eBook Chapters

Introduction

Seed is the key input in agriculture deciding the fate of productivity of any crops. Indeed, seed is referred as a nature’s “nano-gift” for agriculture. Seed is a smart delivery system where we shall make manipulation to achieve the desirable goals. The seed technologists in the country were trying to find a second or third generation technologies to address the issues of seed science. Nanotechnology is one of the cutting edge research areas that has enormous potentials to solve the unresolved field problems. The application of nanotechnology to the agricultural and food industries was first addressed by a United States Department of Agriculture roadmap published in September 2000. Nanotechnology in the agricultural front has been more useful in improving the existing crop management techniques. Most of the applications of nanotechnology spearhead around nano encapsulated agrochemicals, herbicides, nano coated gene delivery and nano fertilizers (Nair et al., 2010).

In recent years application of nanotechnology in various fields including agriculture has received increased attention. Nanotechnology offers an important role in improving the existing crop management techniques especially in plant disease management by controlled and targeted release of agrochemicals to enhance their efficiency. The nano-based delivery systems are beneficial because of improvement in efficacy due to their higher surface area, higher solubility, induction of systemic activity, higher mobility and lower toxicity (Sasson et al., 2007). The pharmacokinetic parameters of nano particles may be altered according to size, shape and surface functionalization. They can also be used to alter the kinetic profiles of drug release leading to more sustained release of drug there by reducing the frequent application (Sharon et al., 2010). The successful use of metal nanoparticles in medical streams as antimicrobial agents has also led to their applications in controlling phyto pathogens (Nair et al., 2010).

Fruits and vegetables are perishables and the post-harvest losses from the point of production at the farm gate till it reaches the consumers is estimated as 30-35 per cent which constitute a major economic drain of more than 33 billion USD equivalent to Rs 2,40,000 crores annually. In order to reduce the losses at various stages, nanotechnologies have come as potential strategies to minimize the losses while improving the marketability and profitability of perishables. One of the plants derived natural compounds “hexanal” which is known to extend the shelf-life of fruits when it is used as a pre-harvest spray or post-harvest dip as a nano-emulsion. Since, it is a highly volatile compound, efforts were undertaken to entrap the compound in the polymer-based nano f ibres (as nano stickers) or as a cyclodextrin inclusion complex (as nano pellets) in order to preserve the perishables. Further, hexanal vapour was also evaluated to preserve fruits. In this book chapter, various nanotechnologies tested, evaluated and technologies developed over the years are elucidated. Overall, the literature review suggests that use of any of the nanotechnologies delivered as pre-harvest spray, post-harvest dip, nano-stickers, nano-pellets and vapour singly or in combination found to reduce the infestation of post harvest diseases, minimize the losses and extended shelf-life by 2-3 weeks that help the farmers to get lucrative prices due to the late arrival in the market besides significantly improve the productivity and profitability of orchards.

Biological diagnostic methods rely on the transmission and amplification of the pathogen in indicator hosts that develop symptoms. These methods test the biological properties of pathogens and early successes in the control of intracellular pathogens were largely due to the diagnosis of the causal agents by biological tests. The sensitivity of biological/serological tests is usually determined by the minimum number of pathogen propagules required to initiate infection. Inoculation with fewer particles could lead to false negative results. Biological techniques for disease diagnosis and pathogen detection are usually highly accurate but too slow and not amenable to large-scale application. 

Summary

Nanotechnology, the science of nanoscale structures has been applied in various fields including in biomedical sciences. Nanotechnology is being increasingly used to create materials and devices so as to interact with the body at sub-cellular scales with a high degree of specificity. Nanotechnology is used in the fight against cancer, cardiac and neurodegenerative disorders, infection and other diseases. This chapter provides an overview of some of the applications of nanotechnology in the field of biomedical sciences particularly with a focus on tissue engineering and medical diagnostics.

Nanotechnology: The Next Big Thing is Really Small

Nanotechnology can be considered as a brainchild of Richard Feynman, as the conceptual underpinings of nanotechnologies were first laid out in 1959 in his historical lecture, ‘ There is plenty of room at the bottom’. The term ‘nanotechnology’ was not used until 1974, when a researcher, Norio Taniguchi, at the University of Tokyo, Japan, used it to refer to the ability to engineer materials precisely at the nanometer level. The prefix ‘nano’ is derived from the Greek word ‘drawf’. One nanometer (nm) is equal to one billionth of a meter or about the width of six carbon atoms or ten water molecules. A human hair is approximately 80,000 nm wide and a red blood cell is approximately 7000 nm wide¹. Nanotechnology can be defined as the science and engineering involved in the design, synthesis, characterization and application of materials and devices whose smallest functional organization in at least one dimension is on the nanometer scale or one billionth of a meter^2-5. It is a multidisciplinary field, which covers a vast, and diverse array of devices derived from Engineering, Physics, Chemistry and Biology and the cumulative efforts of all the disciplines is necessary for any significant outcome.

With the development of improved systems for monitoring environmental conditions and delivering pesticides appropriately, nanotechnology can further improve our understanding of the biology of different crops and thus potentially enhance yields. It can offer routes to plant disease diagnostics, insect-pest management and efficient pesticides utilization. Nanostructure catalysts will help in increasing the efficiency of pesticides allowing lower doses to be used. 

Introduction
Climate change, rapid population growth and limited natural resources such as water and soil pose significant global challenges today. There have never been such drastic repercussions on fruit output worldwide from climate change. In this sense, utilizing methods from the present era of nanotechnology can enhance fruit output and encourage sustainability. Fruit crops have a multitude of roles in world agriculture, making them essential. They supply vital vitamins, minerals and antioxidants that are necessary for maintaining human health and preventing disease. Fruit crops are important players in both local and foreign markets, bringing in large sums of money for farmers and generating a large number of job opportunities, especially in rural areas. Fruit orchards improve soil health and decrease erosion, which promote biodiversity and increase climate resilience. Furthermore, fruits are highly valued on a cultural and social level around the world, enhancing communal customs and culinary traditions. Fruit crops meet nutritional needs by diversifying diets and enhancing food security, which makes them essential
The term ‘nanotechnology’ is derived from the Greek word “nano,” which means dwarf. The development of nanotechnology is closely linked to a historic statement made by Nobel Prize winner Richard Phillips Feynman “There is plenty of room at the bottom”. Designing, characterizing, manufacturing and applying structures, devices and systems by manipulating their size and shape at the nanoscale scale is known as nanotechnology. A nanometer, abbreviated as nm, is one billionth of a meter, or 1×10-9 meters. Nanoparticles are structures with sizes between 1 to 100 nanometers. Nanotechnology may be very helpful in many areas of science and industry by utilizing the special properties of nanoparticles. Understanding and manipulating matter at the nanoscale is of special interest to researchers in the domains of science, medicine, agriculture and industry since materials at this scale frequently exhibit radically different properties from those at larger scales. Nanoparticles find extensive applications in the fields of biotechnology, electronics, textiles, biomedicine, communication technology, biotechnology and renewable energy. In the field of agriculture, innovative nanomaterials have demonstrated great promise for the creation of hybrid crop varieties, new high-efficient agrochemicals for crop protection and nutrition and new crop types through genetic engineering. Additionally, the use of nanomaterials enhances the production of nutraceuticals, the efficiency of pesticides and fertilizers, food processing, packaging, food safety and plant nutrition. It also reduces environmental pollution.

Nanotechnology
Nanotechnology is the study and manipulation of matter at the nanoscale, where unique phenomena allow for novel applications. Nanotechnology is the utilization of components smaller than 100 nanometers for manufacturing and bio-fabrication. This adaptable technique had a wide range of uses, from clothing manufacture to medical advancements such as cancer detection and treatment, among others. The name “nano” comes from Greek, which meaning incredibly small, and a nanometer (nm) is equal to 10 -9 m. Nanotechnology, also called as Molecular Manufacturing based Nanotechnology (MNT) as it involves experimentation and manipulating of nano particles, in order to design and build materials and devices with the basic structure in the nanometer scale. Nano material which can exist in the form of powder or liquid, exhibits significantly different physical and chemical properties at nanoscales compared to their micro size materials with similar chemical composition. Nano food refers to food in nanoparticles, nanotechnology techniques or tools are used during cultivation, production, processing, or packaging of the food.
The development of both organic and inorganic materials achieved through conversion and manipulation of the materials on an atomic and molecular scale [Grumezescu et al., 2018; Dera & Teseme, 2020]. By reducing the particle size below its outset to approximately 1–100 nm, the physical and chemical properties of the materials significantly differed when compared to the original materials despite they contain the same base materials. The unique
and incomparable properties of nanoscale materials are arise due to their high surface area-to-volume ratio compared to the microscale of the same materials [Grumezescu et al., 2018, Berekaa, 2015].

Nanotechnology is a field of study which deals with materials at nano-scale like nanometer which is one billionth of a meter (1 nm = 10-9 meter). At nano-scale, physics, chemistry and biology converge towards the same principles and tools. Nanotechnology is domain in science and technology, which is multidisciplinary in nature and any scientist, engineer, medical doctor can do research on nanotechnology. Presently, it is more of a science and less of a technology because of a lack of proper tools for studying the properties of nano-material. Nano-particles (NPs) are materials that are small enough to fall within the nanometric range, with at least one of their dimensions being less than a hundred nanometres. This reduction in size brings about significant changes in their physical properties with respect to those observed in bulk materials. The presence of naturally occurring NPs is implicated in interplanetary and interstellar space (Hochella Jr, 2002) as well as our earth in which life from the beginning has evolved in their presence (Becker and Reitmeijer, 2006). Currently it is observed that NPs can be synthesized from many biological organisms including plants.

Introduction
Antimicrobial resistance (AMR) is a mounting global crisis that threatens public health, food security, and development. The rapid spread of resistant pathogens has rendered many conventional antibiotics ineffective, leading to longer illnesses, increased mortality, and escalating healthcare costs(1)(2). The World Health Organization characterizes AMR as a top-ten global health threat, driven by overuse, misuse, and inadequate dosing of antibiotics. It already kills 700,000 people a year and is expected to kill 10 million people a year by 2050 if nothing is done about it(3). After penicillin was introduced to the market in 1941, the first signs of resistance appeared in staphylococci, streptococci, and gonococci. By 1942, Staphylococcus aureus, which was penicillin-resistant, emerged(4). Drug resistance must be effectively addressed for a few reasons, including public health, economic considerations, and the long-term viability of medicine. Maintaining the effectiveness of existing treatments for infectious diseases is an important objective(5). NDDS technologies have emerged as powerful tools to enhance the delivery, bioavailability, and targeting of antimicrobial agents, potentially reviving older antibiotics, and maximizing the efficacy of novel compounds(6). Parallelly, AI has found impactful applications in biomedical data analysis, drug discovery, and the rational design of therapies by processing complex datasets, predicting resistance patterns, and guiding the creation of new molecules. The integration of AI with NDDS marks a paradigm shift to optimizes delivery systems, guides individualized treatment plans, and advances the field towards precision medicine for infectious diseases (7). This chapter surveys the convergence of these technologies, exploring how AIenhanced NDDS platforms are reshaping the response to AMR. Additionally, it examines diverse AI models and algorithms employed in the prediction, optimisation, and management of drug resistance, enhanced by real-time case studies for thorough comprehension(fig1.).

History and Recent Developments

Richard P. Feynman in 1959 suggested that it should be possible to build machines small enough to manufacture objects with atomic precision. His talk “There is plenty of Room at the Bottom” is widely considered to be the foreshadowing of Nanotechnology. He delivered this talk at the annual meeting of the American Physical Society at California Institute of Technology (Caltech), In 1990, a team of IBM Physicists revealed that they could write (arrange) the letter IBM using 35 individual atoms of Xenon.

In 1992 a book entitled Nanosystems: Molecular Machinery, Manufacturing and Computation was published by Eric Drexler where he outlined a way to manufacture extremely high performance machines out of molecular carbon lattice (diamondoid).

Around year 2000, federal funding for Nanotechnology in the United States began with National Nanotechnology Initiative (NNI). NNI defined nanotechnology as dealing with materials with sizes between 1 and 100 nanometers exhibiting novel properties.

The Government of India, in May 2007 launched a mission on Nano sceince and nano technology (Nano Mission) with an allocation of `1,000 crores.

Nanotechnology, which deals with understanding and control of matter at dimension of roughly 100 nm and below, has a cross sectoral application and an interdisciplinary orientation. At this scale, the physical, chemical and biological properties of materials differ from the properties of individual atoms and molecules or bulk matter, which enable novel applications. Nanotechnology research and development is directed towards understanding and creating improved materials, devices and systems that exploit these properties as they are discovered and characterized

There are many applications of nanotechnology such as in the area of medicine, chemistry and environment, energy, agriculture, information and communication, heavy industry and consumer goods. The alleged potential of this technology has garnered the attention of both developed and developing countries across the globe. The US National Science Foundation (NSF) has listed it as one of six priority areas; it is one of the themesin the EU Framework Program for Research and Technological Development in Europe; and it has been the focus of research in countries worldwide.

Introduction

Plant diseases are one of the causes of concern in agriculture and horticulture industry. Estimated global crop loss due to plant diseases exceeds hundred billion US$ worldwide (Orke et al. 1994; Narayanasamy, 2010). Plant diseases are caused by microorganism such as fungi, bacteria, viral, viroids and phytoplasma. Most of the plant pathogens are transmitted through seed or planting material, which can spread secondarily through vectors and lead to severe loss to the crop. Among plant diseases, viral diseases cause serious crop losses and affect the quality of products, while the use of virus- free seeds and planting stocks results in a substantial increase in agricultural crop production (Waterworth & Hadidi, 1998; Strange& Scott, 2005).

Indian Agriculture is facing a wide spectrum of constraints such as burgeoning population, shrinking farm land, restricted water availability, imbalanced fertilization, low soil organic carbon, besides experiencing the fatigue of green revolution and vagaries of climate change. To address all the challenges ahead, we should think of an alternate technology such as “nanotechnology” to precisely detect and deliver the correct quantity of nutrients or other inputs required by crops that promote productivity while ensuring environmental safety. The word “nano” refers to the size of one-billionth of a metre or one-millionth of a millimeter in any one of the dimension. We are aware that all materials are made up of atoms which are the smallest units and for example, ten hydrogen atoms put together will measure one nanometer. As the size gets reduced, the surface area gets increased by several folds.

Nanotechnology has the potential to revolutionizethe agricultural andfood industry with newtools forthemoleculartreatmentof diseases, rapid disease detection, enhancing the ability of plants to absorb nutrients etc. Smart sensors and smart delivery systems will help the agricultural industry combat viruses and other crop pathogens. In the near futurenanostructured catalysts will beavailable which will increase the efficiency of pesticides and herbicides, allowing lower doses to be used. Nanotechnology will also protect the environment indirectly through the use of alternative (renewable) energy supplies, and filters or catalysts to reduce pollution and clean-up existing pollutants.

Introduction 
Modern energy policies place a strong emphasis on developing low-carbon energy sources while also ensuring that society has enough energy security. However, because fossil fuels play such a vital part in the global energy system, measures to reduce GHG emissions from fossil fuels must be found. Researchers and scientists are examining the characteristics of materials at the nanoscale level to identify compounds that might boost the efficiency of the energy sources we now use. The physical properties of several materials (such as strength, heat conductivity, and reflectance) alter at the nanoscale in comparison to their bulk properties. When compared to their bulk properties, several materials have distinct physical properties at the nanoscale (such as strength, electrical and thermal conductivity, and reflectance). several materials on a nanoscale. Additionally, materials with nanostructures are more suited for material-to-material interactions due to their higher surface to unit weight volume ratio. This is a useful characteristic as many chemical and electrical reactions take place at surfaces and are greatly impacted by the chemical composition of the reactant and the surface’s structure. The goal of nanotechnology research in the fossil fuel industry is to create novel catalysts that will improve the energy efficiency of the fossil fuel breakdown process. Given that heavy crude oil contains sulphur. The transformation of coal into liquid fuels for use in transportation requires the employment of several kinds of catalysts. Furthermore, because of their structure and the presence of electrons in their outer orbitals, which increases the potential for chemical reactions, the majority of typical catalysts used in cars today rely on valuable metals like platinum. Concerns about the supply of precious metals are raised by the industrialised world’s transport networks’ explosive expansion. Finding more affordable and readily available substitutes is imperative given the scarcity and high cost of these materials, and this will be emphasised in the researchers’ upcoming work. Additionally, because of their unique qualities that relate to the phenomena of corrosion resistance or their exceptionally strong qualities that are advantageous in turbomachinery components, nanomaterials are also helpful in power systems (1) . The application of nanotechnology in the production of resistant ceramics for use as insulators in gearbox lines results in smooth coatings that reduce the likelihood of fouling in cooling water intakes. Additionally, nanotechnology can improve a material’s conductivity and produce a superconductor at ambient temperature rather than a superconducting characteristic at very high temperatures. Renewable energy sources—also known as “green energy”—such as solar, wind, and ocean energy— allow countries to reduce their reliance on petroleum and address environmental problems related to the use of fossil fuels. In these technologies, nanotechnology is also employed. Mao and Chen concentrated their research efforts on developing nanotechnologies for a select group of renewable energy sources, including fuel cells, solar cells, hydrogen storage, and hydrogen generation. This research aims to provide a comprehensive comparative analysis of nanotechnology’s application to the development of sustainable energy systems, including nanomaterials, nanofluids, and nanostructures.

1. Introduction
Aquaculture is rapidly emerging as one of the most important sectors in global food production, supplying an increasing share of the world’s demand for high-quality protein. As global populations rise and overfishing depletes wild fish stocks, aquaculture has become essential in addressing the global food security challenge. The expansion of aquaculture, however, presents a new set of challenges, particularly in the formulation and delivery of efficient and sustainable aquafeeds. Fish and crustaceans, like all living organisms, require precise nutrition for optimal growth, immune function, and reproduction. In commercial aquaculture, ensuring that farmed species receive the right balance of nutrients through formulated feeds is crucial to achieving high productivity and minimizing environmental impact.
`Traditional aquafeeds are designed to meet the nutritional needs of farmed species; however, they face several obstacles when it comes to efficient nutrient delivery. The aquatic environment poses unique challenges for feed stability and nutrient bioavailability, including:
• Water Stability: Aquatic feeds must resist rapid breakdown when exposed to water, ensuring that nutrients remain intact until they are ingested by fish or crustaceans.
• Nutrient Leaching: In water, nutrients can leach out of feeds before they are consumed, reducing their availability and increasing waste.
• Complex Dietary Requirements: Aquatic species have diverse and often complex dietary needs, which must be met through balanced and targeted feed formulations.

Introduction 
The creation of molecular-scale machinery and gadgets at a few nanometers (10^-9 m), known as nanotechnology, has a substantial impact on this topic and dramatically reduces the size of a cell. To assess how nanoparticles affect the synthesis and production of biofuels, particularly biodiesels, a variety of nanomaterials, including nanofibers, nanotubes, and nanometals, are being used (1). Research on biodiesel has shown that utilizing nanotechnology and nanomaterials can be a useful approach to implementing low-cost techniques that improve manufacturing quality. Because of their tiny size and special qualities, such as a high surface area-to-volume ratio, catalytic activity, considerable crystallinity, adsorption capacity, and stability, nanoparticles (NPs) are useful in the generation of biodiesel (2). Because of their high potential recovery properties, metal oxide nanoparticles and carbon nanotubes are frequently used as nanocatalysts in the synthesis of biofuel and biodiesel (3, 4).This chapter critically evaluates the application of nanotechnology in the manufacturing and enhancement of biodiesel, addressing key challenges and potential advancements. Microalgae have been identified as a potentially useful feedstock for biodiesel production. The process of harvesting microalgae can be made much more efficient by employing a range of nanoparticles. Additionally, cost-cutting is facilitated by the recycling of nanomaterials and their incorporation into procedures including cell disruption, extraction, and harvesting. Furthermore, an array of nanocatalysts holds promise for enhancing the efficiency of biodiesel conversion (5). Classification of nanoparticles based on physical attributes as shown in (Fig.1). However, there is a growing emphasis on improving combustion efficiency and reducing harmful emissions in the engine industry and its associated fields. Several studies indicate that the introduction of nanoadditives into blends of diesel and biodiesel fuels has yielded significant results.

Introduction

Off late our understanding of the composition and functions of the human gut microbiota has increased exponentially and there is a great interest in producing healthy foods in particular that which have an impact in improving the gastrointestinal health such as probiotics, prebiotics and synbiotics. Many of these functional foods are dairy based and have been accepted greatly worldwide. Nonertheless, there has been a need for the development of nondairy probiotic, prebiotic and synbiotic products (Salmeron 2017). This has prompted food scientists and food industrialists to study the feasibility of applying other fermenting substrates such as cereals for the development of innovative nondairy fermented functional foods. The importance of including nanoscience and nanotechnology techniques for the creation of fermented cereal beverages that contain specific bioactive nanoparticles and their role in food packaging systems will be discussed.

7.1 Introduction

Food processing has become one of the most competitive sectors in recent years. With increasing consumers’ demands, food industry needs to keep updating technology. These efforts are focussed at improving the organoleptic quality and nutrition of food and improve its keeping quality. This drives the industry to look for newer technologies which include new products as well as new processes. Some of the new processing technologies include non-thermal processes such as high pressure processing, pulsed electric fields and ultrasound processing. Advanced thermal processing techniques such as ohmic heating, inductive heating, extrusion and infrared heating have also been developed. Innovative methods for improving food quality through irradiation, removal of heat and moisture from food are now available. Newer packaging techniques now help to improve shelf-life and attractiveness of the food products and cater to the biodegradability of the packaging material. Hence, there is abundance of new products that have now become part of our daily life.

Food nanotechnology has its long history when the first step of revolution in food processing and improvement in quality of foods was introduced by Pasteur to kill the spoilage bacteria (1000 nm). Later, Watson and Crick’s model of DNA structure, which is about 2.5nm, opened the gateway of applications in biotechnology. Nanotechnology is defined as the design, production, and application of structures, devices, and systems through control of the size and shape of the material at the nanometer (10^-9 of a meter) scale. Nanotechnology is one such technology that has the potential to revolutionize the food industry. Taniguchi first used nanotechnology in 1974 to describe production technology at ultrafine dimensions. The word “nano” means “dwarf” in Greek and one nanometer is equivalent to 10^-9 m. Objects of this scale are not visible through the human eye or the ordinary optical microscope. A nanometer (nm) sized particle measures one billionth of a meter. A human hair measures 80,000 nm while a DNA strand is 2.5 nm wide whereas a protein chain is 5nm in diameter. Nanotechnology can be applied to develop nanoscale materials, controlled delivery systems, contaminant detection and to create nanodevices for molecular and cellular biology. Nanotechnology may be defined as the understanding and management of matter at dimensions of roughly 1-100 nanometers. Unique behavior of matter at the nanoscale enables novel applications. Nanoscale science, engineering, and technology can be used for imaging, measuring, modeling, and manipulating matter at this length scale. Range of sizes of nanomaterials in the food sector and their relative position on nanoscale/microscopic scale are listed below.

Nanotechnology has been successfully applied in various fields such as agriculture, biochemistry and medicine. However, its application in food systems has been new and is yet to be scrutinised. It offers a range of physical and chemical benefits that can be employed in production, development, fabrication, packaging, storage and food distribution systems. These novel nanomaterials such as nanocapsules, nanocomposites, nanoemulsions, nanoencapsulation, nano packaging, nanotubes and nanosensors have been used to improve food quality with better shelf life. However, there is concern about the safety of these nanomaterials, especially because of partial knowledge about the effects of these materials on human health.

Introduction

Manipulating and controlling matter at the nanoscale, usually at dimensions less than 100 nanometers, is the focus of nanotechnology, also abbreviated as “nanotech,” a revolutionary branch of engineering and science. Particular material characteristics and behaviours, drastically different from their macroscopic equivalents, become apparent at this very microscopic size. With nanotechnology, we can design and build buildings, gadgets, and materials with hitherto unseen levels of control and accuracy.

Biocompatible, biodegradable, intelligent and responsive materials are currently an emerging area of interest in the field of efficient, safe, and green pesticide formulation. Using nanotechnology to design and prepare targeted pesticides with environmentally responsive controlled release via compound and chemical modifications has also shown great potential in creating novel formulations. Special attention was paid to intelligent pesticides with precise controlled release modes that can respond to micro-ecological environment changes such as light-sensitivity, thermo-sensitivity, humidity sensitivity, soil pH and enzyme activity (Huang et al., 2018).

Introduction
Eversince green revolution was introduced in 1960’s, the Indian agriculture has taken a series of transformations that resulted in paradigm shift from tradition farming to precision agriculture with an intend to address a wide array of challenges including burgeoning population, shrinking farm land, restricted water availability, imbalanced crop nutrition, multi-nutrient deficiencies, crop yield barriers and decline in soil organic matter. Agricultural scientists were looking for alternative strategy to tideover the bundle constraints with a single stop solution. Nanotechnology deals with atom-by-atom manipulations to evolve processes and products precised to overcome the farming challenges altogether that hardly possible to achieve by the traditional systems. Nanotechnology applications in agriculture include diagnostic devices for early detection of plant diseases (Chen and Hu, 2013), nano-agricultural inputs such as nano-fertilizer (Subramanian et al., 2015; yuvaraj and Subramanian., 2018; Nongbet et al., 2022; Verma et al., 2022; Mohanraj et al., 2024), nano-herbicides (Abigail and Chidhambaram, 2017; Forini et al., 2022) and nano-insecticides (Wang et al., 2022), nano-seed science, plant health management (Subramanian et al., 2016), nano-food systems (Anusuya et al., 2016; Jincy et al., 2017; Jan et al., 2023) besides environmental remediation (Rajkishore et al., 2023).
Even though the nanotechnology is being exploited in wide array of disciplines such as energy, environment, electronics, health sciences and few areas in agricultural sciences, the literature on the application of nanotechnology in plant breeding and genetics is scanty. Nanotechnology is a key component in the creation of genetically enhanced crops (Servin et al., 2015) with an increase crop yield or disease resistance (Kerry et al., 2017). Using genetic engineering or plant breeding, nano-enabled approach aids in the development of resilient and better plant genotypes (Patel et al., 2014). Plant breeding and genetic modification to create better crops frequently utilise nanotech-derived devices (nano sensors and nanoparticles) (Kumar and arora, 2020). Nanobiotechnology is to better understand the biology of various crops and the breeders may be able to increase agricultural yields or nutritional value (Sah et al., 2014). Atomically altered seeds, plant cells with silica beaks, hormone and antibiotic delivery in plants, particle farming, and iron seeding are a few examples. (Prasanna et al., 2018; Jha et al., 2013). Particle farming is the process of cultivating plants in predetermined soils to produce nanoparticles for industrial purposes. Plant production and growth are negatively impacted by abiotic stress. Salinity and drought are the two abiotic stressors that affect plants the most widely (Lavania et al., 2015; Zhao et al., 2017). Nano-enabled crop improvement can be achieved through four important aspects such as (1) genome editing, (2) genetic engineering, (3) abiotic and biotic stress management and (4) plant performance.

The impact of nanotechnology in the food industry has become more apparent over the last few years with the organization of various conferences dedicated to the topic, initiation of consortia for better and safefood, along with increased coveragein themedia. Several companies which were hesitant about revealing their research programmes in nanofood, have now gone public announcing plans to improve existing products and develop new ones to maintain market dominance. The typesof application include:smartpackaging, on demand preservatives, and interactive foods. Building on the concept of “on-demand” food, the idea of interactive food is to allow consumers to modify food depending on their own nutritional needs or tastes. The concept is that thousands of nanocapsules containing flavour or colour enhancers, or added nutritional elements (such as vitamins), would remain dormant in the food and only be released when triggered by the consumer.24 Most of the food giants including Nestle, Kraft, Heinz, and Unilever supportspecific research programmes tocapturea share of the nanofood market in the next decade.

The definition of nanofood is that nanotechnology techniques or tools are used during cultivation, production, processing, or packaging of the food. It does not mean atomically modified foodor foodproduced by nanomachines. Although there are ambitious thoughts of creating molecularfood using nanomachines, this is unrealistic in the foreseeable future. Instead nanotechnologists are moreoptimisticabout thepotential to changethe existing systemof food processingand to ensure the safety of food products, creating a healthy food culture. They are also hopeful of enhancing the nutritional quality of food through selected additives and improvements to the way the body digests and absorbs food. Although some of these goals are further away, the food packaging industry already incorporates nanotechnology in products.

Introduction

Nature has been performing wonderful feats for millions of years. All organisms from microbes to humans are powered by highly evolved molecular and cellular machines that operate at nano scale. The capability to manipulate, control, assemble, functionalise, produce and manufacture things at atomic precision provides an opportunity to understand this new and exciting field of science. Nanotechnology is application of science to control the properties and structure of matter at nanometric scale.

Despite substantial advances in the diagnostics and treatment of diseases, parasitic infections are a major global health issue causing significant mortality and morbidity. Currently, chemotherapeutic antiprotozoal drugs, anthelmintic and anti-ectoparasitic agents are used to treat, but resistance to these drugs has developed due to overuse. In this scenario, nanoparticles are proving a major breakthrough in the treatment and control of parasitic diseases. In the last decade, there has been enormous development in the field of nano medicine for parasitic control. Gold and silver nanoparticles have shown promising results in the treatments of various types ofparasitic infections. These nanoparticles are synthesized through the use of various conventional and molecular technologies and have shown great efficacy. 

Introduction

The act of substituting food or drink with low grade quality ingredients thereby the f inal product will fail to the meet the regulation or standards and in most cases even unfit for human consumption (Augustin et al., 2009). With demand for profit based manufacturing market, there will be increase in points of adulteration with cheaper alternatives or inferior grade ingredients in the production of food or drink (Banti, 2020)]. Adulteration is highly prevails in developing countries such as India, where about 70% of milk supply is adulterated on day to day basis (Bittar et al., 2017). It is every nation and each individual’s responsibility to provide safe and nutritious food to the consumers.

Agriculture serve as a backbone in improving economic growth of a country as well as it provides a base in meeting various needs of people in direct and indirect ways. The recent advancement in nanotechnology has shown a path to develop traditional agriculture into a sustainable practice. Several nanochemicals emerged as promising agents for better plant growth, used as fertilizer, pesticides, control weeds and also used as antimicrobial agents in food packaging. Nanotechnology may act as sensor for checking soil health of agriculture fields.

Nanotechnology is a multidisciplinary domain dedicated to manipulating materials at the nanometre scale (1-100 nanometres), where distinctive physical, chemical, and biological features arise. This transformative technology has applications across various sectors, including medicine, electronics, energy, agriculture, and environmental protection. The concept originated from Richard Feynman’s 1959 lecture, which envisioned the direct manipulation of atoms and molecules. Since then, advancements in microscopy and materials science have propelled nanotechnology into a significant area of applied science. In healthcare, it facilitates targeted drug delivery systems, improving therapeutic efficacy and reducing negative effects. Nanotechnology is also essential in ecological applications, including wastewater treatment and pollution management, where nanomaterials are employed for efficient filtration and remediation. Despite its potential, it raises ethical, safety, and regulatory concerns, including the long-term effects of nanoparticles on health and the environment. The lack of standardized regulations poses challenges for the safe deployment of nanotechnology in various applications. In agriculture, it facilitates the development of nanofertilizers and nano-pesticides, which improve nutrient delivery and reduce environmental impact. Recent research in India highlights the promising use of nano-bionoculants—nano-formulated bio-fertilizers that enhance plant growth and soil health by encapsulating beneficial microbes. Studies have shown that combining nanomaterials with bioinoculants can significantly improve crop yields and resilience to stress, indicating a shift towards sustainable agricultural practices. Nanotechnology represents a paradigm shift in science and technology. It offers innovative solutions to pressing global challenges while necessitating

Nanotechnology epitomizes one of the most dynamic and transformative fields in modern science and technology. This multidisciplinary domain bridges chemistry, physics, biology, along with engineering, enabling the creation of materials and devices with extraordinary properties and applications. The book chapter reflects the research and patenting trends in nanotechnology. USA leading the global share for most patents granted followed by South Korea, Japan, China and Germany for the top five spots contributing more than 76% of all the granted patents by EPO & USPTO related to nanotechnology. Nanotechnological products in relation to sub-industrial areas of medicine, food, agriculture, cosmetics, environment, and renewable energies are also highlighted in chapter. 

Introduction
The aquaculture sector is vital in ensuring global food security by providing a substantial share of the world’s protein supply. With the rising demand for aquaculture products, enhancing fish growth and development has become increasingly important. However, traditional approaches, such as dietary supplements and environmental management, often fall short in terms of efficiency and sustainability. In this regard, nanotechnology presents a groundbreaking solution, with nanomaterials offering innovative methods to overcome these limitations. Nanomaterials possess exceptional
physicochemical properties, including a high surface area, enhanced reactivity, and nanoscale dimensions, making them highly effective in various fields like medicine, agriculture, and environmental management.Their application in aquaculture has demonstrated the potential to enhance nutrient delivery, boost immune system responses, and improve feed utilization, ultimately
promoting better fish growth and health. Additionally, nanotechnology facilitates the targeted delivery of bioactive substances, reducing waste and mitigating environmental impact.This discussion delves into the significant role of nanomaterials in improving fish growth and development, focusing on their mechanisms, advantages, and practical applications. By exploring the interactions between nanomaterials and aquatic biological systems, we can uncover sustainable and innovative solutions to fulfill the increasing requirements of aquaculture sector.
In recent decades, aquaculture is the fastest growing sector and has become a vital food source to meet the global growing demand for protein requirements. This sector provides protein source, healthy fat, and essential micronutrients for the people around the world. It offers local employment, livelihood, high export earnings, supporting national GDP. Improved aquaculture practices
have increased fin and shellfish production, decreasing dependance on wild seed and fish stocks. Global fish consumption increased by 3% between 1961 and 2019 (FAO, 2023). Nanotechnology is a promising solution, offering unique properties at smaller dimensions (1-100 nm) with novel applications (Matteucci et al., 2018; Shaalan et al., 2015). Recent efforts focus on health management, fish and shellfish development, seafood processing, and water treatment.

Nanotechnology is manipulation of matter on an atomic, molecular, and supramolecular scale. The earliest, widespread description of nanotechnology referred to the particular technological goal of precisely manipulating atoms and molecules for fabrication of macroscale products, also now referred to as molecular nanotechnology.
Nanotechnology as the manipulation of matter with at least one dimension sized from 1 to 100 nanometers. This definition reflects the fact that quantum mechanical effects are important at this quantum- realm scale, and so the definition shifted from a particular technological goal to a research category inclusive of all types of research and technologies that deal with the special properties of matter which occur below the given size threshold. It is therefore common to see the plural form "nanotechnologies" as well as "nanoscale technologies" to refer to the broad range of research and applications whose common trait is size.
Introduction
A nanometer (nm) is one thousand millionth of a meter. A single human hair is about 80,000 nm wide; a red blood cell is approximately 7,000 nm wide, a DNA molecule 2 to 2.5 nm, and a water molecule almost 0.3 nm. The term ”nanotechnology” was created by Norio Taniguchi of Tokyo University in 1974 to describe the precision manufacture of materials with nanometer tolerances, but its origins date back to Richard Feynman’s 1959 talk ”There’s Plenty of Room at the Bottom” in which he proposed the direct manipulation of individual atoms as a more powerful form of synthetic chemistry. Eric Drexler of MIT expanded Taniguchi’s definition and popularised nanotechnology in his 1986 book “Engines of Creation: The Coming Era of Nanotechnology”. On a nanoscale, i.e. from around 100 nm down to the size of atoms (approximately 0.2 nm) the properties of materials can be very different from those on a

Nanotoxicity is the study of the toxicity of nanomaterials that focuses on how the size and unique properties of nanoparticles influences their impact on plant helath, human health and the environment. Plants are essential fundamental components of all ecosystems and the interaction between nanoparticles (NPs) and plants is an indispensable aspect of the risk assessment. NP phytotoxicity, an important precondition to promote the application of nanotechnology and to avoid the potential ecological risks was reviewed (Jie Yang et al., 2017). 

Nanotoxicology is the study of the toxicity of nanomaterials. Because of quantum size effects and large surface area, nanomaterials have unique properties compared with their larger counterparts

Nanotoxicology is that branch of bionanoscience, which deals with the study and application of toxicity of nanomaterials. The nanomaterials, even when they are made of inert elements like gold, become very active at a nanometer range. Nanotoxicological studies are intended to determine whether and to what extent these may pose a threat to the environment and to human beings. For instance, Diesel nanoparticles have been found to damage the cardiovascular system in a mouse model.

Human health and safety

Calls for tighter regulation of nanotechnology have occurred alongside a growing debate related to the human health and safety risks associated with nanotechnology. The Royal Society identifies the potential for nanoparticles to penetrate the skin, and recommend that the use of nanoparticles in cosmetics be conditional upon a favorable assessment by the relevant European Commission safety advisory committee. Andrew Maynard also reports that ‘certain nanoparticles may move easily into sensitive lung tissues after inhalation, and cause damage that can lead to chronic breathing problems’.

Cumbu Napier hybrid grass is a cross between Cumbu (Pennisetum glaucum) and Napier grass (P. purpureum Schumach.), widely cultivated across India, Africa, Sri Lanka and South East Asian countries. As the hybrid is a triploid, it displays complete sterility and high vegetative growth. In Tamil Nadu, land area utilized for growing fodder is negligible, accounting only 1.6 per cent of the total cultivated area.

INTRODUCTION

Narcissus is a hardy or tender herbaceous perennial, growing from bulbs and seeds. In India it is commonly called Nargis. The splendid yellow and white flowers of the narcissus alone could make April and May month of delightful sweetness in the garden. Shakespeare mentioned Narcissus again and again as common symbol of the English spring. Narcissus are highly suitable for rowin in beds ots under the deciduous trees, window garden, etc. They are very good cut flower and are sold in the market. The bulbs of the European species are used in folk medicine as these are believed to possess the purgative, dermal and depurative properties. Few species are used for extraction of essential oil in France. There is often confusion as to whether the name narcissus or daffodil should be used. Popular thought attached the former name to the sweet scented tazetta or poet narcissus and the latter to the small wild Lent Lily which is still to be found massed in the wood and open pastures. Today daffodil usually refers to long trumpet and large flowered garden forms while, all the rest are narcissus. In the simplest words, the species of this are called narcissi, although the trumpets, which have trumpet (corona or cup) as long as or longer than the surrounding petals are usually called daffodils.

Introduction

India has one of the best agricultural research systems in the world with better amount man-power amongst the developing countries. The research system includes more than 30,000 scientists along with 1,00,000 subordinate personnel actively engaged in research system in the country involved to agricultural research which considered to be second highest next to China. The achievement and success looks very much impressive and at par with all the developed nations of the world.

National Agricultural System

India has one of the largest agricultural research systems in the world with the largest number of scientific personnel of any developing country except China. The research system includes approximately 30,000 scientists and more than 100,000 supporting staff actively engaged in research related to agriculture. Although the total number of scientists engaged in agricultural research in India looks very impressive, it compares less favorably with many developed countries.

The present agricultural research system comprises essentially two main streams, the ICAR at the national level and the Agricultural Universities at the State level. Besides, several other agencies such as General Universities, Scientific Organizations, and various Ministries/Departments at the Centre, as also Private or Voluntary Organizations participate directly or indirectly in research activities related to agriculture.

National Agriculture Technology Project (NATP)

This project was launched by the ICAR on June 30, 1998, with the support of the World Bank to strengthen and complement the existing resources and to argument the output National Agricultural Research System (NARS).

Objectives

The major objective of this component is:

National Agriculture Development Programme/Rashtriya Krishi Vikas Yojana (RKVY)

Economic reforms initiated since 1991 have put the Indian economy on a higher growth trajectory. Annual growth rate in the total Gross Domestic Product (GDP) has accelerated from below 6 per cent during the initial years of reforms to more than 8 per cent in recent years. The Planning Commission in its approach paper to the Eleventh Five-Year-plan has stated that 9 per cent growth rate in GDP would be feasible during the Eleventh Plan period. However, Agriculture that accounted for more than 30 per cent of total GDP at the beginning of reforms failed to maintain its pre-reform growth. On the contrary, it witnessed a sharp deceleration in growth after the mid-1990s. This happened despite the fact that agricultural productivity in most of the states was quite low as it were, and the potential for the growth of agriculture was high. The GDP of agriculture increased annually at more than 3 per cent during the 1980s.

Since the Ninth Five-Year Plan (1996 to 2001-02), India has been targeting a growth rate of more than 4 per cent in agriculture, but the actual achievement has been much below the target. More than 50 per cent of the workforce of the country depends upon agriculture for it’s livelihood and slow growth in Agriculture and allied sectors can lead to acute stress in the economy because a large proportion of population depend on this sector. A major cause behind the slow growth in agriculture is the consistent decrease in investments in the sector by the state governments. While public and private investments are increasing manifold in sectors such as infrastructure, similar investments are not forthcoming in Agriculture and allied sectors, leading to distress in the community of farmers, especially that of the small and marginal segment. Hence, the need for incentivising states that increase their investments in the Agriculture and allied sectors has been felt.

1. Introduction

The National Agroforestry Policy of India is a comprehensive policy framework designed to improve agricultural livelihoods by maximizing agricultural productivity including animal productivity along with tree cover and mitigating climate change. The Government of India launched the policy in February 2014 during the World Congress on Agroforestry, held in Delhi. The focus of the policy is to address Agroforestry, which is an integrated system of practicing agriculture, livestock farming and forest activities on the same unit of land, storing high carbon, there by mitigating climate change. Agroforestry systems have the potential to be more beneficial than traditional agriculture and forest production practices. They may be able to give enhanced productivity, as well as social, economic, and environmental advantages, as well as a broader variety of ecological products and services. It’s important to remember that these advantages are contingent on appropriate farm management. In 2014 India became the first nation in the world to adopt an inter-sectoral policy on agroforestry, which seeks to identify the bottlenecks in expansion on agroforestry in the country and pathways to remove the constraints in a systematic manner.

India’s growing aquaculture industry requires the implementation of standardized laboratory techniques for detecting fish pathogens to uphold fish health, ensure biosecurity, and meet international trade standards. Both national and international diagnostic guidelines play a vital role in ensuring accurate identification of diseases, surveillance, and certification.
Domestically, the Indian Council of Agricultural Research (ICAR), particularly through institutes like ICAR-Central Institute of Fisheries Education (CIFE) and ICAR-National Bureau of Fish Genetic Resources (NBFGR), has developed diagnostic methods suited for Indian conditions. These include techniques such as histopathology, bacteriology, ELISA, PCR, and qPCR, specifically validated for key cultured species like Labeo rohita, Catla catla, Penaeus vannamei, and Clarias batrachus. The National Surveillance Programme for Aquatic Animal Diseases (NSPAAD) also outlines protocols for disease diagnosis and reporting, aligning with the standards set by the World Organisation for Animal Health (WOAH), previously known as OIE.
At the global level, WOAH offers comprehensive diagnostic manuals for key aquatic animal diseases like white spot disease (WSSV), infectious pancreatic necrosis (IPN), and epizootic ulcerative syndrome (EUS). These manuals detail procedures for collecting samples, using PCR primers, conducting serological tests, and identifying histopathological features. Adhering to these protocols is essential for maintaining the credibility of Indian exports to markets such as the EU, USA, and ASEAN countries. Additionally, laboratories with ISO/IEC 17025 accreditation ensure quality control and global recognition of their test results.

Previous Year Questions

1. Write down the various programmes run by state & central govts for ensuring food security in our country. Briefly discuss the strategies for sustainable agricultural production (20M, CSE 2023)

2. How will national food policy help in sustainable food security? (CSE 2020, 10 marks)

3. Discuss the national initiatives for food and nutrition security. (8M, IFoS-2019)

4. What are the special schemes for providing food grains to underprivileged, destitutes and malnourished populations (CSE 2016, 12.5 marks)

5. National and international food policies. (10M, IFoS-2016)

Historically, Indian scientists have recognized the importance of conserving plant biodiversity with particular reference to indigenous     and naturalized crops and their wild relatives. Indian scientists have recognized the importance of conserving plant biodiversity as early as 1935 by the ‘Crops and Soil Wing’ of the then Board of Agriculture and Animal Husbandry. Late Dr. B. P. Pal, in his classic paper ‘Search for New genes’ published in 1937 emphasized the importance of germplasm augmentation and utilization in crop improvement programmes. The need was reiterated in a meeting of the Indian Society of Genetics and Plant Breeding in 1941, which inter alia discussed the subject of economic crops. Dr. B. P. Pal, working at the then Imperial (now Indian) Agricultural Research Institute approached the then Imperial (now Indian) Council of Agricultural Research (ICAR) to set-up a unit for assembly of global germplasm under phytosanitary conditions in India. The ICAR scheme for plant introduction started functioning in 1946 under the leadership of late Dr. Harbhajan Singh as the first ‘Operational Scientist’. The scheme was further expanded and strengthened as ‘Plant Introduction and Exploration Organization’ in the Botany Division in 1956 and later established as a separate Division of Plant Introduction in IARI in 1961 headed by Dr. Harbhajan Singh.

Introduction
Education and Its Evolution
Education is a process of learning, which involves both formal and informal modes of learning, which transforms the behaviour, skills, knowledge attitudes, and beliefs of the learner. For this reason, it is more deliberate than other education activities since it is a deliberate effort to create learning in a bid to achieve set goals and objectives. Ordinarily, education is characterized by a systematic learning system that is complemented by curricula and instructional materials in different disciplines. This includes talking and explaining, discussing and questioning, performing and observing so as to reach individual students. Communication is also considered as necessary as education is a process of interconnecting people, exchanging information, and learning how to deal with society members. It also develops critical skills such as analysis and developing insights, due to its capacity to offer information to be analyzed, find solutions and then make sound decisions. Education implies character building and it shapes people into responsible members of society. Education also fosters a construct of perpetuity learning which in this case is strengths toward self and career. There is the principle of non-selectiveness as one of the basic principles of education, as education must be provided to all so that all students should be given an equal opportunity. Learners’ understanding and progress can be measured therefore, assessment and evaluation were part of the program to ensure that teaching strategies can be improved. In addition, it has a 96 | Sustainable Development Goals, Health & Education sociocultural function of passing on a culture from one generation to another, and thus preserving social and cultural development and growth. In its strictest sense education is learning as it is getting acquainted with information as well as has the added dimension of moulding positive character in the learners so that they and society can be the better for it. From antiquity to the present, education has undergone tremendous change, having a huge impact on the advancement of society. When education was first developed in ancient communities, it took the form of apprenticeships, oral traditions, and storytelling to impart cultural values, religious convictions, and useful survival skills. For the elite, it was frequently reserved, preventing access for the general public and preserving social hierarchies. A teacher-centered approach limited student interaction and critical thinking, and the curriculum prioritized memorization and rote learning with a focus on mythology and societal responsibilities. In comparison, the goal of contemporary education is to develop students’ ability to think critically, be creative, and solve problems in a formal, structured manner. Regardless of one’s background, access to it is considered a fundamental right that fosters inclusivity and social fairness. The curriculum’s diversity promotes inquiry and discovery by including technology, the arts, and the sciences. A variety of student-centered, modern teaching techniques are used to improve critical thinking and participation. Education is now a key factor in social and economic development as a result of this change, encouraging creativity and producing knowledgeable citizens who take an active role in solving global issues. After all, if education in the past served to uphold hierarchical institutions, education today aims to dismantle obstacles and promote equality in society.

Introduction

The much awaited new educational Policy 2020 approved and released by the Central Government on July, 29, 2020. A document which is set to transform Indian education system in most holistic way and will exert a long lasting influence on framing the Indian education system by addressing the vivid issues across the discipline and streams. The implementation of this policy will be under a single body HECI (Higher Education Council of India) well supported by multiple bodies like National Higher Education Regulatory Council(NHREC), Meta-accrediting body referred as Nation accreditation Council (NAC), Higher Education Grant Council (HEGC), General Education Council(GEC) also called Graduate attributes, it will formulate the National Higher Education Qualification Frame work (NHEQF). This council will take over presently regulating bodies of higher education like University Grant Commission, All India Council of Technical Education and National Council of Teachers Education.

INTRODUCTION

The discipline of Agrometeorology, an applied science is less than fifty years old. Its services are operationally used by individual farmers in developed countries, and by agricultural or development services in more than half of the countries of the world. Its contribution to agricultural production by examining forecast is sought in virtually all countries. In many countries, the respective governments have expressed the desire to use meteorological information to a much larger extent in day-to-day farm planning and operations. The climate database is used for the development of practical criteria in planning and management of irrigated and rainfed crop production system (Martin Smith, 2000).

Though a huge set of Agromet data exists in India, its utility has not been effective due to the scattered location of these data sources and the impediments for free flow of data to the users to analyze and interpret for real-time decision making in agriculture. There is also a considerable time lag between the actual observation and data availability. In view of the importance and the immediate exigency to centralize the database system for helping the researchers and planners, a central agrometeorological databank facility funded by DST from 1998 to 2003 and later by ICAR with the following objectives was established at Central Research Institute for Dryland Agriculture (ICAR), Hyderabad.