eChapters

Introduction

Ever since the civilization of mankind, efforts have been directed towards accumulating and storing foods when they are in plenty in order to meet needs during the days of scarcity. In case of food grains not much problem was faced due to nature’s noble way of reducing the moisture level as the grains mature. However, in case of fruits and vegetables, long-term storage in their fresh form was not possible (until development of modern methods) primarily due to their high degree of perishability owing to high moisture content of these commodities at the time of harvest.

Appropriate Post Harvest Management (PHM) of horticultural crops fruits and vegetables in their fresh form prolongs their usefulness and in some cases improves their quality. It also checks market glut, helps in orderly marketing, increases financial gain to the producers and preserves quality of produce for much longer time. Horticultural produce like fruits, vegetables and cut flowers are living, respiring tissues separated from the parent plant. Therefore, the aim of PHM is to control various physiological processes viz. respiration, transpiration and other metabolic activities to keep produce in maximum usable form.

Introduction

In India Agriculture was practiced formerly on a subsistence basis; the villages were self sufficient, people exchanged their goods, and services within the village on a barter basis. With the improvement in rural infrastructure all these goods and services has become commercial in character, resulting in development of Micro-enterprises and requirement of their business promotion.
Businesses with five or fewer employees, called micro-enterprises, support many rural and urban households in developing nations. The term marketing connotes a series of activities involved in moving the goods from the point of production to the point of consumption. 
Marketing is an integral part of micro-enterprise products to encourage them to make more investment and to increase production. Thus there is an increasing awareness that it is not enough just to produce however it must be marketed as well.

Introduction

With the major thrust of extension agencies now focused on production techniques, the market-led extension holds the key to the future. This assumes greater significance in the light of the new international trading regime under WTO as the export opportunities are now being opened-up to India, which may have direct implications on agro-enterprise development.

In the light of the above, the extension functionaries in India are presently ill-equipped to deal with such challenges. It appears now that the production of the agri-produce is going to be dictated by market requirements. In this age of WTO, the extension methodology also needs to work out for dissemination of good agricultural practices to the farmers based on internationally accepted standards. This recommendation has now been made in the 05th National Extension Education Congress held in 2009 jointly conducted by Indian Council of Agricultural Research (ICAR) and CS Azad University of Agriculture and Technology, Kanpur (Proceedings of the 05th National Extension Education Congress, March 05-09, 2009, ICAR and CSAU, Kanpur). 

A. Answer the following questions:

1.    What is proximate analysis?

Ans: Proximate analysisis a systemforapproximatingthenutritive valueof a feedstuff without actually usingit in a feedingtrial.

2.    Why proximate analysis is called Weende analysis?

Ans: In 1865, two scientists namely Wilhelm Henneberg and FriedrichStohmanndeviseda methodcalledWeende analysis/ proximate analysis at Weende Experiment Station, near the University of Goettingen, Germany. Hence, it is called ‘Weende Analysis’.

3.    Write the six proximate principles?

Ans: Water (moisture), ether extract (crude fat), crude fibre, total ash, crude protein and nitrogen free extract (NFE).

4.    Why ether extract is also called as crude fat?

Ans: As it is composed of true fats (glycerides of fattyacids) along with other substances like hydrocarbons, free fatty acids, fat solublevitamins,pigmentslike carotene & chlorophyll,waxes, sterols,lecithin(phospholipids), alkali substances,volatile oils and resins.

5.    What is NFE?

A. Answer the following question

1.    Writethe two assumptions whicharetaken into consideration during CP estimation of any feedstuff?

Ans: In the feed, a) all N is present as protein and b) All food protein contains 16%nitrogen.

2.    What is protein equivalent?

Ans: It is the arithmetic mean of the percentage of DCP and DTP (digestible true protein).

A. Answer the following questions

1.    What is adulteration?

Ans: It is an intentional admixture of a pure substance with some cheaper and low quality ingredients in order to make more profit.

2.    What is sampler?

Ans: The appliance used for the collection of samples is knownas sampler.

3.    What is dry rolling?

Ans: The process of disintegration of kernels into particles with the application of pressure by moving rollers is known as dry rolling or cracking.

4.    What is crimping?

Ans: The process of rolling of feed ingredients with the use of corrugated rollers is called crimping

5.    What is crumbles?

A. Answer the following question

1.    What is ANF?

Ans: The substances that are naturally present in the feed which by themselves or by their metabolic products interfere with the feed utilization (FCR), reduce production and affect the health of the animal are called anti-nutritional factors.

2.    How does lectin cause agglutination?

Ans: Lectins have the affinityfor certain sugar molecules. Hence, it combines with the glycoprotein components of RBC and there bycausing agglutination of the RBC.

3.    Why ruminants are more resistant to gossypol toxicity?

Ans: It is due to formationof stablecomplexeswith soluble protein in rumen which are resistant to enzymatic breakdown.

4.    Why ruminants of Hawaii and Indonesia are not susceptible to mimosine toxicity?

Ans: Ruminants of Hawaii and Indonesia possess ruminal bacteria which can rapidly degrade 3-hydroxy-4(1H)-pyridone (3,4 DHP) and thus are not susceptible to mimosine toxicity.

5.    How saponin form stable foam?

A. Answer the following?

1.    How antibiotic increases nutrient absorption from the gut?

Ans: In antibiotic fed animals, the intestine becomes thinner and healthier, thus they helps in more absorption of nutrient.

2.    What is probiotic?

Ans: Probioticcan be defined asthe live microbial food supplement that beneficially affects the host animal by improving the intestinalmicrobial balance.

3.    What is prebiotic?

Ans: These are the compound other than dietary nutrients that modifythe balance of microbial populationbypromoting the growth of beneficial bacteria and thereby maintain healthier intestinal environments.

4.    What is eubiotics?

Ans: Eubiotics are defined as non-antibiotic products maintaining the desiredbalance of good bacteriaand pathogens or eubiosis in the digestive tract. e.g. direct acting gut flora modulator (organic acid, essential oil), probiotics, prebiotics, immune modulator.

5.    What is supplement?

A. Answer the following questions

1.    Define feeding standards?

Ans: Feeding standards are the statements or quantitative descriptions of amounts of one or more nutrients needed by the animals or human.

2.    Limitation of feeding standard?

Ans: Palatability and physical nature of the ration are not included and does not included the environmental factors because environment may change the nutrient requirements.

3.    Write down the merits of ME system?

Ans: ME gives more correct result than DE or TDN. It is because energy losses in urine and GDP are corrected in ME system and ME is cheaper and easier to obtain than NE values.

4.    What is the purpose of following feeding standard?

A. Answer the following questions:

1.    What is NPN substances?

Ans: Feedstuffs which contain nitrogen in the form other than protein or peptides aretermed as non-protein nitrogen (NPN).

2.    What is liquid diet?

Ans: Liquid molasses containing 2-3% of uniformly mixed urea fortified with minerals andvitaminsis named as ‘liquiddiet’.

3.    Name few amides.

Ans. Urea (46.6% N) and Biuret (35%N)

4.    Name few amines:

Ans. Histamine from Histidine, Tryptamine from Tryptophan, Tyramine from Tyrosine, Betaine from Sugar beet pulp.

5.    Name few alkaloids.

A. Answer the following questions

1.    WhyvitaminAdeficiencyis far common in captive carnivores than free rangingwildlife?

Ans: Because they are fed exclusively on carcass meat without liver, cod liver oil or synthetic sources of vitamin A and moreover, thay can not convert ?-carotene to vitamin A.

2.    Why weekly off day feeding is practiced in tiger in all Indian zoos?

Ans: This ispracticed tostimulatetheintermittentfeedingbehavior of tiger in wild condition.

3.    Why metabolic bone diseases are very common in captive carnivores?

And: As the carcass meat provided in captivecondition is deficient in Ca and ratio of Ca: P (1:20) is very highly imbalanced.

4.    Write the extreme specializations in GItract seen in vampire bats?

Ans: Like blood sucking parasites, their GI tract is reduced to a tube since it feeds only on blood.

A. Multiple choices (you have to choose most appropriate answer among 4 alternatives)

1.    Which of the following bacteria in rumen is not cellulolytic

a. Bacteroides succinogenes

b. Ruminococcus flavefaciens

c. Ruminococcus albus

d. Succinivibrio dextrinosolvens

2.    Which of the following bacteria does not interact with anaerobic fungi

a. R. albus

b. R. flavifaciens

c. Butrivibrio fibrisolvens

d. Fibrobacter succinogenes

Livestock feeds have a variety of feed stuffs for feeding. Composition and characteristics differ even amongcloselyrelated feeds.Also, cost and availability of such feeds at a particular place, makes the farmer to choose for practical feeding. Therefore, it becomes imperative to learn about such classes. Feeds may generally be classified according to the amount of a specific nutrient they provide intheration. Theymayconvenientlybe divided intotwo general classes– roughages and concentrates. Roughages are bulky feeds containing more than 18 per cent crude fibre, but less than 60 per cent total digestible nutrients. Contrary to this, concentrates have less than 18 per cent fibre, but more than 60 per cent total digestible nutrients. Roughages may be further sub-divided intosucculent and drytypes.Whereas, concentratesare sub-dividedintoEnergy or basal feeds havingless than 20 percent crude protein and Protein supplements havingmorethan 20percent crude protein. Followingfeeds indicate theoutlines of classification [Flow chart 1] of the conventional feeds into broad categories with examples.

The bio-degradation process dates back to time immemorial when some wild ruminant animals were spotted feeding on the tree –woods in South Chile. The natives of this place called this feed as “Palo Podrido (Huempe)”, meaning – ‘animal feed’, as opined by F. Zadrazilof Germany. Later on, it was found that thiswood was actuallydegradedbycertaintypes offungi undernatural conditions and decomposed to Spent-wood, which turned out to be the animal feed. The scientists started working on these fungi as well as this type of process. Lately, the fungi were found to be belonging to higher basidiomycetes class, called: White-rot-fungi. They mainly degraded lignin of the wood, however cellulose and hemicelluloses to a limited extent only.

But, surprisingly it was found that the digestibility and crude protein content increased to a considerable extent. Later on, the scientists started working on spent-straws too. The cereal straws (lignocellulosics) constituted a staple-feed (basal) in the developingcountries for theseruminantanimals. But, thepresence of lignin (a biological plastic) limits the utilization of these cereal straws in the rumen eco-system. Thus, the concept of SolidState Fermentation (SSF) sparked into this type of investigation. Some examples of such white-rot-fungi were: Pleurotus ostreatus, Coprinus cineus, Sporotrichum thermophile, Phanerochaete chrysosporium, Polyporous berkeleyi, Neurospora crssa, Alternaria tenius to name a few. Some examples of substrates upon which such types of fungi grew for SSF were: wheat straw, paddy straw, paddy husks, ground nut shells, sugarcane bagasse, barleystraw, sunflower stalk, aspen wood, birch wood, saw dust etc. The resurgence of interest in the use of straw from cereal and grain crops as animal feed had been witnessed in early nineties to maintain the performance of animals. Following were some of the variations undertaken by different scientific groups:

1. Introduction

The term nanotechnology was first coined by Norio Taniguchi in 1974 to describe controlled thin film deposition (Taniguchi, 1974). Nanotechnology is now generally recognized to include materials with a size in at least one dimension in the 1-100 nm range, although this terminology is not strictly enforced (Rao, 2008). In food science, there are many types of naturally occurring particles that fall within this size range and the use of a strict definition is problematic due to the large distribution of sizes (Kruif, 1998). Many natural foods contain nanoscale components and their properties are determined by their structure. These have been eaten safely for generations. In fact, some of food’s most important raw materials (proteins, starches, and fats) undergo structural changes at the nanometre and micrometre scales during normal food processing(Morris and Parker 2008). Food proteins (for example, native beta-lactoglobulin, which is about 3. 6 nm in length) can undergo denaturation (via pressure, heat, pH, etc. ) 

1. Introduction

Jackfruit is the largest edible tree borne fruit in the world (Baliga et al., 2011). According to Haq (2006) India is considered as the birth place of jackfruit. The major jackfruit producing states in India are Assam, Tripura, Bihar, Uttar Pradesh, the foothills of the Himalayas and South Indian States of Kerala, Tamil Nadu and Karnataka. Jackfruit is a fruit prized in some areas of the world and allowed to go waste in others. According to Sidhu (2012) the extent to which jackfruit is being wasted in India is not exactly documented however, the wastage is approximately 75%. On an average a fully developed fruit may weigh 10-25 kg. Seeds occupy around 10 to 15% of the total weight of the jackfruit (Bobbio et al., 1978). It is reported that jackfruit seeds contain high amount of protein, fiber and carbohydrate (Ocloo et al., 2010). The seeds are viable for not more than three or four days (Sidhu, 2012). According to Abedin et al. (2012) the industrial utilization of jackfruit pulp are varied but the seeds are discarded from those food industries. Moreover, mature fresh seeds cannot remain fresh for long as they tend to germinate easily. So, converting jackfruit seed into flour is an option in order to increase the shelf- life of the seed and can substitute various cereal based flours for developing food products. Dumpling is a type of food which is made out of small pieces of dough wrapped around a filling that may include vegetables, meat, or other ingredients mixed together.

1. Introduction

With increase in consumer awareness and lifestyle the interest for health promoting natural food ingredients has expanded in recent years. However, globally it has become a great challenge to the manufacturers to ensure that such compounds should remain in their active form during processing and storage with an emphasis to its bioavailability profile. Oxidative stress is the major contributor to the pathogenesis of a number of chronic and degenerative diseases including atherosclerosis, Alzheimer’s and Parkinson’s disease, diabetes, DNA damage, carcinogenesis, cardiovascular and neuro degenerative diseases, allergies, premature ageing etc. (Gupta et al. , 2014; Svetli et al. , 2010). Endogenous antioxidant defense mechanisms are not always sufficient to fully scavenge the free radicals formed in the body during various processes. Therefore, dietary components having antioxidant properties, such as tea flavonoids, are very much necessary to supplement the activity of endogenous antioxidants. Tea is one of the most concentrated sources of flavonoids and phenolic acids which show antioxidant activity by sequestering metal ions and by scavenging reactive oxygen and nitrogen species (Frei & Higdon, 2003).

1. Introduction

Kiwi fruit (KF) is very much acceptable for preservation by freeze-drying (FD) technique, since it has very much health benefits such as low calorific value, low glycemic index (GI), more vitamins (particularly vitamin C), maximum load of potassium, presence of variety of pigments, few important enzymes, low allergic response (AR) and finally it is rich in natural antioxidants, polyphenols. It is translucent, green pulp and many black seeds arranged nicely with covered by hairy and brown skin. The KF seeds are small sizes and black colored surround inner circularly through the whole pulp. It gives a nice crunch during consumption and provides a number of essential nutrients. Therefore, it will be remembered that seeds cannot be discarded during processing of KF unlike other fruit (jamun etc. ) seeds.

1. Introduction

Underutilized fruits and vegetables are commercially neglected but recognized as rich sources of nutrients and non-nutrient bioactive compounds (Ali et al., 2008). These crops are very popular among local communities in many parts of the world and well accepted in their traditional diets and therapeutic medicines (Animesh et al., 2010). Nowadays, these are gaining significance in research and development programs of many organizations. Meghalaya is endowed with rich and unique diversity of indigenous wild edible fruit species of which most of them are less known and underutilized (Haque et al., 2009). There are rich in functional bioactive components such as vitamins and minerals are the much talked dietary nutrient for women and children health. It is being supplied to body through tablets, vegetables, fruits and other food items (Islam et al., 2012).

1. Introduction

The emergence of food borne illness due to contaminated food is one of the most frequently occurring event and challenge for the food processing industry. This has resulted into economy as well as energy requirement to counter the food borne pathogens. Some of the important pathogenic microorganisms of concern are E. coli, Listeria monocytogenes, salmonella, etc. To address the food safety and quality issues, several non-thermal food processing technologies have been developed in the last decades, with an aim of achieving food safety without significantly affecting the quality of the food product. Cold plasma in one of the non-thermal processing technology, which has shown promising results as evidenced by recent scientific investigations.

The plasma is known as fourth state of matter after solid, liquid and gas state. Plasma contains diversity of energetic species including ions, electrons, atoms, excited molecules and charged particles. In a plasma, a significant number of electrons have such a high energy that no nucleus can hold them. When a gas is subjected to heat or an electric field, some of the atoms become ions and the gas is said to be ionized. An ionized gas in its normal condition, can conduct electric current to a limited extent. If the heat or electric current becomes extreme, many of the atoms become ions. The resulting super ionized gas, which can conduct a large and sustained electric charge. Plasmas are used in several materials processing industries like textiles, electronics, paper and glass industries etc.

1. Introduction

Sundarbans is claimed to be one of the wonders of the world for its natural resources of halophytic mangrove forest (some 34 species of true mangroves are present), biodiversity and ecosystem that form an extraordinary environment of the globe. Available brackish water environment of Sundarbans provides favourable substratum for algal communities (Naskar and Naskar, 2010). Enteromorpha intestinalis specifically is one of the algal species that is very abundant in the Sundarban region. The production of total seaweeds in India in 2000 was approximately 600,000 tons (wet weight). India produces 110-132 tons of dry agar annually utilizing about 880-1100 tons of dry agarophytes. The seaweed industry in India is mainly a cottage industry and is based only on the natural stock of agar-yielding red seaweeds, such as Gelidiella acerosa and Gracilaria edulis, and algin yielding brown seaweeds species such as Sargassum and Tubineria (Kaladharan and Kaliaperumal, 1999).

Investigational studies indicate that marine algae contain significant amount of nutraceuticals, vitamins, proteins, carbohydrates, fiber and antioxidants. Composition indicates that there is a tremendous scope for food and pharmaceutical applications. These may be used for food fortification but after rigorous studies. Formulation, standarizaiton and characterization should be performed before human use. Studies for exploitation of the bioactive components of the species may open up new avenues. Hence there seems to be a full scope for the realization of outcomes of the research. Further the utilization of the developed processes at higher scales may reap in financial benefits and may prove beneficial to the local people, who are supplier of the raw materials. The present study is an attempt to utilize this India variety of macro-algae for fortification of bakery food products.

1. Introduction

Okara is a by-product generated during processing soybean into soymilk or tofu. Okara is rich in fibre and protein. It contains most of the carbohydrates, some of the protein and a small portion of the oil from soybeans. Okara contains approximately 50% fibre, 27% protein, and 10% fat on dry basis (Li et al., 2012). About 1. 2 kg of fresh okara is produced from every kilogram of soybean processed for soymilk (Vishwanathan et al., 2011). Fresh okara from soymilk production has about 80% moisture. The protein present in okara has good nutritional quality and superior protein efficiency ratio. Other soy components that are also likely present in okara include isoflavones (genistein and daidzein), lignans, phytosterols, coumestans, saponins, and phytates (Li et al., 2012). The most common method of utilization of okara is in the form of animal feed. Tofu or soymilk processing plants primarily dump okara as landfill or resort to burning. Okara can be utilized as a fertilizer as it contains various nutrients required for growth of plants. It can be used as a fermentation substrate for production of ethanol. Okara can also be used in pet foods as it contains high levels of extenders and protein. Apart from these, okara can be used in a variety of food products. It can be used in various food products. Utilization of okara is still in the initial stage. It can be used as a replacement of wheat flour in baked products. It can also be used in various soups, salads or vegetable preparations.

Resistant starch refers to the portion of starch and starch products that resist digestion as they pass through the gastrointestinal tract. Resistant starch is the fraction of starch which is not hydrolysed to D-glucose in the small intestine within 120 min of being consumed, but which is fermented in the colon. It can be used as a functional ingredient in various food formulations (Fuentes-Zaragoza et al., 2010). RS has desirable physicochemical properties (Fausto et al., 1997) such as swelling, viscosity increase, gel formation and water-binding capacity, making it useful in a variety of foods. It helps in improving the appearance, texture, and mouthfeel of the final product compared to conventional fibres. Resistant starch can act as a prebiotic for gut microflora (Sajilata et al., 2006). It has hypocholesterolemic effects, is hypoglycaemic and prevents gall stone formation.

1. Introduction

Organophosphate (OP) and organocarbamate (OC) pesticides are highly used in modern agriculture due to their low persistence and high insecticidal activity. Although they are relatively less persistent than the organochlorines, they have greater acute toxicity with tolerance level ranging from 1 to 8 μg mL-1 (Mulchandani et al., 2001). The high toxicity of OP and OC neurotoxins and their large use in modern agriculture practices has increased public concern, health risk and the consequent contamination of water and food sources (Buonasera et al., 2010). Development of fast and cost effective analytical tools for onsite monitoring of OP and OC contaminations in food, water and in the environment is highly necessary. An approach toward that has resulted the evolution of acetylcholinesterase (AChE) based biosensors for OP and OC pesticides. Ample literature is available on AChE based biosensors (Zhou et al., 2013). But some intrinsic disadvantages remain that limits its utility and field deployment. The most influencing ones are their inability to sustain in organic solvents and the limited class selectivity that demands alternate bioreceptor for pesticide detection. Given the diversity in mode of action of different classes of pesticide, it is quite challenging to come up with different sensor molecules for each class of pesticides. As a continuous effort towards that goal, attempts have been made to use the Glutathione S –transferses (GSTs; EC 2.5.1. 18) for the purpose. GSTs are a multigenic family of cytosolic proteins with multifunctional biological roles, widely distributed throughout the body and found in the liver, kidney, brain, pancreas, testis, heart, lung, small intestine, skeletal muscles, prostate and spleen (Jancova et al., 2010). The important biological functions those are making GSTs the focus of current research includes its catalytic role in detoxification reaction (Lumjuan et al., 2011) over expressions during phase II metabolism as well as drug resistance associated with anticancer therapies of human and the resistance acquired by certain insects while getting exposure to pesticides (Borah et al., 2016).

1. Introduction

The culinary banana (Musa ABB) of Assam and North East India (locally known as kachkal) is an important and cheap source of vegetable in the diet of local people. It is considered as one of the potential sources of carbohydrates, starch, polyphenols, micronutrients and functionally important bioactive compounds (Khawas et al., 2016). Native starches have limited use in food application as it has many weakness such as narrow peak viscosity range, poor process tolerance, low shear stress resistance, hydrophilicity, thermal decomposition, high retro gradation, and synersis and cooked starches will formed a weak, cohesive and rubbery paste. These facts motivated the employment of modified starches as important functional ingredients in processed foods in recent years because of their improved functional properties over unmodified starches (Fleche et al., 1985).

To overcome these problems physical. Chemical and enzymatic modification of Starch is done. Chemical modification of starch generally involves esterification, cross linking, etherification or oxidation of the available hydroxyl groups on the β-D-glucopyranosyl units that make up the starch polymer.

Introduction

Proteins are the most abundant organic compounds constituting major part of the body. Chemically, proteins are nitrogen containing macromolecules and polymers of L α-amino acids, which serve a wide range of structural and biological functions in animal body. For example, connective tissue made up of collagen, metabolicenzymes, neuro transmitters,signal in gmolecules, structural components of cell membranes and cell organelles, etc. all are chemically proteins.

Before goinginto the details of protein metabolism, first, we need to understand that there are majorly two sources of proteins in our body: dietary protein and body protein. Dietary protein is taken in diet, while body protein indicates the protein and amino acids synthesized in animal body to fulfill the requirements for growth and development. The digestion of proteins will not be discussed here as it has been described in detail in chapter 12. Dietary protein is digested in presence of several enzymes such as trypsin, pepsin, chymotrypsin, aminopeptidases and carboxypeptidases and is converted into mono-, di- and oligopeptides in stomach and small intestine; and finally converted into amino acids. These amino acids are transported to the systemthrough intestinal mucosa via active transport, from where they enter into the cells for further metabolism. Therefore, ‘metabolismof proteins’ is more appropriatelylearnt as ‘metabolism of amino acids’.

ABSTRACT

Agroforestry a sustainable land use system being practiced since long back through out the country. A diverse climatic, edaphic, and environmental conditions prevailing in the country has made it possible to practice different kinds of agroforestry systems. The local research organizations and individuals have undertook various studies for the betterment of the local agroforestry systems for getting higher yields to meet the need of food, fodder, fuel wood etc. It was found that Jowar+Teak+Papaya followed by Chilli+Teak+Papaya combination gave more economic returns compared to pure agricultural crop. Casuarina equisetifolia, Grevillea robusta, Dalbergia sissoo and Melia dubia have been found to be suitable for agroforestry systems in dryland. Coffee based agroforestry system and other tree-crop combination tried have been elaborated in this paper.

INTRODUCTION

Agroforestry is a new word for the old practice of growing woody plants with agricultural crops and/or livestock together on the same land. Various experts and scientists working on agroforestry or involved in land use related sciences have defined agroforestry according to their individual understanding of what they call agroforestry.

“Agroforestry is sustainable management system for land that increases total production, combines agricultural crops, tree crops and forest plants and or animals simultaneously or sequentially and applies management practices that are compatible with the cultural patterns of the local population” (Chundawat and Gautham, 1993).

Atoms and molecules are the essential building blocks of every object. The manner in which things are constructed with these basic units is vitally important to understand their properties and their reciprocal interactions. An efficient control of the synthetic pathways is essential during the preparation of nanobuilding blocks with different sizes and shapes that can lead to the creation of new devices and technologies with improved performances. Traditionally nanoparticles were produced only by physical and chemical methods. Some of the commonly used physical and chemical methods are ion sputtering, solvothermal synthesis, reduction and sol gel technique

Basically there are two approaches for nanoparticle synthesis namely the Bottom up approach and the Top down approach. In the Top down approach, scientists try to formulate nanoparticles using larger ones to direct their assembly. The Bottom up approach is a process that builds towards larger and more complex systems by starting at the molecular level and maintaining precise control of molecular structure.

These two different methods highlight the organization level of nanosystems as the crossing point hanging between the worlds of molecular objects and bulk materials.

The top-down approach has been advanced by Richard Feynman in his often-cited 1959 lecture stating that “there is plenty of room at the bottom” and it is ideal for obtaining structures with long-range order and for making connections with macroscopic world. Conversely, the bottom-up approach was pioneered by Jean-Marie Lehn (revealing that “there is plenty of room at the top”) and it is best suited for assembly and establishing short-range order at the nanoscale. The integration of the two techniques is expected to provide, atleast in principle, thewidest combination of tools for nanofabrication.

The most commonly used technique has been discussed and we have tried to generalize the type of particle that can be synthesized by each of these methods. Though it is not a rule that is applicable to all cases but it can be applied to most cases.

Polymerization Radical Polymerization

The polymerization could be a free radical polymerization or an atom transfer radical polymerization. The process of atom transfer radical polymerization (ATRP) is better than the free radical polymerization as control of molecular weight and size of the particle can be achieved. The coating of polymer on silica nanoparticles is generally done by ATRP. The surface of the silica particle is modified with a suitable initiator.

1. Mechanical Properties of Nanomaterials

Scientific challenges in nanoscience and nanotechnology include the development of nanomaterials with novel mechanical properties. The need for scratch, mar and/or abrasion resistance is well established in various markets, including fingernail polishes, flooring, plastic glazing, headlamp covers and other automotive parts, transportation windows and optical lenses, where clear scratch-resistant coatings are used. Because the nanosize, many of their mechanical properties of the materials is modified, among others, hardness and elastic modulus, fracture toughness, scratch resistance, fatigue strength, and hardness. Energy dissipation, mechanical coupling within arrays of components, and mechanical nonlinearities are influenced by structuring components at the nanometer scale. This includes also the interpretation of unusual mechanical behavior (e.g., strengths approaching the theoretical limit) and the exploration of new ways to integrate diverse classes of mechanically functional materials on the nano-size.

A Scanning electron microscope (SEM) is a type of electron microscope that images a sample by scanning it with a high-energy beam of electrons in a raster scan pattern. The electrons interact with the atoms that make up the sample producing signals that contain information about the sample’s surface topography, composition, and other properties such as electrical conductivity.

The types of signals produced by an SEM include secondary electrons, back-scattered electrons (BSE), characteristic X-rays, light (cathodoluminescence), specimen current and transmitted electrons. Secondary electron detectors are common in all SEMs, but it is rare that a singlemachine would havedetectors for allpossiblesignals. The signals result from interactions of the electron beam with atoms at or near the surface of the sample. In the most common or standard detection mode, secondary electron imaging or SEI, the SEM can produce very high-resolution images of a sample surface, revealing details less than 1 nm in size. Due to the very narrow electron beam, SEM micrographs have a large depth of field yielding a characteristic three dimensional appearance useful for understanding the surface structure of a sample.

Nanotechnology has the potential to revolutionizethe agricultural andfood industry with newtools forthemoleculartreatmentof diseases, rapid disease detection, enhancing the ability of plants to absorb nutrients etc. Smart sensors and smart delivery systems will help the agricultural industry combat viruses and other crop pathogens. In the near futurenanostructured catalysts will beavailable which will increase the efficiency of pesticides and herbicides, allowing lower doses to be used. Nanotechnology will also protect the environment indirectly through the use of alternative (renewable) energy supplies, and filters or catalysts to reduce pollution and clean-up existing pollutants.

Over the past few decades, the fields of science and engineering have been seeking to develop new and improved types of energy technologies that havethe capability of improvinglifeall over the world. In order to make the next leap forward from the current generation of technology, scientists and engineers have been developing Energy Applications of Nanotechnology. Nanotechnology, a new field in science, is any technology that contains components smaller than 100 nanometers. For scale, a single virus particle is about 100 nanometers in width.

An important subfield of nanotechnology related to energy is nanofabrication. Nanofabrication is the process of designing and creating devices on the nanoscale. Creating devices smaller than 100 nanometers opens many doors for the development of new ways to capture, store, and transfer energy. The inherent level of control that nanofabrication could give scientists and engineers would be critical in providing the capability of solving many of the problems that the world is facing today related to the current generation of energy technologies.

People in the fields of science and engineering have already begun developing ways of utilizing nanotechnology for the development of consumer products. Benefits already observed from the design of these products are an increased efficiency of lighting and heating, increased electrical storage capacity, and a decrease in the amount of pollution from the use of energy. Benefits such as these make the investment of capital in the research and development of nanotechnology a top priority.

Nanotoxicology is the study of the toxicity of nanomaterials. Because of quantum size effects and large surface area, nanomaterials have unique properties compared with their larger counterparts

Nanotoxicology is that branch of bionanoscience, which deals with the study and application of toxicity of nanomaterials. The nanomaterials, even when they are made of inert elements like gold, become very active at a nanometer range. Nanotoxicological studies are intended to determine whether and to what extent these may pose a threat to the environment and to human beings. For instance, Diesel nanoparticles have been found to damage the cardiovascular system in a mouse model.

Human health and safety

Calls for tighter regulation of nanotechnology have occurred alongside a growing debate related to the human health and safety risks associated with nanotechnology. The Royal Society identifies the potential for nanoparticles to penetrate the skin, and recommend that the use of nanoparticles in cosmetics be conditional upon a favorable assessment by the relevant European Commission safety advisory committee. Andrew Maynard also reports that ‘certain nanoparticles may move easily into sensitive lung tissues after inhalation, and cause damage that can lead to chronic breathing problems’.

Brooding and Rearing Practices Used for Chicken

What is Brooding?

Rearing of young ones immediately after hatching for at least 2-3 weeks, either by natural means or by artificial method is called brooding. Brooding is done by two ways i.e., Natural or Artificial ways.

Natural Brooding

In the natural method a desi fowl which acts both as incubator and brooder is used to raise only a small number of chicks. She sits on eggs (chicken) for 21 days to produce chicks from fertile eggs and broods thereafter.

Types of Brooders

Brooding is a mechanism of providing auxiliary or supplementary heat to chicks through artificial heat using brooders. Brooders are of several types depending upon the size and the nature of fuel used. These are individual, multiple units or central heating systems run by coal, wood, oil, gas or electricity. The essentialities of a good brooder are i) a dependable mechanism, ii) supply of fresh air, iii) dryness, iv) adequate space, v) easy for cleaning and disinfection, vi) protection against enemies, vii) safety from fire and viii) economy.

i) Hover brooders

Such brooders are more widely used in India wherever electricity is available. They maintain uniform temperature, convenient for operation and require very little attention. However, eclectic brooders do not heat house appreciably in very cold weather. Wet litter may be another problem requiring frequent stirring and renewal of litter.

Management of the poultry house environment forms an essential aspect towards maximizing production performance. Generally, proper maintenance of poultry litter is seldom given emphasis. The condition of litter significantly influences bird’s performance and ultimately affects the economy for rearing. The litter is defined as the bedding materials of around 5-6 cm height consisting of locally available materials like rice husk, peanut hull, wood shavings, paper shreds, sawdust or groundnut hulls. These materials can be used alone or in combination to rear the birds on floor under deep litter system of management. The excreta, feathers, spilled feed and water are mixed with bedding material, get further decomposed due to heat and bacterial action resulting in built-up-litter towards the end of rearing.

Any deviation from optimum state could be referred as stress. Every living creature has to pass through one or the other type of stresses in life. Most of the farm animals including poultry suffer from various types of stresses. These include due to:

Adverse environment

Managemental practices

Diseases

Nutritional deficiency

Transportation etc.

For maximization of productivity in any kind of poultry venture the knowledge on the behaviour of birds and its application play very significant role. Behaviour is the reaction of the whole organism to certain stimuli, or the manner in which it interacts to its environment. Behavioural patterns are not inherited as such, but develop through the process of growth and get differentiated under the influence of genetic and environmental factors. Primary function of behaviour is to enable the bird to adjust itself to some changes in condition, whether external or internal. Most animals have a behavioural pattern which can be tried out in a given situation and in this way they learn to apply one or the other according to that which produces the best adjustment.

Chicken’s eggs have been used as a food item by human beings since antiquity. The egg is composed largely of the proteins out of the three most important dietary essentials (proteins, fats and carbohydrates). The egg proteins are highly digestible and remarkably complete, containing the most important essential amino acids. The amino acid profile of egg is similar to the ideal balance of amino acids needed by humans. It also supplies various minerals, some in significant amounts, and contains a range of vitamins. The nutritive excellence of the egg enhances the value of any food in which it is incorporated. Meeting consumer demands is a constant challenge for the poultry industry. Present day consumers desire somewhat distinct products with respect to safety, healthfulness, freshness, taste, color, etc. To remain competent, various companies have developed several designer and specialty eggs which have appeared in supermarkets.

Artificial insemination (AI) is the deposition of the semen or male germ cells (spermatozoa) in the female genital tract by artificial means (instruments). Artificial insemination includes collection of semen from the male, preservation, handling and deposition into the female reproductive tract hygienically replacing natural mating by a male. In India this technique is gaining momentum in chickens due to improved animal husbandry practices and economic considerations, initiated by IVRI, Izatnagar and JNKVV, Jabalpur. In many parts of the world, turkey, ducks, goose and guinea fowl are bred solely by AI. Chicken species are endowed with the behaviour and capacity of each male to mate several females and also 25 to 40 times a day. Further, the process of ovulation in birds is almost daily and stimulated by intensity and duration of light.

In modern poultry managemental practices of using cages, environmentally controlled houses and breeding and hybridization techniques employed for developing highly economical chickens, selection for faster growth and heavy body weight in broilers, which has led to loss of fertility and hatchability and use of dwarf female parents in broiler industry have demanded the use of AI in poultry.

4.1. INTRODUCTION

The number of individuals to be included in a research study (the sample size of the study), is an important consideration in the design of many studies. This chapter will provide information on basic factors that determine an appropriate sample size and provides methods for its calculation in some simple, yet common, cases. Sample size is closely tied to statistical power, which is the ability of a study to enable detection of a statistically significant difference. A trade-off exists between a feasible sample size and adequate statistical power. Strategies for reducing the necessary sample size while maintaining a reasonable power is also important.

How many individuals will I need to study? This question is commonly asked by the clinical, ecological and behavioural investigator and exposes one of many issues that are best settled before actually carrying out a study. Consultation with a statistician is worthwhile in addressing many issues of study design, but a statistician is not always readily available.

The amount of information that can be gained from a sample depends upon its absolute size and not upon its size as a proportion of the population size, Just as to decide if the wine is good we need only a sip, irrespective of the bottle size, similarly we do not need to know the exact size of the parent population, provided complete homogeneity exists, But in reality, and more so in biological sciences, such homogeneity is rarely seen. Therefore, appropriate sample size becomes pertinent for drawing valid conclusions and is among the most difficult decisions to be taken for a study.

The size of the sample would depend upon (i) the permissible error in assessment of inference i.e. accuracy and (ii) sampling error. Sampling error measures the amount of variability between sample results; the lesser it is the better. The main determinant of the sample size, therefore, is how accurate results do we used. This in turn, depends on the purpose and type of study.

As homogeneity is a basic criterion, it is important to know the solubility of solutes, which is a temperature dependent property. Besides temperature, solubility is influenced by the acidity (pH) of the solvent due to the acid-base character of the solute. Bases tend to dissolve better in acidic solutions, whereas acids do in basic solutions. Therefore, one often needs to change the pH of solutions accordingly. As the most widely used concentration units (molarity, w/v% and v/v%) refer the amount of solute in a given volume of the solution, it is the volume of the complete solution that must be set to the desired (calculated) value thus, one must consider not only the solvent but the solute(s) as well. Another reason why one must add the solute(s) to an amount of solvent which is less than the final volume might be that one will need to set the pH subsequently in order to enhance solubility and/or to reach a desired pH value. The volume of acid or base solutions used for pH setting is usually not known in advance and is not negligible. If the solute was initially dissolved in the final desired volume, one would exceed this volume during pH setting (even if the volume of the solute is negligible) and, thus, the solution would not have the desired (calculated) concentration but a smaller (and mostly unknown) one. Thus, according to the correct procedure, one must take into consideration the fact that the final volume will contain the volume of the buffering component. value particularly in the case of dissolving proteins, which have a tendency to stick to the bottom of the dish, thereby making dissolution substantially slower.

There are other groups that contribute to the reactivity of enzymes beside amino acid residues.

These groups are called cofactors - chemicals required by apoenzymes (inactive) to become holoenzymes (active).

There are two types of cofactors:

1. Essential ions - metal ions -inorganic
2. Coenzymes - organic molecules that act as group-transfer reagents (accept or donate groups)- can also be H⁺ and/or e^-

Both provide reactive groups not found on amino acids side chains.

Coenzymes can be either cosubstrates (loosely bound to enzyme; is altered, then regenerated) or prosthetic groups (tightly bound to enzyme).

Coenzymes can be classified by their source:

NEW CELLS ARE PRODUCED BY DIVISION OF PREVIOUSLY EXISTING CELLS

All cells come from previously existing cells

To make a new cell identical to itself a cell must perform 4 tasks:

It must grow and make additional copies of all its organelles, enzymes, etc..

It must duplicate its DNA

It must accurately separate the DNA into 2 units, so that each cell gets a full set

It must divide into 2 cells (cytokinesis)

SEX IS VERY COSTLY

Large amounts of energy required to find a mate and do the mating: specialized structures and behavior required

Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.)

Genetic costs: in sex you pass on only half your genes to your children

Males are an expensive luxury- in most species they contribute little to rearing offspring

But There are Some Advantages

More genetic diversity: more potential for survival of species when environmental conditions change

Shuffling of genes in meiosis (random separation of homologous chromosomes)

Crossing-over in meiosis (see below)

Fertilization (genes from 2 individuals brought together)

A fair number of practical breeding experiments were done in ancient times

People knew there was a connection between sex and reproduction

The idea had developed that “like begets like”

By selecting certain plants and animals and breeding them many domestic varieties of plants and animals had been developed

Example: corn was developed from a wild grass in Mexico and Central America, probably about 5000 years ago

149 of the top 150 plant crops were domesticated by primitive man

BACTERIA HAVE SMALL, COMPACT GENOMES

We now know the complete DNA sequences for about a dozen bacteria

Bacteria seem to need about 500 to 5000 genes to conduct their lives

Because they are small, bacterial genomes are highly organized

Most of the time there are only a few bases between the end of one gene and the beginning of the next

Few introns are found in bacteria

Bacteria contain accessory “chromosomes” called plasmids; each has only a few genes

I. Definition

Solute transport in plants, translocation, primarily occurs in the phloem, but it can occur in the xylem.

II. Solute Transport in the Xylem

Some solutes are transported in the xylem

Water and dissolved ions are the main substances in vessels/tracheids

These materials are transported via transpiration stream

Xylem sap may also contain organic materials, usually in relatively low concentration (with a notable exception being maple sap in the spring which is comprised of 2% or more sucrose).

Substances move at different rates depending on matrix effects, metabolic needs, etc.

PLANT NUTRITION

Plants use inorganic minerals for nutrition, whether grown in the field or in a container. Complex interactions involving weathering of rock minerals, decaying organic matter, animals, and microbes take place to form inorganic minerals in soil. Roots absorb mineral nutrients as ions in soil water. Many factors influence nutrient uptake for plants. Ions can be readily available to roots or could be “tied up” by other elements or the soil itself. Soil too high in pH (alkaline) or too low (acid) makes minerals unavailable to plants.

INTRODUCTION

The weather conditions during the growing season of crops influences the onset and development of disease. The relationship between weather and plant disease development is the basis for meteopathological forecasting. On the basis of observed or forecast weather conditions, the incidence of plant disease is likely to be predicted.

Such predictions are designed to warn growers of expected significant developments in the manifestation of a particular plant disease. Such disease forecasts, if sufficiently accurate, permit efficient and competent counter measures to be adopted. A short range or medium range of disease forecast, issued at short intervals during the growing of crops, can give guidance to the farmers on the best remedial measures. Growers can decide whether a chemical control measure is needed or not, and if needed then optimum date on which to be applied and similar day to day decisions to fight against disease to minimize the losses to be caused.

The result, of adopting such a plan of weather based control measures, is to reduce the number of chemical sprays to a well timed few, thus giving an equal or even better protection at a lower cost in chemical and labour, and simultaneously reduces losses caused by phytotoxic and mechanical damage to the crop. The reduction in the use of potentially toxic chemical is a matter to be welcomed in the present world, considering the consequences of the widespread use of toxic chemicals.