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Garcinia mangostana Linn. (GML) belongs to the family of Guttiferae and is named “the queen of fruits”. It is cultivated in the tropical rainforest of some Southeast Asian nations like Indonesia, Malaysia, Sri Lanka, Philippines, and Thailand. The mangosteen fruit is round in shape, dark-purple, 2 to 3 inches in diameter and contains 4 to 8 segments of snow-white, delicious soft flesh. The fruit may or may not contain seeds. Encasing the flesh is a purple skin 6 to 10 cm thick. Because of its taste and medicinal properties, the mangosteen is often called “The Queen of Fruits”. For centuries, the mangosteen has been honored and cherished by the peoples of Southeast Asia. It is a delicately flavoured fruit of temperate zone.

Mangosteen is native to Malaya Peninsula, and mainly cultivated in Malaysia, Indonesia, Thailand, Sri Lanka, Myanmar, India, Vietnam, Philippines, China, California (USA), Hondurus, Panama, Hawaii, Trinidad and Cuba. In India it is cultivated in lower slopes of Nilgiris, Kerala and West Bengal.

Uses

Mangosteen is mainly used as a fresh fruit. Aril of the seed is edible after being boiled in water. The cortex of the fruit contain tannin, and is used as a commercial dye. The rind of fruit is astringent, which is used in chronic dysentery and diarrhea. The bark and young leaves are used to gargle for sore throat. It contains a crystalizable substance known is mangosteen.

Mangosteen named after the French priest and explorer Laurentiers Garcin is thought to have originated in Southeast Asia. It was said to be Queen Victoria’s favourite fruit. The correlation of Queen Victoria with the mangosteen is probably related to the fact that numerous people have referred to it as the “Queen of Fruits”. It is one of the most praised tropical fruits and certainly the most esteemed fruit. Most people enjoy mangosteen and the fruit now categorized as a “super fruit” has a ready market in western countries where it is considered a tropical delicacy. Mangosteen is primarily consumed as a fresh fruit.

Genus : Garcinia
Species : mangostana L.
Family : Guttiferae
Chromosome No. : 2n = 56-76, 88-96, 120-130

In family Guttiferae to which mangosteen belongs, very few plants are fruits and yield drugs, gums and resins. It is a tropical fruit and occupies an important place in Garcinia family. The European voyagers on their return indicated wonderful stories of the East and mention of mangosteen received huge praise. Hacobus Bontius termed the fruit as ‘Queen of fruits’ the finest fruit in the world. Because of beautiful colouring of fruit alongwith pleasant delicate flavour makes mangosteen the most esteemed fruit of Asiatic tropics and no other tropical fruit equals to that of mangosteen. Despite being designated as the finest fruit of the tropics, the fruit is mainly confined to the limits of East Indies. The species is found wild in the forests of Sunda Islands and of the Malay peninsula. Writers have indicated that mangosteen is usual dooryard tree in the East Indies, more in Java and Sumatra. Some small orchards of mangosteen have come up in Sri Lanka, but they are most abundant only in Malay Archipelago.

Abstract

Microorganisms are potential tool for the production of large amount of potent and effective health care, food products and biofertilising agents. Recently, the marine microorganisms are considered as good sources of such products which may possess novel properties. There is a high scope for the exploration of marine microbes in order to search new product of economic importance from marine resources. Mangrove habitat is also part of marine habitat with the plant community having unique adaptation in their morpho- physiological and reproductive behavior. The high level of organic content in mangrove rhizosphre also leads to the colonization of degradative microorganisms and make them responsible for the creation of diverse and potential microbial community. Hence, mangroves are also considered as untapped resources of microbial metabolites and products. Several microorganisms including bacteria, fungi, algae, cyanobacteria, lichens are documented from mangrove habitats. These inhabitant microbes are the good source of secondary metabolites, therapeutic enzymes, food protective agents and mineral solubilisers. However, information on these aspects is very meager and thus the present review is an attempt to compile informations on the sources, properties and application of important microbes from mangrove ecosystems.

Origin and diversification of Mangroves

Mangroves are quite old, possibly arising just after the first angiosperms (Duke 1992). Based on the fossil evidence and recent molecular studies, it is believed that mangroves originated along the shorelines of ancient Tethys Sea and there was a dispersal along the Tethys seaway westward into the Atlantic and further into the east Pacific (long before the closure of the Panama Isthmus) and eastward into South east Asia and Australia during the Late Cretaceous or early Eocene around 80 million years ago (Duke 2006). Nypa seems to be earliest fossil evidence. However, prior to closure of Tethys sea, around 40-55 million years ago, the mangrove genera - Nypa, Acrostichum, Rhizophora, Avicennia, Pelliciera, Sonneratia, Bruguiera, Ceriops, Heritiera, and Aegiceras - were present around shoreline (Duke 2017). The closure of Tethys Seaway by the mid-Tertiary (34-50 Million years ago) by northward movement of African landmass, created a physical barrier that completely terminated the Atlantic East Pacific (AEP) and Indo-West Pacific (IWP) exchange route, and resulted in subsequent diversifications within the AEP and IWP regions influenced by region-specific events (Ellison et al. 1999; Duke 2006). The plate tectonics and other vicariant events like Pleistocene sea level rise are attributed to the current distribution pattern and disjunct distributions of species within genera. Duke (2017) hypothesised two separate westward dispersal routes in AEP: (i) a southern path along the coasts of West Africa, South America and, (ii) a northern path along the North American east coast, whereas he hypothesised two isolated pathways in IWP towards eastward dispersal: (i) a northern path along the coast to the Middle East, Southeast Asia and, (ii) a southern path along the coasts of East Africa, India, and Australia. Further he noted that collision of India and Australia with Middle East and Southeast Asia respectively, resulted complication in dispersal route. Based on the available records it is presumed that diversification of mangrove plants has largely been driven by three key processes of speciation viz., Allopatric speciation, Peripatric speciation and Parapatric speciation (Duke 2017).

Mangroves Definition

A mangrove is tree, shrub, palm or ground fern, generally exceeding one and a half meter in height that normally grows above mean sea level in the intertidal zone of marine coastal environment and estuarine regions of the tropical and subtropical coastlines (Duke 1992). The mangrove is also defined as assemblages of salt tolerant trees and shrubs. Mangroves are taxonomically heterogenous and more related to environment than terrestrial or aquatic plants.

The term ‘mangroves’ is used to define both a group of plants and a community. To avoid confusion, Macnae (1968) proposed the term ‘mangal’ for the mangrove forest community, and the term ‘mangroves’ for the plants. Other terms for the mangrove forest community are coastal woodland, oceanic rainforest, intertidal forest, tidal forest, blue carbon forest, mangrove forest, and mangrove swamp.

Generally, mangrove species are grouped into two types: Exclusive and Non-Exclusive. The exclusive species are limited to the mangrove habitat, and they are also referred to as strict mangroves, obligate mangroves or true mangroves. The non-exclusive species are mainly distributed in a terrestrial or aquatic habitat, but they also occur in the mangrove habitats and they are referred to as semi-mangroves, back mangroves or mangrove associates. Tansley and Fristch (1905) are the first to introduce the criteria to classify mangrove species into true mangroves and mangrove associates. Tomlinson (1986) used rigid criteria to distinguish true mangroves from mangrove associates. In his criteria, true mangroves possess all or most of the following features:

Distribution of Mangroves in India

Mangrove forests in India are found along the coastline of 9 States and 4 Union Territories (Fig. 5). The mangrove habitats are broadly classified into three namely, Deltaic (Eastern Coast Mangroves), Estuarine & Backwater (Western Coast Mangroves) and Insular mangroves (A & N Islands) (Mandal and Naskar 2008). India has a total mangrove cover of 4975 km²; of which, 57% is present along the east coast (Bay of Bengal); 31% along the west coast (Arabian Sea) and the remaining 12% in the Andaman and Nicobar Islands (FSI 2019).

Basic Requirements

Hybrid seeds could be produced at cheap rates only if the crop species have certain built-in morphological or physiological mechanisms, suitable for the control of parentage for successful hybrid seed production and its floral morphology, is amenable to it. Some of the basic requirements and techniques employed in large-scale commercial hybrid seed production of crop plants are as follows:

• Availability of a proven heterotic hybrid combinations which could distinctly and profitably surpass the yield levels of commercial variety being grown.
• Availability of an easy, economic and effective means of eliminating or rendering functionless of male part of bisexual seed parent, mechanically, genetically or even biochemically.
• Availability of a strong fertility restoration system (in case of the use of cytoplasmically governed male sterility system) or availability of a tightly linked marker gene system in genetically governed male sterility along with full and detectable expression of self-incompatibility.
• Absence of modifier genes or gene systems in case of the use of self-incompatibility for hybrid seed production.
• Complete synchronization of flowering in both seed and pollen parents.
• Free, unrestricted and natural transfer of pollen from pollen to seed parents.
• Good seed setting on seed parent.
• A skilled organized effort for large-scale seed production, certification, processing and well-knitted distribution of hybrid seeds.

Manila tamarind (Pithecellobium dulce  (Roxb.) Benth.), also known as ‘Jangle jalebi’, ‘Madras thorn’, Sweet inga and ‘Monkey pod’ has a potential to become an important tree of semi- arid and arid regions since it is a drought hardy plant and tolerates harsh environmental conditions as well as salinity. In general, it is planted on the boundary of orchard, road and field sides as protective hedge plant. It is a medium size, much branched tree, suitable for silvi-pastoral system (Gupta et al., 1994). It is drought resistant species but susceptible to frost under arid conditions, coming up well in areas of low rainfall due to its extensive root system. Names like dulce (sweet) and “Manila tamarind” reflect the wide use of the pods as food. The most valuable and commonly used part of Manila tamarind tree is the pulp, which is white or red, very sweet and has a pleasant flavour. The large aril represents 50 per cent of the fruit (Seeds 25%, husk 25%). The major constraint in commercialization of this tree species is lack of improved genotypes, agro-techniques for cultivation in different ago-climatic conditions and limited commercial value.

Abstract

Among the different hand thinning treatments, thinning of lateral flower buds resulted in maximum yield of ‘A’ grade fruits (42.30 kg/vine) without affecting the total yield (86.34 kg/vine). The fruit size and weight was significantly increased with increase in the intensity of thinning. The maximum fruit size (length and breadth) and weight were recorded when only two flower buds or flowers, or fruitlets/fruiting shoot were retained, but the total yield was significantly reduced as compared to other treatments. In the second experiment, hand thinning treatment was integrated with the plant growth regulators i.e. CPPU and cytokinin. The results revealed that 5 ppm CPPU in combination with hand thinning (thinning of lateral flower buds to 2 flower buds/fruiting shoot) significantly increased fruit size, yield and percentage of ‘A’ grade fruits. This treatment produced 75 per cent ‘A’ grade fruits as compared to only 19 per cent under control.

In the coffee family Rubiaceae, Rubia cordifolia L. (RC) is a useful medicinal plant in the Ayurvedic system due to its many pharmacological characteristics. It is also known as common Madder or Indian Madder. It was grown for a red pigment generated from the roots. Rubia was divided into roughly 70 species that were found all over the world, including 36 species and two variants described from China. Because of their high bioactivities, Rubia plant extracts and phytochemicals have gotten a lot of interest.

Morphological characteristics

It is a perennial, prickly climber with a stem, growing up to 12 m long. Leaves are highly variable, ovate lanceolate, 5-7 nerved, 2-10 cm long and 2-5 cm broad, occurring in whorls of 4-6. The stems are slender and quadrangular. The leaves are simple, opposite and stipulate, the stipule is interpetiolar. The petiole is 1.5–3 cm long. The blade is lanceolate, 1.5–5 cm × 0.5–2.5 cm, cordate at base, obtuse acuminate at apex and displays three to five longitudinal nerves. Roots are perennial, long, cylindrical, and rusty brown in colour

Importance and Value of Human Resources

The employees are the human resources of an organization which are crucial for its functioning. The acquisition of human resources and their maintenance involves costs. Manpower costs include costs of research and designing of manpower programmes that is man-power planning, work study and designing of personnel policies etc. The costs of commissioning of man power, that is, costs of recruitment, induction, placement and training etc. The cost of maintenance of these resources is a very significant component of the total costs of an organization. Maintenance costs include wages and salaries, welfare and medical benefits, PF, gratuity and other retiring benefits etc. These costs form a substantial part of the total costs of an organization. In the last 30 years the treatment of manpower costs has been recognized as a separate division in accounting practices. The other assets like machinery, buildings etc come under depreciation. In contrast, human assets are the employees at all levels, as they grow in age and experience, add to their productivity and capacity to earn for the organization and become more valuable.

Soil fertility and crop production can be maintained only by efficient and judicious management of nutrients addition to the soil from external sources. Of the two sources most widely used all over the world, one is organic in nature- the organic manures, simply called manures, and the other comprises the synthetic or naturally occurring chemical fertilizers, simply called fertilizers. A brief account of manures is described hereunder:

The word manure came from Middle English manuren meaning to cultivate land, and initially, from French word manoeuvre meaning hand work alluding to the work which involved manuring land. The term manure originally meant that which was worked by hand (Fr. manoeuvre), but gradually, came to apply to any process by which the soil peroperties could be improved. Prominent among such processes was that of directly applying manure to the land, manure in this sense being what is now called farmyard manure or dung, the excreta of farm animals mixed with straw or other litter. Ronald Fisher seems to have used the word manure systematically for what we would call fertilizer today.

Uses of Manure

Manure has been used for centuries as a fertilizer for farming since it is rich in nitrogen and other nutrients, which facilitate growth of the plants. Liquid manure from pig or hog operations is usually knifed (injected) directly into the soil to reduce the unpleasant odours. Manure prepared from hogs and cattle is spread on fields using a manure spreader. Due to the relatively lower level of proteins in grasses, which herbivores eat, cattle manure has a milder smell than the dung of carnivores, e.g., elephant dung is practically odourless. However, due to the quantity of manure applied to fields, odour can be a problem in some agricultural regions. Poultry droppings are harmful to plants when fresh but after a period of composting are valuable fertilizers.

The dried manure of animals has been used as fuel throughout history. Dried manure (usually known as dung) of cow was and still is an important fuel source in countries such as India, while camel dung may be used in treeless regions such as deserts.

Introduction Livestock plays a vital role in rural economy. Apart from milk, which is a significant economic contributor, efficient use of livestock resources and byproducts like Manure/dung in biogas systems can help generate localised clean cooking energy for rural masses. At the same time, biogas spent slurry; a by-product of biogas plant is a potential source of major and micronutrients besides organic matter. Its proper utilisation after processing has a vast potential in meeting the nutrient requirement of the crops and thereby reducing use of chemical fertilizers besides providing sustenance to soil health. It is estimated that about 443.5 lakh tonnes of solid bio-slurry could be made available from dairy animals. This bio slurry can be processed to produce bio fertilizers which can meet the India’s requirement of NPK and micronutrients (Fe, Mn, Zn, Cu) by about 4.5% and 0.4% respectively. Vermicomposting is the best technique to convert livestock waste into a resource. In India livestock manure is managed basically in three ways:

A. Majority cases dung/manure excreted by livestock are removed and dumped into heaps nearby cattle sheds. The heaps remained for some time when they get converted into manures, which are spread subsequently in the fields as an organic matter.

B. Manure/dung is utilized for energy purpose in village level where the dung is made into small cakes and dried and later on used as a fuel for cooking purposes. This is a great wastage of organic matter which needs to be utilized or managed in other ways so that both the energy and manure can be obtained from the livestock waste.

C. This method of manure utilization is popularized both by central and state government through bio-gas plant where manure is used for the production of methane under anaerobic (lack of oxygen) conditions. The methane gas is used for cooking purpose as well as the slurry after methane extraction is used as farm manure. This management and technique reduce methane emission in environment utilized the methane for cooking purpose and at the same time the valuable waste is utilized as a farm manure.

Growth is the development of a plant as a whole or of a specific organ. Besides the genetic factors, the environmental factors grouped as climatic factors and soil factors influence plant growth. The supply of mineral nutrient elements to the plants is discussed in this chapter.

A complete analysis of plants detects large number of elements. But only certain elements are essential. An element is said to be essential if the plant cannot complete its life cycle without it, and if the malady (deficiency) that develops in plants in its absence can be remedied only by that element.

Earlier 16 elements were considered as essential for plant growth. They are carbon, hydrogen, oxygen, nitrogen phosphorus, potassium, calcium, magnesium, sulphur, iron, manganese, zinc, copper, molybdenum, boron and chlorine. Recently, sodium, cobalt, vanadium, silica, selenium, gallium, aluminium and iodine are added to the above list. One or the other of these elements (recently added) has been found to be essential for a particular species of plants.

Carbon dioxide, water and molecular oxygen are the forms in which C., H and O are assimilated by plants. Others are taken up by plants from the soil. Nutrient uptake by plants accounts for about 10 percent of total dry weight of crops, the remaining percentage being water. The chemical symbol and the ionic forms in which the essential elements are absorbed by the plants are as follows.

Manures are the plant and animal wastes that are used as source of plant nutrients. They release nutrients upon their decomposition.

Bulky organic manures contain small percentage of nutrients and they are applied in large quantities. e.g,. farmyard manure, compost, green manures.

Introduction

Manures are obtained from the plant residues, green manure, compost and animal wastes that are used as source of plant nutrients. They release nutrients after their decomposition. They essentially function as sustenance to the crops. They help in the proper cultivation of crops. The addition of manure enables the soil to hold more water. Organic manures have acids that dissolve the nutrients in the soil and make them available to the plants. manures can be grouped into bulky organic manures and concentrated organic manures. Brief dissection about manure given blow flow chart.

Bulky Organic Manure

Bulky organic manures are those materials of plant and animal origin, which contain small percentage of nutrients when added to the soil have tendency to decrease bulk density and to increase soil volume, thus providing better physical conditions for plant growth especially in coarse textured soils and also provide essential nutrients in smaller quantities than the chemical fertilizers. Characteristics of bulky organic manures:

Plants requires a number of nutrients for their growth and reproduction, of which six are primary in nature and they are,

1. Carbon - Plants are taking carbon from the air and soil
2. Hydrogen from water
3. Oxygen from water and air
4. Nitrogen from soil and it helps plants to synthesize protein for their growth they need to produce new tissues. Nitrogen is often in shortage in soil for the plants, and plants will try to take as much available from the soil at the same time too much of nitrogen will help the foliage growth and but not the setting of flowers and fruits.
5. Phosphorus stimulates root growth and helps the plants set flowers and seeds. Organic matter and activity of soil organisms increases the availability of phosphorus.

The word “Manure” is originated from the French word “Manoeuvrer” which refers to “work with soil”. The word Manure is also originate from Latin word “Manu” (hand) and operate (to work). Manures are the substances which are organic in nature, capable of supplying plant nutrients in available form, bulky in nature having low analytical value and having no definite composition and most of them are obtained from animal and plant waste products. Formerly, the word in use was “Dung ”in English (at present fertilizer).

Introduction

Fish and the aquaculture products contribute a significant amount of animal protein to the diet of the people worldwide. Being highly nutritive they serve as valuable food supplement in diets lacking essential vitamins and minerals. Besides its role of food security, fish is a source of income for millions of people around the World. Though the trade is not prominent at Global level, in some countries its contribution to foreign currency earning is vital to the National Economy. India has a coastline measuring 7500 km and important aquaculture country in the World. Being the home for more than 10% of the global fish biodiversity, the country ranks third in the World in the total fish production with an annual fish production of about 6.4 million tones. Constituting over the 1% of the GDP, fisheries contributes to 5.3% of the agricultural GDP. The estimated foreign earning for the year 2006 was US$ 1365 million.

ABSTRACT

The purpose of this paper is to map the diversity of Formicoidea, richness and habitat preferences with respective to the different forest cover, classified based on the NDVI from LANDSAT TM imagery with the help of remote sensing and GIS. The study is carried out in Sirumalai hills region of Dindigul District, Tamil Nadu state, India recorded a total of 26 species with 15 genera belonging to four sub-families by a taxonomical basis. The NDVI based forest classification results that the area has disturbed dry deciduous forest (SA), undisturbed dry deciduous forest (SB), disturbed mixed deciduous forest (SC), undisturbed semi evergreen forest (SD), undisturbed grassland (SE) and undisturbed dry evergreen forest (SF) types of forest covers. The results of diversity studies, statistical analyses and field study reveals that the tropical semi evergreen forest and tropical dry evergreen forest have yielded more number of species, genera than the mixed deciduous forest and dry deciduous forest which are most disturbed by human beings in establishment of estates, management strategies by the government and land use by the local farmers.

ABSTRACT

Mapping and exploration of iron ore deposits in parts of Salem District is carried out based on the detailed field work and the interpretations of IRS-1C PAN data in the scale of 1:12,500 and aerial photographs (NRSA, Scale 1:4,000). The study results that the major rock types of the study area are banded magnetite quartzite, garnetiferous pyroxene granulite, amphibolite, garnetiferous pyroxenite, charnokite, talc tremolite, actionalite and dunite rocks. The Banded Magnetite Quartzite’s (BMQ) occur as enclaves within the hornblende gneisses at several parts of the study area. Topographically, the rocks constitute small low-lying linear ridges at some places it is found to be closely associated with garnet pyroxene granulites. A remarkable feature about the mode of occurrence of magnetite quartzite is its linear nature and the physical form, which could be described as a “Band”. All the bands possess an ENE-WSW strike and the dip is found to be steep (above 70 degrees) towards north. The rock in the hills of Godumalai, Vellalagundam, and Singipuram are highly deformed and contact with garnetiferous pyroxene granulite and hornblende gneisses. In hand specimen, the magnetite quartzite rock exhibits fine banding. In general no variation in grain size or thickness of banding could be seen megascopically. However, occasional increase in size of magnetite might be possible as seen from the coarser magnetite pebbles strewn in the channels of the area. There are two types of folds observed namely F1 and F2. The axial plane trace of the F1 varies in trend from WNW-ESE to WSW – ENE. The joints are traced in the directions of NNW-SSE to NNE – SSW and WNW – ESE to ENE-WSW. The interpretation of lineaments shows their trend directions of NNE-SSW, NE-SWand NNW-SSE. The above said work has carried out through detailed field work and interpretations using by IRS-1C PAN data in the scale of 1:12,500 and aerial photographs of NRSA, in the scale of 1:4,000 for the study area.

ABSTRACT

With the increasing use of ground water for various activities, the fast decline in groundwater takes place. In order to prevent the aquifers from fast depletion, the ground water recharge is necessary. This study attempts to identify the favourable areas for groundwater recharge and suggest suitable recharge structures toaugment the aquifer system of the study area using Remote Sensing and GIS. The various thematic maps such as Geomorphology, Geology and lineaments, Soil, Slope, Land use, Drainage, Runoff, Depth to weathered zone, Groundwater level fluctuation and Water quality are used in the analysis. The favourable areas for groundwater recharge have been categorized into highly favourable, moderately favourable, less favourable and poor. To delineate areas favourable for recharge zone various themes are interpreted. The identification of ground water recharge zones for the study area is useful for improvement of water quality, quantity and better augmentation & management of Groundwater recharge.

Soil map is a map showing diversity of soil types and its properties such as pH, texture, organic matter and depth of horizons in the area of interest obtained as the end of soil survey. Generally, soil maps are used to simply identify soils and their properties, but are sometimes required for more specific purposes, such as the suitability of soils for a particular crop, suitability for irrigation, erosion risk and many other specific needs or environmental threats.

Soils fulfil many functions important for agriculture, forestry and the management of soil resources and natural hazards. The functionality of soils depends on their properties; hence, accurate and spatially highly resolved maps of basic soil properties such as texture, organic carbon content and pH for specific soil depth are needed for the sustainable management of soils. The surface soil organic carbon (SOC) concentration is a useful soil property to map soils, interpret soil properties, and guide fertilizer and agricultural chemical applications.

Traditionally farmers apply fertilizers, chemicals and other crop production inputs to optimize the production of the field as a whole. This results in application of over application of inputs and low inputs in certain locations of the field because of variation in field characteristics including soil organic carbon, soil texture, soil nutrients, field topography, and other properties. In addition, uniform applications may increase the chances of pollution of the environment due to excess application in some field area. Precision farming technology has been shown to optimize application rates if the variation of field characteristics can be used to guide the application rate of crop-production inputs. The organic carbon concentrations of surface soil have been used to spatially vary the application rate of some crop-production inputs. The surface soil organic carbon concentration affects the activity of many herbicides, influences plant-available nitrogen and also affects the soil’s ability to adsorb plant nutrients. Knowing its concentration may therefore be useful, especially if its spatial distribution could be determined accurately and for low cost.

Genetic Mapping

Perhaps one of the most powerful applications of markers is genetic mapping. This technology allows researchers identify markers located within or very close to genes of interest. With this it is possible to trace these genes through generations of crosses.

If the trait in question is complex and multigenic, mapping will also be of tremendous help to dissect the various genes or loci that contribute to the overall characteristic. Determining the amount of impact each locus delivers is a process called Quantitative Trait Loci or QTL mapping.

1. Introduction

Electronic theses and dissertations (ETDs) are a great resource for scholars in academic institutions. They assist academics in comprehending the study carried out in prior years. When study results are made accessible on an open access platform, they are extensively utilized for further inquiry and teaching purposes. Theses and dissertations need to be made publicly available so that the users can get access to it. So, the emergence of Shodhganga proves to be very useful to the students, researchers, faculties and all other users (Vijayakumar and Vijayakumar, 2007). A significant number of doctorate research projects are now being conducted worldwide in several fields within the natural sciences. Bibliometrics focusses on analyzing the attributes of published literature, such as the distribution of literature on different subjects, statistical examination of content kinds, references, citations, and co-citations within and across fields (Pritchard, 1969). Davarpanah and Aslekia (2008) conducted bibliometric research on PhD theses, however these investigations were often limited to a certain university or department. Shodhganga serves as a digital archive for freely accessible whole texts of Indian electronic theses.

Marburg virus (MV) disease was named after the West German town where the largest outbreaks of human infection was reported. It is also known as Vervet monkey or green monkey disease as monkey species introduced the disease in Europe. Marburg disease virus was reported in 1967 in Frankfurt-on-Main and Marburg, West Germany and Belgrade, Serbia in vaccine factory workers who came in contact with blood, organs or cell cultures of African green monkeys. Ebola and Marburg hemorrhagic fevers are severe zoonotic diseases seen not only in humans but also in non-human primates. Most species of Ebolavirus and the only known species of Marburg virus occur in Africa. Primates are infected sporadically from an unknown source probably bats. Humans seem to become infected directly from bats in caves, as well as when they, come in contact with tissues from infected apes and other species. Once the virus has entered the population, it can spread from person to person. Some epidemics affect hundreds of people. Although the mortality rate varies, 90% case fatalities have been reported in the most pathogenic viruses. No vaccine is available and the only treatment is supportive. Epizootics in gorillas and chimpanzees are equally serious, and appear to threaten the survival of these species in the wild. Other wild mammals including duikers also seem to be killed during outbreaks. One species of Ebolavirus, Reston Ebolavirus, occurs in the Philippines. Until 2008, this virus was known only as an infection of nonhuman primates. It does not appear to be a human pathogen; although some people in contact with this virus have seroconverted, they remained asymptomatic. In December 2008, Reston ebolavirus was discovered in pigs during an unusually severe outbreak of porcine reproductive and respiratory syndrome (PRRS) in the Philippines.

Etiology: Ebola and Marburg hemorrhagic fever are caused by members of the genera Ebolavirus and Marburgvirus, respectively. These viruses are the only members of the family Filoviridae. The genus Ebolavirus contains four recognized species: Zaire ebolavirus (formerly Zaire Ebola virus), Sudan ebolavirus (formerly Sudan Ebola virus), Ivory Coast ebolavirus (formerly Cote d'Ivoire Ebola virus) and Reston ebolavirus (formerly Reston Ebola virus). At least 13 strains of these viruses have been identified. A fifth species, tentatively named Bundibugyo ebolavirus, was isolated from a recent outbreak in Uganda. Marburg virus contains a single species, Lake Victoria Marburgvirus (formerly Marburg virus). Six strains of Lake Victoria Marburgvirus had been recognized as of 1990; at least nine genetically distinct strains were identified from a more recent outbreak in the Democratic Republic of the Congo. Marburg virus contains ssRNA as genome. The virion is highly pleomorphic and measures 80 nm in diameter and 700-1000 nm in length. Filoviruses can be destroyed by autoclaving. Treatment with sodium hypochlorite (1:100 dilution of household bleach) or standard hospital disinfectants can also be used. Ebolaviruses are reported to be susceptible to 2% sodium hypochlorite, 2% glutaraldehyde, 5% peracetic acid and 1% formalin. These viruses can also be inactivated by ultraviolet light, gamma irradiation, 0.3% betapropiolactone for 30 minutes at 37°C (98.6°F), or heating to 60°C (140°F) for 1 hour. Marburgvirus is susceptible to 1% sodium hypochlorite, 2% glutaraldehyde or formaldehyde, ultraviolet light and heat.

Abstract

The unique characteristics of mare’s milk help to prevent many chronic diseases. The health beneficial properties of this milk is even acknowledged since ancient time and this milk is being successfully used as medicated food against a wide range of chronic diseases. The merits of mare’s milk has also been noted by the cosmetic and pharmacy industries. Lastly, considering many health beneficial effects of this milk, attempt should be made to augment the production and popularization of mare’s milk. In this chapter different aspects of mare’s milk has been pointed out in different sections and subsections.

Introduction

Globally, 16.9% of milk consumed by humans comes from species other than cattle. Non-cattle milk is linked more to territories than cows’ milk: sheep in the Mediterranean basin, horse in Central Asia, yak in Himalayas, camel in desert regions. The domestic equid species (horse and donkey) belong to the taxonomic order Perissodactyla, family Equidae and genus Equus. The nutritional and therapeutic properties of horse (Equus caballus) milk have been known since ancient times according to the historian Erodoto (V century BC). Although horses are of minor importance for milk production in comparison with cows, buffalo, sheep and goats, they have been traditionally important dairy animals in Mongolia and in the southern states of the former Soviet Union, e.g., Kazakhstan, Kyrgyzstan and Tajikistan. Aristotle (322 BC) reported the existence of a dairy product in Mongolia that processed mare’s milk to transform it into a fermented product with an alcohol content of 3% and Herodotus also reported something very similar performed by the Scythians;

Marek’s disease is a herpesvirus-induced neoplastic disease of chickens characterized by infiltration of various nerve trunks and/or organs with pleomorphic lymphoid cells. Marek’s disease is world wide in distribution. Marek’s Disease has been named after Josef Marek who published the disease in 4 roosters in 1907.

Occurrence

In chickens only, however turkeys and quail have limited susceptibility. Lesions similar to MD have been seen in pheasants, ducks, pigeons, geese, canaries, budgerigars, swans and great horned owls. However, aetiology for lesions in these species has not been proved. It may appear at 3-4 weeks but usually after eight weeks, with major losses from 12-25 weeks. Morbidity and mortality varies depending on the virulence of MDV.

In India marigold is one of the most commonly grown flowers and used extensively on religious and social functions in different forms. Because of their ease in cultivation, wide adaptability to varying soil and climatic conditions, long duration of flowering and attractively coloured flowers of excellent keeping quality, the marigolds have become one of the most popular flowers in our country. Flowers are sold in the market as loose or as garlands. Due to its variable height and colour marigold is especially use for decoration and included in landscape plans.

Bud caterpillars : Helicoverpa armigera : Noctuidae

Eggs are laid singly on young buds.  Larvae feed on developing flowers by damaging florets. They can also eat the leaves.

Leaf webber : Phycita sp. : Phycitidae

Larvae of Phycita sp. feed on heads of buds and flowers.

Marigold is one of the easiest annual flowers to cultivate, having wide adaptability. Flowers are attractive, have good keeping quality with a wide spectrum of colour, shape and size. They produce marketable flowers in a short time and are used widely for religious and social functions as well as for garden display. 

Importance and Uses

 Marigolds are sold as loose flowers, as cut flowers and widely used for various decorations and garland making. They are also excellent landscape plants widely used for bedding purpose, in herbaceous borders and can also be grown in pots. French marigolds, being dwarf, are suitable for hanging baskets, rockeries, edging and window boxes. In fact, marigolds rank next only to jasmine in popularity in South India 

Marigold (Tagetes spp.) an annual flowering plant belongs to family Asteraceae, alternately known as Compositae. The word “marigold” means “Mary’s Gold” and refers to the “Virgin Mary”. However, word “Tagetes” named after the Etruscan God “Tages” who supposedly emerged from the earth as it was being ploughed and was imbued with the power of divination. Cultivated species include Tagetes erecta commonly referred as African marigold and Tagetes patula as French marigold. Marigold commonly propagated by seed and cutting is a leading loose flower of India. Selection of variety is favoured by a higher proportion of double inflorescences.

Marigold (Tagetes spp.) an annual flowering plant belongs to family Asteraceae, alternately known as Compositae. The word “marigold” means “Mary’s Gold” and refers to the “Virgin Mary”. However, word “Tagetes” named after the Etruscan God “Tages” who supposedly emerged from the earth as it was being ploughed and was imbued with the power of divination. Cultivated species include Tagetes erecta commonly referred as African marigold and Tagetes patula as French marigold. Marigold commonly propagated by seed and cutting is a leading loose flower of India. Selection of variety is favoured by a higher proportion of double inflorescences.

ORIGIN AND DISTRIBUTION

Origin of marigold is central and South America especially Mexico (Kaplan, 1960). Bailey (1951, 1963 and 1976) reported that marigold is a native to Arizona and Mexico to Argentina.

During early 16^th century it spread to various parts of world from Mexico. African marigold (Tagetes erecta) first reached Spain and from there it become popular as rose of Indies in Southern Europe. Major work on improvement and cultural practices on marigold have been done in USA, Switzerland, France and Germany. In India, marigold was introduced by Portuguese and it became popular and spread quickly because of its wide adaptability to varying climatic and soil conditions. Marigold is well distributed in different states and union territories of India (Singh, 2006).

SPECIES

Earlier, Rydberg (1915) reported that genus Tagetes comprises about 33 species and Bailey (1951 and 1976) mentioned over 30 species, however, Turner and Nesom (1993) reported that Tagetes is made up of nearly 55 species distributed in the American continent, most of them located in Mexico. The two species Tagetes erecta and Tagetes patula are commercially important. Details of these species are given below.

INTRODUCTION

Marigold is an important flower crop and constitute as one of the five most commonly cultivated and used flower in urban and rural India. They are extensively used for making garlands, beautification and other purposes i.e. pigment and oil extraction and thera eutic uses A art from these uses marigold is a widely grown plant in gardens and pots. It is highly suitable as a bedding plant, in a herbaceous border and is also ideal for newly planted shrubberies to provide colour and fill the space. French marigold is most ideal for rockery, edging, hanging baskets and window boxes. Flowers remain fresh for 4-5 days at room temperature and are used for religious offerings and social functions. Recently marigolds are grown commercially for extraction of carotene pigments mainly xanthophylls. The carotenoid extracted from petals are added to poultry feed for intensification of yellow colour of egg yolk. Lutein which is the major constituent of xanthophylls is used for colouring food stuffs. Purified extract of marigold petals containing lutein dipalmitate is marketed as an ophthalmologic agent under the name adaptinol. Dietary carotenoids can be used to treat cancer and photosensitivity diseases.

Marigold leaves contain a distinct odoriferous oil. Wild marigold (Tagetes minuta) oil has valuable and precious essential oil which is used in high grade perfumes and cosmetics. Ketones present in the oil are base material for synthesizing aroma chemicals. Marigold essential oil has bronchodilatory, tranquilizing, anit-inflammatory as well as insect repellent activities. The floral extract of marigold is used for treating eye diseases and ulcers.

Abstract: Among the annual flowers, marigold is commercially important loose flower crop in India. Marigold types with odourless foliage with orange to light lemon colour are available in world market. Being cross pollinating and seed propagated, progeny can segregate widely for traits due to heterozygosity. Improvements in marigold as cut flowers, larger flowers, more intense flower colour, an expanded flower colour range, more uniform plant habit, earlier flowering, more xanthophylls and extended bloom time are future breeding objectives. The use of intraand interspecific hybridization, heterosis, polyploidy and genetics of traits have been presented with a focus on creating novel transformations for this crop in future.

Introduction

Marigold is an important flower crop in India. They are extensively used for making garlands, beautification and other purposes i.e. oil extraction and therapeutic uses. Apart from these uses marigold is a widely grown plant in gardens and for pots. Marigolds are also grown commercially for extraction of carotene pigments mainly xanthophylls. The carotenoid extracted from petals are added to poultry feed for intensification of yellow colour of egg yolk. Lutein which is the major constituent of xanthophylls is used for colouring food stuffs.

Marigold plantation has been found beneficial to reduce the population of nematodes. Root-knot (Meloidogyne spp.) can be effectively managed in the field without pesticides.

Genetics

Marigold belongs to family Asteraceae. There are about 33 species of the genus Tagetes. Important species are T. erecta, T. patula, T. tenuifolia, T. lucida, T. lacera, T. lemmonii, T. minuta, T. pusilla and T. corymbosa. The chromosome number in diploid species i.e. T. erecta, T. tenuifolia, T. elongata, T. lemmonii, T. jaliscensis and T. lucida is 2n = 24, however, tetrapoid species i.e. T. patula, T. minuta, T. remotifolia, T. mendocina and T. biflora is 2n = 48.

Marigold (Tagetes spp.) is an important annual flower crop; native to Central and South America especially Mexico and belongs to family Asteraceae (Compositae). It is commonly known as “Gainda” in India and also called as “Friendship Flower” in United States of America. Loose flowers of marigold are in great demand throughout the year. It is commercially grown in tropical and subtropical regions of India owing to its ease in cultivation, hardy nature, wider adaptability and year round availability of single, semi-double and double flowers of vibrant colours. Marigold is grown over an area of about 66.09 thousand hectares with production of 603.18 thousand MT loose flowers in India. Major states involved in loose flower production of marigold are Karnataka, Madhya Pradesh, Gujarat, Andhra Pradesh, West Bengal, Maharashtra, Haryana and Chhattisgarh. In India, Assam is the only state, which produces marigold for both cut as well as loose flower purpose on an area of about 0.61 thousand hectare (Anonymous, 2016a).

Marigold is a cross-pollinated flower crop and it is pollinated by insects (Kaneko et al., 1990). It bears terminal and solitary inflorescence, known as head or capitulum, which possesses long and hollow peduncle with swollen apex and campanulate involucres (Setshogo, 2005). Capitulum consists of peripheral ray florets and central disc florets. Ray florets are pistillate, ligulate, sessile, epigynous and zygomorphic, whereas, disc florets are bisexual, tubular, sessile, epigynous and actinomorphic (Subrahmanyam, 1996 & Setshogo, 2005).

African marigold (Tagetes erecta L.) is a diploid species (2n = 2x = 24), while, French marigold (Tagetes patula L.) is a tetraploid species (2n = 4x = 48) with basic chromosome no. of x = 12 as revealed by breeding work of various researchers (Towner, 1961, Chen and Lin, 1981, Chen and Lin, 1982, Serrato-Cruz et al. 2000, Zhang et al., 2011). Tagetes erecta and Tagetes tenuifolia are diploid species, whereas, Tagetes patula, Tagetes minuta, Tagetes biflora and Tagetes remotiflora species are tetraploid in nature (De, 2017). On the basis of floral morphology; three different types of male sterile systems i.e. apetaloid, petaloid and gynomonoecious types was observed in African marigold (Prakash et al., 2016). Out of these, apetalous male sterility (femina type) is more reliable and preferred over petalous male sterility because later is more prone to break down (Goldsmith, 1968 & Janakiram, 2004). Apetaloid male sterility was controlled by single recessive gene (Prakash et al., 2016). A monogenic recessive male sterile apetalous mutant was detected from T. erecta population by Khoshoo (1979). Cyto-genetic male sterility is also found in marigold (De, 2017). Petaloid and gynomonoecious male sterile systems were controlled by cytoplasmic inheritance (Prakash et al., 2016). In marigold, cytoplasmic genetic male sterile lines were also developed for commercial production of F_l sterile hybrids (Pratibha et al., 2000).

ABSTRACT

The Mumbai region of Maharashtra has been witnessing very rapid urbanization development in terms of industrial, commercial and residential establishments. This has resulted in reclamation of tidal flats and estuarine regions. The ‘marine clay', characteristic of this environment has high swelling potential and consequently affects the performance of the structures. This will have a serious negative impact on the urbanization expansion. In this study, an attempt is made to map the spatial distribution of these expansive clays using the state of art Hyperion data. Reflectance spectra of representative samples collected from the field is used to map marine clays using Spectral Angle Mapper (SAM) technique. Laboratory analyses of these foundation soils (reflectance spectroscopy, mineralogy and Atterberg limits) indicate that they have significant amount of montmorrilonite and has good swelling potential (A_C>1.4). The derived expansive clay abundance map will be of use while planning appropriate remedial measure and future development.

Key Words : Swelling clay, Spectral characters, Geotechnical properties

1. I Kilo joule - 10 joule.

2. 1 Mega joule -10º joule.

3. 1 Kilowatt hour-3.6*10 joule.

4. The heat engine converts heat energy into mechanical energy.

5. The machine which converts one forms of energy to another form is called as engine or prime mover.

6. Fahrenheit scale is called as British thermal unit and denoted by B.Th.U.

7. Internal combustion engines using coal gas as fuel was made by Frenchman Etienne Lenoir (1860).

8. Silent gas engine was developed by Germans Nileolansotto & Eugen Langen.

9. Rudolph diesel made the first diesel engine in 1894.

10. TDC is also called as inner dead centre.

The resources on the land have started showing sign of faster depletion owing to the population explosion, over exploitation of mineral and water resources and also the improper exploitation and the related wasting of the natural resources. Hence a time has come to search for more and more newer natural resources amongst which the ocean resources gain supreme importance as this treasure is yet to be hunted due to the non - availability of suitable technology for the same.

Such ocean resources mainly include the mineralogical and biological resources. The ocean biological resources, their birth, growth, population and migration greatly depend as chlorophyll content, total dissolved oxygen, total suspended solids, salinity etc. These parameters which constitute the marine environment drastically change in a quick span of time owing to the dynamically changing marine forces.

Abstract

GIS in marine fisheries has taken off much later than its application in land. But the strides made in this field since 1990’s has been quite significant, as many serious mapping studies and modeling initiatives have been recorded. With the third dimension added to the 2D spatial data, the marine GIS have all the ingredients of making it a fertile domain for research for decades to come. Most of the studies and products pertaining to GIS in fishery management had been revolving around mapping of habitats, geo-referencing of catch and effort data and modeling linking oceanographic and biogeochemical factors with living resources. Satellite remotely sensed information like ocean colour can act as a surrogate to fish biomass. Further remotely sensed SST, wind and other parameters can be effectively modeled to predict fish abundance, their habitat and related aspects. This manuscript pours into the various products and outputs based on GIS in marine fisheries around the world and the Indian initiatives.

Key words: GIS, marine resources, oceanography, primary productivity and climate change

1.0 Introduction

Geographic Information Systems (GIS) have been one of the most prolific scientific enhancements in recent past, which by virtue of out of box expansion of traditional database concept have opened up an entirely new vista of knowledge and opportunities. It’s probably one such unique application where information collation and analysis happen hand in hand resulting in palpable mining of data in perspective. A formal introduction to GIS would define it as a collection of computer hardware, software, data and personnel designed to collect, store, update, manipulate, analyze and display geographically referenced information. By virtue of its conceptualizations, there are no single GIS; systems can be assembled in an infinite number of ways. This is one such field, which probably always seeks the frontiers of innovations in the fields of ICT and modeling.

ABSTRACT

The world ocean with a coastline of approximately 3,50,000 km is the largest ecosystem on earth. Marine organisms are the major, sustaining components of ecosystem processes and are responsible for biogeochemical reactions that drive our climate changes. Despite this, many marine organisms are poorly described and little is known of broad spatial and temporal scale trends in their abundance and distribution. With new molecular and analytical techniques we can advance our knowledge of marine biodiversity at the species level to understand how marine biodiversity supports ecosystem structure, dynamics and resilience. We can then interpret environmental, ecological and evolutionary processes controlling and structuring marine ecosystem biodiversity. In biotechnology applications marine microbes represent a potential source for numerous commercially important biomolecules and their bioremediation capabilities are also remarkable. They also play a crucial role in decomposition of organic matter and cycling of nutrients. Microbes also serve as food for some bottom-living organisms. Understanding and preserving biodiversity was one of the most important global challenges for the past 20 years and will continue to be an important scientific issue into the new millennium. Our knowledge of marine microbial diversity has, however, been severely limited by relying on microorganisms that have been cultured which are less than 1%.

Increasing pressures exerted on the environment by humans make protection of natural areas crucial for the preservation of biological diversity. Protected areas are one of the most effective tools available for conservation of biodiversity.

The IUCN defines a Protected Area as ‘an area of land and/or sea specially dedicated to the protection and maintenance of biological diversity, and of natural and associated cultured resources, and managed through legal or other effective means.

Since 1986, the IUCN commission on National Parks and Protected Areas (CNPPA) has been promoting the establishment and management of a global representative system of Marine Protected Areas (MPAs) which play a critical role in the conservation of biodiversity. MPAs are growing in importance globally as practical and potentially effective options for the management of fisheries, the protection of biodiversity and the generation of income from eco-tourism. The effective management of MPAs to ensure that they meet their declared objectives poses many challenges.

The IUCN has defined an Marine Protected Area (MPA) as ‘Any area of the intertidal or subtidal terrain, together with its overlying water and associated flora, fauna, historical and cultural features, which has been reserved by law or other effective means to protect part or all the enclosed environment (Kelleher,1999).

Introduction

The traditional approach to transferring genes from wild to cultivated species is based on interspecific hybridization followed by selection of hybrids that combine the trait with the cultivated genetic background. This breeding strategy is achieved by various backcross generations in which the selected hybrids at each generation are crossed back to the cultivated genotype with the aim of reducing the wild genome and its undesirable traits. The use of molecular markers has allowed this breeding approach to be greatly improved, since these markers directly reveal genetic variability through DNA analysis (Staub et al. 1996), and therefore their detection is not influenced by environmental effects. Since the development of numerous molecular markers for plant genometatic analysis, the possibility to select the genotype instead of the phenotype has been closely examined, leading to the concept of molecular marker-assisted selection (MAS; Paterson et al. 1991). The basic concept of MAS for crop improvement has been well discussed by Collard et al. (2005) which is most appropriate for this chapter. The most widely used markers suitable for MAS are RFLP, RAPD, AFLP, and SSR. Their common origin is point mutation or chromosome rearrangements that were accumulated during the evolution of the species without negatively influencing their survival and reproduction. The choice of the most suitable markers for MAS can differ and depends on the labor required for their detection, possibility of revealing single or multiple loci, dominant or co dominant nature and costs.

Marker Assisted Selection (MAS)

Marker Assisted Selection [MAS] is defining as the indirect selection for a desired plant phenotype based on the banding pattern of linked molecular (DNA) markers. It is based to infer the presence of a gene from the presence of a marker which is tightly linked to the gene of interest and high heritability of the gene of interest. Marker assisted selection (MAS) is also known by other names as marker aided selection and marker assisted breeding (MAB).

25.1 Introduction

The method of selection in which a gene/QTL (Quantitative Trait Locus) is selected on the basis of the genotype of the molecular marker (also called DNA marker) linked to this gene/QTL is known as marker-assisted selection (also called marker-aided selection). The idea of marker-assisted selection (MAS) was first advocated by Tanksley (1983). MAS can be used in combination with the conventional breeding methods or novel breeding schemes could be devised to take full advantage of the marker data. MAS has been widely used for introgression of the desired genes and QTLs using backcross programmes.

25.2 Marker-Assisted Backcrossing (MABC)

The backcross programme based on molecular marker is usually called marker-assisted backcrossing (MABC). MABC has three activities: (a) foreground selection, (b) background selection and (c) recombinant selection.

25.2.1 Foreground Selection

The use of molecular marker to select the gene/QTL being introgressed is known as foreground selection. The objective of foreground selection is to select the target gene, i.e., the gene being introgressed, without phenotypic evaluation for the trait governed by the target gene. Foreground selection for a gene/QTL will be highly desirable in a variety of situation, some of which are listed below:

ABSTRACT

In last twenty years, molecular biology has revolutionized conventional plant breeding techniques in many areas. The use of molecular markers in conventional breeding techniques has also improved the accuracy of selection and allowed breeders to produce varieties with combined traits that were difficult before the advent of DNA technology. Among vegetables, onion (Allium cepa L.) is the most important cash crop of India. Onion productivity in India is much lower (11.38 tones / ha.) compared to other countries (44.1 tones / ha. in Japan). Purple blotch disease (PBD) caused by Alternaria porri (Ellis) Ciferri decreases the yields by up to 50 percent. Genetically resistant onion varieties are the most economical, simple and eco-friendly mode of disease management. The advent of mo- lecular markers provided the opportunity to understand the genetic resistance more pre- cisely. In this paper we present our successful efforts in developing PCR based markers linked to purple blotch disease resistance, so that such markers can be easily integrated into PBD resistance breeding program in onion. Onion germplasm was screened for PBD resistance. Line PBR 287 was identified as a good source of resistance and the genetics of inheritance of resistance in this line was worked out. The protocols for isolating good quality DNA and PCR amplification conditions were standardized. 37 polymorphic mark- ers were identified between resistant and susceptible parents. Crosses were made between resistant and susceptible parents. Parents, F₂ and individual F₂ progenies were subjected for analysis of genetics of resistance and RAPD marker analysis. F₂ individuals segregated in 3:1 ratio for resistance. DNA from resistant and susceptible F₂ individuals was pooled separately for Bulked Segregant Analysis (BSA). BSA identified one RAPD marker of 900bp being linked to resistance. Further Chi-square test for detecting the deviation of segregation of this 900bp marker confirmed segregation with classical Mendalian ratio 3:1 for a monogenic trait. Identified marker is of great practical utility to the plant breeders to screen large number of populations for purple blotch disease resistance at the early stages of the crop even in absence of the pathogen.