eChapters

Chi-square test is a non-parametric test as the null hypothesis does not contain any assumption regarding the population parameter. Chi-square is also written as c₂. In this chapter two uses of chi-square test will be dealt with. They are the following :

It is supposed that man was evolved on earth about 15 lakh years ago. This man started to move by standing erect on his feet and called Homo erectus L. Later the modern man was evolved called Homo sapiens L. (Sapiens means learning habit) due to his continuous learning habit. It is difficult to trace the exact period of beginning of agriculture. Archeological evidences indicated that, Agriculture started about 50,000 years ago.

Hunting was the primary source of food in olden days and was prevailed for long time. They obtained his food through domestication of some animals like dogs, horse, cow, buffalo etc. They live in the periphery of the forest and migrate from one place to another in search of food. It was not comfortable and they started enjoying by settling at one place near the river and started agricultural system.

Seed is the living link between plant and progeny and biologically seed is ripe fertilized ovule and a unit of reproduction of flowering plant. Agronomically, a seed material or propagule is the living organ of crop in rudimentary form used for propogation.

Propagation Asexual Sexual Seed Stem cutting Ex: Sugarcane Grafting Rooted slips Ex: Grasses Tubers Ex: Potato Budding Corms Rhizomes Layering

Plants perpetuate / multiply either sexually or asexually. Sexual methods are through seeds and asexually through vegetative parts. Most of the crop plants produce viable seeds which are used for sowing. Those used for multiplication are called seeds and those which used for human consumption are called grains.

Seed consisting of intact embryo, stored food and seed coat which is viable and has got a capacity to germinate. Embryo has two parts:

Pineapple is one of the most important commercial fruits of the world. It is good source of vitamin A, B and fairly rich in vitamin C, minerals like calcium phosphorus and iron. Fresh fruit of pineapple is used as dissert fruit in the form of slices. Fruit is also used for juice, squash, jam, mixed jam and in canned form, fruit cone is used for preparing candy. Some cultivars of pineapple are used for extraction of fibre. The important states of pineapple growing are Assam, West Bengal, Tripura, Kerala, Andhra Pradesh, Bihar, Karnataka and Madhya Pradesh (Bal, 1997).

Growth is the fundamental property of all living organisms. It is an irreversible permanent increase in size, volume or mass of a cell or organ or whole organism accompanied by an increase in dry weight. Where, development is sum total of growth and differentiation. It is governed by both environmental and internal factors. The development of a plant is highly complex phenomena (Table 1).

The propagation of plants has been one of the fundamental occupations of mankind since civilization begins. Most of the selected plant would have lost or could have attained undesirable forms if they were not propagated under strict environment conditions. Growth, in all nature, result from the division of cells to form more similar cells. Thus bacteria reproduce by simple division, in higher plants and animals, a new individual is genetically formed only when cell division fallows the fusion of two cells. This is known as sexual reproduction. There are thus two methods of producing new plants, sexual and asexual or vegetative reproduction.

I. Sexual Propagation

Raising of plants by means of seeds is called sexual propagation. There are many plants raised for our garden from seeds. The plants produced through seeds are called seedlings. In sexual method, the sex organs of flower are involved in process like pollination and fertilization, resulting the formation of seeds. eg rootstocks plants of many fruit crops are grown through seed propagation.

Maturity indices and their adoption at harvesting and in post harvest management of fruits provide a information against quality loss, microbial decay and deterioration of external appeal. Fruit harvesting at proper stage of maturity has direct effect on quality and market value of produce. Stage of harvesting also influence the after harvest enzymatic activities of the horticultural produce, which determines the levels of different pigments, sugars, acids and vitamins in fruits, flowers and vegetables. Maturity can be described as the attainment of a particular size, stage after which ripening takes place. However, horticultural maturity is defined as the stage of development when a plant or plant part possess the prerequisites for utilization by consumers for a particular purpose. Cucumbers, lettuce and peppers are harvested at various stages (Horticultural maturity) prior to reaching physiological maturity, or completion of growth phase.

There are many methods to determine produce maturity. The most common is the size of the individual commodity.Some other commonly used crude methods are colour change, Softening of the tissues (fig,sapota) ease of detachment from the stalk (Sapota, annona), shrivelling of fruit stalk (water melon), time elapsed from the date of flowering to picking maturity, sound by tapping jack and water melon when ripe produce hollow and dull sound on tapping but produce metallic sound if unripe etc. Another physical characteristic is the firmness and it is determined with a pressure tester. Firmness is often correlated with chemical changes that occur during ripening. Chemical characteristics include TSS, acidity, sugar, vitamins and pigments. There are certain test which accurately gives the maturity measurements such as use of colour charts (judge colour), penetrometers (firmness), brix: acid ratio etc. Other recent methods viz. thermal conductivity X-rays and NIR have also come in practice to judge the maturity of fruits and vegetables. Some commonly use maturity indices are given below in some of the important fruits.

Rapid urbanization and industrialization has led to continuous deterioration of air quality, which is a major environmental problems in many urban areas in both developed and developing countries. Air pollution is characterized by high concentration of suspended particulate matter, oxides of sulphur and nitrogen resulting primarily from increased use of vehicles. Air pollution effects are not limited to the short term nor to the plant damaged or killed. Rather, air pollution has long term effects that affect plants including fruit trees. Horticultural crops are injured when exposed to higher of abnormal levels of various air pollutants. The injury symptoms range from visible marks on foliage, reduced growth and yield to premature death of the plant. The development and severity of injury depends not only on the concentration of the particular pollutant, but also on a number of other supporting factors. These include the duration of exposure to the pollutant, the plant species and development stage as well as conducive environmental factors.

ABSTRACT

A Distributed Database is a database that is under the control of a central database management system (DBMS) in which storage devices are not all attached to a common CPU and may be dispersed over a network of computers interconnected through internet. To ensure that the Distributive databases are up to date and current, there are two processes: replication and duplication. Replication involves using specialized software that looks for changes in the distributive database. Once the changes have been identified, the replication process makes all the databases look alike. The replication process can be very complex and time consuming depending on the size and number of the distributive databases. Duplication on the other hand is not as complicated. It would basically identify one database as a master and then duplicates that database. This is to ensure that each distributed location has the same data. In the duplication process, changes to the master database only are allowed. This is to ensure that local data will not be overwritten. Both of the processes can keep the data current in all distributive locations. The most commonly used databases are DB2, MySQL, Oracle, MS-Access, Ingres, Firebird, MS SQL Server, Teradata, Sybase, Informix and Postgre SQL. Use of single or combination of databases depending on the requirement is key to success. This distributed data management with different levels of transparency like network transparency, fragmentation transparency, replication transparency, etc. Increase reliability and availability of data to the users as well as reduces the database downtime and increase the database performance, reduces network failure due to reduction of network traffic.

India has witnessed tremendous increase in the production of horticultural crops especially fruits since independence. Due to specialized scientific interventions and application of latest research tools, the country is now among the top fruit producing countries of the world ranking second next only to China. However, the productivity has still remained low as against area under the fruit cultivation. Several neglecting issues related to the production technology have remained unattended so far. The old and senile orchards are now reverting towards a declining trend of production because of plant age factor, non-compatible varieties and poor canopy management (Baba et al., 2011).

In the recent past declining productivity of old and dense orchards existing in abundance has become a matter of serious concern for the orchardists, traders as well as scientists. In India 30-35 per cent area under fruit crops is occupied by old, dense and diseased orchards. For overcoming the problem of unproductive and uneconomic orchards existing in abundance, large scale uprooting and replacement with new plantations (rehabilitation) will be a long term and expensive strategy. Therefore research efforts were initiated to standardize a technology for restoring the production potential of existing plantations by a technique called Rejuvenation. The term ‘Rejuvenation’ means renewal or making new or young again. As applied to the orchard tree it would mean restoring the productive capacity of the fruit trees. The meaning of ‘Rejuvenation’ according to Chamber’s dictionary is ‘to recover youth character or to grow again’. Obviously, this would apply to those plants which have attained a stage where they are no more profitable from the grower’s point of view.

Increasing susceptibility of vegetables to various biotic and abiotic stresses and very high cost of hybrid seeds has warranted the attention of the vegetable growers to improve the nursery raising technology of vegetables. Now-a-days protected nursery raising of vegetables has become a full flagged industry in several developed countries like Israel, Japan, Spain, Netherlands, USA etc. In countries like Israel no vegetable grower is growing his own nursery, but they are getting the required kind of seedlings from an established nursery owner on pre- order basis. Similar system of nursery raising is also prevalent in several other advance countries. In those situations where vegetables are being grown under protected conditions it becomes pre-requisite to raise the required vegetable seedlings only under protected environment to get virus free healthy seedlings.

Raising nursery from seeds provide an easy and convenient way to nourish tender and young seedlings in a well managed, small and compact area for better germination of small and costly seed. In general, vegetables crops are divided into three groups according to their relative ease for transplanting. Beet root, broccoli, brussels sprouts, cabbage, cauliflower, tomato and lettuce are efficient in water absorption and rapidly form new roots after transplanting. Vegetables crops that are moderately easy for transplanting are brinjal, onion, sweet pepper, chilli and celery which do not absorb water as efficiently as crops that are easy to transplant, but they form new roots relatively quickly. The vegetable crops which are difficult to transplant are: all cucurbits and sweet corn which require special care during nursery raising and transplanting. Seedlings not only reduce the amount of time of the crop but it also increases the uniformity of the crop and harvesting as compared to direct sowing of the crops. Transplanting of seedlings also eliminates the need for thinning and provides very good opportunities for virus free, vigorous and even off-season seedlings if grown under protected conditions in soil-less media. So it becomes necessary to know the protected structures which can be efficiently used for successful raising of vegetable seedlings, suitable types of containers and growing media, method of seeding in containers in soil-less media, growing conditions and water and fertilizer requirement of seedlings along with hardening and transplanting to the field. Protected nursery raising technology is highly suitable and can be established as a small scale industry in major vegetable growing areas of our country by progressive farmers especially in peri-urban areas. By this way the vegetable growers will get virus-free healthy and off season nursery as per their requirement and it generate extra employment in rural areas.

Climate change may manifest itself as rapid changes in climate in the short term or subtler changes over decades. Generally, climate change is associated with an increasing global temperature. Various climate model projections suggest that by the year 2100, mean global temperature may be 1.1–6.4 °C warmer than in 2010. The difficulty facing livestock is weather extremes, e.g. intense heat waves, ?oods and droughts. In addition to production losses, extreme events also result in livestock death (Gaughan and Cawsell-Smith, 2015). Animals can adapt to hot climates, however the response mechanisms that are helpful for survival may be detrimental to performance. In this article we make an attempt to project the adverse impact of climate change on livestock production. There is a two-way relationship between livestock production and environmental health.

Impact of climate change on livestocks

The potential impacts on livestock include changes in production and quality of feed crop and forage, water availability, animal growth and milk production, diseases, reproduction, and biodiversity. These impacts are primarily due to an increase in temperature and atmospheric carbon dioxide (CO₂) concentration, precipitation variation, and a combination of these factors. Temperature affects most of the critical factors for livestock production, such as water availability, animal production, reproduction and health. Forage quantity and quality are affected by a combination of increases in temperature, CO₂ and precipitation variation. Livestock diseases are mainly affected by an increase in temperature and precipitation variation.

Introduction

Climate change has been realised during 1840s when indisputable evidence of former ice ages was obtained. The climate has altered sufficiently in many parts of the world even within the last few decades which will affect the possibilities for agriculture and settlement. Reliable weather records have been kept only during the last hundred years or so, but proxy indicators of past conditions from tree rings, pollen in bog and lake sediments, ice core records of physical and chemical parameters, and ocean foraminifera in sediments provide a wealth of paleoclimatic data. The standard interval adopted by the World Meteorological Organization for climatic statistics is thirty years:1901–30, 1931–60, for example. However, for historical records and proxy indicators of climate, longer, arbitrary time intervals may be necessary to calculate average values. Tree rings and ice cores can give seasonal/annual records, while peat bog and ocean sediments may provide records with only 100- to 1,000-year time resolution. Hence, short-term changes and the true rates of change may, or may not, be identifiable.

The present climatic state is usually described in terms of an average value (arithmetic mean, or the median value in a frequency distribution), a measure of variation about the mean (the standard deviation, or interquartile range), the extreme values, and often the shape of the frequency distribution. A change in climate can occur in several different ways, for example, there may be a shift in the mean level, or a gradual trend in the mean values. The variability may be periodic, quasi-periodic or non-periodic, or alternatively it may show a progressive trend . First, it is important to determine whether such changes are real or whether they are an artefact of changes in instrumentation, observational practices, station location, or the surroundings of the instrumental site, or due to errors in the transcribed data. Even when changes are real, it may be difficult to ascribe them to unique causes because of the complexity of the climate system. Natural variability operates over a wide range of time scales, and superimposed on these natural variations in climate are the effects of human activities.

Introduction

Climate-smart small ruminant production is an approach for transforming and reorienting the small ruminant production under the new realities of climate change. The main objective of climate-smart small ruminant production is to make the production sustainable for national food security, enhancement of resilience, and reduction of greenhouse gasses emission. Climate-smart agriculture is that, which increases the sustainable productivity, enhances resilience, reduce greenhouse gasses (GHG) and boost the achievements of national food security and development goals (FAO, 2013). In India, most of the rural communities earn their livelihood on smallholder livestock production system and they are very much vulnerable to climate change. Therefore, the need of the hour is to address the impact of climate change both in terms of adaptation as well as mitigation perspective (Vemeulen et al., 2013). The increase in population, the higher income, urbanization, and change in dietary preference leading to increased demand for animal products that pressurizing for higher productivity of livestock (Delgado et al., 1999; Thronton et al., 2007).

The mutton production in our country was 399 million Kg in 2012 which will be around 537 million Kg by 2020, and 840 million Kg in 2030 and 1317 million Kg in 2050. However, the requirement for mutton would be 813 million Kg by 2020, 986 million Kg by 2030 and 1408 million Kg in 2050. Similarly, the wool production in the country is 44.7 million Kg in present time (2011-2012), which have to be increased 150, 180 and 200 million Kg in 2020, 2030 and 2050, respectively. But the harsh climatic condition, shrinkage grazing resources, increase in cultivation and industrialization and decline interest of new generation for small ruminant rearing; making a question mark to meet the future demand in the current production trend. The scarcity of resources, impact of climate change and increase demand for mutton has made the traditional coping mechanism less effective (Sidahmed, 2008). Therefore, we need climate-smart sheep and goat production option that can achieve the triple win scenario of increasing productivity, adapting and building resilience to climate change through a reduction of greenhouse gas (GHG) emission (FAO, 2013; Shikuku et al., 2016).

Introduction

In the past few decades, persistent change in climatic variables coupled with global warming lead to the genesis of climatic stress. Currently climatic stress is considered as the most serious ultimatum to livestock’s growth, development, production and reproduction in tropics and subtropics including India. Livestock’s in tropical countries are most commonly affected by the menace of climatic stress. It has been shown that, climatic stress upsets animal’s body homeostasis resulting in grievous decline in livestock’s production and productivity across the world. In general, livestock adapt to the climatic insults via behavioural, physiological, biochemical, metabolic, endocrine and molecular responses. Despite of these thermal adaptation mechanisms, livestock’s witnesses the perils of climatic stress thereby incur major loss in their production and productivity. Therefore, in this changing climatic scenario, it is imperative to rigorously understand and explore the precise mechanism of thermal adaptation to generate climate resilient species which could upsurge the socio-economic status of the farmers as well as the country.

Introduction

Animals have delicate balance of heat production and heat loss, which is maintained by thermoregulatory mechanisms in response to environmental temperature and humidity combinations. When the heat loss is overrun by heat gain, heat stress occurs. During heat stress, increase in the core body temperature due to failure of homeostatic mechanism reduces productivity of the animals below their original genetic potential in growth, milk production, milk constituents and reproduction (Ravagnolo and Misztal, 2002). Impending climate change scenario is a unanimously accepted reality. Increase in the anthropogenic greenhouse gases (CO₂, CH₄and NO₂) cause radiation leading to excess warming of the earth’s environmental system. According to IPCC, the predicted temperature rises for the end of 21^st century may be in the range of 1.8^oC to 4^oC (IPCC, 2007). The climate change has complex impact on the livestock production. Temperature or heat stress will be one of the major environmental factor to in?uence the health and productivity of livestock population. The least tropically adapted livestock may be severely affected by heat stress. The manage mental and ameliorative practices can deal with the heat stress in short term, however the long term focus of developing genetic heat stress tolerance in livestock population will be a significant and impactful strategy.

Introduction

Stress represents the reaction of body to stimuli that disturb normal physiological equilibrium or homeostasis, often with detrimental effects (Khansari et al., 1990). Domestic animals undergo various kinds of stress such as physical, nutritional, chemical, psychological and thermal stress (Marai et al., 2007; Nardone et al., 2010). Figure 1 summarises the different types of stressors and their effects on animals.

Profitable broiler production demands dietary nutrients including energy, protein, mineral, vitamins and additives as per the body requirement to meet high growth of the birds. Though energy and protein are the principal nutrients required for growth of birds but the essential role of minerals in poultry production cannot be ignored. Minerals have multifaceted role in the body. They are the components of bones and other soft tissues and play significant role in acid base balance, osmotic pressure regulation and membrane permeability. Trace minerals have major role in functioning of various enzymatic, metabolic and biochemical reaction for effective utilization of nutrients. They are constituents of many proteins involved in metabolism of nutrients, immune defense systems and hormone secretion pathways. Deficiency diseases, metabolic disorders, poor growth rate, low production, low hatchability and low feed efficiency are normally observed on deficiency or imbalance supply of minerals to livestock and poultry.

Along with other trace minerals, selenium is supplemented in broiler ration as an essential trace mineral required for normal growth and production of broiler birds. It was discovered in 1817 by J.J. Berzelius.

Introduction

Diversity connotes multiplicity of variety. In a given ecosystem, diversity refers to presence of number of different species of ?ora and fauna that have ecological niches in agreement with the environment. Scaling up, there exists diversity between different ecosystems. All life forms of ?ora and fauna on earth contains genetic material, that is the repository of an immense amount of genetic information in the form of traits, characteristics, etc. Focusing on the species level, genetic diversity refers to genetic variation that is observed in a population. Thus, genetic diversity is the total number of genetic characteristics in the genetic makeup of a species. This provides in built latitude to the nature and/or the livestock breeders (including farmers) to manoeuvre/ manipulate so as evolve an improved population of desired qualities and traits. Further, genetic diversity is essential because the presence of adequate and appropriate genes in a population will make the species tide over adversaries owing to climate change and other emerging threats like new disease outbreaks. The ‘desired’ divergent gene pool provides the inherent resilient mechanism that equip the species to cope with likely challenging situation.

Over the years, with human interventions, the genetic diversity has been interfered with to meet specific needs; mostly with respect to garnering higher production and productivity. In the process, the diversity has been compromised for achieving the unitary goal. In certain cases, geographical barriers and other bio-physical constraints have also led to loss of diversity within a population. Studies on impact of climate change especially the effect of rise in environmental temperature on milk production of dairy animals indicate that global warming will negatively impact milk production. During 2020, the annual loss in milk production of cattle and buffaloes due to thermal stress will be around 3.2 million tonnes of milk costing more than Rs 5000 Crore at current price. Similarly, there would be prevalence of many emerging diseases including those with zoonotic implications owing to change in climatic conditions. Thus, the present day society is confronted with an imminent development dilemma of ecology vs. economics more specifically genetic diversity vs. higher production. It has led to volume of debates and discussions on the livestock development agenda to be pursued. The apparently con?icting interests has also generated antipathy amongst two schools of development.

Introduction

The egg production in India is 75 billion and the broiler production is 4.2 million tonnes per annum. The growth rate of layer market is 6-7 percent per annum and broiler market is 8-10 percent per annum. The Indian poultry sector is valued at INR 1 lakh cr or USD 15.38 bn. Approx. 80 percent of egg production is contributed by commercial poultry farms, remaining comes from household/ backyard poultry. Backyard poultry plays a key role in supplementary income generation and family nutrition to the poorest of the poor. It is estimated that there are around 30 million farmers engaged in backyard poultry as per 19^th Livestock Census. Backyard poultry are being practiced for its inherent strengths. Free range birds reared by the farmers have the advantage of small body size, coloured plumage, broodiness to hatch chicks, adaptation to harsh climatic condition, lower diseases and production with least investment. The birds are scavenging in the backyard, supplemented by kitchen waste and are kept in low height houses for shelter using locally available materials. It helps to improve the nutritional status of rural poor and also improve the subsidiary income of the poor people. Such stocks make minimal use of land, labour and capital and integrate well into the backyard. Backyard poultry production system is self-sustaining because of minimal needs and can be afforded by poor people of the rural areas. The high yielding white feathered chicken varieties being reared in the intensive poultry farming are not suitable for backyard / free range backyard poultry farming due to the adverse climatic and high pathogen load prevailing in such rearing practices. Backyard poultry farming is an in-separable part of the poor tribal people and it will continue to be due to its advantages. Although backyard poultry production is not an occupation per se, it often supplements household income, primarily for poor families. Strengthening backyard poultry production may help overcome poverty and malnutrition in impoverished communities. However, the effect of climate change on these birds needs to be studied for its sustenance.

Introduction

Poultry production is one of the fastest growing sectors of livestock industry in developing countries. Environmental variation is one of the major factors that affect sustainability of poultry production systems in tropical climate (Sinha et al., 2017a). Adverse climatic stress declines the production performance of chicken which causes high economic loss to the enterprises as well as the farmers. Heat stress has an adverse effect on egg production, egg weight and shell quality of laying hen (Whitehead et al., 1998). Climatic variables like temperature, humidity, radiation and wind speed that directly affect the mechanism of thermoregulation and rates of heat exchange by all animals (NRC, 2001). Heat stress is a major factor that decreases productivity and reproductive efficiency of livestock due to lower feed intake and negative energy balance (De Rensis and Scaramuzzi, 2003). Moreover, it badly affect the production due to decline feed digestibility such as proteins, fats, starch (Bonnet et al., 1997). In addition, the acute heat stress drastically decreases the reproductive performance of hens due to alterations in acid-base balance and ion exchange mechanism (Mahmoud et al., 1996). Climate change in?uences the emergence of disease and their transmission due to increases vectors, pathogens. All the above effects impact a huge economic loss to the farmers as well as the country.

Introduction

Nanotechnology and nanoparticles are increasingly recognized for their potential applications in various aspects of human animal and animal welfare like development of various healthcare or cosmetic products, nano-electronics and techniques for environmental remediation, and many consumer products. Nanoparticles, by definition, are structures that have one dimension in the 1–100 nm range. Nanotechnology involves the application of materials at the nanoscale to new products or processes. Over the past few decades, inorganic nanoparticles, whose structures exhibit significantly novel and improved physical, chemical, and biological properties, phenomena and functionality due to their nanoscale size, have elicited much interest. Nanophasic and nanostructure materials are attracting a great deal of attention because of their potential for achieving specific processes and selectivity, especially in biological and pharmaceutical applications. Nanotechnology has made a new generation industrial revolution by developing product and formulations for medical, agriculture (nano-fertilizers, nano-herbicides, nano-pesticides, recalcitrant contaminants from water, nano-scale carriers, nanosensors), veterinary care, fisheries and aquaculture, detection of nutrient deficiencies, preservation, photocatalysis, nanobarcode, quantum dots etc. It is a rapidly growing industry currently worth billions of U.S. dollars, with many potential benefits to society. Nanotechnology has the environmental applications to protect climate change and environmental pollution as nanocatalyst, light weight nanocomposite materials, nanocoatings, improved renewables and nanosensors.

This fast growing technology is already having a significant commercial impact, which will certainly increase in the future. Because of their widespread application, the commercial nanotechnology industry is predicted to increase significantly to more $3 trillion in few years. While nanotechnologies offer many opportunities for innovation, the use of nanomaterials in food, agriculture and environment has also raised a number of safety, environmental, ethical, policy and regulatory issues.

2.1 INTRODUCTION

GIS is a tool that can be used in varied disciplines ranging from civil engineering to business. Remote sensing data is often brought into GIS to have a complete data set in many natural resource application areas. Full capability of GIS is not used widely. Many a times people use GIS only for digitising maps and to do simple query on maps. But it can also be used in modeling, simulation and forecasting, only then one may regard GIS technology is utilised to its full potential. Some GIS softwares handle simple problems for location based services while other complex softwares posses advanced modules to do all types of spatial, network and 3D analysis. Integration of softwares used in natural resources study are being interfaced with GIS to create scenarios.

Remote sensing and GIS technology is useful in the following fields:

Genetic diversity is one of the fundamental character present in any population or species. It is the total variation either phenotypic or genotypic which have occured in a population through due course of evolution. Variation in any species depends on mutation, substitution (insertion/deletion) and response to selection. However, comprehensive studies on theoretical and empirical perspectives for diversity analysis may be found by evolutionary biologists to understand the factor that influences levels of genetic diversity in natural population (Lewontin, 1974). In brief, nucleotides/ protein codes are the basic unit to quantify total amount of variation. Bioinformatic tools and techniques help us to measure inferring relationships between genes, proteins or organisms through use of these DNA/protein sequences by integration of mathematical algorithms, statistical and computer databases.

Diversity analysis is popularly known as phylogenetic analysis in bioinformatic terms. The basic principle of phylogeny is analysis/alignment of characters; whether it is morphological or molecular data. For instance, in case of morphological data of a crop species, the leaf colour/stem colour/fruit colour/grain size etc. may be compared however sequence data is problem of interest for molecular phylogeny. The evolutionary history of any crop species/ organisms inferred from phylogeny analysis mostly depicted through treelike diagrams/ dendrograms. In this chapter, phylogenetic analysis based on only nucleotide/protein sequences are discussed. For analysis of phylogenetics, default assumptions (Baxevanis and Ouellette, 2006) are:

3.1 INTRODUCTION

Life cannot be sustained without adequate nourishment. Human beings need adequate food for growth, development and to lead an active and healthy life. Animals satisfy basic food requirement mainly through natural selection. But human beings have access to a wide range of foods to choose to make up their diet. Since all foods are not of same quality from nutritional point of view, man’s ability to meet his nutritional need and maintenance of good health depend upon type and quantity of foods he is able to include in his diet to satisfy hunger. Human beings need a wide range of nutrients to perform various functions in the body and to lead a healthy life. Several reports have shown that adequate intake of fruits and vegetables forms an important part of a healthy diet and low fruit and vegetable intake constitutes a risk factor for chronic diseases such as cancer, coronary heart disease (CHD), stroke and cataract formation. The nutrients include proteins, fats, carbohydrates, vitamins and minerals. The foods containing these nutrients which we consume daily are classified as cereals, legumes, nuts and oil seeds, fruits, vegetables, milk and milk products and f lesh foods like fish, meat and poultry. Some foods provide only a single nutrient and some multiples. Scientific evidence indicates that frequent consumption of fruits and vegetables can prevent oesophageal, stomach, pancreatic, bladder and cervical cancers and that a diet high in fruits and vegetables could prevent 20% of most types of cancers. Reports indicate that increasing individual fruit and vegetable consumption by 600 g per day could reduce the global burden of stroke by 19% and decrease the risk of coronary heart diseases by 31% respectively. Realizing the food value of fruits and vegetables in human nutrition the Indian Council of Medical Research (ICMR) recommends per capita use of 120g fruits and 280g vegetables per day.

9.1 INTRODUCTION

Establishment of an orchard incurs long term investment for which it needs a very critical planning. As it is a long-term investment, plants planted in the orchard should suit to the prevailing climatic condition of that area. The land remains occupied as long as 40 to 50 years. Any mistake committed during initial year is hard to rectify later on. Proper layout of the orchard ensures optimum utilisation of land and resources for successive economic production. It becomes easy for different inter-cultural operations, harvesting and transportation of the produces. Uniform distribution of plants in the selected land in various systems of planting is done after proper layout.

9.2 LAYOUT

The proper layout of orchard aims at accommodating maximum number of trees per unit land area, maintaining adequate space for proper development of the trees and convenient orchard management practices.

19.1 DEFINITION OF ORGANIC FARMING

1.According to IFOAM (International Federation of Organic Agriculture Movement), Organic Farming definition includes all agricultural system that promote the environmentally, socially and ecologically sound production system of food and fibres. IFOAM stresses and supports the development of self- supporting system on local and regional level.

2.As per the definition of the USDA study team on organic farming “organic farming is a system which avoids or largely excludes the use of synthetic inputs (such as fertilizers, pesticides, hormones, feed additives etc) and to the maximum extent feasible rely upon crop rotations, crop residues, animal manures, off-farm organic waste, mineral grade rock additives and biological system of nutrient mobilization and plant protection”.

3.In another definition FAO suggested that “Organic agriculture is a unique production management system which promotes and enhances agro-ecosystem health, including biodiversity, biological cycles and soil biological activity, and this is accomplished by using on-farm agronomic, biological and mechanical methods in exclusion of all synthetic off-farm inputs”.

1.0 Overview

Natural resources sustain life and are critical for economic development of nations. In developing countries like India where more than half the population is rural, economic development is inextricably linked with agricultural development. In these countries, the food and livelihoods security of the poor are critically associated with access to the services provided by the natural resources - land, soil, water, biodiversity and climate resources. The natural resources are finite, and with increasing population, economic growth, urbanization, and demands for food and other agricultural commodities, these are being increasingly strained. As a result, the productive capacity of the natural resource base is shrinking and deteriorating. Sustaining the productive capacity of natural resources for future generations, while meeting the food and other demands of the growing populations, is the major development challenge of the twenty first century.

In India, during the last forty years the net sown area under agriculture has remained nearly constant at 141 million ha. In the same period, the population has more than doubled (from 541 million in 1970 to 1186 million in 2010) and per capita income grew by more than 3.5 times (from Rs 14824 in 1970 to Rs 53331 in 2010). As available land resources for agriculture are finite, increases in agricultural production to meet the growing demands of the population have to come from intensification of agriculture on the same lands. As a result, the quality of soils on these lands is gradually deteriorating both at the farm and ecosystem levels. The major threats to soil quality come from loss of organic carbon, erosion, nutrient imbalance, compaction, salinization, water-logging, decline in soil bio-diversity, urbanization, and contamination with heavy metals. More than 100 million ha of the total cultivated land of 141 million ha is stated to be under some form of soil degradation.

Similarly, utilizable fresh water resources of India are finite at about 1123 billion cubic meters (BSM), of which about 690 BCM are from surface water resources and 433 BCM are from groundwater. This supply is shared among multiple uses: human and animal consumption, agriculture, urban uses, industry and for provision of environmental services. The total water demand in 2010 is estimated at 710 BCM and is projected to increase to 1180 BCM by 2050. Several parts of India are already facing severe water shortages as a result of the growing demand for water. The quality of water resources is also impacted by agricultural intensification and development, and is increasingly a matter of concern. By the year 2050, when population is expected to stabilize, the shortages will only intensify. The country as whole can be on the verge of becoming a water scarce country, with nearly all the presently estimated utilizable water resources being put to use.

Biodiversity natural resources refer to the abundance of different species and ecosystems in nature. These resources are the primary sources of present and future productive agriculture and human well being, as they provide the basic resources for use in breeding crops and livestock, soil formation and structure, soil fertility, nutrient cycling and the provision of water services. India is among the 12 mega diversity regions of the world. It is known to have more than 18,000 species of higher plants including, 160 major and minor crop species and 325 of their wild relatives. Increasing pressure from human population growth, agricultural intensification, urbanization, pollution and degradation of land, soil and water resources are having a negative impact on the plant, animal and microbial biodiversity resources of the country and their productivity.

Abstract

Soil Resource Appraisal helps in characterizing the existing land resources, assessing their suitability for various land uses and then helping the user to prepare a rational land use plan suitable for the integrated development of the area. Application of Remote Sensing (RS) and Geographical Information System (GIS) helps not only in the characterization of the resource base, but also in the generation of various thematic maps and preparation of land use and action plans for effective implementation of various developmental plans at farm level.

Keywords : Soil Resource Appraisal, Land Use Plan, Farm level Developmental Plans

1.0 Introduction

A thorough knowledge and understanding of soils is basic to the development and management of agricultural resources. About 100 years ago, man began systematic study of soils in the field and tried to separate, classify and map their geographic distri­bution. Today we have sufficient knowledge about soils; however the characterisation, classification and mapping of soils is still tedious, time consuming and costly affair. Therefore the need for adopting new and more rapid and accurate methods of mapping soils has resulted in the use of satellite images with image interpretation techniques for soil survey and map­ping.

In India for the country as a whole, soil and other land resources information is available at 1:1 M scale, which was generated from the state level mapping done at 1:250,000 scale under the Soil Resources Mapping Project of India during the period from 1986 to 1995. This information is of general nature and useful for planning on a wide canvas and not particularly useful for farm or watershed level planning in the country. For site-specific needs and for developmental works at watershed level, we need detailed farm level database at 1:10,000 scale. Once this type of information is generated at village/watershed level, this will be useful for identifying site specific constraints, generation of area and site specific agro technologies, land-use options based on soil site suitability assessment, wasteland identification, identification of prime farm lands and farm clusters for zoning and Strategic planning, preparation of farm level development plans by line departments and proactive advice and technology transfer to farmers.

Abstract

Remote sensing is the art and science of obtaining information about an object or phenomenon without having any contact with the object concerned. Geographical information system is a tool to create, manage, retrieve and query spatial databases. Remote sensing in conjunction with Geographical Information System (GIS) has wide applications in all spheres of life and especially in agriculture. Specifically, this paper reveals some of the applications of remote sensing and GIS in the field of crop condition assessment viz., crop type classification, crop stress detection, crop yield estimation and pest and disease monitoring.

Keywords : Remote Sensing, GIS, Yield Forecasting, Crop Stress, Pest and Disease Monitoring

1.0 Introduction

Remote sensing is the art and science of obtaining information about an object or phenomenon without there being any physical contact. The information is so collected using a device called a sensor. The sensors are mounted on a platform at an advantageous position. Accordingly the remote sensing is called ground based (sensor is hand held or mounted on mast), air borne (sensor is on a balloon or aero plane) or space borne (sensor is mounted on a satellite). The basic principle in remote sensing is that each and every object has unique reflecting property like human thumb impression and when the solar energy is falling on an object a part of it gets reflected, which carries the information about the object. The reflected light is converted in to reflection percentage of incoming solar radiation. This percentage of reflection can be used to work out the vegetation indices which are surrogate measure of crop condition.

Geographical Information System (GIS) is software system which enable Collection of large volume of data with spatial relation Integration of data from different source is possible Analyze such data for our requirement Present the data in different formats (maps, table and reports)

Towards the end of the third week of embryological development, the notochord induces the overlying columnar ectodermal cells of the embryonic disc to become pseudostratified neuroepithelial cells and form a spoon shaped thickening of dorsal ectoderm called the neural plate. The cranially expanded region of the neural plate forms the primordium of the brain, while the narrower region caudal to the brain gives rise to spinal cord.

The raised lateral edges of the neural plate form the neural folds while the depressed midline region of the plate forms the neural groove. Cellular proliferation at the medial aspects of the neural folds causes these structures to gradually approach each other in the midline, meet and fuse, forming the neural tube which encloses a central neural canal.

Each optic vesicle grows outwardly until it comes into contact with the surface ectoderm. At this point, the optic vesicle induces the overlying ectoderm to thicken and to invaginate against the optic vesicle. The optic vesicle becomes concave and convert into double walled optic cup. The attachment of optic cup to the brain is called optic stalk. The space between inner and outer walls disappears as they come opposed. Since invagination of optic cup occurs at the ventral margin of the vesicle a groove is formed in ventral rim of the cup which extends along the ventral surface of the optic stalk (choroid fissure) which closes at the end of embryonic period. The groove and the notch in the optic cup are referred to collectively as the optic fissure. The optic fissure begins to close by fusion of its edges soon after the optic cup is completely formed. While it is open, the fissure allows a branch of the choroid artery (Hyaloid artery) into the cavity of the optic cup.

With the development of the optic cup the lens placode invaginates into rim of the optic vesicle and forms lens vesicle. The lens vesicle loses contact with the surface ectoderm and positioned at the opening of the optic cup and forms lens.

Teratology : is the study of abnormal development. If the malformation involves an organ or only a portion of the body, it is called an anomaly. If it is so extensive great deformity of the individual occurs when the animal is designated a monster.

etiological basis of teratology : All teratogenic agents directly or indirectly ar;ect the process of cell differentiation. These agents are divided into two broad categories i.e genetic or Intrinsic factors and environmental or extrinsic factors.

Genetic or Intrinsic factors : There are several types of genetic factors that result in abnormal development.

THEORETICAL ORIENTATION

The word “democracy” is derived from the Greek roots - ‘cracy’ meaning ‘rule of and ‘demos’ meaning ‘the people’. It is governance of the people, by the people, for the people. Rule by majority is, no doubt, an important feature of this system of governance; but the more important ingredient is rule by consultation - consultation between the people’s representatives on one hand and consultation with the officers on the other. ‘Decentralization’ means devolution of central authority among local units close to the areas served. Where authority is devolved by this process on people’s institution, it is ‘democratic decentralization’.

At the instance of the Prime Minister it was decided to give the process of the three-tier administration the strictly Indian name, “Panchayati Raj” which means a system of government. Panchayati Raj was conceived system of democratic local self-Government. Earlier, Mahatma Gandhi also advocated panchayati raj as the foundation of India’s political system, as a decentralized form of government in which each village would be responsible for its own affairs. The term for such a vision was Gram Swaraj (“village self-governance”). The institution of Panchayat represented not only the collective will, but also the collective wisdom of the entire rural community.

The Balwant Rai Mehta Committee, headed by M P Balwant Rai Mehta, was a committee appointed by the Government of India in January 1957 to examine the work of the Community Development Programme (1952) and the National Extension Service (1953) and to suggest measures for their better work. The committee recommended the establishment of the scheme of ‘democratic decentralisation’ which finally came to be known as 'Panchayati Raj' which would be a 3-tier system - 'Gram Panchayat' at the village level, Panchayat Samiti at the block level, and Zilla Parishad at the district level.

This practical assignment is dealt with the appropriate extension strategies and methodologies adopted by KVK and ATIC in disseminating proven agriculture information and technologies to farmers through delivery mechanisms.

PURPOSE

To acquaint the first-hand knowledge of the KVK- Farm Science Centre and ATIC

To study the structure, function of the centre and the role of the centre in transfer of technology.

This practical assignment is dealt with the appropriate writing of script for farm broadcast and telecast. Script writing is an art for disseminating the information to the users in an effective and interesting way. In agricultural journalism, script writing plays an important and crucial role to communicate the agricultural information to the farming community with its proper content and intent.

PURPOSE

To acquaint the students with the first-hand knowledge about the principles and technique of writing scripts for farm broadcast and telecast.

To provide the students an opportunity for developing and improving their skill in writing an effective radio/television script.

This practical assignment is focused on various methodologies and techniques in organizing group discussion and method demonstration. After completion of this assignment, student will be competent enough to organize group discussions and conduct method demonstration in the village.

PURPOSE

To acquaint students to solve the problem through discussion.

To evaluate information, ideas to solve the problem and understand the problem towards development of a programme of action relative to the problem.

This practical assignment is conceptualized and operationalized to plan for preparation and use of non-projected visual aids. In traditional and modern extension communication, the communication aids are helping the communicator to communicate the message in a better way. The examples of non-projected communication aids are poster, charts, flash cards, leaflets, folders etc.

PURPOSE

To provide precise and reliable scientific information told in simple language about a single practice or item of interest in the form of (Non-Projected Visual Aids) NPVA

To serve immediate needs of the farmer.

To catch the attention of the farmer and they can easily understand it.

This practical assignment deals with operational principles and functions of the Overhead Project which governs the appropriate handling and application of OHP in real situation and also guides students in preparing different types of Overhead Projector slides for presentation.

This practical assignment is related with listening, note taking, writing and oral presentation skill, after completion of these assignment students will improve their listening, notes taking, writing skills and will also acquires good presentation skills.

PURPOSE

 To get acquainted the students about the listening, note taking, writing skills and oral presentation skills. After completion of this assignment student will improve their listening skill.

This practical assignment is conceptualized and designed for preparation of enterprise business plan and provides students the preliminary information on developing a business plan

PURPOSE

 To equip students with the preliminary knowledge on how to develop a business plan for an enterprise.

 To acquaint the students with the important aspects of a business plan which are essential for developing and running an enterprise successfully.

Introduction

The AFLP amplified fragment polymorphism also known as selective restriction fragment amplification (SRFA) technique is used to visualize hundreds of amplified DNA restriction fragments simultaneously. The AFLP band patterns, or fingerprints, can be used for many purposes, such as monitoring the identity of an isolate or the degree of similarity among isolates. Polymorphisms in band patterns map to specific loci, allowing the individuals to be genotyped or differentiated based on the alleles they carry. produces highly complex DNA profiles by arbitrary amplification of restriction fragments ligated to double-stranded adaptors with hemi-specific primers harboring adaptor-complementary 5' termini.The technique has been widely used in the construction of genetic maps containing high densities of DNA marker loci.

The AFLP protocol amplifies restriction fragments obtained by endonuclease digestion of target DNA using “universal” AFLP primers complementary to the restriction site and adapter sequence. However, not all restriction fragments are amplified because AFLP primers also contain selective nucleotides at the 3' termini that extend into the amplified restriction fragments. These arbitrary terminal sequences result in the amplification of only a small subset of possible restriction fragments. The number of amplified fragments can therefore be “tailored” by extending the number of arbitrary nucleotides added to the primer termini. Alternatively, the use of endonuclease combinations that vary in their restriction frequency can also be used to tune the number of amplicons. Generally, the abundant restriction fragments produced from complex genomes require of AFLP primers with longer selective regions. Conversely, analysis of small genomes require of only few arbitrary nucleotides added at the primer 3' termini. The resulting AFLP fingerprints are usually a rich source of DNA polymorphisms that can be used in mapping and general fingerprinting endeavors.

Introduction

The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression levels during differentiation, morphogenesis, as well as abnormal or diseased conditions. Northern blotting involves the use of electrophoresis to separate RNA samples by size and detection with a hybridization probe complementary to part of or the entire target sequence. The term ‘northern blot’ actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane; however the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. The major difference is that RNA, rather than DNA, is analyzed in the northern blot.

Sanger Method for DNA Sequencing

One necessity first know a little about the structure of DNA:

1. A segment of DNA, which is ordinarily double stranded, has a specific orientation, as it has a 5' (read as “5 prime”) and a 3' (“3 prime”) end. This can be simply thought of as a front and tail end to the DNA segment.
2. When DNA is synthesized in the lab, the two strands are separated and new bases are added to the 3' end-thus DNA is assembled from the 5' to 3' end.
3. DNA cannot be synthesized from scratch. A short piece of DNA, called a primer, is required for the reaction to begin.
4. Primers are designed such that they are able to bind to the target DNA, the binding of which is the initiator for DNA synthesis.

NTSYSpc 2.0 software is used for various types of analysis including calculation of similarity index values and construction of dendrogram. Construction of dendrogram using gel data in the form of bands. This software accepts zero-one data.

1.1 R and R studio

R has become the world-wide language for statistics, predictive analytics, and data visualization. R is not limited to statistics; it has a wide range of tools for spatial analysis and digital image analysis and can handle any process needed for image analysis flexibly. It offers a wide range of methodologies for digital image processing, from the most basic to the most complex. As an open source project, it’s freely available for a range of platforms, including Windows, Mac OS X, and Linux. An integrated development environment (IDE) for R, R Studio, allows interactive execution of R functions with user interface. RStudio is available in two formats - RStudio Desktop and RStudio Server - both available in free and fee-based (commercial) editions.

1.1.1. Download and install R

The R software setup files can be downloaded from any of the widely distributed Comprehensive R Archive Network (CRAN). To download R, please choose your preferred CRAN mirror, a location close to you. Follow the following steps:

Sensors onboard the satellite records the intensity of emitted or reflected electromagnetic radiations in the form of digital number (DN) for each pixel. These digital numbers can be transformed to more meaningful intensity units like radiance or reflectance. This process of conversion of digital numbers to radiance or reflectance is known as radiometric calibration. Sensor specific information is required to conduct radiometric calibration.

Mosaic is the combination of two or more raster images into single image. Sometimes, single image captured by remote sensing satellite doesn’t completely covers the desired study area mostly. Therefore, users resort for mosaicking two or more images captured by satellite sensors from different paths so that the whole study area is merged into a single raster image to facilitate the image processing and analysis. The pixel size of the raster images should be same for mosaicking.

In most of the cases, there is spatial overlapping among the raster images from which we want to create mosaic. These overlapping areas are handled in multiple ways which is being discussed further using R – code example. For mosaicking raster images in R, mosaic function of the raster package is used.

Garden Rakes

1.For use with moving debris.

2.Aids in the cleaning process.

3.Proper use is with two hands.

Bulbs

Floriculturists use the term “bulbous” to include a wide array of perennial plants that grow from underground organs, including bulbs, corms, tubers and rhizomes. Any plant described as bulbous will normally have a swollen root or stem or even leaves underground (Some orchids and other plants have swollen stems – above ground). Bulbs, corms, tubers and rhizomes are perennial plants organ that are part of a plant type called bulbous plants in which part of the plant has evolved into a below-ground storage unit where food created one year is used to nourish the plant in the next. They are valuable in the garden for many reasons. Spring bulbs appear early, before most perennials have started to grow again after the winter. They can also add color to containers when other plants are not ready to face the danger of frost. In summer, bulbous plants such as Alliums and lilies can provide stately interest, while autumn crocuses and tiny hardy cyclamen can cheer up the garden toward the end of the season. The purpose of the bulbous part of the plant is to store food for the plant parts that live above ground to enable the plant to survive adverse conditions. The leaf bases may resemble scales, or they may overlap and surround the centre of the bulb. A modified stem forms the base of the bulb and plant growth occurs from this basal plate. Roots emerge from the underside of the base, new stems and leaves from the upper side. Most true bulbs have their scales closely packed together such as Hyacinthus but some types, such as the Lily (Lilium), they are loose and swollen. Most popular bulbs have a papery skin, the tunic, whose function is to protect the tissues within. Some true bulbs such as the Lily do not have a tunic and are therefore, easily damaged by rough handling. Cardio crinums dies after flowering but other bulbs are perennial. Vegetative reproduction in true bulbs is by means of offsets called bulblets.