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Genetic purity is the vital determinant in continuing the genetic integrity in seed from generation to generation. This is the basis of variety purity maintenance. The genetic purity depends upon the purity of the seeds planted. The genetic purity of the seeds planted must exceed or equal to the final product purity standards required as the purity generally decreases with subsequent generations of propagation. The improved varieties loose their identity and healthiness unless properly maintained. Maintenance selection can prevent the seed quality deterioration.

Clonal crops belong to at least 34 families including herbs, shrubs, trees and vines. Clonal crop improvement dates back to 10,000 years ago. The diversity of clonal crops and their wild relatives are very significant but not yet fully understood and exploited. Many crops represent combination of sexual and clonal reproduction. Different parts of the clonal crops which may or may not be the same as consumed parts have been shaped as clonal propagules such as stem, setts, tuber, rhizome, corms and bulbs among others.

Micrografting is a method to produce virus free, viroid free and disease free grafts that are clonally propagated. This method is an aid to mass multiplication. In vitro grafting is applied to difficult to root clones. Micrografting is performed to understand incompatibility prediction between the two grafting partners, virus indexing, deduction of latent virus, safe germplasm exchange and to do grafting functions throughout the year. It is not season specific. Protocols for micrografting are available for almond, pistachia, olive, prunus, grape, mulberry, cashew, citrus, peach, pear, avocado, hazlenut, walnut, chestnut and grape fruit (Hussein et al., 2014).

In vitro cloning is a vegetative propagation performed in the Plant Tissue Culture system. In vitro micropropagation cloning propagation is mostly applied to the vegetatively propagated plants and in particular in ornamental, fruit and in a few orchard crops and in vegetable crops besides in many forest tree species.

Organically produced seeds are obtained by adapting the organic crop production system. Organic seed is a critical input but specialised efforts to produce organic seeds are not adequately and systematically followed. Accredited organic farming standards recognises the organic seed production as a prerequisite to organic farming. Seed health is important in organic seeds since seeds are not treated with chemical fungicides.

Synthetic seeds are also known as somatic seeds, artificial seeds, clonal seeds and synseeds. They are analogue for botanical seeds. Somatic embryoids which are tissue culture produced embyoids with bipolar structures, apical and basal meristematic regions which are capable of forming shoot and root respectively. Somatic embryoids are encapsulated and are used as artificial seeds possessing the ability to convert into plants in in vitro and ex vitro conditions. Artificial seeds are broadly two types.

Crop improvement is a continuous process. Crop improvement aims to genetically enhance, enrich and empower the crop plants with superior performance, enhanced yield level, higher quality of food, nutritional, fodder, energy produce and adaptability to climate change and crop growing environments. The intrinsic virtues of the improved cultivars could be sustained mainly through the production of the quality assured seeds endowed with genetic capabilities. The quality assured seed delivery system is through the seed chain from the basic seeds through Nucleus-Breeder-Foundation-Certified seed. Quality seed production system need to be based on genetic, breeding and seed technological protocols including field and laboratory testing to ensure very high genetic purity, true to type and superiority.

Deterioration of Crop varieties in their genetic endowments/qualities has been explained by Kadam (1942). Publication on maintenance and management of breeder seed production (Laverack 1991), and maintennce of self pollinated crop varieies (De Hanns 1953; Parlevliet 2007) explain in detail various aspects in maintenance of the seed quality.

Molecular markers may be a short DNA sequence that flanks base pairs like SNP, a Single Nucleotude Polymorphism, small fragment or SSR Single Sequence Repeat or an extended site as in the case of microsatellites. Schematic representation of the SSR assay is shown in Fig. 1. Genetic markers have to be easily identified, linked to a specific locus and polymorphic. Molecular markers are subdivided into codominant monolocus and polyallelic markers and the second group contains dominant, polyallelic and biallelic markers. 

Leaves are alternate in distichous arrangement; lamina long and linear and leaf sheath covering the internodes; ligule membranous and auricle claw shaped. The inflorescence is a spike and florets are borne on rachis node. Each spikelet has three florets. In two row barely, lateral spikelets become sterile.

Agathi is a fast growing tree upto 10m. tall. Branching is drooping. The stem bark is deeply furrowing forming cork like plates. Leaves are compound, pinnae, 20 to 30 cm. long glabresence to pubescent. Leaflet oblong rounded 18 to 24 pairs of leaflets that are green to dark green mostly, bitter free. Inflorescence is a raceme 4 to 7 cm long and bearing 2 to 5 flowers that are white, red, yellowish and pink. Flowers are 5 to 10 cm long, 3cm wide, curved calyx bell shaped 2 cm long, 2 lobed, fine teeth like serration, fleshy. 

Conservation of released varieties requires a sound scientific and technical basis. There are two main strategies for conserving agricultural biodiversity, namely ex-situ and in-situ conservation, both of which are equally important. In-situ conservation refers to the maintenance of a species in its natural habitat. Exsitu conservation is the conservation of components of biodiversity outside their natural habitats. It is conserved in the form of seeds (seed gene bank), live plants (field gene bank), tissues (tissue gene bank), pollens (pollen gene bank) and DNA materials (DNA bank). 

In order to provide for the establishment of an effective system for protection of plant varieties, the rights of farmers and plant breeders and to encourage the development of new varieties of plants it has been considered necessary to recognize and protect the rights of the farmers in respect of their contribution made at any time in conserving, improving and making available plant genetic resources for the development of the new plant varieties. Moreover to accelerate agricultural development, it is necessary to protect plants breeders’ rights to stimulate investment for research and development for the development of new plant varieties.

Food safety and hygiene has been an age-old concern. However, today it has become an important aspect due to the rise in foodborne illnesses for the past few decades globally. It is estimated by WHO that annually 600 million suffer from foodborne diseases as a result of unsafe food consumption while 4,20,000 deaths are recorded globally.

History of food safety starts with knowledge about food hazards. The term “safe food” can be defined from different angles since the end users are highly heterogeneous. However, the accumulating data and day by day emerging information aboutfood borne diseaseslead to a boom in public awareness about food safety. Food safety can be defined as “The strategies and activities aimed to protect foods from biological, chemical, physical, and allergenic hazards that may occur during all stages of production, distribution, and consumption” (Paperella, 2020). According to the World Health Organization (WHO), unsafe food,which contains hazardousmicroorganisms or chemicals, causesmore than 200 ailments, ranging from diarrhoea to malignancies among which diarrhoea is common. 

Food is essential for human survival. It provides the required energy and vital nutrients for sustainable growth and development. With the increase in population and awareness, the demand for nutritious, fresh and healthy food also increases. Although adequate food is produced in order to meet the increased demands, it fails to reach the consumers in need due to food Food Storage and Preservation Practices   35 wastage and spoilage occurring during various stages of harvesting, food production, storage and transportation. Gajanana et al., (2009) stated that there is a mammoth wastage of food post-harvest. It is estimated that 20 to 60 % of produce are wasted during and after post-harvest. 

Safe, wholesome and nutritious food is vital for sustenance, good health, food and nutritional security and augmentation of sustainable development of an individual. Also, safe food helps in the growth of the national economy and enhances the trade and tourism sector. Foods that are unsafe containing various kinds of microorganisms can cause chronic diseases or illnesses varying from diarrhea to cancer.

Growing population has increased the demand on food supply. Providing safe and hygienic food to the population is an essential consideration. Food safety systems and tools need to be in place to assure safe food, until which the food businesses would not flourish or be able to build their reputation. Food safety systems and management tools are important as they assist food business organizations to build confidence in people regarding food safety.

Liberalized economies and evolving businessstrategies push variousindustries to achieve a competitive edge both in the domestic and international markets. Food industry especially, works on the bedrock of quality of products and services in order to attract and retain customers. In this new world of economic order, customers can select from wide range of products and services that are available in the market.

A healthy life is ensured by having access to safe, nutritious and balanced food. World health organisation (WHO) ensures its contribution in developing food safety systems that are sustainable to reduce health risks from farm to consumer. Thus, safe food, its quality and consumer protection are the priorities of this organisation (FAO and WHO, 2021)

Food safety is a discipline ensuring safe consumption of food without any injuries to human health. It is multifaceted and influenced by various factors including, food processing, handling and practices, consumer demand and their knowledge, globalization of world food trade, changes in food consumption pattern (Chen et al., 2020).

Water is an essential component of all living things. Although once believed as a resource with a never-ending supply, it has now been understood that water is becoming increasingly scarce and therefore a proper management is necessary to keep a sustainable food production (Kannan & Anandhi, 2020). A continuous and safe supply of water is indispensable for the food industry where it has numerous applications along the agriculture and food production processes.

All food legislation is predicated on the food law, which protects public health and consumers’ regarding food. The regulation is applied to stages beginning from producing the food, processing and finally distributing the food and feed, apart from domestic use by consumers.

Appealing packaging isthe current trend which influences consumer’s decision to purchase foods. These trends have evolved in the last four decades due to constant and drastic changes in the food packaging processes and mechanisms. These changes have shifted the focus of food packaging from the basic function of containment to extending shelf life.

Nutraceutical is a broad term which represents any substance extracted from food sources with additional health benefits alongside the good nutritional value already present in them. The nutraceutical term was originated by Stephen De Felice in 1989.

At present, food and nutrition security are the prime aspects which need to be addressed in an efficient way. It has been projected that by 2050 the global population will reach 9.2 billion which in turn enhances annual demand for agricultural products by 1.1 per cent from 2005 to 2050 (McKenzie and Williams 2015).

Food provides a sense of wellbeing and comfort and makes people feel good. In this world of a growing population, the need for affordable, safe and good quality food is rising constantly. On the other hand, there is growing awareness of customers who demand that food be produced and supplied hygienically in socially responsible and eco-friendly ways.

Plant diseases are caused by different etiological agents like Fungi, Bacteria, Viruses, Viroids, Phytoplasmas, Rickettsia like bacteria, Nematodes, Algae and Protozoan parasites. To control these specific pathogens and the diseases caused by them, a specific group of medicine/chemical molecule is required.

Fungi are one of the major causes of crop damage which affect crop yield, quality and revenue. Therefore to control these fungal diseases, fungicidal crop protection products are used at different stages of crop growth. Many of our current fungicides produce excellent results with respect to efficacy, crop quality, food safety, and improved cost/profit ratios of agricultural production.

Fungicide which is effective only if applied prior to fungal infection is called a protectant, eg. Zineb, Sulphur. Protectant fungicides are prophylactic in their behaviour.

Actino-IronOG (Streptomyces lydicus WYEC)

Biological soil and seed treatment for Fusarium, Rhizoctonia, Pythium, and Phytophthora with added iron.

There are several fungicidal molecules available in the market, some of which are specific for certain diseases/pathogens while others are broad spectrum and can be used against a range of diseases. However, for proper control measures a knowledge of disease specific fungicide are very important. Ready-Reckoner for disease specific fungicide (Table 4) makes available the option for selection of alternative fungicide in the management of specific disease.

Development of fungicide resistance in a fungal disease pathogen is a major concern for some of the important diseases in their management. This can be overcome by designing of fungicide use strategies to manage the threat of fungicide resistance.

A same active ingredient are available in the market with different trade name and are the product of different companies. These may also differ in their formulation and hazard class (table 7), the knowledge of which is necessary in the plant disease management in view of their effect on the pathogen, ecosystem and environment.

It is used in the control of bacterial leaf spots, blights, and cankers mostly on fruits crops and ornamentals. The Bordeaux mixture, however, can cause burning of leaves or russeting of fruit such as apples when applied in cool, wet weather. The phytotoxicity of Bordeaux is reduced by increasing the ratio of hydrated lime to copper sulfate. Copper is the only ingredient in the Bordeaux mixture that is toxic to pathogens and, sometimes, to plants, whereas the role of lime is primarily that of a “safener.”

Plant viruses cause severe crop losses worldwide. Conventional control strategies, such as cultural methods and biocide applications have limited success at mitigating the impact of plant viruses.

Nematodes are multicellular, unsegmented, bilaterally symmetrical roundworms belonging to pseudocoelomates class of creatures. Many of them are parasites of plants. Different crops species are susceptible to several species of plant parasitic nematodes which limits the yield and quality of the food harvest. Root-knot nematodes (Meloidogyne spp.), foliar nematodes (Aphelenchoides spp.), root-lesion nematodes (Pratylenchus spp.) reniform nematodes (Rotylenchulus spp.) and other ectoparasitic nematodes are the important nematodes species to infect the crop plants.

The algae associated with the plants belongs to the Order Trentepohliales (Ulvophyceae, Chlorophyta). These are filamentous green algae parasitic to higher plants, generally causing minor diseases characterized by negligible leaf spots, but on certain crops, in moist environments, these algal diseases can cause economic injury to plant leaves, fruits, and stems (Nelson, 2008).

Protozoan parasites are Trypanosomatids of the class Kinetoplastida predominately restricted to invertebrate hosts (i.e. possess a monoxenous lifecycle). However, several genera are pathogenic to humans, animals and plants, and have an invertebrate vector that facilitates their transmission (i.e. possess a dixenous life-cycle).

Seed is a basic input to raise the crop plants. Healthy seeds produces the healthy crop. However, the seed carries different pathogens either externally or sometimes internally in the seed to affect the seed emergence and healthy seedling growth. Therefore, the seed treatment with fungicides/bactericides, based on the infection of the pathogen, is highly essential to counter the attack and infection of these pathogens to the germinating seedlings.

Proper selection of plant health medicines like fungicide, bactericide, antibiotics, algaecide, viricide, nematicide, weedicide, plant harmones and auxins and its application at the correct dose and the proper time are highly essential for the management of plant health. The basic requirement of an application method is that it delivers the chemical to the site where the active compound will prevent the pathogen/pest damaging the plant. 

Plant growth regulators or phytohormones are organic substances produced naturally in higher plants, controlling growth or other physiological functions at a site remote from its place of production and active in minute amounts. Thimmann in1948 proposed the term Phytohormone as these hormones are synthesized in plants (Thimmann, 1963).

Anti-transpirants are usually foliar sprays, although they may sometimes be used more conveniently as dips for immersing the above ground plant parts. Foliar sprays may reduce transpiration in three different ways

The use of flower inducers stimulates flower growth during the resting year so the tree produces annually. Flower inducers can also increase the number of viable blooms during the natural fruit producing year, for an increase in production. These can also be used on varieties that provide a crop every year, to boost harvest.

Fruit drop just before harvest is a serious problem for various crop varieties. The severity of fruit drop is influenced by several factors but plant stress and premature ethylene production is at the basis of true physiological drop. Stress factors such as late season heat, primary and secondary pest infestation, and nutrient imbalance or deficiency, and heavy summer pruning, all can contribute to drop.

Sixteen chemical elements are known to be important for a plant growth, their metabolic activities and survival. These can be divided in to two main groups

Medicines used for controlling insect pests are known as Pesicides which have assumed great importance in today’s high yielding and intensive agricultural system as well as public health programme, all over the world. The term pesticide is an all-inclusive word meaning killer of pests and includes substances used for controlling, preventing, destroying, repelling or mitigating any pest.

Pyrethrum is the most widely used botanical insecticide. The active ingredient, pyrethrin, is extracted from a chrysanthemum plant, grown primarily in Kenya, Rwanda, Tanzania and Ecuador. Pyrethrum is the powdered, dried flower head of the pyrethrum daisy, Chrysanthemum cinerariaefolium.

Nereistoxin (NTX) was originated from a marine annelid worm Lumbriconereis heteropoda and its analogue pesticides (table 51) including cartap, bensultap, thiocyclam and thiobensultap have been commonly used in agriculture, because of their low toxicity and high insecticidal activity.