eBook Chapters

Introduction

Fungicides are biocidal chemical compounds or biological organisms used to kill or inhibit fungi or fungal spores. An insecticide is a pesticide used against insects. Pyrethroids are widely used insecticides in agriculture as they control pests and diseases effectively in plants. Because of their low biodegradability and high persistence, they are extensively found in environmental samples such as air, water, soil, sediments, food and biological tissues [1-4]. Moreover, these chemicals induce cancer and act as endocrine disrupters in several organisms due to their high degree of toxicity. Although the use of most pyrethroids has been prohibited or limited in developed countries, they are used in many developing and under developed countries for controlling of pests and insecticides [5-8].

Abstract

Agriculture provides food, feed, fiber and many other desired products for survival of human beings in the world. Modern agriculture emphasizes on use of hybrid seeds, high yielding varieties and chemical fertilizer to increase the crop productivity within a short period. Indiscriminate use of synthetic fertilizers has led to environmental pollution and deterioration of soil health, which results in depletion of essential plant nutrients and organic matter in soil. On account of the environmental hazard and soil infertility, biofertilizer concept was developed. Biofertilizer are natural fertilizers including bacteria, fungi and algae alone or in combination that augment availability of nutrients to the plants. However, due to its short shelf life period it may not be a suitable candidate for modern agriculture. Thus, it is the need of the hour to develop an economic and eco-friendly nano-biofertilizer for sustainable agriculture.

Introduction

Food packaging is a critical technology addressing the ever-increasing demands for convenience, freshness, ease, shelf-life, safety and security of food products.
 
The main purpose of food packaging is to protect the food from microbial and chemical contamination, oxygen, water vapor and light. The type of packaging used therefore has an important role in determining the shelf-life of the food. Nanotechnology can be intervened to achieve the purpose of food packaging and it will become one of the most powerful forces for innovation in the food packaging industry.

Introduction

Weeds are unwanted plants in agricultural production causing huge losses in crop production system particularly rainfed agriculture. Of the total estimated losses caused in crop production by pests, diseases and weeds in the world, the weeds alone are responsible for one-third of it. Weeds interfere with crop growth and cause an yield loss to the tune of 10% of crop production which amounts to a total loss of about 20 million MT of food grains, valued at over Rs.10,000 crores, at current prices in India. Besides, losses of similar magnitude are experienced in vegetable, fruit and plantation crops. The losses inflicted on biodiversity, environment and health are considerable and have not been quantified scientifically.

Introduction
 
Maintaining and restoring the quality of air, water and soil is one of the great challenges of our time. Most countries face serious environmental problems, such as the availability of drinking water, the treatment of waste and wastewater, air pollution and the contamination of soil and groundwater. There are two main approaches to these problems: the first is to control pollution at source to minimize or eliminate waste production or harmful emissions; the second is to remedy the pollutants that accumulate in the environment. Considering the enormous scale of soil and groundwater contamination, the complexity of the task seems intractable. In many cases, conventional remediation and treatment technologies have shown only limited effectiveness in reducing the levels of pollutants, especially in soil and water (Rickerby and Morrison, 2007).

In plants, mineral uptake is the process in which minerals enter the cellular material, typically following the same pathway as water. The most normal entrance portal for mineral uptake is through plant roots. Some mineral ions diffuse in-between the cells. In contrast to water, some minerals are actively taken up by plant cells. Mineral nutrient concentration in roots may be 10,000 times more than in surrounding soil. During transport throughout a plant, minerals can exit xylem and enter cells that require them. Mineral ions cross plasma membranes by a chemiosmotic mechanism. Plants absorb minerals in ionic form: nitrate (NO₃^-), phosphate (HPO₄^-) and potassium ions (K+); all have difficulty crossing a charged plasma membrane. It has long been known plants expend energy to actively take up and concentrate mineral ions. Proton pump hydrolyzes ATP to transport H⁺ ions out of cell; this sets up an electrochemical gradient that causes positive ions to flow into cells. Negative ions are carried across the plasma membrane in conjunction with H⁺ ions as H⁺ ions diffuse down their concentration gradient.

Introduction

Nano science is the study of the properties of structures of the size smaller than several hundreds of nano meter (nm). Nanotechnology consists in techniques for designing and manufacturing these structures as well as applications arising from these. The development of nanoscience and nanotechnology is in line with the trend towards miniaturisation. As nanoscience and nanotechnology developed, they make the barriers between traditional scientific and technological disciplines more permeable.

Fundamentals of Nanotechnology in Packaging • Overview of Nanotechnology in Packaging: Nanotechnology in packaging refers to using materials manipulated at an extremely small scale (1-100 nanometers) to boost the performance and capabilities of packaging. Techniques like nanoparticles, nanofibers, and nanocomposites are integrated into packaging materials to improve their physical properties, prolong shelf life, and introduce new functions like antimicrobial features. Mechanisms and Key Materials • Nanoparticles: Small particles added to packaging to enhance strength, f lexibility, and barrier properties. Common types include silver, titanium dioxide, and zinc oxide nanoparticles. • Nanofibers: Made from substances such as chitosan, cellulose, or polymers, these fibers increase strength and are useful in active packaging coatings. • Nanoclays: Dispersed in polymers, these increase resistance to gases and moisture, enhancing barrier performance

Nanotechnology is an emerging of field of science which is being highly exploited in the sphere of medicine where “nano” plays a vital role in delivery of drugs or chemical molecules to the point it is required in précised quantities in right proportions. Nanoscience infuses intelligence to the truck load of chemical constituents that are to be delivered at appropriate locations and cleave from the site after the task is complete. Such process will likely to reduce the cost besides ensuring environmental safety. Nanoscale devices with its unique properties make the agricultural system more smart and effective; such devices are capable of responding to different situations by themselves, thus taking appropriate remedial action with the need of external directions from humans. In short, these devices act as a detectors and if need arises serve as a solution/remedy for the particular issue. These smart delivery systems of chemicals in controlled and targeted manner can be considered synonymous to the proposed nano-drug delivery system in human (Patolsky et al., 2006).

Nano-biofertilizers (NBFs) represent an emerging and sustainable approach to improving soil nutritional security and crop productivity in modern agriculture. By integrating beneficial microorganisms with nanomaterials, nano-biofertilizers overcome several limitations associated with conventional fertilizers and biofertilizers, such as low nutrient use efficiency, nutrient losses through leaching, poor microbial survival, and environmental pollution. This chapter provides a comprehensive overview of the role of nano-biofertilizers in enhancing soil fertility, nutrient availability, plant growth, and stress tolerance. The mechanisms underlying improved nutrient use efficiency, soil physical and chemical properties, plant physiological stability, and resistance to abiotic and biotic stresses are discussed in detail. The application of nano-biofertilizers in conventional and precision agriculture systems, including sensor-based variable rate application, fertigation, and data-driven nutrient management, is highlighted. Additionally, various application methods, environmental benefits, and challenges related to safety, scalability, regulation, and farmer adoption are critically examined. Overall, nano-biofertilizers hold immense potential to revolutionize sustainable agriculture by promoting efficient nutrient management, enhancing crop resilience, and minimizing environmental impacts, thereby contributing to long-term food security under changing climatic conditions.

Modern agriculture faces unprecedented challenges due to climate change, unsustainable farming practices, and declining soil health. In response, nanoparticle-bioinoculants (nano-bioinoculants) have emerged as a promising innovation that integrates nanotechnology with microbial inoculants to enhance plant productivity and sustainability. Conventional agriculture contributes significantly to greenhouse gas emissions, degrades microbial diversity, and compromises ecological balance. Beneficial microbes, known as bioinoculants, play a vital role in improving plant health through nutrient cycling, hormone production, and disease suppression. However, their field performance is often limited by environmental instability and low survival rates. Nanotechnology provides a solution by enabling the encapsulation of bioinoculants within nanoparticles, enhancing their stability, targeted delivery, and effectiveness. These nano-scale carriers exhibit high surface-area-to-volume ratios, improved reactivity, and strong adhesion properties, making them ideal for enhancing microbial colonization and activity in the rhizosphere. Additionally, nanoparticles function as nano-fertilizers and nano-pesticides, supporting both plant nutrition and protection against pathogens. Plant-based nanoparticle synthesis further offers an eco-friendly alternative, minimizing ecological and health risks. Studies demonstrated that nano-bioinoculants improve seed germination, stress resilience, photosynthetic activity, and crop yield across various species. They also mitigate abiotic stresses such as drought, salinity, and heavy metal toxicity while promoting systemic resistance against biotic threats. The synergistic combination of nanoparticles and microbial agents enables multifunctional benefits—supporting soil health, reducing chemical dependency, and enhancing agricultural resilience. While challenges remain in understanding

Global agricultural sector moves toward sustainability and resource efficiency, bioinoculants represent a transformative innovation. Bioinoculants (microbial inoculants) are preparations containing beneficial microorganisms such as bacteria, actinomycetes, fungi, and cyanobacteria that, when applied to seeds, seedlings, or plants, and soil, enhance nutrient availability, promote plant growth, and improve soil fertility. In the context of modern agriculture’s ecological challenges, such as soil degradation, overuse of agrochemicals, and climate variability, microbial inoculants offer a sustainable, cost-effective, and environmentally friendly alternative. However, their effectiveness is often limited by environmental stresses, short shelf life, and inconsistent field performance. Nano-bioinoculants address these limitations through the integration of nanotechnology into microbial formulations. This innovative approach improves microbial viability under field conditions, increases interaction efficiency with plant roots, and enables controlled nutrient release, leading to improved crop performance. Plant growth, soil fertility, and stress resistance are strengthened by inoculation using nanoparticles, nano-based biofertilizers, and biopesticides. Their application is particularly relevant in India, where diverse agro-climatic zones and smallholder farming systems demand low-input, high-efficiency solutions. In India, where the demand for eco-friendly and high-efficiency agri-inputs is

Biopesticides are pesticides that are produced naturally by plants, animals, microbes, and other minerals. These represent less of a harm to humans and the environment than chemical insecticides. Nanotechnology is a rapidly developing scientific topic that has numerous uses in numerous industries, including agriculture. The transport of plant hormones, seed germination, water management, transfer of target genes, nano barcoding, nano sensors, and controlled release of agrichemicals are now being investigated as applications for nanotechnology in agriculture (Worrall et al. 2018).

1. Introduction
It is the need of the hour that disposal of radioactive wastes be taken seriouslyand given appropriate attention. We house around one million gallons of liquidradioactive waste awaiting remediation at U.S. Department of Energy’s HanfordReservation alone (Parshley & McDermott, 2021). Not only the numbers are
but the increasing demand of nuclear energy paves the way forgeneration of even more radioactive waste in future. The conventional methodshave certain limitations. Ion-exchange resins have reported to bedegradedunder high radiation and chemical precipitation has proven to be ineffectivein treating low concentration of radionuclides found in groundwater, posingrisk for centuries (Zhang & Liu, 2020). Remediation methods including soil

Introduction

Since past decades, the application of nanotechnology in the areas of medicine, materials science and electronics have been widely accepted and appreciated due to its potential and benefits.  However, only during the recent years, researchers from other disciplines have started to see the potential applications of nanoscience in the field of agriculture (Robinson and Morrison, 2009). Recently, a generalized and process based framework to enable identification and characterization of the outputs (publications, patents etc.), and map them to the different agricultural research theme areas through the filter of links in the agri-value chain were developed by Kalpana Shastri et al., (2010) from National Academy of Agricultural Research Management (NAARM), Hyderabad, India. The frame work also permits assessing the implications for technology transfer, and impacts on society and environment. This frame work mainly relies on the identification of nano research and relating them agri-food thematic areas (Fig.1).

Introduction

The Indian poultry industry has emerged as one of the fastest growing segments in the Agriculture Sector, contributing significantly to the national GDP. The 20^th Livestock Census in 2019 estimated the total poultry population in the country at 851.81 million with an increment of about 16.8 % over the last one in 2012. The Indian poultry industry is annually growing at around 6.0% for the past four decades and is expected to touch 1066.2 million by 2030. The Per capita availability of meat and eggs is expected to increase accordingly so as to meet requirement projections made by the National Institute of Nutrition (NIN), by the year 2031. 

Introduction

By enhancing microbial populations, enzyme activity, soil respiration, and microbial biomass, nano-black carbon is a novel method for the enhancement of soil biological characteristics. By reducing soil acidity and boosting soil fertility, nano-black carbon is believed to improve plant development. The application of nano-black carbon to agricultural soils can have a significant positive impact on plant productivity. In addition to potentially boosting soil resilience to climate change, nano-black carbon greatly boosted enzyme activity, microbial activity, and soil organic carbon retention.Due to a liming action, nano-black carbon has been proposed as a potential biotechnology to boost agricultural yields in acidic soils.

Introduction

Fertilizers play an important role in improving soil fertility and productivity of crops regardless of nature of cropping sequence or environmental conditions. It has been unequivocally demonstrated that one third of crop productivity is dictated by fertilizers besides influencing use efficiencies of other agri-inputs. In the past four decades, nutrient use efficiency (NUE) of crops had hardly exceeded 35-50%, 18-20% and 30-35 % for N, P and K, respectively, despite our relentless efforts. The remaining nutrients stay in soil or enter into the aquatic environment causing eutrophication. In addition to the low nutrient efficiencies, Indian agriculture facing a problem of low organic matter, imbalanced fertilization and low fertilizer response that eventually caused crop yield stagnation (Biswas and sharma, 2008).

20.1 Introduction
  
Fertilizers play an important role in improving soil fertility and productivity of crops regardless of nature of cropping sequence or environmental conditions. It has been unequivocally demonstrated that one third of crop productivity is dictated by fertilizers besides influencing use efficiencies of other agri-inputs. In the past four decades, nutrient use efficiency (NUE) of crops had hardly exceeded 35-50%, 18-20% and 30-35 % for N, P and K, respectively, despite our relentless efforts. The remaining nutrients stay in soil or enter into the aquatic environment causing eutrophication. In addition to the low nutrient efficiencies, Indian agriculture is facing a problem of low organic matter, imbalanced fertilization and low fertilizer response that eventually caused crop yield stagnation (Biswas and sharma, 2008).The fertilizer response ratio in the irrigated areas of the country  has dcreased from 13.4 kg grain / kg nutrient applied in 1970’s to just 3.7 kg in 2005. In other words, more amounts of fertilizers is required to produce the same quantity of grain output. For instance, 27 kg NPK ha-1 was required to produce one tonne of grain in 1970 while the same level of production can be achieved by 109 kg NPK ha-1 in 2008. 

Nanofibres are an exciting new class of material used for several value added applications in the field of such as medical, filtration as barrier in composites, garments, wipes, insulation, and energy storage. Special properties of nanofibres make them suitable for a wide range of applications from medicine to consumer products and industrial to high-tech applications. The US - National Science Foundation (NSF) defines nanofibres as having at least one dimension of 100 nm or less (Hegde, 2005). Amongst the various nanostructures that have recently been developed for use in practical applications, nanofibres produced from synthetic and natural polymershave received increased attention due to their ease of fabrication and the ability to control their compositional, structural and functional properties (Burgeretal.,2006).

7.1 Introduction

Organic polymers can play important role in ecosystems by accumulating biologically important elements and also by retaining soil moisture after aggregating soil particles. Extracellular polymeric substances (EPS) play an important role in cell aggregation, cell adhesion, and biofilm formation that subsequently protect cells from a hostile environment. Furthermore, certain polysaccharides from microbial sources are surface active, and thus attempts have been made to use them as metal chelaters, emulsifiers and flocculants in industrial and environmental fields/domain. Such use of microbial polysaccharides has infused renewed interest in its production and characteristics.

Nanotechnology offers unique opportunities for the development of novel materials and applications. It is set to offer a platform to revolutionize agriculture sector from production, protection, processing and storage (Kuzma and Verhage, 2006). Bulk of nanomaterials produced is used in other sectors; only ten per cent is of use in food, beverages and packaging sector. Studies have projected the opportunities that nanotechnologies might bring to developing countries in the fields of water, energy, food and agriculture and health (Salamanca- Buentello et al., 2005) The key contributions due to application of nanotechnology in agriculture are delivery systems that aid in slow release and efficient delivery of agroinputs.

Plant disease management technique applicable these days are predominantly relies on hazardous synthetic chemicals that are efficiently deleterious to the human and environment as well. Crop loss every year due to plant pathogen and pest is ranging 30–50%. Nanotechnology, proven advantageous and can offer positive environmental impacts over these harmful chemical pesticides by reducing toxicity, increasing the feasibility of pesticides which are normally baffling in water and improving longevity. Now a days and in coming year, it becoming one of the rapidly growing, advancing and most fascinating technological sciences in many disciplines like medicine, science, technology and more importantly in agriculture, to protect crop from different kinds of pest and pathogen on which most of India’s population is completely dependent. This chapter briefly describe the introduction, role and impact of nanotech in management of plant disease. It is considered as modern technology in facing these widespread destructive challenges and causes in controlling in plant disease. In this chapter we tried to summarize the importance of nanotech in sustainable agriculture and application of various nanoparticles (NPs) in the management of plant disease. NPs can be produced by distinct practices, biological and chemical are one of them important methods. Biological method is widely and commercially used. Also, shortly describe the historical background for some NPs types. This chapter briefly focus on the synthesis of some compounds of NPs, for example silver NPs, silver and silica silver etc and their influence on plant disease also briefly describe the working principle of AgNPs against microorganism. NPs may act in accordance with chemical pesticides. Because of microscopic size, NPs have wide scope in virus control in plant. Now day’s biosensors are used for the quick and precise pathogen detection and diagnosis. Regardless of immense scope of NPs application in managing the diseases of plant, there are precise apprehensions, weakness and risk associated with the utility of NPs in agriculture. It is necessary to be figured out on primacy basis prior to trade of NPs in agriculture.

Introduction

The mankind in the past two centuries has witnessed several technology revolutions: industrial, agricultural, medical, info-tech, biotech etc. Nanotechnology is recent one in the series. Nanotechnology is the manipulation or self-assembly of individual atoms, molecules, or molecular clusters into structures to create materials and devices with new or vastly different properties. It uses techniques, processes and materials at the supramolecular level, approximately in a range between 1-100 nanometres (nm), in order to create new properties and to stimulate particular desired functionalities. For the food industry, nanotechnology applications include: release systems for pesticides or fertilizers in agriculture; antibacterial or easy-to-clean surfaces in food processing machines; food additives such as anti-caking in salt, powders and coffee creamers; anti-foaming agents for beer; natural colour additives for beverages; encapsulated nutraceuticals(antioxidants, vitamins) for dietary supplements; and, micelle systems for low-fat foods. It is predicted that in the foreseeable future nanotechnology will transform the entire food industry, changing the way food is produced, processed, packaged, transported, and consumed. The word “nano,” derived from the Greek “nano” signifying “dwarf,” is becoming increasingly too vast in terms of its huge potential in agriculture, health and the environment. In the agricultural sector, nanotech research and development is likely to facilitate next stage of development of genetically modified crops, farm and animal protection and production inputs, food processing, nutraceuticals etc. India is bestowed with a variety of crops, vegetables, fruits, flowers but food sector is handicapped due to inefficiencies in production, processing, storage and packaging. Most of the processing and packaging in industry and agriculture leaves behind a trail of environmental hazards, which if not tackled at this juncture, will add to long term ecological problems.

Modern agriculture prioritizes intensive crop production in a sustainable manner to meet the growing demand for food. However, the increasing prevalence of biotic stresses such as fungal, bacterial, and viral pathogens, along with insect pests and nematodes, poses a serious threat to agricultural productivity. Conventional pest management practices, rely heavily on synthetic chemicals, that have raised concerns due to environmental toxicity, pest resistance, and health risks. Consequently, scientific community is shifting their focus to ecofriendly alternatives to ensure agricultural sustainability. Plant growth-promoting microorganisms, as bioinoculants have gained widespread recognition for their potential to enhance plant development and reduce the harmful effects of agrochemicals. Despite their promise, the field application of bioinoculants remains limited due to poor shelf-life, rapid degradation and instability under field conditions. The integration of nanotechnology with microbial inoculants, referred to as nanobioinoculants, offers an innovative and sustainable solution to these limitations through the development of smart products, and efficient delivery systems. This chapter highlights the functional synergy between nanomaterials and bioinoculants, integration strategies, mechanisms of action, and their role in managing biotic stresses. Nanobioinoculants, including biogenic nanomaterials, nanobio conjugates, and encapsulated bioinoculants offer enhanced plant protection by producing antimicrobial compounds and

Nanobioinoculants (NBIs) represent a transformative advancement in sustainable agriculture, combining nanotechnology with microbial inoculants to address critical challenges in soil health, nutrient use efficiency, and crop resilience. This chapter explores the mechanisms of action of NBIs, from their precise delivery systems and enhanced microbial-plant interactions to their role in stress mitigation and immune priming. Unlike conventional bioinoculants, NBIs leverage nanoscale carriers to improve microbial survival, enable controlled release, and enhance nutrient uptake, offering superior performance under diverse environmental conditions. We examine their ecological impact, field efficacy, and commercialization challenges while highlighting emerging trends such as AIdriven design and CRISPR-enhanced formulations. By integrating fundamental research with practical applications, this chapter underscores the potential of NBIs to revolutionize precision agrobiology, providing a sustainable alternative to chemical inputs.

Over the years synthetic pesticides like herbicides, insecticides nematicides etc. have been used to improve crop yield. Indiscriminate application of pesticides ultimately leads to their excess discharge into water bodies during rainfall and causes death of fish and other aquatic life. Also consumption of seafood/fishes may cause biomagnification of chemicals in human body and leads to several lethal diseases like cancer, diabetes, neurodegenerative disorders like Parkinson, Alzheimer, and amyotrophic lateral sclerosis (ALS), birth defects and reproductive disorders. Likewise synthetic pesticides also damage soil texture, soil microbes and soil fertility. Their residues enter into plants and animal systems and further move into animals or humans body through food chain.

Abstract

Nanotechnology is an emerging field that offers various potential applications in the areas of agriculture, health and medicine. Recent advances in the design and synthesis of nano-materials aids for developing sophisticated nano-devices for clinical research and practice. Applications of nano materials in medicine includes disease diagnosis, early prognosis, detection of pathogens, tumor detection, tissue engineering, target  specific drug delivery,  development of  biomaterials  for oral health  and understanding of cellular mechanisms in live cells using molecular imaging.

Polymer Clay Nanocomposites

Polymer clay nanocomposites has been used as to affect permeability of the maternal to gases and vapours. Majority of papers on nanocomposites have been focused on the use of smectitic type clays as nanoparticles. They are a group of swelling type of clay minerals including montmorilonite, nontronite, saponite, sauconite and hectorite.

A number of polymer-layered silicates (PLS) preparation methods has been reported in literature. The three most common methods to synthesize PLS nanocomposite are intercalation of suitable monomer and subsequent in situ polymerization, intercalation of polymer from solution and polymer melt intercalation.

In general, layered silicates have layer thickness on the order of 1 nm and a very high aspect ratio (e.g. 10–1000). A few weight percent of layered silicates that are properly dispersed throughout the polymer matrix thus create much higher surface area for polymer/filler interaction as compared to conventional composites. Depending on the strength of interfacial interactions between the polymer matrix and layered silicate (modified or not), three different types of PLS nanocomposites are thermodynamically achievable (Fig. 1 and 2 ) as outlined by Sinha Ray and Okamoto (2003). They are as follows:

Introduction

Nanocomposites are materials that incorporate nanosized particles into a standard material matrix. The addition of nanoparticles can significantly improve properties such as mechanical strength, toughness, electrical or thermal conductivity. Typically, only a small amount of material (0.5-5% by weight) is required due to the effectiveness of the nanoparticles. A nanocomposite is a solid material that consists of multiple phases, where at least one of the phases has one, two, or three dimensions in the nanometer size range. The goal of the nanoscale phase process is to achieve synergy between different constituents. Nanocomposites represent a promising alternative to overcome current limitations of microcomposites and monolithics, and are becoming the materials of the future (Schmidt et al., 2002).

Introduction

Nanofertilizers are products of nanotechnology that are created or modified from conventional fertilizers, bulk fertilizer materials, or plant extracts. They use different methods such as chemical, physical, mechanical, or biological processes to produce nanosized particles that can enhance soil fertility, crop productivity, and quality (KhetiGaadi 2022). Nanofertilizers have a size range of 0.1–100 nm, which is similar to the size of plant cell wall (20 nm), making them easier to be absorbed by plants (Tiwari 2022).

Introduction 
There are terrible consequences of climate change for the planet and all human endeavors. According to statistics provided by the Bureau of Social and Economic Affairs which indicate the rate of increase in global human population is frightening; by 2050 (Rebello et al., 2010), the population is expected to surpass 9.8 billion people. Thus, throughout the course of 30 years from now, the community will encounter several issues that will threaten the survival of the majority of living things on Earth. These issues include the shortage of energy, global warming, greenhouse effects, poisonous gas emissions, and abrupt changes in the environment. The usage of natural fuels as our primary source of energy has raised issues in international scientific organizations, which are working to address one of the key causes of these problems (Zhu et al., 2016). The one method to address the issue is to find an environmentally sustainable fossil fuel replacement that can meet the world’s expanding energy needs (Dincer et al., 2000). Biofuels are one of the viable options and it is an alternative to fossil fuels since they are affordable and harmless to the surroundings (Ma et al., 2014). Various countries around the world are utilizing them to partially substitute the consumption of natural fuels. For example, Brazil uses sugarcane, while parts of Asia and Europe primarily rely on palm oil for production. In the case of biofuels made from microalgae, the United States, Brazil, China, and Japan are the biggest producers. Currently, biofuel generation predominantly relies on three distinct generations of raw organic resources, as outlined by Zhu et al. (2016) and Brennan et al. (2016). These groups represent different sources and technologies utilized to produce biofuels on a global scale. Biofuels classified as first-generation are those derived from feedstocks that are suitable for human intake, such as wheat, sugarcane, maize, palm oil, and sugar beet. Biofuels classified as secondgeneration are derived from lignin and cellulosic feedstock, which consists of non-food crop portions including husks, stems, and leaves that are often thrown away (Kalnes et al., 2007 ; Naik et al., 2010 ; Zhu et al., 2016). However, these biofuel sources can partially fulfill the world’s energy demands. One method to overcome this limitation involves third-generation biofuels, which are generated by cultivating single-celled photosynthetic microorganisms capable of converting carbon dioxide and sunlight into biomass and lipid molecules used in biofuel production (Abomohra et al., 2017).

Nanomaterials are a field which takes a materials science-based approach to nanotechnology. It studies materials with morphological features on the nanoscale, and especially those which have special properties stemming from their nanoscale dimensions. Nanoscale is usually defined as smaller than a one tenth of a micrometer in at least one dimension, though this term is sometimes also used for materials smaller than one micrometer.

An aspect of nanotechnology is the vastly increased ratio of surface area to volume present in many nanoscale materials which makes possible new quantum mechanical effects, for example the “quantum size effect” where the electronic properties of solids are altered with great reductions in particle size. This effect does not come into play by going from macro to micro dimensions. However, it becomes pronounced when the nanometer size range is reached. A certain number of physical properties also alter with the change from macroscopic systems. Novel mechanical properties of nanomaterials are a subject of nanomechanics research. Catalytic activities also reveal new behaviour in the interaction with biomaterials.

Nanomaterials have emerged as promising candidates for revolutionizing sensing and detection technologies, offering unprecedented capabilities in sensitivity, selectivity, and miniaturization. The current chapter provides a concise overview of recent advancements in the utilization of nanomaterials for sensing and detection applications. It encompasses a diverse range of nanomaterials, including nanoparticles, nanotubes, and nanowires, each exhibiting unique properties that enable enhanced performance in sensing applications. The integration of nanomaterials into sensing platforms has demonstrated remarkable progress in various fields, such as environmental monitoring, healthcare diagnostics & security applications.

This section explores how nanoparticles have a significant influence on sensing and detection, explaining how they have revolutionized analytical capabilities. Our trip through many kinds of nanomaterials, such as metal nanoparticles, semiconductor nanomaterials, and carbon-based structures, aims to explore their distinct features and how they might be used for novel sensing methods. The intricacies behind sophisticated sensor technologies are clarified through an examination of plasmonic sensing, quantum dot applications, and conductive nanomaterial responses. In order to improve selectivity, the chapter emphasises surface modifications and biomimetic coatings as key components of strategic functionalization techniques.

Nanomedicine, an interdisciplinary field at the intersection of nanotechnology and medicine, has emerged as a promising avenue for addressing complex health challenges. One area of significant interest is the application of nanotechnology in maintaining and improving gut health. This chapter provides a concise overview of the current state of research in nanomedicine's role in promoting gastrointestinal well-being.

Nanoparticles, with their unique physicochemical properties, offer novel approaches for targeted drug delivery, imaging, and diagnostics in the gastrointestinal tract. Engineered nanoparticles can navigate the harsh conditions of the digestive system, delivering therapeutic agents precisely to the intended sites within the gut.

Nanomedicine is the medical application of nanotechnology. The approaches to nanomedicine range from the medical use of nanomaterials, to nanoelectronic biosensors, and even possible future applications of molecular nanotechnology. Current problems for nanomedicine involve understanding the issues related to toxicity and environmental impact of nanoscale materials.

Nanomedicine research is receiving funding from the US National Institute of Health. Of note is the funding in 2005 of a five-year plan to set up four nanomedicine centers. In April 2006, the journal Nature Materials estimated that130 nanotech-based drugsanddeliverysystems were being developed worldwide.

Nanoparticle characterization is necessary to establish understanding and control of nanoparticle synthesis and applications. Characterization is done by using a variety of different techniques, mainly drawn from materials science. Common techniques are electron microscopy (TEM, SEM), atomic force microscopy (AFM), dynamic light scattering (DLS), x-ray photoelectron spectroscopy (XPS), powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF),ultraviolet-visible spectroscopy and dual polarisation interferometry.

Whilst the theory has been known for over a century (see Robert Brown), the technologyfor Nanoparticle trackinganalysis (NTA) allows direct tracking of the Brownian motion and thismethodtherefore allows the sizing of individual nanoparticles in solution.

Nanomaterials, a cornerstone of nanotechnology, encompass a broad array of particles showcasing remarkable properties and diverse applications. This chapter outlines the classifications and applications of various nanomaterials, including organic, semiconductor, metal, metal oxide, and carbon-based nanoparticles. Each type offers unique characteristics, such as biodegradability, optical and electrical properties, making them valuable in fields like drug delivery, electronics, and catalysis. Furthermore, this study focuses on the interactions of nanoparticles with living organisms, emphasizing their impact on immune systems across plants, invertebrates, and humans. 

14.1 Introduction

The fast growing field of nanotechnology presents great potential to influence various sectors in the areas of energy, environment, agriculture, health care and consumer goods. To overcome the demanding need of nano-based applications, various strategies to synthesize nanoparticles have been attempted. The major focus is to synthesize nanoparticles of desirable properties such as shape, size and compositions. Various strategies have been employed to synthesize nanoparticles. Foremost among these are physical and chemical methods which include sol-gel technique, solvothermal synthesis, chemical reduction, laser ablation and inert gas condensation. But altogether they suffer from process limitations like difficulty in controlling experimental conditions, feeble reproducibility, polydispersity, costly instrumentation and recovery as well as energy intensive and eco-hazardous processes. However, various factors like cost, speed, efficiency, biocompatibility etc. are still not concerned. Thus, finding a balance between scalability, price and applicability is still a true challenge. These limitations can be potentially overcome by using biological methods for nanoparticle synthesis.

Agri-nanotechnology is a new branch of biology that has originated due to the compatibility of nanosized inorganic and organic particles with biological functions. Based on enhanced effectiveness, the new age drugs are nanoparticles of polymers, metals, or ceramics, which can facilitate several biological applications. Plant diseases are caused by pathogens including fungi, bacteria, mycoplasma, viruses and viroids, nematodes, parasitic plants, and protozoa. 

Introduction

Nanotechnology is a scientific and technological field that manipulates matter at the nanoscale to create, design, characterize, and use structures, systems, and devices with novel properties (Sattler 2016). Nanoparticles are one of the most important products of nanotechnology, as they exhibit different features from their bulk counterparts due to their size and shape (Sabir et al., 2014). Nanoparticles have applications in various disciplines, such as materials science, medicine, agriculture, physics, and chemistry.

Synthesis of nanoparticulate metal composites (NMCP) presents a multitude of advantages compared to conventional dosage forms. Acne, a multifactorial skin condition, predominantly involves the pilosebaceous unit and is primarily caused by bacterial species such as Propionibacterium acnes and Acne vulgaris. A staggering 95% of the global population is afflicted by acne. Leveragitng NMCP strategies holds immense promise for innovating novel, efficacious, and low-dose treatments for managing acne. This review delves into various nanotechnological methodologies tailored for effectively addressing acne. Methods: This review encompasses recent advancements pertaining to NMCP carriers aimed at treating acne.

Introduction

Nanopesticides are pesticides that use nanomaterials or nanotechnology to improve their effectiveness and safety in agriculture. Nanomaterials are materials that have at least one dimension between 1 and 200 nm. Nanomaterials have different properties than their bulk counterparts, such as higher surface area, reactivity, and mobility. Nanopesticides can be formulated in various ways, such as nanosized active ingredients, molecular active ingredient nanocarrier complexes, or active ingredients encapsulated or coated by nanomaterials (Deka et al., 2021; Xu et al., 2022).

Introduction

Plastic is the main component of environmental pollution, as it is continuously disposed of into the environment in substantial quantities. Since the creation of the first plastic polymer in the mid-twentieth century, plastic production has increased dramatically, from 1.5 million tons in 1950 to 280 million tons of plastic produced in 2011, with an annual growth rate of 8.7 % (Europe, 2017). By the 2050s, humans are expected to generate 26 billion tons of plastic waste, four times the current levels of plastic waste globally. Due to this serious concern, the United Nations has prioritised plastic waste and its management as one of the fundamental environmental concerns in its 2030 Agenda for Sustainable Development. Plastics appear in the environment in visible and invisible forms. The visible form refers to common scenes, such as bags “flying” on tree branches, PET bottles floating in rivers, lakes, and seas or face masks we use daily. The visible form of plastic in the environment is an aesthetic mockery and a reminder of the socially indiscriminate reception of all offers from the petrochemical industry. The invisible form of plastics is called nanoplastics.

The hazardous toxic metals like cadmium (Cd), arsenic (As), mercury (Hg), lead (Pb), and chromium (Cr), which have detrimental health effects, are naturally discharged into the atmosphere in various ways viz., industrial wastes, anthropogenic sources, etc. Heavy metal pollution of the atmosphere has become a frightening ecological concern that has created serious threats to the ecosystem. These metals have been identified as group I, human carcinogens due to their persistence in the surroundings and bio-magnification in biological systems. Thus, the removal of heavy metals from the atmosphere becomes a serious matter from the biological and environmental point of view. In the past following techniques adoptedfor toxic heavy metals remediation were classified are bioadsorption, ion exchange, membrane filtration, flotation, and solvent extraction techniques.

Nanotechnologies are being spoken of as the driving force behind the new industrial revolution. The mass production of these nano materials and their applications mean a dramatic increase of workers dealing with Engineered Nano Materials (ENM) and becoming possibly exposed to resulting hazardous effects and increased environmental burden of the environment due to the leaks of these materials from industrial processes. It is, hence, essential to consider the importance of ENM safety and their new applications and understand that it is important for these materials and products to be safe to enable successful promotion of nanotechnology for useful applications (Eman M Osman, 2019). 

Characterization Tools: Microscopy

Nanosensors are any biological, chemical, or sugery sensory points used to convey information about nanoparticles to the macroscopic world. Their use mainly include various medicinal purposes and as gateways to building other nanoproducts, such as computer chips that work at the nanoscaleand nanorobots. Presently, there are several ways proposed to make nanosensors, including Top-down and bottom-up design, top-downlithography, bottom-upassembly, and molecular self-assembly.

Predicted applications

Medicinal uses of nanosensors mainly revolve aroundthe potential of nanosensors to accurately identify particular cells or places in the body in need. By measuring changes in volume, concentration, displacement and velocity, gravitational,electrical, and magneticforces, pressure, or temperature of cells in a body, nanosensors may be able to distinguish between and recognize certain cells, most notably those of cancer, at the molecular level in order to deliver medicine or monitor development to specific places in the body. In addition, they may be able to detect macroscopic variations from outside the body and communicate these changes to other nanoproducts working within the body.