eChapters

What is FISH ?

Fluorescent in situ hybridization is a technique in which single-stranded nucleic acids (usually DNA, but RNA may also be used) are permitted to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. Through nucleic acid hybridization, the degree of sequence identity can be determined, and specific sequences can be detected and located on a given chromosome. Fluorescent in situ hybridization (FISH) is a powerful technique for detecting RNA or DNA sequences in cells, tissues, and tumors. FISH provides a unique link among the studies of cell biology, cytogenetics, and molecular genetics.

Principle of FISH

FISH involves the preparation of short sequences of single-stranded DNA, called probes, which are complementary to the DNA sequences the researchers wish to paint and examine. These probes hybridize, or bind, to the complementary DNA and, because they are labeled with fluorescent tags, allow researchers to see the location of those sequences of DNA. Unlike most other techniques used to study chromosomes, which require that the cells be actively dividing, FISH can also be performed on non dividing cells, making it a highly versatile procedure.

In genetics, microRNAs (miRNA) are single-stranded RNA molecules of 21-23 nucleotides in length, which regulate gene expression. miRNAs are encoded by genes from whose DNA they are transcribed but miRNAs are not translated into protein (i.e. they are non-coding RNAs); instead each primary transcript (a pri-miRNA) is processed into a short stem-loop structure called a pre-miRNA and finally into a functional miRNA. Mature miRNA molecules are partially complementary to one or more messenger RNA (mRNA) molecules, and their main function is to down-regulate gene expression. They were first described in 1993 by Lee and colleagues in the Victor Ambros lab .

MicroRNAs can be encoded by independent genes, but also be processed (via the enzyme Dicer) from a variety of different RNA species, including introns, 3' UTRs of mRNAs, long noncoding RNAs, snoRNAs and transposons.

The genes encoding miRNAs are much longer than the processed mature miRNA molecule; miRNAs are first transcribed as primary transcripts or pri- miRNA with a cap and poly-A tail and processed to short, 70-nucleotide stem-loop structures known as pre-miRNA in the cell nucleus. These pre- miRNAs are then processed to mature miRNAs in the cytoplasm by interaction with the endonuclease Dicer, which also initiates the formation of the RNA-induced silencing complex (RISC). This complex is responsible for the gene silencing observed due to miRNA expression and RNA interference. The pathway in plants varies slightly due to their lack of Drosha homologs; instead, Dicer homologs alone effect several processing steps. The pathway is also different for miRNAs derived from intronic stem-loops; these are processed by Dicer but not by Drosha. Either the sense strand or antisense strand of DNA can function as templates to give rise to miRNA.

I. General information

1. All cacti are succulents
2. All succulents are not cacti
3. All the genus belonging to the family Cactaceae are native of America
4. A genus in Cactaceae family which is not native of America is Rhipsalis
5. Sedum and Sempervivum are native to temperate Europe
6. Aporocactus, Rhipsalis and Schlumbergera are native of Mexico
7. Dischidia and Hoya are native of South-East Asia

I. General information

1. Cool season turf grasses are Perennial rye grass, Creeping bent grass, Colonial bent grass, Kentucky blue grass, Canada blue grass, Poa spp, Festuca spp, Agrostis spp, Lolium spp
2. Warm season turf grasses (25-35^oC) are Cynodon sp, Zoysia sp, Stenotaphrum secundatum, Paspalum notatum, Eremochloa ophiuroides, Axonopus sp, Pennisetum clandestinum, Buchloe dactyloides
3. Broad leafed turf grasses are Arachis pintoi, Dichondra repens
4. Based on mowing height, turf grasses are classified into tall, medium & dwarf
5. Turf grasses which needs high mowing height of>3.5 cm are Tall fescue, sheep fescue, Canada blue grass, fine fescue, wheat grass, orchard grass
6. Turf grasses which requires medium mowing height of d” 3.5 to 1.0 cm are perennial rye grass, Kentucky blue grass, Buffalo grass, etc.,
7. Turf grasses which requires low mowing height of d” 1.0 cm are colonial bent grass, creeping bent grass, Annual blue grass
8. Turf grasses are classified based on their recuperative capacity into fast, medium & slow

Soil and water conservation measures

Soil conservation is using and managing the land based on the capabilities of the land itself involving application of the best management practices leading to profitable crop production without land degradation.

Water erosion control

Water erosion occurs simultaneously in two steps : detachment of soil particles by falling raindrops and transportation of detached soil particles by flowing water. Therefore, water erosion can be minimized by preventing the detachment of soil particles and their transportation.

Land capability classification

Introduction: The United States Department of Agriculture (USDA) land classification system is interpretative, using the USDA soil survey map as a basis and classifying the individual soil map units in groups that have similar management requirements. At the highest of categorization, eight soil classes are distinguished, namely, arable lands (I to IV) and non arable lands (V to VIII).

Origin of land capability classification (1933) : The Georgia Piedmont Land Capability Classification (LCC): First U.S. effort to systematically determine the best use of lands by classifying and mapping erosion rates and potential in relation to both physical characteristics and agricultural capacity.

Definition: Land capability classification is a system of grouping soils primarily on the basis of their capability to produce common cultivated crops and pasture plants without deteriorating environment over a long period of time.

Classes: Land capability classification is subdivided into capability class and capability subclass nationally. Some states also use a capability unit.

The capability classification provides three major categories of soil groupings: (1) Capability unit, (2) capability subclass, and (3) capability class.

Introduction

The development of any region is reflected in its quantum of energy consumption. With a view to keeping the pace we have to grow our energy resources at a rate that commensurate to sustainable development. There is already shortage of electricity, reflected in power cuts notwithstanding enhancement of electricity production of several folds in the country. The problem is more severe in context with rural areas in India where some 70% of population live and have agriculture as the main occupation. Although statistically few thousand villages are yet to be electrified, the availability of regular supply in far off places has been a problem and the farmers are unable to derive benefits of electricity. The fast depleting kerosene is used for lighting and diesel oil for running agricultural machinery including pumps. In addition people burn firewood, agricultural waste and cow dung cake for cooking food causing irreparable damage to the ecosystem. According to Lester Brown there will be plenty of food to eat but no fuel to cook. The burning of cow dung deprives farmers the use of potential source of organic manure. Further, due to shortage of energy resources, farmers are unable to process the agricultural products to enable them to accrue more benefits. The situation is still worse in arid region where bio mass is scarce and there is no hydro electricity. In addition, farmers are unable to generate additional income due to lack of energy resources to run appropriate device in cottage industries. In this context, solar energy utilization has a tremendous potential for providing needful energy to the farmers for domestic, agricultural and allied applications leading to a sustainable development.

Introduction

Agricultural produce are being sun dried since ages as the first step to preserve the product for future consumption. It is still practised being most inexpensive over mechanical and other advanced drying systems. The most prevailing disadvantage of open sun drying is the quality losses due to insect infestation, enzymatic reactions, microorganism growth, and mycotoxin development. On the other side, sun drying is also a highly labor intensive and time consuming process prone to theft and damage by birds. It also suffers with the lack of process control and treatment uniformity (Bansal and Garg 1987, Bansal, 1987).

To overcome these disadvantages several types of solar dryers have been developed over the years (Ekechukwu and Norton 1999). These dryer may be identified into three group namely active, passive and hybrid. As regards their structural arrangement three generic subclasses were also identified: direct-modes (the solar-energy collection unit is an integral part of the entire drying system), indirect-modes (the solar collector and the drying chamber are separate units) and mixed-modes solar dryers. Natural convection solar dryers have become more suitable for rural sector and remote areas, as they work on single energy option and do not require any other external energy source (Pangavhane et al. 2002).

4.1 INTRODUCTION

The method of propagation in plants may be sexual or asexual. In sexual method of propagation, specialized reproductive cells called gametes are formed by the process of gametogenesis. Fusion of the male and female gametes leads to the development of an embryo and eventually the seed.

4.2 GAMETOGENESIS

The production of male and female gametes is known as gametogenesis. It occurs within microspores and megaspores. It involves only mitosis. It is of two types: (1) microgametogenesis and (2) megagametogenesis.

21.1 INTRODUCTION

High population pressure has forced us to plough 46 per cent of our country area for food production. The present productivity is 12.36 tonnes/ha of fruits needs to be raised to 40.0 tonnes/ha by using N : P₂O₅: K₂O with other inputs, since recommended ratio is 0.75:0.50:1.00 only.

Since cost of the inputs like fertilizers and manures are very high, hence, efficient nutrient management is essential for sustainable fruit production and protection of the environment from different types of hazards occurring due to misuse of costly fertilizers.

The soils of India and especially of arid and semi-arid regions are impoverished to plant nutrients. Considering economy, energy and environment, it is imperative to use nutrients effectively by adopting the appropriate doses proper, placement and correct timings of application and to sustain the available nutrients in soil at the optimum level.

INTRODUCTION

Potato is a vegetatively propagated crop mainly through tubers. Seed is the costliest input, which constitutes nearly half of the production cost. Due to the high cost of seed, seed replacement rate is low, which results in build up of various tuberborne diseases.To maintain high tuber yield, multiplication is done through “Seed Plot Technique” (SPT), which involves seed production in low aphid pressure period to minimize transmission of viruses and also adopt a set of cultural practices aimed at minimizing disease spread. The production of healthy seed tubers through SPT is expensive and the low rate of tuber multiplication (normally 68 times) provides only a limited quantity of quality tubers. Further, the low aphid areas suitable for producing healthy seed in the country lie in the northern plains and high hills (> 1800 m above mean sea level). Changing climate scenario is likely to shrink the window for potato cultivation and increase pathogen/vector attacks across the country thereby seriously limiting quantity and quality of seed in the country. An alternative technology of true potato seed (TPS) or use of botanical seed has shown great potential for producing both diseasefree healthy (quality) and cheaper planting materials for commercial potato production. This chapter describes the classical TPS technology and two novel potential TPSbased technologies in potato propagation.

INTRODUCTION

Information is power in the present age of rapid technological innovations; since information technology (IT) empowers people. IT emerged as the major driver of growth in all walks of life during last quarter of 20^th century and is currently in a steady growth trajectory. It encompasses almost all human endeavors including agriculture. IT can facilitate agriculture in two broad ways; (i) as a tool for direct contribution to agricultural productivity and (ii) as an indirect tool for empowering farmers to take informed and quality decisions which will have positive impact on the way agriculture and allied activities are conducted. Precision farming extensively uses IT to make direct contribution to agricultural productivity. The indirect benefits of IT in empowering Indian farmer are significant and remains to be exploited. The emerging scenario of a deregulated agriculture under WTO has brought about a greater ‘need’ and urgency to make IT an integral part of decision making by agriculture sector. The changing environment faced by Indian farmers makes information not merely useful, but necessary to remain competitive. The farmer urgently requires timely and reliable sources of information inputs for taking decisions. At present, the farmer depends on trickling down of decision inputs from conventional sources, which are slow and unreliable. Besides, the personnel who work for the welfare of Indian farmers, such as extension workers, do not have access to latest information, which hinders their ability to serve the farming community effectively. IT will constitute the primary resource in agri-clinics, agri-business centers, kisan call centers, agri-nukkads, etc. that have been launched by Government of India and other organizations to ensure inclusive growth. Potato is an input-intensive crop and IT will have a special role in making its cultivation more productive and profitable.

INTRODUCTION

India is the second largest producer of potato in the world contributing about 12.52%of production from 11.29 % area under potato with an average yield of 22.31 t/ha. Potato is grown in almost allthe states in India under diverse agroclimatic conditions. About 90% of the total potato area is located in the subtropical plains, 6% in the hills and 4% in the plateau region of the peninsular India. Indo-Gangetic plains account for about 76% of the potato area and about 87% of the potato production in the country. Availabilityof assured qualityseed is the most important production constraint in all the potato growing regions of the country.

Seed is the basic vital input in agriculture and its timely availability in adequate quantity as per demand decides the strength growth of agricultural economy in the country. Seed has played a very important role in India’s green revolution and shallcontinue to be the importantcomponent in the days to come. There is a saying “as you sow, so shall you reap”, which traditionally relates to the quality of seed determining the production, has been the wisdom of our forefathers. The traditional farmers’ practices are to save their own seeds generally selected from healthy plants from fields which are freefrom diseases pests, harvest them separately, clean, cure and then store in containers and inspect regularly for any kind of damage; or they get quality seed from other seed producing farmers onbarter basis, indicating thereby the importance of seed in their production system.

Abstract

Traditional knowledge on plants and their uses by tribal indigenous culture is helpful in the conservation of biodiversity. During 2017-2018, ethnobotanical investigations of the plants growing in Neelum Valley in District Bandipora (a part of Kashmir Himalaya) were carried out to collect information regarding different usages of the plants species growing in the region through questionnaire and interviews. Floristically, a total of 57 species belonging to 54 genera and 38 families were investigated to be used as medicine, food, fodder, timber, fuel wood, vegetable/edible fruit, resin yield and herbal tea purposes. On the basis of the floristic analysis of the study area, the leading family recorded was Asteraceae with (11%) species co-dominated by Rosaceae (9%). In terms of habit forms, herbaceous (70%) growth form is the most dominant. The most frequently used plant parts are leaves (26%) followed by whole plant (23%) and roots (19%). The plant documented cured different categories of diseases, the most common diseases such as diarrhea, fever, digestive problems, urinary troubles, rheumatism, eye diseases, skin diseases and wounds. Based on investigated knowledge, the data are discussed under sub-head as single, double and multi-diseases curing plants. The present study can provide a baseline data for future researchers, policy makers, common public, land managers, and the other stakeholders to develop scientifically-informed strategies for conservation of natural resources and sustainable use of plant resources in hotspot regions like Himalayas and other similar biodiversity rich sites.

Abstract

Wild plants have a great role in the lives of tribal peoples living in and around the world by way of providing products for both food and medicine. Such herbal plants are the core of research, which can solve several health issues as well as nutritional supplements. Keeping on the view we have presented a summary of medicinal plants used as nutraceuticals collected from various regions of India. This communication describes 35 herbal plants used as a medicine against different diseases as well as their nutritional applicability.

Abstract

Recently there has been increasing interest in plants and plant-derived compounds as medicinal agents. In Ayurveda, a wide range of medicinal uses of Pterocarpus santalinus is described. Many important bioactive phytocompounds have been extracted and identified from the heartwood of P. santalinus. The major bioactive compounds present in the heartwood of P. santalinus are santalin A and B, savinin, calocedrin, pterolinus (K,L) and pterostilbenes. These phyto-compounds present only in small amounts and have more effect than nutrients. The heartwood of the plant is used traditionally for the treatment of inflammation, diabetes, headache, skin diseases, jaundice, and in wound-healing. A wide range of pharmacological activities and health benefits of P. santalinus have been reported, including antioxidative, antidiabetic, antimicrobial, anticancer, and anti-inflammatory properties, and protective effects on liver, gastric mucosa and nervous system. All these protective effects were attributed to bioactive compounds present in P. santalinus. Most of the secondary metabolites reported from this plant are terpenes, phenolics, flavones and sterols. The present communication gives the phytochemistry and pharmacological activities of P. santalinus on health with up-to-date information.

Abstract

Natural plant products are known to prevent several deleterious diseases. This potency of plants to act as medicinal therapeutic source is due to the presence of various bioactive compounds including alkaloids, phenols, flavonoids and glucosinolates (GSLs). Among array of these compounds, GSLs are important class of plant secondary metabolites. The mechanical or physical injury to plant leads to the activation of this “GSL-myrosinase” system resulting in the formation bioactive compounds viz., thiocyanates, nitriles, isothiocyanates, and epithionitriles. Out of these compounds, isothiocyanates (ITCs) are well known for their therapeutic properties. Allyl isothiocyanate (AITC) commonly known as “mustard oil” is one of the naturally occurring ITC which is derived from hydrolysis of sinigrin, the major GSL in cruciferous vegetables like horseradish, wasabi, brussels radish etc. Due to high bioavailability in human body, it has been widely studied for its important bioactivities. Therefore, this review highlights the significant biomodulatory effects viz., antioxidant, antimutagenic, in vitro, in vivo anticancer, antimicrobial, and insecticidal activities of AITC.

Abstract

Medicinal plants in recent years attracted much attention of researchers and become the basis of modern pharmaceuticals because of their active biologically compounds. Boerhavia diffusa commonly known as Punarnava belongs to family Nyctaginaceae is a widely distributed herb that has been naturalized in many areas of the world. It is utilized as a green leafy vegetable in many Asian and African countries besides possessing anticancer, antiestrogenic, immunomodulatory and antiamoebic activities due to the presence of valuable phytochemical compounds. Medicinal value of this plant is because of presence of specific kind of phytoconstituents like alkaloids, flavonoids, triterpenoids, lipids, steroids, lignins, proteins, boeravinone A-F, glycoproteins and carbohydrates which are present in huge quantity in this herb. In view of its therapeutic importance, it is widely used in Ayurvedic, Siddha, Homoeopathy, Unani, Modern and Tribal medicinal system. Whole plant sometime its parts are utilized for curing different disorders by tribal people in India. It is a well known ethnomedicinal plant species mostly found in subtropical and tropical regions of the world.

Abstract

About 20 percent of the world’s total population is residing in the mountains and this proportion is likely to increase in the present century as environmental degradation and pollution are driving the human population for settlement in the clean and serene environment of the mountains. Himalayan region has sizeable area in countries like India, China/Tibet, Nepal, Pakistan, Afghanistan, Bhutan, etc. Remarkable progress has been made in greenhouse research and development in many countries. However, Himalayan regions which reserve unique flora and fauna have attracted little attention so far. Changing climate, fast increasing population and shrinking land and water resources are the major challenges before us to meet the food and nutritional security of the population at global level. Improvement in the production and productivity of crops are the immediate need to boost the availability of food and improve the economic conditions of the farmers.

Greenhouse technology has tremendous potential to improve production and productivity of crops even under uncongenial soil and climatic conditions. The farmers having very small farm holdings in countries like Japan and South Korea have improved their income and living standards by adopting protected cultivation technologies. Similarly, Israel has demonstrated successful crop production under desert and uncongenial climatic conditions by improved water management practices and using greenhouse technology. However, India is yet to trap the potentials of the technology. Modern greenhouses require uninterrupted huge electric power for control of environment (heating/cooling/air circulation etc), which is considered one of the major causes for global warming. High installation and running (energy) cost, high cost of production; poor government support and lack of farmer friendly /cost effective greenhouse technology are some other challenges. Appropriate modifications suiting to our requirements, as per the need of crop, climate and farmers may lead to popularization of greenhouses in the country, especially among the small and marginal farmers. Defence Research & Development Organisation (DRDO) successfully demonstrated the innovative approach to popularize low cost zero energy based solar greenhouse technology in higher Himalayan regions of the country. Such greenhouses are specifically designed using local resources and also trap natural solar radiation/ heat and maintain it for longer period. Similarly, other approaches like using mud/ unbaked bricks, double wall approach, making hot beds, blackening of walls, using water bottles, greenhouse-cum-animal shed, etc. effective to reduce the adverse effects of the environment on crops with little inputs in hilly regions. Need of the hour is to review our approach for the greenhouse technology in farmers’ prospective and device simple and cost effective innovations for fast propagation of greenhouses in the country. This will not only improve the economic status of the farmers but also provide nutritive food to their families and enhance production and productivity of various crops in the country without deteriorating the environment.

Okra has higher average nutritive value (ANV) of 3.21 which is higher than tomato, egg plant and most of the cucurbits except bitter gourd (Grubban, 1977). Its roasted seeds are substitute for coffee. It is one of the export potential crop where it accounts to 60 per cent of fresh vegetables (excluding potato, onion and garlic) exported from India. Importing countries are Middle East, Western Europe and USA.

Origin and Distribution : It belongs to the genus Abelmoschus established by Medikus, 1787, however de Candolle (1824), treated it as section of Hibiscus.   In 1924 Hochreuntiner reinstated the genus Abelmoschus of Medikus stating that calyx, corolla and stamen fused together at the base and fall as one piece after anthesis (deciduous) where as in case of hibiscus these are persistent. Though the genus is of Asiatic origin, the origin of cultigen Abelmoschus  esculentus is variable like India (Master, 1875) Ethiopia {de Candolle (1883) and Vavilov (1957)}, Western Africa (Chevalier, 1940 and Mardorct, 1947) and Tropical Asia (Grubban, 1997) and probably its might have entered India at the end of 19th century. Cultivated okra is of old world origin. It arose as a cultigen in the Abyssinian region (Ethiopia). Wild species are indigenous to Africa.  Three wild species Abelmoschus tuberculatus, A. moschatus and A. ficulneus are native to India. Introduced to Mediterranean region to Europe and Asia. But during Columbian period it was introduced to America.  It still turnout to be polyphyletic (many places) in origin. A. tuberculatus (Indian origin) and an unknown species cross resulted into A. esculentus.

Water melon is tender to frost and most of cultivars require relatively long growing season. Plant growth and fruit development are fallowed by higher temperature and abundant sunlight. Atmospheric humidity greatly regulates fruit and foliage diseases.

Taxonomy and Origin: Ealier it was classified as Citrullus Vulgaris Schrod. But in 1963 Thieret called attention to the correct name, C. lanatus (Thumb) Mansf. Africa is centre of origin of the genus. In 1857 David Living Stone, the famous missionary explorer found both bitter and sweet melons grown together in Africa and native people used them as source of water. Cultivation is prehistoric as there are pictures made in ancient Egypt. Early cultivation occurred in Mediterranean area and as far as India also and It was brought to USA by European colonist as early as 1629.

Onion

Onion is very popular vegetable crop grown worldwide. Consumption is in the form of fresh, frozen, and dehydrated bulbs and green bunching onion. Origin of Allium cepa is still somewhat mystery. Vavilov (1951) suggested that the onion originated in the area of Pakistan. Others have suggested Pakistan, Iran & the mountain areas to the north (Jone& Mann, 1963). One fact is certain that the onion has been around in its present edible form for thousands of years. Onion recorded in tombs as early as 3200 B.C. It was mentioned as a food in the bible and in the Koran with reference thought to be around 1500 B.C. In addition to being a food considered by people, plant is having certain healing power. Some early drawing indicates that onions were used in spiritual offerings.

Stem cell research is one of the most important and promising areas of research in biological science in recent times. The stem cell technology has revolutionized modern biology. It is playing a major role for improving human as well as animal health by controlling number of incurable degenerative diseases and restoration of damage to organs via transplantation therapy. Stem cells are a specific group of cells that remain in an undifferentiated state and can be found in embryos, fetuses and adult individuals. Stem cells are primitive, undifferentiated cells present in almost every tissue that have the ability to differentiate into different types of tissue like bone, tendon, ligament, cartilage etc. Bone marrow stem cell is the ideal stem cell that is unspecialized and can undergo lineage specific differentiation into bone or blood cells under different signals or environment with new special function. Several verities of stem cells have been isolated and identified in vivo and in vitro. The two broad types of mammalian stem cells are embryonic stem cells that are found in blastocyst, and adult stem cells that are found in adult tissue, bone marrow.

A. Agronomic Considerations for Good Experimentation

For laying out of any experiment (Short / long term) at on-station and on farmers field, some of the agronomic considerations are very important for better results using experimental designs. Therefore, these are discussed here in this chapter.

1. Cropping History

The crop performance and its yield is affected by the crop grown and management followed during previous years. Therefore, for meaningful interpretation and factual reflection on yield trend due to the treatment proposed to be evaluated, the details of crop grown during previous 2 years/ seasons with details of management followed especially input applied needs to be recorded.

At country level, there is major paradiam shift from cropping systems towards farming system research in XI Plan period. The mandate of Project Directorate for Cropping Systems Research, Modipuram stands changed with focus on Farming Systems and re-named as Project Directorate for Farming Systems Research. w.e.f. 1.4.2009. The All India Co-ordinated Research Project on Cropping Systems with its 69 on-station and on-farm centres representing different agro-climatic zones of the country also stands re-named as AICRP on Integrated Farming Systems. As such, the much needed methodologies and procedures for planning, monitoring and interpretation of farming systems related data are not readily available. Therefore, some methodologies and procedures which can be adopted to execute Farming Systems Research for drawing meaningful conclusions are discussed here.

14.1 Computer

Computer is an electronic device designed to accept and store data, process them and produce meaningful results under the direction of detailed step by instructions.

In 1822, Charles Babbage, a professor of Cambridge University designed a machine called an “Analytical Engine”, which could store data and perform arithmetic operations and printing out results. He is known as “Father of Computer”.

14.1.1 Basic anatomy of computer

The word Anatomy of a computer means inner structure of the computer. The study of anatomy of computer is necessary to acquire the knowledge and functioning of its inner components. Thus, essentially a computer consists of the following three main units

Bearing

Bearing is connotation of flowering and fruiting. When a fruit tree flowers and fruits, it is said in bearing condition. Different fruits have different bearing habits, inflorescences, types of flower, mode and medium of pollination, pollen viability and all these account a lot for the bearing of fruit plants.

Bearing habit

If one observes a mango tree, he/she may find it does produce the fruits terminally on a shoot and it citrus he/she finds the fruits always produced laterally in the leaf axils. The manner or patter in which a plant produces its fruit is known as bearing habit or fruiting habit. Bearing habits are specific to the kind of plant. In determining the bearing habit of any plant three points are to be considered.

The North Eastern Hill Region is the most suitable area of India for organic agricultural farming because of its numerous advantages. This region is said to be, by and large, organic by default or the farmers of this region are de facto organic producers. Truly, the farmers of this region are using very minimal or not even minimal amount of inorganic inputs like fertilizer and pesticides. Almost in all the seven states of this region, farmers are practicing traditional agriculture for thousands of years. Recently, only pockets of the region, the use of inorganic inputs have been started but the level of use is almost negligible. This low chemical input use (inorganic input) is the biggest strength in term of the possibility of organic agriculture in this hilly region. The conversion period of organic from inorganic agriculture will be much lower and easier in this region as compared to other parts of the country. Further, the conversion process will be much easier due to the readily available huge amount of forest biomass and the residues of diverse livestock population. Moreover, experimental results show that during the transition period, the farmers face the problem of yield losses, but in NE India it was observed that yield loss was minimum, sometimes nil, because farmers of this region are using very low level of inorganic inputs. Additionally, the organic products usually fetch higher prices, nearly 20-25 per cent premium as compared to the conventional products, which, may compensate the problem of yield losses during early period of conversion and thereby increase the level of income.

North-Eastern Hill Region is suitable for growing a number of high value crops and following good agricultural practices for cultivation of these crops are likely to increase farm income substantially. Enabling the state to follow systematic organic farm practices is likely to make the farming profession more remunerative. Present socio economic study indicated that the organic farm practices might be quite favourable particularly for few crops like pineapple, turmeric, ginger and cashew, which have readymade potential.

Organic farming is the form of agriculture that relies on techniques such as crop rotation, green manure, compost and biological pest control. Organic farming uses fertilizers and pesticides but excludes or strictly limits the use of manufactured (synthetic) fertilizers, pesticides (which include herbicides, insecticides and fungicides), plant growth regulators such as hormones, livestock antibiotics, food additives, genetically modified organisms, human sewage sludge, and nano materials.

There is a need to look for new means to check this menace. Chemical fertilizers are necessary to feed the ever increasing population of the nation but this needs to be supplemented with the organic manures. But organic manures are not available in sufficient quantity. Besides, they alone cannot meet the nutritional requirement of the crops and have to be applied alongwith the chemical fertilizers. Experimental results have shown that if organic fertilizers are applied then chemical use can be brought down to half. This will lead to favourable impact on the soil ecology besides saving large amounts that are being pumped to subsidize the chemical fertilizers.

Organic agriculture is picking up in some parts of our nation and it can generate viable farming option to small and marginal farmers. But short supply of the organic inputs is emerging as one of the major bottlenecks. Vermicomposting of city wastes and farm wastes can generate substantial volume of the organic fertilizer for use in our fields, thereby, helping us to overcome major hurdle in taking up organic agriculture production. This chapter attempts to identify the find out the ways and benefits of practicing organic farming and determines the economics of Vermicomposting technology.

Originating in the New World tropics, peppers have become ubiquitous culinary essential now grown not only in warm climates but in temperate areas as well. They include both sweet and hot chilli varieties. Botanically chilli belongs to the genus Capsicum. The North East region has its own unique combination of living species, habitats and ecosystems, which together make up its diversity rich resource. While speaking strictly about plant diversity, two regions of the country are termed as hot spots. These are – Western Ghats and the North Eastern hill regions. The King’s chilli is an inter-specific hybrid cultivated in the Nagaland and Assam region of northeastern India and parts of neighboring Bangladesh. In 2007, Guinness World Records certified the Bhut Jolokia as the world’s hottest chili pepper, 400 times hotter than Tabasco sauce. From time immemorial, traditionally the Bhut jolokia is been cultivated and consumed by the local people in North eastern states like Assam, Nagaland, Manipur and Mizoram. A good amount of genetic variability exists in the landraces of the crop in respect of Pod size, shape, and colour etc.

In recent years, the King’s chilli (Bhut jolokia) is gaining importance in the scientific community as it was reported as the hottest chilli. Though Bhoot jolokia or Naga chilli was cultivated from time immemorial, but nobody really bothered to compare its heat with other record setters. The field of King’s chilli is still in a growing stage and it components and their beneficial uses are still to be exploited fully. Very little research towards the scientific cultivation of this potential crop was accomplished in recent years and until now no standard scientific package of practice is available for the cultivation of the crop.

There is tremendous scope for utilizing the available genetic variability of the crop in a well-planned plant breeding programme for further development of elite genotypes in this crop. Development of proper and efficient post harvest mechanism and also of different value added products of King’s chilli shall generate a great potential for earning revenue in the national as well as international markets.

Fast increasing population, changing climate, shrinking land and water resources are the major challenges before us to meet the food and nutritional security of the population at global level. Improvement in the production and productivity of crops are the immediate need to boost the availability of food and improvement economic conditions of the farmers. Greenhouse technology has shown tremendous potential in improvement in production and productivity of crops even under uncongenial soil and climatic conditions. The farmers having very small farm holdings in countries like Japan, Holland and South Korea have improved their income and living standards by adopting protected cultivation technologies. However, India is yet to trap the potentials of the technology. Modern green houses require uninterrupted huge electric power for control of environment (heating/cooling/air circulation etc), which is considered one of the major cause for global warming. High installation and running (energy) cost, high cost of production; poor government support and lack of farmers’ friendly/cost effective greenhouse technology are some other challenges. Low cost zero energy based solar greenhouse technology popularizes the green houses in small and marginal farmers of remote areas. Such greenhouses are specifically design using local resources and also to trap the natural solar radiation/ heat and maintain it for longer period. The designs do not require electric power which ultimately reduces the installation/running cost and also does not have the adverse effects on the environment. This will not only improve the economic status of the farmers but also provide nutritive food to their families and also enhance production and productivity of various crops in the country without deteriorating the environment.

Introduction

Cucurbits, belonging to the family Cucurbitaceae are among the most ancient cultivated plants. The term cucurbit was coined by Liberty Hyde Bailey for cultivated species of Cucurbitaceae. Other vernaculars applied to the family and several of its members are ‘gourd’, ‘melon’, ‘cucumber’, ‘squash’ and ‘pumpkin’. The Cucurbitaceae consists of two well defined sub families, eight tribes representing varying degrees of circumscriptive cohesiveness and about 118 genera and 825 species (Jeffrey, 1990). It is mostly tropical and sub tropical in distribution and only a few species occur in temperate regions. Some are semi desert or saprophytic in nature. The Indian sub continent is considered to be the centre of origin for a number of wild and cultivated crops. Chakravorty (1982) reported that 36 genera and 100 species of cucurbits are found in India. Cucurbits are cultivated in 8.5 m ha area in world giving a production of 17.9 mt. While in India a total of 4.5 mt cucurbits are produced from an area of 0.42 m ha area (FAO, 2004).

Cucurbitaceae is one of the most genetically diverse groups of plants in the plant kingdom. As a family and as individual crop, cucurbits epitomize adaptive differentiation and evolutionary divergence. Not only may cultivars within a crop vary significantly in their characteristics, but the same cultivar grown in distinct areas can have different needs in response to disparate local growing conditions. Cultures and ethnic groups may have different cultivar preference and horticultural practices, which also increase morphological diversity within a crop.

Cucurbits are of tremendous economic importance as food plants. They are extensively grown in mixed cropping of more than one kind in long and meandering river beds. Of various improvements approaches possible, breeding of superior pure lines and hybrid cultivars of cucurbits utilizing locally adopted land races are the most important methods which will have far reaching impact on increasing the production and productivity of cucurbits.

Introduction

Global food productivity will have to rise significantly to satisfy an expanding world population. Increased vegetable production is one option towards the efficient conversion of natural resources into food stuffs. As a group, vegetables are a diverse range of herbaceous plants that do not belong to any particular botanical species, genus or family. Individual members may be at various stages of domestication. Vegetables are typically annual herbaceous species, whose plant parts (leaves, stems, flowers, fruits, roots or tubers) are consumed as fresh or as cooked product, and as an additional source of vitamins, minerals, fibre, phytochemicals, protein and/or calories. Health benefits are also claimed for some vegetable species, mainly due to antioxidant activity and capacity to scavenge free radicals.

Domesticated plants have been fundamentally altered from their wild relatives; these species have been moved into and adapted to new environments. They have become dependent on the farmer’s hand and they have been reshaped to meet human needs and wants. Modern crops are the results of thousands of years of these evolutionary processes. Like all biological evolution, crop evolution involves two fundamental process; the creation of diversity and selection (Harris and Hillman, 1989). Crop evolution is distinguished by two types of selection; one natural and another artificial or conscious. These evolutionary processes must continue in order for agriculture, a living and evolving system to remain viable.

In the hands of plant breeder’s sexual hybridization is a powerful tool for producing superior plants by combining characters distributed in different members of a species or different species of a genus. Genetic variability within the species has been efficiently utilized by breeders in their efforts to improve crops. However, the existing variability in a breeding population may not be sufficient for modern plant breeding purposes, and thus great efforts have been made to broaden the existing gene pool of crops through utilization of wild germplasm.

Introduction

Radish (Raphanus sativus L., 2n=2x=18) is a root cum leafy vegetable suitable for sub tropical and temperate climate. Radish probably originated in Europe and Asia. It has been under extensive cultivation in Egypt since long. It was introduced to England and France in the beginning of 16^th century. In 1806, it was introduced to America. Radish does not exist in wild stack. It is believed to have originated from R. raphanistrum which is widely distributed as weed in Europe. Radish is primarily a winter crop but with the development and release of new varieties, it can be grown all the year round. The new varieties can withstand heat and does not bolt in spring. Radish is a favourite crop because of its quick growth and availability in 4-7 weeks.

Its fleshy roots are modified roots, developed from both the primary root and hypocotyl. The leaves and roots are consumed both as salad and cooked. The radish root is a good appetizer; its different preparations are useful in curing liver and gall bladder problems. Roots are also used in treating urinary complaints and piles. The juice of fresh leaves is useful as diuretic and laxative. Pungency in radish is due to volatile isothiocyanates while pink colour is due to pigment anthocyanin.

Radish is a good source of vitamin C containing 15-40 mg per 100g of edible portion. Roots also contain proteins, fat, minerals, fibre and carbohydrates, besides trace elements like aluminium, barium, lithium, manganese, silicon, titanium, fluorine and iodine. Pink skinned radish is generally richer in vitamin C than the white skinned type. Radish contains glucose as the major sugar and also contains fructose and sucrose in smaller quantities. Pectin and pentosans are also reported to be present. The leaves are good source of extraction of protein on a commercial scale and seeds are potential source for non-drying fatty oil suitable for soap making, illuminating and for the edible purposes.

Introduction

In 1990, the magnitude of fungal diversity, that is, the actual number of species worldwide, was estimated conservatively to be at least 1.5 million. Of the 1.5 million estimated fungi, it has been estimated that 14,000 species produce fruiting bodies of sufficient size and suitable structure to be considered macro-fungi which can be called mushrooms. Of these, about 50 % or 7,000 species are considered to possess varying degrees of edibility and more than 3,000 species from 31 genera are regarded as prime edible mushrooms. To date, only 200 of them are experimentally grown, 100 economically cultivated, approximately 60 commercially cultivated and about 10 have reached an industrial scale of production in many countries (Hawksworth, 2001). Of the 14,000 species of mushrooms in the world, around 700 have known for medicinal properties. Thus, mushrooms have vast prospects as sources of medicines. The early herbalists were more interested in the medicinal properties of mushrooms than in their basic value as a source of food. Humankind has constantly searched for new substances that can improve biological functions and thereby make people fitter and healthier. About 3.5 billion people worldwide, all over half of the world populations rely on plant-based medicines and dietary supplements for their primary health care. Of the plant materials involved in medicines or health tonics, quite a few are fungi. The practice of using fungi as herbal medicines can be traced in different early records of the ‘materia medica’. The earliest book on medicinal materials the “Shen Nongs Herbal” recorded the medicinal effects of several fungi such as Ganoderma lucidum, Poria cocos, Tremella fuciformis, Polyporus umbillatus and other unidentified fungi. The most famous of all work on the traditional medicines “Pen Ts’ao Kang Mu” which was compiled by Li Shi-Zhen of the Ming Dynasty recorded the medicinal fungi totaling more than twenty species, including Ganoderma lucidum, Poria cocos, Polyporus umbellatus, Lentinula edodes, Termitomyces albuminosus, Auricularia auricula, Pleurotus ostreatus, Armillaria mellea etc. A very unique insect-infecting fungus, Cordyceps sinensis, was for the first time taken as a medicinal fungus in the book “Essentials of Materia Medica”. There have been new discoveries and developments in the field of medicinal fungi, which have greatly enriched the treasure house of our traditional medical and medicinal sciences. As a result of large number of scientific studies on medicinal mushrooms in the past three decades, the traditional uses of many mushrooms have been confirmed and new wider uses found. Some traditionally important and leading medicinal fungi in the Oriental medicines are presented below

1. INFLORESCENCE

An inflorescence is a group or cluster of flowers arranged on a stem that is composed of a main branch or a complicated arrangement of branches.

1.1. Types of inflorescence

Inflorescences can be simple or compound.

1.1.1. Simple inflorescence

Inflorescence of sessile disc florets forming the capitulum

1.1.1.1. Indeterminate

Indeterminate simple inflorescences are generally called racemose. The main kind of racemose inflorescence is the raceme. The other kind of racemose inflorescences can all be derived from this one by dilation, compression, swelling or reduction of the different axes. Some passage forms between the obvious ones are commonly admitted.

1. SEED

A seed is defined as a ‘mature ovule’ or a reproductive unit formed from fertilized ovule, consisting of an embryo, reserve food and a protective cover.

Seed is the basic input in agriculture upon which other inputs are applied. A good vigorous seed utilities all the resources and realized as a reasonable output to the grower. It is wealth to the farmer, it is the yesterday’s harvest and tomorrow’s hope. Good seed in good soil realize good yield.

Seeds exhibit a great range of variation in shape, size, colour and behaviour. The most essential factor for the success of plantation is the ready availability of quality seeds. The quality of seed is totally responsible for the future return / performance of each and every seedling.

1. TROPICAL FRUITS

1.1. Common name, botanical name and family

1. INTRODUCTION

For maintenance of genetic purity in the field with the seed crop and to test verify the standards in certification programme, the studies on varietal characterization and identification have attracted the attention of breeders, breeder’s right protection institutions, farmers, the seed industry, certification agencies, and seed testing laboratories with a view to assure the quality of seed marketed to the consumers.

Introduction

Food processing comprise of techniques employed to transform raw ingredients into final food product or to preserve the food product. Food processing industry as well as at domestic scale the food processing methods aim at providing processed food products for daily consumption by humans and animals. Food processing aims at increasing the shelf life, preserve, make nutritious or ready to eat foods, and getting the best quality final products. There are various techniques and methods which come under food processing such as drying, dehydration, fermentation, pickling, freezing, mixing, pumping, pasteurization, cooking and homogenization etc.

Rheological properties of foods are greatly influenced by various unit operations and processing methods or conditions such as heating and mechanical treatments. Processing of different food products such as dairy, fruit and vegetable products like jams and purees, chocolates, dough development etc. and basic make up of foods such as fats, proteins and others such as emulsifiers, hydrocolloids etc. affects the rheological properties of food product and which ultimately affects the quality of end product. Apart from processing conditions the compositional variations have a great impact on the textural or rheological properties of foods. The changes in rheological characteristics of a food product reflects the alterations in the molecular structure of the food material, this can aid in revealing the changes in product structure during different processing methods or conditions. In designing of various processes such as cooling, freezing and equipment design information about thermal properties is crucial. Moreover, in order to design storage structures for foods and refrigeration equipment the knowledge of thermal properties is important (Becker et al. 2003).

For rheological measurements various tests and techniques are available, such as steady and dynamic shear measurements. From the steady shear properties flow properties of the sample can be revealed whether the flow is Newtonian or Non Newtonian. In case of dynamic shear rheological properties information on viscoelastic characteristics can be withdrawn in terms of storage and loss modulus and depending on the predominance of each modulus the nature of sample can be predicted i.e. whether viscoelastic solid or viscoelastic fluid (Bhardwaj et al. 2019). Apart from these measurements, other rheological measurements are available for specific food materials, such as farinograph, alveo-consistograph, mixograph etc. are meant for study of rheological properties of dough. So, accordingly, depending upon the food material choice of rheological measurement can be made. To study the thermal transitions DSC (Differential scanning calorimeter) and DTA (Differential thermal analysis) are commonly employed. Dynamic measurements as a function of temperature or time are made in these techniques to detect the endothermal and exothermal changes. These techniques can be used to determine the phase transitions in various foods, for instance, crystallization and melting of water, lipids and other food components; denaturation of protein; gelatinization of starch and others (Roos 2015).

Introduction

Lipids are among the major components of food of plant and animal origin (food lipid). There is no precise definition available for the term lipid; however, it always includes a broad category of compounds that have some common properties and compositional similarities. Lipids are materials that are sparingly soluble or insoluble in water, but soluble in selected organic solvents like benzene, chloroform, ether, hexane, and methanol. Together with carbohydrates and proteins, lipids constitute the principal structural components of tissues (Erwin Wsowicz et al., 2004). Lipid oxidation is one of the major reasons that foods deteriorate and is caused by the reaction of fats and oils with molecular oxygen resulting in off-flavours that are generally called rancidity. Exposure to light, pro-oxidants and elevated temperature will accelerate the reaction (Velasco, J et al., 2010). Rancidity is related to characteristic off-flavour and odour of the oil. There are two major causes of rancidity. One occurs when oil reacts with oxygen and is called oxidative rancidity (Ribeiro et. al., 1993). The other cause of rancidity is by a combination of enzymes and moisture. Enzymes like lipases liberate fatty acids from the triglyceride to make di and monoglycerides and free fatty acids and such liberation of free fatty acids is named hydrolysis. Hydrolysis is additionally caused by chemical process that’s prompted by factors like heat or presence of water. Rancidity caused by hydrolysis is called hydrolytic rancidity. Oxidation cares mainly with the unsaturated fatty acids. Oxidative rancidity is off interest because it results in the event of unfavourable off-flavours which will be detected early within the development of rancidity, more so than in the case of hydrolytic rancidity. Oxidation can alter the flavour and nutritional quality of foods and produce toxic compounds, all of which may make the foods less acceptable or unacceptable to consumers. Oxidation products typically include low relative molecular mass compounds that are volatile also as undesirable off-flavour compounds (Food lipids).

5.1. Importance of Secondary Nutrients in Agriculture

Secondary nutrients such as sulphur (S), calcium (Ca) and magnesium (Mg) are the macronutrients that are required in relatively large amounts for crop growth. Among the secondary nutrients, Ca is the 5^th most abundant element in the earth’s crust while, Mg and S are ranked 8^th and 13^th, respectively. The average S content of the earth’s crust ranges from 0.06 to 0.10%; whereas, Ca in soil solution of temperate region soils is 30 to 300 ppm and soils of tropical areas is 8 to 45 ppm (Tisdale et al., 1997). In case of Mg, its’ concentration ranges from 5-50 in temperate region and 120-2400 ppm in tropical region. The Ca and Mg are the most abundant cations occupying the exchange sites of the soil colloids. Thus, most soils with the possible exception of highly weathered, leached acid soils, contain enough Ca and Mg for crop growth. In soils, occurrence and reactions of S are different from those of Ca and Mg. The plant available S is SO₄^2- ion which is relatively mobile in soil solution. Like nitrogen (N), S is also subjected to chemical and biological oxidation-reduction reactions. On the other hand, Ca and Mg are much more stable and they exist in soil as Ca²⁺ and Mg²⁺ cations and present in association with soil colloidal fraction similar to that of K. In general, Ca and Mg are the most abundant cations occupying in the exchange site.

Secondary nutrients in soils may be lost due to several reasons such as (i) crop removal, (ii) leaching, (iii) erosion, and (iv) volatilization. The amount of Ca and Mg attain importance in areas where soils are either strongly alkaline or acidic. These soils are often deficient in Ca and Mg. The Ca deficiency is most widespread in acidic soils. The alluvial soils of the Indo-Gangetic Plains (IGP), being neutral to moderately alkaline in reaction, have sufficient bases on their exchange complex and Ca deficiency is, therefore, rarely found in these soils. However, alkali soils may contain inadequate quantities of Ca due to dominance of Na⁺ on the exchange complex. Magnesium deficiency is found in sandy soils with low exchangeable Mg²⁺, acid soils and those with high in native or applied K. Alkali soils have high exchangeable Na⁺. The Ca²⁺ is a suitable ion to replace this Na⁺ from the exchange complex when Ca²⁺ is supplied through gypsum (CaSO₄.2H₂O). The replaced Na⁺ forms Na₂SO₄ which is then leached down to remove the salt away from the root zone of the crops. Gypsum also reacts with Na₂CO₃ to form CaCO₃ and Na₂SO₄ which is leached down the profile. A thorough leaching of the soil with irrigation water should be done after application of gypsum to free it from Na₂SO₄.

5.1 Introduction

Hydroponics is a method of growing plants in a nutrient solution rather than in soil. Terrestrial plants may be grown with only their roots exposed to the nutritious liquid.

Traditionally, the soil supports the crop’s roots by helping them remain upright and ensuring the delivery of water and essential nutrients. In hydroponics farming, crops are supported by an inert medium such as perlite, gravel, or other substrates. Therefore, the nutrients are supplied by using various practices that bring mineral nutrient solutions to the crops. In the present situation there excessive use of chemical fertilizers, affecting fertility of soil is badly affected. The fertility of the soil is falling that affects the plants growth but in hydroponics, the plant remains unaffected. Plants grow faster than in soil. Produce better quality of plants and higher yields. It use less water. It is possible to use hydroponics on outdoor crops, but most of the hydroponics production is in greenhouse. Hydroponic farming system has the potential to reduce the problem associated with traditional crop production. Therefore, it could serve to be a turning point in feeding the global population. The greenhouse and its environment control system are the same whether plants are grown conventionally or with hydroponics. The difference comes from the support system and the method of supplying water and nutrients.

The hydroponic farming has been successfully demonstrated by the commercial growers for fast-growing crops such as tomatoes, cucumbers, spinaches, lettuce, radishes, strawberries, bens, spring onions, coriander, and ornamentals. Indeed, hydroponics technology enables a more precise control of micro climatic conditions. In this technique, the vigorous development of a root system is taking place, which enable the uptake of essential nutrients from nutrient solution for better crop yield. The initial investment in hydroponics farming is high and required constant energy input. Owing to this, hydroponics farming is recommended for growing high value crops in developed countries such as the United States, Canada, Europe and Japan.

Abstract

Nitrogen (N₂) is an essential nutrient for every organisms. It is the building block of many biological molecules. Atmosphere contains 79% of N₂ gas which cannot be used by plants directly as it is chemically inert. Many diazotrophs like bacteria and cyanobacteria have the capability to convert atmospheric dinitrogen into a usable form by means of Biological nitrogen fixation (BNF). N₂ fixation is generally three types i.e. Asymbiotic, Associative and Symbiotic. Asymbiotic N₂-fixation also known as free living N₂-fixation is under taken by some bacteria and cyanobacteria. In associative N₂-fixation, some bacteria fix nitrogen in association with root of some grasses and cereal plants. In symbiotic nitrogen fixation, Rhizobium form root nodule in legumes and fix atmospheric N₂ and convert it into the ammonia (NH₃) by the help of Nitrogenase enzyme. This oxygen sensitive enzyme consist of two components i.e. di-nitrogenase (MoFe protein) and di-nitrogenase reductase (Fe protein) which are highly conserve in sequence and structure. The N₂ fixing genes e.g. nod gene (nodulation gene) and the nif gene (nitrogen-fixing gene) are located in the plasmid which is also called mega plasmid. Symbiotic association between legume and rhizobium have agronomic importantance and reduce the use of N-fertilizers or even avoided. The long term use of the nitrogenous biofertilizer in the agricultural field is a promising approach to develop and fulfil N₂ demand of the growing crop without causing any environmental hazard.

Abstract

Rhizobia are soil bacteria that fix atmospheric dinitrogen after establishing symbiotic relationship inside the root nodules of legumes (Fabaceae). Plant Growth Promoting Rhizobacteria are the group of bacteria which thrive in the rhizosphere region and enhance the plant growth through direct & indirect mechanisms. Pulses are being inoculated with specific rhizobial strains to have better nodulation in India. The native rhizobial strains from the root nodules of legumes from different agroclimatic zones of Odisha was isolated, characterised and screened for PGP activities. Out of all eleven isolates showed similar characteristics with the family Rhizobiacae. Among them six isolates contained nif-gene and PGPR activity (B2, B4, B5, B6, B7 and B8) were applied to seeds, that helped in faster germination and better elongation of root and shoot. The observations were statistically significant.

Abstract

Cow and buffalo milk is the major source milk worldwide contributing 81 and 15% of total milk production respectively. However, milk from animals like goat, sheep, camel, donkey, yak etc also contribute to human nutrition and health in different parts of world. These milks are source of staple traditional product and considered as delicacy in parts of Europe, UK, USA etc. The microbiota of raw milk and fermented milks, cheeses, made from raw milk is complex. These microorganisms have immense role in determining quality and safety of the subsequent product made from these milks as they may be responsible in spoilage and reducing shelf life, causing human health issues, also contribute to development of flavor and aroma formation, texture development, ripening cheese. Different factors like host, stage of lactation etc. affect the composition of microbiota present in the milks and methods like cultural and culture independent methods metagenomic analysis have been done to determine the microbial community present in the milk.

Introduction

Non- cow and non-buffalo milks such as milks from goat, sheep, camel, donkey, yaks etc. are one of the important sources of nutrition in different parts of the world. In Mongolia, the highlands of the Afghanistan and Pakistan, Himalayas in India, Nepal, and Bhutan, Tibetan Plateau and in the Mountains China yak milk is the only source of milk. Cheese made from sheep and goat milk are categorized as gourmet food and many times regarded as protected designation of origin (PDO) status. Camel milk is used for medicinal food and donkey milk is suitable for children, aged people due to its hypo allergenic properties. These milks and milk products like cheese, naturally fermented milks and yoghurt are staple traditional foods in different parts of the world.