eBook Chapters

Introduction

Nano science is the study of the properties of structures of the size smaller than several hundreds of nano meter (nm). Nanotechnology consists in techniques for designing and manufacturing these structures as well as applications arising from these. The development of nanoscience and nanotechnology is in line with the trend towards miniaturisation. As nanoscience and nanotechnology developed, they make the barriers between traditional scientific and technological disciplines more permeable.

Nanotechnology is an emerging of field of science which is being highly exploited in the sphere of medicine where “nano” plays a vital role in delivery of drugs or chemical molecules to the point it is required in précised quantities in right proportions. Nanoscience infuses intelligence to the truck load of chemical constituents that are to be delivered at appropriate locations and cleave from the site after the task is complete. Such process will likely to reduce the cost besides ensuring environmental safety. Nanoscale devices with its unique properties make the agricultural system more smart and effective; such devices are capable of responding to different situations by themselves, thus taking appropriate remedial action with the need of external directions from humans. In short, these devices act as a detectors and if need arises serve as a solution/remedy for the particular issue. These smart delivery systems of chemicals in controlled and targeted manner can be considered synonymous to the proposed nano-drug delivery system in human (Patolsky et al., 2006).

Biopesticides are pesticides that are produced naturally by plants, animals, microbes, and other minerals. These represent less of a harm to humans and the environment than chemical insecticides. Nanotechnology is a rapidly developing scientific topic that has numerous uses in numerous industries, including agriculture. The transport of plant hormones, seed germination, water management, transfer of target genes, nano barcoding, nano sensors, and controlled release of agrichemicals are now being investigated as applications for nanotechnology in agriculture (Worrall et al. 2018).

Introduction

Since past decades, the application of nanotechnology in the areas of medicine, materials science and electronics have been widely accepted and appreciated due to its potential and benefits.  However, only during the recent years, researchers from other disciplines have started to see the potential applications of nanoscience in the field of agriculture (Robinson and Morrison, 2009). Recently, a generalized and process based framework to enable identification and characterization of the outputs (publications, patents etc.), and map them to the different agricultural research theme areas through the filter of links in the agri-value chain were developed by Kalpana Shastri et al., (2010) from National Academy of Agricultural Research Management (NAARM), Hyderabad, India. The frame work also permits assessing the implications for technology transfer, and impacts on society and environment. This frame work mainly relies on the identification of nano research and relating them agri-food thematic areas (Fig.1).

Introduction

The Indian poultry industry has emerged as one of the fastest growing segments in the Agriculture Sector, contributing significantly to the national GDP. The 20^th Livestock Census in 2019 estimated the total poultry population in the country at 851.81 million with an increment of about 16.8 % over the last one in 2012. The Indian poultry industry is annually growing at around 6.0% for the past four decades and is expected to touch 1066.2 million by 2030. The Per capita availability of meat and eggs is expected to increase accordingly so as to meet requirement projections made by the National Institute of Nutrition (NIN), by the year 2031. 

Introduction

By enhancing microbial populations, enzyme activity, soil respiration, and microbial biomass, nano-black carbon is a novel method for the enhancement of soil biological characteristics. By reducing soil acidity and boosting soil fertility, nano-black carbon is believed to improve plant development. The application of nano-black carbon to agricultural soils can have a significant positive impact on plant productivity. In addition to potentially boosting soil resilience to climate change, nano-black carbon greatly boosted enzyme activity, microbial activity, and soil organic carbon retention.Due to a liming action, nano-black carbon has been proposed as a potential biotechnology to boost agricultural yields in acidic soils.

Introduction

Fertilizers play an important role in improving soil fertility and productivity of crops regardless of nature of cropping sequence or environmental conditions. It has been unequivocally demonstrated that one third of crop productivity is dictated by fertilizers besides influencing use efficiencies of other agri-inputs. In the past four decades, nutrient use efficiency (NUE) of crops had hardly exceeded 35-50%, 18-20% and 30-35 % for N, P and K, respectively, despite our relentless efforts. The remaining nutrients stay in soil or enter into the aquatic environment causing eutrophication. In addition to the low nutrient efficiencies, Indian agriculture facing a problem of low organic matter, imbalanced fertilization and low fertilizer response that eventually caused crop yield stagnation (Biswas and sharma, 2008).

20.1 Introduction
  
Fertilizers play an important role in improving soil fertility and productivity of crops regardless of nature of cropping sequence or environmental conditions. It has been unequivocally demonstrated that one third of crop productivity is dictated by fertilizers besides influencing use efficiencies of other agri-inputs. In the past four decades, nutrient use efficiency (NUE) of crops had hardly exceeded 35-50%, 18-20% and 30-35 % for N, P and K, respectively, despite our relentless efforts. The remaining nutrients stay in soil or enter into the aquatic environment causing eutrophication. In addition to the low nutrient efficiencies, Indian agriculture is facing a problem of low organic matter, imbalanced fertilization and low fertilizer response that eventually caused crop yield stagnation (Biswas and sharma, 2008).The fertilizer response ratio in the irrigated areas of the country  has dcreased from 13.4 kg grain / kg nutrient applied in 1970’s to just 3.7 kg in 2005. In other words, more amounts of fertilizers is required to produce the same quantity of grain output. For instance, 27 kg NPK ha-1 was required to produce one tonne of grain in 1970 while the same level of production can be achieved by 109 kg NPK ha-1 in 2008. 

Nanofibres are an exciting new class of material used for several value added applications in the field of such as medical, filtration as barrier in composites, garments, wipes, insulation, and energy storage. Special properties of nanofibres make them suitable for a wide range of applications from medicine to consumer products and industrial to high-tech applications. The US - National Science Foundation (NSF) defines nanofibres as having at least one dimension of 100 nm or less (Hegde, 2005). Amongst the various nanostructures that have recently been developed for use in practical applications, nanofibres produced from synthetic and natural polymershave received increased attention due to their ease of fabrication and the ability to control their compositional, structural and functional properties (Burgeretal.,2006).

7.1 Introduction

Organic polymers can play important role in ecosystems by accumulating biologically important elements and also by retaining soil moisture after aggregating soil particles. Extracellular polymeric substances (EPS) play an important role in cell aggregation, cell adhesion, and biofilm formation that subsequently protect cells from a hostile environment. Furthermore, certain polysaccharides from microbial sources are surface active, and thus attempts have been made to use them as metal chelaters, emulsifiers and flocculants in industrial and environmental fields/domain. Such use of microbial polysaccharides has infused renewed interest in its production and characteristics.

Nanotechnology offers unique opportunities for the development of novel materials and applications. It is set to offer a platform to revolutionize agriculture sector from production, protection, processing and storage (Kuzma and Verhage, 2006). Bulk of nanomaterials produced is used in other sectors; only ten per cent is of use in food, beverages and packaging sector. Studies have projected the opportunities that nanotechnologies might bring to developing countries in the fields of water, energy, food and agriculture and health (Salamanca- Buentello et al., 2005) The key contributions due to application of nanotechnology in agriculture are delivery systems that aid in slow release and efficient delivery of agroinputs.

Introduction

The mankind in the past two centuries has witnessed several technology revolutions: industrial, agricultural, medical, info-tech, biotech etc. Nanotechnology is recent one in the series. Nanotechnology is the manipulation or self-assembly of individual atoms, molecules, or molecular clusters into structures to create materials and devices with new or vastly different properties. It uses techniques, processes and materials at the supramolecular level, approximately in a range between 1-100 nanometres (nm), in order to create new properties and to stimulate particular desired functionalities. For the food industry, nanotechnology applications include: release systems for pesticides or fertilizers in agriculture; antibacterial or easy-to-clean surfaces in food processing machines; food additives such as anti-caking in salt, powders and coffee creamers; anti-foaming agents for beer; natural colour additives for beverages; encapsulated nutraceuticals(antioxidants, vitamins) for dietary supplements; and, micelle systems for low-fat foods. It is predicted that in the foreseeable future nanotechnology will transform the entire food industry, changing the way food is produced, processed, packaged, transported, and consumed. The word “nano,” derived from the Greek “nano” signifying “dwarf,” is becoming increasingly too vast in terms of its huge potential in agriculture, health and the environment. In the agricultural sector, nanotech research and development is likely to facilitate next stage of development of genetically modified crops, farm and animal protection and production inputs, food processing, nutraceuticals etc. India is bestowed with a variety of crops, vegetables, fruits, flowers but food sector is handicapped due to inefficiencies in production, processing, storage and packaging. Most of the processing and packaging in industry and agriculture leaves behind a trail of environmental hazards, which if not tackled at this juncture, will add to long term ecological problems.

Over the years synthetic pesticides like herbicides, insecticides nematicides etc. have been used to improve crop yield. Indiscriminate application of pesticides ultimately leads to their excess discharge into water bodies during rainfall and causes death of fish and other aquatic life. Also consumption of seafood/fishes may cause biomagnification of chemicals in human body and leads to several lethal diseases like cancer, diabetes, neurodegenerative disorders like Parkinson, Alzheimer, and amyotrophic lateral sclerosis (ALS), birth defects and reproductive disorders. Likewise synthetic pesticides also damage soil texture, soil microbes and soil fertility. Their residues enter into plants and animal systems and further move into animals or humans body through food chain.

Abstract

Nanotechnology is an emerging field that offers various potential applications in the areas of agriculture, health and medicine. Recent advances in the design and synthesis of nano-materials aids for developing sophisticated nano-devices for clinical research and practice. Applications of nano materials in medicine includes disease diagnosis, early prognosis, detection of pathogens, tumor detection, tissue engineering, target  specific drug delivery,  development of  biomaterials  for oral health  and understanding of cellular mechanisms in live cells using molecular imaging.

Polymer Clay Nanocomposites

Polymer clay nanocomposites has been used as to affect permeability of the maternal to gases and vapours. Majority of papers on nanocomposites have been focused on the use of smectitic type clays as nanoparticles. They are a group of swelling type of clay minerals including montmorilonite, nontronite, saponite, sauconite and hectorite.

A number of polymer-layered silicates (PLS) preparation methods has been reported in literature. The three most common methods to synthesize PLS nanocomposite are intercalation of suitable monomer and subsequent in situ polymerization, intercalation of polymer from solution and polymer melt intercalation.

In general, layered silicates have layer thickness on the order of 1 nm and a very high aspect ratio (e.g. 10–1000). A few weight percent of layered silicates that are properly dispersed throughout the polymer matrix thus create much higher surface area for polymer/filler interaction as compared to conventional composites. Depending on the strength of interfacial interactions between the polymer matrix and layered silicate (modified or not), three different types of PLS nanocomposites are thermodynamically achievable (Fig. 1 and 2 ) as outlined by Sinha Ray and Okamoto (2003). They are as follows:

Introduction

Nanocomposites are materials that incorporate nanosized particles into a standard material matrix. The addition of nanoparticles can significantly improve properties such as mechanical strength, toughness, electrical or thermal conductivity. Typically, only a small amount of material (0.5-5% by weight) is required due to the effectiveness of the nanoparticles. A nanocomposite is a solid material that consists of multiple phases, where at least one of the phases has one, two, or three dimensions in the nanometer size range. The goal of the nanoscale phase process is to achieve synergy between different constituents. Nanocomposites represent a promising alternative to overcome current limitations of microcomposites and monolithics, and are becoming the materials of the future (Schmidt et al., 2002).

Introduction

Nanofertilizers are products of nanotechnology that are created or modified from conventional fertilizers, bulk fertilizer materials, or plant extracts. They use different methods such as chemical, physical, mechanical, or biological processes to produce nanosized particles that can enhance soil fertility, crop productivity, and quality (KhetiGaadi 2022). Nanofertilizers have a size range of 0.1–100 nm, which is similar to the size of plant cell wall (20 nm), making them easier to be absorbed by plants (Tiwari 2022).

Nanomaterials are a field which takes a materials science-based approach to nanotechnology. It studies materials with morphological features on the nanoscale, and especially those which have special properties stemming from their nanoscale dimensions. Nanoscale is usually defined as smaller than a one tenth of a micrometer in at least one dimension, though this term is sometimes also used for materials smaller than one micrometer.

An aspect of nanotechnology is the vastly increased ratio of surface area to volume present in many nanoscale materials which makes possible new quantum mechanical effects, for example the “quantum size effect” where the electronic properties of solids are altered with great reductions in particle size. This effect does not come into play by going from macro to micro dimensions. However, it becomes pronounced when the nanometer size range is reached. A certain number of physical properties also alter with the change from macroscopic systems. Novel mechanical properties of nanomaterials are a subject of nanomechanics research. Catalytic activities also reveal new behaviour in the interaction with biomaterials.

Nanomaterials have emerged as promising candidates for revolutionizing sensing and detection technologies, offering unprecedented capabilities in sensitivity, selectivity, and miniaturization. The current chapter provides a concise overview of recent advancements in the utilization of nanomaterials for sensing and detection applications. It encompasses a diverse range of nanomaterials, including nanoparticles, nanotubes, and nanowires, each exhibiting unique properties that enable enhanced performance in sensing applications. The integration of nanomaterials into sensing platforms has demonstrated remarkable progress in various fields, such as environmental monitoring, healthcare diagnostics & security applications.

This section explores how nanoparticles have a significant influence on sensing and detection, explaining how they have revolutionized analytical capabilities. Our trip through many kinds of nanomaterials, such as metal nanoparticles, semiconductor nanomaterials, and carbon-based structures, aims to explore their distinct features and how they might be used for novel sensing methods. The intricacies behind sophisticated sensor technologies are clarified through an examination of plasmonic sensing, quantum dot applications, and conductive nanomaterial responses. In order to improve selectivity, the chapter emphasises surface modifications and biomimetic coatings as key components of strategic functionalization techniques.

Nanomedicine, an interdisciplinary field at the intersection of nanotechnology and medicine, has emerged as a promising avenue for addressing complex health challenges. One area of significant interest is the application of nanotechnology in maintaining and improving gut health. This chapter provides a concise overview of the current state of research in nanomedicine's role in promoting gastrointestinal well-being.

Nanoparticles, with their unique physicochemical properties, offer novel approaches for targeted drug delivery, imaging, and diagnostics in the gastrointestinal tract. Engineered nanoparticles can navigate the harsh conditions of the digestive system, delivering therapeutic agents precisely to the intended sites within the gut.

Nanomedicine is the medical application of nanotechnology. The approaches to nanomedicine range from the medical use of nanomaterials, to nanoelectronic biosensors, and even possible future applications of molecular nanotechnology. Current problems for nanomedicine involve understanding the issues related to toxicity and environmental impact of nanoscale materials.

Nanomedicine research is receiving funding from the US National Institute of Health. Of note is the funding in 2005 of a five-year plan to set up four nanomedicine centers. In April 2006, the journal Nature Materials estimated that130 nanotech-based drugsanddeliverysystems were being developed worldwide.

Nanoparticle characterization is necessary to establish understanding and control of nanoparticle synthesis and applications. Characterization is done by using a variety of different techniques, mainly drawn from materials science. Common techniques are electron microscopy (TEM, SEM), atomic force microscopy (AFM), dynamic light scattering (DLS), x-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF),ultraviolet-visible spectroscopy and dual polarisation interferometry.

Whilst the theory has been known for over a century (see Robert Brown), the technologyfor Nanoparticle trackinganalysis (NTA) allows direct tracking of the Brownian motion and thismethodtherefore allows the sizing of individual nanoparticles in solution.

Nanomaterials, a cornerstone of nanotechnology, encompass a broad array of particles showcasing remarkable properties and diverse applications. This chapter outlines the classifications and applications of various nanomaterials, including organic, semiconductor, metal, metal oxide, and carbon-based nanoparticles. Each type offers unique characteristics, such as biodegradability, optical and electrical properties, making them valuable in fields like drug delivery, electronics, and catalysis. Furthermore, this study focuses on the interactions of nanoparticles with living organisms, emphasizing their impact on immune systems across plants, invertebrates, and humans. 

14.1 Introduction

The fast growing field of nanotechnology presents great potential to influence various sectors in the areas of energy, environment, agriculture, health care and consumer goods. To overcome the demanding need of nano-based applications, various strategies to synthesize nanoparticles have been attempted. The major focus is to synthesize nanoparticles of desirable properties such as shape, size and compositions. Various strategies have been employed to synthesize nanoparticles. Foremost among these are physical and chemical methods which include sol-gel technique, solvothermal synthesis, chemical reduction, laser ablation and inert gas condensation. But altogether they suffer from process limitations like difficulty in controlling experimental conditions, feeble reproducibility, polydispersity, costly instrumentation and recovery as well as energy intensive and eco-hazardous processes. However, various factors like cost, speed, efficiency, biocompatibility etc. are still not concerned. Thus, finding a balance between scalability, price and applicability is still a true challenge. These limitations can be potentially overcome by using biological methods for nanoparticle synthesis.

Agri-nanotechnology is a new branch of biology that has originated due to the compatibility of nanosized inorganic and organic particles with biological functions. Based on enhanced effectiveness, the new age drugs are nanoparticles of polymers, metals, or ceramics, which can facilitate several biological applications. Plant diseases are caused by pathogens including fungi, bacteria, mycoplasma, viruses and viroids, nematodes, parasitic plants, and protozoa. 

Introduction

Nanotechnology is a scientific and technological field that manipulates matter at the nanoscale to create, design, characterize, and use structures, systems, and devices with novel properties (Sattler 2016). Nanoparticles are one of the most important products of nanotechnology, as they exhibit different features from their bulk counterparts due to their size and shape (Sabir et al., 2014). Nanoparticles have applications in various disciplines, such as materials science, medicine, agriculture, physics, and chemistry.

Synthesis of nanoparticulate metal composites (NMCP) presents a multitude of advantages compared to conventional dosage forms. Acne, a multifactorial skin condition, predominantly involves the pilosebaceous unit and is primarily caused by bacterial species such as Propionibacterium acnes and Acne vulgaris. A staggering 95% of the global population is afflicted by acne. Leveragitng NMCP strategies holds immense promise for innovating novel, efficacious, and low-dose treatments for managing acne. This review delves into various nanotechnological methodologies tailored for effectively addressing acne. Methods: This review encompasses recent advancements pertaining to NMCP carriers aimed at treating acne.

Introduction

Nanopesticides are pesticides that use nanomaterials or nanotechnology to improve their effectiveness and safety in agriculture. Nanomaterials are materials that have at least one dimension between 1 and 200 nm. Nanomaterials have different properties than their bulk counterparts, such as higher surface area, reactivity, and mobility. Nanopesticides can be formulated in various ways, such as nanosized active ingredients, molecular active ingredient nanocarrier complexes, or active ingredients encapsulated or coated by nanomaterials (Deka et al., 2021; Xu et al., 2022).

Introduction

Plastic is the main component of environmental pollution, as it is continuously disposed of into the environment in substantial quantities. Since the creation of the first plastic polymer in the mid-twentieth century, plastic production has increased dramatically, from 1.5 million tons in 1950 to 280 million tons of plastic produced in 2011, with an annual growth rate of 8.7 % (Europe, 2017). By the 2050s, humans are expected to generate 26 billion tons of plastic waste, four times the current levels of plastic waste globally. Due to this serious concern, the United Nations has prioritised plastic waste and its management as one of the fundamental environmental concerns in its 2030 Agenda for Sustainable Development. Plastics appear in the environment in visible and invisible forms. The visible form refers to common scenes, such as bags “flying” on tree branches, PET bottles floating in rivers, lakes, and seas or face masks we use daily. The visible form of plastic in the environment is an aesthetic mockery and a reminder of the socially indiscriminate reception of all offers from the petrochemical industry. The invisible form of plastics is called nanoplastics.

The hazardous toxic metals like cadmium (Cd), arsenic (As), mercury (Hg), lead (Pb), and chromium (Cr), which have detrimental health effects, are naturally discharged into the atmosphere in various ways viz., industrial wastes, anthropogenic sources, etc. Heavy metal pollution of the atmosphere has become a frightening ecological concern that has created serious threats to the ecosystem. These metals have been identified as group I, human carcinogens due to their persistence in the surroundings and bio-magnification in biological systems. Thus, the removal of heavy metals from the atmosphere becomes a serious matter from the biological and environmental point of view. In the past following techniques adoptedfor toxic heavy metals remediation were classified are bioadsorption, ion exchange, membrane filtration, flotation, and solvent extraction techniques.

Nanotechnologies are being spoken of as the driving force behind the new industrial revolution. The mass production of these nano materials and their applications mean a dramatic increase of workers dealing with Engineered Nano Materials (ENM) and becoming possibly exposed to resulting hazardous effects and increased environmental burden of the environment due to the leaks of these materials from industrial processes. It is, hence, essential to consider the importance of ENM safety and their new applications and understand that it is important for these materials and products to be safe to enable successful promotion of nanotechnology for useful applications (Eman M Osman, 2019). 

Characterization Tools: Microscopy

Nanosensors are any biological, chemical, or sugery sensory points used to convey information about nanoparticles to the macroscopic world. Their use mainly include various medicinal purposes and as gateways to building other nanoproducts, such as computer chips that work at the nanoscaleand nanorobots. Presently, there are several ways proposed to make nanosensors, including Top-down and bottom-up design, top-downlithography, bottom-upassembly, and molecular self-assembly.

Predicted applications

Medicinal uses of nanosensors mainly revolve aroundthe potential of nanosensors to accurately identify particular cells or places in the body in need. By measuring changes in volume, concentration, displacement and velocity, gravitational,electrical, and magneticforces, pressure, or temperature of cells in a body, nanosensors may be able to distinguish between and recognize certain cells, most notably those of cancer, at the molecular level in order to deliver medicine or monitor development to specific places in the body. In addition, they may be able to detect macroscopic variations from outside the body and communicate these changes to other nanoproducts working within the body.

Introduction

Seed is the key input in agriculture deciding the fate of productivity of any crops. Indeed, seed is referred as a nature’s “nano-gift” for agriculture. Seed is a smart delivery system where we shall make manipulation to achieve the desirable goals. The seed technologists in the country were trying to find a second or third generation technologies to address the issues of seed science. Nanotechnology is one of the cutting edge research areas that has enormous potentials to solve the unresolved field problems. The application of nanotechnology to the agricultural and food industries was first addressed by a United States Department of Agriculture roadmap published in September 2000. Nanotechnology in the agricultural front has been more useful in improving the existing crop management techniques. Most of the applications of nanotechnology spearhead around nano encapsulated agrochemicals, herbicides, nano coated gene delivery and nano fertilizers (Nair et al., 2010).

In recent years application of nanotechnology in various fields including agriculture has received increased attention. Nanotechnology offers an important role in improving the existing crop management techniques especially in plant disease management by controlled and targeted release of agrochemicals to enhance their efficiency. The nano-based delivery systems are beneficial because of improvement in efficacy due to their higher surface area, higher solubility, induction of systemic activity, higher mobility and lower toxicity (Sasson et al., 2007). The pharmacokinetic parameters of nano particles may be altered according to size, shape and surface functionalization. They can also be used to alter the kinetic profiles of drug release leading to more sustained release of drug there by reducing the frequent application (Sharon et al., 2010). The successful use of metal nanoparticles in medical streams as antimicrobial agents has also led to their applications in controlling phyto pathogens (Nair et al., 2010).

Fruits and vegetables are perishables and the post-harvest losses from the point of production at the farm gate till it reaches the consumers is estimated as 30-35 per cent which constitute a major economic drain of more than 33 billion USD equivalent to Rs 2,40,000 crores annually. In order to reduce the losses at various stages, nanotechnologies have come as potential strategies to minimize the losses while improving the marketability and profitability of perishables. One of the plants derived natural compounds “hexanal” which is known to extend the shelf-life of fruits when it is used as a pre-harvest spray or post-harvest dip as a nano-emulsion. Since, it is a highly volatile compound, efforts were undertaken to entrap the compound in the polymer-based nano f ibres (as nano stickers) or as a cyclodextrin inclusion complex (as nano pellets) in order to preserve the perishables. Further, hexanal vapour was also evaluated to preserve fruits. In this book chapter, various nanotechnologies tested, evaluated and technologies developed over the years are elucidated. Overall, the literature review suggests that use of any of the nanotechnologies delivered as pre-harvest spray, post-harvest dip, nano-stickers, nano-pellets and vapour singly or in combination found to reduce the infestation of post harvest diseases, minimize the losses and extended shelf-life by 2-3 weeks that help the farmers to get lucrative prices due to the late arrival in the market besides significantly improve the productivity and profitability of orchards.

Biological diagnostic methods rely on the transmission and amplification of the pathogen in indicator hosts that develop symptoms. These methods test the biological properties of pathogens and early successes in the control of intracellular pathogens were largely due to the diagnosis of the causal agents by biological tests. The sensitivity of biological/serological tests is usually determined by the minimum number of pathogen propagules required to initiate infection. Inoculation with fewer particles could lead to false negative results. Biological techniques for disease diagnosis and pathogen detection are usually highly accurate but too slow and not amenable to large-scale application. 

Summary

Nanotechnology, the science of nanoscale structures has been applied in various fields including in biomedical sciences. Nanotechnology is being increasingly used to create materials and devices so as to interact with the body at sub-cellular scales with a high degree of specificity. Nanotechnology is used in the fight against cancer, cardiac and neurodegenerative disorders, infection and other diseases. This chapter provides an overview of some of the applications of nanotechnology in the field of biomedical sciences particularly with a focus on tissue engineering and medical diagnostics.

Nanotechnology: The Next Big Thing is Really Small

Nanotechnology can be considered as a brainchild of Richard Feynman, as the conceptual underpinings of nanotechnologies were first laid out in 1959 in his historical lecture, ‘ There is plenty of room at the bottom’. The term ‘nanotechnology’ was not used until 1974, when a researcher, Norio Taniguchi, at the University of Tokyo, Japan, used it to refer to the ability to engineer materials precisely at the nanometer level. The prefix ‘nano’ is derived from the Greek word ‘drawf’. One nanometer (nm) is equal to one billionth of a meter or about the width of six carbon atoms or ten water molecules. A human hair is approximately 80,000 nm wide and a red blood cell is approximately 7000 nm wide¹. Nanotechnology can be defined as the science and engineering involved in the design, synthesis, characterization and application of materials and devices whose smallest functional organization in at least one dimension is on the nanometer scale or one billionth of a meter^2-5. It is a multidisciplinary field, which covers a vast, and diverse array of devices derived from Engineering, Physics, Chemistry and Biology and the cumulative efforts of all the disciplines is necessary for any significant outcome.

With the development of improved systems for monitoring environmental conditions and delivering pesticides appropriately, nanotechnology can further improve our understanding of the biology of different crops and thus potentially enhance yields. It can offer routes to plant disease diagnostics, insect-pest management and efficient pesticides utilization. Nanostructure catalysts will help in increasing the efficiency of pesticides allowing lower doses to be used. 

Nanotechnology is a field of study which deals with materials at nano-scale like nanometer which is one billionth of a meter (1 nm = 10-9 meter). At nano-scale, physics, chemistry and biology converge towards the same principles and tools. Nanotechnology is domain in science and technology, which is multidisciplinary in nature and any scientist, engineer, medical doctor can do research on nanotechnology. Presently, it is more of a science and less of a technology because of a lack of proper tools for studying the properties of nano-material. Nano-particles (NPs) are materials that are small enough to fall within the nanometric range, with at least one of their dimensions being less than a hundred nanometres. This reduction in size brings about significant changes in their physical properties with respect to those observed in bulk materials. The presence of naturally occurring NPs is implicated in interplanetary and interstellar space (Hochella Jr, 2002) as well as our earth in which life from the beginning has evolved in their presence (Becker and Reitmeijer, 2006). Currently it is observed that NPs can be synthesized from many biological organisms including plants.

History and Recent Developments

Richard P. Feynman in 1959 suggested that it should be possible to build machines small enough to manufacture objects with atomic precision. His talk “There is plenty of Room at the Bottom” is widely considered to be the foreshadowing of Nanotechnology. He delivered this talk at the annual meeting of the American Physical Society at California Institute of Technology (Caltech), In 1990, a team of IBM Physicists revealed that they could write (arrange) the letter IBM using 35 individual atoms of Xenon.

In 1992 a book entitled Nanosystems: Molecular Machinery, Manufacturing and Computation was published by Eric Drexler where he outlined a way to manufacture extremely high performance machines out of molecular carbon lattice (diamondoid).

Around year 2000, federal funding for Nanotechnology in the United States began with National Nanotechnology Initiative (NNI). NNI defined nanotechnology as dealing with materials with sizes between 1 and 100 nanometers exhibiting novel properties.

The Government of India, in May 2007 launched a mission on Nano sceince and nano technology (Nano Mission) with an allocation of `1,000 crores.

Nanotechnology, which deals with understanding and control of matter at dimension of roughly 100 nm and below, has a cross sectoral application and an interdisciplinary orientation. At this scale, the physical, chemical and biological properties of materials differ from the properties of individual atoms and molecules or bulk matter, which enable novel applications. Nanotechnology research and development is directed towards understanding and creating improved materials, devices and systems that exploit these properties as they are discovered and characterized

There are many applications of nanotechnology such as in the area of medicine, chemistry and environment, energy, agriculture, information and communication, heavy industry and consumer goods. The alleged potential of this technology has garnered the attention of both developed and developing countries across the globe. The US National Science Foundation (NSF) has listed it as one of six priority areas; it is one of the themesin the EU Framework Program for Research and Technological Development in Europe; and it has been the focus of research in countries worldwide.

Introduction

Plant diseases are one of the causes of concern in agriculture and horticulture industry. Estimated global crop loss due to plant diseases exceeds hundred billion US$ worldwide (Orke et al. 1994; Narayanasamy, 2010). Plant diseases are caused by microorganism such as fungi, bacteria, viral, viroids and phytoplasma. Most of the plant pathogens are transmitted through seed or planting material, which can spread secondarily through vectors and lead to severe loss to the crop. Among plant diseases, viral diseases cause serious crop losses and affect the quality of products, while the use of virus- free seeds and planting stocks results in a substantial increase in agricultural crop production (Waterworth & Hadidi, 1998; Strange& Scott, 2005).

Indian Agriculture is facing a wide spectrum of constraints such as burgeoning population, shrinking farm land, restricted water availability, imbalanced fertilization, low soil organic carbon, besides experiencing the fatigue of green revolution and vagaries of climate change. To address all the challenges ahead, we should think of an alternate technology such as “nanotechnology” to precisely detect and deliver the correct quantity of nutrients or other inputs required by crops that promote productivity while ensuring environmental safety. The word “nano” refers to the size of one-billionth of a metre or one-millionth of a millimeter in any one of the dimension. We are aware that all materials are made up of atoms which are the smallest units and for example, ten hydrogen atoms put together will measure one nanometer. As the size gets reduced, the surface area gets increased by several folds.

Nanotechnology has the potential to revolutionizethe agricultural andfood industry with newtools forthemoleculartreatmentof diseases, rapid disease detection, enhancing the ability of plants to absorb nutrients etc. Smart sensors and smart delivery systems will help the agricultural industry combat viruses and other crop pathogens. In the near futurenanostructured catalysts will beavailable which will increase the efficiency of pesticides and herbicides, allowing lower doses to be used. Nanotechnology will also protect the environment indirectly through the use of alternative (renewable) energy supplies, and filters or catalysts to reduce pollution and clean-up existing pollutants.

Introduction

Off late our understanding of the composition and functions of the human gut microbiota has increased exponentially and there is a great interest in producing healthy foods in particular that which have an impact in improving the gastrointestinal health such as probiotics, prebiotics and synbiotics. Many of these functional foods are dairy based and have been accepted greatly worldwide. Nonertheless, there has been a need for the development of nondairy probiotic, prebiotic and synbiotic products (Salmeron 2017). This has prompted food scientists and food industrialists to study the feasibility of applying other fermenting substrates such as cereals for the development of innovative nondairy fermented functional foods. The importance of including nanoscience and nanotechnology techniques for the creation of fermented cereal beverages that contain specific bioactive nanoparticles and their role in food packaging systems will be discussed.

7.1 Introduction

Food processing has become one of the most competitive sectors in recent years. With increasing consumers’ demands, food industry needs to keep updating technology. These efforts are focussed at improving the organoleptic quality and nutrition of food and improve its keeping quality. This drives the industry to look for newer technologies which include new products as well as new processes. Some of the new processing technologies include non-thermal processes such as high pressure processing, pulsed electric fields and ultrasound processing. Advanced thermal processing techniques such as ohmic heating, inductive heating, extrusion and infrared heating have also been developed. Innovative methods for improving food quality through irradiation, removal of heat and moisture from food are now available. Newer packaging techniques now help to improve shelf-life and attractiveness of the food products and cater to the biodegradability of the packaging material. Hence, there is abundance of new products that have now become part of our daily life.

Food nanotechnology has its long history when the first step of revolution in food processing and improvement in quality of foods was introduced by Pasteur to kill the spoilage bacteria (1000 nm). Later, Watson and Crick’s model of DNA structure, which is about 2.5nm, opened the gateway of applications in biotechnology. Nanotechnology is defined as the design, production, and application of structures, devices, and systems through control of the size and shape of the material at the nanometer (10^-9 of a meter) scale. Nanotechnology is one such technology that has the potential to revolutionize the food industry. Taniguchi first used nanotechnology in 1974 to describe production technology at ultrafine dimensions. The word “nano” means “dwarf” in Greek and one nanometer is equivalent to 10^-9 m. Objects of this scale are not visible through the human eye or the ordinary optical microscope. A nanometer (nm) sized particle measures one billionth of a meter. A human hair measures 80,000 nm while a DNA strand is 2.5 nm wide whereas a protein chain is 5nm in diameter. Nanotechnology can be applied to develop nanoscale materials, controlled delivery systems, contaminant detection and to create nanodevices for molecular and cellular biology. Nanotechnology may be defined as the understanding and management of matter at dimensions of roughly 1-100 nanometers. Unique behavior of matter at the nanoscale enables novel applications. Nanoscale science, engineering, and technology can be used for imaging, measuring, modeling, and manipulating matter at this length scale. Range of sizes of nanomaterials in the food sector and their relative position on nanoscale/microscopic scale are listed below.

Nanotechnology has been successfully applied in various fields such as agriculture, biochemistry and medicine. However, its application in food systems has been new and is yet to be scrutinised. It offers a range of physical and chemical benefits that can be employed in production, development, fabrication, packaging, storage and food distribution systems. These novel nanomaterials such as nanocapsules, nanocomposites, nanoemulsions, nanoencapsulation, nano packaging, nanotubes and nanosensors have been used to improve food quality with better shelf life. However, there is concern about the safety of these nanomaterials, especially because of partial knowledge about the effects of these materials on human health.