eChapters

Who discovered bacteria?

a) Leeuwen hock b) Ledge berg

c) Walkman and Zinder d) None

The fungi are usually:

a) Parasites or saprophytes b) Lithophytes

c) Epiphytes d) Insectivorous

According to Pasteur statements which one of the following is true

a) Living organisms discriminate between stereo isomers

b) Fermentation

c) Living organisms doesnít discriminate

d) Both a and b

The method to preserve foods in sealed glass bottles by heat in boiling water was developed by one among the following scientist?

a) Edward Jenner (1798) b) Francois Nicolas Appert (1804)

c) Lazzaro spallanzani (1765) d) None of the above

Nisin is produced by strains of

a) Streptococcus lactis b) Pseudomonas

c) E. coli d) Clostridium

The term nanotechnology, buzzword of present day science owes its origin from the Greek word ‘nano’ literally meaning dwarf. When it is expressed in terms of dimension one nanometer equals to one billionth of a meter (1nm = 10-9 m). A nanometer is a thousandth of a thousandth of a thousandth of a meter, about 60,000 times smaller than a human hair in diameter or the size of a virus, a typical sheet of paper is about 100,000 nm thick, a red blood cell is about 2,000 to 5,000 nm in size, and the diameter of DNA is in the range of 2.5 nm. Therefore, nanotechnology deals with matter that ranges from one-half the diameter of DNA up to 1/20 the size of a red blood cell. 

It all started after the famous lecture, “There is plenty of room at the bottom” given by Richard Feynman on December 29, 1959. Nanomaterials are intermediate between macroscopic solid and of atomic and molecular systems. Nanomaterials have certain properties which make them different from that of the bulk materials, including large fraction of surface atoms, high surface energy, spatial confinement, and reduced imperfections. Nanotechnology deals with various structures of matter having dimensions of the order of a billionth of a meter (Ray Unmesha. 2018). From the advent of nanotechnology, people realized that certain materials can exhibit different properties based on its size and shape.
 

Biological diagnostic methods rely on the transmission and amplification of the pathogen in indicator hosts that develop symptoms. These methods test the biological properties of pathogens and early successes in the control of intracellular pathogens were largely due to the diagnosis of the causal agents by biological tests. The sensitivity of biological/serological tests is usually determined by the minimum number of pathogen propagules required to initiate infection. Inoculation with fewer particles could lead to false negative results. Biological techniques for disease diagnosis and pathogen detection are usually highly accurate but too slow and not amenable to large-scale application. 

Agri-nanotechnology is a new branch of biology that has originated due to the compatibility of nanosized inorganic and organic particles with biological functions. Based on enhanced effectiveness, the new age drugs are nanoparticles of polymers, metals, or ceramics, which can facilitate several biological applications. Plant diseases are caused by pathogens including fungi, bacteria, mycoplasma, viruses and viroids, nematodes, parasitic plants, and protozoa. 

With the development of improved systems for monitoring environmental conditions and delivering pesticides appropriately, nanotechnology can further improve our understanding of the biology of different crops and thus potentially enhance yields. It can offer routes to plant disease diagnostics, insect-pest management and efficient pesticides utilization. Nanostructure catalysts will help in increasing the efficiency of pesticides allowing lower doses to be used. 

Biocompatible, biodegradable, intelligent and responsive materials are currently an emerging area of interest in the field of efficient, safe, and green pesticide formulation. Using nanotechnology to design and prepare targeted pesticides with environmentally responsive controlled release via compound and chemical modifications has also shown great potential in creating novel formulations. Special attention was paid to intelligent pesticides with precise controlled release modes that can respond to micro-ecological environment changes such as light-sensitivity, thermo-sensitivity, humidity sensitivity, soil pH and enzyme activity (Huang et al., 2018).

Nanoscience and nanotechnologies are set to transform the global industrial landscape, but the debate on how to regulate environmental, health and safety risks is lagging behind technological innovation. Current regulatory efforts are primarily focused on the national and regional level, while the international dimensions of nanotechnology governance are still poorly understood and rarely feature on the international agenda. With the ongoing globalization of nanosciences and the rapid expansion of international trade in nanomaterials, however, demand for international coordination and harmonization of regulatory approaches is set to increase. However, uncertainty about nanotechnology risk poses a profound dilemma for regulators and policy-makers. Uncertainty both creates demand for and stands in the way of greater international cooperation and harmonization of regulatory approaches. Falkner Robert and Jaspers Nico (2012) reviewed the emerging debate on nanotechnology risk and regulatory approaches, investigates the current state of international cooperation and outlines the critical contribution that a global governance approach can make to the safe development of nanotechnologies. 

Nanotoxicity is the study of the toxicity of nanomaterials that focuses on how the size and unique properties of nanoparticles influences their impact on plant helath, human health and the environment. Plants are essential fundamental components of all ecosystems and the interaction between nanoparticles (NPs) and plants is an indispensable aspect of the risk assessment. NP phytotoxicity, an important precondition to promote the application of nanotechnology and to avoid the potential ecological risks was reviewed (Jie Yang et al., 2017). 

Nanotechnologies are being spoken of as the driving force behind the new industrial revolution. The mass production of these nano materials and their applications mean a dramatic increase of workers dealing with Engineered Nano Materials (ENM) and becoming possibly exposed to resulting hazardous effects and increased environmental burden of the environment due to the leaks of these materials from industrial processes. It is, hence, essential to consider the importance of ENM safety and their new applications and understand that it is important for these materials and products to be safe to enable successful promotion of nanotechnology for useful applications (Eman M Osman, 2019). 

1.1. Background Introduction

We live in a world with “throw-away” societies that are involved in a pattern of consuming packaged products that don’t even last past a single use or even a year and discarding the waste. This lifestyle generating waste is negatively impacting public health, environment and imposing socio-economic problems on us. The waste is continuously consuming our land, resources, and energy for storage, management, treatment, and disposal. Solid waste was an urban issue initially but has reached every corner nowadays. 

1.1 Introduction

Increased emission of greenhouse gases results in climate change which becomes one of the most prominent environmental issues to the world today. Carbon dioxide (CO2) is the major greenhouse gas whereas nitrous oxide (N2O) and methane (CH4) are other substantial contributors to climate change (IPCC, 2014, Tongwane et al., 2016). However, these gases are emitted in minimal quantities in comparison to carbon dioxide but impart more global warming effect by absorbing more infrared radiations (Oertel et al., 2016). The warming potential of one kg of CH4 is 23 times more than one kg of CO2 while  in the case of N2O, it was nearly 300 times more than CO2 (Ramaswamy et al., 2001, Bharti et al., 2019; Biswas et al., 2010). Around one-third of methane and two –thirds of nitrous oxide come from soil (Prather et al., 1995, Tubiello et al., 2015, Smith, 2016).

3.1 Introduction

A Massive increase in population, urbanization and industrialization have led to an increase in the quantity and composition of waste. The waste is increasing day by day and is estimated to reach up to 2.2 billion tonnes by 2025, according to the reports by World Bank. It becomes a necessity for every nation to fore look upon the waste management system for their respective countries. From the collection, segregation, transportation, disposal all have become an important criterion to deal with. Among the available disposal options; the easiest and environment friendly is “Recycling”. 

4.1. Introduction

Presently, in the world, there are 195 countries according to the United Nations, and the number would even go up to 247 if we consider the disputed territories like Isle of Man, Hong Kong, etc. (United Nations, 2016) (Kershner, 2020). But the majority of the countries are still struggling to provide basic life-supporting amenities like nutrition, shelter, and employment. Conjointly, termed as emerging and developing countries such as India, Vietnam, Venezuela, Utopia, etc. (Ahmed and Shimada, 2019). Most of the developing countries are situated mainly in the continent of Africa, Asia and South America. They have got independence for the European countries like England, France, Portugal and Spain in the mid and late 20th century. 

Definitions: Before starting the chapter, it is better to understand the basic definitions of the terms commonly used in this chapter.

Waste: Waste is any material or object which is discarded after its primary use by its holder. A holder means the person who produces and keeps the waste. 

6.1 Introduction

Urbanization is now a global phenomenon, but in developing countries the implications are more pronounced. Natural growth of population, reclassifications of habitation and migration trends are important in urban population in India. The population of India, which accounts for 27 per cent of the total population, was 285 million according to 2001 census. International experience shows that the pace of urban development accelerates when a country’s urban population crosses close to 25 percent of the total population (like in India).(Kumar and Chakrabarti, 2010). 

7.1. Developing Sector in Scientific and Technological Approach of Waste Management

From the very past, many traditional practices have been put into use for waste management like landfilling, composting, and recycling, which are practiced even today. However, the boundless upsurging waste compels the modern world to modify the traditional methods or to introduce innovative techniques with the grace of technology

8. Introduction

The idea of sustainable development stands on the concept of maintaining the best possible environment within the availability of minimal resources and for the convenience of the society. Now-a-day the term “sustainability” gained a lot of attention due to challenges and problems faced in tackling the current situation of global pollution. One of the vital areas of research needs to be addressed in concern with sustainable development is the new innovations to enhance sustainability (Silvestre and Silva, 2014). 

Introduction

With ever-increasing population growth, waste management has become a major challenge threatening environmental sustainability. The total solid waste generation was recorded to be 1.3 billion tonnes per year by the world’s cities in 2012, with an ecological footprint of 1.2 kilograms per person/day. With the intensity with which the population growth is happening coupled with urbanization, predictions are made that by the end of the year 2025, 2.2 billion tonnes of municipal waste will be generated (The World Bank, 2018). 

10.1 Introduction

The consistent process of rapid urbanization and expeditious developmental activities have severely impacted the environmental components and thereby the quality of life. With limited natural resources, individuals and societies have to learn to bring in attitudinal changes in order to accomplish environment sustainability (UNESCO). From the Book “Our Common Future” (Brundtland Report), the concept of Sustainable Development can be understood as the “development that meets the needs of the present, but does not threaten the ability of future generations to meet their own needs”. 

ECONOMICS is the term derived from a Greek word ‘OIKOS’ means a HOUSE and NAMEN means TO MANAGE, i.e. to manage a household using limited funds available in most possible manners. The founders of Economics defined it as-‘The Science of Wealth’. In general, we may say that Economics is the social science that studies economic activities. Economists at different times have emphasized different aspects of Economic activities, and have arrived at different definitions of Economics.

MARKET & MARKETING: CONCEPT OF MARKET AND CLASSIFICATION OF MARKETS

Meaning of Market: The Term Market is derived from Latin word ‘Markatus’ means Merchandise, Wars, the Traffic, Trades or Place where business is conducted. The Market means a place, as an open space (in the village) or a larger building, where actual buying and selling takes place. The market is an assembly or a meeting of people for their private purchase and sale of goods at a stated time and place. Example-Village Fairs or periodical markets.

MEANING & DEFINITION OF ACCOUNTING

Accounting is the science of recording, classifying and summarizing business transactions and events primarily of a financial character in a significant manner and interpreting and communicating the results to decision-making persons thereof.

The AICPA defined, Accounting is the process that are used in accounting, recording, classifying, summarizing and interpreting the monetary language in which financial language is expressed.

BOOK KEEPING & GENERAL ENTRY SYSTEM

Business Transactions can be recorded in any system, which provides the necessary financial position of a business and results of transactions. The Business transactions are recorded through systems of book keeping as maintained in accounting are as follows:

Book Keeping: This is an activity concerned with the recording of financial data relating to business operations in a significant and orderly manner. Book keeping is the record making phase of accounting. Accounting is based on a careful and an efficient book keeping system.

The essential idea behind maintaining book keeping records is to show correct position regarding each head of income and expenditure. It is concerned with the recording of business transaction in a significant and orderly manner which is mechanical and repetitive in character. 

DEFINITION OF ENTREPRENEUR & ENTREPRENEURSHIP

Entrepreneurs: The word ‘Entrepreneur’ is derived from the French verb ‘ENTREPREDRE’, which means ‘to undertake’. The term is used to refer to anyone who undertakes the organizarion and management of an enterprise with independence and risk as well as the opportunity for profit. An Entrepreneur is a person who has a possession of any enterprise or venture and accepts significant accountability for the inherent risk and outcome.

Definitions of Entrepreneurs by Several Social Scientists are as follow

According to J.B. Say: An Entrepreneur is an economic agent, who unites all means of production, such as- Land, labour and capital to produce products.

INTRODUCTION TO FINANCIAL MANAGEMENT

CONCEPT AND DEFINITION OF FINANCIAL MANAGEMENT: Financial management is an integral part of general overall management. The term ‘Financial management’ is managerial activity that is concerned with planning and controlling of financial resources of a firm. Financial management is concerned with managerial decisions that result in the acquisition and financing of long and short-term credits of the farm or any enterprise. Financial management is concerned with the efficient use of all important economic resources that is capital funds of a firm (Solomon Ezra)

Financial management is an application of general managerial principles to the era of financial decision-making. (Howard &Uptron).

Financial management is an area of financial decisions making, harmonizing individual motives and enterprise goals.(Weston & Brigham).

Financial management is an operational activity of a business that is responsible for obtaining and effective utilizing the funds necessary for efficient operations.(Joseph & Massie)

PROJECT AND PROJECT APPRAISAL PROJECT

Project is a scientifically evolves work plan to achieve specific objectives within a specified time limit by consuming planned resources. A Project is a specific plan or design with a specific objective, time, and cost limitations and non-repeated it in nature. UNIDO defines a Project as a proposal for an investment to create and or develop certain facilities in order to increase the production of goods or services in a community over a certain period of time. The Project can be long-term or short-term, limited or comprehensive, sector concentrated on multi-sector concentrated.

PROJECT APPRAISAL or Evaluation is a step-by-step process of collecting recording and organizing information about project results including short-term, immediate output and long-term outcomes from the projects.

CONTEMPORARY ISSUES IN LIVESTOCK ENTERPRISES

GENDER AND ANIMAL HUSBANDRY: The women and men enjoy equal status in the law of the constitution, though there exists a wide difference as looked upon by the society. This gender gap is a socially constructed attribute as being male and females are assigned roles and responsibilities in respect to one’s gender. Gender disparity is a serious concern in all sectors and must be addressed immediately for the development of the country. To address gender issues, it is imperative to understand different fundamental gender concepts to deal with it effectively.

Gender: Gender identifiers social relations between men and women. Gender refers to the relationship between men and women, boys and girls and how this is socially constructed. Gender roles are dynamic and changed over time. Gender determined, what is expected, allowed, and valued in a man or woman in a different context.

Sex: Sex identifies the biological difference between men and women, such as-women can give birth and men provide sperm. Sex roles are universal. These are universal, obvious and generally permanent. It is what, we are born with and does not change over time, differs from place to place.

LIVESTOCK INSURANCE SCHEME

Livestock farming is the main source of livelihood for millions of people in India. Animal husbandry as live resources primarily depends on monsoon-based feeds and fodder production along with the widespread incidence of diseases, droughts floods and fluctuations of market price in livestock products which makes it a risky venture. The natural, social and human risk and uncertainty in livestock farming hamper yield and production as a result of mortality and morbidity of animals to every farmer in the country. This risk is simply future issues that can be mitigated or avoided as a probability issue, whereas uncertainty is the lack of complete certainty with more than one possibility of true outcome or state or result or value as unknown. Individual farmers with limited resources are unable to face such risk and uncertainty of disastrous lost. To mitigate this nature-induced risk, livestock insurance has introduced as an institutional response in the countries in October 1965 as Crop Insurance and in later in 1970 the livestock insurance was started by the government of India.

ESTIMATED DAIRY PROJECT OF 12 CROSS-BREED CATTLE FOR 5 YEARS

Dept. of Veterinary & A.H. Extension Education

West Bengal University of Animal & Fishery Sciences

Mohanpur, Dist-Nadia, West Bengal, India

LIVESTOCK ECONOMICS, MARKETIG, ACCOUNTANCY & ENTREPRENEURSHIP DEVELELOPMENT IN ANIMAL HUSBANDRY & DAIRYING

• A Textbook of Agricultural Extension Management (2019). C. Karthikeyan, R. Sandilkumar & D.Jagannathan. Atlantic Publishers & Distributors, Chennai, Tamil Nadu.
• Accounting Principles for Veterinary and Animal Husbandry Extension (2014). Samaresh Kumar Das, Puspa Publishing House Kolkata.
• Agricultural Extension 1998. Van Den Ban and HS Hawkins. CBS Publishers New Delhi.
• Banerjee, A. and S. Majumdar, D. (2011). Business Economics and Business Environment, ABS Publishing House, Kolkata.
• Entrepreneurship Development and Business Communication 2020. Sagar Mandal, BCKV, Kalyani Publishers New Delhi.

Fungi are very primitive eukaryotic microbe on the earth which are evolved with the earth and its atmosphere. These may be the ancestor of the plants in the process of evolution (Borkar, 2001). The group of ancestral fungi is thought to be represented by the present day chytridiomycota, although the Microsporidia may be an equally ancient sister group. The first major steps in the evolution of higher fungi were the loss of the chytrid flagellum and the development of branching, aseptate fungal filament, which occurred as terrestrial fungi diverged from water molds 600 million to 800 million years ago. The easily recognizable mushroom fungi probably diversified 130 million to 200 million years ago, soon after flowering plants became an important part of the flora and well before the age of dinosaurs. A relatively recent evolutionary radiation perhaps 60 million to 80 million years ago, of anaerobic chytridiomycota occurred as grasses and grazing mammals became abundant, the chytrid fungi serve as symbionts within the rumen of such animals, thereby enabling the grazing mammals to digest grasses.

The fungi are present everywhere. They are in the soil and soil crust, in atmospheric air, in sea and pond water, rivers and water streams, and on the surfaces of almost all the material ,whether living or otherwise, on affected food stuff , vegetables and food grains, on body of human beings, animals, birds and fishes, on plant surfaces, on leathers and clothes in damp weather, on house walls and ceiling in damp conditions, on railway coaches and buses roofs having water leakage and dampness, in caves, on garbage and decomposing material etc. The fungal spores are present everywhere, however the fungal growth occurs where the congenial conditions for their growth is available.

3.1. Plant diseases caused by soil borne fungi

3.1.1. Clubroot Disease causing fungi

Plasmodiophora brassicae (Fig. 125)

It infects Crucifers host species.

Infected plants in the begening shows pale green to yellowish leaves, which later on show wilting in the middle of hot, sunny days, and recovering during the night. Young plants may be killed by the disease soon after infection, whereas older plants may remain alive but become stunted and fail to produce marketable heads.

The most characteristic symptoms of the disease appear on the roots as spindle-like, spherical, knobby, or club-shaped swellings. The swellings may be few and isolated or they may coalesce and cover the entire root system. The older and usually the larger clubbed roots disintegrate before the end of the season because of invasion by bacteria and other fungi.

4.1. Aspergillosis disease causing fungi Aspergillus

Aspergillosis is usually caused by the fungus Aspergillus (Fig. 161) and usually occurs in people with lung diseases or weakened immune system

The different types of aspergillosis can cause different symptoms. The symptoms are similar to asthma symptoms, and include:

4.1.1. Allergic bronchopulmonary aspergillosis (ABPA)

The symptoms of ABPA includes Wheezing, Shortness of breath, Cough and Fever (in rare cases).

4.1.2. Allergic Aspergillus sinusitis

The symptoms of Alleric Aspergillus Sinusitis include stuffiness, Runny nose, Headache and Reduced ability to smell.

5.1 Ringworm (in Cattle) causing fungi

Trichophyton verrucosum

Ringworm is one of the commonest skin diseases in cattle. Ringworm is a transmissible skin infectious disease caused most often by Trichophyton verrucosum, a spore forming fungi. The spores can remain alive for years in a dry environment. Although unsightly, fungal infections cause little permanent damage or economic loss. Direct contact with infected animals is the most common method of spreading the infection.

The symptoms of Ringworm include Lesions with greywhite areas with an ash like surface usually circular in outline and slightly raised that appears on skin. Size of lesions vary and can become very extensive. In calves the infection is most commonly found around eyes, on ears and on back. In adult cattle infection on chest and legs are more common (Fig. 183)

6.1. Aspergillosis disease causing fungi

Aspergillus fumigatus

Aspergillosis is a fungal infection that commonly causes respiratory disease in pet birds. It can infect both upper (nose, sinuses, eye, and trachea) and lower (lung and air sacs-a specialized part of the respiratory tract that birds have) respiratory organs or more broadly distributed systemic infections (Fig. 187).

The Symptoms includes Weight loss, Open-mouth breathing or gasping for breath, Tracheal movement or in-out-in movements in the region of the neck, Tail-bobbing or movement of the bird’s tail up and down while breathing in and out, heavy breathing after flight or exercise, Change in voice and persistent itching or rubbing of cerenares.

7.1. Black Gill Disease (in Shrimps) causing fungi

Fusarium spp

The affected prawns show brown to black spots on the gills. In acute cases gills may completely become brown or black with atrophy and necrosis (Fig. 192).

7.2. Saprolegniasis disease causing fungi

Saprolegnia

Saprolegniasis is a fungal disease of fish and fish eggs, most commonly caused by the Saprolegnia species called “water molds.” They are common in fresh or brackish water. Saprolegnia can grow at temperatures ranging from 0° to 35°C but seem to prefer temperatures of 15° to 30°C. The disease fungus attack an existing injury on the fish and spread to healthy tissue. Poor water quality (for example, water with low circulation, low dissolved oxygen, or high ammonia) and high organic loads, including the presence of dead eggs, are often associated with Saprolegnia infections.

8.1. Fungi useful in food processing

8.1.1. Aspergillus sojae and Aspergillus oryzae

Aspergillus sojae and Aspergillus oryzae (Fig. 198) is a mold species in the genus Aspergillus. Its use was originated in China in the 2nd century AD and spread throughout East Asia where it is used in cooking and as a condiment.

Uses

• It is used to prepare Soy sauce (also called soya sauce) a condiment made from a fermented paste of boiled soybeans, roasted grain and brine,
• In Japan it is used to make the ferment (K?ji) of soy sauce, the mirin and other lacto-fermented condiments like tsukemono. Soy sauce is a condiment produced by fermenting soybeans with Aspergillus sojae, along with water and salt.

9.1. Fungi useful against plant disease pathogen

9.1.1. Trichoderma viride

Trichoderma viride (Fig. 231) fungus is used as a bio-fungicide. It is used for seed and soil treatment for suppression of various diseases caused by fungal pathogens. It is also a pathogen in its own right,causing green mould rot of onion.

The fungicidal activity makes T. viride useful as a biological control agent against plant pathogenic fungi. It has been shown to provide protection against pathogens like Rhizoctonia, Pythium and Armillaria.

It is found naturally in soil and is effective as a seed dressing bioinoculant in the control of seed and soil-borne diseases including Rhizoctonia solani, Macrophomina phaseolina and Fusarium species. When it is applied to the seed, it colonizes the seed surface and kills not only the pathogens present on the cuticle, but also provides protection against soil-borne pathogens.

10.1. Phosphate Solubilisers

10.1.1. Aspergillus spp.

Several species of Aspergillus (Fig. 249) have been reported to be involved in the solubilization of inorganic phosphates such as A. flavus, A. fumigates, A. awamori. A. niger and A. terreus.

These fungi are able to solubilize inorganic phosphate through the production of acids viz. citric, gluconic, glycolic, oxalic acids and succinic acid.

Aspergillus fumigates isolated from compost has been reported to be potassium releasing fungus.

11.1. Pleurotus ostreatus

Researchers in Mexico have shown that oyster mushrooms can break down/ decompose disposable diapers

11.2. Chaetomium globsum

It is a saprophytic fungus that primarily resides on plants, soil, straw, and dung. They are found in habitats ranging from forest plants to mountain soils across various biomes. C. globosum colonies can also be found indoors and on wooden products

C. globosum assists in cellulose decomposition of plant cells.

12.1. Penicillium chrysogenum

Penicillium chrysogenum (Fig. 255) (previously known as Penicillium notatum), produces the antibiotic penicillin, known as WONDER DRUG.

The antibiotics produced from fungus Penicillium is listed in table.

• Food science deals with the physical, chemical, and biological properties of foods as they relate to stability, cost, quality, processing, safety, nutritive value, wholesomeness, and convenience.
• Food science is a branch of biological science and an interdisciplinary subject involving primarily microbiology, chemistry, biology, and engineering.
• Food chemistry, a major aspect of food science, deals with the composition and properties of food and the chemical changes it undergoes during handling, processing, and storage.
• Food chemistry is intimately related to chemistry, biochemistry, physiological chemistry, botany, zoology, and molecular biology.
• Nonenzymic browning involves reaction of carbonyl compounds, which can arise from existing reducing sugars or from diverse reactions, such as oxidation of ascorbic acid, hydrolysis of starch, or oxidation of lipids.

Some chemical and biochemical reactions that can lead to alteration of food quality or safety

1. Carbohydrates are primarily composed of the elements
   1. Carbon, hydrogen and oxygen
   2. Hydrogen, oxygen and carbon
   3. Oxygen, carbon and hydrogen
   4. None of these
2. Carbohydrates have an empirical formula
   1. (CH₂O)_n
   2. (CH₂O)_n+₁
   3. (CH₂O)_n-₁
   4. (CH₂O)_n/₂

1. The protein is derived from Greek word “Proteins” which means
   1. Principal
   2. Prime
   3. Holding the first place
   4. All of the above
2. The name protein to the group of organic compounds that are utmost important to life is suggested by
   1. Mulder
   2. Berzelius
   3. Kulim
   4. Sanger