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Definition

Seed treatment is the process by which seeds are treated with physical, chemical or biological agents to prevent the infection of seedlings and the subsequent development of the crops.

Advantages of Seed Treatments

Increased seed germination.

Ensures uniform seedling emergence.

Protect seeds or seedling from early season diseases and insect pests improving crop emergence and its growth.

Lower seedling mortality.

Improved plant population and thus higher productivity.

Reduces the cost and the potential environmental impact.

Also reduces the probability of chemical residues in harvested grain.

Relatively easy and cheap to apply compared to broadcast sprays.

Proper selection of a fungicide and its application at the correct dose and the proper time are highly essential for the management of plant diseases. The basic requirement of an application method is that it delivers the fungicide to the site where the active compound will prevent the fungus damaging the plant. The fungicidal application varies according to the nature of the host part diseased and nature of survival and spread of the pathogen. The method which are commonly adopted in the application of the fungicides are discussed.

1.Soil treatment

2.Seed treatment

3.Foliar or vegetative application

Insect Control and Insecticides

Control Tips

Practicing Integrated Pest Management can significantly reduce the amount of insecticides needed to control many insect problems.

Broad-spectrum insecticides are effective against all insects, even the good ones. Other insecticides target certain insects. Using a targeted insecticide minimizes the risk to beneficial or non-target insects.

Some insecticides work immediately to kill insects while others may need some time to take effect.

Using more than one insecticide product in the same location can increase or decrease each one’s effectiveness. It may also result in a greater risk to health and/or the environment.

Insect growth regulators like pyriproxyfen and methoprene do not kill insects; they make it impossible for exposed insects to molt (grow) or lay eggs properly.

Insecticidal baits can be used instead of spraying large areas, especially for social insects like ants. This can decrease the risk of exposure, but remember to place baits where children and pets won’t have access.

A. Purchase

a)Do not purchase leaking containers, loose, unsealed or torn bags.

b)Do not purchase pesticides without proper/approved labels.

c)Purchase only just required quantity e.g. 100, 250, 500 or 1000 g/ml for single application in specified area.

Effective application of pesticides depends on many factors. One of the more important is to correctly calculate the amount of material needed. Unless you have the correct amount of pesticide in your tank mix, even a correctly calibrated sprayer can apply the wrong rate.

Manufacturers provide application rate instructions on every pesticide label. Due to the variety of ways in which these recommendations are stated (such as lbs. of active ingredient (a.i.) per acre, lbs. of formulation per 100 gal. of spray, or ozs, of a.i. per 1,000 sq. ft.) it is often necessary to adapt the recommendations to different areas and volumes, or even other units. Sometimes the amount of active ingredient must be converted to the amount of actual product. This process can be very confusing.

Solid formulations such as dust, wettable powder or granules, the active ingredient is mixed with inert material. The concentration is expressed as:

Active ingredient (%) in the total weight of commercial product.

Calibration is the process of measuring and adjusting the amount of pesticide your equipment applies or delivers to a specific area. The purpose of calibration is to ensure your equipment is applying the correct amount of material uniformly over a given area.

Equipment is manufactured to be adjustable. Charts or tables may be provided to assist the operator in making adjustments to the settings. These recommended settings, however, are only approximate and may not be appropriate for all situations. Therefore, your equipment must be calibrated periodically. This depends on the type of equipment and the frequency of use. The application rate of the sprayer is affected by travel speed, nozzle size, and sprayer pressure. Equipment is calibrated by making a trial run on some premeasured area and measuring the output. For example, using a hand-held sprayer, spray a premeasured test area with water using the same pressure and techniques (i.e., travel speed and equipment) you would use when applying the pesticide. After spraying the test area, determine how much water was used. This volume can then be used to calculate the amount of water and pesticide needed to cover the intended application area.

The time invested in calibrating your equipment is time well spent. Accurate calibration to determine the application rate under your operating conditions is important for cost, efficiency and safety.

The objective of correct pesticide application cannot be achieved without good quality Plant Protection Equipment. A sound designed machine shall be proficient as far as pesticide distribution and delivery to the target in minimum time with least wastages is concerned. Hence, the machines should be tested to ascertain that they are:

i) Common Maintenance

Clean outer surface with brush or cotton waste by using plenty of water or kerosene oil.

Oil the moving or rubbing surfaces of parts with lubricating oil or grease.

Filter the chemical solution/ fuel oil mixture while pouring into the tanks. Make the caps or lids leak-proof with gaskets.

Flush the equipment with clean water to wash inside parts of containers, tubes and nozzles to be free from chemicals.

Any sprayer nozzle essentially breaks the liquid into small droplets and throws them. All nozzles in the field produce rangesof droplets of different size called spray spectrum. The hydraulic nozzles have wide range of size of droplets whereas the rotary nozzles have narrow range. Adroplet of 400 µm diameter is 1000 times bigger in volume than a droplet of 40 µm. The volume of spherical droplets can be worked out as 4/3 µr3. So if the droplet size of aspraying system could be reduced to half, then there will be 8 fold increases in the number of droplets and the coverage will also increase.

Majority of the pesticides are applied as sprays. The liquid formulations of pesticide either diluted or directly are applied in small drops to the crop by different types of sprayers. Generally the wettable powder, EC formulations are diluted suitably with water which is a common carrier of pesticides. However, in few cases oil is used as carrier of pesticides.

Spraying nozzle is a device for emitting spray liquid, breaking it up into small droplets and throwing the droplets away from the nozzle orifice. Different designs of nozzle are used to produce appropriate droplet size spectrum. In order to break the liquid into droplets energy is needed. The spray nozzles therefore are classified as:

Pesticide Dusting

The dusting powders are low concentration ready to use type, dry formulations containing 2 to 10% pesticide. The inert material or dry diluents is talc, soapstone, attapulgite, etc. and it is non toxic. The sulphur dust is not diluted with inert material.

Sprayer is an equipment in which the spray fluid is broken up into fine droplets and ejected under pressure. Actually, the function of the sprayer is to provide the spray fluid with some energy so that it can be broken into fine droplets through the nozzle and propelled to the target. The energy can be produced manually or with the help of any other source of power. There are a variety of sprayers.

Type of Sprayers: There are a variety of sprayers.

The Effectiveness of a Pesticide in Controlling a Disease and Pest Depends on

Using the right pesticide for the target disease & pest.

Applying the pesticide at the right time.

Applying the pesticide in the right amount.

Applying the pesticide to the right place.

The behavior of pesticides is dependent on many factors, so be sure to consider everything from light and temperature to moisture and pH and recognize that all the factors will impact the active ingredient. Here is some information on these topics, and others, which will improve pest management success.

1.Identification: The first step to disease and pest management success is the proper identification of the insect, disease or weed pest. Without identification of the pest, controls may be misused or improperly timed.

2.Time of Application: Time of application of pesticides plays major role in optimizing pesticidal performance. Early morning or late afternoon are the best times to spray. Dust formulations should be apply in early morning hours (6 am).In the case of foliar spray, it should be done in morning hours between 8 am to 11 am. Avoid spraying during the heat of the day.

3.Water Quality and pH: Primary factors important in water quality are pH and alkalinity (bicarbonates). Most of the pesticides show high efficiency in slightly acidic to slightly basic water pH (i.e. 6.5 to 7.5). Many water sources in alkaline pH (<7.0). Alkali pH (7.5>) is always harmful for pesticides. High pH levels are responsible for alkaline hydrolysis, a process that breaks down the active ingredient in many insecticides, fungicides and herbicides.Add a buffer agent to lower spray solution to a pH of 5.5-6.5. This will give better initial knockdown and longer residual action.

Antibiotics are substances which are produced by micro-organisms and which act against micro-organisms. It is also defined as secondary metabolites produced by microorganism, which inhibits the growth of another microorganism. Most antibiotics known uptill now are products of actinomycetes and some are from fungi and bacteria. The use of the antibiotics in managing plant diseases, particlulaty the disease caused by bacteria, mycoplasma and ricketsia, is well demonstrated and are thought to be effective means because of their selective action directed towards causal agents not to the infected host. The mode of action of the antibiotics varies depending on site of action which in turn detrmines their specificity towards given target host (Table 12). Some time mixture of antibiotics are in practice in managing the diseases to reduce delay the chances of resistance development against a particular antibiotic by the causal agent. The most useful antibiotic mixture is streptocycline, which contains streptomycin and tetracycline.

1. Bordeaux Mixture

It was invented in the Bordeaux region of France by PM Millardet (1982).

Also called Bordo Mix.

Mixture of Copper sulfate (CuSO₄), Slaked lime and Water.

It is known locally in France as Bouillie Bordelaise.

Before applying fungicides in field at large areas, it is required to be thoroughly investigated under laboratory conditions for its efficacy on pathogen, subjected. Basic methods of testing these chemicals against subjected pathogen, under laboratory conditions are described here.

1. Poisoned Food Technique

Principle

The principle involved in this technique is to poison the medium with fungicides and then allow a teat fungus to grow on such medium. The test may be conducted both on solid and liquid medium.

Pesticides sustain food production and control disease and pests. They are vital for crop production and instrumental in continuous increase in food production. The consumption of pesticide in India is one of the lowest in the world. India uses a low amount of 0.5 kg/hectare pesticide compared to 7.0 kg/ hectare by USA, 2.5 kg/hectare by Europe, 12 kg/hectare by Japan and 6.6 kg/ hectare by Korea. However, despite the low consumption of pesticides, India has more problem of pesticide residues vis-a-vis other countries and these have entered into food products and underground water because of

Pesticide Toxicity

Chemical tactics to manage pests can include many types of compounds. Some merely repel or confuse pests. Some interfere with weed photosynthesis, insect molting processes or development in some way. Others, including some botanical and most conventional insecticides are broadly toxic to living systems. The term pesticide literally means “pest killer". Generally speaking, when using pesticides, we are trying to garner the advantages of using pesticides while minimizing the risk to humans and other non-target organisms. Risk to humans of pesticide use is often described as:

Definition

“Biological pesticides”, or “Biopesticides” as the name suggests, are naturally occurring substances that biologically control harmful pests, especially among field crops. These are naturally produced bio chemical materials basically non-toxic to the environment that can be employed in pest control. Biopesticides could mean living organisms (bacteria, virus, and algae), their products (biochemicals produced by them) and also plant byproducts. Biopesticides offer an ecologically effective solution to the harmful effects of synthetic pesticides.

Strobilurins are a group of chemical compounds used in agriculture as fungicides. They are part of the larger group of QoI inhibitors, which act to inhibit the respiratory chain at the level of Complex III.Some common Strobilurins are azoxystrobin, kresoxim-methyl, picoxystrobin, fluoxastrobin,oryzastrobin, dimoxystrobin, pyraclostrobin and trifloxystrobin. Strobilurins represented a major development in fungus-based fungicides. They were extracted from the fungus Strobilurus tenacellus. They have a suppressive effect on other fungi, reducing competition for nutrients; theyinhibit electron transfer inmitochondria,disrupting energy metabolism and preventing growth of the target fungi. Strobilurins are miracle fungicides ever developed with novel mode of action & mobility that qualifies the Unique New Generation Fungicides controlling Pathogen from diverse class.

Environmental protection Agency (EPA)has established category of pesticides called “Reduced Risk” pesticides to encourage the development, registration and use of products which could result in reduced risks to human health and the environment. New Generation pesticides are considered for “Reduced Risk” status if they have at least one or more of the following status: low risk to human health, low toxicity to non target organisms, low potential to contaminate ground water, surface water or other valued environmental resources and have the potential to expand the adoption and effectiveness of IPM.

There is a common practice of using the terms spurious, counterfeit, misbranded, illegal, sub-standard and others interchangeably and this often creates confusion among scientist, industrialist and extension officer. Spurious and counterfeit pesticides are the two major words used to describe all kind of anomalies in the pesticides. Hence, there is a need to have a clear understanding of these and differences between them.

Definition

Adjuvants are chemicals incorporated in a pesticide formulation or added to the tank mix, to increase effectiveness and safety. They generally have no effect on pests in themselves.

Pesticides can cause three types of harmful effects – Acute, Delayed and Allergic.

Acute effects: Are those that occur immediately after the exposure, within minutes or hours.

Pesticide poisoning occurs when a pesticide enters the body and interferes with the vital systems of the body. Symptoms can begin anything from 1/2 hour to 24 hours after exposure. The typical symptoms and signs of pesticide poisoning are:

At times a pesticide application fails to reduce the levels of a pest. Usually, the farmer blames either the pesticide for this or he thinks that the pests have developed resistance. Though, the usual causes of poor results with an application of a genuine pesticide from a reputable source are:

The pesticides-one amongst the most important inputs in the crop production contributed a lot to minimize the losses to crop yield. In India, on an average, 33 % of crop loss occurs due to pests and disease and runs to an estimated Rs 1.40000 crore. Pesticide is an essential in the farmers struggle to protect their crops. Pesticides consumption in India reported to 288g/ha is very low compared to global average of 900g/ha. Excessive, irrational and indiscriminate use of these pesticides causes many negative consequences, mainly the famous 3 R viz; Resistance, Resurgence and Residue aspects.

Pesticide Resistance

Resistance to pesticides may be defined as ‘a heritable change in the sensitivity of a pest population that is reflected in the repeated failure of a product to achieve the expected level of control when used according to the label recommendation for that pest species.

The label on the pesticide container is the principal source of information about a product. The label has all the fundamental information that is required. If you need information about a pesticide, do not rely on memory as it is very easy to make a mistake. Read the label every time before using a pesticide. The lay-out of the pesticide label varies between countries. However, every label on a pesticide container from a reputable manufacturer or source will have the following information:

Tank mixing two or more pesticides saves time and labor and reduces equipment and application costs. In some cases, however, it can alter the effectiveness of one or more of the products. Two or more pesticides are considered compatible when they can be mixed and applied in combination without adversely affecting the effectiveness or the physical and chemical properties of the mixture, or causing undesirable damage to the application site. When problems develop from mixing two or more products together, the chemicals are considered incompatible.

Incompatibility

Incompatibility is a condition that prevents pesticides from mixing together properly to form a uniform solution or suspension. The formation of flakes, crystals, or oily clumps, or severe separation is unacceptable. Such incompatible mixtures clog application equipment and limit even distribution of the active ingredient in the spray tank. This prevents good pesticide coverage. The cause of incompatibility may be the chemical nature of the materials you are mixing. Impurities in the spray tank or water also may affect compatibility. Even the order in which you mix pesticides in the spray tank is important. Sometimes the types of formulations being mixed influence compatibility. Pesticide formulations of the same type are rarely incompatible with one another because they usually contain many of the same inert ingredients and solvents. Incompatibility can be a matter of timing or placement of the pesticides. Chemical or physical incompatibility may occur between the pesticides.

When two or more chemicals can be used in combination or mixed safely, they are said to be compatible. These combinations can be a pesticide mixed with another non-pesticide chemical, or can be two or more pesticides that are combined in the same tank mix. The reasons for combining pesticides are:

1.To control different types of pests with a single application. Often, several types of pests need to be controlled at the same time. It is generally more economical to combine the pesticides needed and make a single application.

2.To provide superior control than that obtained from one pesticide. Applicators at times combine active pesticides to kill a pest that has not been effectively controlled by either chemical alone. Many combinations are quite effective, but in most cases it is not known if the improved control is a result of an additive effect or a synergistic action of the combined chemicals on different segments of the pest population. One should always check the label prior to verify the safety and legality of mixing pesticides.

3.To increase the effectiveness of one of the chemicals. As mentioned above, this is called synergism. The material added to increase the effectiveness of the primary chemical is called a synergist. The synergist may not necessarily be pesticidal by itself, but increases the effectiveness of the pesticide with which it is combined.

Pesticide Formulation

Pesticide formulation is the process by which the pesticide is put into a form which can be easily produced, stored, transported and applied by practical methods in order to achieve a safe, convenient economic and effective method of pest control.

When a pesticide active ingredient (a.i) is manufactured, it is not in a usable form as it may not mix well with water or may be unstable. Therefore, it is mixed with other compounds to improve its effectiveness, safety, handling and storage. These other compounds can include solvents, mineral clays, stickers, wetting agents, or other adjuvant. The mixture of a.i. and inert (inactive) ingredients is called a pesticide formulation. Some formulations are premixed while others must be mixed before use. A single a.i. is often made into several formulations.

The Insecticides Act, 1968 and Insecticides Rules, 1971 regulate the import, registration process, manufacture, sale, transport, distribution and use of pesticides with a view to prevent risk to human beings or animals and for all connected matters, throughout India. However, recently the Union Cabinet gave its approval for the introduction of the Pesticides Management Bill with the aims of improving the quality of pesticides available to Indian farmers and introducing new, safe and efficacious pesticides. This bill will replace the existing Insecticide Act 1968.The pesticides’ regulations in India are governed by two different bodies:

There are broadly five categories of crop protection products

Insecticides: Insecticides provide protection to the crops from the insects by either killing them or by preventing their attack. They can be further classified based on their mode of action:

Advantages of Using Pesticides

Pest are an ecological problem and therefore our control strategy must be ecologically sound. Modern agriculture is a combination of both and human intervention is necessary, whether it be pulling out weeds by hand, use of pesticides or genetic engineering. Control methods evolve over time as knowledge and techniques improve. This includes the development of chemical means of control which become very important because of a number of advantages

Pest are an ecological problem and therefore our control strategy must be ecologically sound. Modern agriculture is a combination of both and human intervention is necessary, whether it be pulling out weeds by hand, use of pesticides or genetic engineering. Control methods evolve over time as knowledge and techniques improve. This includes the development of chemical means of control which become very important because of a number of advantages

A pesticide may be referred to by several different names, which can cause confusion. The same pesticide may be referred to by a number of different names, or different pesticides may have similar names.

A pesticide is any material intended to prevent, destroy, repel, or mitigate any pest, which is defined as a living organism that occurs where it is not wanted or that causes damage to crops, humans, or other animals. There are many different types of pesticides, each is meant to be effective against specific pests. The term “-cide” comes from the Latin word “to kill.”

Pesticides offer one of the best means of controlling plant diseases and pests and subsequently improving the yield. They have been used since the 19^th century. Pesticides, undoutly are poisonous and their indiscriminate use and abuse may result in ecological imbalance resulting in pest resurgence, aggravation of minor pest, pesticides resistance, environmental pollution, poising serious health hazards to man and animals. However, if used judiciously, following the guide line of Pesticide Action Network (PAN), such problems are not likely to occur, and instead improve crop yield. It is however unfortunate that many of us really lack the insight in Materia Medica of plant diseases and pests and most of the problems have occurred due to misuse and abuse of pesticides for which man is responsible and not the pesticides.

Seed marketing is one of the most vital components of seed technology. Broadly, it includes such activities as production, processing, storage, quality control and marketing of seeds. In the narrow sense, however seed marketing refers to the actual acquisition and selling of packed seeds, intermediate storage, delivery and sales promotional activities. Seed marketing comprises the following:

1. Demand forecasts (assessment of effective demand)
2. Marketing structure
3. Arrangements for storage of seeds
4. Sales promotional activities
5. Post-sales service
6. Economics of seed production and seed pricing

A bill to provide for regulating the quality of seeds for sale, import and export and to facilitate production and supply of seeds of quality and for matters connected therewith or incidental thereto. Be it enacted by Parliament in the Fifty-Fifth Year of the Republic of India as follows

Aims and Objectives

It has become evident that in order to achieve the food production targets of the future, a major effort will be required to enhance the seed replacement rates of various crops. This would require a major increase in the production of quality seeds, in which the private sector is expected to play a major role. At the same time, private and Public Sector Seed Organisations at both Central and State levels, will be expected to adopt economic pricing policies which would seek to realise the true cost of production. The creation of a facilitative climate for growth of a competitive and localised seed industry, encouragement of import of useful germplasm, and boosting of exports are core elements of the agricultural strategy of the new millennium.

Biotechnology will be a key factor in agricultural development in the coming decades. Genetic engineering/modification techniques hold enormous promise in developing crop varieties with a higher level of tolerance to biotic and abiotic stresses. A conducive atmosphere for application of frontier sciences in varietal development and for enhanced investments in research and development is a pressing requirement. At the same time, concerns relating to possible harm to human and animal health and bio-safety, as well as interests of farmers, must be addressed.

Globalization and economic liberalization have opened up new opportunities as well as challenges. The main objectives of the National Seeds Policy, therefore, are the provision of an appropriate climate for the seed industry to utilize available and prospective opportunities, safeguarding of the interests of Indian farmers and the conservation of agro-biodiversity. While unnecessary regulation needs to be dismantled, it must be ensured that gullible farmers are not exploited by unscrupulous elements. A regulatory system of a new genre is, therefore, needed, which will encompass quality assurance mechanisms coupled with facilitation of a vibrant and responsible seed industry.

Preliminary

1. Short title and extent

1. This Order may be called the Seeds (Control) Order, 1983
2. It extends to the whole of India
3. It shall come into force on the 30th December, 1983

Part I - Preliminary

1. Short title: These rules may be called the Seeds Rules, 1968
2. Definitions: In these rules, unless the contest otherwise requires.

An Act to provide for regulating the quality of certain seeds for sale, and for matters connected therewith. It enacted by Parliament in the Seventeenth Year of the Republic of India.

1. Short Title, Extent and Commencement

• This Act may be called the Seeds Act, 1966.
• It extends to the whole of India.
• It shall come into force on such date as the Central Government may, by notification in the Official Gazette, appoint, and different dates may be appointed for different provisions of this Act, and for different States or for different areas thereof.

Nature has bestowed our country with diverse agro climatic and topographic conditions ranging from temperate to humid and from sea bed to snow line. The existing climatic conditions with a plenty of sunshine, nutrient rich soil, abundant water resources and reservoir of trained manpower, enable our country to grow various types of vegetables in tropical, subtropical, temperate, alpine and desert vegetation zones in one or the other seasons during the year.

Vegetables, the cheapest and nutrient-rich food source within the economic reach of poor man, play a vital role in human diet since they provide carbohydrates, protein, fat, minerals, vitamins, fibers and phytochemicals (non-nutrient bioactive compounds), which are essential for making the body immune system strong, detoxifying carcinogens, reducing muscular degeneration and protecting the body from infectious ailments. Vegetables besides having medicinal values also play an important role in nutritional security and national economy of the country since they are quick growing and short duration, and their yield per unit area per unit time is 5-10 times higher than the cereal crops.

Computer played a distinctive role in the growth of most advanced, precise and highly sensitive instruments especially in medical and analytical instrumentation technology. Due to the development of different kinds of software the instrumentation analysis has become easy, rapid and result oriented. Auto sampling, development of new methods for detection analysis and the validity of new developed method have been possible with no trouble. Once the standardization of method is over and saved, it can be applied at any time to integrate the unknown samples and their data calculation. Thus, it avoids repetition in calculation and the end result is time saving technique due to the development of computer-organized system in analysis.

The results produced are more reliable and reproducible through various computer based instruments e.g. scanning machines, blood analyzer, gas chromatography (GC), amino acid analyzer (AAA), atomic absorption spectrophotometer (AAS) and high performance liquid chromatography (HPLC) etc.

HPLC is a technology or a system that works on the basis of software. There have been many definitions of computer shielded HPLC, such as high performance liquid chromatography (HPLC), high-pressure liquid chromatography (HPLC), high-speed liquid chromatography (HSLC), high efficiency liquid chromatography (HELC), and simply liquid chromatography (LC).

Chromatography was first employed by Ramsay (1905) to separate mixtures of gases and vapours. The first scientist to recognize chromatography as an efficient method of separation was the Russian botanist Tswett (1906), who demonstrated that, when a plant extract was carried by petroleum ether through a column consisting of a glass tube packed with calcium carbonate powder, a number of dyes were separated, as shown in Figure 1. He named this analysis method “Chromatographie” after “chroma” and “graphos”, which are Greek words meaning “color” and “to draw,” respectively.