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Biodiversity is the variety of life in different microbes, animals, plants and their genes present in the ecosystem. Soil provides a vital habitat and environment primarily for microflora such as bactria, actinomycetes, fungi as well as microfauna viz. protozoa and nematodes, mesofauna such as microarthopods, enchytraeids, macrofauna such as earthworms, beetles, termites and millipedes (Gaur 2006a).

Biodiversity and soil are interlinked which interact closely with wider biosphere. Conversely, biological activity is ofprime importance in influencing the physical and chemical conditions of soil (Bardgett 2005). The level of soil biological activity is, therefore, affected by soil type and also depends on the use of management practices which contribute to crop productivity.

Soil biodiversity and soil processes

A majority of soil organisms participate in various processes which are essential for the biosphere. The main ecological function is to decompose organic matter largely derived from the plant and animals residues Which consist of celluloses, hemicelluloses and lignin, and to supply plant nutrients to support growth. These organisms participate in various nutrient cycles and processes such as nitrogen mineralization, nitrification, nitrogen fixation, transformations ofphosphorus, potassium, sulphur, calcium, magnesium, iron and micronutrients (Pankhurst et al 1997). They participate in bioremediation of harmful compounds such as heavy metals and pesticide residues (Gaur 2006 b). They not only maintain physical and chemical conditions of soil, but also regulate emissions / assimilations of green house gases and suppress soil borne diseases and pests

Introduction

In poultry, the intestine is home to trillions of microorganisms, with bacterial concentrations reaching up to 10¹² bacteria per gram of digesta. In a healthy digestive system, this microbial community is highly diverse and metabolically active, supporting crucial functions in digestion, immune defense, and overall health. The gastrointestinal tract (GIT) of a newly hatched chick begins as a sterile environment but quickly develops a complex microbiota as the bird matures. The composition of gut microbiota is highly variable, shaped by environmental factors, diet, the genetic makeup of the host, and other influences. This dynamic microbiome plays a pivotal role in nutrient absorption, disease resistance, and growth efficiency, making it a central focus in poultry health management and productivity enhancement.The health and productivity of poultry are fundamentally influenced by the gut microbiome, which shapes digestion, immunity, and growth potential. Microbial diversity variesacross several poultry species, including broilers raised intensively, native chickens in free-range conditions, and unique species such as Guinea fowl, Kadaknath, and red jungle fowl. Using advanced metagenomicsanalysis of microbial diversity, structure, and function, highlighting the implications of microbiota composition on poultry health and productivity possible. The gut microbiota is increasingly recognized as a major determinant of health and productivity in poultry. It not only influences nutrient absorption and metabolism but also plays a critical role in immune response and pathogen resistance. Intensive farming practices have altered gut microbial ecosystems in commercial broilers, often reducing diversity compared to native breeds and heritage species raised in field conditions.

The traditional fermentation process is an uncontrolled system and is usually carried out in a quite unhygienic environment. As the process of fermentation is brought about by the microbial activity so in the traditional system of fermentation there is every possibility of inclusion of microorganisms which is not desired in or that can cause spoilage to the finished product. Although no incidents of food poisoning has been reported due to consumption of fermented fish there are also no reports of any clinical investigations in this regard. A study on the microflora of such a fermented fish product is useful to understand -how safe is the product for consumption. The diverse groups of microorganisms that are present in the fermented fish products will provide information about their characteristic nature -beneficial or pathogenic, their contribution towards production of flavour and aroma, etc. The environment, however, is the key factor for the so called ‘microbial diversity’ among different fermented fish products round the globe. Moreover, the aquatic habitat, processing technique and of course, the handling during processing are the other factors that equally make a way for the introduction of a variety of microorganisms in the finished product. A study on different curing stages i.e. fresh, semi-cured and fermented, is therefore necessary to understand the pattern of microbes and become evident the role played by the microbes during fermentation.

1.    Introduction    129
2.    Soil Microbiology    129

    2.1.    Bacteria, 130
    2.2.    Fungi, 132
    2.3.    Algae and Cyanobacteria, 132
    2.4.    Protozoa, 132
    2.5.    Viruses, 135

3.    Water Microbiology    133
4.    Microbial Associations    133

    4.1.    Neutral interactions, 133
    4.2.    Positive interactions, 133
    4.3.    Negative interactions, 133

Several attempts have been made to detect the biopotentials of microorganisms with reference to their ability to produce various enzymes with a view to use them in industrial processes and bioremediation applications. Enzymes are increasingly used as a new route to synthesize food ingredients such as flavours, emulsifiers, sweeteners, food additives, nutrients. With the advent of genetic engineering, a wide range of enzymes has become available on a large scale and this has increased the scope of enzyme technology.

MICROBIAL ENZYMES: Enzymes are proteinaceous complex organic catalysts of biological origin and vital constituents of cells. Microbes produce a variety of enzymes, intracellular and extracellular enzymes. Most of the microbial enzymes produced for commercial usage are extra cellular enzymes. Extracellular enzymes are secreted outside the cell and hydrolyze polymeric substances. Amylases and proteases are enzymes of economic importance. At present over 60 enzymes are in commercial use, of which three-quarters are hydrolytic enzymes. Commercial production of microbial enzymes utilizes a variety of fungi, yeasts and bacteria. The composition of the medium, genetic stability of the microbial strain and physical and chemical factors of fermentation influence the production of a particular enzyme.

Enzymes catalyze a variety of reactions during processing and preservation of food products and form a large group of processing aids. Food industry utilizes enzymes in the production of bakery items, dairy products, fruits and vegetables juices and products, tenderization of meat, modification of oils and fats, flavour production and preservation of food products. Enzymes have also found use in the manufacture of ingredients like glucose, high fructose syrup, citric acid, colour and flavour constituents.

The study of soil microorganisms involves various complicacies when compared with those of plants or animals aboveground. The complicated heterogeneous environment existing in soil and the varied growth behaviour of microbial populations in soil have necessitated the development of special techniques to study the microbes and their metabolic diversity, individually and holistically without disturbing their natural habitat. With the gradual improvement in microscopy since its discovery in 1667 and also with the greater understanding of the nature of soil microbes and their properties in relation to total ecosystem function, the concept of studying microbes in isolation has enormously changed. Microscopes have helped in magnifying these, otherwise invisible, minute creatures within the limits of naked eyes.

SINGLE CELL PROTEIN (SCP) AND PROTEIN DEFICIENCY

Protein is an essential component of diet. The greatest single problem in the world today is global food protein shortage. Having adequate quantity of food is only one side of the picture, but the most important aspect is the supply of balanced and nutritious food. All efforts are being made to increase the food production through modern methods of farming. But very little attention is being paid towards protein requirements and production. Approximately half of world population suffers from the protein deficiency disease called ‘Kwashiorkor’ and children with poor protein are victims of this disease.

Usually, cereals have low protein values while protein rich pulses are not available in desired quantities due to poor yields and high costs. The good quality protein available in fish, meat, poultry, and eggs is not available due to poor economic condition as well as varied dietary habits. With the current system of production, agriculture cannot be relied upon to feed an ever increasing world population. Hence there is an urgent need to find other protein sources. The best potential is seen in microbial protein or single cell protein (SCP), a new source of protein independent of agriculture.

DEFINITION: The dried cells of microorganisms such as bacteria, fungi, yeasts, algae that are grown in large scale culture systems as proteins for human or animal consumption are collectively known as "single cell proteins".

Introduction

Hippocrate, an ancient physician, a great thinker and also often referred as father of medicine, for the first time conceive the idea nearly 2,500 years ago that a disease is caused due to natural factors such as environmental factors, diet, and living habits. He advocated the secret of healthy living in healthy food in his famous quote “Let food be thy medicine and medicine be thy food”. But unfortunately, this wisdom about food has faded with the time and we became dependent on synthetic medicines and drugs for even life style disorders. In the last century, research advancement in the field of food science and nutrition established food not only as a source of nutrition but also a source of active food components that aid specific functions and rejuvenated the Hippocrates concept. According to the Department of Health and Human Services, USA, daily diet of a person, plays a crucial role in 5 of 10 diseases which are the leading causes of death, such as diabetes (noninsulin dependent or type 2), stroke, coronary heart disease (CHD), specific type of cancer, and atherosclerosis. In the mid-1980s, term “functional food” was introduce in Japan and defined as food that contain active components either from plant or animal origin and provide physiological benefits in addition to the basic nutritional needs.

Genetics is the study of what genes are, how they carry information, how their information is expressed, and how they are replicated and passed to subsequent generations or other organisms.

DNA in cells exists as a double-stranded helix; the two strands are held together by hydrogen bonds between specific nitrogenous base pairs: A-T and C-G.

A gene is a segment of DNA, a sequence of nucleotides, that code for a functional product, usually a protein.

When a gene is expressed, DNA is transcribed to produce RNA; mRNA is then translated into proteins. This is also referred as Central Dogma of Life. The DNA in a cell is replicated before the cell divides, so each daughter cell receives the same genetic information.

The term growth for bacteria and other microorganism usually refers to changes in the total population rather than an increase in size or mass of an individual organism. As the mechanism of conservation and dissipation of heat generated during metabolism lacks in bacteria, the enzyme system in bacteria gets directly influenced by temperature. The purpose of studying the bacterial growth curve is a) to become familiar with the population growth dynamics of bacterial culture and b) to plot a growth curve and determine the generation time of bacterial culture.

A number of methods are now available for measuring bacterial growth. Some important ones are

1)By colony counting or cell counting

2)By weighing the cells

3)By cell activity (turbidity method)

Abstract

Discovery of the agricultural utility of microbes during the later part of the eighteenth century, led to the formulation and utilization of superior microbial strains as biofertilizers, for a wide variety of crops and growing conditions across the world. Though microbial inoculants have been in usage for almost two centuries, they are yet to capture the collective imagination of farmers across the globe, which is essential to meet the larger objective of reducing chemical input usage in cropping systems. One probable reason for this is the existence of large growing tracts that are subject to various abiotic stresses of atmospheric and edaphic origin, which limit the proliferation and efficiency of the microbial strains that are commonly used in inoculant production.  This is obvious, since unlike chemical fertilizers, microbial cells being living systems need to grow and thrive in the introduced eco-systems in order to achieve the desired effects of inoculation. But unfortunately microbial inoculant technology has largely adopted the one size-fit all mindset, which had led to failures of microbial inoculants especially in abiotically stressed growing environments. In such a scenario it would be wise to adopt a location or region specific approach for the selection of superior and locally adapted microbial strains for inoculant production.

Introduction

The increasing concern about the long-term productivity of agro ecosystems has emphasized the need to develop a management strategy that maintains and protects the soil resources. This is directly related to the maintenance of the level of soil organic matter and nitrogen status, which are the critical components of soil productivity. These parameters can change over time due to crop management practices such as cultivation, crop rotation, residue management or fertilization which, in turn, can influence the long-term sustainability of ecosystem. Application of chemical fertilizer is one of the most expensive inputs in agriculture. About 65% of the applied nitrogenous fertilizer is lost from the plant–soil system through gaseous emissions, runoff, erosion and leaching which causes greenhouse effects and nitrate pollution of surface and ground water (Rejesus and Hornbaker 1999). Similarly, P applied in soil is rapidly fixed and results in low P availability for plant production. In this context, the deployment of microbial biofertilizers promise increasing soil nutrient availability in integrated nutrient management system.

The increase in population and global industrialization has led to environmental pollution, which is a significant threat to the health of the ecological system. Different measures are recommended to remediate polluted areas effectively. The applications of nanotechnology in medicine, cosmetics, sports, textiles, and pharmaceuticals have increased over the years. Another approach to remediate the adverse effects of pollutants on the environment is by utilizing nanotechnology for contaminant remediation. Biogenic nanoparticles are tiny particles produced by biological entities such as fungi, bacteria, yeast, and algae. These biogenic nanoparticles are efficient in removing pollutants like heavy metals, pharmaceutical waste, dyes, and hydrocarbon waste. 

1.    Introduction    174
2.    The Thermodynamic and Mechanistic basis of Cellular Metabolism    174

    2.1.      Adenosine triphosphate, 175
    2.2.    Coenzyme A, 176
    2.3.    Modes of ATP synthesis, 177

3.      Components of Electron Transport Chain    181

    3.1.    Electron carriers in ETC, 182
    3.2.    Complex I - NADH dehydrogenase (NADH coenzyme Q reductase), 185
    3.3.    Complex II - Succinate - coenzyme Q reductase, 187
    3.4.    Complex III - Coenzyme Q - cytochrome c reductase, 187
    3.5.    Complex IV - Cytochrome c oxidase, 188
    3.6.    ATP synthese, 188

The essential nutrients and their function in microbial cells

1. INTRODUCTION

Flavour is a distinctive property of a substance affecting the gustatory sense. It may be defined as “The sum of those characteristics of any material taken in the mouth, perceived principally by the senses of taste and smell, and interpreted by the brain.” Flavour perception is a complex process which requires the particles to be mixed with saliva and salivary enzymes to promote the release of flavour. Flavour molecules are very diverse in nature. More than 5000 compounds in food have been identified as flavour components which can be detected at very low concentration and are thus, used in products at very low levels. Hexenols and hexenals, characteristic of green taste of fresh fruits and vegetables, can be perceived at a concentration of about 1 part per million (ppm). Naturally occuring flavour compounds are present at a very low concentration ranging from parts per trillions (ppt) to parts per millions (ppm) level. Majority of these compounds are volatile, with boiling points in the range of 20-300 °C. Most of these compounds are lipophilic and possess a great diversity of chemical group and structure with molecular weight ranging between 50-250 g/mol.

Flavour and fragrance compounds are a part of expanding industry with an estimated cost of nearly $27 billion. These comprise of diverse molecules with unique structures and functional groups that contribute to varied aroma and flavour, ranging from floral to fruity notes. The processing operations modify the equilibrium between the different components in raw food that might weaken the original flavour, which require subsequent supplementation. These flavour supplements can be natural or chemically synthesized. Of all the available flavour compounds, around 80% are produced by chemical synthesis whose use is limited due to formation of racemic mixtures and lack of substrate specificity. The growing awareness of consumers towards chemicals supplemented to food has led to the development of the flavouring compounds of biological origin referred to as natural flavours or bioflavours. The first major source of flavour compounds was primarily from plants. Identification of microbes producing vital food flavourants paved the way for alternative low cost, efficient and flexible method for the commercial production of flavour compounds.

Abstract
 
Opencast mining destroys the original land ecosystem and microbial community due to mining and stockpiling of mine rejects on adjoining land, which is a matter of great environmental concern. Mining creates formation of wastelands, formation of gullies, ravines, overburdens, reduced water infiltration and increased runoff. Reclamation of mined out areas is often difficult due to its chemical, physical and biological traits. Absence of topsoil is the most common feature of the mine spoils dumps. Arbuscular mycorrhizal fungi (AMF), the synonym of VAM fungi, are important microbes of soil that form symbiotic association with most of the terrestrial plants on the earth. Observations led to use of legume microsymbionts as biological substitutes for fertilizers. AMF diversity increases with species diversity of plants as the potential number of association increases. Dominant arbuscular mycorrhizal fungi can prevent the invasion of non-mycorrhizal plants on land where they have established symbiosis and promote their mycorrhizal host.

Introduction

Bacteria are very minute and colourless in their natural environment and are difficult to be seen under compound microscope. To view their exact morphology, various kinds of stains are used. Stains are of three main types.

Acidic stains: These are those stains in which charge on dye ion is negative. e.g. India Ink, Eosin etc.

Basic stains: These are those stains in which charge on dye ion is positive. e.g. Methylene blue, Crystal violet etc,

Neutral stains: These are the salts of dye acid in dye base. e.g. Eosinate of Methylene blue.

Abstract

Anaerobic sulfur compound reduction processes make use of sulfur, thiosulfate, trithionate, tetrathionate, and sulfite during the reduction process. Complete reduction of these sulfur compounds leads to production of H₂S. Isolation of sulfur compound reducers requires a suitable electron donor and one of the sulfur compound as a sole electron acceptor during the reduction process carried out in anaerobic conditions. Anaerobic sulfur, thiosulfate and tetrathionate reductases identified to date are molybdopterin binding oxidoreductases that extract out electron during the reduction process. The electron transport chain during the reduction process involves cytochromes and menaquinones. Dissimilatory sulfite reductases include conventional siroheme dissimilatory sulfite reductase and the nonconventional sulfite reductases including ASR sulfite reductase, F₄₂₀ dependent sulfite reductase and the MccA sulfite reductase. Anaerobic sulfur compound reduction process are almost ubiquitous in nature and are important part of the biogeochemical cycle. They occurs in aquatic systems, are found in extreme environments such as hydrothermal vents, acid mine drainage sites and are also found in important human and animal pathogens.

Abstract

Microbiological synthesis of nanoparticles has emerged as rapidly developing research area in nanotechnology across the globe with various biological entities being employed in production of nanoparticles constantly forming an impute alternative for conventional methods. Simple prokaryotes to complex eukaryotic organisms including higher plants are used for the fabrication of nanoparticles. In the present article, the synthesis of important plant nutrients like Mg, Zn, Ag, Au, Ti, P and Fe nanoparticles from different fungal species, their characterization and application potential of nanonutrients in agriculture is elaborately discussed.

Introduction

A toxin (Greek: ôïîéêüí, toxikon, lit. (poison) for use on arrows) is a poisonous substance produced by living cells or organisms that is active at very low concentrations. Toxins can be small molecules, peptides, or proteins and are capable of causing disease on contact or absorption with body tissues by interacting with biological macromolecules such as enzymes or cellular receptors. Toxins vary greatly in their severity, ranging from usually minor and acute (as in a bee sting) to almost immediately deadly (as in botulinum toxin)

Soil microorganisms immobilize and mineralize various elements to satisfy their growth requirements. The subsequent turnover of microbial biomass also releases several mineral elements. The elements may be released as a result of organic matter decomposition (e.g. during the process of mineralization, ammonification, etc.) or through biological weathering. Other transformations may involve oxidation and reduction of various inorganic ions and compounds which are mostly catalyzed by chemoautotrophic bacteria during oxidation of ammonium, nitrite, sulphur, iron, etc. Such oxidations may also release energy for microbial growth.

Introduction

Phosphorus is only second to nitrogen as a mineral nutrient required for plants, animals and microorganisms. It is a major constituent of nucleic acids in all living systems essential in the accumulation and release of energy during cellular metabolism. Phosphorus is found in soil, plants and microorganisms in number of organic and inorganic compounds. It is the second major inorganic nutrient after nitrogen in term of quantitative requirement for both crop plants and microorganisms. This element may be added to soil in the form of chemical fertilizers, or it may be incorporated as leaf litter, plant residues or animal remains. In cultivated soils it is present in abundance (i.e. 1100 kg/ha), but most of which is not available to plants, only 15% of total soil phosphorus is in available form. Thus, phosphorus occupies a critical position both in plant growth and in the biology of soil.

Introduction

In soil, microorganisms influence not only the availability of carbon and nitrogen, which are important constituents of plants, but also of sulfur (S), phosphorus and many other trace elements for plant absorption. Sulphur is found widely distributed and considered as tenth most abundant element in the nature. Sulfur is considered as an essential element for survival of plant and animals and found as gypsum (CaSO₄.2H₂O), pyrite (FeS₂) and other combined form and also in elemental form (S⁰) in the nature. The requirement of sulphur by plant is comparatively less than the primary nutrients, hence sulfur is recognised as ‘secondary’ nutrient. 

The planet earth harbors a total population of 7,530,687,482 (as on 7^th September 2017 at 1345hrs) in which Asia and Africa share 4,486,207,286 and 1,252,251,830, respectively. In majority of the countries in Asia and Africa surveillance programs for detection and source tracking of human health hazard bacteria in foods is scant and in some cases do not exist. Hence, the task is much more complicated especially catering to needs of food, nutritional security and microbial safety of foods of more than 5,738,459,116 or 76.2 % of the total population of the world. The continued occurrence of foodborne illness is not evidence of the failure of our food safety system. In fact, many of our prevention and control efforts have been and continue to be highly effective. In advanced countries like US where food supply is one of the safest in the world, however, significant food borne illness continues do occur. Despite great strides in the area of microbiological food safety, much remains to be done.

Food-borne disease outbreaks are defined as the occurrence of 2 or more cases of a similar illness resulting from ingestion of a common food or observed number of cases of a particular disease exceeds the expected number. These can be confirmed (when at least one causal agent is identified) or suspected (based on clinical and epidemiological information). Although most cases are sporadic, these diseases draw attention to themselves due to outbreaks, thorough investigation of which can help in identifying control measures.Annual burden of foodborne diseases in the WHO South- East Asia Region includes more than: 150 million illness, 175 000 deaths, 12 million DALYs (Source: FERG Report 2010).

Introduction

Numerous characteristics of food determine its acceptability to consumers and to regulatory officials. Before accepting a food for consumption, it must be attractively presented, must have desirable odour and flavour characteristics and other qualities and must be free from potential pathogens or their toxic products. The ultimate nutritive qualities, flavour and appearance have been preserved and no harmful organisms or substances are present to affect the consumer adversely. If the quality of the product is good, its keeping quality is also likely to be substantial. Conversely, there are more chances of spoilage of an inferior quality food product during storage.

1. Introduction

Fruits and vegetables have been an integral part of man’s diet from immemorial times. Fruits are the portions of plants that bear seeds, while vegetables are the edible components of a plant, including the leaves, stalks, roots, tubers, bulbs, flowers and seeds. These can be eaten raw or cooked. Fruits and vegetables can be preserved for short-term intervals under refrigeration, with or without receiving a minimal treatment like slicing, chopping, shredding or peeling. Fresh produce may also be irradiated or packaged in controlled or modified atmosphere in films for short-term storage. Long-term preservation includes canning, juicing, drying and addition of preservatives. Food contamination with hazardous biological agents remains a worldwide challenge in food safety and nutrition (Kenny 2002; Schlundt 2002; Tauxe et al 1997). Microorganisms form part of the epiphytic flora of fruits and vegetables and many will be present at the time of consumption. Production practices, growth conditions and the location of the edible part during growth (soil, soil surface, aerial part) will in combination with intrinsic, extrinsic, harvesting and processing factors affect their microbial status at the time of consumption. Man-made sources may be directly from farm laborers and from the equipment used for harvesting or storage. The types of microorganisms on fruits and vegetables would also vary with the orchards, vineyards or growing fields. The resident microflora normally subsists on the slight traces of carbohydrates, proteins and inorganic salts that dissolve in the water exuding from or condensing on the epidermis of the host. The numbers of bacteria present will vary depending on seasonal and climatic variation and may range from 104 to 108 per gram. The inner tissues of fruits and vegetables are usually regarded as sterile (Lund 1992). However bacteria can be present in low numbers in the insides of the fruits and vegetables as a result of the uptake of water through certain irrigation or washing procedures.

In the last decade or so, the problem of determining the microbial content and their activities in dairy products has been under close scrutiny. The dairy industries still adopt the dye reduction test because it is cheap, simple and satisfactory. However, other new techniques have been introduced along with some modifications in traditional tests. The microbiological quality must be maintained. To assess the bacterial content and their activities in dairy products, various methods are available for the microbiological testing of milk. The specific tests should be chosen as per the application and other factors like cost etc. On the basis of public health and economic consideration it is necessary to test microbiological quality of milk at a number of points along the chain from producer to consumer which includes - raw milk supply, bulk milk arriving at processors premises, monitoring bulk milk during storage prior to processing and monitoring the procession & the quality control of finished products. The techniques for estimating bacterial numbers in raw material are divided into two categories.

Introduction

In this microbiology report, we delve into the intricate realm of bacterial isolation and identification, exploring the fundamental processes that unlock the mysteries of microbial worlds. From the methods employed for isolating bacterial strains to the intricate techniques of identification, this report aims to provide a comprehensive understanding of the crucial role microbiology plays in unraveling the secrets of these microscopic organisms. Join us on a journey through the principles and applications that form the backbone of bacterial isolation and identification in the fascinating field of microbiology.

1. Bacteria intoxication - already formed bacteria not live bacteria

2. Bacterial infection consumption of live bacteria

3. Neuro toxin produced by C. botulinum

4. Listeria identified by the serotyping

5. Fishery product have type E, C. botulinum

6. S. aureus produced entiro toxin

7. S. aureus is heat resistant but not active in pasteurization

8. E. coli-0157:H7

9. Salmonella should be below 105/g

10. Septicemia can occur due to the salmonella

11. L. monocytogens should be 10/ g

Microorganism is a collective term for all living organisms, which are not visible to the eye, and occupy an intermediate position between the vegetable and animal kingdoms. They are found every where in the atmosphere, in water and in the soil and, as they break down organic materials, they play a very important role in the cycle of the nature.

There are thousands of species which are important to the life and economic structure of the human race. For example, certain species form simple chemical elements during the break down of dead organic material which plants can use again. They increase soil fertility and crop production which results in more food.

Other species are present, and essential, in animal intestines, helping in the digestion of food.

Some microorganisms are used in many food processes, e.g. dahi, yoghurt, cheese, pickles, beer and wine as well as in the acid production to preserve foods. There are microorganisms that produce toxic substances which kill other organisms, e.g. the mould penicillium which produces the substance penicillin.

Many microorganisms cause diseases in animals and plants with a subsequent reduction in the nation’s food supply, whereas others cause food deterioration such as mouldy foods, tainted foods, etc.

The basic methods for wastewater sewerage (collection, treatment, and disposal) are as follows:

• Off-site sewerage
• Separate sewerage system
• Combined sewerage system
• On-site sewerage

Many nations have implemented separate sewage systems that transmit sewage and storm water through separate pipelines in order to separate the two. Storm water often does not contribute to a wastewater treatment facility (WWTP). Domestic sewage contains 99.9% or more water. The remaining portion is made up of bacteria

Life on Earth is dependent on microorganisms for many essential services. Microbes are intimate partners in global agriculture by way of mobilization of nutrients, antagonism against pathogens and exploitation of plant- microbe interactions and optimization of plant microbiome allows farmers to apply less chemical fertilizers and pesticides, and improving plant growth and yield for sustainable development.

India has 2.4% of land area of world and 8.1% global species diversity. India has 7500 km long coast line in nine states and three union territories. About 14% of Indian population (17 crores) lives in coastal area. In 10.78 million ha of coastal ecosystem, rice is the major coastal crop. Coastal rice accounts for 15% of total area and production of India. Coconut, cashew, areca nut, spices, plantation and fruit crops are the other important coastal crops. The east coast in four states (Tamil nadu, Andhra Pradesh, Orissa, West Bengal) and one union territory (Pondicherry) has sandy coast and plains with medium to high rainfall (100-250 cm), and hot humid climate. It has marshy lands and mangroves. The west coast in five states (Kerala, Karnataka, Goa, Maharashtra, Gujarat) and two union territories (Diu and Daman, Dadar and Nagarhaeli) has fertile sandy plains with high rainfall (250-300 cm) and hot humid climate suitable for horticulture and plantation crops. Western Ghats near west coast is a hot spot with high biodiversity. ICAR established the Central Coastal Agriculture Research Institute (CCARI) at Goa in 2015 to carry out research on coastal agricultural crops.

Soil salinity in coastal regions is not only a soil type character, but also due to low quality irrigation water. The plant associated microorganisms “Plant microbiome” play a significant role in conferring resistance to soil salinity. The endophytic bacteria- rhizobium, rhizobacteria- azospirillum, azotobacter, phosphobacteria, PGPR bacteria, cyanobacteria, symbiotic fungi- ecto and endo mycorrhiza, alleviate the impact of soil salinity. Minimal microbiome for plants is the key determinant of plant health and productivity. The plant- microbe interactions are of three types: (i) Positive: Beneficial organisms- nitrogen fixers, phosphobacteria, mycorrhiza; (ii) Negative: Harmful organisms - plant pathogens; (iii) Neutral: Transient organisms. Millions of microbes inhabit plants, form complex ecological communities to improve plant growth, crop quality and productivity.

Abstract

Cow and buffalo milk is the major source milk worldwide contributing 81 and 15% of total milk production respectively. However, milk from animals like goat, sheep, camel, donkey, yak etc also contribute to human nutrition and health in different parts of world. These milks are source of staple traditional product and considered as delicacy in parts of Europe, UK, USA etc. The microbiota of raw milk and fermented milks, cheeses, made from raw milk is complex. These microorganisms have immense role in determining quality and safety of the subsequent product made from these milks as they may be responsible in spoilage and reducing shelf life, causing human health issues, also contribute to development of flavor and aroma formation, texture development, ripening cheese. Different factors like host, stage of lactation etc. affect the composition of microbiota present in the milks and methods like cultural and culture independent methods metagenomic analysis have been done to determine the microbial community present in the milk.

Introduction

Non- cow and non-buffalo milks such as milks from goat, sheep, camel, donkey, yaks etc. are one of the important sources of nutrition in different parts of the world. In Mongolia, the highlands of the Afghanistan and Pakistan, Himalayas in India, Nepal, and Bhutan, Tibetan Plateau and in the Mountains China yak milk is the only source of milk. Cheese made from sheep and goat milk are categorized as gourmet food and many times regarded as protected designation of origin (PDO) status. Camel milk is used for medicinal food and donkey milk is suitable for children, aged people due to its hypo allergenic properties. These milks and milk products like cheese, naturally fermented milks and yoghurt are staple traditional foods in different parts of the world.

Domestication of animals was followed by the provision of shelters for protection which also modified the climate to suit the species housed for improving the productivity and reproductive efficiency. Climate is the long term average condition of the meteorological variables in a given region. The climate is divided into macro climate and micro climate. Macro climate is the general large scale climatic conditions of the open atmosphere in a large area. Micro-climate is the climatic conditions directly surrounding the animal or the physical environment in the immediate vicinity of the animal. The environment includes all the combination of conditions under which the animal lives. The immediate variables that affect the production and reproduction of animals are its micro climatic variables. The animals have requirements of their own. The environment influences where and how animal lives. Domestication has resulted in man dictating where and how animal lives. Thus breeds of livestock evolved in temperate regions have been introduced into tropical area with high rainfall, humidity and temperatures. Homeotherms are a group of animals which maintain their deep body temperature essentially constant. All the domestic animals are homeotherms. In the case of an inert body, basic thermodynamic forces establishes an equilibrium in which the body attains the same temperature of the surrounding environment. The same law applies to homeothermic animals also. Therefore, these animals will have to make compensatory physiological and behavioural adjustments to keep a constant deep-body temperature. While digestion of food takes place in the body of animals, a portion of the energy in the feed is converted into heat. This metabolic heat plus the heat gained from the environment through conduction, convection and radiation results in excess heat gain to the animal, which has to be dissipated, if heat balance is to be maintained. For this the animal depends on various physiological and behavioural functions. This balancing act by which a constant normal core temperature is maintained is known as homeothermy. Heat production is regulated by hormonal secretions, changes in muscle tone, shivering and by voluntary muscular activity. Heat loss is controlled by vasodialatation, increasing the cutaneous circulation and evaporative loss. The animal reduces its feed intake as well as production, drink more water. In long term the hair coat becomes lighter with less insulation. The animal will also exhibit postural changes and behavioural adjustments. They will try to increase surface area, reduce physical activity and seek shade.

Change in weather affects growth and development of plants. The extent of these effects depends upon the range or severity of change of weather. It is very much possible to change the weather in relatively small area to reap better harvest. Micro weather modification is defined as, “The manipulation of weather elements at increasing or decreasing the duration, intensity, quality etc., for desired effects at microlevel of crop under investigation”.

The climate near the ground can be managed partly by influencing the moisture status and temperature of the upper soil layers, e.g. by mulching and partly by action above the ground e.g. by shading or use of wind breaks.

1. INTRODUCTION

Many food components are encapsulated and stabilized by nature inside plant cells, but, with the growth in use of functional ingredients and product fortification with vitamins, antioxidants and nature-identical synthetic compounds, there is a need to stabilize and protect these ingredients from the rigours of processing and degradation during storage.

Many food products whose development was thought to be technically unfeasible are possible today because of the wide availability of encapsulated ingredients. Such ingredients are products of a process that totally envelops the ingredient in a coating or ‘capsule’ thereby conferring many useful and otherwise unusual properties to the original ingredients.

Encapsulation is a technique that is applied to preserve and/or protect numerous ingredients. In the flavour industry, the encapsulation process is used chiefly to convert liquid flavour materials to free-flowing, dried powdered products. In a broad sense, encapsulation technology includes the coating of minute particles of ingredients (e.g., acidulants, fats, and flavours) as well as whole ingredients (raisins, nuts, and confectionery products) that may be accomplished by microencapsulation and macro-coating techniques, respectively.

Micronutrient deficiency

Micronutrient deficiencies are the cause for widespread health problems, especially in developing countries. The deficiencies in vitamin A, iron, and iodine have been identified as the greatest concern, as they affect over one third of the world’s population (WHO, 1995). In addition, these micronutrients interact with each other, i.e., synergistic effects between iodine deficiency disorder (IDD) and iron deficiency anemia (IDA), or between vitamin A deficiency (VAD) and IDA to deepen their negative impacts (Lonnerdal, 2004; Lynch, 1997; Zimmermann et al., 2004). Deficiencies typically coexist in children in developing countries (Zimmermann et al., 2000).

70% of people in India do not consume enough micronutrients such as vitamins and minerals. About 70% of pre-school children suffer from anaemia caused by iron deficiency and 57% of preschool children have sub–clinical Vitamin A deficiency. Neural Tube Defects (NTDs) are the most common congenital malformation with an incidence that varies between 0.5-8/1000 births. It is estimated that 50-70% of these birth defects are preventable. One of the major causes is deficiency of folic acid. Thus, micronutrient deficiency also known as “hidden hunger”, is a serious health risk. Unfortunately, those who are economically disadvantaged do not have access to safe and nutritious food. Others either do not consume a balanced diet or lack variety in the diet because of which they do not get adequate micronutrients. Often, there is considerable loss of nutrients during the processing of food.

Introduction The Indian culinary world may have just recently embraced the concept of edible flowers, but they have been a part of traditional Indian cuisines since the beginning. Flowers have been used in human diets and gourmet preparations for thousands of years (Fernandes et al., 2020). However, due to increased consumer knowledge about the effects of nutrition on human health, there is a growing trend in utilizing flowers for edible purposes (Chatzopoulou et al., 2020). Edible flowers were used as a relish and flavour enhancer in many savoury and sweet meals in ancient Rome and Greece. In China’s ancient texts and classics, the words and phrases regarding edible blossoms were also recorded by Zhao et al. (2017). Edible flowers come in a wide variety of colors and cooking ways in different nations. Flowers, such as Osmanthus fragrans Lour and Flos Sophorae, have been employed as basic ingredients in a range of Chinese cuisines (Cunningham, 2015). The people of Rome used flowers in cooking. They make salads using violets and roses and sauces using lavenders. Flowers from pumpkin and squash plants have long been used as ingredients in the United States. People consume aromatic flower buds and unopened blossoms in El Salvador and Honduras. Blossoms are frequently used to garnish food at aristocratic banquets and feasts across Europe. Dandelion (Taraxacum officinale) flowers are also added to beverages and salads in many parts of the world.

Introduction

Encapsulation may be defined as a process to entrap one substance (active agent) within another substance (wall material). The encapsulated substance, except active agent, can be called the core, fill, active, internal or payload phase. The substance that is encapsulating is often called the coating, membrane, shell, capsule, carrier material, external phase, or matrix. In the food industry, encapsulation process can be applied for a variety of reasons. Encapsulation is a useful tool to improve delivery of bioactive molecules (e.g. antioxidants, minerals, vitamins, phytosterols, lutein, fatty acids, lycopene) and living cells  (e.g. probiotics) into foods [1]. In most cases, encapsulation refers to a technology in which the bioactive components are completely enveloped, covered and protected by a physical barrier, without any protrusion of the bioactive components. Also, encapsulation has been defined as a technology of packaging solids, liquids, or gaseous materials in small capsules that release their contents at controlled rates over prolonged periods and under specific conditions. Produced particles usually have diameters of a few nm to a few mm.

ABSTRACT

Wood apple is a hardy tree grown in arid and semiarid regions of the country. It is generally propagated through seeds. The traditional methods like budding and inarch grafting require 9 to 12 months for the root stock to come buddable size and thus 1½ to 2 years to become ready for transplanting. The present study was initiated to prepare a grafts within eight months by adopting the micrografting technique. The seeds were sown in the month of April in polybags filled with FYM, sand and soil in equal proportions with a pinch of blitox. The micrografting was done on 1½, 2, 2½ and 3 month old seedling root stock having a diameter of 0.13 to 0.15 cm, 0.17 to 0.19 cm, 0.20 to 0.22 cm and 0.23 to 0.26 cm, respectively. The seedling root stocks were decapitated with the surgical blade and 1 to 2 cm deep split was made. The scion micro bud (1 to 2 cm length and 1 to 2 mm width) of previous season’s growth was inserted in the split. The micropipette cap (0-200 μl) was inserted on the grafted portion. After sprouting of the micro bud, the micro pipette cap was removed. The study revealed that, micro bud grafting success was significantly influenced by the root stock age. Early bud take was observed when the micrografting was done on 2½ month (10.73 days) and 3-month (11.00 days) old root stock. The percent bud take on 3-month old root stock was significantly higher (99.33 per cent) than the rest of root stocks. Micrografting done on 3-month old seedling root stocks in June-July is suggested for quick multiplication of woodapple.

Purpose of Growing Microgreens in Space Farming

Microgreens are a perfect dietary ingredient in space because of their special benefits, which have been explained in earlier chapters. In an atmosphere of a spaceship with limited water and electrical power for artificial illumination, their comparatively low light and water consumption are advantageous. Furthermore, because spaceships and shelters have limited capacity, microgreens' tiny size might be advantageous. Given the right sanitization procedures, microgreens may be harvested fresh and consumed right away. Their striking colour and robust flavour may also improve meal presentation and pique astronauts' appetites. 

The science dealing with the measurement of size of any microscopically visible microorganism is known as micrometry which is based on the principle of physics. Before measuring a cell, the diameter of the microscopic field must be established by means of optic devices, namely an ocular micrometer and a stage micrometer.

a)Ocular micrometer : It is a circular disc that is placed in the eyepiece. The disc is graduated in to several small divisions marked from 0 to 100.It is placed inside the eyepiece of the microscope. The distance varies according to the objective of the microscope which can be easily determined by using a stage micrometer.

b)Stage micrometer : It is a glass slide graduated in 1 mm. Again 1mm is divided into 10 large divisions and 100 small divisions (10 x 10 = 100 divisions). These divisions are equally placed therefore 1 small division is equal to 0.01 mm or 10 μm.

The induction of mutations in polygenes controlling quantitative characters can be detected by the estimation of mean and variance of progenies from normal looking mutagen treated populations. Induction of micromutations in different crop plants have been reviewed by various investigators like Gregory (1955, 1968) in peanut, Rawlings et al. (1958) in soybean, Oka et al. (1985) in rice, Brock and Latter (1961) in Trifolium, Borojevic (1966) and Scossiroli (1966) in wheat, Gaul (1961a, 1966), Astveit (1966) in barley and Brock (1967) in Arabidopsis.

Though more reliable results can be obtained from the study of quantitative variation in the advanced generations than in M₂ (Aastveit, 1968, and Scossiroli, 1977), an estimation of the extent of induced genetic variability in quantitative traits in M₂ itself will be valuable in providing information for future selection programme. It was therefore considered worthwhile to study the shift of mean values and to gather information on induced genotypic variance heritability and genetic advance for different quantitative traits from families of M₁ from different mutagenic treatments.

12.1 Introduction

Micronization is a size reduction in which particles are reduced down to micrometer resulting in particle-size distribution is less than 10 microns. Acceleration of particles has been involved for particle-to-particle impact or impact against a solid surface. Micron technology is an emerging technology which has a great potential in nutraceuticals and functional foods. By increasing the surface area per unit mass, compared with larger-sized particles of the same chemistry, tiny particles are made more biologically active. A lot of attention has been gained by micronization in food research and development. Functional properties, surface areas and structures gets altered by reduction in particle sizes of different materials (e.g., metal salts, metal oxides, chitosan and organic compounds) by micronization or nanotechnology to micro- or nano-sizes and bring some new applications in the academic world as well as in food industry.

They absorb a large concentration (10-40 wt %) of C0₂ that either swells the polymer or melts it at a temperature below (10 to 50 °C) it’s melting/ glass transition temperature hence, this technique is useful for micronization of especially for polymers. Active substrate(s) in a polymer or other carrier substance can be used with suspensions for micronization process. In manufacturing advanced pharmaceuticals, coating materials, micro sensors, polymers and many other chemicals particle design has been gaining importance. Formulation homogeneity and control particle size can be improved by micronization. Moreover, bioavailability of poorly soluble APIs (active pharmaceutical ingredient) by increasing particle surface area and accelerating dissolution rates can also be improved. Because of the high impact velocities possible as a result of particle acceleration in a fast gas stream, fluid energy mills are used for micronization. Particle velocities in a jet mill are in the range of 300 to 500 meters per second, compared to 50 to 150 meters per second in a mechanical impact mill, in many industrial fields like food technology, pharmaceuticals, catalysts, pigments, and biopolymers, for example, are some categories of products that can be used as micro-sized particles, micronized powders are of great interest. High-temperature reactions that require high energies, on jet milling that is characterized by low efficiencies and mechanical stress, and on liquid solvents precipitation that has a poor control on particle size and can pollute the product comes under the traditional techniques for producing micronic powders. Generally, the control of the powder size and the span of its distribution are still very approximate. In recent years, many supercritical fluids-based techniques have been developed for the production of micronic and nanometric particles. Some particular properties of gases at supercritical conditions such as enhanced solubilization modulation and its power, large diffusivities, organic solvent reduced operation, and the connected possibility of controlling powder size and distribution resulted from this process. Important characteristics of powders like particle size and size distribution have significant effects on dissolution properties, flowability and release kinetics. Therefore, particle engineering is the most important step during processing of powdered foods. Lower micrometer range and sizes are needed for many applications particles with special requirements. Processes like jet milling, pearl milling or high pressure homogenization are used for micronization.