eChapters

Introduction

The digital farming solutions by various advanced tools and technologies became a prime important to see the future smart agriculture in India with enhanced productivity and farmer’s lifestyle upliftment. The agriculture industry facing challenges from farm to market by climate interfacing and energy conservations practices during farming operations. The use of innovative industry 5.0 is targeting a farming society to achieve high productivity by hardware devices like Robots, Drones, Automated Guided Vehicles (AGVs) working on the basis of Artificial Intelligence (AI), Machine Learning (ML), Deep Learning (DL), Big-Data, IoT and Cloud-based software tools and interactive software programs.

The use of tractors, combine harvesters, power tillers etc. like advanced machineries are practiced in present farming practices by youth farmers; however the digital tools with an automation of existing advanced farm machinery became an attraction to see human comfort. The custom operative design and development of digital tools are essentially becomes a need to innovate and exercise in finding best technologies for Indian farming society. The agricultural society spent several years and now also synthesizing an operational description for digital farming. There are several ways of opting solutions by using new digital logistic tools with software and hardware engagements. As we know agricultural production is influenced by climate, weather, soil properties, plant genetic profile, plant peripheral environment, plant protection, plant nourishing, harvesting and post harvesting like operational tasks. All such a challenging task can be handled by advanced digital tools and technologies.

Introduction

More than 380 million urban population living in town and cities generates 62 million tones of municipal waste every year out of which 75% is recyclable but only 30% is recycled and reused whereas less than 15% of municipal solid waste is treated. Generally municipal authorities of the country dump the waste outside the city to keep the urban area clean. According to World Bank report, by the year 2025, waste generation will reach 377,000 tonnes per day in India (CPCB 2020). The waste generated in industrial and urban area of the country is tangible and concerning. This is the high time to address this issue with a great sensitivity and planning. Ragpickers works as the informal waste collection and recycling sector which plays an important role in waste management and leads to lesser waste to the landfills (Gupta et al., 1998).

Waste management is the process of treatment and disposal of solid, organic and hazardous waste. It can be categorized based on the source sector i) urban waste; ii) electrical waste iii) medical waste; iv) waste produced by disasters; v) agricultural waste vi) industrial waste etc. The type of waste has variable potential harms for human health, social implications, policies and the environment (FICCI, 2011). Agriculture itself is a big source of waste generated at different paths of food supply chain that occurs as follows:

Introduction

The post-harvest and marketing system comprised of a series of interconnected activities which starts after the harvesting process and continues until the distribution of food products to the consumers. An effective post-harvest system makes sure that the food product reaches the customer in a short time without sacrificing volume, quality, and safety of the food product (Gardas et al., 2018). Increasing population growth in the last few centuries and the requirements from industrialization forces the need for sustainable food production and better food processing technologies. There is a requirement of balance between sustainable food production practices, a healthy environment, and a healthy population due to the rapid changes in the climate and population. According to the Food and Agricultural Organization (FAO), there will be a requirement of a 70% increase in food production in 2050 due to the growing population across the world (FAO, 2017).

The challenges such as improper land use, decrease in biodiversity, aquatic eutrophication by over-fertilization, global warming, water shortages due to improper irrigation etc. imposed awareness among the stakeholders to bring more efficient approaches in agricultural and food production practices. This brings the idea of terms such as recyclable and eco-friendly packaging, buy local, whole foods, free-from, and fair-trade (Boye and Arcand, 2013). Recent trends in climate and changes in the environment may pose challenges to achieve the required food production in the future. In addition to the steps taken for increasing food production, the steps to reduce food loss are important. In 2011, it was calculated that 11.3 billion tonnes of food was wasted in a year, which is almost one- third of the total food production (FAO, 2011).

Introduction

Animal husbandry has been an integral component of Indian agriculture since time immemorial. It is still continuing as one of the major avocations in India contributing nearly 4.9% to the national GDP and 28.4% of the agricultural GDP. Livestock population in the country as per Livestock Census 2019 is 535.78 million which is an increase of 4.6% over Livestock Census 2012 (BAHS, 2019; DAHD, 2019). Cattle, buffaloes, sheep and goats are the major livestock in the country. Today, India is the global leader in milk and milk products with a production of 187.7 million tonnes (MT) and the availability of 394 ml milk per capita per day. The per capita availability of milk has increased substantially during last four decades. The anticipated milk demand is expected to be 266.5 MT by 2030 based on per capita consumption of 468 ml/day (Table 1). It has been made possible with the active involvement of farming community and by creating awareness about high yielding breeds of livestock, improved rearing methods and milking, management of pests and diseases and most importantly, the feeding of the animal. The crop residue constitutes a major chunk of fodder to the livestock in many parts of the country. However due to their low nutritive values and poor palatability, it is not helping to realize the full milking potential of animals. A huge opportunity exists in livestock sector as there is a growing demand for animal products particularly milk and meat (NDDB, 2019). In addition, for promoting organic farming particularly the need of farm yard manure has necessitated the farming community to keep livestock as a complementary component of his farming. It provides draught power and organic manure to crop sector and hides, skin, bones, blood and fibers to the industrial sector. Therefore, the importance of livestock goes beyond its food production function, and has become even more significant (Birthal et al., 2002).

Introduction

The Indian fisheries sector is set in a unique and diverse set of resources ranging from the pristine waters of the Himalayas to the sprawling Indian Ocean. The fisheries biodiversity of the country encompasses a wide spectrum of physical and biological components that support the livelihoods of millions of people. Fisheries resources are set in different ecosystems. With growing population and the increasing demand for fish protein, the need for sustainable development of aquatic resources is now felt much more than ever before.

Fisheries and aquaculture remain important sources of food, nutrition, income and livelihoods for hundreds of millions of people around the world. Global capture and culture fish production during 2019 were 97 and 82 million tonnes, respectively with India being second largest producer of fish in the world after China. From 0.75 million tonnes in 1950-51, total fish production of India touched 12.59 million tonnes in 2018-19 with a contribution of 8.90 million tonnes (70.60%) from inland sector. The sunrise fisheries sector contributes about 0.96 % to National Gross Value Added (GVA) and 5.37% to agricultural GVA (2018-19) while engaging over 14.5 million people (2003 census) at the primary level and almost twice the number along the value chain. During 2018-19 the export of marine products that includes production from aquaculture reached 13,77,244 tonnes valued at INR 46589 crore (US $ 6.93 billion). The inland sector grew at a compounded annual growth rate (CAGR) of nearly 6.5% between 1979 and 2018, while overall fish production witnessed 4.5% growth, which is double the growth rate of food grains production in India.

Introduction

India is predominantly an agricultural economy and about half of the workforce is engaged in agriculture sector. Around 48 per cent of households in the country are agricultural household and they derive the monthly income from crop cultivation. India is leading in production of many agricultural crops but finding a market for marketed surplus and getting fair prices have always been a major challenge. Long time ago, traditional market system was existed in India. Marketing implied greater magnitude as yields and production started rising after farmers embraced with advanced technologies such as high-yielding varieties, fertilizers, chemical inputs, block chain development, farmers business network, market linkages, diversification towards high value crops, integrated farming system approach, precision farming including digital agriculture, drones, GIS technology, remote sensing, soil health management, crop and livestock insurance, mobile apps, web portal, machine learning and many mores. These catered the need of efficient regulated marketing system to support the farmers and keep away from them getting manipulated in the market.

India has gained self-sufficiency in the agriculture production. There is need to translate this production into better remuneration for the farming community by focusing into agricultural marketing system. The agricultural marketing system is characterized by various short comings like heavy sale of commodities at primary level immediately after the harvesting period, absence of on-farm grading of produce, poor packaging, insufficient marketing infrastructure, long marketing channels, existence of various malpractices in the marketing of agri-produce, nontransparent price discovery mechanism, lack of market information system, low marketable surplus especially of small and marginal farmers, etc.

Rising population and increasing demand for food are prominent factors driving the digital farming market. Moreover, the growing demand to efficiently deal with pests, weeds, and other diseases is encouraging farmers to digitize their farm. In developing regions, cheaper internet data and strong penetration of smart devices (especially smartphones) are driving the digital farming market. Active participation of governments in digitizing farms through investment is also driving the digital farm market.

Introduction

The food production in India has been on a rising trajectory and the country is among the top producers of several crops. It is the highest producer of milk and second highest producer of fruits and vegetables. Total food grain production in the country is estimated at record 291.95 million tonnes (MT; Second Advance Estimates for 2019-20) which is 6.74 MT higher over 2018-19. While enhanced field productivity is an essential component of agricultural sector, improved postharvest handling, marketing and processing is equally essential to reduce the waste, particularly in a country where such losses are substantial and add stress on limited farming resources, appropriate agrilogistic is needed. Post-production management and marketing infrastructure is therefore, not only vital but a priority. Such infrastructure and the services they provide are essential not only for the efficient performance of various marketing functions, but also for effective expansion of the size of markets which allows for growth.An efficient post-harvest logistics network not only ensures that produce will reach destination markets in quality and quantity, but should also trigger a steady reverse flow of market information to the source.

Introduction

Agricultural Extension is as ancient as agriculture itself. When it moves in agricultural trends, the importance on extension has also been transformed. Firstly, agricultural extension was the component of the research-extension-farmer approach. In the Training and Visit (T&V) period, communication of innovation was the crucial concept and the role of extension was to successfully disseminate the technological interventions. However, after the green revolution, the gap between resource rich and resource poor farmers widened with information and extension reaching only a few of them. With the conceptualization of new models, like Agricultural Knowledge and Information Systems (AKIS), the focus of extension has changed to formation of groups and collective farming. In time, the private sector has also entered into the agricultural arena, introducing the concept of pluralism in extension. Emphasis on mass media has also increased. More recently, Agricultural Innovation Systems (AIS) is introduced within the innovation systems framework. It focuses on the actors, their interactions, the institutions, and the policy environment to support or hinder it. Communication for innovation is the key AIS concept tried to identify the actors in systems, their core activities and bring them into an innovation platform for effective service and successful innovations (Saravanan, 2017).

Historical Perspective of Agricultural Education Policy in India

Pre-Independence Era

During the reign of the British Crown, vocational education and training suffered a great setback and there was a lack of emphasis on agricultural education, even though the Indian economy, to a great extent, depended on agriculture. In 1901, only five institutions were imparting theoretical and practical instruction in agriculture, but that education was not used in extension or fieldwork—it served only as an opening for government white-collar jobs (Crane, 1965).

Post-Independence Era

Prior to the establishment of agricultural universities, university-level education in agriculture, animal science, horticulture, agricultural engineering, and allied subjects were largely conducted by colleges affiliated with general universities that had no special commitment to the farming community. The University Education Commission of 1947, among other things, examined the state of agricultural education in India. The report of this Commission emphasized the need for having what was termed “Rural Universities.” In 1954, the First Joint Indo-American Team on Agricultural Research and Education fully endorsed the recommendations of the University Education Commission and strongly recommended the immediate establishment of agricultural universities in a few selected states. The U.S. agricultural advisors who began to arrive in India in the early 1950s were products of the land-grant university system, and many of them naturally believed India could benefit by creating a similar system (Goldsmith, 1988). The Government of India accepted this recommendation and the first agricultural university in Uttar Pradesh now named as Govind Ballab Pant of Agriculture & Technology was established in 1960, patterned on the land-grant university model of the United States. This was a significant turning point in the history of agricultural education in India. The Second Joint Indo-American Commission (1959) reiterated the recommendations of the First and recommended that at least one agricultural university be established in each state. Agricultural universities have now been established in 21 of the 28 states and the concept of the agricultural university now forms an essential part of the national policy on education.

Introduction

India is considered as one of the emerging superpowers of the world. This potential is attributed to several indicators, the primary one being its demographic trends and a rapidly expanding economy. In 2015, India became the world’s fastest growing economy with a 7.5% estimated GDP rate. But in the year 2020, the pandemic lockdown situation created adverse impact on the global economy. At this juncture, there is an urgent need to focus on native and indigenous resource mapping and judicious utilization of it to be a self-reliant. The COVID-19 pandemic has necessitated a new set of reforms to strengthen the economy. The first mention of this came in the form of the ‘Atmanirbhar Bharat Abhiyan’ or ‘Self-Reliant India Mission’ by the Government of India during the announcement of the coronavirus pandemic related economic package on 12^th May 2020. Almost 70% of India’s population depends directly and indirectly on agriculture for their survival. This means the country cannot have a development model that ignores agriculture.

It’s time to take a look at India’s agriculture economy. Over the last 10 years, (2008-19) India has been a net exporter of many farm produce. In fact, in 2018, the government has come up with a new agricultural export policy aimed at doubling farm income by 2022. However, in 2019-20, agri-exports were just $36 billion and the agri-trade surplus was $11.2 billion. The net agri-trade surplus has been falling over a period of time, making it difficult to achieve doubling of farmers’ income and doubling of agriculture exports by 2022as well.

India is not yet self-reliant in various essential agriculture commodities and depends heavily on imports. India imports around 15 million tonnes of edible oil every year — more than 9 million tonnes of palm oil and about 2.5 million tonnes each of soybean oil and sunflower oil. It buys palm oil from Indonesia and Malaysia, and soy and sunflower oil from Argentina, Brazil, Ukraine and Russia. There is a need to create self-reliance in theses commodities enhancing productivity of Indian farms to create competitive advantage for domestic producers. India can step up cultivation of sunflower, groundnuts, mustard and oil palms. Presently, India has about two million hectares of land that is suitable for oil palm cultivation.

Introduction

India is transforming from the phase of revolution to commercial mode of agriculture, a step ahead of self-sufficiency. It further evolves to reach a goal of self-reliant on agriculture and allied sector. The present phase demands several technological innovations, their application and implementation in the market. Meanwhile this phase needs due attention for handling the issues on conservation of natural resources, especially germplasm resource, biodiversity conservation, socio-cultural-economic values of our country, etc. The whole process requires good investment on research and development, various economic measures and implementation. It is only possible with vibrant governance and policy implementation of a Government. Already Government of India has taken several measures to transform agriculture. Recent analysis showed that Indian agriculture is the silent ever green revolution. Food production is kept pace with the growing population since independence. Further, it needs several innovations on technologies coupled with policy support to fill the demand. India is placed 49^th position in the world on innovations. Analysis suggested the huge potential for innovation due to its huge human capital, which perhaps need for the country like India to meet the food demand of 333 million tonnes by the year 2050. These innovations must be focussed for growth to efficiency, employment generation, food security to nutrition and health, shortage management to surplus management, input intensive to knowledge intensive agriculture, climate change and sustainability, production and producers, policy interventions, regulations and reforms. The Government emphasize usage of e-trading platforms to sell the farmers’ produce of their choice rather using mandis. This will enable contract farming and inter-state trade. However, major innovations and investment must be implemented on transportation, logistics, and digital access and payments to enable widespread prosperity. Thus, focus must be on the complete agricultural value chain. In fact, focus needed on value addition, rural infrastructure, logistics, warehousing, and quality certification of agriculture produce. India has greatest challenges to face in the future for food and nutritional security, adaptation and mitigation to climate change and sustainable use of essential and scared resources like water, land and energy.

The basic principles of biological control of pests and diseases remain the same, whether applied to insect pests, nematodes or plant pathogens. In the recent years, there is a paradigm shift towards studying antibiosis and its application in biological suppression of pathogens, yet the classical bio-control will remain as relevant as ever. The following discussion is based on recapitulating the basic principles of classical biological control as applicable to plant parasitic nematodes.

Biological control agents (BCAs) have been exploited for pest control in different ways. One way is to manipulate the naturally occurring BCAs by augmenting their populations to pest suppressive levels. In this approach no BCAs are introduced, but various means such as agronomic practices, introduction of organic matter etc. are adopted to favour the build-up of BCAs. The second way is the introduction of BCAs, either as inoculative or inundative release. In the former case, the BCAs are introduced in small quantities and allowed to build-up gradually in due course of time. However, in the latter approach, BCAs are introduced in large quantities so that quick pest suppression can be achieved. Each method has its own merits and demerits (Table 1).

Introduction

The soil around plant roots that forms the rhizosphere is a dynamic, complex zone. All plant parasitic nematodes are obligate parasities and must enter this zone to reach their host and cause damage (Kerry, 2000). Nematodes in soil are subject to infections by bacteria and fungi. This creates the possibility of using soil microorganisms to control plant-parasitic nematodes. Bacteria are numerically the most abundant organisms in soil, and some of them, for example members of the genera Pasteuria, Pseudomonas and Bacillus have shown great potential for the biological control of nematodes. Nematophagous bacteria are distributed broadly, possess diverse modes of action, and have broad host ranges. A variety of nematophagous bacterial groups have been isolated from soil, host-plant tissues, and nematodes and their eggs and cysts (Siddiqui and Mahmood, 1999; Kerry, 2000; Meyer, 2003).

They affect nematodes by a variety of modes: for example parasitizing; producing toxins, antibiotics, or enzymes; interfering with nematode–plant-host recognition; competing for nutrients; inducing systemic resistance of plants; and promoting plant health (Siddiqui and Mahmood, 1999). They form a network with complex interactions among bacteria, nematodes, plants and the environment to control populations of plant-parasitic nematodes in natural conditions (Kerry, 2000).

Introduction

Plant parasitic nematodes are important pests, causing major damage to the world’s food and fibre crops. The management of nematodes is more difficult than that of other pests because nematodes mostly inhabit the soil and usually attack the underground parts of the plants. Nematodes in soil are subject to infections by bacteria and fungi. This creates the possibility of using soil microorganisms to control plant-parasitic nematodes. Biocontrol of nematodes was first studied by Duddington (1951). The development of biological control agents is also considered an effective alternative for nematode control on vegetables (Van Gundy, 1985; Kerry, 1987). There are more than 200 species of nematophagous fungi described. Based on the infection mechanism, they are commonly subdivided into three main groups: the nematode-trapping fungi that captures free living nematodes using specialized morphological structures (i.e., traps), the endoparasitic fungi that infects nematodes using adhesive spores, and the egg- and cyst-parasitic fungi that infect these stages with their hyphal tips (Barron, 1977). The nematophagous fungi that have received most attention for biological control of plant-parasitic nematodes include various species of the nematode-trapping fungi Arthrobotrys spp. (Stirling and Smith, 1998), and the egg-parasitic fungi Pochonia chlamydosporium (Kerry, 2001) and Paecilomyces lilacinus (Gaspard et al., 1990).

Introduction

The need of the agricultural community was the force of motivation for the development of broad-spectrum pesticides towards the management of pests and diseases of cultivated crops. Advances in pesticide were measured exclusively by the assessment of its efficacy in management alone. But today food production has ceased to be a major concern, due to stable population levels and success of innovative agricultural technologies. At this juncture, the agrochemical industry that primarily recognized the farmers as their customers has failed to acknowledge the public as an important client. Perception of the public reflects that, the negative aspects of pesticides seem to outnumber their benefits. The increased reflection on environmental concern over pesticide use has been instrumental in a large upsurge of biological disease control. Development of fungicide resistance among the pathogens, ground water and foodstuff pollution and the development of oncogenic risks has further encouraged for the exploitation of antagonistic microflora in disease management

Introduction

Advances in pesticide were measured exclusively by the assessment of its efficacy in management alone. But today food production has ceased to be a major concern, due to stable increase in population levels and success of innovative agricultural technologies. At this juncture, the agrochemical industry that primarily recognized the farmers as their customers has failed to acknowledge the public as an important client. Perception of the public reflects that, the negative aspects of pesticides seem to outnumber their benefits. The increased reflection on environmental concern over pesticide use has been instrumental in a large upsurge of biological disease control. Development of fungicide resistance among the pathogens, ground water and foodstuff pollution and the development of oncogenic risks has further encouraged for the exploitation of antagonistic microflora in disease management.

Biological control of plant pathogens can be broadly defined as the reduction of inoculum density or disease producing activities of a pathogen or parasite in its active or dormant state by one or more organisms, accomplished naturally or through manipulation of environment, host or antagonist or by mass introduction of one or more antagonists. The decades of laboratory experiments have led to the explosion in the field of bio-control and introduced more than 50 commercial formulations comprising of fungi and bacteria. To have a successful management of plant diseases and to have a pollution free environment, the biocontrol agents has to be ensured for its availability for commercial use by the end users. To harness the potential of biopesticides by the stakeholders and end users the product has to be registered under Central Insecticide Board authorities concerned of the respective countries.

Introduction

A sustainable approach to managing pests by combining biological, cultural, physical and chemical tools in a way that minimizes economic, health and environmental risks. The objective for IPM practitioners is to minimize risks to human health and the environment from the pest management actions implemented. IPM is a holistic approach that seeks to manage pests by using methods that are effective, economically sound, and ecologically compatible. IPM practitioners base decisions on information that is collected systematically as they integrate economic, environmental, and social goals. IPM promotes the use and integration of biological control agent as one of the strategies for pest and disease management. Because, biological control of plant pathogen is a potential alternative to the use of environment harming chemical pesticides in agriculture.

To study the characteristics of microorganisms, it is essential to culture and growth them. Growth is the increase in cell number which can be obtained by providing proper physical and chemical conditions. Medium is a substance that provides nutrients for growth and multiplication of microorganisms. Any medium for the cultivation of bacteria and fungi must provide certain basic nutritional requirements which include a carbon, nitrogen, phosphate and various mineral nutrients. The following is the medium composition for the isolation and culturing of different biocontrol agents

The isolation of microbes is commonly done using serial dilution technique.

1. Trichoderma

Trichoderma is one of the important biocontrol agents. The colonies first produce mycelial growth and then turn yellow and finally produce a dark green color mat.

The discovery and use of synthetic chemicals has contributed greatly to the increase of food production industry by controlling pest and diseases. However, the use of synthetic chemicals has raised number of ecological problems apart from increasing the cost of cultivation. In recent years, scientists have directed their attention in exploring the potential of beneficial microbes for plant protection measures. Biocontrol agents exhibit antagonistic activity against plant pathogens and activate resistance mechanisms in the host despite improving plant growth. In agricultural soils the nutritional condition is depleted hence beneficial microbes decrease and increase the pathogenic microbes. To ensure the sustained availability of biocontrol agents mass production technique and formulation development protocols has to be standardized which also increases the shelf life of the formulation. Though these agents have a very good potential in the management of Pest and Diseases it can not be used as cell suspension under field conditions. The cell suspensions of these biocontrol agents should be immobilized in certain carriers and should be prepared as formulations. The organic carriers used for formulation development include peat, turf, talc, lignite, kaolinite, pyrophylite, zeolite, alginate, pressmud, sawdust, vermiculate, etc.

Bacteria are by far the common type of soil microorganisms, possibly because they can grow rapidly and have the ability to utilize a wide range of substances as either carbon or nitrogen sources. The bacteria that provide some benefit to plants are found near, on (or) even within the roots of plants (Kloepper et al., 1980) to protect the plants from pathogen attack called as antagonistic organism. Among the many potential bacterial antagonists associated with the plant roots the fluorescent pseudomonads have received prominent attention due to their abundance in plant rhizosphere and their ability to colonize roots of a wide range of crop plants. Pseudomonas fluorescens is a Gram negative, non spore forming, rod shaped bacteria producing pigments which are greenish, fluorescent and water soluble. It is found to be effective against the following fungal pathogens viz., Rhizoctonia solani, Macrophomina phaseolina, Fusarium udum, Pyricularia grisea, Fusarium oxysporum fsp. cubense. It is capable of surviving in the spermosphere, rhizosphere and phyllosphere of plants. The mechanisms of biological action exhibited inovles antibiosis, competition, lysis and induced resistance. Pseudomonas have an exceptional capacity to produce a wide variety of metabolites, including antibiotics, that are toxic to plant pathogens.

1. Raw Materials

Culture of Bacillus subtilis

Talc powder - 300 mesh

Shelf life for Pseudomonas fluorescens (TNAU Pf-1)

Aim: To find out the shelf life of P. fluorescens (TNAU Pf-1) using different carrier materials

Product: The talc based P. fluorescens (TNAU Pf-1) product is used for the study on shelf life.

Selective medium (King’B medium) for Pseudomonads

Deoxyribonucleic acid (DNA) carries the genetic instruction for the biological development and functioning of all cellular forms of life. It is the blue print which contains the instructions to construct other cell components. Molecular biology experiments often require isolation of DNA for a variety of purposes like construction of DNA libraries, gene cloning, PCR and other marker technology, etc. The extraction of DNA from actively growing tissue is relatively easier yielding higher quantities of DNA.

Principle

The cell wall is ruptured and the proteins associated with DNA are removed by detergent like SDS or by interaction with phenol. Lipids are extracted with alcohol and DNA is precipitated by isopropanol.

Materials Required

Maize seed samples, Liquid nitrogen, CTAB (Cetyl trimethyl ammonium bromide), Chloroform, isoamyl alcohol, Sodium chloride, Glacial acetic acid, Isopropyl alcohol and 70% ice cold ethanol, TE buffer (5X) (24.2 g of Tris base, 5.71 ml of galcial acetic acid, 10 ml of 0.5M EDTA (pH 8.0) , dissolved in 1000 ml of distilled water). Prepare 1X TE as per the requirement.

Many Bacillus species are capable of producing a wide variety of secondary metabolites that are diverse in structure and function. The production of metabolites with antimicrobial activity is one determinant of their ability to control plant diseases. The activity is determined using PCR. PCR consists of an exponential amplification of a DNA fragment, and its principle is based on the mechanism of DNA replication in vivo. dsDNA is denatured to ssDNA, duplicated, and this process is repeated along the reaction according to the following formula

Agarose gel electrophoresis is used for the fractionation and characterization of nucleic acids. It is a simple and highly effective method for separating, identifying and purifying DNA fragments of various sizes. DNA can be checked for size, intactness, homogeneity and purity by this technique. Agarose is a purified form of agar and forms a gel by hydrogen bonding of the agarose monomers. Increasing the Agarose concentration of a gel reduces the migration speed and enables separation of smaller DNA molecules. Shorter molecules move faster and migrate further than longer ones.

Aim: To study the size of the DNA extracted from the fungal culture.

Principle: Ethidium Bromide added to the gel interacts with the bases of DNA and fluorescence orange when irradiated with UV light.

Introduction

Studies on biocontrol agents have revealed the presence of a diverse range of antifungal metabolites derived from amino acid, polyketones and isoprenoid biosynthetic pathways, each of which has distinctive regulatory points and is subjected to different stimuli within organisms. In this experiment, the extraction of secondary metabolites from the culture of T. viride and Chaetomium globosum will be demonstrated.

Entomopathogenic nematode (EPN) viz., Heterorhabditis, Neosteinernema and Steinernema have been recovered from many regions of the world. They selectively infect many insects and a few other arthropods but do not adversely affect mammals or plants. They are actually vectors of pathogenic bacteria, cause relatively rapid death of the host (24 – 48 h) and show considerable potential in biological control of insect pests in the integrated pest management systems.

EPNs are similar to insect parasitoids in that the immature form develops at the expense of one host individual; they are ecologically similar to both parasitoids and arthropod predators because the host individually killed. However, these nematodes differ from predators and parasitoids in two ways: (1) the nematode is mutualistically associated with a bacterial pathogen which actually kills the host, and (2) the pathogenicity of the bacterium has a great influence on the efficacy of the system (Ehler, 1990). The purpose of this chapter is to introduce EPN as biocontrol agents in this refresher course and to provide an overview of some current issues in biological control of insects with particular emphasis on their relevance to the use of EPNs.

Biological control is defined as the action of natural enemies which maintains a host population at levels lower than would occur in the absence of the enemies. Natural enemies will include parasitoids, predators, nematodes, microbial pathogens and their gene products. Entomologists generally divide biological control into two categories: (1) natural biological control which is effected by native or coevolved natural enemies in the native home (origin) of a given insect and (2) applied biological control which due to human intervention. Applied biological control is further divided into classical biological control (introduction of exotic natural enemies) and augmentative biological control (enhancement of natural enemies already in place).

Introduction

Corcyra cephalonica commonly called as rice meal moth or rice moth is a pest of stored foods, viz., cereals, cereal products, oilseeds, pulses, dried fruits, nuts and spices. Many of the natural enemies mass-bred in the laboratory for use in field against crop pests are dependent on either egg or larval stages of Corcyra due to the simple reason that it is easier and cheaper to produce natural enemies on different stages of Corcyra than on their original hosts.

Morphology and Biology of Corcyra

The eggs are oval and measure 0.5 x 0.3 mm. The white surface is sculptured and has a short nipple-like structure at one end. The larvae are generally creamish – white except for the head capsule and the prothoracic tergite, which are brown. There are well-developed prolegs on abdominal segments 3-6 and 10. A fully matured larva measures 15 mm. The last-instar larva spins a closely woven, very tough, double-layered cocoon in which it develops into a dark-brown pupa. The anterior portion of the cocoon has a line of weakness through which the adult emerges. The adults are small. The hind-wings are pale-buff, and the fore-wings are mid-brown or greyish-brown with thin vague lines of darker brown colour along the wing veins. The males are smaller than the females.

Sexual activity usually begins shortly after adult emergence. There is a preoviposition period of about 2 days. Egg-laying mainly occurs during the night. The greatest numbers are laid on the second and third days after emergence, although oviposition may continue throughout life. Eggs take about 2-3 days to hatch. Optimum conditions for larval development of C. cephalonica are 30 – 32.5 °C and 70 per cent RH, at which, the period from egg hatch to adult emergence is only 26-27 days. There is considerable variation in the number of larval instars; however, males generally have 7 and females have 8. The last-instar larvae pupate within the food. The adults emerge through the anterior end of the cocoon, where there is a line of weakness. The sex ratio is 1:1. The adult moth is nocturnal and is most active at nightfall.

Although the Rhabditids have been studied from last one century, the field level use of Entomopathogenic nematodes the members of Rhabditids for the management of insect pest was followed only after Dutky who practically used EPNs in 1937 on Apple Codling moth.

The taxonomy of Entomopathogenic nematodes of the order Rhabditidae is discussed in this lecture.

Basically we need to mount and prepare the slide for observation of various stages of EPN as detailed below in Table.1

I. In vivo culturing technique

In vivo culturing means culturing of entomopathogenic nematodes (EPN) in the host. Generally rice meal moth, Corcyra cephalonica and greater wax moth, Galleria mellonella are used as insect hosts for rearing EPNs.Insect hosts such as Spodoptera, Helicoverpa and Bombyx mori are also used for culturing Steinernema and Heterorhabditis but these insects need plant host especially leaves for culturing. Corcyra can be grown in broken cumbu grains and Galleria in honey comb or artificial diet.

Introduction

Entomopathogenic nematodes (EPNs) viz., Heterorhabditis, Neosteinernema and Steinernema have been recovered from many regions of the world and have been used commercially as biocontrol agents of insect pests. They are associated with mutualistic bacteria in the genus Xenorhabdus for Steinernematidae and Photorhabdus for Heterorhabditidae. The infective juvenile nematodes enter their host insect through natural openings (mouth, anus or spiracles), they release bacteria into the insect and cause rapid death of insect host (24-48 h). EPNs were used against many insect pests viz., rice leaf folder, Cnaphalocrocis medinalis (Srinivas and Prasad, 1991); tobacco cutworm, Spodoptera litura (Rajkumar et al., 2003); brinjal fruit borer, Leucinodes orbonalis (Hussaini et al., 2002) diamond back moth, Plutella xylostella (Singh and Shinde, 2002); Diaprepes abbreviates (Jenkins et al., 2007); Bemesia tabaci (QiuBaoli et al., 2008) and sugar beet beetle (Saleh et al. 2009).

Introduction

Agricultural production in India is constrained by number of biotic and abiotic factors. India is a tropical country and has a climate which is conducive for insect pests which are, one of the major constraints in agricultural production, processing and storage. Annual crop losses due to insect pests and diseases in India are estimated to be 18 percent of the agricultural output. Insect pests are widespread in all the agroclimatic zones and large quantities of insecticides are used to control these pests. Indiscriminate use of insecticides, lack of adequate knowledge on proper application techniques and dosages, and frequent use of broad spectrum insecticides are common in India. There is a need to identify suitable alternative methods for the management of insect pests and among them, biological control using viruses, bacteria, fungi and entomopathogenic nematodes is gaining greater attention world over.

Nematode families of Steinernematidae and Heterorhabditidae attracted most attention as they contain the entomopathogenic nematodes (EPN). Entomopathogenic nematodes are obligate parasites of insects that kill their hosts with the aid of bacteria (Xenorhabdus and Photorhabdus) carried in the nematode’s alimentary canal. The lethal obligate insect parasites are ubiquitously distributed without pathogenicity to mammals and beneficial non-target insects, and exempted from registration requirements. Currently there are about 98 valid EPN species belonging to Steinernema (79) and Heterorhabditis (19). Soil surveys conducted in divergent areas of Indian subcontinent have demonstrated high diversity of EPN across seasons, habitats, and geographic regions. There are several target pests in India that can be controlled with entomopathogenic nematodes and several workers have used EPN against cutworms, stem borer, white grubs, etc. under laboratory and field conditions and are compatible with existing IPM programmes.

Entomopathogenic nematodes (EPN) in the family Steinernematidae and Heterorhabditidae have enormous potential to control insect pests of economic importance. Since, chemical pesticides pose environmental hazards, these nematodes have received considerable attention as potential bio-insecticides because of their wide host spectrum, active host seeking, killing the host within 48 h, easy mass production, storage and application and are environmentally safe. One of the important criteria for a successful biocontrol agent is formulation of nematodes into a stable product, which has played a significant role in commercialization of these biological control agents. Apart from formulation field application and desired control of insect pests decides the success of EPN.

Formulation of Entomopathogenic Nematodes

EPNs have been known since 1929 but they became commercially available only during 1990s.The first attempts at formulating EPN were initiated in 1979 but the shelf life was very limited (one month). Infective juveniles carried on moist substrates such as sponge, vermiculite and peat require continuous refrigeration to maintain their viability. While formulating nematodes, two things are necessary, a long shelf-life and easy application method. The simplest method is impregnating a moist substrate (such as sponge, peat, vermiculite, etc.) with nematodes. The sponge needs to be squeezed in water before application, to release the nematodes, whereas nematodes from other carriers could be applied directly to the soil. However, these formulations are labour-intensive, require continuous refrigeration and lack economy of scale and therefore can be used on small scale only.

Although the infective juveniles of entomopathogenic nematodes can be stored for several months in water in refrigerated bubbled tank, high cost and difficulties of maintaining quality preclude the deployment of this method. Therefore, nematodes are usually formulated into solid or semi-liquid substrates soon after they are produced.

Horticulture is the science and art of production, improvement and utilization of fruits vegetable, ornamental, aromatical medicinal, spices and plantation crops. The word horticulture derived from latin words ‘Hortus’ means garden and culture means cultivation.

The horticulture science can subdivided into different following branches on the basis of product utilization and crop involved.

Pomology: This word is derived from latin word pomus ‘Fruit’ and Greek term logy ‘Science’. The branch deals with the cultivation of fruits called pomology.

Olericulture: It is the branch of horticulture which deals with cultivation of vegetable crops product of which is utilized for culinary purpose after cooking. Olericulture word is derived from the latin word oleris means pot herbs and the english word culture means the culture of succulent vegetables.

Plantation crops: Cultivation and processing of tropical crops grown on a large scale centered around a factory constitute the plantation crops e.g., tea, coffee, black pepper.

Spices crops: These crops are grown and used for providing fragrance and flavouring to various food stuffs e.g., cinnamon, clove, pepper, coriander etc.

Medicinal plants: These plants are rich source of alkaloids and steroids having prophylactic and curative properties for treatment in several diseases e.g., Aloe, Isabgol.

Aromatic plants: Aromatic plants are rich source of essential oil which is used for cosmetic industry e.g., Rose, lemon grass etc.

Mango is most important of all fruit crops in terms of are coverage and production, besides its socioeconomics providing livelihood to million of people in India and it is acknowledged as the king of fruit of this country. The greatest tribute of this esteemed plant was paid by Emperor Akabar (1556–1605) who established the Lal Bag (a mango orchards having one lakh plants) in Darbhanga (Bihar) when large orchards of fruit trees were unknown. In Ain-I-Akbari, a master piece of Persian literature, written by the great chronicler Abul Fazal (1590 AD) during the region of Akbar. Popularity of mango among the consumers is increasing due to its delicious flavour and much higher nutritional and therapeutic values. Thus, mango is now appreciated and spreading across the globe. It is grown in more than 90 countries. Washing of mouth and hand are necessary after eating the fruit so, it is also known as bathroom fruit.

Area and Production

India is the single largest producer of fruit, accounting for 49.6 per cent of area and 49.1 per cent of the total world production. Mango occupies 23.13 million hectares area in India. Amongst the major mango-exporting countries India, Mexico, Brazil and Pakistan accounted for 3.5, 1.4 and 3.3 per cent of total area under mangoes in the world respectively. The productivity of mango is highest with 13.6 tonnes/ha in Brazil and 7.0 tonnes/ha in India which is much lower. India contributed about 45.7 per cent of total mango production (35.71 million tonnes) in the world, making it the most important mango producing country. Andhra Pradesh ranks first as it produces about 27 per cent of total mango production and Uttar Pradesh is ranked second in production (23.9%) with productivity of13 tonnes/ha. At present the varieties like ‘Alphonso’ grown in Maharashtra and Gujarat and ‘Dashehari’ in U.P. are exported to countries viz. Afghanistan, Bahrain, France, Kuwait, Malaysia, Nepal, Qatar, Singapore and U.K. Mango nectar and pulp has great scope to European and American market.

Banana is an important fruit of India ranking second fruit next to mango. Its name “Adams fig”, “Apple of Paradise” and the botanical name M. paradisiaca are suggestive of its antiquity. The edible banana is indigenous to warm moist parts of Asia and probably originated somewhere in the mountainous hot tropical regions of South-East Asia (Assam, Burma, Thiland or Indo-China region). Bananas are a very important commodity for developing countries, with a clear dual nature which complicates the analysis. They are at the same time a major staple commodity, together with rice, wheat and maize, and a fundamental export commodity. Many issues are involved in order to make of banana a very sensitive commodity at the international level, not only on economical grounds but also on the environmental, social and political aspects.

Origin and History

It is believed that the earliest written reference to banana is in Sankrist and dates back to around 500 BC. Bananas are suspected to be the first fruit in the earth by some horticulturists. The origin of bananas is placed in Southeast Asia, in the jungles of Malaysia, Indonesia or Philippines, where so many varieties of wild bananas still grow at present. Bananas have later travelled with human population. The first Europeans to know about bananas were the armies of Alexander the Great, while they were campaigning in India in 327 BC. In the Middle Ages, the banana was thought to be the forbidden fruit of paradise by both Muslims and Christians. The Arabs brought them to Africa. Africans are credited to have given the present name, since the word banana would be derived from the Arab finger. The Portuguese brought them to the Canary Islands. Bananas changed during all these trips, gradually losing its seeds, filling out with flesh and diversifying. When Spaniards and Portuguese explorers went to the New World, the banana travelled with them. In 1516, when Fiar Tomas de Berlanga sailed to Santo Domingo, he brought banana roots with him. From there, bananas spread to the Caribbean and Latin American countries. Bananas started to be traded internationally by the end of XIX century.

Citrus is a major fruit crop grown all over the tropical and subtropical region of the world. Maximum citrus species are native of tropical and subtropical regions of South East Asia specially India, China in the area between these two countries. It is mainly cultivated in China, Brazil, India, USA, Mexico, Spain and Egypt in the world. China is the leading producer country in the world followed by Brazil, India and USA. India is the third largest citrus producer which has 4.8 per cent of the world’s total citrus production.

Papaya (Carica papaya L.) has emerged from the status of a home garden plant to that of commercial orchards and is popular with the farmers because it is one of the few fruits which fruits throughout the year, give quick and high returns, requires less area per tree and adapts itself to diverse soil and climatic conditions. It is an important fruit of tropical and mild subtropical regions of the country. Though India is the leading producer of this fruit, the productivity (43.27 MT/ha) is below it’s potentially (100 t/ ha). In India, Andhra Pradesh, Gujarat, Karnataka, Madhya Pradesh and Maharashtra are important centres of its production.

Origin and Distribution

Papaya is native to tropical America, from Southern Mexico through the Andes of South America. It was spread to the south by Indians, and throughout the Caribbean with Spanish exploration. It was spread in 16^th century to other parts of the world. The Spanish also carried it to Europe and the Pacific Islands. By the mid 17th century, papaya was distributed pan tropically. Papaya was introduced to Hawaii in the 1800s, and Hawaii remains the only state in the USA to produce papaya commercially. Now it is grown in all tropical and sub-tropical countries like Australia, Hawaii, Taiwan, Puerto Rico, Peru, Florida, Pakistan, Bangladesh and India. It was introduced in India in 16^th century by Portuguese. There are leading producer countries in the world which are India, Brazil, Mexico, Indonesia, Dominican republic, Nigeria, Democratic republic of the congo, Cuba, Thailand and Venezuela (Bolivarian republic).

India, is the worldwide leading producer of papaya and its principal exportation market is focused on Gulf countries. The papaya cultivation in India was 138 with production 5989 (‘000 hectare production ‘000 metric tones) in 2017–18 and it was 146 with production 6096 (‘000 hectare production ‘000 metric tones) 2018–2019. At harvested area rank, Mexico is the fifth papaya harvester, with 17,500 hectares. The maximum area in Gujarat followed by Andhra Pradesh, Kerala, and Chhattisgarh; and production is maximum in Andhra Pradesh followed by Gujarat and Karnataka, Anony, 2019.

Guava is the fifth most important fruit crop of India in area and production after mango, banana, citrus and papaya and also fifth most important fruit crop of India in area after mango, banana, citrus and apple (Anonymous, 2018). It is also called apple of tropics because it is sold at moderate prices. It is widely grown in different part of the tropics and subtropics. It is quite hardy, prolific bearer and highly remunerative crop which requires less care. Guava is an important fruit crop of India and has gained considerable importance because of its high nutritive value, availability at moderate price, a pleasant aroma and good flavour. Guava is one of the most common fruit liked alike by both the rich and the poor. Guava is a rich source of vitamin C and pectin. It is very rich and cheap source of vitamin C. The fruit is relished when mature or ripe and freshly plucked from the tree. It is also used for many preparations like jelly, cheese and juice.

Origin and Distribution

The world leading producer of Guava is India. It contributes 40.4% production of guava followed by china with 10.0% production. It is cultivated in India since early the 17^th century. Its origin is Tropical America. It has been spread throughout the tropics and subtropics. Guava is also cultivated in Israel but it is not common in the Mediterranean area and United States. However, Guava is growing widely in Southern Asia. It is cultivated in Ceylon at altitude of 1500 m and it also grown throughout Myanamar. In Florida, the first commercial guava planting was established around 1912 in Palma Sola. It is common throughout all warm areas of tropical America and in the West Indies (since 1526), the Bahamas, Bermuda and southern Florida where it was reportedly introduced in 1847 and was common over more than half the State by 1886. Apparently it did not arrive in Hawaii until the early 1800’s. Now it occurs throughout the Pacific islands. Generally, it is a home fruit tree or planted in small groves, except in India where it is a major commercial resource. In 1972, Hawaii processed, for domestic use and export, more than 2,500 tons (2,274 MT) of guavas, over 90% from wild trees. During the period of high demand in World War II, the wild guava crop in Cuba was said to be 10,000 tons (9,000 MT), and over 6,500 tons (6,000 MT) of guava products were exported. Brazil’s modern guava industry is based on seeds of an Australian selection grown in the botanical garden of the Sao Paulo Railway Company at Tatu.

Grape was known to grow in India from ancient time but its commercialization is relatively recent, with the discovery of, Anab-e-Shahi’ variety by Mr. Shanker Pillay in 1930 from the bungalow of Nawab Baquer Ali Khan. This variety performed very well in the Hyderabad region of Andhra Pradesh and in fact gave initial boost to the Indian viticulture. It was initially known as ‘Malta’ and proved to be a pioneer in revolutionizing grape industry in south Indian.

Grape growing in north India received impetus from its success in south Indian. The commercial grape growing in north India dates back to early sixties when the ‘Pusa Seedless’ variety was released by the Indian Agricultural Research Institute, New Delhi. Latter on, the Punjab Government imported 100,000 cuttings of early-maturing variety ‘Perlette’ from California (USA). This variety acclimatized well and proved good for commercial cultivation.

In western Rajasthan the grape was introduced around 1960, which became popular among the growers. The fruit yield declined slowly thereafter and the grape cultivation was almost abandoned by 1971–72, particularly in the arid district. The problem of the drop of flower and flower-bud has been over of the major constraints in grape growing in western Rajasthan.

Pomegranate is one of the important fruits of tropical and sub tropical regions. It is also cultivated in semi arid and arid tracts of the country but requires irrigation facilities for better quality of fruits production. Pomegranate is used as fresh and making delicious juice. In India, it is cultivated for domestic consumption and export. Versatile adaptability, hardy nature, less cost in orchard management, low irrigation water requirement as compared to other commercial fruit crops, high yield potential, fine table and therapeutical values and excellent keeping quality has made this fruit more lucrative and remunerative (Sharma et al., 2014).

In global market, India plays a significant role of export of fruits. Recently exports of pomegranate from India have increased and extended to several countries. India stands firmly at third position with 14% market share in pomegranate export after China and Iran.

The annual export of pomegranate fruits from India is about 67, 892 metric tonnes (MT) amounting to revenue of ‘688 crore (Annon, 2019). For export purpose, Phule Arakta, Bhagawa, Super Bhagawa and Mridula varieties are suitable (Anon, 2003).

The highest importer of Indian pomegranate is Gulf countries led by United Arab Emirates (19,000 MT) followed by South East Asian Countries prominently, Bangladesh (10,500 MT) and Nepal (10,000 MT). Among European countries the biggest exporter is Netherlands (4,449 MT) followed by UK (576 MT). Recently, India has also started exporting fresh pomegranate to USA to the tune of 209 MT generating a revenue of 11 crore ((Annon, 2019).

It is tropical fruit which is commercially cultivated in humid areas of India. Fruits are very tasty, sweet and rich in sugar. Trees are ever light green, slow growing and it attains a height of about eight meters.

Origin and Distribution

Sapota is native to Central and South America, specifically from the Yucatan Peninsula of Mexico to Costa Rica, where the largest population of native trees still exists (Gilly 1943). It is now widespread throughout the tropical regions of the world, including Central and South America, the West Indies, India, and Florida in the United States.

Sapota is grown on a commercial basis in India, the Philippines, Sri Lanka, Malaysia, Mexico, Venezuela, Guatemala, and some other Central American countries. India is the largest producer of sapota fruit with current production around 24,000 ha (Chadha 1992). Sapota is widely planted in south Florida, where the fruit is marketed locally and shipped to northern and eastern U.S. markets. The fruit, however, is not commonly seen in the United States. In southern Mexico and Central America where sapodilla is native, it is considered to be one of the best of the tropical fruits.

Medicinal plants especially underutilized fruit are the nature’s gift to arid zone. Many of fruits are popular in this area but aonla posses an important place among all. Aonla or Indian gooseberry (Emblica officinalis Gaertn) is native to tropical south-eastern Asia mainly peninsular of central and southern India. The tree is known as Amla, Amlaki, Amli, Ambala, Amalakamu and Nelli in different parts of India. It is a very hardy tree and can be grown under diverse soil and agro-climatic conditions without much care. Aonla fruits has been reported to have potential of antioxidant, free radical scavenging capacity, anti-inflammatory, anti-mutagenic, immune modulation properties, which is used to cure various cancer, diabetes, liver and heart related diseases

Aonla has been regarded as a sacred tree in India. It has religious value and it is medicinally very important since ancient time. It has hallowed position in Indian medicine system called as Ayurveda as a protective remedy for several mankind problems. The tree was worshipped as Mother Earth and is believed to nurture humankind because the fruit are very nourishing. Kartik Mahatma and Vrat Kaumudi order the worship of this tree. The leaves are offered to the Lord of Shri Satyanarayana Vrata, Samba on Shri Shanipradosha Vrata and Shiva and Gowri on Nitya Somvara Vrata. The fruit and flowers are also used in worship. Fruits of aonla are processed in various forms like Juice, Murabba, Chayvanprash, candy, powder and flakes which has significant role in mitigating several malnourished disorders. Aonla cultivation has gained special attention of researcher community due to its significant role in medicinal industry as well as processing potential.

The ber is an important indigenous fruit and growing in semi arid conditions in the country. Ber is grown in China for the last 4000 years and under sub tropical to tropical as well as Mediterranean regions of the world. It is cultivated widely for its resistance to grow in drought and other diversified climatic and soil conditions, so it is also known as ‘King of fruit’ of arid.

Origin and Distribution

The origin of ber is said to be India to South-western Asia. The ber is being cultivated in Northern India, central India and peninsular India. Hisar, Rohtak, Panipat, Gurgaon, Jind, Mahindergarh in Haryana. In Rajasthan it is cultivated in district of Bharatpur Alwer, Barmer, Jodhpur, Palib and Gnaganagar Jaipur in area 82, 73, 59, 53, 53, 53,41 hectare with production 365, 395,133, 294, 340, 597, 826 metric tones, respectively. However total cultivated area in Rajasthan is 738 hectare with production 5248 metic tones under ber during 2017–18 (Anonymous, 2018).

The area Sangrur and Patiala in Punjab; Banaskantha and Sabarmati in Gujarat; Bijapur and Bellary in Karnataka; Tirunalveli, Ramanathapuram and Salem in Tamil Nadu are major ber growing areas in the country. Besides India, ber is grown in Afghanistan, Iran, Syria, Burma, Australia, USA, Russia, Italy, France, Spain and Northern Africa.

Date palm (Phoenix dactylifera L.) is an important fruit tree of semi-arid and arid regions. It grows well under poor desertic soils due to its hardy plant characteristics and deep root system. It is also one of the most suitable fruit trees, which can be easily grown under saline conditions. In arid region, crop production is risky preposition, where date palm cultivation contributes in achieving food security, high nutritive value food, crop diversification, desertification control, income generation and foreign exchange earnings. It can be grown at such places where adequate irrigation facilities are available besides other climatic requirements.

The latin name of the date palm, Phoenix dactylifera is derived from a Phoenician name “Phoenix”, which means date palm and dactylifera derived from a Greek word “daktulos” means a finger. In India, generally two species of Phoenix are found. Out of which, Phoenix sylvestris Roxb. called “desi khajoor” is found throughout the country but it produces inferior quality fruits. The fruit contains large stone with less pulp content. From its crown parts, a liquid is extracted known as neera or taddi, which is used as fresh drinks and also for preparation of jaggary. The tree of wild date/ desi khajoor (Phoenix sylvestris) is found in Bundelkhand region of the country and it is suitable tree component for agro forestry system (Tewari et al., 2001). Phoenix dactylifera is commercially grown for edible fruits and value added products.

Litchi (Litchi chinensis Sonn.) is a subtropical and evergreen tree belonging to the family Sapindaceae. This fruit has highly specific climatic adaptability due to chilling requirement for floral initiation on one hand and the damage caused due to high summer temperature along with low relative humidity during fruit growth period on the other hand. Some other factors influencing expansion in different regions of the world are difficulties in establishment of plants in nursery and orchards, bearing and further into harvesting and post-harvest management. Calcium rich soil with mycorrhizae is best suited for the luxuriant growth of litchi plants which are commercially propagated by air-layering or gootee.

There has been a substantial increase in the area and production of litchi in India during the past 50 years. The area has increased from 9400 ha (1949–1950) to 91,000 ha (2016–2017). The contribution of litchi to the total area under fruit cultivation has increased from 0.75% to 1.70%. Increase in area during 1991–1992 to 2016–2017 (22 years) has been more than 80%, whereas production increase during the same period is to the tune of more than 150% (NHB, 2016–17). Productivity also recorded an increase of about 50% during the same period. Evidently, production and productivity of litchi are constantly increasing in the country. On a global scale, the fresh fruit market dominates the trade, followed by dried and canned fruit. Low success rate of establishment, lesser fruiting span, low and irregular yields because of poor flowering and fruit set, fruit cracking, browning and rotting of fruits, fruit borers and mites, poor shelf life, and lack of suitable varieties with early and late maturity and good-quality fruits are some of the factors encountered

Custard apple (Sitaphal) is one of the most delicious fruit. The genus name, ‘Annona’ is comes from the Latin word ‘anon’, which means ‘yearly produce’ has three species namely Annona squamosa, Annona cherimola and Annona reticulate. Out of which Annona squamosa has been extensively used as fruit crop and traditional medicine for different ailments. The seeds and the leaves also are being used for preparation of medicine. It is hardy, tolerant to drought, salinity and saline irrigation water to certain extent. It grows very well in shallow soil profile. It also sheds off leaves during stress condition to evade moisture loss from plant tissue through transpiration. This crop is known by varied name like sitaphal, shariffa and sugar apple.

Origin and Distribution

The custard apple is believed to be a native of the West Indies but it was carried in early times through Central America to southern Mexico. It has long been cultivated and naturalized as far south as Peru and Brazil. It is commonly grown in the Bahamas and occasionally in Bermuda and southern Florida. Apparently it was introduced into tropical Africa early in the 17th century and it is grown in South Africa as a dooryard fruit tree. In India the tree is cultivated, especially around Calcutta, and runs wild in many areas.

The bael (Aegle marmelos (L.) Corr.) is indigenous to India and it is also called as Baelpatra, Bengal quince (John and Stevenson, 1979), Indian quince, golden apple, holy fruit, bel, bilwa, bela, maredoo and other dialectal names in India. Hindus used the leaves to worship Lord Shiva (Bilwa Patra) and keep them on Shivling and they consider it is very auspicious and to get the blessings of Shiva. The fruits form a vital ingredient of holy offerings to the God and Goddess through holy pyre (haven). In history, the mention of bael tree is traced to Vedic times (C 2000 BC. to C 800 B..) within the Yajur Veda.