eBook Chapters

There are two mini-cycles in the soil nitrogen cycle:

1. Soil-based processes such as mineralization, ammonification, nitrification, and immobilisation include the conversion of organic nitrogenous molecules to plant-useable mineral forms, and subsequently back to biomass (microbial, plant and indirectly animal biomass).
2. The process of converting atmospheric nitrogen into forms available to the living components of the ecosystem, i.e. nitrogen fixation and the transformations within the soil, as well as the return of the fixed nitrogen to atmospheric inert forms of nitrogen through denitrification, are all soil and biosphere resident processes.

Soil microorganisms are the most important agents in the cycling or transformation of various elements (N, P, K, S, Fe etc.) in the biosphere; where the essential elements undergo cyclic alterations between the inorganic state as free elements in nature and the combined state in living organisms. Life on earth is dependent on the cycling of nutrient elements from their elemental states to inorganic compounds to organic compounds and back into their elemental states.

The microbes through the process of biochemical reactions convert / breakdown complex organic compounds into simple inorganic compounds and finally into their constituent elements. This process is known as “Mineralization”.

Mineralization of organic carbon, nitrogen, phosphorus, sulphur, and iron by soil microorganisms makes these elements available for reuse by plants. In the following paragraphs the cycling or transformations of some of the important elements are discussed.

To meet the food needs of the burgeoning population, India will need to produce 300 million tonnes of food grains by 2020. At present more than 75% of the total food grains produced in the country are of rice and wheat. Use of nitrogenous fertilizers has contributed much to the remarkable increase in production of rice and wheat in India that has occurred during the past three decades. During the last half-decade or so while fertilizer N consumption is touching new heights, the production of both rice and wheat is showing a trend of plateauing. In fact, fertilizer N efficiency of food grain production expressed as partial factor productivity of N (PFPN) has been decreasing exponentially since 1965. The PFPN is an aggregate efficiency index that includes contributions to crop yield derived from uptake of indigenous soil N. fertilizer N uptake efficiency, and the efficiency with which N acquired by the plant is converted to grain yield. A decrease in PFPN occurs as farmers move yields higher along a fixed N response function, unless other factors shift the response function up. In other words, an initial decline in PFPN is an expected consequence of the adoption of N fertilizers by farmers and not necessarily bad within a system’s context

Under agricultural conditions, the nitrogen removed is usually greater than the nitrogen input. To maintain fertility, nitrogen must be returned to the soil. This takes place mainly by biological nitrogen fixation, which is responsible for the major reduction of dinitrogen to ammonia that is used by the plants. Biological nitrogen fixation accounts for most of the fixation of atmospheric nitrogen in to Ammonium, thus representing the key entry point of molecular nitrogen into the biogeochemical cycle of nitrogen (Fig.4.1). Some bacteria can convert atmospheric nitrogen into ammonium. Most of this nitrogen fixing prokaryote is free living in the soil.

Nitrogen is the most important element on which depends growth and productivity of plants. It is also the most abundant molecule in the atmosphere (80%), yet it cannot be utilized by plants. The plant utilizable form of nitrogen is either, its most oxidized form, the nitrates or the most reduced state, the ammonia.  The indiscriminate use of nitrogenous fertilizers amounts to depletion of non-renewable fossil fuels used in fertilizer production. Sustainable agriculture depends largely on renewable resources and on-farm nitrogen contributions are achieved largely through biological nitrogen fixation (BNF), which helps in maintaining and/or improving soil fertility by using atmospheric nitrogen. Annually BNF is estimated to be around 175 million tonnes of which close to 79% is accounted for by terrestrial fixation.

Although the Dacron bag technique for protein degradation measurement is relatively simple and not too expensive as compared to in vivo measurements, the technique is not easily adaptable in laboratories that are not fully equipped to maintain cannulated animals. Therefore, different alternative approaches such as measurement of protein solubilities in buffer, enzymes and detergent solutions have been tried (Pichard and Van Soest, 1978; Krishnamoorthy et al., 1982, 1983, 1995; Aufrere et al.,1991; Prabhu et al., 1996; Licitra et al., 1996 and 1998). The advantages of these approaches are that, they are simple, rapid, economical and repeatable. However, since the enzymes used are not of rumen microbial origin, degradation obtained from these enzymes may not be a true .reflection of degradation occurring in the rumen. Nevertheless, because of their simplicity and rapidity, in situations where large number of samples are to be compared, in vitro techniques serve as useful tools.

Introduction

As per statement of Dr. Henneberg of Weende Experiment Station (Göttingen, Germany) the carbohydrates are divided into two groups; crude fiber and nitrogen free extract. The nitrogen free extract, which  comprises the sugars, starch and a large part of the material classed as hemicellulose, is determined by difference. It is represented by the figure obtained when the sum of the water, ash, protein, fat and crude fiber of a feed is subtracted from 100. Since the figure is determined by difference instead of directly, it includes the cumulative errors of the other determinations and thus is not an exact value.

21.1 Determination of Total Nitrogen in Soil (Modified Kjeldahl Method)

Nitrogen mostly present in soil in organic form. Relatively small amount of nitrogen usually occurs in ammonium and nitrate form, the available form. The Kjeldahl method of total N determination includes both organic and ammonium forms and with modification nitrate form of N can also be included.

Principle

When soil is digested with concentrated sulphuric acid organic nitrogen is converted to ammonium sulphate. To speed up the reaction a digestion mixture composed of sodium (or potassium) sulphate (to raise the boiling point of H₂SO₄) and cupric sulphate (CuSO₄) - selenium powder (catalyst) is used.

14.1. Determination of Total Nitrogen in Soil (Modified Kjeldahl Method)

Nitrogen mostly present in soil in organic form. Relatively small amount of nitrogen usually occur in ammonium and nitrate form, the available form. The Kjeldahl method of total N determination includes both organic and ammonium form and with modification nitrate form of N can also be included.

Abstract

Nitrogen is the most limiting nutrient controlling production of all agricultural systems. Application of N fertilizer has increased food production tremendously all over the world. However, it also contributes towards global warming and ozone layer depletion through emission of nitrous oxide, a greenhouse gas (GHG). Therefore, there is a need to assess the possibilities of N management for climate change abatement and food security enhancement, while minimizing environmental and human health impacts. In this chapter, role of gaseous-N emission on N use and various mitigation options for Indian agriculture have been discussed. For the base year 2007, the direct and indirect N₂O emissions from Indian agricultural soils was estimated to be 136.29 Gg (35.3 Tg CO₂ equivalent) and 30.61 Gg (5.8 Tg CO₂ equivalent), respectively.

Before the invention of N fertilizer, mineralization of organic N ( present in dead plants and animals or manure) was the main method used to obtain ammonium and nitrate nitrogen. Mineralization is conducted by soil microbes resulting in ammonium ion. Most Nitrogen is absorbed by plants in the form of Nitrate (NO-₃). Ammonium is converted to Nitrate with the help of  soil bacteria. Nitrogen is a very mobile element and is easily lost from the soil. Ammonium (NH₄+) can be converted to Ammonia (NH₃). The gaseous ammonia can be lost to the atmosphere.  Nitrate can be lost through denitrification. Nitrate nitrogen is reduced to nitrous oxide (N₂O), elemental nitrogen (N₂) and nitric oxide (NO). All are volatile. Denitrification occurs under conditions of high water availability and temperatures higher than 10^ºC.

Abstract

The abiotic stresses viz. drought, high temperature, water stress, salinity, heavy metal contaminations etc. cause variable effect on nitrogen (N) mineralization, its uptake and transport, assimilation and remobilization, and subsequently the growth and productivity of plants. The affect is generally negative, however, the magnitude of decrease in various processes is dependent on genotype, age of the plant, tissue under investigation, type and extent of the stress and other agro-climatic conditions. Biological nitrogen fixation and nitrate assimilation appear to be more sensitive than the ammonium assimilation possibly because re-assimilation of ammonia released in the tissue due to catabolism of N molecules is essential for the survival of plants. In addition, synthesis of stress metabolites (proline, glycine betaine and poly amines) is directly dependent on the availability of primary amino acid, glutamic acid. There are few well coordinated and detailed studies, determining the key factors involved in regulating NUE under abiotic stresses.

Abstract

Nitrogen is an important nutrient for growth and development of plants. Presence or absence of nitrogen often affects the amino acid composition of proteins, which may in turn change its nutritional quality. Increase in the elevated CO₂ concentration and temperature associated with future climates are expected to affect the nitrogen use efficiency (NUE), growth and grain yield of wheat (Triticum aestivum L.). Atmospheric CO₂ is steadily rising from the current level of approx 384 µLL^-1 and is expected to rises upto 550 µLL^-1 by 2050. It is predicted that the global temperature would increase by 1.5- 4.5^oC. The main future challenge is to feed nine billion people under an altered climate, as this increase in global temperature would affect, negatively or positively the future crop productivity including wheat depending upon the geographical location. The present book chapter summarizes the potential impact of elevated CO₂ and temperature on NUE of Triticum aestivum L.

Greek scientist Theophrastus (300 BC) advocated legume rotation. As early as 1750 AD it was known that plants require Nitre. Louis Pasteure in 1860 AD stated the possibility of atmospheric nitrogen fixation by microorganisms. Hellriegel and Willfarth (1886) strengthened the views of Louis Pasteure by his observations on the irratic behaviour of legumes to nitrate nutrition. Beizerink (1890) isolated the organism involved in nitrogen fixation. The systematic field experiments conducted at Rothemstead experimental station showed that the plants respond to inorganic nitrogen.

ROLES OF NITROGEN IN CROPS

Plants contain 1-5 per cent nitrogen by weight. It is a structural component of chlorophyll, aminoacids and proteins. Plant proteins contain 16 per cent nitrogen (6.25 × %N = protein). Plants are dependent on protein for their propagation. Cereals possess a threshold protein level below which grain will not form.

Introduction

The enzyme nitrogenase is produced in all N₂-fixing systems. The activity of nitrogenase enzyme relates to the biological nitrogen fixation, which can be determined directly by measurement of fixation product, ammonia and the further products of its assimilation. The activity can also be measured indirectly by measuring the ability of nitrogenase to reduce the number of substrates other than N₂. Substrates such as nitrous oxide (N₂O), azide ion (N₃^- ), cyanide (CN^-) and isocyanide (NC^-) are reduced and the requirements for ATP and reductant are similar to those of N₂ fixation. The reduction of protons to molecular hydrogen has been used to measure the nitrogenase activity.

Dilworth (1966) and Schollhorn and Burris (1966&1967) observed the inhibitory effect of Acetylene (C₂H₂) on N₂ fixation by extracts of Clostridium pasteurianum. Dilworth (1966) further observed by mass spectrometry that the C₂H₂ was reduced to C₂H₄. Both gases are readily measured at very low concentrations by gas chromatographic techniques.

Nitrogen (N) is an essential element for all forms of life because it is a major component of amino acids, which are the building blocks of proteins, nucleic acids such as deoxyribonucleic acid (DNA) and other cellular constituents. It is a structural component of chlorophyll in plants, which is used in photosynthesis to make their food. Nitrogen ranks first among the essential nutrient elements required for plants. Its careful management is very important for crop production.

Nitrogen exists as two main stable isotopes (isotopes of a given element have the same number of protons but different numbers of neutrons in each atom) namely, ¹⁴₇N (abundance 99.63%) and ¹⁵₇N (abundance 0.37%). These can be separated by chemical exchange or by thermal diffusion. Both ¹⁴₇N and ¹⁵₇N isotopes of N are stable isotope which do not disintegrate as in case of other radioactive isotope like ³² ₁₅P. There are four other isotopes of N viz. ¹²₇N, ¹³₇N, ¹⁶₇N and ¹⁷₇N, mostly are radioactive having very short half-life and not used in agriculture. The ¹³₇N has a half-life of about 10 min, while other three have half-life of only few sec. The ¹⁵₇N (tracer) isotope is used as a tool for determination of pathways of transformation of N in soil or plant or animal.

The major part of N comes from atmosphere (air). On the basis of volume by volume (v/v), air contains about 78-79% N₂, 20-21% oxygen (O₂) and 0.03% carbon dioxide (CO₂). Most living organisms cannot utilize N₂ gas present in the atmosphere directly because it is present as di-nitrogen form or di-atomic form (N≡N). It is called as an inert gas. Therefore, to break this triple bond, huge amount of energy is required. After breaking of triple bond of N₂ molecules, it combines with other element particularly with O₂ or hydrogen (H) to form oxides of N (NO, NO₂, NO₃ and N₂O) or ammonia (NH₃). Thus, breaking of triple bond is must for forming NH₃ which further can be used as the key material for almost all fertilizers. Therefore, the fixation of N₂ is vital for production of all nitrogenous and complex fertilizers like NP/NPK and nitrophosphates.

Nuclear Magnetic Resonance spectroscopy is a powerful and theoretically complex analytical tool. The nuclei of certain isotopes are continuously spinning with an angular momentum, which can give rise to an associated magnetic field.

Edmond Nocard (French Veterinarian) first isolated filamentous aerobic bacteria from clinical cases of ‘bovine farcy’ in 1888. In 1889, the genus Nocardia was formed by Trevisan, and the organism described by Nocard was named as Nocardia farcinica. In 1891 another similar organism (Nocardia asteroides) was isolated by Eppinger from a human brain abscess. Initially it was thought that they are the same organisms. Latter on the basis of the molecular studies their separate entity is established.

            However, 80 years later, re-examination of Nocard’s original isolate revealed two different filamentous organisms, one of which was Mycobacterium and the other was Nocardia. The cause of ‘bovine farcy’ has been subsequently attributed to Mycobacterium. Latter, it is proved that Mycobacterium farcinogenes is the actual causative agent of ‘bovine farcy’.

Noise has become a very important “stress factor” in the environment of man especially with regard to health. However, not much attention is being paid towards its elimination or control in dairy plants. The harmful effects of noise(s) in the workplace as an occupational hazard is really a cause of great concern. Apart from reducing the work efficiency, in the long run it causes serious auditory and non-auditory effects on the worker. Thus, it becomes imperative to set legal standards in workplaces to be monitored constantly to avoid or reduce the health hazards of the workers.

Noise and Noise Pollution

Noise is often defined as “Unwanted Sound” or “Undesirable Sound”. The “Noise Pollution” has been coined to signify the vast cacophony of sounds that are being produced in the modern life, leading to health hazards.

For the hundreds of thousands of years that we lived on earth,there were few rapid changes in the atmosphere. It is only in recent times, particularly since the Industrial Revolution, that our activities on earth began to modify our environment in a significant manner.  Industrial pollution has affected the water we drink, the air we breathe, and in general,the biosphere itself.Now noise has joined the bandwagon of pollutants.

Noise certainly comes within the scope of definition of a pollutant that “..... any undesirable change in the physical, chemical or biological characteristics of our air, land and water that may or will harmfully affect human life or that of any other desirable species or industrial process, living conditions or cultural assets, or that may or will waste or deteriorate our raw material resources.”

A sound is a form or energy. Therefore, it has to have an impact on life in one way or the other. Most of the organisms of Kingdom Animalia communicate with each other creating a peculiar sound. Creating sound is inevitable for performing various life activities. Every animal species has the characteristic sound it reflects through its behavior. Whatever all the animals communicate through creating specific sounds is all-natural. Nature, as such is a stupendous repository of a diversity of sounds, all being specific characteristics of life.

Human behavior, however, is highly evolved in terms of creating a variety of sounds. Communication, entertainment, and many behavioral activities are expressed through creating distinctive sounds. There is a difference between natural sounds and the ones human beings create or can create. Humans often generate sounds, not just in a natural manner, but also with various technical aids, that often become unpleasant what we call noise. Creating noise, or noise pollution, seems to be an exclusive characteristic of the human species. Of course, there could be some natural sounds that might occasionally cause havoc to life. Examples are the sounds generated at the time of the volcanic eruption, during an earthquake

Taxonomy

Taxonomy is the science that deals with the identification, nomenclature and classification of organisms.

Systematics

It is scientific study of organisms with the ultimate object of characterizing and arranging them in an orderly manner.

Classification

Classification is the ordering of organisms into groups and systems, based on their morphological, phylogenetic and physiological relationships.

Nomenclature

It is the system of assigning names to the taxonomic groups or organism according to international rules.

Principle
(Method of Patterson 1963)

The albumin bound long-chain fatty acids of plasma are usually referred to as unesterified or non-esterified fatty acids (NEFA) to distinguish them from the long-chain fatty acids present in the esterified form in neutral fats and phospholipid. In the case of ruminant plasma, it is also important to distinguish these “free fatty acids” from the small molecular weight steam volatile fatty acids of dietary origin which are found in the free state as the carboxylate ion.

Basically, the method of Dole (1959a) has been used to estimate plasma NEFA employed by Annison (1960), which is to evaporate the heptane extract of plasma to dryness before titration. This is conveniently done in a stream of nitrogen on a warm hot plate rather than by evaporation in vacua. In place of the 0.05 M- phosphate buffer (pH 6.0) of Annison or the 0.02 N-H₂SO₄ used by Dole (1956a), in the present method 0.02 N-HCI is used as an extraction medium. This is preferred because owing to its volatility, it is less likely to cause serious accidental contamination of the supernatant heptane layer.

26.1 Introduction

The egg’s primary non-food purpose is to help the species reproduce. The quantity of eggs required for the hatching of chicks has increased significantly due to the substantial rise in meat consumption. The USDA has made a list of a few other non-food use for eggs.

Inedible eggs are often used as animal feed and plant fertilizers. Eggs are also fed to show animals, such as dogs and horses, to improve the glossy sheen of the fur coat. Industrial egg albumen is used in finishing several types of leather, notably coloured stock, and glazed. production of vaccines in chick embryos is one of the key uses of eggs, as per various researchers.

In the past several Non infectious diseases (NIDs) of fish and shellfish have been observed in natural waters. However, with expansion in fresh, brackish and marine aquaculture including aquarium keeping world wide there is a phenomenonal increase in incidences of NIDs and economic loss. Common Non infectious diseases (NIDs) in fish are 1.Gas bubble disease(GBD), 2. Body deformities(BD), 3.Nutritional diseases(ND) and 4. Disease due to harmful algal bloom(DHAB)

1. Gas Bubble Disease (GBD)

Gas bubble disease (GBD), is caused by supersaturation of gases in water. The gas involved is nitrogen in most cases and rarelycarbon dioxide and oxygen can cause GBD as these two gases are used by fish tissues and readily processed.Oxygen can cause gas-bubble disease at about 350 per cent air saturation, but nitrogen can cause the disease even below 118 per cent. GBD becomes prominent whenever there is a change in temperature, pressure and turbulance in aquatic environment, leading to disturbance in metabolism of f ishes. Bubbles of gas may form in the eyes, skin, gills, fins and within the internal organs where small bubbles may coalesce into larger bubbles.Fish absorb the excess gas, which forms bubbles in the small blood vessels, due to pressure changes inside their body. Gas bubble disease (GBD), is seen both in captive and wild fish. When bubbles affect internal organs, some fish may diesometimes without obvious clear clinical signswhile other fish may be chronically affected, and have a reduced appetite, activity and buoyancy. GBD can also cause secondary bacterial, viral or protozoan infections.All fish and shelfishspecies as well as amphibians and aquatic invertebrates are susceptible to GBD.Sensitivity of fish to GBD in terms of pathology and mortality varies with species and age.

11.1. BORON TOXICITY
11.1.1. Introduction

Boron (B) is essential micro-nutrient. It is necessary for cell wall formation, membrane integrity, calcium uptake and carbohydrate synthesis and transportation. B affects many functions in plants, which includes flowering, pollen germination, fruiting, cellular activities (division, differentiation, maturation, respiration etc.), water relationships and the movement of hormones. It is not translocated and leached easily from soils.

Nitrate – Nitrite Poisoning
Sources

Accidental ingestion/grazing of
Plants which are Nitrate accumulators

True/False

Q.1.    The most fundamental assumption for “Parametric inference” is that the random sample drawn is from “Normal” population.
Q.2.    The most fundamental assumption of “Non-parametric” test is that the random sample drawn is from “Continuous distribution”
Q.3.    The standard parametric methods are applicable for “Estimating the parameter” as well as for “Testing the hypothesis” about the parameters.
Q.4.    Non-parametric methods, are applicable only for testing the hypothesis about  distribution but not applicable for estimation purpose of parameter.

Q.1 The most fundamental assumption to be followed by random variable for using the standard method (parametric statistic) or parametric inference is that the random variable follows normal distribution.

Q.2 The standard parametric techniques are not useful for estimating parameters and testing hypothesis about them.

1.    UV irradiation is considered better than other irradiation methods in terms of

(a) Physical structure maintenance

(b) Chemical structure

(c) Sensory quality

(d) Microbial quality

2.    Irradiation with UV light is considered as

(a) Sterilization method

(b) Disinfection method

(c) Pasteurization method

(d) Cooking method

3.    UV treatment kills bacteria, viruses, fungi due to its effect on

(a) Metabolic pathway

(b) DNA

(c) Signaling pathway

(d) ATP generation

4.    Effectiveness of UV radiation on micro organisms depends on

(a) Species and strain

(b) Spore formation

(c) Culture and growth

(d) Both (a) and (c)

Abstract

Ghee means the pure clarified fat derived solely from milk or curd or from desi (cooking) butter or from cream to which no coloring matter or preservative has been added. It is the second largest dairy product prepared from milk and it is almost 35% of total milk production. Unfortunately, the producers or the middle- men involved in the ghee trade, tend to adulterate ghee with cheaper oils and fats like vegetable oils, animal body fats, hydrogenated fats and sometimes even the non-edible mineral oils, especially during lean season to earn more money. Recently, the problem of adulteration has assumed a very serious dimension. Such a situation has tarnished the image of dairy industry. Methods presently adopted by food law enforcing agencies to ensure the quality of ghee are mainly based on the physico- chemical constants, but all these methods have some limitations. So researchers are always in search of different innovative methods to check the quality of ghee.

Definition and meaning – Proxemics, Chronemics, Movement and body position, Posture, Facial Expression, Gestures and Eye Contact – importance of non verbal communication

Meaning

A message can be sometimes expressed without the help of words. Nonverbal communication is the process of communicating without the use of words.

Definition

It is defined as non-word human responses like facial expressions and gestures and the perceived characteristics of the environment through which the human verbal and nonverbal messages are transmitted. Nonverbal communication is also known as “silent language.” It involves the use of cues, gestures, vocal characteristics, facial expressions, and spatial relationship between the sender and the receiver to convey a message. For example, a smile, glance, stare or a frown convey different meanings.

Food technologies now have more sweeteners from which to choose than ever before. With the obesity epidemic and the public attention it currently receives, plus the increasing interest in foods with added benefits sweeteners that allow for consumer appealing labeling are of particular interest. These sweeteners fall into basic categories, those that are essentially calories free, often referred to as low-calories or intense sweeteners, and those, which are significantly reduced in calories, which may be referred to as reduced calories-sweeteners, bulk sweeteners, or sugar replaces. These sweeteners when used alone or in combination may permit such labeling as ‘low-calories’, ‘reduced-calories’, ‘light’ ‘sugar-free’ and ‘dose not promote tooth decay’.

The ideal sweeteners does not exist, it would be at least as sweet as sucrose and provide the same properties to a product as sucrose, with processing parameters similar to those of sucrose so that existing equipment can be used. It would be colourless, odourless and noncariogenic, with a clean, pleasant taste and have immediate onset and not much lingering. Solubility and stability are important. The ideal sweetener must be compatible with a wide range of food ingredients because sweetness is but one element of a complex food flavour system. Even sucrose is not perfect, being unsuitable for some applications.

Introduction

Soil is a vital, complex, and firm layer of the land surface that provides a source of minerals and organic matter interacting with air and water to harbor life forms. (Lobmann et al., 2022). Versatile micro and macro living organisms, i.e., bacteria, fungi, and actinomycetes, inhabit soil and contribute to its quality, health, and inevitable role in serving the environment (Elsakhawy et al., 2022). Soil roots for a variety of crucial functions which involve ecosystem services or socioeconomic activities, e.g., biomass production, climate gas fluxes of CO2 , N2 O, and CH4 , water cleaning, storage and supply, foundation of construction and provision of construction materials, or provision of habitats etc. The responsibility of soil in sustaining life on earth by providing and conserving resources is not negotiable.

Abstract

Probiosis has emerged in the early 20^th century after Ellie Metchnikoff proposed his hypothesis that organisms were reponsible for longivity of ethnic people of Balkan region of Russia and ever since it has grow into a major scientific field which has its applications extending into farming, aquaculture and prophylactics in humans. Vast research was carried out on this topic and Lactobacillus sp. and Bifidobacterium sp, are the two important genus that are highly studied and widely recognized universally as conventional probiotics. Apart from these two genus there exists many other bacterial species which have shown promising probiotic potential. Such non conventional probiotics are widely used in some parts of world as commercial food products with health beneficial effects, pharmaceutical products or being tested for their safe utilization in human beings. The present chapter tries to covers some genera and their potential and application in prophylactics.

1. Introduction

Vegetables and fruits are the next in demand for human consumption after rice and wheat, in all parts of the globe. Fresh fruits & vegetables and their value added products are increasing in popularity day by day almost in all the countries, because of their richness in dietary fibre, minerals & vitamin contents and for their medicinal properties. Maintaining agricultural produce quality and its evaluation are thus naturally important. Decisions concerning maturity and ripeness of fruits and vegetables are based mostly on visual inspections, which are subjective in nature. The quality of fruits and vegetables is mostly decided on the basis of size, shape, colour, gloss, flavour, firmness, texture, taste and freedom from external as well as internal defects. Internal quality factors such as maturity, sugar content, acidity, oil content and internal defects, however, are difficult to evaluate non-destructively. Methods are needed to better predict the internal quality of fruits and vegetables without their destruction. Recently, there has been an increasing interest in non-destructive methods of quality evaluation, and a considerable amount of effort is being made in this direction. But the real problem is how these methods are to be exploited practically on commercial scales and what are the constraints faced in implementing them.

1. INTRODUCTION

Fruits and vegetables are increasing in popularity in the daily diet of people of both the developed and developing countries. Product quality and quality evaluation methods are extremely important. The decisions concerning harvesting, maturity, ripeness and quality are based mostly on subjective and visual inspection of the external appearance of the fruits. The quality of fruits and vegetable is mostly based on size, shape, colour, gloss, flavour, firmness, texture, taste and freedom from external and internal defect. Many techniques for evaluating the external quality parameters are now available commercially. Internal quality parameters such as sugar content, acidity, oil content and internal defects however, are difficult to evaluate. Methods are needed to predict the internal quality of fruits and vegetable better without destroying them. Recently, there have been an increasing interest in non-destructive method of quality evaluation and a considerable amount of efforts has been made in this direction. The development of science and technology, especially of information technology has made available many non-destructive methods of analysis of materials which can also be applied to fruits and vegetables. The fruit industry needs non-destructive techniques for online sorting and certifying high quality fruit.

Non-destructive method can be classified according to the principle used to detect fruit properties; mechanical, acoustic, optical and Nuclear Magnetic Resonance (MRI) Techniques.

Any kind of developmental programme needs a proper integration of different parts of the society i.e. stakeholders of that programme. Apart from the nodal body, many implementing agencies take part in the implementation, monitoring of those programmes. Non-Government Organizations are one of those who actively participated in developmental activities not only as an implementing agency but also in mobilizing the resources, proper utilizing that and also effectively utilizing the resource towards development. NonGovernment Organization is an organization that is independent in nature, politically, socially unbiased and has a proper legal stand with developmental orientation.

Introduction

Of the five human sensory perceptions, the feelings of sight, hearing and touch have been simulated by artificial sensors quite successfully. Electronic eyes (digital cameras), electronic ears (microphones) and touch sensitive electronic devices are quite common nowadays. In comparison to these devices, the perception of smell and taste are relatively unexplored. Sensing of smell and taste is full of uncertainty and the prevalent technology is at its infancy. Electronic noses for smell and electronic tongues for taste or a combination of both have vast prospects in the field of standardization of quality of food and agro products. In India, proper use of these instruments can revolutionize the quality of the food and agro products, thereby increase the export worthiness of these products. In this article, we have tried to discuss applicability various electronic olfaction technologies like electronic nose and electronic tongue for fish freshness determination. A report on an experiment with electronic nose on fish samples has been presented.

Nitrate - Nitrite Poisoning
Sources
Accidental ingestion/grazing of Plants which are Nitrate accumulators 

Introduction

“Non-monetary inputs are defined as those cultural operations which help to achieve high yield at no extra cost and whose cost does not change with the level of output”.
 
After the advent of green revolution, nutrient management has received the significant attention by farmer as well as researchers; as a result of which and coupled with the requirement for high yielding varieties, consumption of plant nutrient (N, P and K) increased tremendously. Variability was observed in fertilizer consumption between the states. 

Parametric and Non-parametric tests

Parametric and Non-parametric tests are used for comparing differences between groups (e.g. Difference in catch-per-unit-effort (CPUE) of two types of Codends) and to test the relationship between two or more variables (e.g. Gender differences in awareness levels of fish farming (High, medium and low). As there seen differences in types of data, Non-parametric statistics are used when

• Data is non-normal

• Small sample size

• The variables are categorical

• Short response categories (<5 ) in scales

15. 1 Introduction

Data collected in social sciences and economic study, are usually based on answers provided by the respondents. These answers are aften based on re-collection of past memories and often suffer from memory lapses, intentional erros etc. As such they provide only a crude informations which are never exact. The analysis of such data on the pattern of other biological data will not yield much information because many times they will not fulfil the underlying assumptions. For such data, the non-parametric (N.P.) methods are most appropriate.

15.2 Parametric Tests

The tests which deal with the parameters of the populations, are known as parametric test e.g. Z, t & F tests are the parametric tests.

A non parametric test (sometimes called a distribution free test) is a statistical analysis method that does not assume the population data belongs to some prescribed distribution which is determined by some parameters. That means, a non-parametric test can be defined as a test that is used in statistical analysis when the data under consideration does not belong to a parameterized family of distributions. When the data does not meet the requirements to perform a parametric test, a non-parametric test is used to analyze it. The non-parametric test compared to parametric test, which makes assumptions about a population’s parameters (for example, the mean or standard deviation); When the word “non parametric” is used in stats, it means that population data does not have a normal distribution.

If at all possible, we should us parametric tests, as they tend to be more accurate. Parametric tests have greater statistical power, which means they are likely to find a true significant effect. Nonparametric tests can perform well with non-normal continuous data if the data of the sample are sufficiently large. Non parametric do not assume that the data is normally distributed. The only non parametric test we are likely to come across in elementary stats is the Chi-square test. In nonparametric tests have several advantages as compared to parametric tests. It includes:

• More statistical power when assumptions for the parametric tests have been violated.

• Fewer assumptions, i.e. the assumption of normality don’t apply.

• Small sample sizes are acceptable.

• It is used for all data types, including nominal data, rank data or ordinal data

• Knowledge of the population is not required to conduct this test.

• Nonparametric tests are valid when our sample size is small and our data are potentially non-normal.

1. Ammonia Nitrogen by Distillation Method
Method I (For Animal Feeds)
Reagents
    1.    Mangnesium oxide (carbonate free)
    2.    Standard sodium hydroxide 0.25 N
    3.    Standard sulphuric acid 0.5 N
    4.    Methyl red indicator

Weigh accurately 2 to 4 g  of the prepared sample. Shake it with water and filter. Wash the residue thoroughly with water. Transfer the filtrate to the distillation flask and dilute to about 200 ml with water. Add about 5 g of the magnesium oxide, Connect the flask to the condenser by means of the connecting bulb tube and distill about 100 ml of liquid into the receiver containing standard sulphuric acid and methyl red indicator solution, Titrate the contents of the receiver with the standard sodium hydroxide solution. Carry out a blank determination using all reagents in the same quantities but without the material to be tested.

INTRODUCTION

Food processing as a technological regime is undergoing a revolutionary change in terms of conception, product and process design and energetics. The conventional approach of a food processor is to develop products with a long shelf-life in user friendly packaging systems. Food processors are usually bothered about sensory attributes and microbiological profiles without much emphasis on the fate of heat labile nutritional and nutraceutical components. The changing consumer perception is slowly but steadily leading towards emergence of novel technologies without depending upon conventional thermal processing. A few years before, non-thermal techniques have emerged for food processing and preservation, but these techniques were restricted to framing of fundamentals with regard to the inactivation of vegetative and reproductive bodies of microbes and the deteriorative enzymes.

Food processing involves the transformation of raw materials into consumer-ready products, with the objective of stabilizing them to prevent negative changes in quality and ensure food safety. To consumers, the most important attributes of a food product are its sensory characteristics like texture, flavour, aroma, shape and colour. The aim of food manufacturers is to develop and employ processing technologies that retain or create desirable sensory qualities or reduce undesirable changes which take place during processing. Alternative or novel food processing technologies are being explored and implemented to provide safe, fresher-tasting, nutritive foods without the use of heat or chemical preservatives. The major non thermal technologies gaining importance are High-pressure processing, Pulsed light technology, Pulsed electric field, Irradiation etc.

High pressure processing

The application of very high pressures (upto 87,000 psi, 6000 bar or 600 MPa) for preservation of food substances in combination with or without heat is known as high pressure processing (HPP). This process is also known as high hydrostatic pressure processing (HHP) or ultra-high pressure processing (UHP). When compared to thermal processing, pressure treated foods have a fresher taste, better appearance, texture and nutritional value. High pressure processing can be conducted at ambient or refrigerated temperatures, thereby eliminating thermal effects and cooked off-flavors. The technology is highly beneficial for heat sensitive products. The first high pressure processing line was introduced in Japan for jam manufacture in 1990’s and has since been upgraded to several food products. A number of HPP products have been commercialized in North America, Europe and in China. Machines are now available with operating pressures in the range 400-700 MPa and capacities ranging up to 900 kg per batch. Since HP processing affect mainly the non-covalent bonds of the food, the quality characteristics of foods such as color, flavor and nutrients generally remain unaffected (Knorr, 1993).