretation of sky conditions (e.g., cloud formations, specific colors of sunrise/sunset, halos around the sun or moon), wind patterns, atmospheric pressure changes (often perceived through bodily sensations or changes in local sound travel), and celestial phenomena (e.g., star brightness, moon phases) as indicators for short-term or seasonal forecasts.
• Traditional Ecological Calendars and Proverbs: The role of traditional calendars that map out expected seasonal weather based on historical observations, and folk proverbs or sayings that encapsulate generations of empirical weather wisdom in concise, memorable forms, guiding agricultural practices.

INTRODUCTION

Indigenous Technical Knowledge (ITK) represents the treasure trove of wisdom held by indigenous communities, often overlooked yet immensely valuable. Its impact spans various domains, notably aquaculture, where it has been pivotal. Local fisher communities, drawing from their indigenous knowledge, have been crucial in safeguarding wild fish genetics and improving aquaculture yields. Moreover, their Indigenous and local knowledge (ILK) contributes multifaceted insights to fisheries and aquatic ecosystems science, underscoring the need to preserve and honor this invaluable heritage. Additionally, traditional indigenous systems like aub-anbars have historically demonstrated their effectiveness in practical applications such as passive cooling and water storage, further highlighting the relevance and efficacy of ITK.

Importance of ITK in Aquaculture

Indigenous Technical Knowledge (ITK) is essential in aquaculture, providing valuable methods for pond preparation, water quality management, disease control, and post-harvest operations. These traditional practices, backed by scientific validation, can greatly improve fish production sustainability while being environmentally friendly. Moreover, modern biotechnological tools are increasingly used in aquaculture to enhance fish quality, disease resistance, growth rates, and genetic diversity. Additionally, the concept of intelligent fish farms, employing technologies like IoT and artificial intelligence, aims to optimize aquaculture processes for precise feeding, disease control, and harvesting, promoting green and sustainable practices. Combining ITK with modern biotechnology and intelligent technologies is crucial for advancing aquaculture practices towards efficiency, sustainability, and increased productivity.

7.1. Rice

• Treatment of paddy seeds in diluted bio gas slurry for 12 hours increases resistance of seedlings to pests and diseases.
• During panicle formation in paddy, the flowers of Cycas circinalis are placed on sticks in paddy fields @ 4/ac. Its unpleasant odor repels ear head bugs.
• About 30 kg of tamarind seeds are applied for an acre of paddy field 1 day after transplanting to boost up the crop growth and yield.
• Soaking the paddy seeds in diluted cow’s urine before sowing, considerably reduces the incidence of leaf spot and rice blast
• Presoaking of paddy seeds in milk increases its resistance against ‘tungro’ virus and ‘stunt’ virus
• For control of red leaf spot disease in paddy, the seeds are soaked in ‘Pudina’ leaf extract (Mentha sativa) for 24 hours
• ‘T’ shaped bamboo stands are placed in many places in the paddy fields so that birds can sit on them and feed on the larvae and adults of rice pests.
• Sowing on eighteenth day (Aadipperukku) of Tamil month Aadi (Jul-Aug.) ensures good harvest.
• Daincha (Sesbania spp.) seeds are sown on paddy main fields when paddy nursery is raised and the grown up daincha is ploughed in-situ during field preparation.
• Plough the main field for four to six times for better yield.

The concept of indigenous knowledge or indigenous knowledge system (IKS) delineates a cognitive structure in which theories and perceptions of nature and culture are conceptualized. Thus, it includes definitions, classifications and concepts of the physical, natural, social land economic environments. The dynamics of IKS takes places on two different levels, the cognitive and empirical. On the empirical levels, IKS is visible in institutions, artifacts and technologies.

INTRODUCTION

The fermented products probably came into existence at least before the British Era in Northeastern states of India [before 1824, The Yandabu Agreement between British Govt. and Burma (now Myanmar)] when the British first introduced salt in the region. Interestingly, in all the fermented fish products of this region no salt is used as ingredient which is a major difference with the other fish based fermented fish products of Southeast Asian countries such as Nuac-mum of Vietnam, Budu of Malaysia, Patis of the Philippines etc. Even after the British Era, people used to treat salt as a highly valued and scarce commodity and they used to take an alternative commodity, for example khar in Assam, made from banana plant or papaya. Hence, they could not afford spending salt in fish preservation and they were bound to preserve fish in their own cheaper and easier way.

After attaining a rich experience over a period of time, our forefathers have developed very pertinent primitive knowledge in a wide variety of fields (Rohilla et al., 1999). These location specific wisdom are known as indigenous traditional knowleges (ITKs). Indigenous traditional knowledge are the upshots of the pragmatic approach by our sons of the soil. As such, there are many ITKs emerged due to the incremental and cumulative learning among societies living in close connection with nature. The traditional communities are often called as indigenous or local or ethinic people. They have accumulated lots of empirical knowledge gained from the day-to-day life. The variations in availability of raw materials and environmental conditions clubbed with time tested traditional knowledge and wisdom, have made people of different regions of the North Eastern hill states to formulate, develop and perpetuate consumption of a wide range of traditional foods and beverages unique to its places since ages (Savitri and Bhalla, 2007).

Abstract

Indigenous water saving technologies in arid and semi-arid environment is a local traditional practice for effective use of the limited water supply. These technologies can be used as a base for future development that can be made on these technologies through incorporating the current industry tools. The importance of studying the indigenous knowledge and practices is that it can help to develop enhanced versions of these technologies, and to understand the ability of local communities to adopt the newly developed technology. In Sudan, several indigenous water saving approaches has been used by local societies at various scales. An insight for these technologies, their strength and weaknesses are given here. On “Haffir” (water ponds), Wadi control, and water diversion methods were used for a long time of history for water collection and conservation. At farm scale, terraces, mulching, intercropping, low tillage, fallow, low-water consumption crops and fast maturing crops are common indigenous water saving technologies. Traditional water storage in trees such as Baobab and water-melon is an indigenous proactive along the history in western Sudan to store rain water for long dry season.

Indira Awaas Yojana (IAY)

Indira Awaas Yojana, which used to be a component of Jawahar Rozgar Yojana became an independent Scheme from 1997-1998 onwards. Its objective is construction of free houses to members of the Scheduled Castes/ Scheduled Tribes, Freed Bonded Labourers in Rural areas and also to non SC/ST rural poor living below poverty line. The beneficiaries are selected from the Below Poverty Line (BPL) list approved by the Grama Sabha. Atleast 60% of the total IAY allocation during a financial year should be utilized for construction/ upgradation of dwelling units for SC/ST BPL rural households. A maximum 40% of allocation is for non- SC/ST BPL rural households. 3% of the above categories should be allocated for physically and mentally challenged persons. 

Objective: To quantify aflatoxin content in given sample by Indirect competitive ELIS

Background information:

Enzyme linked immuno sorbent assay (ELISA) is a common biochemical technique that uses the principle of solid-phase enzyme immunoassay (EIA) for detection of substances mainly antigens in a liquid or wet sample.

ELISA is a very popular technique that is used for diagnostic purpose in health sector, plant protection and also for quality test in food and other industries. This technique applies the principle of antigen-antibody reaction on a solid surface. The attached antibody is linked to certain enzymes and in the end of reaction by addition of substrate that performs enzyme-substrate reaction and makes product that has some signal mostly color change and based on intensity of color the qualitative as well as quantitative estimation of antigens can be done using ELISA reader coupled with spectrophotometer.

In ELISA technique one antibody should be specific to certain antigen. For reaction of ELISA the ELISA plates are used that is also known as microtiter plate made-up of polystyrene. The sample to be tested for ELISA contain unknown amount of antigen to be detected, this sample containing antigen is immobiliz d on a solid support like polystyrene microtiter plate in two different ways; first is non-specific where antigens adsorbed to the surface and second is specific where the antigens of sample are captured by specific antibody to the antigen, like used in sandwich ELIS Once the antigens are immobilized non-specifically or specifically the detection antibody should be added that makes precise antigen-antibody complex. The detection antibody may be either covalently linked to certain enzyme or may be detected by another secondary antibody linked to bioconjugated enzyme. During ELISA steps several proteins or antibodies may non-specifically bound that can be removed by washing with mild detergent solution in between of each ELISA step. For washing of ELISA plates, ELISA plate washer may be used that mechanically wash the ELISA plates. At the end of ELISA the final wash is given and substrate is added that produces visible signal in the form of color change that indicates the presence of antigens in the sample and further quantification may be done using ELISA reader coupled with spectrophotometer.

Agarwood Oil

Biological Source : Wood of Aquilaria agalloch, Aquilaria crassna, A. Khasiana.
Family : Thymelaeaceae
Common names : Agarwood, Aloeswood, Eaglewood, Gaharu or Oudh.

Plant Introduction : Aquilaria is a fast-growing, archaic tropical forest tree, which occurs in South and Southeast Asia, from the foothills of the Himalayas to the rainforests of Papua New Guinea. Agarwood is a fast growing, evergreen tree, that normally grows to 18 - 21 m but sometimes up to 40 m in height. It bears sweetly-scented, snow-white flowers. The trees occasionally become infected with a parasite mould and begin to produce an aromatic resin in response to this attack. As the fungus grows, the tree produces a very rich, dark resin within the heartwood. The tree grows in natural forests at an altitude of a few meters above sea level to about 1000 meters, and it grows best around 500 meters. It can grow on a wide range of soils, including poor sandy soil. Seedlings need a lot of shade and water. Trees grow very fast, and start producing flowers and seeds as early as four years old.

Meaning-Steps for individual presentation; Group presentation-Meaning-Stages of group presentation; Impromptu presentation.

A Presentation is the process of presenting a topic to an audience. It is typically a demonstration, introduction, lecture, or speech meant to inform, persuade, inspire, motivate, or to build good will or to present a new idea or product.

Individual Presentations allow a person to completely control the thoughts, ideas, and methods of presenting without outside influence. Individual presentations allow someone to work at their own leisure and pace.

The practice of showing and explaining the content of a topic to an audience or learner is known as presentation. A presentation is a process of offering for consideration or display.

Presentations can also be categorized as expository or persuasive. They can also be impromptu, extemporaneous, written, or memorizes. But it’s more important to focus on their purpose. There are three basic purposes for giving presentations:

Introduction

More and more people are turning to indoor gardening as a fun and functional way to experience nature without leaving the house. A wide variety of plants may be cared for in the comfort of one’s own home by using this method. Indoor gardening may be adjusted to fit any space, whether it a little flat or a large mansion. Improving air quality, adding a supply of fresh herbs, veggies, or colourful flowers, and increasing interior aesthetics are just a few of the many advantages. Regardless of the season or environment, indoor gardeners are able to produce a broad range of plant species thanks to the comfort of regulated temperature and lighting. In addition, tending to houseplants may be a calming hobby that pays dividends in the form of a feeling of achievement and peace of mind.

Growing foliage and flowering potted plants in the home is a great way to develop an intimacy with the nature. Caring and attending the plants gives us immense pleasure and satisfaction, especially retiring after day long working schedule. Now a days, wide variety of houseplants are available that can accommodate almost any indoor location in terms of available space, light and management factors. Research carried at National Aeronautical Space Agency (NASA) has shown that having plants in the home or office, especially in a closed air-conditioned environment, can be good for your health and well-being.

Our indoor environment

It is surprising to know that our comfort zone environment may have 2-5 times the pollution levels than outside air. Although, the magnitude may vary and could potentially reach 100 times depending upon the size of building, occupants, provision of ventilation, volatile organic compounds (VOC’s) emitted from upholstery and paints. The quality of outside air also determine the indoor air quality that may get mixed-up resulting in elevating the levels of carbon-dioxide (CO₂) and other harmful gases beyond the permissible limit. It has been revealed that the indoor CO₂ levels are substantially higher (3-5 times) than those recorded outdoor. The CO₂ being a narcotic cause drowsiness and loss of concentration leading to decline in performance and productivity of employed staff. This sudden upsurge in CO₂ and other toxic gases evolved from the volatile compounds from paints likely raise the Air Quality Index (AQI) that affects the health and working efficiency of building occupants.

It has been scientifically proved that the equipments installed for ventilating closed environment of residential or commercial buildings are not efficient in removing gases and thereby, to some extent filter only the particulate matter (PM). As per the prescribed standards of World Health Organization (WHO) and American society of heating, refrigeration and air conditioning engineers, the maximum permissible limit for indoor CO₂ is 1000 ppm. The term “Sick Building Syndrome” (SBS) is a very common disorder associated now a dayswith the people working in closed environments, especially that lack ventilation. When the CO₂ levels reach 1500 ppm, symptoms such as sore throat, nasal flow and cough are quite commonly observed among the people, thus affecting their general health and well being. Poor indoor air quality has been a major factor linked to SBS, reducing productivity of employees and also cause impaired learning in children in schools.

Introduction

Indian major carps viz. Catla catla, Labeo rohita and Cirrhinus mrigala are the fast growing, consumer preferred and the stay component species in Indian pond aquaculture system. These carps breed spontaneously in natural water viz. river and bundh type tanks where fluviatile condition prevails (Hora, 1945; David 1959; Quasim and Qayyam, 1962; Natarajan, 1971). Above three species under culture condition attain sexual maturity but do not spawn in confined water (Chaudhuri, 1960; Chaudhuri and Singh, 1984). Khan and Jhingran (1975) in their review described several factors viz. temperature, run off water, photoperiod, turbidity and water depth that are playing important roles in initiating natural spawning in ‘bundhs’ and rivers. They further described that no single factor can be considered responsible for spawning separately. The act spawning involves a chain of interrelated conditions as prerequisite to spawn. Monsoon, flood forms the spawning ground and initiates natural spawning. Although fish bred in its natural environment, it was difficult to understand why some species do not breed in confined water where as other species in the same environment breed somoto.

The initial attempt to induce breed IMC (mrigal) was made during 1937. Khan (1938) was able to induce ovulation in Cirrhinus mrigala by administration of mammalian pituitary extract but eggs were not fertilized. No remarkable success on induced breeding of Indian major carps was made till 1957. Indian major carps were induced to breed for the first time on 10th July, 1957 by the injection of aqueous carp pituitary extract at Govt. Fish Farm, Angul of Orissa state by the scientific team from pond culture substation, Cuttack (Chaudhuri and Alikunhi 1957). Eversince 1957, the induced breeding of carps have been tried by many permutation and combination, addition and alteration of inducing agents.

Induced breeding in freshwater fishes is a technique used to stimulate ripe fish to breed in captivity, typically by injecting them with pituitary hormones or synthetic hormone extracts. This method is crucial for aquaculture as many farmed fish species don’t naturally breed in captivity. Induced breeding is a technique by which the economically important fishes (which generally do not breed in captive condition) are breed through artificial stimulation. Induced breeding is a technique whereby ripe fish breeders are stimulated by pituitary hormone or any other synthetic hormone introduction to breed in captive condition. It is also known as hypophysation.
A BRIEF HISTORY OF INDUCED BREEDING
Induced breeding has revolutionized aquaculture by allowing fish to reproduce under controlled conditions, overcoming their dependence on natural environmental cues for spawning. The technique’s development has seen major contributions from various countries, leading to its global application in fish farming.
• 1930 – Argentina (Houssay): The journey began in Argentina with Houssay’s pioneering use of pituitary extract to induce premature birth in livebearing fish. This milestone introduced the possibility of manipulating fish reproduction artificially.
• 1934 – Brazil: Brazilian scientists built upon this innovation by successfully applying pituitary extract for induced breeding, confirming its broader applicability beyond Argentina.
• Expansion to America and Russia: Researchers such as Merlin and Hubs in the U.S. and Gerebilisky in Russia quickly adopted and refined the technique, promoting its international recognition in aquaculture research.
• 1937 – India (Khan): Khan achieved India’s first success with induced breeding in Cirrhinus mrigala. This event marked the beginning of induced breeding in Indian aquaculture practices.

Ketamine and Propofol - “Ketofol”
“Ketofol’’ is a 1:1 (w:w) combination of ketamine and propofol (Taboada and Leece, 2014; Thejasree et al., 2018). The advantages of using both ketamine and propofol in combination (Ketofol) include analgesia, rapid recovery, preservation of airways and maintenance of spontaneous respiration and haemodynamic stability (Saeed, 2011). The admixture has benefits of both drugs, while reducing the side effects of the individual drugs when administered alone (Amornyotin, 2014; Lee and Lee, 2016; Mirfazaelian et al., 2016). Propofol-induced respiratory depression is less with the admixture, while analgesia and cardiorespiratory functions are good due to ketamine (Amornyotin, 2014; Mirfazaelian et al., 2016). Since the adverse effects are dose dependent, the combination helps reduce the dose of the individual drug needed to cause the desired anaesthetic effects. This combination of ketamine and propofol can be used to induce anaesthesia following sedation with any of the previously mentioned balanced sedative cocktails. Induction should be “titrated to effect” based on the depth of sedation achieved from the administration of the balanced sedative cocktails.
Thiopentone and Propofol - “Thio-propofol”
Thio-propofol is a 1:1 vol:vol mixture of 2.5% thiopentone and 1% propofol (Ko et al., 1999; Evans et al., 2002; Larisa et al., 2008). Since the mechanisms of action of propofol and thiopentone are similar, the mixture provides an additive hypnotic interaction (Vinik et al., 1999) and behaves in-between to that of the

ABSTRACT

Holostemma ada-kodien K. Schum, is a laticiferous climber belonging to the family Asclepiadaceae. The root tubers of the plant are medicinally important and are utilized as a major ingredient of the drug, Jivanti. The roots of Holostemma are sweet, ophthalmic, emmolient, alterant, tonic stimulant, aphrodisiac, expectorant and galactagogue and are useful in ophthalmopathy, orchitis, cough, burning sensation, stomachalgia, fever and to cure ‘tridosha’ (Warrier et al., 1995). The sugars and amino acids present in the tuberous roots are responsible for the medicinal properties (Ramiah et al., 1981). There is a huge demand for the root tubers of Holostemma in the South Indian pharmacies and more than 150 metric tons of roots are needed every year (Nair et al., 1992). The destructive and ruthless collection of tuberous roots in recent times has led to acute scarcity of the plant and consequently it has been listed as vulnerable and rare in the FRLHT red list of medicinal plants (FRLHT, 1997). Extending the commercial cultivation of Holostemma is practically impossible due to the increasing land pressure and the perennial nature of the crop. An alternative to meet the increasing demand is to produce the active chemicals in Holostemma through in vitro techniques. In vitro techniques have been extensively studied to produce the different secondary metabolites from various medicinal plants. A strong correlation between secondary metabolite production and morphological differentiation gives more impetus to the application of organized cell culture particularly the root cultures for the large-scale production of phytochemicals in vitro. Transformed root cultures - the so called hairy root cultures obtained after the insertion of T-DNA from the root inducing (Ri) plasmid of the Agrobacterium rhizogenes into the plant genome (Ackermann, 1977) has emerged as an important tool for the production of secondary metabolites in vitro (Rhodes et al., 1987). Hairy roots have advantages of fast growth, organ differentiation and stable secondary metabolite production (Banerjee et al., 1995). We report here the hairy root induction from shoot buds and seedling hypocotyls in infection with A. rhizogenes useful in exploring the possibilities of large-scale in vitro secondary metabolite production in this rare medicinal plant.

ABSTRACT

Trichoderma spp. are important group of fungi which are mycoparasitic on several plant pathogenic fungi. Trichoderma spp. are, therefore, used as commercial biofungicides. Genetic manipulation through induced mutagenesis and molecular cloning are two important tools in improving the biocontrol potential of Trichoderma spp. In the present study, attempts were made to induce genetic variability in Trichoderma virens using UV- induced mutagenesis. In the process, a simple protocol was developed for obtaining high frequency mutations in Trichoderma virens. Using a UV-crosslinker, four stable mutants of T. virens have been developed, which are distinctly different from the wild type in appearance and in antagonism against Rhizoctonia solani and Sclerotium rolfsii. One of the mutants, designated as TI is very slow in growth, but sporulates densely, and retained its mycoparasitic potential against R. solani. Though all the mutants were able to antagonize R. solani, all of them were overgrown by the test pathogen S. rolfsii. These mutants could be used to identify genes involved in growth and conidiation in T. virens.

Resistance to diseases in plants may be enhanced by incorporating resistance genes in susceptible cultivars and this disease management strategy is considered to be the most desirable one. But unavailability of reliable sources of resistance and the requirement of long periods for development of cultivars with built-in resistance have limited the usefulness of this approach. Employing genetic engineering techniques for obtaining resistant cultivars appears to hold promise. However, in the light of expression of concern for the long term effects of genetically modified crops and plant products in several countries, the feasibility of exploiting the genetic engineering methods remains a question mark. In this context, the possibility of inducing natural disease resistance (NDR) mechanisms operating in existing cultivars with high yield potential to provide protection to crops and harvested produce against diseases, has attracted the attention of researchers in several countries. This approach does not involve the introduction of any foreign gene into the plant, but it regulates the expression of defense genes in the susceptible plants. Furthermore this approach is as safe as the use of genetically resistant cultivars for the preservation of environment, since the same mechanisms of resistance are activated in plants with either genetic resistance or induced resistance. Intensive research is being carried out to select effective agents that can be used as inducers of resistance in crop plants to contain various diseases, as alternatives to the development of resistant cultivars through conventional breeding and genetic engineering methods

ABSTRACT

Fruit rot and die back caused by Colletotrichum capsici is the destructive disease in chillies. The potential of Pseudomonas fluorescens for the management of fruit rot of chillies was evaluated under greenhouse and field conditions. Among the various strains of P. fluorescens tested in vitro, the strain Pf1 significantly inhibtied the mycelial growth of C. capsici. Combined application of talc-based powder formulation of P. fluorescens to seed (10 g/kg seed) and foliage (1 kg/ha) effectively controlled the fruit rot disease. Early and high induction of various defense compounds were observed due to P. fluorescens treatment.

ABSTRACT

Vanilla planifolia Andrews - the high value, exotic spice crop has recently gained wide acceptance in the southern and northeastern states of the country. Being an orchid, germination of naked embryo is limited and this favours genetic uniformity. An attempt was made to induce genetic variability in Vanilla through biotechnology tools for its exploitation in crop improvement programmes. In vitro embryo culture and mutagenesis were applied for the purpose. Plantlets were multiplied, hardened, planted out and evaluated for morphological, biochemical and molecular markers. Great interclonal variability was observed in the in vitro derived plantlets. The intraclonal variability was very low in tissue culture derived regenerants. Isozyme and RAPD assay confirmed genotypic variation. Six superior clones were identified and distributed to farmers for further evaluation.

Inductively coupled plasma atomic emission spectroscopy (ICP-AES) uses an inductively coupled plasma to produce excited atoms and ions that emit electromagnetic radiation at wavelengths characteristic of a particular element. A plasma is a neutral gas containing a good number of positive and negative ions or free electrons. A plasma can only be created. A combustion flame is one example of a plasma. Advantages of ICP-AES are excellent limit of detection and linear dynamic range, multi-element capability, low chemical interference and a stable and reproducible signal. Disadvantages are spectral interferences (many emission lines), high cost and operating expense and the fact that samples typically must be in solution.

A new analytical technique called inductively coupled plasma emission spectroscopy has been used for simultaneous multi element analysis of biological materials and soils (Hou and Jones 2000; Wikipedia, 2010; Bradford and Cook, 1997). This technique offers advantages over AAS and other multi-element methods because matrix problems are eliminated or minimized through use of the high temperature argon plasma. Apart from multi- element capability at all concentration levels, plasmas are noted for relative freedom from chemical and ionization interferences that are common with AAS, and detection limits are equal to or better than AAS, depending on the element to be analyzed. Elements such as Al, P, S and B, which are either poorly measured at low concentrations or not possible by AAS, are readily determined with higher sensitivity by ICP.

Intellectual Property (IP) refers to intangible assets that are created through human creativity and innovation. These assets can be legally protected and provide exclusive rights to the creators and owners for a certain period of time.

Importance of IP for Innovators and Entrepreneurs

a) Protecting intellectual property (IP) is essential for fostering innovation and creativity, as it encourages inventors, creators, and entrepreneurs to invest their time, energy, and resources into developing new products, services, and technologies without fear of having their ideas stolen or copied.

For Example, if a software company develops a new program that can revolutionize how people interact with technology, it’s essential to protect the IP of the product by patenting and copyright. This protection ensures that no other company can steal the idea or copy the software, providing an incentive for the software company to invest in the product’s development.

Similarly, if a fashion designer creates a unique design for a dress, they can protect their IP by trademarking the design. This protection provides incentivizes the designer to invest their time and resources into developing new designs, knowing that their creations will be legally protected from copycats.

b) Having intellectual property (IP) protection grants a competitive advantage in the marketplace as it enables creators and owners to have exclusive rights to use, sell, and license their creations without the fear of infringement by others.

Industrial Microbiology is the discipline that uses micro organisms, usually grown on large scale, to produce commercial products or carry out important chemical transformations.

The products derived from such processes are

1 Alcohols and alcoholic beverages.
2 Production of pharmaceutical agents like antibiotics, vaccines etc.
3 Food additives
4 Enzymes
5 Chemicals
6 Single cell proteins

In general, an unwell entity is one that fails to regularly create a surplus and frequently relies on outside funds to exist. The Sick Industrial Enterprises (Special Provision) Act 1985 characterized sickness as “a financial loss over two successive accounting periods and a total loss that is equal to or exceeds the net assets of the enterprise at the conclusion of the second accounting period”.

Revised Definition of Sick Smallscale Industry “A small scale industrial unit is deemed sick if it has incurred losses at the conclusion of the accounting year that are equal to or exceed 50% of the highest level of its net assets in the immediately preceding five f inancial years”.

Symptoms of Sickness

The continuation of many signals for a long time is called a symptom of a disease, and the various symptoms are ultimately reflected in the operation of the facility (capacity utilization rate, financial capital ratio, stock market value practice in various financial areas, production, marketing and labor relations in the industry).

Among the key signs are

• Deterioration of financial relations

• Review delay

• Lack of constant cash flow

• Continued decline in stock prices

• late payment obligations

Considered as one of the leading members of the family Bromeliaceae, pineapple is one of the most extensively consumed fruits in the world. Fruit juices, extracts, concentrates and processed edibles are consumed worldwide. Besides, plant fibres, enzymes obtained from the stems and leaves find many applications across industries. Pineapple residue mainly consists of pulp, stem, leaves and peels and the huge wastes produced as a result of improper handling, harsh environmental conditions, decaying and deterioration. Thesecan be successfully processed and extracted for further inexpensive and sustainable utilization instead of discarding. Agro-based and chemical industries have been switching to using plant-based substrates from various resources. Above all, all the natural substrates obtained are highly renewable, won’t contribute to the global waste production, require less expenditures in processing, less toxic and has considerably better biochemical properties than the synthetic alternatives.

Industrial Importance of Pineapple

Pineapple Leaf Fibre (PALF)

Back in the days, pineapple leaf fibre was considered to be an agricultural residue until it’s potential capabilities as an organic fibre and its commercial prominence heaved into view as a consequence of pioneering researches (Uddin et al.2017). According to Technical Association of Pulp and Paper Industry (TAPPI) standards, many chemical constituents like holocellulose, α-cellulose, lignin, and ash content of PALF were analyzed from a different source of f ibres, with varying environmental conditions, cost, etc. (TAPPI,1991). Mainly because of its highest tensile strength (413 to 1627 MNm−2), high cellulose content, as compared to other organic fibres with its Young’s modulus varying from 34.5 to 82.51 GNm−2. Furthermore, it has also been ascertained that upon its conversion to yarn, a 13% rise in its strength is observed and a bundle of saturated PALF adheres a reduced strength by 50% (Asim et al., 2015). Various researches and aided projects of SITRA (South India Textile Research) under UNDP/UNIDO have demonstrated the production of 100% PALF yarn by blending with other synthetic or natural fibres like cotton or silk. The threads, thus produced, are used to make fabrics, fancy carpets, mops, curtains, etc. where pineapple-jute blended yarns are used by the textile and fabric industries (Mishra et al., 2004; Debnath, 2016). It is also used in furnishings, decorations, sports items, baggage, quilts due to its aesthetic and elegant fabric (Anon, 1992).

Industrial microbiology is an important area of applied microbiology. In a broader sense, industrial microbiology is concerned with all aspects of business that relate to microbiology. It refers to the use of microorganisms in commercial enterprise. Cheap raw materials are converted to valuable products through the metabolism of microbes. Microbes for this purpose could be exploited in different ways.

Various commercial products of economic value made by microbes are :

8.1 Introduction

Contamination of drinking water supplies from industrial waste is a result of various types of industrial processes and disposal practices. Industries that use large amounts of fresh water for processing have the potential to pollute waterways through the discharge of their waste into streams and rivers, or by run-off and seepage of stored wastes into nearby water sources. Other disposal practices, which cause water contamination, include deep well injection and improper disposal of wastes in surface impoundments.

Industrial wastes consists of both organic and inorganic substances. Organic wastes include pesticide residues, solvents and cleaning fluids, dissolved residues from fruits and vegetables, and lignin from pulp and paper to name a few. Effluents can also contain inorganic wastes such as brine salts and metals. This is an incentive for industry to pre-treat their water by neutralizing the chemically active components, recycling, dilution or extraction and collection for proper disposal.

The waste disposal practices, which presently pose a threat to drinking water supplies, include deep well injection of wastes and wastes that are dumped and retained in surface impoundments or evaporation ponds. (Ministry of Industries, 1994).

In keeping with the functions delegated to the Central Pollution Control Board under the Water (Prevention & Control of Pollution) Act, 1974, they covered a number of industries, their production and location with details of receiving water besides details on the raw material they used, the process they adopted to process them, inplant control systems, pollution control system provided and the future requirement with cost implications, monitoring system etc.

These documents are also aimed at developing standards for effluents and emissions as Minimal National Standards that can be adopted uniformly throughout the country. The State Pollution Control Boards (SPCBs) can tighten the standards, wherever the local situation demands.

While implementing these standards, the status of technologies adopted by the industries and further requirements to meet the overall objective of pollution control have been assessed. The technology requirement for pollution control industry wise has been presented in the following groups of industry categories.

Introduction

Digitalization is transforming the digital economy, offering opportunities for entrepreneurs and businesses, and transforming production and trade. The rapid advancement of technology and information systems has led to significant changes in both individuals and companies, particularly in the realm of digitalization in commerce (BarNir et al., 2003). Digitalization uses digital technologies to revolutionize business models, simplify transactions, and enhance value-producing opportunities in processes like customer service, shipping, buying, and selling (Zhang et al., 2013). All over the world, businesses are transitioning from manual to e-commerce for benefits such as efficiency, productivity, security, competitiveness, cost reduction, marketing, promotion, and information distribution (Daniel & Wilson, 2002; Gefen, 2000). E-commerce, a key feature, is reshaping global production and trade (Koh et al., 2017; Scalco, 2017). Moreover, the e-commerce industry has seen a substantial transition over the last few decades, due to rapid technical improvements and shifting customer behaviors.

• Fresh seeds germinate in 20-30 days giving as high as 90% germinate.
• An NPK mixture supplying 375g N + 202 P2O5+375kg o/tree gave the highest mean-field of 33 fruits/tree and 14.88 kg fruit/tree.
• Flowering planting after four years.
• Flower bud differentiation of at 40 leaves stage.
• Flowering time March-April after 7 weeks of fruit development.
• Mealybug attacks shoots and in between the fruit segments – 0.5% phosphamidon or dichlorvos.
• A virus transmitted by lace bug or mealybug causes pineapple wilt.
• Ripon normally can be stored at 5°C for six weeks.
• Suckers weighing 500-750g and slips weighing 300-400g are better for yield and quality.
• Pineapple exhibits vegetative parthenocarpy and self-incompatibility.
• This is non-climacteric fruit.
• Sunscald discord is due to exposure of fruit to sun rays. Multiple crown/ fasciations is another important disorder probably due to genetics/nutritional factors.
• It contains an enzyme known as Bromelin.

Inertial motion is typically excited when the surface currents are impulsively excited by wind forcing. If the wind were to remain steady—then the currents would eventually reach equilibrium and the time-dependent term would go to zero. But if the winds blew for a few hours and then turned off then the current would be still in motion for some time even if there would be no forcing due to turning off winds.

Introduction

Brood stock management is an essential component of fish farming since the success of aquaculture industry mainly depends on quality of brood stock in order to ensure the production of top quality offspring. Their health condition is also important from culture point of view, which in turn increases the fish production. Unfortunately, in India, less attention is given to the brood stock management during non-breeding seasons. The pond aquaculture is also becoming over utilized due to high stocking beyond the carrying capacity, addition of huge quantities of chemicals, drugs, fertilizers and supplementary feeds etc. Physical injury due to rough handling, temperature fluctuations, extremes in oxygen levels can also work in a cumulative way and produce stress and make the fish susceptible to variety of diseases. Environmental deterioration, which occurs due to dumping of pesticides and chemicals alongwith metabolic waste products, left out feed materials and organic load of the pond bottom regularly come in contact with certain vital organs and tissues of fish. All such factors can cause deterioration of fish health and magnify the risk of outbreak of diseases. Disease outbreaks are one of the stumbling blocks in the development of aquaculture industry affecting both the economic development and socio-economic revenue from this sector. Today, there is no availability of exact figure on loss due to diseases in fish farming. The phenomenon is more common in carp Industry, and mortalities due to disease outbreaks cause major management problems and economic loss every year.

Introduction
Infection prevention and control refers to prevention and control of infections and their transmission in healthcare settings. Healthcare Associated Infections (HAI) are one of the most common adverse events in delivery of care and a major public health problem with an impact on morbidity, mortality and quality of life. Up to 7% of patients in developed and 10% in developing countries acquire at least one HAI. These infections also present a significant economic burden for the health system.1It is evident that HAIs result in prolonged hospital stays, long-term disability, increased resistance of microorganisms to antimicrobials, additional cost on health systems, high cost for patients and their family, and preventable deaths.
The core components of IPC2
• IPC programme at the national and health facility level
• National and facility level evidence-based guidelines on IPC
• Education and training
• HAI surveillance
• Multimodal strategies
• Monitoring and audit of IPC practices and feedback
• Workload, staffing and bed occupancy
• Built environment, materials and equipment for IPC
HAI Burden
HAI are one of the most common adverse events in healthcare delivery and a major public health problem with an impact on morbidity, mortality and

This ‘virus’ is widespread in commercial flocks worldwide, but its taxonomic position is not determined. The disease is characterized by aplastic anaemia and generalized lymphoid atrophy with a concomitant immunosuppression. Consequently, infectious anaemia is frequently complicated by viral, bacterial, or fungal infections.

Occurrence

Chickens up to 3 weeks of age are most susceptible.

Synonyms :  Viral bovi. rhinotracheitis, Red nose, I.B.R, Infectious pustular vulvovaginits (IPV), Coital exanthema, Infectious pustular balanoposthitis (IPB), Bovine herpes virus 1(BHV 1).

It is a highly infectious disease of bovine characterized by high fever, rhinotracheitis, conjunctivitis, with a short course and high recovery rate. Encephalitis, the systemic form of the disease in new born calves, infectious pustular vulvovaginits/balanoposthitis are other syndromes caused by the same virus. Genetic isolates of various clinical isolates has found subtypes of BHV 1; a respiratory subtype BHV 1.1, a genital subtype BHV1.2 and an encephalatic subtype BHV 1.3, which is now termed as BHV 5.

IB is an acute, highly contagious, viral disease of chickens characterized by respiratory signs (gasping, sneezing, coughing, and nasal discharge), the severe renal disease associated with neurotropic strains, and a marked decrease in egg production. It is a serious cause of sub-optimal egg production and poor egg quality. This disease is worldwide in ditribution.

Hosts

Chickens only. Recently IBV has been detected in species other than chickens. The disease may be produced only in chickens, IBV may multiply in other species of birds and be a source of infection.

Etiology

IB is caused by the avian coronavirus; numerous strains of infectious bronchitis virus (IBV) exist because of high genetic diversity and cross protection between strains is not reliable.

IBD is an acute, contagious, viral disease of young chickens characterized by diarrhoea, vent pecking, trembling, incoordination, inflammation followed by atrophy of the bursa of Fabricius (BF) and by a variable degree of immunosuppression.

In the USA the disease was reported in Gumboro, Delaware in 1962, hence the name. The occurrence is worldwide.

Etiology

Avibacter paragallinarum Serotype A, B and C. Based on hemagglutinin serotyping, three serotypes (A, B and C) have been recognized.

Epizootiology

The chicken is the natural host although the disease has occasionally been diagnosed in pheasants. All ages of chicken are susceptible but birds up to 10 weeks are less vulnerable.

1. The chronic or healthy carrier birds serve as source of infection. Infection within farm occurs by contact with drinking water or feed contaminated by nasal discharge from sick/carrier chickens. To farms situated at a distance, infection is through air borne transmission.
2. The disease occurs more in cold and wet conditions. The more severe disease occurs when other respiratory diseases are prevalent at the farm.

ILT is an acute viral disease of chickens, pheasants and peafowl characterized by marked dyspnea, coughing, gasping and expectoration of bloody exudate. The disease is worldwide.

Hosts

Chickens particularly susceptible; reported to occur in pheasants and peafowl.

Age Range

All ages susceptible but is most often found in adult (mature or nearly mature) commercial chicken layers.

6.1. INTRODUCTION

All ecologist and biology researchers have to find out: (i) how reliable are the observed results? and (ii) what is the probability of difference between observed and expected results? The question of reliability is solved by estimating the confidence interval and the second question is addressed by the application of statistical tests for significance. Both these methods help us to draw conclusions on our observations, hence, may be grouped together as ‘inferential statistics’.

Referring back to the chapter on sampling, we may recall that it is usually not possible to calculate the ‘population parameter’ directly. Instead, we have to rely on estimates. Two types of estimates can be used, a point estimate and an interval estimate. A point estimate is a single numerical value of a sample statistic that is used to estimate the corresponding population parameter. For example, the sample mean, x , is used as an estimate of the population mean, μ Point estimates are not used widely because the value of some statistic, say mean, varies from sample to sample and we can’ t conclude with any degree of certainty that a single point estimate equals the population parameter. Therefore, an interval estimate is typically favoured. An interval estimate is a range of values within which the population parameter is likely to lie.

8.1. INTRODUCTION

The word ‘non-parametric’ means “not for any specific parameter”. For example, in the student’s t test we use the parameter ‘mean’. In the non-.parametric tests we check the randomness and trend of the pattern of distribution of a variable directly, without using a population parameter for comparing.

In contrast to the parametric tests, where many assumptions are made regarding the distribution of the parameter in the population (i.e. Gaussian features), here we do not make stringent assumptions about the population from which samples are drawn. These techniques are, therefore, also known as ‘distribution free’ tests.

We shall be discussing about 10 such non-parametric tests.

7.1. INTRODUCTION

The word ‘significance’ in statistics does not describe the strength of association. Statistical significance is simply related to the probability of the result being commonly or rarely encountered (If the probability of chance occurrence is low the result is likely to be determined by the set of circumstances under study and not by chance). If conclusions are to be drawn that a difference exists between two groups of subjects, then we have to ensure that they are equal in all respects except the one under test.

‘P’ values are used to assess the degree of dissimilarity between two (or more) sets of measurements. The ‘P’ value is the probability of obtaining a result as extreme as or more extreme than the one observed. The ‘P’ value is actually a measure of surprise and the smaller the value, the more surprising is the result. When ‘P’ value is between 0.05 and 0.01, the result is usually called statistically significant; when it is less than 0.01, it is usually called highly significant and if, values are lower than 0.005, then it is called very highly significant. Traditionally, the approach had been to indicate the level of significance by rounded off ‘P’ values e.g., P < 0.01. Now it has become a fashion to calculate the exact probabilities and thus arrive at a particular decision.

Fertility is the ability of a male to impregnate the fertile female. The reduced or temporary loss of fertility is called infertility whereas permanent loss of fertility is called sterility. A male with low libido or poor sex drive may have excellent semen quality or a male with good libido may have poor seminal quality. So, libido has no direct relation with the male fertility. Male infertility can be categorized into Impotentia coeundi and Impotentia generandi. 1. Impotentia Coeundi • The condition of reduced or lack of sex drive or Inability to copulate (failure of normal service) is referred to as impotentia coeundi. • It may occur due to immaturity, poor experience to perform coitus, inability or unwillingness to mount, inability to perform intromission or failure of ejaculation. • The following conditions may reflect the immaturity or poor/bad experience of the male animal which leads to impotentia coeundi:

Inflammation

Inflammation is a complex process of vascular and cellular alterations that occur in body in response to injury. The term inflammation has been derived from the Latin word inflammare, means to set on fire. Inflammation is considered as an important event in body that activates the existing defense mechanisms in circulating blood to dilute, naturalize or kill the irritant/ causative agent. Thus, it is said that immunity is the resistance of body, while inflammation is the activation of that immunity. It is beneficial to body except when chronic or immune origin. Inflammation starts with sublethal injury and ends with healing.

Etiology

• Any irritant/ injury.

• Bacteria, virus, parasite, fungus etc.

• Trauma. • Physical or chemical injury.