eChapters

Preamble

In India, agriculture plays an important role as it accounts for about 18% of the GDP and supports nearly 58% of the total working population for employment. Food grain production increased significantly from a low level of 50 million Tons during 1950-51 to a record of 230.7 million Tons in 2007-08. The demand for food is growing rapidly due to increasing population and rising income levels, hence we need to produce about 320 million Tons of foodgrains by the year 2025. It implies more pressure on our exhisting land, soil and water resources which are already in short supply, degraded, fragile and this similar situation is being faced by mainly developing countries today. Plant parasitic nematodes are of great economic significance. Since most of them live in the soil hence represent one of the most difficult pest problems in terms of identification, demonstration and management.

Barleria L. (Acanthaceae) is a pantropical genus of herbs and shrubs comprising about 300 species, occurring mainly in Africa and Asia. In India, the genus is represented by about 32 species of which 24 are endemic to India.  About 80 % of the species are found in Peninsular India. Many species of Barleria are of ornamental value and can be grown for their foliage and showy colorful flowers. There are certain indigenous species which show good growth performance in cultivation and have economic importance as ornamentals. Moreover, some species fall under IUCN categories of threatened plants which need immediate attention for their conservation. Considering the horticultural importance especially in landscape gardening and rarity of Barlerias, various species found in India are collected and cultivated through various methods in the Botanical Garden of Shivaji University, Kolhapur.

India produces all deciduous fruits including pome fruits (apple and pear) and stone fruits (peach, plum, apricot and cherry) in considerable quantity. These are mainly grown in the North-Western Indian States of Jammu and Kashmir (J&K), Himachal Pradesh (H.P.), Uttarakhand and in Uttar Pradesh. The North-Eastern Hills region, comprising of the States of Arunachal Pradesh, Nagaland, Meghalaya, Manipur and Sikkim also grow some of the deciduous fruits on a limited scale. The sub tropical region lies between 150 and 300 north of equator covering North Indian plains, the gangetic basin, large parts of North eastern state, western ghats, nilgiri regions etc. In this zone spring is generally frost free and chilling availability ranges from 50-500 hours. This region is not considered proper for temperate fruit growing but grows several low chilling varieties of peach, plum, pear etc. Deciduous fruits, such as peach and nectarine, require accumulated exposure to cool temperatures during winter dormancy for bud break and the resumption of normal growth the following spring. Minimum amount of accumulated cool temperature exposure needed for normal growth, which varies by cultivars, is referred to as the chilling requirement. Due to introduction and adaptation of low chilling cultivars of peach, plum and pear, they are also now being grown commercially in certain areas of the north Indian plains.

Chironji or Charoli (Buchanania lanzan Spreng Family: Anacardiaceae) is an excellent tree of agro forestry and social forestry. In wasteland development and Dryland horticulture, it assumes great significance due to multifarious uses and capacity to withstand adverse climatic conditions. At present, it is growing under forest condition as an under exploited fruit and gives monitory reward to tribal community of country and seems to be a boon for them. It is a valuable species found in dry deciduous forest throughout the country excluding eastern Himalayan forests (Singh, 1982). Flesh of ripe fruit is very palatable and is largely eaten raw or roasted and oily kernels are the most important part and are used in preparation of sweet meats and puddings (Singh, 1982). Mesocarp of fruit is edible and cherished by children (Munde et al, 2003). Kernel is highly nutritious and rich in protein (18.00 %) and yields sweet oil, which can be used to substitute olive and almond oil. Kernel contains 33.50 % oil, 1.90 % of which is unsaponifiable. Saponifiable part contained 20.00 % of linoleic acid. Chironji oil is non-repellant and non-toxic and is suitable for human consumption (Banerjee and Jain, 1988). Hemawathy and Prabhakar (1988) reported that total kernel lipid extracted from chironji was 65.60 % of dry kernel and consisted of 90.40 % neutral lipids, 3.4 % glycolipids and 6.2 % phospholipids. Kernel can be used for preparation of sweets. It provides quality timber wood for various uses.

The extensive use of improved types of vegetable crops has led to a  situation that the indigenous types are virtually disappearing from ecosystems creating a vacuum in crop biodiversity. Biodiversity of genes, species, ecosystems and habitats will remain under threat. Habitats will be decreased and fragmented, endangering many indigenous, rare, endemic and specialist species populations and ecosystem functions, although generalist and invasive species will continue to spread. Therefore, loss of biodiversity can be considered at three scales: genes, species or populations and habitats or ecosystems. Maintaining thriving natural systems is essential not only for economic or ethical reasons, but also for ecological, social, recreational, educational and aesthetic reasons (Gomez, 1996). Most of these are easy to grow, resistant to pests and diseases, hardy and acceptable to local taste. They are rich in carbohydrate, proteins, fats, vitamins and minerals; hence the diets could be diversified to meet the nutritional requirements of the people in the country. The lack of micronutrients, including certain vitamins, minerals and other components needed for a healthy diet, is a pervasive and growing threat throughout the world.

Palms are monocot species, belonging to the family Palmae (Arecaceae). They are perennial and distinguished by having woody stems. Coconut, Arecanut and Oil palm are the major crops coming under this group. Palmyra is a naturally occurring palm in many parts of Tamil Nadu, Andhra Pradesh, Karnataka, Kerala, Orissa, Bihar and West Bengal States. Palmyrah is utilized by the locals for tapping inflorescence sap and for fruit. Palms of India are represented by 20 genera and about 96 species among which 24 species belonging to nine genera are endemic to India. The largest genus, Calamus with 44 species is followed by Phoenix seven, Pinanga five and the rest with less than five species each. The genera Borassus, Salaca, Livistona, Nypa, Hyphaene and Ptychoraphis are represented by a single species each. In addition, C. nucifera and A. catechu are extensively cultivated as plantation crops. They have almost naturalized in parts of Kerala and Andaman Nicobar Islands. Except P. sylvestris, C. urens and B. flabellifer which are widespread in peninsular India, the rest are of restricted distribution in specialized eco-geographical regions of the country. The forests of Andaman–Nicobar islands (13 genera, 24 species), northeastern and eastern Himalayan region (12 genera, 36 species) and the Western Ghats (7 genera, 30 species) are major centres of palm concentration. Nypa fruticans and Phoenix paludosa are fringe mangrove species occurring in salt marshes of Sundar-bans and Andaman–Nicobar islands. P. paludosa is also found in Orissa.

Wild fruits were an important source of food for mankind before dawn of civilization and domestication of present day fruits. Cavemen in forests also depended on these fruits and passed on valuable information on utility and choice of wild species of fruits from generation to generation.Thus the present day horticulture came into existence. Also, wild varieties of plants, yielding edible fruits growing throughout the Himalayas, contributed directly to cultural heritage of India. Even today, these fruits are eaten in plenty by local people, as they are commonly available in abundance in their habitats. Fruit crops, which are neither grown commercially on large scale nor traded widely are called underutilized fruit crops. These are cultivated, traded and consumed locally. Underutilized fruit crops have many advantages in terms of easiness to grow, hardy in nature and  production of good crop even under adverse conditions. Most of them are rich sources of vitamins, minerals and other nutrients. Underutilized fruit crops play crucial role in successful running of processing industry round the year in the region. A large proportion of rural population depends on locally available fruits to meet their dietary requirements

Cleome gynandra is one of the most underutilised crops in the world. It is the best known by rural and urban communities of Africa. The plant is well distributed in parts of South Africa, C. gynandra is indigenous to the country and evenly spread throughout Africa. Many people use it in their daily diets but it is not yet found on formal commercial markets. It is still regarded as a weed by commercial farmers. The nutritional value of the leaves is totally underestimated. The plant is known for the treatment of various ailments originated from epilepsy to headaches. The crop is harvested mainly from the wild and only cultivated in certain areas. The germination of the seeds can be a problem especially if the seeds are harvested simultaneously. The seeds must be harvested as it gets matured not all at once. Most consumers harvest the plant as needed. The more the leaves are picked the more growth is stimulated. A general harvesting method is not worked out yet, as this will differ from area to area.

Steps in the Estimation of Trace Elements
Estimation of trace elements in feeds and fodders and other biological materials involves the following steps :

    1.    Production of an acidic solution of the inorganic elements in the biological samples after removal of the organic matter by dry ashing or wet oxidation.

    2.    Removal or masking of interfering substances.

    3.    Determination of the selected elements, usually by using colorimetry.

Results of trace elements determinations are expressed as parts per million (ppm), mg/kg or µg/g dry matter.

INTRODUCTION

Vegetables and fruits have many similarities with respect to their composition, methods of cultivation and harvesting, storage properties and processing. In fact many vegetables are considered fruits in the true botanical sense. But the difference between the two is that vegetables are derived from various parts of plants e.g. roots, leaves, stems, buds and so on whereas, fruits are the mature ovaries of plants with their seeds. The edible position of most fruits is the freshly part of the pericap or vessel surrounding the seeds. The other difference between the two is that those plant items that are generally eaten with the main course of a meal are often considered to be vegetables, whereas those that are eaten alone or as a dessert are considered as fruits. This is the distinction made by food processors, certain marketing laws, and the consuming publics.

Sir Alexander Ogston (1880) first isolated Micrococci in pus and in 1883 he subdivided them into two groups i.e. Staphylococcus and Streptococcus. He also first proposed the name ‘Staphylococcus’ (Greek ‘staphyle’ means bunch of grapes and ‘kokkos’ means berry). Experiments of Bumm (1885), Garre (1885) and Bockhart (1887) established its relationship with human infections. As well as Nocard (1887) first isolated Staphylococcus from mastitis in sheep and found its pathogenic significance in animals.

Properties of Genus

Morphology : Staphylococci are gram positive cocci, capsulated, non-sporing and non-motile. Each coccus cell is 1µm in diameter.  They are arranged in a group or grape like cluster as cell division occurs in more than one plane. During preparation of smear and staining the cluster is sometimes broken and cocci are seen in pair or single (Fig. 1).

Staphylococcal capsule is polysaccharide in nature. The capsule can inhibit phagocytosis and helps in virulence.

 Staphylococcal cell wall contains three major components i.e. peptidoglycan (PG), teichoic acid (TA) and surface proteins.

Lucke (1862) first described that the bluish green discolouration of surgical dressing is associated with the presence of small vibrio like organisms. Latter Gessard first isolated the organism as Pseudomonas in 1882.

Properties of Genus

1. Morphology : They are Gram negative straight or curved rod, motile by one or more polar flagella. Usually it is 1.5 µm in length and 0.5 µm in width. Longer filamentous forms are sometimes observed. The bacteria are non- acid fast, non- sporulating and uncapsulated. Pili on the bacterial surface are detected. Environmental and strains present within tissue can produce slime. The slime is composed of glycolipoprotein (GLP) and polysaccharide of LPS.
    
2. They are strict aerobe.

Classification : Pseudomonas belongs to class Gamma-Proteobacteria, order- Pseudomonadales, family- Pseudomonadaceae.  Previously Pseudomonas included two group of bacteria viz. Fluorescent group and Pseudomallei group. Recent classification system included this Pseudomallei group into a new genus known as Burkholderia. Important species of Pseudomonas are:

SUMMARY

Fly ash, a waste product generated by coal based thermal power plants. The annual production of fly ash in the country has increased from 40 million tones in 1994 to 110 million tones in 2004 and it is expected that by 2012 the annual production of fly would reach the mark of 170 million tones. Owing to its physico-chemical characteristics, it has a vast potential for use in agriculture, forestry and wasteland reclamation. Application of fly ash into soil has been reported to increase the water holding capacity, hydraulic conductivity, porosity and decrease bulk density, modulus of rupture and surface encrustation. The studies conducted by various organizations on fly ash use in different crops have revealed significant increase in the crop yields such as wheat, maize, paddy, soybean, sunflower and vegetables like brinjal, tomato, potato, cabbage. Application of fly ash has been found to increase the level of heavy metals  viz., Co, Pb, Mo, As and Se as well as the activity of radio-nuclides i.e., ⁴⁰K, ²²⁶Ra, ²²⁸Ac  in the soil- plant system; however, the levels of all these heavy metals and radio-nuclides were found to be well below the permissible limits.

INTRODUCTION

The fine feeder roots of many vascular plants are invaded by specific nonpathogenic fungi forming a distinct morphological unit known as mycorrhiza (In Greek, Mykes mean mushroom or fungus, rhiza mean root). Physiologically mycorrhiza represents a case of symbiosis.

The term mycorrhizae were coined by a German botanist Albert Bernard Frank in 1885 which literally mean “fungus-roots”.  Mycorrhizal fungi form a symbiotic association with agricultural and horticultural crops in addition to certain tropical tree species.
Mycorrhizal fungi are broadly classified in to two groups:
1) Ectomycorrhizae  
2) Endomycorrhizae.

INTRODUCTION

Soil-borne pathogens cause heavy losses to most agricultural crops. Repeated planting of a crop in the same piece of land results in a high inoculum build-up, which forces the farmers either to change the crop or the land. Thus, the search for new, effective, inexpensive and non-hazardous methods for the control of soil-borne diseases is a continuous one. Over the years, efforts have been made to manage the soil-borne pathogens by employing fumigants, chemical pesticides, biological control agents, different type of soil amendments and also by causing modifications in different cultural practices. But, pathogen control is often hampered by the fact that both the inoculum of the pathogens and the lethal agents applied to the soil are affected by the soil’s physical, chemical and biological environment. Keeping in view the bottlenecks with different methods of management of soil-borne pathogens; harnessing of solar energy i.e. soil solarization is an effective method to control soil-borne pathogens.

1. Introduction

Pectin is a polysaccharide present in the middle lamella of plant cell walls. The firmness and structure of the plant tissue are attributed to the presence of pectin in the middle lamella. The insoluble form of pectin is called protopectin and the soluble form is called soluble pectin (Kertez, 1951). Protopectin is abundant in green fruits and contributes to hardness of cell, where as soluble pectin is responsible for viscous nature of juice and pulp. Conversion of protopectin to soluble pectin results in loosening of the bonds between the cells and softening of the fruit tissues. Protopectin is reported to be hydrolyzed to pectin by enzyme action during ripening. Protopectin on restricted hydrolysis yields pectin or pectinic acid. Braconnot (1825) discovered pectin as the gelatinous principle of fruits.

Genotypes × Season interaction is of major importance to the plant breeder in developing stable high yielding varieties of crop plants. When new varieties are tested over a series of seasons, the relative ranking of the varieties for any given attribute is rarely the same at each season. This increases the difficulty of identifying superior stable genotypes. Comstock and Moll (1963) have shown statistically the effect of large genotype × environment interaction is reducing the progress from selection.

In order to minimize the genotype × season interaction, stratification of the seasons can be practiced. The implification in stratification is that suitable selections could be made for the infavourable and favourable seasons encountered within the sampling units proposed. Stratification of environments has been practicised for a long time to identify suitable selection that could be made for the narrower range of environments encountered within the sampling units proposed. However, even with this refinement of technique, the interactions of genotypes with locations in a sub-region and within environments encountered at the same location in different years, frequently remain too large (Allard and Bradshaw, 1964). Little is known about the environmental factors contributing to such interactions. Sprague (1966) suggested that even if such information are available, the possibility of materially reducing genotype × environment interaction in field experiments would remain questionable.

On line with Allard and Bradshaw’s (1964) statement, the interactions of genotypes with locations in a sub-region with different seasons at the same location in different years, may remain too large. As far as sesame is concerned, practically nothing is known about the factors contributing to such genotype × season interactions. Hence, the progress in breeding a stable variety of sesame has been slow. Since sesame in India is grown essentially as rainfed crop, fluctuations in yield levels closely follow changes in the environment and seasons. Breeding for multiseasonal variety for may small regions within a state is being considered necessary. There is widespread awareness of achieving stability of production as well as improvement in yield of sesame under low and high-in-put conditions as well as for different seasons.

INTRODUCTION

Hybrid crops are derived from an established and well-proven breeding method used in ornamental sectors. The development of hybrid crops has been one of the major factors behind a dramatic increase in global crop yields. Hybrid is produced by crossing between two genetically dissimilar parents. Pollen from male parent called pollen parent will pollinate, fertilize and set seeds in female called seed parent to produce F₁ hybrid seeds. For production of a hybrid, crossing between two parents is important, the crossing process will results in heterosis or hybrid vigour. Heterosis is defined as the increase in size or vigour of a hybrid over its parent. This boost in performance, combining the best yield, quality and agronomic characters from each parent, is the result of hybrid breeding.

F₁ hybrid seed offers to the growers a significant benefits in terms of yield improvement, agronomic performance and consistency of end-use quality. But attempting to farm-save hybrid varieties will result in segregating populations producing highly variable offspring, which can lead to reduced yield, disease resistance and quality. As this factsheet explains, it doesn’t make economic or agronomic sense to save seed of an F₁ hybrid. In self pollinated crops it is difficult to cross but in cross pollinated crops it is easier. In nature to create genetic variability and for its wider adaptation in different environmental conditions, flowering plants has adopted many mechanisms for cross pollination. Cross pollination results in genetic heterogeneity and show wider adaptations. F₁ hybrids are in immense usage throughout the world with the rapid increasing interest in flowers, however the total number of the hybrids developed in flowers is still less. In India hybrid seed production is taken up in Bengaluru, Pune, Delhi, Punjab, Haryana, Jammu and Kashmir and Himachal Pradesh for export.

F₁ hybrids in ornamental crops were known even before Shull 1911 when he propounded the classical theory of hybrid vigour in plant breeding for the first time. In 1909 F₁ hybrid variety Prima Donna in begonia was released by Benary Seed Company in Germany. The first commercial F₁ hybrid in petunia was produced by Japan during World War II, in 1942. From 1950 onwards, F₁ hybrids were available in ornamental flowers from The Netherlands, Denmark, Germany, USA and UK besides Japan and produced in flower crops like ageratum, anemone, gerbera, primula, petunia, cyclamen, marigold, pansies, begonia, geranium, portulaca, dianthus, balsam, stock, wall flower, ornamental sunflower, gazania, hollyhock, calceolaria and zinnia. Flower breeders became familiar with the F₁ hybrid production of those crops also which were formerly propagated by vegetative parts such as pelargonium and gerbera. A few important hybrids were first released in petunia in 1940-50, geranium single in 1960, antirrhinum, marigold, pansy and zinnia in 1965, ageratum in 1966, geranium double in 1970, dianthus, impatiens, portulaca in 1976-77, gerbera in 1980 and carnation in 1981.

Tuberose (Polianthes tuberosa L.) belonging to family Amaryllidaceae is half hardy, perennial, bulbous plant. Bulbs are made of scales and leaf bases and stem remains concealed within scales. The generic name Polianthes is derived from Greek word “polios” meaning shining or white and “anthos” meaning flower, in allusion to the blooms of the common tuberose and species tuberosa, the plant being tuberous in nature. Tuberose is commercially cultivated in tropical and sub tropical areas for cut and loose flower trade and also for the extraction of it’s highly valued natural flower oil.

ORIGIN AND DISTRIBUTION

Tuberose is a native of Mexico (Bailey, 1976) from where it spread to different parts of world during 16^th century. The name tuberose is derived from tuberosa, this plant being the tuberous hyacinth as distinguished from the bulbous hyacinth. The name therefore, is tuber-ose, not tuberose. By the middle of the 18^th century the tuberose was still referred for as Hyacinthus indicus tuberosus. Originally Linnaeus had used the name Polianthes floribus alternis in his Hortus Cliffortianus. Later it was grouped under the genus Polianthes and the species tuberosa.

Tuberose is cultivated on large scale in China, Egypt, France, Italy, Israel, South Africa, New Zealand, Spain, Sri Lanka, North Carolina, USA and in many tropical and subtropical countries.

In India tuberose is commonly known as Gulchari and Gulshabbo in Hindi, Rajanigandha in Bengali, Sukandaraji and Nelasampengi in Telegu, Nilasampangi in Tamil and as Sugandharaja in Kannada.

In India its commercial cultivations is confined to Bagnen, Midnapur, Krishnanagar, Ranaghat, Kolaghat and Pariskura in West Bengal, Guwahati and Jorhat in Assam, Pune, Thane, Sangli, Nasik and Ahmednagar in Maharastra, Udaipur, Ajmer and Jaipur in Rajasthan, Navsari and Valsad in Gujrat, Ambala, Gurgaon and Faridabad in Haryana, Ludhiana and Patiala in Punjab, Devanahalli, Tumkur, Kolar, Belgaum and Mysore in Karnataka, East Godavari, Guntur, Chitoor and Krishna district in Andhra Pradesh and Coimbatore and Madurai in Tamil Nadu. Growers of Uttar Pradesh, Delhi and Orissa are also concentrating in tuberose cultivation after evolvement of improved varieties namely Shringar, Suvasini, Prajwal and Vaibhav by Indian breeders (Singh, 2006).

Ixora is an ornamental plant which is a medium-sized, tropical flowering, evergreen shrub that is used in landscapes. The genus Ixora belongs to the family Rubiaceae. The plants have glossy green leaves which are arranged oppositely along each stem. All Ixoras produce clusters of star-shaped flowers on stem tips or terminal growth. They are grown for their beautiful flowers which bloom in summer in clusters of different shades of red, pink or yellow (De Block, 1998 and Husain and Paul, 1988). The colour of the wild flowers is red or reddish-orange but ornamental varieties may have white, yellow, salmon or pink flowers. It is commonly known as West Indian Jasmine. The other common names of this plant are Jungle Geranium, Flame of the Woods, Jungle Flame, Burning Love, Red Ixora, Ixora, Amor Ardiente, Cruz de Malta, Dola de Coral, Equisosea and Rajana (Griffiths,1994 and Liogier,1997), Rangan, Kheme, Ponna, Chann Tanea, Techi, Pan, Santan, Jarum-Jarum and many more. Members of Ixora prefer acidic soil and are suitable for bonsai (Frommer, 2001). I. coccinea is used extensively for landscaping and is also grown as a potted ornamental (Lakshmanan et al., 1997). Ixora coccinea and Ixora parviflora are generally used as the ornamental plants in gardens and parks.

It is also a popular choice for hedges in parts of South East Asia like Thailand. Red Ixora flowers are commonly used in Hindu worship as well as in Indian folk medicine. The flowers, leaves, roots and the stems are used to treat various ailments in the Indian traditional system of medicine, the Ayurveda. The fruits are used as a dietary source. The plant possesses antioxidative, antibacterial, gastroprotective, hepatoprotective, antidiarrhoeal, antinociceptive, antimutagenic, antineoplastic and chemopreventive effects. The oil extracted from the flowers is helpful in conditions like scabies, eczema, pruritis and other skin infections. It is also used for hedges and borders and planted as an indoor potted plant. In laboratory tests, extracts of Jungle Geranium have shown anti-bacterial (Kumer et al., 1997) and anti-tumour (Serrame and Lim-Sylianco, 1995 and Latha and Panikkar, 2000) activity. From a methanol extract of the flowers, 13 chemicals were identified including ursolic acid which has known antitumor and antiviral activity (Monteath et al., 2001). In the traditional medicine of India, infusions of the leaves and juice from the roots are used to treat a wide variety of ailments including dysentery, ulcers, and gonorrhea (Parrotta, 2001).

Pansy belongs to the family Violaceae, genus Viola and species tricolor. Pansy also known as butterfly flower, cat face. Pansies are annuals or short-lived perennials, grow 15-30 cm tall and have heart-shaped leaves at the base and oblong leaves growing from the stems. The pansy is quite durable and a “flower for all seasons”. The fragrant and edible blooms are desirable in gardens. Pansy popularity increases possible due to its ease of growing. Whether grown from seed or bedding plants, pansies are relatively disease and pest free blooming prolifically for the gardener’s enjoyment. The pansy is a group of hybrid plants cultivated as garden flowers. Pansies are derived from Viola species Viola tricolor hybridized with other Viola species, these hybrids are referred to as Viola × wittrockiana or less commonly Viola tricolor hortensis. The pansy is used in phytotherapy. The pansy is the symbol of Free thought. For Viola species, there is strong resistance to cold, flowering early and long flowering period. Also be used to cover a large area on the ground flower beds, its colour range, forming a unique landscape in early spring, but also in the flower bed with the string of red, petunia colour, or for potted plants and planted tank, decorative balconies, bay windows and stairs.

ORIGIN AND DISTRIBUTION

The modern pansy, Viola x wittrockiana, is thought to have derived from Viola tricolor, a native of central Europe. The center of origin for Viola was continental Europe. Viola is widely distributed in temperate areas in the northern hemisphere and in S. America, with centres of diversity in S.E. Europe, E. Asia and S. America; isolated endemics occur in Africa (equatorial high mountains and the Cape), E. Australia, Tasmania, New Zealand and Hawaii. V. sororia as defined by McKinney (1992), is widely found across the United States and Canada. Each subspecies has a smaller distribution. V. sororia var. sororia is found in the mid to eastern United States and southern Canada, V. sororia var. affinis, is found in western and extreme northeastern sections of the United States and Canada, V. sororia var. missouriensis is found in the eastern United States, and V. sororia var. novae-angliae is found in Minnesota, Wisconsin, Maine, Quebec and New Brunswick. Of the four varieties only V. sororia var. sororia and V. sororia var. missouriensis are native to New Jersey (McKinney, 1992). V. sororia var. sororia is found in drier areas than the other four subspecies and often in waste grounds (McKinney, 1992) or on the forest floors of open woods in semi-shaded conditions (McKinney, 1992).

Salvia belongs to family Lamiaceae. The genus Salvia comprises about 900 species which are grown for their ornamental flowers since they make excellent and showy borders in gardens. One of the species most commonly grown is Salvia splendens is a tender herbaceous perennial, but grown in India as winter season annual flowering plant. It bears racemes of bright scarlet flowers of large size. It has ability to grow under partial shade condition. It is commonly grown as bedding plant due to varying colours mostly scarlet, purple, orange, lavender and yellow and long lasting flowers. The word salvia was derived from Latin word ‘Salvare’ meaning ‘to heal or to be safe and unharmed’ (Blumenthal et al., 2000 and Kamatou et al., 2008) later it was translated in French as “sauge” or “sage” and in old English “Sawge” (Kamatou et al., 2008). Salvia has several ornamental, culinary and medicinal species (Codd, 1985, Paton, 1991, Gali-Muhtasib et al., 2000, Kamatou et al., 2008 and Bruna et al., 2006). It is also used in preservation of food, mainly meat and cheese, due to its antioxidant properties, as well a spice for flavouring food articles (Topuc, 2006). Chinese use its species to increase longivity and Roman use it in ceremonies as scared herb (Kamatou et al., 2008). The salvia species contain secondary metabolite the main are terpenoids and flavonoids and monoterpenes present in aerial parts of plant like flower and leaves and diterpenoids mostly in roots (Topuc, 2006).

ORIGIN AND DISTRIBUTION

The centre of origin of salvia is considered to be Afghanistan and Soviet Central Asia as a large number of primitive morphological species are present but the most of the diversity or species are present in Mexico (Paton, 1991 and Kamatou et al., 2008). The genus is sub-cosmopolitantly distributed worldwide, but is absent in the North and most of the low-lying tropical areas of the world such as the Amazon basin and central and west Africa (Paton, 1991), Jager and Van Staden (2000) and Kamatou et al. (2008) reported its absence not only in western Africa but also in central tropical Africa, Hedge (1974) and Kamatou et al. (2008) reported that West and the southern parts of Africa has greatest number of species (Kamatou et al., 2008).

The genus Salvia contains at least 900 species, because they readily cross pollinate, innumerable hybrids - both natural and manmade. Salvias are found on almost every continent in the world. Pliny the Elder, a Roman naturalist, wrote about their healing qualities back in the first century. In the early 17^th century, the English botanist John Gerard, in his famous Herbal, described a number of sages, including common garden sage and one that sounds similar to what we now call ‘Tricolor’ sage. He referred to the healing powers of these herbs as well. Until the 19^th century, most gardeners focused on growing plants for medicinal or culinary purposes; beauty was a secondary, much less important consideration.

The results of plant exploration in the 18^th and 19^th centuries brought scores of new salvias to English and European gardeners from Mexico, China and Africa. One, Salvia buchananii, was found in Mexico by an English woman, who gave it to a man serving in the army. Salvia coccinea, indigenous to Mexico and South America, was grown for decades as a wildflower. Both scarlet sage and mealy cup sage were discovered in the early 1800’s, the former in Brazil, the latter in Texas.

Geranium (Pelargonium spp.) belongs to the family Geraniaceae. The name Pelargonium was introduced by Johannes Burman a Dutch Botanist in 1738, from the Greek word “Pelargos” means stork because the seed head looks like a stork’s beak. Due to their long and beak-shaped fruits, they are also known by the name of cranesbills. Geraniums are popular flowering plants and grown as decorative pot plants for indoor and outdoor. There is a world demand for the rose geranium essential oil of Pelargonium species which contains geraniol, linalool and citronellol, substitutes for the expensive attar of roses in the perfume trade. Pelargonium species was known for their medicinal value by the earliest indigenous people (Swanepoel, 2009).

ORIGIN AND DISTRIBUTION

The plants originated from South Africa (Swanepoel, 2009), as well as Reunion, Madagascar, Egypt and Morocco and were introduced to European countries such as Italy, Spain and France in the 17^th century. Geranium includes around 422 species of flowering annual, biennial and perennial plants. Both Geranium and Pelargonium are genera in the Family Geraniaceae. Linnaeus originally included all the species in one genus. Geranium, but they were later separated into two genera by Charles L’ Heritier in 1789. Although members of the Pelargonium genus are often referred to as ‘Pelargoniums’ to try to avoid confusion, many people still use the older common name of ‘Geranium’.

Most of the Pelargonium plants cultivated in Europe and North America have their origins in South Africa. Geranium robertianum is found throughout Europe, Asia and North Africa where it grows on a variety of soils, rocks, tree trunks and decaying organic matter such as logs. It is a component of virtually all forest types there. In the Pacific Northwest it is primarily found west of the Cascade crest although it extends along the Columbia River in Klickitat County. Ecological structure of Geranium robertianum L. populations under natural conditions and in the garden in Poland. Geranium farreri found in China, in south Gansu and north-west Sichuan.

Poinsettia (Euphorbia pulcherrima) is one of the most popular ornamental pot plants belonging to the family Euphorbiaceae. The botanical name Euphorbia pulcherrima means very beautiful. Euphorbia pulcherrima the name originally given by German botanist Karl Ludwig Wilenow was changed to “Poinsettia” in honour of “Mr. Poinsett” in 1836 by William Prescott. Poinsettia is a short-day, non-food and non-feed vegetatively propagated ornamental plant. Poinsettias are fascinating winter blooming small shrubs or trees which can grow anywhere from about 0.6 to 5 m tall. The coloured bracts of poinsettia plants are actually leaves. Colours of the bracts can be red, pink, orange, white or marbled. These coloured bracts are caused by photo-periodism. Poinsettias have also been called the lobster flower and the flame-leaf flower due to the red colour. California is the top U.S. Poinsettia-producing state.

ORIGIN AND DISTRIBUTION

The poinsettia (Euphorbia pulcherrima) is the world’s most economically important potted plant but despite its preeminence it is not clear which wild populations are ancestral to the varieties cultivated around the world. Tradition holds that the U.S. envoy to Mexico, J. R. Poinsett collected the progenitors of the over 300 varieties in global cultivation on an 1828 excursion to northern Guerrero State, Mexico. It is unknown whether the contemporary cultivars are descended from plants from Guerrero or whether germplasm from other parts of poinsettia’s 2000 km long distribution entered into cultivation during the nearly 200 years of subsequent poinsettia horticulture.

The combination of nuclear and plastid haplotypes characterizing cultivars was found only in northern Guerrero. Whereas, distribution modeling indicated that suitable habitat conditions for wild poinsettias are present in this area consistent with their likely wild status. Finding shows that the area of northern Guerrero as the cultivated poinsettia’s probable ancestral region congruent with the traditional account attributing the original collections to Poinsett (Trejo et al., 2012). Poinsettias are originally native to the central and southern parts of Mexico where they grow as a shrub up to 5 m. The poinsettia was introduced to the United States by Mr. Poinsett in 1828. It was introduced into North America in1838 from Mexico and since that time it has achieved considerable economic importance as a pot plant in the florist trade.

The apparent reasons for undesirable productivity under intensively cultivated areas are depletion of native nutrient reserves, emergence of multinutrient deficiencies, and consequent decline in factor productivity of applied nutrient (Dwivedi et al., 2006; Yadav, 1998; Yadav, 2003). Surveys conducted at PDFSR in different agro-climatic regions of Indo- Gangetic Plain indicated that in order to achieve previously attained yield levels, farmers have started applying greater doses of N than the recommended ones (Dwivedi et al., 2001; Sharma, 2003). Such indiscriminate use of N is likely to further worsen the nutrient imbalance in soil-plant systems, besides increasing the pest incidence, cost of production and environmental problems (Dwivedi et al., 2001). The annual productivity of rice - wheat could hardly exceed 9 t ha^-1 in the multilocation experiments on yield maximization (Dwivedi and Singh, 2011). On the other hand, long-term experiments (Singh and Mishra, 2010) and other studies indicate that crop productivity can be sustained with balanced fertilization, taking into account the emerging deficiencies of secondary and micronutrients (Tiwari et al., 2006)

Nutrient management is a major component of a soil and crop management system. It becomes even more important in intensive high yielding cropping systems because of the higher investments at stake. The SSNM is applying those concepts to areas within a field that are known to require different management options from the field average. Site specific nutrient management is a concept that can be applied to any field and any crop. Yet, it is most often thought of in relation to use of computer and satellite equipment, and does not require large farming operation. The technology tools certainly expand the capabilities for using site-specific management. This is really a “repackaging“ of management concepts that have been developed and promoted for many years.

India is a leading vegetable producing country in the world. Presently it occupies 7.981 million hectare area with the annual production 129.077million tones (2008-09). The country being belessed with the unique gift of nature of diverse climate and distinct seasons, make it possible to grow an array of vegetables number exceeding more than hundred types. However, potato being the staple food and easy to mix in several preparations ranks first (26.6%) in total production of vetetables followed by onion (10.5%) and other important Solanaceous vegetables like tomato (8.6%), brinjal (8.0%). Cauliflower and cabbage are most preferred winter vegetables and their total share in the country’s vegetable production is 5.1 and 5.3%, respectively. Other important vegetables, which are primarily grown in the country, are okra, onion, vegetable peas and a good range of cucurbits (Fig. 1)

There is miss-match in area production and productivity of vegetables among various states of the country. The West Bengal ranks first in the total production of vegetables in the country and its production during the year 2008-09 was in tune of more than 22.704 million tones, contributing the more than one-fifth of total country’s vegetable product ion. Uttar Pradesh, Bihar and Orissa in north India and Maharashtra, Tamil Nadu and Karanataka in south India are the other leading vegetable producing states in the country. In last one decade, there has been considerable progress in enchancing the productivity of vegetables which is presently 16.2 tonnes hectare^-1. Incessant growth of urbanization, ceaseless fragmentation ofland holdings and depleting natural resources are the major challenges before the expansion of any agricultural commodity either cereals or vegetables. Hence, our attention must be focused on vertical expansion strengthened with the boon ofthe technology instead of horizontal expansion just by increasing the crop area. To ensure the nutrition security of the burgeoning population of the country, it is estimated that up to 2020, the country’s vegetable demand would be around 155 million tones (Rai et al., 2004; Pandey et al., 2009). To achieve this target, it is sine qua non to integrate the various technologies right from production to post-harvest.

Protected cultivation of horticultural crops offers the best choice for diversification from traditional agriculture production system for a number of reasons. Production of crops under protected structures has great potential in augmenting production and quality of vegetables, flowers and in some fruit crops in main and off-season and maximizing water and nutrient use efficiency under varied agro-climatic conditions of the country. This becomes relevant to growers in India who have small land holding. They would be interested in a technology, which helps them to produce more crops each year from their land, particularly during off-season when prices are higher. The commonly protected structures are polyhouses, poly-tunnels, floating row covers, clotches, shade-net, insect-proof net and plastic-mulches (Chandra et al., 2000 ; Singh et al. 2010). These protected structures act as physical barrier and play a key role in integrated pest management by preventing spread of insect-pests and viruses which causes severe damage to the crop (Singh et al., 2003). This technology has great potential especially in periurban agriculture in near future, since it can be profitably used for growing high value vegetable crops like tomato, cheery-tomato, coloured peppers, parthenocarpic cucumbers, cut-flowers like rose, carnation, Berbera, lilium and fruits like strawberry, grapes etc. and for off-season cultivation of vegetables and their healthy and virus-free seedling production.

Protected cultivation is intended to mean some level of control over plant micro-climate to alleviate one or more of abiotic stresses for optimum plant growth. The microclimatic parameters being referred here are temperature, light, air-composition and the nature of rooting medium.

Rice-wheat cropping system is the most important food production system in India. This system has enabled the country to attain food security during last three decades and provided livelihood to millions of farming families in recent times. The productivity of this system has now started showing signs of decline or stagnation at many places. There are also concerns of declining soil health and other related environmental problems. At the same time, food demand is rapidly increasing due to increasing population and income growth. During the past 30 years, agricultural production has been able to keep pace with population demand for food. This came about through significant area and yield growth. Area growth was a result of new lands being farmed and through increases in cropping intensity, from a single crop to double or even triple crops per calendar year. Area growth will be less important in its contribution to production growth in the future as more land is used for urban areas and industry. Yield growth will have to be the mainstay for providing the means for meeting future food demands unless food imports start to play a major role. Total factor productivity is declining and farmers have to apply more fertilizer to obtain the same yields. There is, therefore, a huge challenge ahead to meet future food demands without damaging the natural resource base on which agriculture depends, producing food at a cost that is affordable by the poor, and with incentives to farmers that allow them to improve their livelihoods. Water will become a major limiting factor for sustained production in the next decade. Rapidly growing urban areas and industry will compete with agriculture for good quality water. There are already reports of declining water tables in some areas leading to more costly pumping of groundwater and increased costs of production.

This chapter describes various conservation agriculture technologies to attain the goal of increasing productivity and meeting food security needs while at the same time efficiently using natural resources, including water, providing environmental benefits and improving the rural livelihoods of farmers. The major success in the last few years has been the development and deployment of conservation agriculture technologies (CATs) with farmers in the rice-wheat systems. CATs range from simple surface seeding, where wheat seed is broadcast on the non-ploughed soil; no-till with a special opener for placing seed in the soil also without ploughing; reduced tillage and bed planting. This has benefited farmers through less cost, more yields and more income. CATs are defined as any practice that will result in improvement of the efficiency of natural resources. These technologies are rapidly gaining popularity among farmers as they result in higher production at less cost with significant benefits to the environment and more efficient use of natural resources. This ultimately results in higher profits, cheaper food, and improved farmer livelihoods. Crop diversification is also easier as less land is needed to produce staple cereals, freeing up land for other crops.

Cost is the value of the factors of production used in producing and distributing goods and services. The cost of a factor unit equals the maximum amount which the factor could earn in alternative employment. Broadly speaking, cost is the measure of what has to be given up in order to achieve something. Production costs play an important role in the decision of the farmers. Cost of production here means the exogenesis incurred per unit of output. In general, at given level of prices, a farmer can increase his farm income in two ways i.e. (i) by increasing production or (ii) by reducing the cost of production. The cost of production involves both cash cost and non cash cost items. Cash costs are incurred when resources are purchased and used immediately in the production process such as casual labour, fertilizer fuel etc. And non cash cost like depreciation on farm building and equipment and payment made to farmer himself or family labour, management and owned capital. Before estimating the cost of cultivation/production, we should know the concepts/terms are used in estimating the cost. Concept means idea underlying or general notion.

Cost concepts and items of cost

The cost of production of a crop is considered at three different levels viz., cost A, cost B and cost C. The concept of nine costs such as is followed by the Directorate of Economics and Statistics, Ministry of Agricultures Government of india in their cost studies are given below.

Ethnobotany deals with different aspects of man-plant relationship with special emphasis on ethnic community of a region, nay country or any geographical territory. This perhaps gives us a new dimension, orientation and objectives to make it complete with vivid information on people and their resource use pattern including science background behind scientific use. The new form of ethno-botany is now reformed and re-oriented as beta-ethnobotany. Earlier taxonomists, anthropologists and social workers have made their work on a wide field of man-plant relationship which was very loose in the sense that no specification or special focus was there which has its origin so called alpha-ethnobotany. Beta-ethnobotany deals with objective based oriented studies, divine, dynamic and quantitative ethno-botany with special emphasis to assessment of credibility of folk claims, ethno pharmacology, ethno-pharmacotherapy and ethno-medicine. Presently scientists estimated that out of over 5000 plants have been used in ethno-medicine much less than 1000 are utilized in herbal drug industry in different indigenous systems of medicine like ayurveda, homoeopathy, unani and siddha. A wide variation of use and conservation strategies on that medicine or the raw sources of these materials are available in the lower tract of Chotanagpur plateau in red lateritic part of West Bengal so called the ‘ rarh’ region. Tribal people like santal, kol, sabar, kheria, oraon, munda etc. inhabiting the rarh region use the medicine as ethno-medicine since time immemorial and conserve the plants and similar plant propagules in their home gardens and nearby sal (Shorea robusta) dominated forests for future generation. Wide application and conservation strategies of a large number of ethno-medicine and ethno-medicinal plants have been presented here with special emphasis on landscape conservation to make the ecosystem pristine rather than degraded in near future.

Several ethnic communities inhabiting in the biodiversity-rich state of Meghalaya are primarily dependent upon the forest for their livelihood needs like food, vegetables, materials for construction of houses, firewood, medicine for curing numerous ailments and many other purposes. They have acquired vast knowledge about the wealth of plant and their utilisation. The present study was aimed to document such less-known wild edible plants used by Khasi people of the state. During the current investigation, a total of 94 species of such importance belonging to 80 genera under 47 families were documented. Among these, 46 are herbs followed by 29 shrubs, 12 trees and 02 are creepers. Based on the availability and commercial potential, 37 species have been sold in local markets for income generation. Of which 17 species are sold as fruit, 11 as leaves, six as root, rhizome and tuber, five species as a stem, two as inflorescence and one species sold as fronds. All the plants are arranged alphabetically in the tabular form, followed by families, vernacular name(s), plant part(s) used, habit (s), distribution and methodology. It has been observed that the knowledge of less known wild edible plants is significantly declining day by day. These plants are known for their importance in as food nutritional supplement and its medicinal value; if the information is not collected and documented, it may not last soon.

North East India is a hub of ethnomedicinal diversity. Nearly 8,000 flowering plants are distributed and approximately 200 ethnic tribes and subtribes reside in the region. The cross-cultural diversity of the ethnic tribes and subtribes has enriched the ethnomedicinal practice in the region. Due to inaccessible terrains the ethnomedicinal practices are mostly confined to this region than the rest part of the country.More than 175 dialects are practiced in NE India. The ethnomedicinal documentation of the region in universally accepted languages was started in early 21^st century. The ethnomedicinal documentation requires multidisciplinary aspects viz., correct identification of plant species, updated nomenclature, geographical area of occurrence, IUCN status, conservational approach, vernacular names of the species, formulations, documentation of traditional knowledge informants, medicine men, specific indications of treatments, mode of preparation, mode of consumption etc, Optimum and sustainable utilization of ethomedicinal practice holds a good future for economic and societal development of the region. An insight covering various factors like challenges, scopes, legislative modalities of ethnomedicinal practices are discussed here along with a compiled list of commonly used ethnomedicinal species of the region.

Pear (Pyrus spp.) is a superior temperate fruit with good taste and flavour. Because of its tolerance to wide range of soil and climatic conditions, it is grown both in sub-tropical and temperate conditions. In J&K, 14,532 hectare area is under cultivation, with an annual production of 88,329 metric tones. In Jammu Division, pear is being grown on 7,600 hectare having 30,257 metric tonnes production annually. The pear is grown in Doda, Poonch, Rajouri, Udhampur and Kathua districts of Jammu Division.

Climate and Soil

Pear is adapted to wide range of climatic conditions. Most of the commercial cultivars require 900-1000 hours of chilling below 7^oC to break the dormancy. Pear is a temperate fruit crop; however, it can be grown successfully in subtropical regions of Jammu. There are several low chilling cultivars, which are well adapted for cultivation under sub-tropical conditions. Pears are grown successfully over a wide range of soils provided they have sufficient moisture. Compared to apples, they are less tolerant to drought and rather more tolerant to impeded drainage. Deep, well drained and fertile soil give high yield.

Table cream: It is also called coffee cream, single cream or homogenized cream. It is used in coffee and tests about 20% butter fat.

Table oil expeller: It is a type of the machine that is used for extraction of the oil from the oil seeds (Plate 230).

The oilseeds crop occupies an important position in agriculture economy in the world. Indian agriculture has made considerable progress, particularly in respect of food crops such as wheat and rice in irrigated areas; however, performance has not been so good in case of other crops particularly oilseeds, pulses, and coarse cereals. Therefore, after achieving self sufficiency in food grains the government is focusing attention on these agricultural commodities. On the oilseeds map of the world, India occupies a prominent position, both in regard to acreage and production. India is the 4th largest edible oil economy in the world and contributes about 10 percent of the world oilseeds production, 6-7% of the global production of vegetable oil, and nearly 7 percent of protein meal. This sector also has an important place in the Indian agricultural sector covering an area of about 26.5 million hectares, with total production of over 25.3 million tonnes in the triennium ending 2015-16 (GOI, 2016). This constitutes about 14.8 per cent of the gross cropped area in the country. A wide range of oilseed crops is produced in different agro-climatic regions of the country. Insects-pests are one of the limiting factors in the production of oilseed crops. Management of these pest problems by using possible control techniques could increase the quality and quantity of the products. These crops are damaged by a more number of insect pests, of which some are more serious. These pests can be effectively controlled by the integration of different techniques such as use of various safe insecticides/biopesticides, some modification in cultural practices and use of pest tolerant varieties. Integrated pest management approaches will help to increase the production and productivity of oilseed crops by reducing the pest damage without any adverse effect on the agro- system and erosion in the environment. 

Pest wise information of oilseed crop and management of major pests are described below.

1.    Introduction

2.    Materials for Boat Construction

3.    Engines and Propellers

4.    Fuel Saving

5.    Development of Computer Aided Designing (CAD)

6.    Type and Size of Boats
    
    6.1    Trawlers
    6.2    Otter trawlers
    6.3    Side trawlers
    6.4    Stern trawlers
    6.5    Freezer trawlers
    6.6    Purse seiners
    6.7    Long liners
    
7.    Fishing Equipment
    
    7.1    Echo sounders and sonars
    7.2    Global positioning system (GPS)
    
8.    Global Maritime Distress and Safety System (GMDSS)
    
    8.1    Advantages of the GMDSS over the current system
    8.2    Implementation of the GMDSS requirements
    8.3    The main types of equipment used in GMDSS
    
9.    Distress Alert Transmitters (DAT)
    
10.    Automatic Identification System (AIS)
    
11.    Handling Equipments
    
    11.1    Advances in deck machinery
    11.2    Automated long line system
    11.3    Automated squid jigging machines
    11.4    Net haulers
    11.5    Net drums
    11.6    Gantries
    11.7    Stern ramp
    
12.    Side Thruster
    
13.    Satellites and Remote Sensing
    
14.    Vessel Monitoring System (VMS)
    
References

1     Introduction

2     Bacterial Flora on Fish

3     Products of Bacterial Activity

4      Bacterial Flora of Fish Caught from Different Waters

5      Fish Spoilage and Bacterial Flora

6      Bacterial Contaminations
    
7      Bacterial Contamination of Harvested Fish
    
8      Growth of Spoilage Bacteria
    
9      Scombroid Poisoning
    
10      Microorganisms on Iced Fish
    10.1     Crustaceans
    10.2     Molluscs
    
References

1.    Introduction
    
2.    Bacteria of Public Health Significance in Fish Handling
    
3.    Sanitary and Hygienic Precautions to be Followed in Fish Handling
    3.1    Requirements for the fishing vessels
    
4.    Requirements for the Landing Sites/Auction Centres
    
5.    Requirements for the Processing Centres
    5.1     Use only potable water or clean sea water for washing fish
    5.2     Never put fish on sand or any unclean surface
    5.3     Lighting and ventilation
    5.4     Water supply
    5.5     Ice
    5.6     Utensils for fish handling
    5.7     Washing of utensils and fish contact surfaces
    5.8     Workers’ hygiene and health
    5.9     Rodent control measures
    5.10     Fly control measures
    5.11     Waste disposal
    5.12     Storage of toxic substances
    5.13     Toilet facilities
    
Acknowledgement
    
References

Introduction

Bamboos are group of woody perennial evergreen plants in the true grass family Poaceae, subfamily Bambusoideae, tribe Bambuseae. Bamboos are the fastest growing woody plants (up to 60 centimeter/day) in the world. Bamboo cultivation, especially in intensively managed and productive plantation has attracted a great interest in recent years leading to huge demand of good quality planting material. The easiest and most common method for propagation is seeds. However, in bamboo due to a long flowering cycles, seeds are not available every year and being a cross-pollinating species seedling population show much variability in characters. Species like Bambusa vulgaris and Bambusa balcooa flowers rarely and do not produce seeds making propagation absolutely dependent on vegetative means. Vegetative propagation offers a tremendous scope for mass multiplication of many commercially important species. Bamboos are vegetatively propagated through clump divisions, rhizomes, offsets, layering, marcotting, culm cutting, branch cutting, macroproliferation and through in vitro techniques. Propagation through clump divisions, rhizomes and offsets yield limited number of plants. Layering and marcotting techniques are not fit for commercial production. In vitro methods offer great scope for mass multiplication, but require high initial cost and skilled manpower and thus are not viable for decentralized forest nurseries.

1.1    General Introduction, History, Ownership of Tangible and Intellectual Property
    
1.2    Patentable Subject Matter
    
1.3    Basic Requirements of Patentability
    
1.4    Novelty, Public Domain, Obviousness and Non Obviousness
    
1.5    Inventive Step (Non-Obviousness) and Industrial Application

1. Introduction
The era of genome sequencing was exploded by the upcoming of Human Genome Project (1989), coordinated by the U.S. Department of Energy and the National Institute of Health to identify all the approximately 20,000-25,000 genes, 3 billion chemical base pairs, store this information in databases, improve the tools for data analysis, transfer related technologies to the private sector, and address the ethical, legal, and social issues (ELSI) that may arise from the project. The dawn of genome sequencing began with Fredric Sanger’s sequencing almost twenty-five years ago. The time consuming and labor intensive gel preparation and their running, as well as the cost of such experiments replaced the conventional sequencing by “shotgun” sequencing.

Abstract

DNA barcoding, an additional global standard taxonomic tool for identifying plant species helps the non-taxonomists also to identify the plant species in a more reliable way with the help of devices including nanotechnology. Researchers proposed various genes or gene portions as potential barcodes either singly or in combination, yet there is no standard/ universal barcode DNA region for plants. The more accurate plant DNA barcode region would have to be from highly variable markers in the nuclear genome preferably from multiple loci and in combination with variable DNA regions of chloroplast genome, to resolve the problems that arise due to hybridization, allopolyploidization and introgression. Nonetheless, the phylogenetically informative sites might not be the potential DNA barcode regions as the former requires synapomorphies unlike autapomorphies with high levels of informative characters in the latter DNA taxonomy, but could provide insights into genetic distances between species.

Abstract
This chapter describes how modern quantum mechanical methods can be applied in a systematic manner to understand pharmacophore and mechanistic aspects of drugs. This chapter also explains why accurate computation of drug-receptor interactions is important for in silico drug designing and testing.

WHY in silico DESIGNING?
Drug designing is the inventive art of finding a new lead compound based on knowledge of its biological target and then its slow conversion to a compound of therapeutic benefit to patient termed as “drug”. This whole process comprises of long sequels of synthesizing compounds followed by their testing and activity evaluation procedures; often a seemingly endless task until desired potency level is achieved.

Introduction 
Plant disease has been highly impacted by the development of new technology. The degree of the impact depends on how much the technology affects the component steps of the system. Many of the steps in plant disease diagnosis and management depend heavily on accurate and timely information and so can greatly benefit from the development of improved methods of accessing and disseminating information. Computers provide a wide range of tools which are extensively used in plant disease diagnosis and management. In fact, they will be a key to improve farm practices in the coming decades as it is scientifically pertinent to organize farm information in spatial databases because agricultural systems are inherently spatial. Biological and physical aspects of agricultural system create spatial heterogeneity, and as a result, patchiness is the rule in the occurrence and distribution of plant pathogens and disease (Campbell and Madden, 1990). In the area of plant diseases computers are useful

Introduction
Science and its development have had a great impact on the biological research. The main problem arises to understand and interpret the data which are the results of the higher dimensional research in the above mentioned field, and it is extremely difficult some times to give answers for complex and tedious biological questions generated through the large datasets. Parallel developments of statistical methods provide relaxation over these issues. The statistical method by bioinformatics presents many new and logical problems for researchers, scientists, and scholars related to these communities. Thus the knowledge of statistical concepts and techniques are prime important in this chain. The effort is made to cover stochastic theory, finite Markov chains in learning models to diffusion processes in population genetics, advanced statistical methods with Hidden Markov model and provide some literature on Bayesian computation like MCMC and their resources. Fortunately the applied aspect of probability theory and theory of stochastic processes seem to have been extensively used in the research and development level of biological field but the lacking of required material is a very problematic aspect in the system with proper and systematic ways.

Origin and history: Apple is a “King of temperate fruits” and it’s belonging to family roasaceae. Plants are deciduous in nature. The apple fruits are well known for its sweet, pomaceous fruits. Apples have been grown for thousands of years in Asia and Europe, and were brought to North America by European colonists. Apples have religious and mythological significance in many cultures, including Norse, Greek and European Christian traditions. Apple is the premier table fruit of the world. It is typical temperate fruit tree, about 40 percent of the world’s supply being produced in China. Major apple growing countries are U.S.A, Italy, west Garmany, France, Japan and China. In India a large number of apple variety were introduce in 1887 by Mr. Alexander Coutts in his orchard called hillock head in Mashobra near Shimla, Himachal Pradesh. It was also introduce in Bangalore and Nilgiri hills (Tamil Nnadu). But it was destroy by woolly aphid in 1897. In India apple is commercially cultivated in Himachal Pradesh, Uttar Pradesh, Uttarakhand, Jammu and Kashmir and Arunanchal Pradesh.

1.    Marketing Scenario of Fruits and Vegetables
    
2.    Marketing Systems of Fruits and Vegetables
    
3.    Vertical Coordination in Food Chain
    
4.    Supply Chain Management
    
5.    Post Harvest Management
        
References

Livestock diet or ration may be made up of one or several ingredients depending on the species of animal, their physiological state, source of feed and its nutritional composition, nutritional requirements of the animals and voluntary intake of the feed. Chemical and nutrient composition with each ingredient varies tremendously and no single feed ingredient is complete in its nutrient composition with respect to animal’s requirement.  Therefore, animal feed has to be balanced properly to supply required nutrients in appropriate quantity.  Feed formulation is an art of quantitative application of science of nutrition for the optimum supply of all the essential nutrients through actual feeding of animals. This exercise may be as simple as mixing of few ingredients with simple mathematical calculation or preparation of least cost balanced ration by computer programme.  Since the expenditure on feeding is substantial in livestock production, feed formulation needs special attention of nutritionists or feed technologists. The instruments and equipments used for feed formulation and estimation of micro minerals and ingredients like Feed grinder and mixer of capacity 75 kg, Fibertech for fiber estimation, Hammer mill-particle size reduction, Mixer and grinder for feed and fodder (complete feed preparation) of capacity 250 kg, Roughage processing-Chaff cutter, Soxhtech for fat estimation, UV-vis-Spectrophotometer and Voltameter for micro mineral estimation are shown in Fig. 3.1 to 3.8.

Analysis of Variance
    
Completely Randomised Design (C.R.D.)
    
Randomised Block Design (R. B. D.)
    
Latin Square Design