eChapters

There is no terminal of any kind of research, rather they presence a static snap of the gamut of ecological dynamics and functions.

The following scopes for future research

• To construct modelling on resilience, beyond agriculture like livestock f ishery, forestry.

• To create new Algorithm of prediction in ecological behaviour and dynamics

• Gender and economic factors in resilience study and analysis

• Ecological resilience in different agro climatic zones covering coastal ecosystem, Himalayan ecosystem red lateritic ecosystem etc

• Policy research in ensuring and intriguing ecological resilience as well as social resilience on the common platform of production behaviour

• Importance of ecological resilience in the perspective of climate change and global warming

Introduction

My journey into the heart of wildlife conservation began in the unassuming classrooms of KendriyaVidyalaya, where my curiosity for the natural world was nurtured by dedicated teachers and an enriching curriculum. My dreams took f light when I studied stories of Mahadevi Verma in my school days where she was taking care of wild animals in her home. Fuelled by my determination, I pursued my education in Veterinary College, Hebbal, Bangalore from 2006 to 2011. To increase my knowledge and help the wild animals in better way, I did master in Veterinary Microbiology from Veterinary College, Hebbal, Bangalore during 2012-2014, research was conducted in “Salmonellosis in Wild Animals” and PG diploma course in wild animal disease management from TANVAS, Chennai in 2016 research was on “Oral Microflora of Snakes”. Pursing higher studies gave direction to help wild animal, also gave me abilities to write research paper which will help the communities.

Globally the field of veterinary is dominated by women as 55% of veterinarians are women. In India 76% girl graduates were in the year 2021. Lady Veterinarians have been successful at their jobs especially in recent years. This brings out whether there is a psychological relation to this. “Animals are good companions for women.”, said Sreeja Arangottukara to The Hindu.[1] Gender is an identity which develops in a child around the age of 2 or 3 which is influenced by socialisation, culture, and biology.(Penn State College of Liberal Studies)[2] Various psychological researches have shown that women and men differ in intensity of certain attributes. From the ages of 21-64 years assessed with Rytt’s Psychological Well Being Scales and Bem Sex Role Inventory conducted under general Spanish population of 1700 men and 1700 women.(National Library of Medicine) It was found that women were better in positive relations which helps in developing trust between the veterinary team and clients and achieving a positive public perception of the veterinary profession.(today’s veterinary practices)[3] Women are better at understanding facial and body cues which lead them to understand the requirement of the client/patient in medicinal branch of veterinary. Moreover, women are better at verbal skills too their memory and analysing ability makes them capable of research and advancement in the field of animal husbandry. In a profession like veterinary women have better psychological ability and resultingly better at their job.

Introduction

Veterinary Anatomy is regarded as the firm foundation and the knowledge gained in the subject is the basis for reasoning and helping with the diagnosis and treatment of animals. Teaching gross anatomy is highly regarded as being clinically relevant. The research on a wide range of species in Veterinary anatomy will be helping to be the understanding of their biology. The research conducted helps to understand the anatomy of domestic and wild animals and how this can be applied to diagnostic imaging and other disciplines of Veterinary Science. Hence teaching and research in anatomy provides foundation on which the knowledge of clinical practice and medicine is built. The research in Anatomy pave way for advanced research in other Veterinary Discipline.

TEACHING PERSPECTIVES

Anatomy education has entered newer and more innovative methods of teaching. “Vesalius” referred to as a founder of modern Anatomy struggled to make dissection of cadavers mandatory in 16th century and its significance is again being questioned today in search of substitutes. Hence use of alternatives to dissected specimens and multimedia teaching methodologies to teach anatomy and basic principles of Veterinary Medicine and Veterinary Surgery have gradually become more popular.

The Indian subcontinent has a vast costal belt along with the wide range of forest environment, which host large number of unexplored plant/marine species. This diversity has been the source of unique chemical compounds with the potential for industrial development as pharmaceuticals, cosmetics, nutritional supplements, molecular probes, fine chemicals and agrochemicals. In recent years, a significant number of novel metabolites with potent pharmacological properties have been discovered from the natural sources including marine and terrestrial plants. Recent move of society towards nature for the treatment of various diseases where there is no satisfactory cure in modern medicine has diverted the attention of natural/medicinal chemists and biologists to unravel their chemical characteristics and biological activities together in order to define their therapeutic potential in the light of modern pathobiological understandings. This move has led collectively to rediscover, design and refine the therapeutic application of medicinal plants/marine sources.

Vultures are obligate and highly efficient scavengers. They are keystone species providing important ecosystem services, but their adaptations to save energy, gorge themselves up to one third their body weight at a time, survive easily without food for up to a fortnight, conserve energy by using soaring and gliding flight, are just some of the unique and important characteristics of these magnificent birds. A highly efficient digestive system helps in extracting nutrients from otherwise non-nutrient sources like hide, tendons, and even bones. Vultures have been instrumental in keeping India clean and safe from disease outbreaks. A bird which is unaffected by multiple disease-causing micro-organisms helps to rid the environment from disease pandemics and epidemics. However, since the mid 1990ss these robust birds with a life span of 40-60 years, have declined drastically, from tens of millions to a few thousand currently, a decline of around 99%. For the formerly most abundant of these vultures, the white-rumped vulture, declines already happened by 99.9% in just 15 years! These declines led to multiple problems including increases in feral dog populations, economic and social losses and most importantly, 5,00,000 human deathsin five years,as a direct cause of the decline of vultures. The main cause of the vulture declines has been scientifically proven to be due to the use of veterinary diclofenac in cattle, their major source offood. Although veterinary diclofenac was banned since 2006, the drug has remained in the cattle carcasses at lower levels and new vulture toxic drugs have been discovered.

The global herbal industry faces significant challenges due to varying regulatory frameworks, quality control standards, and traditional practices. This talk aims to bridge the gap by promoting “Herbal Harmony”- a unified approach to standardizing herbal products worldwide. Experts from diverse f ields will converge to discuss: Harmonization of regulatory frameworks, standardization of quality control and assurance, validation of traditional knowledge, innovative technologies for herbal analysis and global market trends and opportunities by fostering collaboration and knowledge sharing, “Herbal Harmony” seeks to: enhance consumer safety and confidence, promote sustainable herbal trade practices, support evidence-based herbal medicine and to facilitate international collaboration and trade.

Present work was undertaken to study the effect of doxycycline and doramectin inmale rats. Following exposure to doxycycline hyclate orally for 28 days at dose rate of 10,16.50 and 33 mg/kg, rats of any doxycycline treated groups did not show any clinicalsigns, mortality or abnormal behaviour. Percent body weight gain was significantlyreduced in rats of group treated with doxycycline (16.50 and 33 mg/kg). A significantincrease in relative weight of left and right testes was found in rats exposed to doxycycline(33 mg/kg). Doxycycline did not produce any change in haematological parameters in ratsof doxycycline treated groups. Blood glucose level, total cholesterol, LDL cholesterol andBUN levels was significantly increased in rats of group exposed to doxycycline (33mg/kg). Rats of group treated with doxycycline (16.50 mg/ kg) exhibited a significantdecrease in blood HDL cholesterol level. Hepatic SOD level was significantly increased inrats of group treated with doxycycline (33 mg/kg).

Endometritis is one of the leading causes of reproductive inefficiency, infertility and decreased milk yield in high yielding dairy cows and buffaloes as well as in other species of animals and are associate with severe economic loss. Indiscriminate use of antimicrobial agents has resulted in development of resistance to the synthetic antimicrobials. Plant-derived active principles have promising potential to act as an alternative to conventional antimicrobial agents. In the present study, an attempt was made to investigate the mechanism of action of Prosopis juliflora leaves (PJ) extract against clinical isolates of E. coli.. Out of 42 clinical samples of uterine discharges and 23 isolates from cattle and buffaloes having history of uterine infection, we identified two isolates as virulent E. coli based on the cultural, biochemical and genotypic characterization. Ethanolic PJ extract showed presence of large quantity of total phenolic acid and flavonoid contents as well as promising in vitro antioxidant and radical scavenging activity. PJ extract exhibited a marked in vitro antibacterial effect against S4 isolate and reference strain (ATCC 25922) as evidenced by agar well diffusion test with MIC value of 0.39 mg/ml. This antibacterial action of PJ extract was shown to initiate at 6 h post-exposure while the complete bactericidal action was achieved at 12 h post-exposure.

Endometritis is one of the leading causes of reproductive inefficiency, infertility and decreased milk yield in high yielding dairy cows and buffaloes as well as in other species of animals and are associate with severe economic loss. Indiscriminate use of antimicrobial agents has resulted in development of resistance to the available antimicrobials. Plant-derived active principles have promising potential to act as an alternative to conventional antimicrobial agents. In the present study, an attempt was made to investigate the mechanism of action of Eucalyptus robusta leaves (ER) extract against resistant bacterial isolates. Out of 70 clinical samples of uterine discharges from cattle and buffaloes having history of uterine infection, we have identified one isolate as methicillin-resistant Staphylococcus. As per the CLSI guideline, this isolate (S4) was found to be catalase +ve, oxidase –ve and possess mecA and coaA genes along with shown resistance to cefoxitin and MIC breakpoint for oxacillin was >4 µg/ml. Methanolic extract of ER reconstituted in water showed presence of eucalyptol (marker compound) along with other major bioactive phytoconstituents following GC-MS analysis. ER extract exhibited marked in vitro antibacterial effect against S4 isolate and MRSA (ATCC 33591) as evidenced by agar well diffusion test with MIC value of 0.3125 mg/ml.

The objective of the present study was to explore the therapeutic potential of hot alcoholic (100%), hydroalcoholic (70%) and cold aqueous extracts of Pterocarpus marsupium and hot aqueous extract of ITK formulation (mixture of gum acacia, black cumin, wheat and barley) against obese streptozotocin induced type 2 diabetes in male Wistar rats. In Phase I study, tested extracts were observed to possess carbohydrates, alkaloids, proteins and amino acids, tannins, flavonoids, saponins, fixed oils and resins with additional glycosides in ITK formulation after screening by qualitative tests and further confirmed by semiquantitative analysis (by Gas Chromatography-Mass Spectrometry) to possess active principles having antidiabetic, antioxidant, nephroprotective, cardioprotective and anti-inflammatory properties. The extracts were evaluated for in vitro antioxidant (2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and 2, 2’-Azino bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assay) and antidiabetic (α-amylase and α-glucosidase inhibitory activity) was determined. Aqueous and hydroalcoholic extracts have potent antioxidant and alcoholic extract of P. Marsupium showed superior antidiabetic activity over ITK formulation. Further in Phase II study, 48 obese male Wistar rats were divided into eight groups viz. group I (Healthy Control), group II (Obese), group III (Obese Diabetic), group IV (Obese Diabetic + Metformin @ 50 mg/ kg b.wt.), group V (Obese Diabetic + ITK @ 435 mg/kg b.wt), group VI (Obese Diabetic + Aqueous P.marsupium @ 1 g/kg b.wt) and group VII and VIII were treated with hydroalcoholic and alcoholic @ 300 mg/kg b.wt.

Present study was undertaken to unravel the influence of experimentally induced pre- existing diabetes on survival time and/or mortality pattern in septic animals and how diabetes and sepsis, when occur concurrently, alter vascular reactivity of aorta. Type-1 diabetes was induced following intraperitoneal injection of streptozotocin (STZ: @ 65 mg/kg) for 5 consecutive days while sepsis was induced by caecal ligation and puncture (CLP). Streptozotocin produced a sustained hyperglycaemia in mice and there was decrease in body weight with progression of hyperglycaemic state. CLP caused hyperglycaemia followed by euglycaemia and hypoglycaemia, while in diabeto-sepsis hypoglycaemia was set earlier, leading to early mortality. Alteration in vascular reactivity and endothelial dysfunction was observed.

The present study was designed to assess the cardio-protective role of oleic acid in myocardial injury. Myocardial injury was induced in rats by intra-peritoneal injection of isoprenaline (ISO; @ 110 mg/kg b.wt) for two consecutive days at 24 h interval. Oleic acid was administered orally (@ 5 mg/kg b.wt or 10 mg/kg b.wt) for 21 days before inducing myocardial injury to evaluate its ameliorative potential. Samples (blood, heart) were collected from different groups of experimental animals 24h after last injection of isoprenaline. Besides evaluation of heart weight to body weight (HW/BW) ratio, myocardial infarct size, oxidative stress parameters and haemato-biochemical parameters, cardio specific biomarkers of injury, ECG, isolated right atrial response and mRNA expression of gene coding for cardiac uncoupling protein-2 (UCP-2) were also quantified. Isoprenaline administration significantly increased the HW/ BW ratio, myocardial infarct size, lipid profiles (total cholesterol, HDL-C, triglyceride) in ISO-induced myocardial injured rats.

The objective of the present study was to explore the therapeutic potential of Gymnema sylvestre and ITK formulation against obese streptozotocin-induced type II diabetes and diabetic cardiomyopathy in male Wistar rats. In Phase I study, 80% hot methanolic extract of Gymnema sylvestre leaves and hot aqueous extract of ITK formulation (ingredients gum acacia, black cumin, wheat and barley) was prepared and evaluated for in vitro antioxidant and antidiabetic activity of extracts. Extracts have potent antioxidant (studied by 2, 2-Diphenyl 1-picrylhydrazyl (DPPH) and 2, 2’-Azino-bis (3-ethylbenzothiazoline-6 sulphonic acid) (ABTS) radical scavenging assay), antidiabetic (determined by in vitro α-amylase and α-glucosidase inhibitory activity) and glucose uptake potential in cultured lymphocytes and were observed to possess alkaloids, f lavonoids, saponins, anthraquinones and phenols in Gymnema sylvestre leaves with additional resins, tanins, proteins, glycosides and fixed oils and fat in ITK formulation after screening by qualitative tests and further confirmed to possess active principles having antidiabetic, antioxidant, cardioprotective, nephroprotective, and anti-inflammatory properties.

The present study was carried out to assess the role of endogenous cannabinoids in regulating aortic vascular response during early and late phases of sepsis. Polymicrobial sepsis was induced by caecal ligation and puncture (CLP) in mice. Besides, recording the isometric tension in arterial rings, estimation of haemato-biochemical parameters, histopathological examinations of vital organs, mRNA expression of CB1 receptor and MAGL enzyme and their role in the vasoconstrictor response to noradrenaline in the aorta of septic mouse were studied. Sepsis significantly reduced RBC, Hb and WBC counts during both early (CLP-6h) and late (CLP-20h) phases of sepsis whereas neutrophil count was increased during early phase. There was also a marked fall in lymphocyte count during late phase of sepsis indicative of immunosuppressive state. Significant rise in the plasma ALT, AST, BUN and creatinine levels during early and late phases of sepsis were suggestive of liver and kidney dysfunctions which were further substantiated byhistopathological examinations of these vital organs. Sepsis produced a state of hypoproteinaemia with significant reduction in plasma albumin level.

Present study was embarked upon to extricate the underlying calcium signaling mechanisms responsible for spasmogens (PGF2α , and oxytocin) induced muscular contractions in oviducts of non-pregnant buffalo in oestrous stage. Isometric tension in longitudinal oviductal in both ampulla and isthmus strips was recorded under the resting tension of 1 ± 0.2 g following mounting the tissue in Ringer–locke solution. Following an equilibration period of about 120 to 150 min, oviductal strips both ampulla and isthmus presented a consistent and rhythmic pattern of spontaneity. Mean Integral Tension (MIT) and amplitude (g) of ampulla and isthmus in normal spontaneous tissue did not differ significantly. The frequency (BPM) of ampulla and isthmus in normal spontaneous tissue differed significantly (P<0.05). PG2α produced significantly (P<0.05) greater contraction than oxytocin in both ampulla and isthmus of buffalo oviduct in concentration-dependent manner. In isthmus the contraction produced by oxytocin was negligible. PGF2α produced a significant change in tonic contraction between ampulla and isthmus but the change in phasic contraction between ampulla and isthmus was not significant. PGF2α produced significantly (P<0.05) greater contraction in ampulla than in the isthmus of buffalo oviduct.

Ornithosis

Ornithosis is caused by Chlamydia and characterized by enlargement of liver and spleen, serofibrinous pericarditis, air sacculitis and enteritis. This is also known as chlamydiosis, psittacosis and is more common in parrots, pigeons and other psittacine birds.

Etiology

• Chlamydia psittaci group B

• Size 0.3 to 1.5 m

• Disease spreads from wild birds

Spirochetosis

Spirochetosis is caused by a spiral shaped organism Borrelia anserina and characterized by greenish diarrhoea, enlarged and mottled spleen, small necrotic patches on liver and linear haemorrhage in proventriculus. This disease is transmitted by a tick Argas persicus and thus also known as “tick fever” or “tick paralysis”.

Etiology

• Spirochete Borrelia anserina

• Spiral shape 8-24m long and 0.2 to 0.3 m wide microorganism having 8-11 spirals.

• Stained with aniline dyes

As the chill of winter fades away, and nature begins to bloom, it’s time to embrace the joys of spring with your furry friend by your side. While spring brings about a sense of renewal and excitement, it also brings some unique challenges for pet owners. Here are some essential tips to ensure your pet dog stays happy, healthy, and safe during the spring season:

1. Regular Grooming Sessions: Springtime means shedding season for many dogs, as they lose their winter coats to make way for lighter ones. Regular grooming sessions, including brushing and bathing, can help reduce shedding and prevent matting. Additionally, grooming helps in checking for any ticks, fleas, or skin irritations that may arise as the weather warms up.

2. Watch Out for Allergies: Just like humans, dogs can suffer from seasonal allergies too. Keep an eye out for signs such as excessive scratching, licking, or sneezing. Consult your veterinarian if you suspect your dog is experiencing allergies, as they can recommend appropriate treatment options or medication.

3. Protect Against Fleas and Ticks: Springtime brings a surge in flea and tick activity. Ensure your dog is up to date on flea and tick prevention medications recommended by your veterinarian. Regularly inspect your pet for any signs of these pests, especially after outdoor activities in grassy or wooded areas.

4. Stay Hydrated: Warmer weather means increased activity levels for both you and your dog. Make sure your pet has access to clean and fresh water at all times, especially during outdoor excursions. Dehydration can be a serious concern, so carry water and a portable bowl when going for walks or hikes.

As the temperature drops and snowflakes begin to fall, it’s essential to ensure that your beloved canine companion stays warm, healthy, and happy during the winter months. While winter brings its own set of challenges for pet owners, with some extra care and attention, you can make sure your pet thrives even in the coldest of seasons. Here are some essential tips for caring for and managing your pet dog during winter:

1. Provide Adequate Shelter: If your dog spends time outdoors, make sure they have a warm and dry shelter to retreat to when the weather gets harsh. Whether it’s a sturdy doghouse or a cozy insulated shelter, ensure it provides protection from wind, rain, snow, and low temperatures. Consider adding blankets or straw bedding for extra warmth.

2. Limit Outdoor Exposure: While dogs may enjoy playing in the snow, it’s crucial to limit their time outdoors during extreme cold weather. Frostbite and hypothermia are real dangers for dogs, especially those with short fur or small breeds. Keep outdoor activities short and supervise your dog closely to prevent any weather-related emergencies.

3. Protect Their Paws: Snow, ice, and road salt can be harsh on your dog’s paws, causing irritation, dryness, and even chemical burns. Invest in a pair of dog booties to protect their paws during walks or consider using paw balms to moisturize and create a protective barrier. After walks, wipe your dog’s paws with a warm, damp cloth to remove any salt or ice-melting chemicals.

The eyes and ears are not only vital sensory organs for your pet dog but also key indicators of their overall health and well-being. Proper care and management of these delicate areas are essential for preventing infections, maintaining comfort, and preserving your furry friend’s quality of life. Here’s a comprehensive guide to eye and ear care for pet dogs:

Eye Care

1. Regular Inspection

• Start by regularly inspecting your dog’s eyes for any signs of redness, swelling, discharge, or cloudiness. Clear, bright eyes indicate good health, while any changes may signal an underlying issue.

• Look out for excessive tearing, squinting, or rubbing of the eyes, as these may indicate irritation or injury.

1. The word physiology was first used by Greeks around 600BC to describe a philosophical enquiry into natural things.

2. The word "physis" meaning nature.

3. The discovery of blood circulation by William Harvey in 1628.

4. C.Bernad (181378) who is considered as one of the founder of physiology.

5. The process of transport of an oxygen from outside air into the cells within tissues are called as Respiration.

6. STPD is abbreviated as standard pressure dry.

7. The gill arch along with the filaments is called as gill.

8. Haemoglobin is abbreviated as Hb or Hgb.

9. Cephalopods use haemocyanin- a copper containing protein for oxygen transportation.

10. Protobranch - the gill structure tends to occur in primitive groups and appears as a small leaf like structure.

1. The removal of moister from the timber is known as seasoning.

2. For boat building purpose timber should have moisture about 18-20%.

3. Mild steel contain 0.15-0.30% of carbon.

4. Low carbon steel contain less than 0.15% of carbon.

5. High carbon steel contain 0.8-1.5% of carbon.

6. The specific gravity of steel 7.84.

7. The aluminum boat was firstly built in France 1892.

8. Ferro cement boat was introduced in France 1847.

9. Specific gravity of aluminum-2.7.

10. Specific gravity of Ferro cement 2.4-2.6.

1. The most disaster affected populations in the world - China,

2. Heavy droughts usually occur in Afghanistan.

3. Heavy human causalities due to flood occur in Cambodia.

4. Ring of fire an arc of volcanoes and fault lines encircling the Pacific basin.

5. Occurrence of unusual cold wave Russia.

6. 22 Indian states are prone to disasters.

7. Pre-Disaster mitigation can help in ensuring faster recovery from impacts of disaster.

8. IOC-International Oceanographic Commission.

9. USGS-United States Geological Survey.

10. NOAA-National Oceanic and Atmospheric Administrations.

1. The word Extension was first used in U.S.A.

2. The word Extension is derived from Latin meaning out stretching.

3. Extension education = non formal education.

4. Extension work out of school system of education.

5. Learning active process on the part of the learner.

6. Motivation need satisfy goal-seeking behaviour.

7. Steps in extension teaching methods = AIDCAS.

8. Teaching is the process of providing situations in which learning takes place.

9. Learning is the process by which a person becomes changes in his behaviour through self-activity.

10. Lecture is the best method for presenting information to large number of persons in a short period of time.

1. Project monitoring is an integral part of project management.

2. Concurrent evaluation is carried out during implementation of the project.

3. Terminal evaluation is undertaken at the time of completion of the project.

4. Ex-post evaluation is also called as impact evaluation.

5. Network techniques are used for minimising cost and time of the project.

6. PERT-Program Evaluation and Review Technique.

7. LOT-Latest Occurrence Time.

8. CPM-Critical Path Method.

9. Project presentation - 30 pages.

10. National level project goal of economic growth.

1. The word "Statistics" derived from the Latin word 'Status' meaning POLITICAL STATE.

2. Statistics are lifeblood of successful commerce.

3. Statistics are not suitable to study the qualitative phenomenon.

4. Statistics laws are not exact.

5. A.L.Bowley "Statistics are numeral statement of facts in any department of enquiry placed in relation to each other".

6. Croxton and Cowden "Statistics may be defined as the science of collection, presentation analysis and interpretation of numerical data from the logical analysis".

7. Alfred Marshall - "Statistics are the straw only which I like every other economist has to make the bricks".

8. Function of statistics

a. Condensation

b. Comparison

c. Forecasting

d. Estimation

e. Tests of hypothesis

9. ANOVA-Analysis of Variance.

10. ANOVA-developed by Prof. R.A. Fisher.

The seed yield, along with the rate of application and uptake of nutrients, is used to determine the following parameters for nitrogen or other nutrients:

Partial Factor Productivity (PFP) (kg seed/kg N applied): Partial Factor Productivity (PFP) is a measure used in agriculture to assess the efficiency of nutrient use, particularly nitrogen or other essential nutrients, in crop production. It is calculated as the ratio of the seed yield to the rate of nutrient application. The formula for PFP is:

*A higher PFP value indicates greater efficiency, meaning that more crop yield is produced per unit of nutrient applied. This is desirable as it implies that the nutrient is being used more effectively by the crop.

Agronomic Efficiency (AE) (kg seed/kg N applied): Agronomic Efficiency is a measure used to evaluate the effectiveness of nutrient application in increasing crop yield. It specifically assesses the yield gain per unit of nutrient applied. The formula for AE is:

The Dumas combustion method, named after the renowned French chemist Jean-Baptiste Dumas, is a widely recognized analytical technique employed in the determination of the elemental composition of organic compounds. This method involves the complete combustion of a sample in an oxygen rich environment, followed by the separation and subsequent analysis of the resulting combustion products.

Principle

The Dumas combustion method involves the complete combustion of a sample in the presence of excess oxygen. The nitrogen present in the organic compound is converted into nitrogen gas (N2 ), which is then measured quantitatively. The process can be summarized as follows:

i. Combustion: The sample is combusted at high temperatures (typically around 900-1000°C) in the presence of oxygen. This results in the conversion of all organic material into carbon dioxide (CO2 ), water (H2 O), and nitrogen gas (N2 ).

ii. Removal of Interfering Gases: The combustion products are passed through various reagents to remove interfering gases such as CO2 and H2 O.

iii. Detection of Nitrogen: The nitrogen gas is then measured, often using a thermal conductivity detector (TCD) or another suitable detection method.

Black Pepper and Pearl Millet Flour for Disease Control in Chickens: To treat a disease in chickens characterized by frothing at the mouth, frequent cackling, restlessness, and reduced food intake, 10-15 black pepper corns are ground and mixed with pearl millet flour and water to form small pellets. These pellets are fed to the chickens to increase body heat and help eliminate the disease (Sabarkantha, Gujarat) (Parmar, 1992a).

Use of Garlic Against Pulorum in Poultry Birds: To cure chicks affected by Salmonella pulorum, one or two peeled cloves of garlic (Allium sativa) are soaked in a cup of water for 12 hours. The water is then given to the chicks to drink, curing them in two days (Dahod, Gujarat) (Khant, 1999).

Poultry Disease Management: For treating an unidentified disease in poultry birds that kills them within a few days, tribals use various plant-based remedies:

1. Bark of ‘beda’

2. Small pieces of onion

3. Grains of paddy smeared with kerosene

4. Tuber of ‘dev-alad’ (Curcuma spp)

a) A Key GDP Contributor: Dairy and Livestock sector form the backbone of agriculture with 28 per cent contribution in GDP and the progress in this sector is dependent on innovative measures to increase the value for farmers as well as customers.

b) Doubling Farmer’s Income : Doubling Farmer’s Income by 2022 can be achieved by improving crop and livestock productivity, efficiency to utilize resources, increased intensity of cultivation and diversification in high value cash crops, processing and value addition of food and animal products. This price realization can be assured through market reforms and efficient market linkages.

c) Unorganised sector – a challenge : The Indian dairy sector is still characterized by strong presence of unorganized sector. Apart from the Cooperative dairy sector, in the last few years there has been a considerable growth in Private Dairy sector and Milk Producers Organization like Maahi in Gujarat. If small dairy farmers are provided with sufficient ‘value addition’ training and support, they can increase their income considerably.

d) Value addition : Further, the milk processing plants can also go for value addition and innovation and in turn pay remunerative prices to milk producers.

The publication of “Flora of British India” by Hooker (1872-1897) was followed by a series of regional floras by Cooke (1901-08) for Bombay presidency; Prawn (1903) for Bengal: Duthie (completed by Parker & Turrill, 1905-29) for upper gangetic plains and adjacent Siwalik and of sub Himalayan tracts; Kanjilal (1966) for the forest flora of Uttar Pradesh; Gamble (completed by Fisher, 1915-1936) for Presidency of Madras; Haines (1921-1925) for Bihar and Orissa: and Kanji et al (1934-1940) for Assam. Duthie’s work was completed by Raizada and associates (Raizada, 1954, Raizada et al. 1961; 1983; Raizada & Jain, 1964, 1966). Later, Raizada (1976) brought together all additions made to Duthie Flora. However, the trends of additions has persisted afterwards and is hoped to continue onwards as well. Revision of old floras has become all the more important because the nomenclature of a large number of species has changed in accordance with International Code of Botanical Nomenclature (1956, 1961, 1966, 1972, 1978 & 1983).

Jaggery or Gur: Unrefined brown coloured sugar produced mainly in India by evaporation of sugar cane juices. Or a crude brown sugar made from the sap of coconut or palmyra palm trees, sold in round cakes or lumps.
Jam: A fruit preserve made by mixing fruit or boiling it with sugar and water and adding extra gelling agent if required, usually pectin. The high sugar concentration inhibits but does not totally prevent proliferation of microorganisms, so jam is usually bottled hot in sterile jars with a seal, or sterilized after bottling.
Jasmine: Natural flavourings with warm, spicy characteristics derived from flowers and leaves of jasmine (Jasminus spp.). Predominant flavour compounds and aroma compounds include jasmonates, jasmones, benzyl acetate, indol and eugenol.
Jellied milk: Milk to which is added sugar, flavourings, thickening agents and gelling agents. It is also known as jellified milk.
Jellies: Small, soft sweets, usually fruit flavoured, of gelatinous texture, made in various shapes and often coated with sugar. The singular term, jelly, is used to refer to jam-like products, usually clear, that are made from strained fruits containing pectins which are boiled with sugar. Also refers to soft, semi- transparent foods prepared from gelatin which are sweetened, flavoured, cooled in a mould and eaten as desserts.

Quality assurance: Planned and systematic actions necessary to provide adequate confidence that goods or services will satisfy given requirements. For the food industry, this is a customer-focused management system, whose aim is to guarantee food safety and con- sistent product quality by application of production, processing and handling standards. Proactive food safety programmes, in particular those based
on Hazard Analysis Critical Control Point (HACCP) principles, are the foundation of many food quality as- surance systems.
Quality control: A system of maintaining standards in manufactured products by testing a sample against the specification.
Quantitative descriptive analysis: Comprehensive system used in sensory analysis that covers sample collection, assessor screening, vocabulary development, testing and data analysis. Quantitative descriptive analysis (commonly abbreviated to QDA) uses small numbers of highly trained assessors. Once the training sessions have established satisfactory panel performance, and removal of ambiguities and misunderstandings, the test samples can be evaluated. This is carried out in replicated sessions using experimental designs that minimize biases. Three major steps are required: development of standardized vocabulary; quantification of selected sensory
characteristics; and analysis of results by parametric statistics.

UHT milk: Milk heated by UHT treatment to prolong shelf life.it is also known as long life milk.
Ultra High Temperature (UHT) treatment: A heat treatment (direct or indirect) used to sterilize foods prior to packaging in which to kill all microorganisms that would otherwise spoil the product. Following UHT treatment, foods are filled into pre-sterilized containers in a sterile atmosphere. Food products processed by UHT treatment include liquid products (e.g. milk, some fruit juices, cream, yoghurt, wines, salad dressings), foods with discrete particles (e.g. infant foods, tomato products, some fruit juices and vegetable juices, soups), and foods containing larger particles (e.g. stews).
Ultracentrifugation: Centrifugation in centrifuges which have the ability to develop centrifugal fields of up to 100,000 times that of the gravitational field. Ultracentrifugation is generally used for analytical purposes, such as the determination of physico-chemical properties of food polysaccharides using sedimentation analysis.
Ultrafiltration: Selective membrane separation process, driven by a pressure gradient, in which suspended solids, colloids, emulsified solids such as fat-protein complexes, and dissolved macromolecules with molecular weight in the range 10,000-100,000 Da are retained by the membranes. Molecules that do not pass through the membranes constitute the retentate. Lower molecular weight dissolved materials that pass through the membrane under a driving force of relatively low hydrostatic pressure (1-10 bar) are the permeate. Ultrafiltration is generally used in the concentration and fractionation of large molecules from materials such as cheese whey and milk.

Xanthan gums: Gums produced by the bacterium Xanthomonas campestris. These gums are exo-polysaccharides composed of repeating pentasaccharide units comprising a cellulose backbone and trisaccharide side chains of D-mannose and D-glucuronic acid residues. The gums also contain variable quantities of pyruvic acid. It used widely in the food industry as thickeners due to their ability to produce highly viscous, highly stable aqueous solutions. Other uses include as emulsifiers, stabilizers and binding agents, and to provide body, e.g. in low fat foods.
Xanthophylls: Group of neutral yellow or brown carotenoid pigments that are oxygenated derivatives of carotenes and distributed widely in plants. It is useful as food colorants.
X-ray fluorescence spectroscopy: Spectroscopy technique in which the sample is irradiated with X-rays, causing emission of a characteristic X-ray photon and fluorescence, which is measured using a spectrophotometer.
X-rays: Penetrating electromagnetic radiation of very short wavelength, able to pass through many materials. X-rays are produced by bombarding a target, usually made of tungsten, with high-speed electrons. The shorter the wavelength of the X-ray, the greater is its energy and its penetrating power. Longer wavelengths, near the UVray band of the electromagnetic spectrum, are known as soft X-rays. The shorter wavelengths, closer to and overlapping the gamma-ray range, are called hard X-rays. A mixture of many different wavelengths is known as white X-rays, as opposed to monochromatic X-rays, which represent only a single wavelength. X-rays are used in the food industry for a wide range of analytical purposes, including detection of foreign.

Yakult: Yakult Original is a fermented milk drink that contains over 650 crores of beneficial bacteria called Lactobacillus casei Shirota (SHIROTA strain). It has a unique sweet and tangy taste and is enjoyed by people of all ages. It is one of the probiotic foods that is drunk to help maintain the health of the gastrointestinal tract. It has benefits like (i) Improves Digestion: The Unique bacteria in Yakult Original helps to balance the gut microbiota, aiding digestion and promoting regular bowel movements. (ii) Boosts Immunity: Regular consumption can enhance the body’s natural defences by stimulating the immune system. (iii) Supports Gut Health: By increasing the number of good bacteria in the gut, Yakult Original helps maintain a healthy digestive system.
Yeast biomass: Quantitative estimate of the total population of yeasts present in a given habitat, in terms of mass, volume or energy.
Yeast extracts: Water soluble fraction of autolysed yeasts. During autolysis, yeast enzymes hydrolyse cytoplasmic proteins and carbohydrates. Insoluble cell wall material (cellulose) is removed, e.g. by centrifugation, to leave a clear extract of water soluble cellular material that is rich in amino acids and other nutrients. Yeast extracts are used as flavourings, as a source of nutrients for microbial fermentation, and as a source of B group vitamins for fortification of foods.

Zein: Prolamin which accounts for approximately half of the total storage proteins in corn. Contains minimal concentrations of lysine and tryptophan, but is rich in leucine.
Zeolites: Crystalline, hydrated alkali-aluminium silicates. Useful as catalysts for production of invert sugar from sucrose, downstream processing of flavour compounds, detoxification of contaminated foods and feeds, and as molecular sieves.
Zero energy cool chamber (ZECC): Zero energy cool chambers is an on-farm rural oriented storage structure which operates on the principle of evaporative cooling and has been constructed using locally available raw materials such as bricks, sand, bamboo, rice straw, vetiver grass, jute cloth etc. The chamber has been constructed above the ground and comprises of a double-walled structure made up of bricks. The cavity of the double wall is filled with riverbed sand. The upper part of the chamber was covered with vetiver grass mat on a bamboo frame. Zero energy cool chamber (ZECC) is a double wall structure having space between the walls which is filled with porous water absorbing materials. These pads are kept constantly wet by applying water. When unsaturated air passes through wet pad, transfer of mass and heat takes place and the energy for the evaporation process comes from the air stream. Losses can be minimized by using best post-harvest handling techniques during storage, transportation and distribution to market. There are various technologies available to create and maintain optimal temperature, relative humidity and atmospheric composition for harvested fruits and vegetables during storage.

The separation of essential oils responsible for odour/fine smell from different plantparts by gentle heating is known by centuries ago. A large number of such components being volatile are called as ‘Essential oils’. Presently, it also covers many of the less volatile constituents. The essential oils (extracts of the plant) with different types of flavours/odours are aromatic components, which can be separated by steam/hydro-distillation or extraction by solvents.
Essential oils largely contain terpenic compounds, made up of two or more isoprenyl units (C-5). Besides, a large number of other compounds which have nothing to do with isoprene units, have also been reported by various workers. Essential oils are of quite use in perfumes, flavour and aroma-therapy industries and their economic production is very much essential from farmers’ point of view. The optimum stage of harvest is very crucial for high quality and quantity of the essential oil from different parts of the plants (leaves, inflorescence, entire herb, roots, seeds, rhizomes etc.).
The quality of the essential oils largely depends on the kinds of the metals (copper, aluminium, mild steel, stainless steel) used in distillation unit, as some of the essential oils react with free acids present in the oil and thus imparting off-smell of aroma and colouration. Presently, distillation units made up of stainless steel are preferred which has no reaction to phenolic, acids, etc., and oils produced are very clean, less coloured and of high quality preferred by the cosmetic and perfumery industries engaged in formulation of various types of essence and scents.

Aromatic plants are one of the major natural resources of our country occurring in diverse eco-system. Lately, many people due to rare chances of side effects prefer herbal medicines/remedies and their safer products. Aromatic plants sector of our country is not well organized which needs to be given special attention in States as well as National level. At present in India, total trade of Ayurveda and other herbal
products is estimated to be around 5000 crores, which indicates that medicinal and aromatic plants have good potential. Presently, 90 percent of traded medicinal plants are generally collected from wild and rest from cultivation. These are either used fresh or in the form of crude drug, either dried whole plant or their part/parts such as root, stem, wood, bark, leaves, flowers, fruits, seeds and their products. The major producers of medicinal products in the world are China, Brazil, Bulgaria, Poland and India.
Though India has rich medicinal and aromatic plant diversity, yet China is the largest exporter of medicinal plants earning huge amount (US $ 5 million) from herbal trade due to large scale cultivation, standardization of quality control, safe and regular suppliers to the international market. Despite being home for several Traditional System of Medicines and aroma industries, the export of herbal based products from India is below US $ 100 million. According to WHO estimates, the herbal and aroma market is growing at the rate of 7 percent annually. The major export market for India is USA, Japan, Germany, France, Australia etc.

1. Introduction
All glassware used for bacteriological work must be meticulously cleaned and sterilized. Used and soiled glassware should be thoroughly washed to remove dirt and solid residues before drying and undergoing sterilization.
2. Cleaning of New Glassware
Borosilicate glass (e.g., Pyrex) and factory-washed soda glass require only standard washing before use. However, new, unwashed soda glass needs additional treatment to neutralize alkali content.
• Place the items (e.g., test tubes, Petri dishes, and flasks) in a suitable container, cover with a 1% tri-sodium phosphate solution, and bring to a boil.
• Remove the items, rinse with tap water, and immerse them in a 1% hydrochloric acid solution to neutralize alkalinity.
• Rinse thoroughly with tap water, followed by distilled water. Allow the glassware to dry before sterilization.
3. Cleaning of Used Glassware and Other Apparatus
• Autoclave all glassware contaminated by microorganisms (121°C for 15–20 minutes). This liquefies solid media, facilitating removal.
• Rinse the empty glassware with tap water, then warm water (50–55°C).
• Soak in a detergent solution (e.g., 1% washing soda or tri-sodium phosphate solution) and scrub with a brush.
• Rinse thoroughly with tap water to remove detergent traces, followed by distilled water.

Introduction
Ocular micrometer and stage micrometer are used to measure the size of microorganisms. The Ocular micrometer consists of a circular disk of glass which has graduations engraved on surface. A stage micrometer or objective micrometer has scribed lines on it that are exactly 0.01mm (10 micrometer) apart. Ocular micrometer is calibrated for the objective first. To calibrate the ocular micrometer for a given objective, the two scales are superimposed and determined how many of the ocular gradation coincide with one graduation on the stage micrometer scales. Thus, the distance between the graduations on the ocular micrometer can be determined and so the size of the organism can be determined.
Materials
1. Ocular micrometer
2. Stage micrometer
3. Microscope
4. Stained slides of yeast or bacteria

Introduction
This method consists of mixing the microorganisms in a small amount of India ink or nigrosine and spreading the mixture over a clean slide. These two pigments are not really bacterial stain and do not penetrate the microorganisms. These pigments make the background dark thus making organisms transparent against dark background. This method is useful for determining cell morphology and accurate size as no shrinkage of cells due to heat.
Materials
1. Bacterial culture (B. subtilis)
2. Microscopic slides (Clean, Grease Free)
3. Inoculating needle
4. Cedar wood oil
5. Microscope
6. Xylene

Fruit firmness is an important indicator of maturity. During ripening, fruits may soften and become too soft at the overripe stage. The changes are detected by pressing the fruit in between hands or by using devices like a pressure tester or texture analyzer to measure the texture of fruits and vegetables. Commonly used pressure testers to measure the firmness of fruits and vegetables are the Magness–Taylor Pressure Tester and Penetrometer. Principle: For the measurement of pressure, the maximum force required to rupture the sample is measured by pushing a cylindrical metal probe into the sample to a given depth. The Magness-Taylor pressure tester can be used to determine the softening of fruits during maturity. In this instrument 10 lb or 30 lb force springs are available. Each of them is provided with one or two punches of 5/16 or 7/16 inch in diameter. The plunger of larger diameter is used on softer material, while; the smaller one is used for firmer material.
Procedure
1. Select the pressure tester according to type of fruit
2. Remove a thin portion of the skin in case of waxy fruits

Measurements of sugars in the fruit can indicate the stage of maturity. Total soluble solid (TSS) contents can give a reliable measure of the sugar and other water-soluble components in fruits, vegetables, and their products such as juice, squash, jam, preserve, candy, sauce, ketchup, etc., In climacteric fruits, carbohydrates accumulate during maturation in the form of starch, and as the fruit ripens the starch is broken down into sugar. In non-climacteric fruits, sugar tends to accumulate during maturation. The presence of sugars, acid, ascorbic acid, and other water-soluble components in the fruit, vegetable, and their products are measured as total soluble solids.
Principle
Soluble solids can be determined using a refractometer which measures the refractive index or a brix hydrometer which measures specific gravity. A Brix hydrometer is used to determine the percentage of brix in syrup while an Abbe refractometer gives both refractive index as well as degree brix. The Refractive index of the test solution is measured at 20°C using a refractometer and the soluble solid content is calculated by the use of tables correlating the refractive index with soluble solids.

The inorganic residue remaining after the destruction/burning of organic matter in foodstuffs is called ash. Ash contents in food commodities roughly represent the mineral contents, however, some minerals are likely to be lost due to volatilization or some interaction between constituents during ashing. Estimation of ash content is also used as a measure to judge the quality of fruit products. The presence of high ash content or low alkalinity of the suggests the presence of adulterants in the sample. Similarly, the presence of a high amount of acid-insoluble ash indicates the presence of sand and other silicious matter in the food products.
Principle
Organic matter is burnt off at as low a temperature as possible. Heating is done in stages, first to char the product thoroughly and finally to ash it at 550ºC in a muffle furnace. The inorganic material left after burning organic matter is cooled and weighed.
Apparatus
* Silica Crucibles
* Muffle furnace
* Desiccator
* Balance

The use of salt (Sodium Chloride) in food products is an important method of preservation. It not only imparts taste to the products but also helps in checking spoilage of fruit products. Salt is added to pickles, sauces, chutneys, ketchup, and many other products. Food Safety & Standards Regulation has specified the maximum limit of salt in different products. Therefore, the estimation of salt content in food products is considered as an important attribute in food quality control.
Direct Titration Method
Direct titration (Mohr’s) method is used for the determination of salt content in food products as a routine quality control. In this method, the sample is titrated against a standard solution of silver nitrate for the estimation of salt.
Reagents
0.1 N NaOH, Phenolphthalein 1% indicator solution
0.1N Silver nitrate (AgNO3)
5% Potassium Chromate

Starch is the storage form of carbohydrates in plants which is abundantly found in roots, tubers, stems, fruits, and cereals. It is a polysaccharide made up of glucose monomers with a chemical formula of (C6H10O5)n. Starch is composed ofamylose and amylopectin. The linear polymer amylose is the most basic form of starch while amylopectin is the branched form. Foods that are high in starch content are a good source of nutrition. Food starches are used in the food industry as thickeners and stabilizers. During the ripening of fruits, the starch is converted into sugars. Therefore, the presence of starch in fruits is used as an indication of maturity and ripening.
Principle: For estimation of starch contents, the sugars present in the sample are leached out and starch is hydrolyzed using either acid or enzymatic hydrolysis. Stach is then calculated as invert sugars.
Starch content by Acid Hydrolysis method
Reagents: Ethanol 95%, /50%,Concentrated HCl, Concentrated H2SO4, 1N NaOH solution, Phenolphthalein indicator, 10% alcoholic solution of Alphanaphthol.

Fruit pulps are semi-finished products that are used for manufacturing finished value-added products like jam, ready-to-serve beverages, squash, appetizers etc. According to Food Laws, pulp refers to an unfermented but fermentable product, pulpy, turbid or clear, obtained by a mechanical process from sound ripe fruits/vegetables. These are preserved either by heat processing or by using chemical preservatives. The maximum preservative permitted under Indian Food Laws is 1000 ppm sulphur dioxide added in the form of potassium metabisulphite.
Mango, guava, apple, pineapple, papaya, bael, guava, peach, plum, apricot etc., are the most suitable fruits for making pulp for use in finished product preparation.
Procedure
i. Select fully mature and ripe fruit like mango, guava, apple, peach, apricot, kiwi, etc., and wash thoroughly.