eChapters

The respiratory system is a complex biological system comprised of several organs that facilitate the inhalation of oxygen and exhalation of carbon dioxide (breathing) in living organisms. The major functions of the respiratory system is providing oxygen to the body cells and removing carbon dioxide from the body. In addition to this, the respiratory system helps in controlling body temperature (especially in dogs), eliminating water (as vapour) and assisting in voice production. The organs of respiratory system extend from nose to lungs. These consist of two major parts, the upper respiratory tract and the lower respiratory tract. The upper respiratory tract is comprised of nose, nasal cavity, the part of mouth, and the part of pharynx. The lower respiratory tract is comprised of the larynx, trachea, bronchi and lungs.

Nose

The nose is the externally visible part of the respiratory system. It forms the part of the face of the animal extending from the transverse level of eyes to the rostral end of the head. Exteriorly it is formed by the bones, muscles, cartilages and the skin. Internally it encloses the nasal cavity which is lined by the mucous membrane. The dorsal part of the nose (roof of the nasal cavity) is termed as dorsum nasi. It is formed by the nasal and frontal bones of the skull. The lateral parts are formed by the incisive, maxillary, lacrimal and zygomatic bones. Covering these bones externally is a muscular layer formed by the facial muscles running over these bones and acting on the upper lip and nostrils. The muscular layer is externally covered by the skin. The skin on the nose bears fine hairs except at the tip of the nose that is formed by the nostrils. The rostral end of the nose is formed by nostrils which provide entrance to the nasal cavity. The nostrils are supported by cartilages which are the dorsal and ventral extensions of the cartilage of the nasal septum. The main aim of the nose is to support and protect the nasal cavity.

The digestive system consists of organs concerned with prehension and digestion of food, absorption of digested food and expulsion of unabsorbed food. The digestive system extends from lips to the anus and consists of the primary and accessory organs of digestion. The primary organs include the mouth, pharynx and the alimentary canal (gastrointestinal tube) in that order of sequence. The accessory organs include the tongue, teeth, salivary glands, liver and pancreas. The alimentary canal extends from pharynx to anus and consists of the oesophagus, the stomach, the small intestine and the large intestine. The wall of the alimentary canal consists of, from within outward, the mucous membrane, the muscular layer and the serous layer forming the visceral peritoneum.

Mouth

The mouth is bounded dorsally (roof) by hard palate and behind that the soft palate, ventrally by the body of the mandible along with the mylohyoideus muscles and laterally by the cheeks. The entrance of the mouth cavity is bounded by the lips and it is called as rima oris, while its caudal opening into the pharynx is called as aditus pharyngis. The cavity of the mouth (cavum oris) is subdivided into two parts by the teeth and alveolar processes (the alveoli) of the mandible. The inner part i.e. the space within the teeth and alveoli is termed as mouth cavity proper, while the space between the cheeks and lips and the teeth and alveoli is called the vestibule of the mouth. The mucous membrane of the mouth cavity is continuous with the margin of the lips and the common integument and caudally it continues with the mucous membrane of the pharynx.

A branch of anatomy that deals with muscles and their accessory structures is called as myology and the body system concerned with myology is called muscular system. The muscular system is composed of specialized cells called muscle fibres because of their shape in the form of fibre or spindle. The plasma of these cells contains myofibrils which are contractile elements that cause contraction of muscles in a definite manner under the influence of a stimulus which is a unique property of muscles. These cells together constitute the distinctive muscular tissue.

Classification of muscles

Based on their morphological and functional characteristics, there are three types of muscles in the body.

Smooth muscle: The muscular tissue in this type of muscles is constituted by masses of spindle shaped cells and they occur mainly in the walls of hollow visceral organs including glands, the vessels, eye ball and hair follicles. These are nonstriated muscles. Contraction in these muscles is weak but sustained and mostly consistent and rhythmic. The action of this muscle is involuntary i.e. not under the will.

Cardiac muscle: The muscle fibres of this muscle are arranged in irregular masses and they form cross striations. The ends of some fibres come in contact with the sides of others forming a kind of syncytium. This is a striated muscle and its action is involuntary. This muscle occurs only in the wall of the heart and the immediate proximal ends of the major blood vessels like aorta, venacava and the pulmonary veins.

A branch of anatomy that deals with joints or articulations in the body is termed as arthrology or syndesmology. A joint is an articulation or union formed by two or more bones or cartilages with the help of connective tissue. To form a joint, the long bones meet at their extremities e.g. joints of fore limb and hind limb. Joints of flat bones are formed by their borders or edges e.g. joint of bones of skull, the cranial and facial bones. The short bones and irregular bones form a joint by uniting at any of their parts or surfaces e.g. intervertebral joints.

Classification of joints

Joints are classified on the basis of their structure i.e. the kind of tissue material by which the bones are united, the other constituent parts that help in uniting the bones and the kind of movement performed at that joint. On this basis, the following three major types of joints exist in the skeleton of animal body.

In domestic animals, skeleton (Fig. 1-1) is a framework that is formed of bones, cartilages and ligaments binding together giving the specific shape, form and support to the body. In addition, the skeletal framework surrounds the major cavities of the body giving protection to other soft visceral organs of the body. There are three major parts of skeleton in the animal body (1) the axial skeleton (2) the appendicular skeleton and (3) the splanchnic skeleton.

The axial skeleton consists of the skull, vertebral column, ribs, and sternum. The appendicular skeleton consists of the bones of fore limb and hind limb

The splanchnic skeleton includes some of the bones embedded in the substance of visceral organs in the body such as os cordis in ox and os penis in dog.

Bones

The bones forming the skeleton of the body are of different shape, size, form and the constituting tissue material. The number of different types of bones of any species may differ in various stages of the growth of the animal. So also the numbers of various bones differ from species to species.

Mole

A mole is defined as the amount of chemical substance containing exactly 6.022 140 76 × 10₂₃ numbers of constitutive particles such as atoms, ions and molecules. This number is a fundamental constant named Avogadro’s number in honor of Italian scientist Amedeo Avogadro. This constant is properly reported with an explicit unit of “per mole,” and rounded version being 6.022 × 10₂₃/mol.

This definition has been adopted since November, 2018 revising the old definition based on the number of atoms in a sample of pure ₁₂C weighing exactly 12 g. Thus, as per the definition, 1 mole of any element contains the same number of atoms as 1 mole of any other element. However, the mass of 1 mole of a particular element is different from that of another element.

Molar mass

Molar mass of an element (or compound) is the mass in grams of 1 mole of that substance, a property expressed in units of grams per mole (g/mol).

Principle

Different ions migrate at different speed dictated by their size and by the amount of charges they carry. As a result, different ions can be separated from each other by electrophoresis. The magnitude of the electric force F exerted on the charged particle when subjected to electrophoresis is product of the charge q of the particle and the electric field E generated between the two electrodes.

Acid and alkaline phosphatase enzymes are among the extensively studied phosphatases and classified so because of their optimum activities in acid and alkaline pH ranges, respectively. Acid phosphatase is predominant in acid soil while alkaline phosphatase is dominant in alkaline soils. The colorimetric assay of phosphatase is based on release of p-nitrophenol from hydrolysis of p-nitrophenyl phosphate byaction of phosphatase enzyme in buffered soil solution (buffer pH 6.5 for acid and 11 for alkaline phosphatase assay).

Principle

β-Glucosidase (BG) plays a major role in the degradation of soil organic matter and plant residues. It catalyzes the hydrolysis of β-d-glucopyranosides, the final, rate-limiting step in the degradation of cellulose, the most abundant polysaccharide providing simple sugars for the soil microbial population. BG has been shown to be sensitive to changes in soil and residue management as well as an early indicator of changes in SOC before these changes are reflected in total or organic C analyses. The method is based on colorimetric determination of the p-nirtrophenol released by β-glucosidase when soil is incubated with buffered PNG solution and toluene.

Fluorescein diacetate (3’ 6’-diacetyl-fluorescein) is a fluorescein conjugated to two acetate radicals. This colourless compound is hydrolysed by both free (exoenzymes) and membrane bound enzymes, releasing a coloured end product, fluorescein. This end product absorbs strongly in the visible wavelength (490 nm) and can be measured by spectrophotometry. The enzymes responsible for FDA hydrolysis are plentiful in the soil environment. Non-specific esterases, proteases and lipases, have been shown to hydrolyse FDA. The hydrolysis of FDA by soil enzymes can be estimated by methods of Adam and Duncan (2001).

Biological activity or index of a soil is the function of number of organisms present in soil and their physiological efficiency. The rate of respiration can be used as an index of the biological activity of soil as it reflects the physiological efficiency of the organisms. Monitoring of dehydrogenase, which are respiratory enzymes and integral part of all soil organisms, will give a measure of biological activity of soil at a given time.

During respiration biological oxidation of reduced compounds occur which is catalyzed by dehydrogenase. In the process TTC gets reduced to a pink coloured compound Triphenyl formazan (TPF) which can be quantitatively extracted by methanol and measured colorimeterically. The soil dehydrogenase activity in the current experiment was measured as per the methods of Cassida, (1977).

Enumeration of microbial flora present in soil is done by dilution plating technique. The technique is based on the fact of diluting the source inoculums to the extent that after plating the known volume of inoculums on appropriate media, the colonies will appear in countable range and each colony will be assumed to have been derived from a single cell/colony forming unit. Three numerically dominant groups of soil microbes are bacteria, actinobacteria and fungi. The most commonly used culture media for their enumeration include

Nutrient Agar-for heterotrophic bacteria,

Actinomycetes isolation Agar or Kenknight and Munaier’s agar for actinobacteria

Martin’s Rose Bengal Agar for fungi.

Analytical methods for study of soil microbes

The analytical techniques for study of soil microbes are grouped under the following heads :

• Determination of form and arrangement of microorganisms in soil
• Isolation and characterization of soil microorganisms
• Detection of microbial activity in soil
• Determination of microbial biomass.

For the above, the different analytical methods used are described below.

This procedure uses Diethylene Tri-amine Penta Acetic acid (DTPA) as the chelating agent, which combines with free metal ions in soil solution and forms soluble complexes.

The hot water soluble method is the most common method to estimate available B content in soil

Reagents

1. Azomethine-H: Dissolve 0.45 g azomethine-H and 1.0 g L-ascorbic acid in about 100 ml de-ionized or redistilled water. If solution is not clear, it should be heated gently on water bath at about 30ºC till it dissolves. A fresh solution should be prepared every week and kept in a refrigerator.
2. Buffer solution: Dissolve 250 g ammonium acetate in 500 ml de-ionized or redistilled water. Adjust the pH to about 5.5 by slowly adding approximately 100 ml glacial acetic acid, with constant stirring.
3. EDTA reagent (0.025 M): Dissolve 9.3 g EDTA in de-ionized or redistilled water and makes the volume up to 1 litre.
4. Standard Boron solution: Dissolve 0.571 g boric acid (H₃BO₃) AR grade in a small volume of deionized water and make volume to 1000 ml to obtain a stock solution of 100 mg B/L.

In this method, plant available sulphur (S) is extracted with 0.15% CaCl₂ and the soluble sulphate is estimated on a colorimeter.

In soil system, potassium (K) exists as water soluble, exchangeable, non- exchangeable and lattice K. The plant available K refers to the sum of water soluble and exchangeable K. Estimation of available K is usually done using neutral normal ammonium acetate as the extractant.

Estimation of plant available phosphorus content in soil has been attempted using several extractants. However, Bray’s reagent and Olsen’s reagent are the two common extractants used in India. Bray’s reagent (0.03 N NH₄F and N HCl) is used for acid soils, where, the fluoride ion complexes with Fe and Al in soil and thus releases the bound P. The Olsen’s reagent (0.5 M NaHCO₃) is used for neutral-alkaline soils.

This is a distillation method proposed by Subbiah and Asija (1956), where the soil is distilled with alkaline KMnO solution. The ammonia liberated is trapped in boric acid and then determined by titration against a standard acid. This procedure estimates the easily mineralizable nitrogen in soil.

Soil respiration is a good estimator of overall biological activity and thus is often used for describing soil quality. Soil respiration is estimated as the flux of CO₂ emitted from the soil surface. It represents the sum of CO₂ produced by root respiration and by heterotrophic decomposition. This procedure is a laboratory method where soil is incubated for a given period of days and the released CO is trapped by sodiumhydroxide (NaoH). This is also known as alkali trap method.

Particulate soil organic matter (POM) is one of the important soil organic matter (SOM) pools and matches the characteristics of a slow, decomposable or stabilized SOM pool. The POM fraction is considered as one of the indicators to changes in management and thus has been used in soil quality assessment studies.

Organic carbon present in soil can be very labile, labile or non-labile. This procedure measures the extent of lability of soil organic carbon through relative degree of oxidation by K₂Cr₂O₇.

Labile fractions of soil carbon (C) refer to the fraction of soil carbon that are easily altered by microbial activities. These fractions serve as sensitive indicators to changes in management induced soil quality and are grouped under active soil C pool. The method described by Weil et al. (2003)estimates changes in biologically active soil C using a dilute KMnO . This is an improvement over the method used by Blair et al (1995), where a very high concentration (0.333 M) of KMnO₄ was used.

This is one of the commonly used method to estimate organic carbon content in soil. In this wet digestion method, soil organic matter is oxidised with a mixture of K₂Cr₂O₇ and concentrated H₂SO₄ utilizing the heat of dilution of H₂SO₄. The unused K₂Cr₂O₇ is back titrated with ferrous sulphate or ferrous ammonium sulphate.

In dry combustion technique, the combustion of soil sample (about 250 mg and mm sieved) is carried out at high temperature (about 1000 ºC) through feeding in an inert boat in the electric furnace. The CO₂ produced from the oxidation of carbon present in the soil sample at high temperature is carried towards non dispersive infrared detector (NDIR) by a carrier gas where it is quantified and total carbon content is determined.

Wet sieving procedure separates soil into different size aggregates. However, for comparing the aggregate size distribution among management treatments and soil types, it is convenient to use indices. Mean weight diameter (MWD) and water stable aggregate percentage are some of the common indices used in aggregate stability studies.

Mean Weight Diameter (MWD)

This index was proposed by Van Bavel (1949) and is being used widely. The entire soil sample is passed through an 8 mm sieve prior to analysis and is subjected to wet sieving procedure as described before. The parameter which Van Bavel (1949) called the MWD is equal to the sum of the product of (a) the mean diameter (d ) of each size fraction and (b) the proportion of the total sample weight (w ) occurring in the corresponding size fraction. The summation is carried out over all ‘n’ size fraction including the one that passes through the finest sieve.

Thisprocedure estimates aggregatestabilitytoimpact ofraindropsand water erosion. Wet sieving procedure was originally proposed by Yoder, 1936. Later, the procedure was modified byKemper and Rosenau, 1986.

Equipment

1. Yoder type sieve shaking electrically operated machine (3/4 inch stroke at 30 strokes per minute)
2. Concentric sieves of various sizes of pores to fit properly into the machine.
3. Weighing balance

Aggregation is one of the basic physical characteristics of soil. Under natural conditions, primary soil particles, particularly clays, tend to join together to form secondary units called aggregates. Size distribution of soil aggregates is important because the size of the aggregates determines their susceptibility to water and wind erosion. Also, their size is important in determining the pore size distribution and regulating pore space in cultivated soils.

There are a variety of procedures being in use to test the stability of aggregates and aggregate size distribution. However, choice of the method for analysis of aggregate size distribution is determined by the purpose of analysis. The developed measures are relative and purpose specific. This chapter discusses dry aggregate analysis.

Aggregation is a direct indicator of soil structure. Ahealthy soil is considered to have well aggregated structure. Though not routinely followed during soil testing and chemical analysis, aggregation is one of the major indicators of soil physical health. Wet sieve method (Yoder, 1936) is usually followed for soil aggregate analysis. This involves gently raising and lowering a nest of sieves in water to assess the stability of aggregates to rapid wetting. However, the Yoder’s wet sieving method has been modified to make it simpler and currently, the method of Kemper and Rosenau, 1986 is being commonly used in research laboratories. One of the pre-requisites in these methods is the availability of wet sieving apparatus and further, these methods are laborious and time consuming. On the other hand, the method described by Cambardella and Elliott (1993) is considered very ideal because of its simplicity and is being widely used for aggregate bound soil carbon fraction study. The soil sample is wet sieved with a series of three sieves (2000, 250 and 53 micron size).

Soil bulk density (BD) or apparent density is determined in -situ by core method. Stainless steel or metallic cores of cylindrical shape with known height and diameter are inserted into the soil till the rim of the core. The soil core is then excavated and the soil sample is oven dried to get the dry weight. The ratio of the oven dry weight to the total volume of the core gives the bulk density of the soil.

Soil texture refers to the relative distribution of sand, silt and clay on a sample of <2 mm size. It is an intrinsic soil property and basically refers to the particle size distribution in a soil. A number of physical characteristics and chemical properties of a soil are influenced by texture or particle size distribution. To estimate the content of sand, silt and clay particles, the soil samples are first brought into a dispersed state by mechanical and chemical treatments so that different binding agents are removed. In the dispersed soil samples, the soil particles settle down at a differential settling rate according to their size. In laboratory, the common methods for analysis for particle size distribution are : Buyoucos hydrometer method and International pipette method. In this chapter, the procedure of Buyoucos hydrometer method is described.

Principle

This method is based on the principle of variable rate of settling of particles as per their size and as described by Stokes’Law. Large particles shall settle fast. The measurement of density of the suspension is done at different times using a hydrometer (ASTM 152H-Type hydrometer is preferred). The measurement in this hydrometer is based on a standard temperature of 20 _oC and a particle density of 2.65 Mg m_-3 and the units are expressed in grams of soil per liter.The principal source of error in this procedure is the incomplete dispersion of soil clays as the clay particles are cemented by agents such as organic matter, CaCO and iron oxides. Incomplete dispersion results in low values for clay and high values for silt and sand. Thus, soils with higher organic matter (>1.0%) should be first treated with 6% hydrogen peroxide (H₂O₂) for removal of organic carbon. The rate of sedimentation is also affected by temperature and the density of the dispersing solution.

Exchanges sodium (Na+) is animportantparameter forsalt affected soils. This is estimated by extraction with ammonium acetate and then measuring the extract inflamephotometer.

Reagents

1. Neutral normal ammonium acetate solution (1 N CH₃COONH₄ of pH 7): Dissolve 77.09 g of ammonium acetate in distilled water and make up the volume to one litre. Adjust the pH of the solution to 7 with ammonia solution or acetic acid.
2. Standard solution of Na (500 mg/L Na): Dissolve 1.271 g of NaCl in distilled water and make up the volume to one litre.
3. Working standard solution of Na (100 mg/L): Dilute 200 ml of 500 mg/L stock solution to 1000 ml.

Ion exchange is a reversible process by which a cation or anion held on the solid phase is exchanged with another cation or anion in the liquid phase. Soil mineral and organic colloidal particles have negative valence charges that hold dissociable cations. The power to retain cations at the surface of soil colloids is referred to as the cation exchange capacity (CEC). Cation exchange is a reversible reaction in soil solution, dependent upon negative charges of soil components arising from permanently charged or pH-dependent sites on organic matter and mineral colloid surfaces. The CEC is the sum of cations held by a kilogram of soil and is expressed in Cmol (p₊)/kg soil. In most agricultural soils, the cation-exchange capacity (CEC) is generally higher than the anion exchange capacity (AEC).

Electrical conductivity (EC) is a useful indicator of soil salinity. Relationships have been developed between EC and salinity classes for a 1:1 soil: water suspension. With increase in salt content in a soil, the EC increases. Thus, for salt affected soils, measurement of EC is vital as accumulation of salts in the root zone is detrimental to plant growth. EC is a measure of the ability of a soil solution to conduct electrical current.

Equipment and Reagents

1. Digital Conductivity meter
2. 0.01 M potassium chloride (KCl) solution: Dissolve 0.7456 g dry KCl in distilled water and make up the volume to 1.0 litre. This solution has an electrical conductivity of 1.413 dS m_-1 at 25ºC.

Soil pH is the measure of hydronium ion (H₃O⁺) or more commonly hydrogen ion (H⁺) activity in the soil suspension. Soil pH can be measured in soil-water suspension, saturation extract or in 0.01 M CaCl₂ solution. Saturation extract is used to identify specific soil problems such as salinity or sodicity. Measurement of pH using 0.01 M CaCl₂ solution gives more reproducible results than measured through soil-water suspension. It also provides a good approximation of field conditions as the electrolytic composition of 0.01 M CaCl₂ is considered similar to that of soil solution. However, among the three methods, soil-water suspension is most commonly used in research laboratories as well as for fertilizer recommendation as it is simpler and more convenient.

Soil sample collection is the first and most important step for soil quality analysis. A soil sample collected with due care and proper procedure is essential for getting reliable result of the analysis. The sample should represent the field and for practical purposes, an area of about 0.5 hectare is considered as one sampling unit. In case of conspicuous variation observed in the field in terms of texture, colour, slope etc, separate samples should be collected from areas differing in these attributes. In other words, sample should be collected from an area in the field with similar characteristics as seen through normal visual observation. Sampling should thus be avoided from areas like near trees, permanent vegetations, manure pits, manure piles or recently fertilized fields.

Soil Sampling procedure

1. For a particular field unit, one composite soil sample is prepared out of the samples collected from 7-8 spots distributed across the field unit. The entire field is covered by moving in a zig-zag manner from each sampling spot.
2. For each sampling point or spot, scrapping off the surface litter such as grasses or crop residues is done. Then, a “V” shaped pit of 15-20 cm length and of equal width is made. Depth of sampling depends upon the requirement and on the type of crop. For field crops like paddy, wheat etc, samples are collected from 15 cm depth. From the “V” shaped pits, take outslice of ½ inch thickness from both sides of the exposed pit surfaces from top to bottom.
3. The soil samples so collected from the 7-8 points are mixed thoroughly by hand and then is reduced to about 500 g by quartering process. The quartering process consists of a procedure in which the entire soil mass is divided into four quarters; the two opposite ones are discarded and the remaining two are remixed. This process is repeated till about 500g soil is obtained.

1. Introduction

Persian walnut is of great importance across worldwide where nuts are harvested from wild stands, backyard gardens and rarely from commercial orchards that vary considerably in their size due to degree of management. The walnut is an old tree cultivated before the Greek age and appreciated for centuries for its fruits, wood and other parts utilized in several ways. Before the discovery of the phenomena of allelopathy, someone considered the walnut an un-healthful tree, since peasants used to wake up with migraine or fever after they had slept under a walnut tree. Some others considered the walnut a cursed tree, and in fact in Italy an old walnut tree growing in Benevento, has long been considered the place where the witches used to dance during the night of Saint John and, therefore, that tree was named “Tree of the Witches” (Avanzato, 2010).

Nuts are collected for home consumption, sale at local roadside stands and markets and for shipping to cities. Walnut trees are further utilized for their high quality timber to make a wide array of products. The leaves, bark and other plant parts are used for medicinal remedies and trees are grown and maintained for soil conservation purposes (Mamadjanov, 2006b). A diverse mix of seedlings, local selections, and advanced cultivars are grown for nut production, many with unknown origins, including some of which are the direct result of selection from the wild.

1. Introduction

The modern cultivated strawberry (Fragaria x ananassa Duch.) is one of the most delicious, refreshing and soft fruits of the world. Worldwide it is also the most widely distributed fruit-crop due to its genotypic diversity, highly heterozygous nature and broad range of environmental adaptation. The plant is cherished in gardens and in commercial fields for its beautiful red fruit that has a tentalizing aroma (Sharma, 2002; Sharma and Sharma, 2004). Being a rich source of vitamins and minerals coupled with delicate flavour, strawberry has now become an important table-fruit of millions of people around the globe. It is amongst the few fruit crops, which gives quicker and very high returns per unit area on capital investment, as the crop is ready for harvesting within 6 months of planting. However, due to several technological advances in its cultivation like, introduction of day-neutral cultivars, and protected cultivation, strawberries remain available as fresh fruit throughout the year.

At present, strawberry is grown in wide climatic zones, extending to temperate, Mediterranean, sub-tropical and taiga zones (Sharma, 2002; Sharma and Sharma, 2004). Its cultivation is influenced by the specific regional adaptations due to critical photoperiods and temperature requirements and thus its cultural systems are highly variable. Due to the constant efforts of strawberry breeders, the world-wide interest for strawberry cultivation has boosted its production tremendously, which has resulted in widespread popularity of strawberry in the last 50 years.

1. Introduction

Quince is a highly nutritious underutilized fruit of sub-Himalayan region which has great economics and medicinal potential and it belongs to the group of the oldest cultural plants. Quince is known with different names, Urdu name ‘Bahee Dana’, Farsi name ‘Beh’, Greek name ‘Strythion’, Kashmiri name ‘Bamchunth’ and Hindi name ‘Bihi’ (Torkelson, 1995, Khoubnasabjafari and Jouyban, 2011). Quince is very much like pear in shape and gets a golden yellowish outer layer when mature. Quince fruit is a valuable dietary product and even then it less suitable for the direct consumption (Hricovsky et al., 2003) and for that reason they are mostly used either cooked (Alvarenga et al., 2008) or preserved (Kyzlink, 1990). However, as compared with other species, this fruit tree species is less popular throughout the world. Its predisposition to turning brown and decay is a limiting factor in the long-term storage of this fruit (Gunes, 2008). The nutritional value of the fruit is high (Bucsek et al., 1996; Kopec and Balik 2008). It is well known that quince fruit shows antioxidant effects (Silva et al., 2002) which result from the presence of a number of polyphenolic substances (Fattouch et al., 2007), e.g. flavonoids quercetin, rutin, kaemp ferol etc. (Silva et al., 2005), and also from increased levels of vitamin C (Tetera, 2006). Quince has the important nutritional and flavor composition more than many fruits. Vitamin C content of quince is nearly two times higher than in apple (Biro and Lindner, 1999).

1. Introduction

Plum is one of the important stone fruit crops of temperate region throughout the world. The Japanese group of plum is native to China but was domesticated in Japan and subsequently was introduced to different parts of the world. In India, Japanese plums were introduced by European settlers and missionaries around 1870. Plum grows well in areas ranging from 900 m to 1600 m above mean sea level.

1.1. Uses

Among deciduous fruits, plum is widely distributed in the world, because of wider adaptability among different species and cultivars of plum to varied agro-climates and soil conditions. Plum is consumed as dessert, dried and processed products. Plum fruit is considered best to overcome the jaundice. Its fruit use for a preparation of juice and squash. It ranks next to peach in commercial production. It is widely cultivated in several countries in Europe, Asia and in the America.

1. Introduction

The pistachio nut tree, Pistacia vera L. has been extensively cultivated since ancient times in the Middle Eastern and Mediterranean countries; in India, Afghanistan. The climatic conditions favorable to pistachio culture are similar to those for almond and olives, and prevail in extensive regions of the western United States also. Pistachio is a nut which comes originally from Iran and has the history of 4000 to 5000 years. Pistachio is a drought-tolerant, slow-growing, deciduous tree that reaches to a height of 30 ft. The tree survives in arid regions where summers are hot and the winters cold. Rocky, poor terrain with little rainfall is no problem as long as there is good drainage. The fruits grow in clusters. Although called a nut, botanically, pistachios are actually drupe. The pistachio consists of a bone ivory shell that encases the edible meat portion, called the seed.

1. Introduction

Pecans (Carya illinoensis) are one of the most desirable trees to plant in home yards. Their size and natural beauty as a shade tree and its capacity to produce a nutritious food make them valuable. Pecan is the most widely cultivated and the most economically important member of the North American hickory group. Beyond its native range, pecan is commercially grown from the southeastern USA to the south-west (California) (Hanna, 1987, Peterson, 1990, Harlow et al., 1991). Pecan is from an Algonquian word, meaning ‘a nut requiring a stone to crack’. In Mexico, pecans and walnuts share the same Spanish name, neuz, which is a cognate of the English word nut. In the USA, pecan is considered the queen of nuts because of its value both as a wild and as a cultivated nut and acceptable quality of meats (Woodroof, 1979). Pecan is also considered as ‘queens of nuts’ because of its value both a wild and as a cultivated nut and acceptable quality of meats. As compared with other nut fruits which have been grown since time immemorial, pecan has been under cultivation only for about one hundred years but has gained enormous popularity.

1. Introduction

Pear is among the oldest of the world’s fruit crops, figuring in both Bible and the tales of Homer. They are by far the most important of the deciduous tree fruits, are widely grown in temperate and increasingly, in tropical regions, and figure prominently in world trade. The fruit is primarily grown for the fresh fruit market, which is much more remunerative than that for processing.

Pear (Pyrus communis, Rosaceaea family), is a deciduous fruit, grown under a wide variety of climatic regimes, ranging from cold dry temperature hilly conditions to warm humid subtropical conditions. It is grown in many places of India like Jammu and Kashmir, Himachal Pradesh, Karnataka, Tamil Nadu, Kerala, Utter Pradesh. Sandy pear plants were brought to Amritsar by travellers from China who settled at Harsha Chinna village near Amritsar about 2,500 years ago. The Kashmir valley represents one of the major Pear (Pyrus communis.L.) growing areas of the world. Pear was introduced in India from China. The time at which Pear was introduced to Kashmir is not precisely known, however Yukteshwar Kumar writes in his book “A History of SinoIndian Relations” that peach and pear came to India during the early years of the Eastern Han period (25 AD to 220 AD). This pre historic fruit has been under cultivation both in Europe and Asia for about 3000 years (Hedrick et al., 1921). Between 16^th and 18^th centuries, many European writers travelling Kashmir noted fantastic royal gardens of the Mughals, mentioning the abundance of peach, pear, plum and cherry trees, with fountains spouting cold, icy water. Pear (Pyrus communis. L), originating from the German word ‘peer’ meaning fruit, is a fascinating fruit steeped in rich history. This fruit is known as ‘tang’ in Kashmiri language. Low chill requiring pears are being cultivated in plains of North India. Cultivars requiring high chilling hours (900-1000) are cultivated at higher hills of Jammu and Kashmir, Himachal Pradesh and Uttarakhand. Majority of the Pear production is actually limited to Kashmir however, low chilling cultivars grow in subtropical regions.

1. Introduction

The peach [Prunus persica (L.) Batsch] is an important fruit crop of temperate regions, all over the world. Important centers of commercial production usually lie between latitudes 30° and 45° North and South. At higher latitudes, minimum winter temperatures and spring frosts are the limiting factors. The peach flower bud is hardy and can tolerate minimum temperature of about -23 to -26° C. Its northern range is extended where minimum temperature are ameliorated by large bodies of water, as western continental seaboards, the great Lakes in the U.S.A. and Canada and the Caspian and Black seas of Eurasia. Mid continental areas are characterized by lower winter temperatures and usually more severe spring frost hazards. Most peach cultivars require 500 to 1000 chill hours below 7.2° C to foliate and bloom. At lower altitudes, the winter requirement is not met. The peach with its smooth skin mutant is known as nectarine. Nectarines can be used in the same way as peaches, and may be considered as substitutes for peaches.

1. Introduction

Olive (Olea eurpaea L.) is an evergreen species commonly grown in the Mediterranean basin. The oil extracted from the fruit is a predominant component of the ‘Mediterranean diet’, which has documented heart- and cancer-protective benefits. These derive from the lipid composition and from biologically active molecules that accumulate during olive fruit development. The oil can reach up to 30% of the total fruit fresh weight at full ripening and is highly present in the mesocarp and at lower levels in seeds. Oil increase reaches a plateau in pulp after varaison. A marked triacylglycerol accumulation in seed and pulp occurs after endocarp lignification, when about 40 mg of oil per fruit per week can be synthesized. The fatty acid profile of the oil accumulating in the fruit is important in relation to its nutritional properties. The main fatty acid is oleic acid (C18:1), which represents about 75% of total fatty acids, followed by linoleic (C18:2), palmitic (C16:0), stearic (C18:0) and linolenic (C18:3) acids (Martinelli and Dandekar, 2014).

The olive, Olea europaea L, is the only species of the botanical family Oleaceae with edible fruit. The tree blooms with small white flowers in May, and fruit set begins almost immediately afterward in May or June. Ripening is complete toward the end of November or December, depending on the variety. Its cultivation began more than 6000 years ago in the Middle East, and spread west on both sides of the Mediterranean Sea.

1. Introduction

Kiwifruit (Actinidia deliciosa Chev.) is among the most recently domesticated of all fruit plants. The kiwifruit of international commerce are large – fruited selections of Actinidia deliciosa Planch and Actinidia deliciosa (A. Chev.) C F Liang et A. R. Ferguson, belongs to family Acitinidiaceae. A. chinensis has been cultivated commercially for little more than 20 years, however, A. deliciosa for about 70 years. This is known as “China’s miracle fruit” and “Horticulture wonder of New Zealand”. Although this fruit vine originated in China, yet its full economic potential was exploited by the New Zealanders, which accounts for over 70 per cent of world trade (Franco, 1990). Kiwifruit has completely replaced the older name of ‘Chinese gooseberry’. In China, ‘Mihoutao’ (monkey peach) is used for all Actinidia species, but as A. chinensis and A. deliciosa are by far the most important economically of the various Actinidia species in China, they are often simply referred to together as Mithoutao. More explicitly, the two species are sometimes referred to together as Zhonghua (Chinese) Mihoutao. In other countries of the world, its cultivation gained momentum after 1960s and now is cultivated on a commercial scale in USA, Italy China, Japan, France, Germany and Australia.

In India, it was first introduced in 1960 at Lalbagh garden, Bangalore, but the plant did not come into bearing due to lack of chilling requirement during winter for its bud break. Later on, in 1963, it was introduced in Shimla hills, where the plant came into bearing in 1969. In order to assess the performance of kiwifruit cultivation in different agro-climatic conditions of state its plantation were done at different locations. After evaluation, this fruit was recommended for commercial cultivation in mid and low hills of the state. It can also be grown in mid hill of Jammu and Kashmir, Uttrakhand, Sikkim, Arunachal Pradesh, Meghalaya, Nagaland and Nilgiri hills of south India.

1. Introduction

Hazel nut also called as European filbert or Bhotia badam (Corylus avellana L.). Apparently the word ‘filbert’ has been derived from Middle English which in turn was a derivation of Philbert - the Saint Philibert, whose feast day in late August coincides with the ripening of the nut. Others believe that the term filbert came from the nut’s long husk being referred to as a full beard in Old English. The name “Hazel” comes from the Anglo-Saxon word for bonnet, “Haesel”; this probably derives from the greek “korys” (as in Corylus), for helmet or hood. The term “filbert” may have derived from “full beard”, descriptive of the long, leafy husk. The scientific name avellana derives from the town of Avella initially and was selected by Linnaeus from Leonhart Fuchs’s De historia stirpium commentarii insignes (1542), where the species was described as “Avellana nux sylvestris” (“wild nut of Avella”). Hazelnut is the 4^th economically important nut crop (748,000 MT/yr) of world after cashew, almond and walnut.

1. Introduction

Cherry is the common name of several Prunus species that include sweet cherry (P. avium), tart or sour cherry (P. cerasus), Duke cherry (hybrids between P. avium and P. cerasus). Sweet cherries are believed to have originated from the area between the Black and Caspian seas of Asia Minor. Birds probably carried the seed to Europe prior to human civilization. Cultivation was probably started by the Greeks and continued by the Romans. Its distribution and naturalization have proved extensive, for it is now found wild in areas as widely spaced as northern India, the southern plains of Europe and even on isolated islands such as Madeira. Climatic conditions are the most important limitations on cherry production. Being a deciduous tree, it needs a period of dormancy, which is initiated in temperate regions mainly by a fall in average temperatures in late autumn (Mir et al., 2017). Sweet cherry is grown almost everywhere in the temperate zone. It is a composite genus. Due to its origin, this genus needs warm and light conditions, but can also endure cold winter weather. It requires 550-600 mm precipitation annually. Sweet cherry prefers medium light (pH= 5.5–7.5), deep-layered soil, which is rich in nutrients. The root system of sweet cherry requires aeration and cannot tolerate slack water (Simon, 2004). Many areas of the world, although environmentally suited to cherry production, are currently unsuited to profitable cherry production due to high perishable nature of the fruit.

1. Introduction

Apricot is among the highly nutritious fruits of Rosaceae family and appreciated for its health promoting contents. Studies have shown that apricot fruit is effective in preventing stomach ulcers and oxidative damages of living systems (Enomoto et al., 2010; Miyazawa et al., 2006). Apricot tree belongs to genus Prunus in the Rosaceae (rose) family. Apricot (Prunus armeniaca) is categorized under ‘stone fruits’, along with peaches, plums, almonds, and some cherries, due to its seed being enclosed in a hard, ‘stone’ like endocarp. This fruit is native to temperate Asia, first discovered growing wild on the mountain slopes of China, and long cultivated in Armenia later.

Throughout its history, apricot has been noted for delicious flavor, delicate velvety fruit surface, and early flowering and fruiting. The name apricot is a corruption of the Arabic and Greek al-paraecox which means the early fruit. The old spelling ‘apricock’ is retained in English until the 17^th century. The fruits are mainly consumed fresh but are also cooked and often stuffed. The high sugar content makes them suitable for drying. In china they were also preserved by salting and smoking. Other uses include fruit leathers, nectars, and liquors (Janick, 2005).

1. Introduction

The cultivated apple (Malus × domestica Borkh.), is the most important fruit in temperate regions of the world. Apples were frequently used in different mythologies as symbols of immortality or reincarnation. One of the most popular stories attached to apple is that of Adam and Eve, who ate the forbidden fruit from the tree of good and evil which was in the center of the garden of Eden. The Latin word Malus has two meaning i.e. apple and evil, which has probably stemmed from this story from bible. In Greek mythology, mother earth or Gaia, presented a tree with golden apples to Zeus and his bride Hera on their wedding day. These golden apples were later indicated with many stories of love, bribery and temptation ranging from the abduction of Helen of Troy to the defeat and marriage of Atlanta. Goddess Pomona was deity of fruit trees in Rome. In many cultures, garden of paradise and pleasure is symbolized as the place with fruit-laden apple trees. Apple is popular because of its delicious taste and pleasant aroma.