eBook Chapters

The deliberation on the methodology has been made to understand the concept, methods and techniques which have been executed for delineating the study, collection of information, analysis of data, and interpretation of the findings for the revelation of hard evidences and formulation of theories. This chapter deals with the method and procedure used in the study consisting of eight main parts

A. Locale of research

B. Pilot study

C. Sampling Design

D. Item analysis and experts rating for devising scoring technique on dependent variables

E. Empirical measurement of the variables.

This chapter deals with an in-depth description of the methods and procedures employed in conducting the research in an organized manner. The research methodology gives the study’s work plan. The research objectives and theoretical base offer an initial framework for identifying the appropriate methodology to answer the research questions.

The procedures followed were described under the following headings.

5.1 Research design

5.2 Locale of the study

5.3 Content analysis of websites

5.4 Statistical tools used for the analysis of data.

5.5 Preparation of report

8.1 Introduction

After knowing when to irrigate and how much to irrigate, next important thing is how to irrigate the crop field. Methods of irrigation play a very crucial role in deciding how to irrigate the crop fields since all the crops do not require same methods of irrigation. Depending on the sources of water available for irrigation, type of crop to be irrigated, quantity and quality of water to be applied and other economic considerations, methods of irrigation will vary. Irrigation water may be applied to the crops by flooding it on the field surfaces as surface irrigation, by applying it beneath the soil surface as subirrigation, by spraying it under pressure as sprinkler or overhead irrigation or by applying it in drops around the root zone of the crop as trickle irrigation. The commonly used methods of irrigation are described in schematic diagram as follows:

Irrigation Methods

Surface

Sub-surface

Pressurized irrigation

12.1 Surface Irrigation

Surface irrigation method refers to the manner or plan of water application by gravity flow to the cultivated land wetting either the entire field (uncontrolled flooding) or part of the field (furrows, basins, border strips). Most irrigated areas have characteristic land features and differ from those in other areas. Hence, for efficient application of water it is important to select such method of irrigation, which fits one’s own land.

The adoption of a certain method need not be necessarily based on convention or that followed in the adjoining farm. The factors, which determine the suitable method of irrigation are local conditions (soil type - its permeability & water storage capacity; land topography, climate, water availability & water quality), crop type, type of technology, previous experience with irrigation, required labour inputs etc. Good yield of crops can be obtained from irrigated land only if the water is applied judiciously to meet the needs of the plant, but not to cause waste and damage. Irrigation water is applied to cultivated land by the following surface methods of irrigation:

1. 1 Introduction

Irrigation is the artificial application of water needed to the crop field. It is the process of uniformly wetting the root zone depth. The wetting may be done over the land surface or below of it. The application of water in excess to the requirement or accumulation of water in crop field due to rain or unplanned seepage is not considered as irrigation. However, irrigation is supplemental to rainfall. Irrigation water is considered as single most important inputs for crop production.

9.1 Introduction

After knowing when to irrigate and how much to irrigate, next important thing is how to irrigate the crop field. Methods of irrigation plays a very crucial role in deciding how to irrigate the crop fields since all the crops do not require same methods of irrigation. Depending on the sources of water available for irrigation, type of crop to be irrigated, quantity and quality of water to be applied and other economic considerations, methods of irrigation will vary. Irrigation water may be applied to the crops by flooding it on the field surfaces as surface irrigation, by applying it beneath the soil surface as sub irrigation, by spraying it under pressure as sprinkler or overhead irrigation or by applying it in drops around the root zone of the crop as trickle irrigation. The commonly used methods of irrigation are described in schematic diagram as follows

1.1 Introduction
Irrigation is the artificial application of water needed to the crop field. It is the process of uniformly wetting the root zone depth. The wetting may be done over the land surface or below of it. The application of water in excess to the requirement or accumulation of water in crop field due to rain or unplanned seepage is not considered as irrigation. However, irrigation is supplemental to rainfall. Irrigation water is considered as single most important input for crop production.

Object: Methods of measurement of water status and water saturation deficit in plant parts. 

Material required: Leaf samples, leaf borer, water, petridishes, forceps, oven and blotting paper. 

Theory: Plant water status strongly influences plant growth and biomass production through its effects on leaf and root expansion and on photosynthesis. Therefore, measurement of plant water status is an important component of understanding plant processes and maximizing yield under different ecosystems. 

Method: Plant water status can be measured and computed by any one of the following methods

Atoms and molecules are the essential building blocks of every object. The manner in which things are constructed with these basic units is vitally important to understand their properties and their reciprocal interactions. An efficient control of the synthetic pathways is essential during the preparation of nanobuilding blocks with different sizes and shapes that can lead to the creation of new devices and technologies with improved performances. Traditionally nanoparticles were produced only by physical and chemical methods. Some of the commonly used physical and chemical methods are ion sputtering, solvothermal synthesis, reduction and sol gel technique

Basically there are two approaches for nanoparticle synthesis namely the Bottom up approach and the Top down approach. In the Top down approach, scientists try to formulate nanoparticles using larger ones to direct their assembly. The Bottom up approach is a process that builds towards larger and more complex systems by starting at the molecular level and maintaining precise control of molecular structure.

These two different methods highlight the organization level of nanosystems as the crossing point hanging between the worlds of molecular objects and bulk materials.

The top-down approach has been advanced by Richard Feynman in his often-cited 1959 lecture stating that “there is plenty of room at the bottom” and it is ideal for obtaining structures with long-range order and for making connections with macroscopic world. Conversely, the bottom-up approach was pioneered by Jean-Marie Lehn (revealing that “there is plenty of room at the top”) and it is best suited for assembly and establishing short-range order at the nanoscale. The integration of the two techniques is expected to provide, atleast in principle, thewidest combination of tools for nanofabrication.

The Food and Agriculture Organization (FAO) estimates that up to 40% of food crops are lost due to plant pests and diseases annually. Such losses not only threaten global food security but also have the potential to limit food access due to unavailability of food, or sharp increases in food prices. But this challenge is also an opportunity to find new ways to help crops better withstand adverse conditions in a farmer’s field.

19.1 Introduction

In self-pollinated crops, the methods of selection aim at improving individual plants. On the other hand, in cross-pollinated crops they aim at improving a population of plants. A population is a large group of interbreeding individuals. Hence, selection is called population improvement in cross-pollinated crops. Selection in self-pollinated crops results in isolation of pure breeding lines which is the end product. But in cross-pollinated crops, it results in an improved population which is used as the commercial cultivar. Improved inbred lines (also called inbreds) for producing hybrids can be obtained from such populations. Again, like many rounds of selection being practised in different inbreeding generations in self-pollinated crops we are also practising many rounds of selection in cross-pollinated crops. But, unlike self-pollinated crops selection in cross-pollinated crops is followed by intermating to develop a new population before starting another round of selection. One cycle of selection consists of selection of individual/family and intermating of the selected units. Such cycle is repeated till we get the improved population. This method of practising the cycles of selection and recombination in a population is called recurrent selection or population improvement. Frey (1983) prefers the use of recurrent selection for closed populations where no new germplasm is added to the population during selection. Population improvement was used for a group of breeding techniques where all aspects are similar to recurrent selection but with the provision of adding new sources of germplasm whenever feasible. It is only a matter of coincidence that past history of application of recurrent selection has been limited to closed populations, but its basic aim has always been the accumulation of favourable genes, without any restriction on the migration of new genes during the course of selection. Apart from a slight nuance, population improvement and recurrent selection are two titles of the same principle and procedure of manipulating the genetic potential of populations. Recurrent selection can also be applied in self-pollinated crops.

17.1 Introduction

Self-pollinated vegetable, spice and ornamental crops may be heterogeneous. It is exemplified by landrace cultivars. They may have originated from single plant selections but have been grown over a long period of time. Landrace cultivars are a mixture of genotypes, perhaps because of breeders’ lack of appreciation of selection techniques to produce a pure line, spontaneous mutations, chance hybridization, or a combination of factors. Natural selection in landraces has resulted in a cultivar that is well adapted to the local environment. Depending on amount of human selection, differences among genotypes in a landrace cultivar may be small and inconspicuous. On the other hand, a lack of human intervention may mean that differences among plants in a landrace cultivar will be conspicuous. After years of self-pollination, individual plants are homozygous. Selection of such individuals would result in a true-to-type reproduction of their phenotype within the limitations imposed by the environment.

18.1 Introduction

The genetic variability present in populations of self-pollinated vegetable, spice and ornamental crops is rapidly exhausted by pureline selection. For further improvement, genetic variability must be created. This is generally achieved by hybridization. After the cross has been made, F₁ is grown and seeds set on it are harvested. This makes the seed source for raising F₂ population, and so on. The segregating populations from crosses are handled according to a suitable method. The three basic methods available for this purpose are: (1) pedigree method, (2) bulk method, and (3) backcross method. There are also certain modifications of these methods.

18.2 Pedigree Method

Pedigree method is developed from the pureline system and widely used method of plant breeding for self-pollinated crops. A main difference between pedigree selection and mass selection or pureline selection is that hybridization is used to create variability for the base population in case of the former, whereas in mass and pureline selections variability is naturally present in the base populations. The pedigree method was first described by H.H. Lowe (1927). This method predominated the crop improvement programme throughout the world until 1980s. Pedigree method is common in horticultural crops, especially where readily identifiable qualitative traits are involved. Vegetable crops in which pedigree system is used include tomato, cucumber, pepper, pea, lettuce, snap and field beans, all self-pollinated crops. In general, selection is done on F₂ plants. Yield is important in many horticultural plants, but quality is equally or even more important. Quality may be associated with identifiable traits, making the pedigree system ideal for self-pollinated vegetable crops.

An animal breeder usually interested in various economic traits and when wishes to improve them in the herd/flock, he has three general methods at his disposal. Tandom method, independent culling level method and  index selection method.

Tandom Method

It involves selection for one trait at a time and until it reaches at a satisfactory level, and then for a third trait etc. This method is applicable only when the different traits in question are entirely independent or positively genetically associated to each other. Its effectiveness decreases with the increase in number of traits. This method is least desirable of all the methods.

Tandem Selection
Selection is practiced for one trait at a time until satisfactory improvement has been achieved in this trait. Then, selection efforts for this trait are relaxed and efforts are directed towards the improvement of a second, then for a third and so on, until finally each trait has been improved to the desired level. This method is the least efficient of all the three methods, from the stand point of the amount of genetic progress made for the time and effort by the breeder. Efficiency of tandem selection depends a great deal upon the genetic correlation between the traits selected for. If the genetic correlation is positive and desirable, improvement in one trait by selection would automatically result in improvement in the other trait not selected for, and then the method could be quite efficient. Otherwise, if there were little or no genetic correlation between the traits, which means that they are inherited independently, the efficiency would be less. When the two traits are negatively correlated, improvement in one trait is nullified or neutralized by the regression in the other. For example, in poultry, selection for egg number will result in reduction in egg weight, as they are negatively correlated.
Independent Culling Level (ICL) Method
Method of selecting two or more traits but they both need to meet a minimum standard. It is very strict that if the animal didn’t meet one trait it would be rejected. Independent Culling level (ICL) Method selects several characteristics or traits simultaneously.
Breeders set minimum or maximum culling levels or standards or criteria for each trait of selection objectives. Any animal not meeting all criteria is not selected for use in the breeding program regardless of the level of excellence of other traits. With each successive generation of progeny, the minimum quality of each characteristic is raised thus ensuring improvement of these traits. For example, a breeder has a view of the minimum requirements for milk yield, fat yield, and service period for which cows are breeding. The breeder will determine what the minimum acceptable quality for each of these traits will be for progeny to be folded back into her breeding program. Any animal that fails to meet the quality threshold for any one of these criteria is culled from the breeding program. Thereby, it causes the loss of excellent genes from the population for a particular trait.

Objectives • Seminal evaluation and processing • Evaluation of bull fertility on the basis of its seminal characters • To make the Artificial insemination programme successful History Related to Semen Collection • The use of artificial vagina was started by Milovanov for semen collection. Before that method of placing the cotton ball in vagina was used. • AV for Dog was discovered by Amantea in 1914. Milovanov discovered AV for Bull, ram and stallion. Mckenzie discovered AV for boar (1931). • The electroejaculatory method of semen collection was initiated in a bull by Laplaud & Cassou and in ram by Gunn. • Burrows and Quinn (1937) developed the method of abdominal massage and pressure to collect semen in poultry.

2.1 Simple Random Sampling
The simplest and common most method of sampling is simple random sampling. In this procedure, the sample is drawn unit by unit with equal probability of selection for each unit at each draw. It is sometimes referred to as unrestricted random sampling.

2.1 Simple Random Sampling

The simplest and common most method of sampling is simple random sampling. In this procedure, the sample is drawn unit by unit with equal probability of selection for each unit at each draw. It is sometimes referred to as unrestricted random sampling.

2.1.1 Definition

“A sampling procedure which gives equal chance to all possible samples each of size n which can be drawn from a population of size N, is called simple random sampling.”

Progeny testing in India was introduced in the late 1950’s the first sire index in our country was suggested by Krishnan (1956) which was, however, similar to the herdmate and contemporary comparison methods. Robertson and Rendel (1954) stated that an efficient method of sire evaluation should satisfy two conditions: 

There is urgent need to increase the production in order to meet the demand for the exploding population. This could be achieved by applying various modern methodologies in selection and breeding of livestock. Increasing the productivity through genetic improvement requires adequate identification and intensive selection of genetically superior sires. About 93 per cent of the total herd improvement comes from breeding of young bulls from tested sires and only six per cent from selection of dams.
With the advances in artificial insemination and cryopreservation of semen, a sire has a potential of serving 3/4th million cows and producing 1/4th million progenies.
Thus, selection of bulls is of great importance in dairy herd improvement.
For maximising the genetic gain by sire selection, it is essential that the method of estimating breeding values of sires should be unbiased and efficient. The breeding value refers to the average genetic effect of the genes passed on by the individual to its offspring and is estimated to know whether the individual is genetically superior to other individuals or not for the trait concerned.

Since commencement of the settlement of world, the search for food had been uppermost, thereby, practising the conservation of food is a basic need for ensuring its ample supply to human being. The problem of food storage is growing fast day by day with the increase in the population. The existing shortage of protein and caloric requirements will not only be solved by growing more food and resorting to family planning measures but also by saving the produce from deterioration and wastage, and its efficient allocation to meet the growing requirement of population. In countries with varied climatic conditions, it is a steady struggle of man with insects, mites, rodents and microbes in getting the food stuff.

Need and Objectives of Storage

Since times immemorial, man adopted a particular system of storage and certain produce storage standards. Some of the storage methods are still in use in several regions, in the capacity varying from one kg to several hundred quintals, like hollowed gourds, skin bags, mud bins, excavated stones, woven baskets, underground pits etc., particularly in the tropics. The problems of storage in developing countries are more acute than that in well developed countries and a considerable disparity seems to be there in food availability. All need a food supply that should be nutritious, clean; free from insects, rodents, birds, other animal filth and harmful chemical residues. There are two types of needs, one immediately after harvest and the other in off season. There is no need of storing the produce for immediate requirement but for the latter case, the need to conserve produce properly is inevitable. Further, the need for storage has always arisen for one reason to distribute commodity in deficit production areas and the other based on improved quality standards. In all the way, therefore; growers, processors, transporters and warehouse owner must assist to comply with these requirements.

Vegetable breeding is the purposeful genetic manipulation of qualities in vegetable crops to create new varieties with a set of desired characteristics that are better suited for cultivation, give better yield, quality and are resistant to biotic and abiotic stresses. Vegetable breeding can be accomplished through many different techniques ranging from plant introduction, simply selecting plants with desirable characteristics, to more complex molecular techniques. Classical plant breeding includes hybridization followed by selection and mutation to develop the desirable plant types. With the advancements in genetics, molecular biology and in vitro culture, different molecular genetics tools are also being used in vegetable breeding.
Sexually propagated vegetable crops
1. Plant Introduction
The term “Plant introduction” describes the movement of crop plants, species, varieties, wild species, etc. from their original growing sites to other locations where it was not grown earlier. Plant introduction is essentially the method of cultivating crop plants in a new environments. The agro-climatic conditions can differ greatly depending on the origin of the plant species and the area where they are introduced. Plant introduction is a time-honored strategy for improving crop diversity.

1. Forecasting Rain and Frost

i. Behavior of Ants and Goats:

• Ants: When ants come out of their nest carrying their pupae, it indicates that rain will come soon.

• Goats: Goats can sense approaching rain and signal it 2 to 3 days in advance through behavioral changes. They change their usual resting places, show little interest in taking food or water, and bawl and shout throughout the day.

2. Tree Fruiting Patterns:

• Neem Tree (Azadirachta indica): If the neem tree bears plenty of fruit.

• Baval Tree (Acacia nilotica): If the baval tree produces plenty of pods.

• These observations suggest that the total rainfall for the monsoon will be high.

Weed control methods may be classified into two groups as follows:

    1.    Preventive method
    2.    Curative or remedial measures. Curative measures are sub-divided into eradication and control.

(a) Preventive Methods

Prevention is the measures taken to prevent the introduction or spread  of the weeds to new areas and to check the infestation prior to the germination of weed seeds. To achieve this, a wise step to be followed the principle- “Prevention is better than cure”.

Skeleton Leaves

Skeleton leaves are semi-transparent leaves, also called fossil leaves. They are prepared by soaking the fresh leaves in bleached water and then by hand rubbing the soft green flesh from the network of veins. The leaves can be then dyed to variant colors to give them a beautiful and pleasing appearance. Although delicate by nature, they are actually sturdy and can easily be glued to a variety of surfaces. They come in several colors and sizes to meet a complete line of crafting needs. The delicate beauty of these leaves is sometimes meant to capture life, hope and memories. These delicate, gorgeous skeleton leaves are a perfect accent for all craft projects. They make a beautiful and elegant statement when they adorn your wedding invitations. They work surprisingly well on gift tags, greeting cards, scrapbooks, collages, papermaking, stenciling, and stamping. They are perfect for decorating bridal bouquet, wedding reception table, candles, and wedding favors.

Introduction

Impact assessment is the process of identifying the future consequences of a current or proposed action. It is used to ensure that projects, programmes and policies are economically viable, socially equitable and environmentally sustainable. Programme Monitoring and Evaluation (PME) efforts are critical issues in management of agricultural research and development (R & D). In times of fiscal constraint, more emphasis is placed on accountability and programme effectiveness. As a management tool, PME is an integrated approach that incorporates the viewpoints of clientele, policy-makers, researchers and donors to address the intended goals. Public and private support to research will depend largely on efficiency issues and ability to reckon with multiple objectives, ranging from food security to sustainability, poverty alleviation, gender issues, export, etc. The PME of agricultural R & D projects is critical when the intended R&D has multiple outcomes. Monitoring and evaluation (M&E) of development activities provides government officials, development managers, and civil society with better means for learning from past experience, improving service delivery, planning and allocating resources, and demonstrating results as part of accountability to key stakeholders (World Bank, 2004).

To comprehensively analyse the perceived effects of contract farming on employment generation and poverty alleviation, it is imperative to adopt a systematic and scientific approach. This chapter elaborates on the research design, methodology and tools employed in conducting the present study. It encompasses the rationale behind the study locale selection, sampling techniques, instrument construction, data collection, variable operationalization and analytical methods. These methodological decisions are aimed at unearthing empirical evidence and formulating theoretical insights into the socio-economic implications of contract farming.
The study primarily seeks to understand the perceived outcomes of contract farming on rural livelihoods, particularly its influence on employment and poverty. Furthermore, it explores associated constraints and socio-economic characteristics of contract farmers, thereby constructing a robust conceptual framework grounded in empirical observations. The chapter is structured under the following sub-sections:
a) Locale of research
b) Sampling design
c) Pilot study
d) Hypothesis
e) Variables and their measurement
f) Method of data collection
g) Statistical and analytical tools

Methylene-cyclo-propyl-glycine (MCPG), is a cyclopropyl derivative, grouped into the amino acids that have been isolated from the higher plants (Fowden, 1960). MCPG was identified to be closely related to two other cyclopropyl derivatives (Fig. 1), 1-aminocyclopropane – 1 – carboxylic acid from Vacciniumvitis-idaea and perry pears (Vahatalo and Viertanen, 1957; Burroughs, 1957) and â- methylenecyclopropyl alanine (MCPA or Hypoglycin A) from Blighia sapida (Hassall and Rayle, 1955; Anderson et al., 1958; Ellington, Hassall and Plimmer, 1958).

ABSTRACT

Methylotrophy is a phenomenon by which microorganisms derive energy, and in many cases, cell carbon from reduced molecules that have no C–C bond (also called C1compounds). This gains significance because of its unique mode of nutrition, the ability to utilize more than one metabolic pathway and its vast distribution among various groups of bacteria and yeasts. It is one of the earliest modes of energy metabolism present in microorganisms, through which microorganisms are able to utilize a wide range of compounds, which are considered unsuitable for most forms of life. In the present chapter we focus on various aspects of methylotrophy, and the application of methylotrophic bacteria in a spectrum of biological activities and commercial possibilities.

Metritis is a condition seen in cattle in which there is the inflammation of the entire thickness of uterine wall (endometrium, myometrium and serosa). When it occurs just after parturition, it is called puerperal metritis. It usually occurs after abnormal birth like in case of dystocia, abortion, RFM etc. The animal shows systemic signs like increase in temperature initially, increase in respiration rate, anorexia, toxemia etc. There is expulsion of large volume of foul smelling reddish to brown discharge from vagina frequently associated with straining. The expelled discharge from vagina is often with pieces of degenerating placenta.

1.    The word “economics” has been derived from the Greek word_______ which means household management?

(a) OIKONOMICAS

(b) OIKOS

(c) NOMOS

(d) OICONOMICAS

2.    The term “microeconomics” has been derived from a Greek word ____________.

(a) MICROS

(b) MIKROS

(c) MACROS

(d) MACROS

10.1 Introduction

As one ofthe oldest and most widely used renewable energy sources, hydropower generates electricity by using the natural flow of flowing water. Streams and rivers have been used for producing mechanical energy for thousands of years. Hydroelectric power plants are located near or on water sources since they derive their power from flowing water. The amount of energy that can be released in moving water depends on the volume of the water flowing and the elevation change from one point to another, often called head. Generally, hydropower plants can produce more electricity when there is more water flow and a higher head. 

As the name indicates, micro-organisms are very minute, cellular, living organisms which cannot be seen with the naked eyes (however, large colonies of fungi can be seen). Micro-organisms affecting stored grain can be divided into the following groups in order of their significance:

1. Fungi
2. Actinomycetes
3. Yeast
4. Bacteria
5. Protozoa (not found in sound food grains)

Fungi, bacteria and actinomycetes are grouped under the Thallophyta of the plant kingdom. The most important factors for their normal development are: (i) Nature of Food, (ii) Moisture, (iii) Oxygen and (iv) Temperature.

Micro propagation in also called as in vitro propagation. In vitro is a Latin word, meaning ‘in glass’. It refers to procedures done in controlled artificial environment, outside a living organism. These procedures naturally occur in the living system. Opposite to in vitro is in vivo which is also a Latin word, meaning ‘in living’. Biological processes that occur within the living system.

Micro propagation refers to the multiplication of plants, in aseptic conditions in containers under controlled nutritional and environmental condition. Micropropagation is done in artificial growth medium from various plant parts like meristem tip, embryos, anthers, axillary buds, leaf section, shootlips etc. It is a method by which a true to type and disease free plant can be regenerated from a miniature piece of plant throughout the year. Some popularly grown crops from tissue culture are as follows

Ornamental plants - orchids, lilium, gerbera, cordyline, begonia, ficus, etc.

Micropropagation refers to the production of plants from very small plant parts, tissues or cells, grown aseptically in a test tube or containers under controlled nutritional, environmental and aseptic conditions. It is also called as in vitro propagation, which implies clonal, true-to-type propagation of plants by a variety of tissue and cell culture methods and is considered as the most efficient and commercial method of plant propagation. Tissue culture or in vitro culture is two broadly used terms for micropropagation, which basically include aseptic culture of various plant parts. Micropropagation was developed as the result of accidental findings of French Professor G. Morel in 1958, when he was attempting to obtain virus-free plants through meristem culture in dahlia and orchids. All the biological principles of micropropagation technique are based on the phenomenon of totipotency of cell, in which a cell of a plant has the capacity to regenerate in to a full-fledged plant having different organs. German plant physiologist, Haberlandt (1902) is known as the father of tissue culture technique, who for the first time coined the term totipotency.

In genetics, microRNAs (miRNA) are single-stranded RNA molecules of 21-23 nucleotides in length, which regulate gene expression. miRNAs are encoded by genes from whose DNA they are transcribed but miRNAs are not translated into protein (i.e. they are non-coding RNAs); instead each primary transcript (a pri-miRNA) is processed into a short stem-loop structure called a pre-miRNA and finally into a functional miRNA. Mature miRNA molecules are partially complementary to one or more messenger RNA (mRNA) molecules, and their main function is to down-regulate gene expression. They were first described in 1993 by Lee and colleagues in the Victor Ambros lab .

MicroRNAs can be encoded by independent genes, but also be processed (via the enzyme Dicer) from a variety of different RNA species, including introns, 3' UTRs of mRNAs, long noncoding RNAs, snoRNAs and transposons.

The genes encoding miRNAs are much longer than the processed mature miRNA molecule; miRNAs are first transcribed as primary transcripts or pri- miRNA with a cap and poly-A tail and processed to short, 70-nucleotide stem-loop structures known as pre-miRNA in the cell nucleus. These pre- miRNAs are then processed to mature miRNAs in the cytoplasm by interaction with the endonuclease Dicer, which also initiates the formation of the RNA-induced silencing complex (RISC). This complex is responsible for the gene silencing observed due to miRNA expression and RNA interference. The pathway in plants varies slightly due to their lack of Drosha homologs; instead, Dicer homologs alone effect several processing steps. The pathway is also different for miRNAs derived from intronic stem-loops; these are processed by Dicer but not by Drosha. Either the sense strand or antisense strand of DNA can function as templates to give rise to miRNA.

This practical assignment is conceptualized and designed to improve skill of the students on micro teaching for managing teaching situation.

Purpose

To get students acquainted with the micro teaching exercise.

To allow the students for progressive training for the management of teaching situation.

To improve specific micro-teaching skills involved in teaching.

Introduction

Home to the largest population of poor in the world, India has been a natural candidate for experimenting with micro finance as a tool for poverty alleviation. With a nationalized formal banking sector that has emphasized rural and developmental banking for several decades now, India’s involvement with small credit targeted primarily at the rural poor is hardly new. Micro-credit, in the sense of small loans to the poor, is of ancient origin in India. Micro-Finance has, in recent times, come to be recognised and accepted as one of the new development paradigms for alleviating poverty through social and economic empowerment of the poor, with focus on empowering women. Experiences of different anti-poverty and other welfare programmes within the country and elsewhere, have shown that the key to its success lies in the participation of community based organisations at the grassroot level. People’s participation in credit delivery, recovery and linking of formal credit institutions to borrowers through the intermediation of Self Help Groups (SHGs) have been recognised as a supplementary mechanism for providing credit support to the rural poor. Poor people need micro-credit for various and different purposes. It may be to meet the major household expenses, emergency needs or even basic livelihood support.

Skills and knowledge are the driving forces of economic growth and social development of any country. India is moving progressively towards becoming a ‘Knowledge economy’ it becomes increasingly important that the focus is made on advancement of skills and these skills have to be relevant to the emerging economic environment. In old economy, skill development largely meant development of shop floor or manual skills. In new or knowledge economy the skill sets can range from professional, conceptual, managerial, operational behavioral to interpersonal skills and inter-domain skills. In the 21^st century as science progresses towards a better understanding of the miniscule, that is, genes, nano-particles, bits and bytes and neurons, knowledge domains and skill domains also multiply and become more and more complex. Micro enterprise development is one of the strategies seen to bring about pro-poor economic growth.

SUMMARY

Agriculture is the largest user of fresh water for irrigation to fulfill the increasing demand of food, feed, fiber, fuel and other need of ever-increasing population. Water availability to agriculture is characterized by declining in share with time, over exploitation, improper mismanagement, deterioration in quality as well as environment. This could be addressed using sustainable management of irrigation water. As per the Brundtland Commission report of United Nations, sustainable development is the development that meets the needs of the present without compromising the ability of future generations to meet their own needs. Sustainable water management aims to improve water productivity, availability and quality over an area.  Improved and efficient irrigation techniques and practices enhance water application efficiencies, and thereby reduction in associated damage to environment, which include water logging, soil salinity, runoff and leaching of nutrients and other chemicals polluting water sources, and over exploitation of ground water. Micro irrigation systems have been characterized to be the most efficient irrigation techniques. These have efficiencies even more than 90%, doubled water productivity with improved quality of produce, reduced runoff and leaching of nutrients and chemicals to water sources. Application of fertilizer through drip irrigation called fertigation could be adopted for efficient use and saving of fertilizer.  Fertilizer savings of 20-60% and 8-41% increase in yields of horticulture and vegetable crops has been realized through fertigation.

Introduction

Water is the driver of life/ liquid of lift or elixir of life for flora and fauna. It plays an important role in plant as it constitutes about 35-95% part of plant. Water is a mode of transportation of nutrients from roots to shoots and vice-versa, a thermal regulator of temperature inside the plants, source of hydrogen for the reaction of CO₂ for photosynthesis and is essential for maintaining turgidity and servility of plants. Water is required for agriculture as well as for other sectors (domestic, industries, etc) and the demand of water is increasing alarmingly.  Presently agriculture consumes over 80 per cent of total water consumption in India. The country is endowed with many perennial and seasonal rivers. The river, low ever 71 per cent of water resources is concentrated in 36 % of the geographical at area. Water resource of India, population in per capita water ability and estimates of water needs are given below.

Microalgae have long been recognized as good potential sources for biofuel production due to their relatively high oil content and rapid biomass production (Schenk et al., 2008; Hu et al., 2008). These photosynthetic organisms thrive in a variety of aquatic habitats, including lakes, rivers, oceans, and even sewage, demonstrating their adaptability to diverse environmental conditions. They can tolerate a wide range of temperatures, salinities, and pH levels, as well as varying light intensities and aquatic or desert conditions. Additionally, microalgae can grow independently or in symbiosis with other organisms, making them highly versatile for various cultivation systems (Mutanda et al., 2011). Algae are generally grouped into three major categories: Rhodophyta (red algae), Phaeophyta (brown algae), and Chlorophyta (green algae), and are further classified by size into macroalgae and microalgae. Macroalgae are large, multicellular organisms visible to the naked eye, while microalgae consist of microscopic single cells that can be either prokaryotic, similar to cyanobacteria, or eukaryotic, similar to green algae (Fields et al., 2014; Maltsev & Maltseva, 2021). Microalgae are capable of producing a wide array of valuable bioproducts, including polysaccharides, lipids, pigments, proteins, vitamins, bioactive compounds, and antioxidants. They grow at a much faster rate compared to terrestrial crops and can be cultivated on non-agricultural land using non-potable saltwater or wastewater, reducing competition with food crops and ensuring sustainability (Lee et al., 2014). This has made microalgae an increasingly attractive alternative feedstock for biodiesel production, which relies heavily on the high lipid content of microalgae. The majority of these lipids are stored in the form of triacylglycerols, a type of oil that is ideal for biodiesel production. Beyond biodiesel, microalgae can also be utilized in various other energy production pathways. Some microalgal strains can produce hydrogen gas under specific growth conditions, while algal biomass can be combusted like wood or digested anaerobically to produce methane biogas, which can be used for heat and power generation. Additionally, algal biomass can be processed into crude bio-oil through pyrolysis, further expanding its applications as a renewable energy source (Mumtaz et al., 2019).

Nowadays, revolutionary changes in entire globe have led to the economic, cultural and scientific development in our society and have augmented considerable changes in our food habits and life-style. Continuously growing population had noticeably increased the pressure on land, water and energy resources. All these resources are scarce and going to be exhausted in coming decades at the present rate of consumption. Undoubtedly, production of food is more imperative than the production of energy or any other bioproducts. Moreover, with the world population rising to 9 Billion in 2050, with many inspiring to a western life style and diet, this challenge should be addressed urgently (Draismaa et al., 2013). The challenge to produce about 70% more food by 2050 for a growing and increasingly affluent world population has motivated several review papers addressing the different dimensions of the food system and food security (Verburg et al., 2013). Presently, human beings consume approximately 2.5 billion tons of dry weight of harvested crops which include approximately 2.3 billion tons of cereals (e.g., rice, wheat, and corn kernels) and grass from managed pastures (Smil, 2000).

Introduction 
Fossil Fuels like Coal, Coal product, Natural Gas, Petroleum, Crude Oil provide 80% of the world’s energy. However, burning them releases harmful pollutants, including carbon dioxide, nitrogen oxides and the sulfur oxides, contributing to global warming. To combat this, the World is shifting towards cleaner, renewable energy sources called biofuels. Biofuels are made from organic materials like plants and waste, offering several benefits: Renewable and Sustainable, Less greenhouse gas emissions, non-toxic [6]. Common biofuels include are: Biodiesel, Biogas, Bioethanol. These alternative fuels help reduce our reliance on fossil fuels, mitigating climate change and promoting a healthier environment. Biodiesel replaces fossil-based diesel/petrol made through Trans-esterifi cation process. The benefi ts include clean energy, renewable resources and the domestic production. Generations of biodiesel are: First Generation include Edible crops (Soybean, Corn, Sugarcane). Second Generation includes Non-edible biomass(Straw, Hay). Third Generation include Microalgae (70% yield). Fourth Generation include the genetically modifi ed algae and electro-biofuel. [2] Biodiesel production has changed over time. Initially, edible vegetable oils like soybean, corn, sunfl ower and palm oil were used. However this raised concerns: using valuable farmland for fuel instead of food, competing with food production, weather and soil quality affecting the crop yield , fertilizer use releasing harmful nitrous oxide greenhouse gas. To address these issues, focus shifted to non-edible oils and now Microalgae, offering; sustainable, climate-resilient production, no competition with the food crops, reduced greenhouse gas emission, higher oil yield. Microalgae -based biodiesel is the future providing a cleaner, more effi cient alternative.[4]

Introduction 
Fuels are essential to human existence and account for 70% of the world’s energy use. The demand for fossil fuels is rising daily due to the ongoing global population boom and has now reached a crisis point. The world’s current reserves cannot keep up with the projected 40% rise in demand between 2010 and 2040 [1]. According to Energy Information Administration (EIA) statistics, the world’s fossil fuel sources will run out in fewer than 50 years [2]. In addition, burning fossil fuels and living in an industrialised environment have released large amounts of carbon dioxide (CO2), carbon monoxide (CO), sulphur dioxide (SO2), nitrogen oxides (NOx), as well as a significant number of potentially toxic compounds into the atmosphere. This has severely degraded the natural resources that are currently available, including fossil fuels. The human and natural ecology have suffered significant damages as a result of the effects of global warming in recent years [3]. As a result, cutting CO2 emissions is crucial to preventing the negative effects of global warming. Therefore, there has been a greater focus on the improvement of CO2 capture and sequestration systems to significantly lower CO2 emissions from a variety of sources, including vehicle exhaust emissions and industrial flue gas [4] . Fuel production needs to be sustainable in terms of the environment and the economy, meaning it needs to be able to reduce CO2. It is critical to find inexpensive, sustainable, and clean energy options in order to handle the current circumstances. Currently, several traditional renewable energy sources, such as solar, biofuels, hydroelectric, geothermal, or wind are being used with varying degrees of success and detriment. Biofuels are sustainable and renewable energy sources that have emerged as a superior alternative to traditional fossil fuels in energy production. Biofuels have a lot of potential because they are abundant environmentally favourable renewable resources and their production is expanding globally, even though they are still more expensive than fossil fuels [5]. Researchers are actively investigating biomasses as a means of obtaining energy from renewable sources. It is commonly known that through a process called photosynthesis, plants can transform sun energy into chemical forms. Microalgae are fast-growing microorganisms that develop 100 times quicker than terrestrial plants. Their photosynthetic efficiency is also higher (10–50 times greater than terrestrial plants) among biomasses [6]. In less than a day, they can double their biomass. Unicellular microalgae, in contrast to plants, do not divide substantial amounts of biomass into supporting structures like stems or roots, which are costly to create energetically and frequently challenging for harvesting and processing to produce biofuel. Furthermore, they have the ability to adapt to harsh aquatic environments and economically absorb helpful substances. Moreover, 1 kg of microalgae biomass is produced by microalgae cells that fix 1.8 kg of CO2 and have a 50% carbon content [7]. Microalgae can be grown on terrain that is inappropriate for food production because of their high total yield and hence small land use footprint [8]. Microalgal species including Scenedemus sp., Chlorella sp., and Botryococcus sp.; are common, easier to grow than other species and may have higher lipid contents [9].

In the area of pharmaceutical discovery, screening methods continue to play a major role in drug discovery. The screening process is simply testing molecular entities (compounds/natural products) for activity to advance those that exhibit the desired activity. This process has evolved over time in both the testing methods and in the degree of testing. Originally, the screening was done entirely by dosing animals and studying their responses. Then came test tube experiments and now, testing is conducted either with cell lines or with purified proteins in miniaturized test tubes called microplate wells. Screening by microplate has allowed the degree of testing to increase from a very small selected set of compounds to libraries, which contain thousands of test substances. Technological improvements in many areas of science and information technology have led to the evolution of screening methods from time consuming, animal dosing studies to high throughput screening (HTS) methods that have the potential to generate very large datasets of information in a very short time. Today, developments in bioinformatics have opened up the possibility of virtual (in silico) screens for targets where there is sufficient knowledge of the target or of its ligand. High throughput screening has become a pivotal part of drug discovery for identifying potential lead molecules for specific disease targets. The success of screening is measured by several factors including the throughput and costs of the assays, but ultimately by how many of the HTS hits progress forward through the drug discovery pipeline. Miniaturization of assays by conversion from 96- to 384- to 1536- wells to well-less platforms, such as microarrays and the utilization of automated equipments are methodologies implemented to increase the throughput and reduce the cost of the screen.

Soil is the outer loose layer of earth consisting of layers of mineral material (regolith) whose properties differ from one another and these differences are largely due to the variation in biological activities. Soil micro-environment principally comprizes of inorganic particulates (sand, silt and clay) plus certain oxides and hydroxides, plant roots, organic matter, microbial biomass and aqueous and gaseous phases. The uneven distribution of these components provides a great variety of conditions from field to micropores. The interaction of physical and chemical factors contributes to the creation of varied soil habitat and hence determines the composition and activity of soil biota at a particular site and time. Over geological times, microorganisms have had great opportunity to become distributed worldwide. Such growth and development of microorganisms as well as species diversity is of considerable significance. The existing geographic distribution is reflective of long term adaptation of microorganisms to the various environmental conditions. The preferred environment of an organism is usually referred as ‘habitat’.

1.    Introduction    360
2.    Feedstocks for Biodiesel Production    361

    2.1.    Fats and oils, 362
    2.2.    Alcohols, 363
    2.3.    Catalysts and neutralizers, 367

1.    Introduction    468

2.    Biofuel Cell    470

    2.1.    Microbial-based Biofuel Cells, 471
        2.1.1.    Microbial bioreactors producing H2 for conventional fuel  cells, 475
        2.1.2.    Microbial fuel cells producing electrochemically active metabolites in  
                the anodic compartment, 476
        2.1.3.    Microbial fuel cells operating in the presence of artificial electron relays, 477
        2.1.4.    Microbial fuel cells producing electricity during wastewater treatment, 483
        2.1.5.    Microbial fuel cell producing  electricity from acetate or butyrate, 484

1.    Introduction    302
2.    Raw Materials for Alcohol Production    304

    2.1.    Sugary substrates, 306
    2.2.    Starchy residues (cereal grains), 309
    2.3.    Cellulosic materials (crop residues), 314

3.    Microorganisms for Alcohol Production    316

    3.1.    Comparision of ethanol production by bacteria and yeasts, 318