eChapters

A. Answer the following

Q.1.    What is the difference between apoptosis and necrosis?

Ans.    In apoptosis, there is pronounced decrease in cell volume, modification of cytoskeleton, condensation of chromatin and degradation of DNA into fragments. Due to these changes, an apoptotic cell sheds tiny membrane bound apoptotic bodies containing intact organelles. Macrophages phagocytose apoptotic bodies and cells in the advanced stages of apoptosis. But there is no release of proteolytic and the lytic enzyme, cationic proteins and oxidizing molecule in the surrounding tissue and no local inflammatory response. On the other hand, in necrosis cells swell and burst releasing their contents and possibly triggering a damaging inflammatory response.

Predominantly, an agrarian society is mostly dependent on the farm sector. Agriculture plays an important role in Indian economy notwithstanding its shrinkage in share in the gross domestic product (GDP) of the country being 55.4% in 1950-’51 and 17.4% in 2018-’19 (Tripathy, 2019). Still the agriculture and allied sector is providing employment to 54 percent of the Indian population.

1.1 Introduction

Poultry is one of the fastest-growing areas of India’s agricultural industry, with an annual growth rate of around eight percent. Over a span of four decades, the chicken industry in India has seen a paradigm shift in structure and operation, transforming from a simple backyard occupation to a significant commercial agri-based enterprise. Constant efforts in upgrading, modifying, and using new technologies opened the path for the multiplication and diversification of poultry industries. The expansion is not just quantitative, but also qualitative, sophisticated, and productive. This transition has necessitated substantial expenditures and increases in breeding, hatching, raising, and processing. The rise of the poultry industry in India is also characterised by the expansion of chicken farms.

To fully explore the history of medical and drug development, it would be necessary to go back as early as 13,000 BCE, where drawings on the Lascaux caves in France suggest that humans were using plants for medicinal purposes. Since then, medical traditions have evolved and dissolved across societies all over the globe. Natural products including plants, animals and minerals have been the basis of treatment of human diseases. The last two centuries have seen a focus on scientific medicine, with a focus on drugs whose effects that have been proved by the scientific method. Yet given this setting, many of the major pharmaceutical corporations have renewed their strategies in favor of natural product drug discovery. Numerous drugs have entered the international pharmacopoeia via the study of ethnopharmacology and traditional medicine. At this point in history, it is possible for both traditional and western medicine to benefit from collaboration between the two disciplines. For traditional medicines, newer guidelines of standardization, manufacture and quality control and scientifically rigorous research on the scientific basis for traditional treatments will be required. In return, traditional medical traditions can offer a more holistic approach to drug design and myriad possible targets for scientific analysis. Powerful new technologies such as automated separation techniques, high-throughput screening and combinatorial chemistry are revolutionizing drug discovery. Traditional knowledge can serve as powerful search engine, which will greatly facilitate intentional, focused and safe natural product drug discovery and help to rediscover the drug discovery process. By looking at the historical trends in drug and medical developments, it is possible to understand how current drug development will benefit from this partnership.

Metagenomics is a relatively new field that combines molecular biology and genetics in an attempt to identify, and characterize the genetic material from environmental samples and apply that knowledge. The genetic diversity is assessed by isolation of DNA followed by direct cloning of functional genes from the environmental sample. But more importantly, it is extremely beneficial in the study of synthetically made genomes. Metagenomics is described as “the comprehensive study of nucleotide sequence, structure, regulation, and function”. Scientists can study the smallest component of an environmental system by extracting DNA from organisms in the system and inserting it into a synthetic model organism. The model organism then expresses this DNA where it can be studied using standard laboratory techniques. The development of metagenomics stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent thevast majority of organisms in most environments on earth. This evidence was derived from analyses of 16S rRNA gene sequences amplified directly from the environment, an approach that avoided the bias imposed by culturing and led to the discovery of vast new lineages of microbial life.

Metagenomics is employed as a means of systematically investigating, classifying, and manipulating the entire genetic material isolated from environmental samples. This is a multi-step process that relies on the efficiency of four general steps as shown in the diagram. The procedure consists of: the isolation of genetic material, the manipulation of the genetic material, library construction, and the analysis of genetic material in the metagenomic library. Metagenomics provides a second tier of technical innovation that facilitates study of the physiology and ecology of environmental microorganisms. Novel genes and gene products discovered through metagenomics include the first bacteriorhodopsin of bacterial origin; novel small molecules with antimicrobial activity; and new members of families of known proteins, such as an Na⁺(Li⁺)/H⁺ ant porter, RecA, DNA polymerase, and antibiotic resistance determinants. Reassemblyof multiple genomes has provided insight into energy and nutrient cycling within the community, genome structure, gene function, population genetics and micro heterogeneity, and lateral gene transfer among members of an uncultured community. The application of metagenomic sequence information will facilitate the design of better culturing strategies to link genomic analysis with pure culture studies.

Introduction There is an emergence of a transformative paradigm of participatory approaches in various fields such as management, health care and education, community development, etc. Participatory approaches challenge the top-down approach used frequently for decision making (Narayanasamy, 2009). Participation is a dynamic process where two individuals exchange their ideas, thoughts, and knowledge for making proper decisions and solving the problems faced by local people. It is a process of empowering individuals that helps people make proper analysis, make their own decisions, and develop their confidence. It is successfully used in increasing self-help activities of local people and for proper implementation and evaluation of large scale projects. FAO has reported that through participatory programmes it is very easy to mobilise local knowledge for self-reliant development, reducing government assistance costs.

Botanically fruits are ripened ovary. It is a plant part that may or may not be purely derived from ovary of the flowers may be fleshy or pulpy in character, often juicy, predominantly sweet. With somewhat acidic taste, fragrance, aromatic flavours, fruits are generally not consumed during principal part of meals. Whereas vegetable is plant part which is less sweet, generally non acidic and consumed either cooked or raw during the principal part of our main meals. Vegetables may be fruit, root, stem flower, or leaf part of a plant or their modification.
Fruits and vegetables are valued in in human diet as an important source of protective nutrients like Minerals and vitamins. Apart from their nutritive value they add variety to the diet and improve acceptability of meal due to presence of different shades of colours, flavours, and texture. However, the major role in food preservation is played by their moisture content. For the same reason they are classified as
1. Perishable: Those which deteriorate quickly after harvesting such as tomatoes, mangoes, papaya, peaches, plums, juicy fruits and vegetables. They have high degree of moisture content and are highly susceptible to spoilage.
2. Semi perishable: These have less moisture content as compared to perishable one’s example 30 carrots, peace, green beans, apple etc. These foods are containing certain natural inhibitors to spoilage.

What is seed?

“A seed is an embryo, a living organism securely fixed in the supporting or the food storage tissue.”

A true seed is defined as “Structurally a true seed is a fertilized matured ovule, consisting of an embryonic plant, a store of food and a protective seed coat, a store of food consists of cotyledons and endosperm”. In broad sense, “seed is a material which is used for planting or regeneration purpose”. Scientifically, “seed is a fertilized matured ovule together covered with seed coat is called seed” or “it is a propagating material i.e., part of agriculture, sericulture, silviculture and horticultural plants used for sowing or planting purpose”.

The business of Seed Technology is to protect this biological entity and look after its ‘welfare’, while the focus of Food Technology is on the second component – the supporting tissue.

Introduction Surimi and surimi based seafoods are traditional products of Japan and occupy an important position in the dietary culture of the country. Today, the largest producers of surimi are the United States, Japan and Thailand. Surimi is also manufactured in China, Vietnam and Malaysia. The process of makingsurimi originated in Southeast Asia and was further developed in Japan in the 16th century. Surimi is a product of Japanese origin, derived from a traditional Japanese way of using and preserving fresh fish. The word ‘surimi’ comes from the Japanese words ‘suru’ meaning ‘to process’ and ‘mash/mi’ meaning ‘meat’. Technically, surimi is the stabilized myofibrillar protein which is obtained by mechanically deboned fish flesh, which is washed, mixed with cryoprotectants, and stored frozen. Washing not only removes fat and undesirable matters such as blood, pigments and odoriferous substances but also increases the concentration of myofibrillar protein. A fish-based product serving the raw material for preparation of analog of seafoods like crab, lobster, scallop & other shellfish.

Thermochemical conversion is a process that uses heat to transform biomass and agricultural waste into energy and valuable byproducts. This approach involves the decomposition of organic materials under high temperatures, with or without the presence of oxygen, to produce fuels, chemicals, and thermal energy. The key thermochemical methods include combustion, pyrolysis, gasification, torrefaction, and hydrothermal liquefaction (HTL). In the pursuit of sustainable energy and environmental protection, biomass has emerged as a vital renewable resource. Thermochemical conversion technologies have gained prominence due to their high efficiency, rapid processing rates, and ability to handle a wide range of feedstocks—including lignocellulosic and low-moisture materials. Unlike biological processes, which depend on microbial activity and require longer processing times, thermochemical methods offer faster and scalable solutions suitable for industrial applications. Each thermochemical pathway offers unique advantages and outputs. Combustion is the most widely used method, involving the burning of biomass in the presence of excess oxygen to generate heat. This heat can be used directly or converted into electricity, making combustion especially common in rural and semi-urban settings. Other methods like pyrolysis and gasification produce intermediate products such as bio-oil, syngas, and biochar, which can be further refined or utilized as fuels and soil amendments. Hydrothermal liquefaction and torrefaction operate under specific conditions to produce high-energy-density products and improve biomass handling and storage characteristics. Overall, thermochemical conversion presents a robust strategy for managing agricultural waste while contributing to renewable energy generation and circular economy goals.
Pyrolysis, Gasification, and Hydrothermal Processing
Pyrolysis is a thermochemical process that involves the decomposition of biomass by heating it in the absence of oxygen, typically at temperatures between 300°C and 700°C. This process converts organic material into three main products:
1. Bio-oil (liquid)
2. Syngas (gas)
3. Biochar (solid)

Introduction

Parvoviruses are nonenveloped, single-stranded DNA viruses belonging to the genus Protoparvovirus, member of the Parvoviridae family, that has been included within the species Carnivore protoparvovirus 1, together with Feline panleukopenia virus (FPV), Mink enteritis virus (MEV) and Raccoon parvovirus (RPV), according to the International Committee on Taxonomy of Viruses (Tijssenet al 2011). Two distinct parvoviruses are known to infect dogs—the pathogenic CPV 2 and CPV-1 or the minute virus of canine (MVC). CPV-2, the causative agent of acute hemorrhagic enteritis and myocarditis in dogs, is one of the most important pathogenic viruses with high morbidity (100%) and frequent mortality up to 10% in adult dogs and 91% in pups. CPV-2 is the etiologic agent of a contagious disease, mainly characterized by clinical gastroenteritis signs in younger dogs. This virus that causes the disease “parvo”, first emerged among dogs in Europe around 1976. By 1978 the virus had spread unchecked, causing a worldwide epidemic of myocarditis and inflammation in the intestines (gastroenteritis) and could infect domestic dogs, spreading in the global dog population within 2 years. This original type, was rapidly replaced by two antigenic variants, types 2a and 2b (Parrish et al 1985, 1991), and in 2000 a third type CPV-2c was detected (Buonavogliaet al 2001). Canine parvovirus (CPV) is responsible for acute gastroenteritis in pups, with a high rate of mortality (Carmichael and Binn, 1981). We now know the virus is not limited to dogs, but is capable of causing infections in wild canines such as coyotes and wolves, and other wild animals, including foxes, raccoons and skunks. Since its emergence in 1978, it was and remains a common and important cause of morbidity and mortality in young dogs.

Introduction

The food problem in the world is expected to become more serious if the world population keeps growing at the present rate. Out of various strategies to solve the problem, the post-harvest handling of the grains is of paramount importance. Tribolium species is major pest of stored grain and grain products in the tropics. Control of this insect relies heavily on the use of synthetic insecticides, which has led to many problems such as increasing costs of application, pest resurgence, pest resistance to pesticides and lethal effects on non-target organisms in addition to direct toxicity to users and disturbances of the environment. Thus, insecticides of natural origin are rational alternatives of synthetic insecticides. Several botanicals, natural products and their derivatives have been reported and exhibit better efficacy and potency against the stored grain pests.

In the present investigation, an attempt has also made to test ovicidal, larvicidal and adult emergence suppression potentials of the extracts of some of the locally available Indian medicinal plant viz., Datura stramonium, Ricinus communis, Vitex trifolia and Nerium indicum against T. castaneum against T. castaneum, commonly called as Red flour beetle.

ABSTRACT

Papaya (Carica papaya L.) is an important crop in the tropics and subtropics for export of fruits and above all, for local consumption. Cultivation of papaya faces with problems due to its heterozygosity, dioecious nature and susceptibility to a large number of viral diseases. Crossing between two hermaphrodites will produce the highest ratio of fruit bearing female trees, which is of homozygous dominant andromonoecious types which are always letha l due to po st fertilization barriers leading to their abortion. The gynodioecious varieties of papaya have the M1M1 forms while selfing and hence for explant selection , gynodioecious varieties like Sunrise solo and CO7 were prefered Embryo culture is one of the earliest forms of in vitro culture applied to solve practical problems and is probably the tissue culture techniques that has proven of greatest value to breeders. Ovule culture is practised in a case when small (or) young embryos that abort at early stages of development and are difficult to isolate. This necessiates the development of a protocol for initiation, multiplication and regeneration of ovular callus of papaya.

? Oxalic acid is an organic dicarboxylic acid that readily forms insoluble salts with cations like calcium & magnesium.

? Sodium, potassium or ammonium salts are highly soluble and can induce toxicity in animals.

Source

? Ingestion of large quantity of oxalate containing plants.

? For toxicity plant should contain > 10% oxalic acid on dry weight basis.

? The form in which oxalate is present in plant mainly as acid oxalate (Oxalis sp., Rumex sp.) or oxalate ions (chenopodiacae).

Method I
Reagents
    1.    20% sodium carbonate.
    2.    Conc. hydrochloric acid.
    3.    4% sodium hydroxide
    4.    10% calcium chloride
    5.    Ammonium hydroxide
    6.    Potassium permanganate (0.05 N)

Procedure
Take 2-5 g of powdered sample in a beaker without spout, and add 40 ml of water and boil for half an hour. In case of faeces, add 40 ml of 4 percent NaOH instead of 40 ml of water to form silicates. Now boil, for half an hour with cold water flask over the beaker, then add 10 ml of 20 percent sodium carbonate solution and boil for  another half an hour. During these two cooking processes, the following changes take place.

7.1 INTRODUCTION

An understanding of the processes of oxidation and reduction in soils and the associated changes and practical implications requires a knowledge firstly of soil respiration and the movement of O₂ and CO₂ in soils, and secondly an appreciation of the significance of the changes which occur when, as a result of the use of all the O₂ in a given zone of the soil, respiration changes from aerobic to anaerobic. This results in marked changes in the biochemistry of the system. A basic knowledge is therefore needed of the microbiology and biochemistry of aerobic and anaerobic respiration and of the physics of gas movement, as well as of the chemistry of the system.

Introduction

Lipids are among the major components of food of plant and animal origin (food lipid). There is no precise definition available for the term lipid; however, it always includes a broad category of compounds that have some common properties and compositional similarities. Lipids are materials that are sparingly soluble or insoluble in water, but soluble in selected organic solvents like benzene, chloroform, ether, hexane, and methanol. Together with carbohydrates and proteins, lipids constitute the principal structural components of tissues (Erwin Wsowicz et al., 2004). Lipid oxidation is one of the major reasons that foods deteriorate and is caused by the reaction of fats and oils with molecular oxygen resulting in off-flavours that are generally called rancidity. Exposure to light, pro-oxidants and elevated temperature will accelerate the reaction (Velasco, J et al., 2010). Rancidity is related to characteristic off-flavour and odour of the oil. There are two major causes of rancidity. One occurs when oil reacts with oxygen and is called oxidative rancidity (Ribeiro et. al., 1993). The other cause of rancidity is by a combination of enzymes and moisture. Enzymes like lipases liberate fatty acids from the triglyceride to make di and monoglycerides and free fatty acids and such liberation of free fatty acids is named hydrolysis. Hydrolysis is additionally caused by chemical process that’s prompted by factors like heat or presence of water. Rancidity caused by hydrolysis is called hydrolytic rancidity. Oxidation cares mainly with the unsaturated fatty acids. Oxidative rancidity is off interest because it results in the event of unfavourable off-flavours which will be detected early within the development of rancidity, more so than in the case of hydrolytic rancidity. Oxidation can alter the flavour and nutritional quality of foods and produce toxic compounds, all of which may make the foods less acceptable or unacceptable to consumers. Oxidation products typically include low relative molecular mass compounds that are volatile also as undesirable off-flavour compounds (Food lipids).

Introduction

The present trend in the search for new drugs is to look at natural sources, which are safer for humans and ecofriendly. Of late there has been increased awareness about the detrimental effects of synthetic food supplements, food additives and drugs, and the search for natural alternatives in plants has intensified. In this regard, horticultural crops are particularly appealing since they are not only edible, but also have diverse nutraceutical effects. Nutraceuticals (often referred to as phytochemicals or functional foods) are natural, bioactive chemical compounds that have health promoting, disease preventing or medicinal properties (http://foodsci.rutgers.edu),such as antioxidant, anti-inflammatory, hypoglycemic, hypolipidemic, antimicrobial effects, to name just a few. Most of these properties are attributed to the secondary metabolites present in plants, various phytochemicals including flavonoids, terpenoid, lignans, sulfides, polyphenolics, carotenoids, coumarins, saponins, plant sterols, curcumins, phthalides etc. The medicinal value of our horticultural crops is not a new knowledge, it has been exploited by the traditional systems of medicines like Ayurveda, folklore medicines and ‘kitchen remedies’ since time immemorial.

A functional food is similar in appearance to, or may be, a conventional food that is consumed as part of a usual diet, and is demonstrated to have physiological benefits and/or reduce the risk of chronic disease beyond basic nutritional functions, i.e. they contain bioactive compound.

A nutraceutical is a product isolated or purified from foods that is generally sold in medicinal forms not usually associated with foods. A nutraceutical is demonstrated to have a physiological benefit or provide protection against chronic disease.

2.1. Introduction
The present century is the century of oxidative stress (OS) that linked to several life-threatening disorders which predominantly affect public health as well as their nutritional status. Unhealthy lifestyle and eating pattern which is major concern for OS. The biological literature first used the phrase “oxidative stress (OS)” in 1970. OS can be defined as a state of imbalance condition between the high level of pro-oxidants and the low level of antioxidant scavenging capacity in the body. The normal oxidative process that occurs in cells is crucial for the existence of life and favours the death of a cell. Free radicals, which are unstable, can form when molecular oxygen breaks up into pairs. This unstable radical has a high level of reactivity and produces reactive molecular species like reactive oxygen species (ROS) and reactive nitrogen species (RNS). To resist the OS and to maintain the redox balance, cells have an inbuilt antioxidant defence capacity, comprising of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPOD) etc. But some pathogenic or environmental conditions may overpower this antioxidant defence system, allowing a portion of reactive oxygen and nitrogen species (RONS) to bypass degradation and create much more reactive radicals. Increased ROS and RNS affect redox balance and damage DNA, proteins, amino acids, and fats, causing alteration of structures as well as action of cells and tissues. This will ultimately produce several pathogenetic elements associated with clinical situations and leads to the development of human aging and numerous diseases. Several dietary components such as whole grains, nuts, fruits, and vegetables are excellent sources of antioxidative vitamins, minerals, nutraceuticals or phytoceuticals that shields the human body from the harmful oxidative injury by scavenging or destruction of free radicals and also by upregulation of endogenous antioxidant defence system. Thereby long-term and sustainable OS remedies can be performed.

7.1. INTRODUCTION

Abiotic stress is a major limiting factor in crop productivity. Several stresses, such as high temperature, high light intensity, osmotic stress, heavy metals, and several herbicides and toxin lead to over production of reactive oxygen species (ROS), causing extensive cellular damage and inhibition of photosynthesis (Allen, 1997). ROS is produced in both unstressed and stressed cells. Plants have well developed mechanisms against ROS, involving both limiting the formation of ROS as well as instituting its removal. Under unstressed conditions, the formation and removal of O₂ are balance. Introduction of molecular oxygen (O₂) into atmosphere by O₂- evolving photosynthetic organisms, ROS has been the unwelcome companion of anaerobic metabolism. In contrast to O₂, these partially reduced or activated derivatives of oxygen (O₂¹, O2-, H₂O₂, and HO) are highly reactive and toxic and can lead to the oxidative destruction in tissues during stress.

Abstract

Cyanobacteria  evolved during  the  Precambrian  era and are believed to be the first  one  to  introduce oxygen  into  the  initially  reducing  atmosphere  by  oxygenic  photosynthesis. Thus being the main primary producers of organic matter at that time and thereby contributed to a major global transformation leading to the development of aerobic metabolism and the subsequent rise of higher plants and animal forms. Reactive  oxygen  species  (ROS)  in  cyanobacteria  are  commonly  generated during  normal  growth  and  development.  However  abiotic  and  biotic stresses  enhance  the  level  of   ROS  which  in  turn  pose  the  danger  of oxidative  stress.  Ability  to  perceive  ROS  and  to  rapidly  initiate  antioxidant defenses  is  crucial  for  their  survival.  Therefore cyanobacteria evolved four lines of defense mechanisms to combat the damages caused by oxidative stress. Inretrospect  to  their  ecological  adaptivity, ubiquitous  presence  and  efficient  source  of   compounds  of biotechnological  significance, this chapter aims to elucidate various mechanisms adopted by cyanobacteria to combat oxidative stress.

During photochemical reactions of photosynthesis, takings place in the chloroplast lamellas system, electrons flow from water to NADP resulting in generation of a powerful reductant NADPH. Simultaneously, water is oxidised and O₂ oxygen is released. This reaction is known as Hills reaction. The rate of production of NADPH and ATP in the photochemical reaction is directly associated with the oxidation of water the sole electron donor. So the photochemical efficiency can be measured by measuring the rate of oxygen evolution.

Since oxygen evolution is a good reflection of the photochemical activity of chloroplast, determination of the rate of oxygen evolution can be conveniently adopted to estimate the photochemical activities. Normally oxygen evolution as a measure of photochemical reactions, is measured either in isolated chloroplasts or in the excised leaf discs using an oxygen electrode. An oxygen electrode is a special form of chemical electrode cell in which the current generated is proportional to the activity of oxygen present. This equipment is widely used for determining the amount of oxygen liberated during the photochemical reaction either in isolated chloroplasts or in leaf bits. Though oxygen electrode is the most frequently used technique to determine the rate of oxygen evolution, there are a few indirect methods to quantify the amount of oxygen evolved.

In photosynthesising tissues or in isolated chloroplasts, the rate of oxygen liberation can be determined by using appropriate artificial electron acceptors and donors (artificial electron donor and acceptors are compounds which can substitute the physiological electron transport carriers and helps in understanding the electron transport mechanisms and ATP synthesis). Oxygen is a powerful oxidizing agent which means it readily oxidizes another compound and itself gets reduced to water. This property can be effectively used in qualitative and quantitative determination of oxygen evolution in green leaves exposed to light. The oxygen that is liberated during the photochemical reactions can be determined by using an appropriate organic substance which can be readily oxidized by oxygen.

Oxygen therapy has been in vogue for more than century and it is key component in cardiorespiratory care both in human and veterinary medicine. It is recommended in all hypoxemic humans, dogs and cats (peripheral oxygen saturation < 90% to 94% or PaO2 < 70 mm Hg) presented with acute heart failure or respiratory diseases. But oxygen therapy in normoxemic subjects has remained controversial as some studies have shown detrimental effect (increased level of reactive oxygen species, oxidative stress, reduction in coronary blood flow and myocardial oxygen consumption) of oxygen therapy in subjects with normal oxygen level.

Introduction

Oxygen uptake of fish(s) displays its basal metabolic status and is taken into consideration as one of the signs of the overall fitness or/and well-being of the fish. It can also be beneficial to evaluate the physiological state of a living organism, facilitates in comparing the susceptibility or resistance potentiality and additionally beneficial to correlate the behaviour of the animal, which ultimately serve as predictors of functional disruptions of population. Analysis of Oxygen uptake machine to assess the primary harm or demand inflicted at the animal that can both boom or lower the oxygen uptake (Neelima et al, 2016).

The maximum vital single issue in transporting fish is imparting an adequate level of dissolved oxygen. Heavier fish transported in warmer water want more oxygen. For instance, if the water will increase by temperature 10°C (e.g., from 10 to 20°C), Oxygen uptake is roughly doubled.

Respiration of air-breathing fishes has fascinated researchers for a minimum of a century; the earliest attempts to measure oxygen uptake (V? O₂) and respiratory partitioning date to the 19th century (Baumert, 1853). The available general respirometry methods have been explained and reviewed by numerous authors (Cech, 1990; Lighton, 2008; Lefevre et al, 2016; Zhang et al, 2019), and researches of the respiration of air-breathing fishes had been reviewed in element within side the iconic book by Graham (1997). Air-breathing fish are generally characterized by a significant reduction in gill surface area with a purpose to keep away loss of oxygen taken up in the air-breathing organ (ABO) at the site of the gills in hypoxic water (Scott et al, 2017; Pelster et al, 2020).

ABSTRACT

The food industry has many the environmental challenges in terms of emerging microbial strains, bacteria, viruses and accumulation of toxic chemicals. Ozone (O₃), environmental bluish gas is an extremely potent oxidant, which is effective solution for disinfection, sanitization for various kinds of products. The application of ozone treatment in agro processing has become green technology with increasing acceptance. It can apply in liquid form, gaseous form to sanitize food, food packaging materials, process water and to disinfect equipments. Ozone can be generated by different methods such as ultraviolet radiation method, corona discharge method etc. In this chapter, ozone properties, its generation, antimicrobial power and advantages with application of ozone has covered. Fruits and vegetables treatment with ozone have shown increased shelf-life and many hazardless advantages of the products. Some products preserved with ozone. Anti microbial activity, no residues in foods, assurance about preservation of sensory, nutritional and physicochemical properties of food have made ozone application as a promising technology for all kinds of food products

1. Introduction

Therapeutic plants have different metabolites in them because of which they are generally utilized in the whole world by individuals to fix different illnesses. India has a rich history of involving many plants for therapeutic purposes. Therapeutic plants are assuming an exceptionally dynamic part in conventional medications for the treatment of different afflictions. India is a rich wellspring of restorative plants because of the varieties in soil, their heights, and other ecological and geological conditions. India’s utilization of plants for medical services goes back nearly 5000 years, when Ayurveda systematized around 8000 natural cures by utilizing plants. Root and tuber crops possess a striking situation in the food security of the creating scene because of their high caloric worth and sugar content. Some of them are as of now under development; however different species are developing wild as a dismissed gathering of financial plants. A portion of the tuberous species is significant due to their restorative as well as modern application. 

P680: Reaction center chlorophyll in Photosystem II (PSII) which absorbs maximum light of 680 nm in its neutral state.

P700: Reaction center chlorophyll in Photosystem I (PSI) which absorbs maximum light of 700 nm in its neutral state.

P870: Form of bacteriochlorophyll in reaction center of pigment system in photosynthetic purple bacteria that absorbs light maximally at 870 nm in its neutral state.

Paclobutrazol: It is a chemical compound which inhibits Gibberellin biosynthesis. It is also a growth retardant commercially used in overcoming alternate bearing of mango.

Palindrome: Inverted repeats of DNA such that the sequence is the same when read forward or backward; e.g., CAGTTGAC, or in the English language, “Madam, I’m Adam.”

Palisade Parenchyma : Palisade parenchyma cells are seen beneath the upper epidermis. It consists of vertically elongated cylindrical cells in one or more layers. These cells are compactly arranged without intercellular spaces. Palisade parenchyma cells contain more chloroplasts than the spongy parenchyma cells. The function of palisade parenchyma is photosynthesis.

Pantothenic acid (Vitamin B₅): An important constituent of coenzyme A which is involved in transacylation reactions.

Paeonia emodi Royle. (Paeoniaceae): Eng.- Himalayan Paeony; HindiUdsalap; Kash.- Mamekh [W]. A shurby perennial, found in W. Himalayas from Kashmir to Kumoan at 2400- 3300 m altitudes. Flowers yellowish white with a bunch of orange-yellow stamens.

P.C. Method of factor analysis: Principal-component’s method or simply P.C. Method of factor analysis, was developed by Hotelling. This \method searches to maximize the sum of squared loadings of each factor extracted. Accordingly P.C. factor determines more variance than would the loadings obtained from any other method of factoring.

Pack inspection: It is similar to continuous inspection except that here inspector(s) may make in-process checks during different processing operations under contract but not required to be present all the times in the plant during operation.

Package: The package means a pre packed box, tin, barrel, pouch or can etc in which food article is packed.

Package store: A store that sells bottled or canned alcoholic beverages for consumption off the premises.

Packaging: Packaging is an industrial and marketing technique for containing, protecting, identifying and facilitating the sale and distribution of agricultural, industrial and consumer products (Plate 467). Packaging is defined as an enclosure of products, items or package in a wrapped pouch, bags, box, cup, bottle or any container form to perform one or more of the functions as containment, protection and preservation, communication and utility or performance. Technically, packaging is a system or method by which a product from the manufacturing point to the consumer level reaches in a safe and sound condition at an affordable cost. The primary purpose of the packaging is to protect the food product, to keep it in good condition and to preserve the flavour until it reaches the consumer. It is therefore, very essential that a suitable form of the packaging is chosen for a finished product. Packaging is an art, which determines the buying decision of a consumer. or Wrapping / placement of objects in paper / other packaging materials, keeping in box, etc is known as packaging which has two main functions, to assemble the produce / objects in convenient units for handling and to protect them during transporting, marketing and storage which results in extended shelf life of the produce. “Packaging” play a very important role not only in ensuring safety of the product during transportation, storage and distribution and in conveying information about the pack contents to the consumers, but also ensuring desired sensory characteristics and micro biological soundless till consumed.

Packaging films: Packaging materials in the form of thin sheets which can be wrapped round a product. Films can be made from synthetic materials, such as plastics, or natural substances, such as whey proteins.
Packaging materials: Substances used to make packs. Packaging for foods is commonly made from a variety of materials, including glass, plastics, rubber, wood and paper, which are formed into a range of container types. The type of material chosen depends on the product to be packaged and the intended use.
Packaging: Enclosure or wrapping of products. Functions include product containment for handling, transportation and use, preservation, optimization of product presentation, hygiene and to facilitate product dispensing and use. The term covers retail (primary), grouped (secondary) and transport (tertiary) forms.
Packinghouses: Establishments in which products are packed.
Packs: Containers of varying shapes and sizes made from paper, plastics, cardboard or other materials that are used to enclose items such as food. The term is also used to describe items or groups of items which are packed in containers or enclosed in packaging materials.
Pakora: Indian snack foods consisting of pieces of spiced meat and/or vegetables enclosed in batters containing besan or chickpea flour and deep fried. Deep-fried and served hot with chutney. The fritters

Sugarcane is the most important sugar crop contributing more than 62% of the world's sugar production. With increasing human population in the world, sugar requirement has also gone up. Molasses is an important by-product of sugar industry. It is used for the production of alcohol by the distillaries. Alcohol serves as a base material in pharmaceuticals and several other industries. The manufacture of gur (jaggery) and khandsari as alternative sweetening agents in India from sugarcane also plays considerable role in the Indian economy, particularly in rural area. Nearly 40 % of the sugarcane produced is utilised for manufacture of gur and khandsari. Sugarcane is bound to play a greater role in the Indian economy in the years to come by offering a stable income to the farmers by way of cane price, by providing employment to rural masses, by realising excise duty, Cane Cess/ purchase tax and foreign exchange earning.

Acreage and production

Sugarcane is grown in tropical and sub tropical regions. Brazil has largest area and production of sugarcane.India is second to Brazil in sugarcane production. It is followed by Cuba and China. Other important cane growing countries are Pakistan, Mexico, South Africa, Australia, Indonesia etc. So far, as average sugarcane productivity is concerned Peru ranks first in average sugarcane yield. To meet the sugar requirement of increasing population in future, it is essential that sugarcane production is stepped up. Since there is no scope at present to bring additional land under sugarcane, productivity of sugarcane, the targets have to be achieved only through increased productivity.

Packaging is a tool that is used to contain the product, protect the product from environmental hazards and help in marketing the product. Examples of ideal natural packaging systems are that of a banana, orange, coconut and egg, where the packaging is provided by nature. The major step forward in the food processing arena has been the ability to protect and preserve products with packaging and hence ensuring the availability of fresh and processed products out of season and over long distances in various forms. Milk is a highly perishable product and is not uniformly available round the year to the whole country due to geographical and genetic variations. If dairy products are to be available all the year round the country, irrespective of the season and area, it must be presented in packages that are convenient to purchase and use.

Packaging has additional responsibilities of providing convenience, safety and facilitating, besides handling, storage and distribution of products. It should prevent spoilage of food by microbial or insect attack. It also preserves the quality and nutritive value of many foods by the exclusion of oxygen and the control of moisture loss or gain. It increases the shelf-life and ensures longer availability and contributes to the product image through structural and graphic design. Product information is also given out through the medium of labeling, which acts as a tool for marketing and advertising.

Packaging means a coordinated system of preparation of goods for distribution, storage, merchandizing and shipment at optimum cost compatible with the requirements of the product. It also includes the art, science and technique used, preparatory to and during transportations and selling of goods and technical methods and work processes related to the above preparations.

The broad range of dairy products necessitates that packaging equipment and materials encompass nearly all types from flexible to rigid and from high to low barrier. The general types of packaging and equipment are discussed below with a focus on fluid products but it must be recognized that other dairy products such as cheese have their own particular packaging requirements and technologies.

Role of packaging in distribution and sale: The essence of what makes milk and dairy products important source of human nutrition also causes rapid deterioration and high perishability. The major role of dairy packaging is to retard the deterioration by preventing microbial recontamination and excessive chemical deterioration. Fluid products are especially susceptible to microbial, enzymatic and chemical degradation. In addition to retarding quality loss, packaging must provide containment, facilitate use, identify products, communicate, and appeal to consumers, all with maximum production efficiency. The large volumes and low unit value means that these functions must be met with minimal economic cost and environmental impact. Because food packaging is generally highly regulated, legal requirements must also be understood and met.

Packaging is an integral part of fruit and vegetable processing. It has  major influence on storage life and on marketability of fresh as well as processed products. Packaging fulfills several functions including containment, facilitating transportation, protection of fruit/vegetable from further damage, protection of environment from contents of package (for example, if the contents are dirty), marketing, product advertising, and stock control.

Packaging

Packaging is a mean or system by which a fresh or processed product will reach from the production center to consumer in safe and sound condition at an affordable price.

Objectives of packaging and packaging materials

The primary objective or packaging is to protect the contents during storage, transportation and distribution against deterioration. It may be physical, chemical or biological. However, followings are objectives of packaging and packaging materials.

India’s bakery market at 49.5 billion tonnes makes it the third largest market in Asia Pacific, only after Japan and Australia. 72 The bakery industry in India comprises of organized and unorganized sectors. The organized sector consists of large, medium and small-scale manufacturers who produce packaged biscuits and bread. The unorganized sector consists of small bakery units, cottage and household type manufacturing their goods without much packaging and distributing their goods in the surrounding areas. Bread market is estimated to be growing at around 7% p.a. in volume terms, whereas the biscuit market has witnessed a higher growth at around 8–10%. Within the biscuit category, cream and specialty biscuits are growing at a faster rate of 20% p.a. The per-capita consumption of biscuits in India is around 900 gm as compared to 15–20 kg. for developed countries. The consumption of biscuits is equally divided between the urban and rural population. Demand for biscuits in 2003–2004 is likely to exceed 1.2 million tonnes.

Introduction

Packaging and storage are two most important operations in post harvest supply chain of fresh and processed fruits and vegetables. The self-life, damage, loss, sale and profit from commodities depend on storage and packaging parameters. The primary objective of packaging of fruits and vegetables is to protect the contents during storage, transportation and distribution against deterioration, which may be physical, chemical, or biological. Packaging is hence provided at the point of production or processing or at distribution centers. Though packaging forms the last link in the chain of production, storage, marketing and distribution, it still plays an important role in delivering the contents safe from the ‘‘farm gate to the consumer plate.’’ Increase in production can have an impact on the consumer only when the food is wholesome, unadulterated and available under hygienic conditions at an economical price.

In may cases, it becomes necessary to send the cells from one laboratory to another laboratory within or outside the country. The ideal method is to fill the flask containing cells at log/exponential phase with growth medium, tightly close the cap, seal it with parafilm and tape and pack it properly with polythene containing tissue paper and cotton. The tissue paper and cotton is used to soak the medium in case of accidental breakage. The polythene containing tissue culture flask having the adherent cell sheet should again be packed with thick paper. Finally, it is to be kept in a wooden/metal box or container.

Introduction
 
The emerging functional foods market presents a considerable challenge to all manufacturers to keep abreast of the use of adequate packaging materials for their products. The interest in functional foods is intrinsically linked to the potential health benefits of their bioactive components. Preserving these bioactive components is, therefore, a crucial factor to providing the anticipated health benefits of functional foods. However, recurring failure in maintaining the quality of functional foods has been reported. Functional foods include a wide variety of products such as prebiotics and probiotics, dried foods (fibers, probiotics, tea, herbs, etc.), fermented products (yogurts, kefirs, vegetables), and fresh fruits and vegetables.

Introduction

Packaging is an integral and pervasive part of contemporary world. Packaging makes material handling convenient, economical and effortless and modern consumer marketing will seem virtually impossible without packaging. For an engineer or food scientist, packaging is specialized arrangement of matrices fabricated for the delivery of safe food products with maintaining high quality and food safety, at every step of distribution chain including procurement of raw material to manufacturing of finished product; packaging of product; storage; transportation and distribution to wholesaler, retailer and consumer and even for recycling operations [17].

Packaging of food is imperative in a world dominated by marketing-based economy The fact that 73% of purchase decisions are made at point of sale highlighted the importance of a good package design in marketing. During scanning of food package, consumer made quick and rapid perception about the inclusion of a particular food based on the various verbal and nonverbal attributes embodied on food package [25]. Designing is an indispensable are of food packaging. It is medium to attract potential buyers and distinguished the product from the competitors. The presence of visual attributes on package design will not only ameliorates the food product and glamorizes the packets but also defines its authenticity and peculiarity. Therefore, a quality package design is characterized as communication channel between the manufacture and consumer, and unless performed correctly the market image of the brand and product suffers and influence consumer’s perception.

Packaging machinery is a critical component in the food industry, enabling the efficient and consistent packaging of food products while maintaining safety, quality, and freshness. From automated filling and sealing systems to advanced inspection and labeling machines, packaging machinery plays a pivotal role in delivering food products to consumers in optimal condition. The machinery used in food packaging is designed to handle various product types, from solid and liquid foods to powders and granular items, each requiring specific handling, filling, and sealing techniques. In an industry where speed, hygiene, and precision are paramount, packaging machinery has evolved to include sophisticated features such as robotics, computer-controlled systems, and sensors that monitor critical parameters like weight, temperature, and fill levels. These innovations help manufacturers improve productivity, reduce waste, and ensure that food products meet regulatory standards and consumer expectations.

The choice of packaging materials is fundamental to the effectiveness, sustainability, and appeal of food packaging. Different materials offer distinct properties and functionalities, from providing a secure barrier against contaminants to enhancing convenience for consumers. Packaging materials are categorized based on their characteristics, including flexibility, rigidity, and barrier capabilities, each suited for particular types of food products and storage conditions. In recent years, there has also been a shift toward more sustainable materials, such as biodegradable and recyclable options, reflecting growing environmental concerns and regulatory pressures. This chapter explores the various types of packaging materials used in the food industry, examining their benefits, limitations, and applications. By understanding the unique properties of each material, we can better appreciate how they contribute to the protection, preservation, and presentation of food products.

Abstract

Packaging industry is considered to be one of the world’s largest industrial sectors. In the past few years, food processing has undergone several paradigm changes with respect to production, distribution, storage, processing and retailing. This increasing dynamism in the food industry, while on one hand placing a lot of challenges on the packaging industries, is on the other hand opening up opportunities for entrepreneurs to foray into food processing and also packaging industries and develop appropriate solutions in response to the needs of the market and environment. This study aims at exploring the state of packaging industry and packaging technologies in developing countries, with special emphasis on India, against the backdrop of increasing concern for prevention of food losses and therefore highlighting the need for new innovative ideas for food packaging. The authors posit that customer awareness about safety of processed food has given rise to tremendous possibilities of entrepreneurship opportunities in the area of safe and hygiene packaging. However market preferences, state reputations and cost benefit analysis play major roles in packaging of processed food products.

India is the second largest producer of fruits However, the export potential of fruits and vegetables is very negligible. This is because of improper knowledge to the farmer about proper post-harvest infrastructure facilities. Fruits have a high-water content ranging from 70-95 per cent. They dry quickly under any normal atmospheric condition. These causes loss of rigidity and shrinkage of the cells cause wilting and shrivelling. Appropriate packaging ensures prolonged the shelf life of fruits and vegetables by preventing moisture loss and thereby wilting. Transportation is a critical phase in the fruit supply chain that can significantly affect the quality of fruits. Several factors during transportation can lead to quality degradation such as temperature/humidity fluctuations, mechanical damage, transit time etc. To mitigate the adverse effects of transportation on fruit quality, best practices ie, real-time monitoring, temperature and humidity control, loading/unloading techniques can be employed. Quality evaluation is a critical component in the fruit industry, encompassing a range of assessments to ensure fruits meet the desired standards for consumer satisfaction, shelf life, regulatory compliance, and market competitiveness. It involves a comprehensive approach that includes physical, chemical, and sensory assessments, supported by advanced technological tools, to maintain and enhance the quality of fruits throughout the supply chain. This book chapter deals with the effective packaging, transportation, and quality evaluation of fruits, ensuring that consumers receive fresh, high-quality produce.

INTRODUCTION

Packaging is an important aspect in modern life and in fact the word ‘packaging’ developed a literal value indicating presentation of an issue or material in a comprehensive and compact manner. Food packaging has become a revolutionary trend and plays a dominant role in the promotion of marketing of various fresh and processed food products. The reason could be a number of aspects inclusive of protective, legal and aesthetic elements. The industrial revolution in the late 18th century caused major impact on manufacturing process. Without appropriate packaging no food is acceptable and even in the common household food packaging is being practiced in its own way to render food safety and convenience for various culinary operations. Therefore, food packaging needs to be considered as an essential element of food processing and marketing and it is one of the key areas in food technology making rapid strides in research and development.

11.1. Introduction

Food packaging is the integral part of the food industry. Packaging may be defined as the enclosure of products, items or packages in a wrapped pouch, bag, box, cup, tray, can, tube, bottle or other container form to perform one or more of the following functions: containment, protection, preservation, communication, utility and performance. If the device or container performs one or more of these functions, it is considered a package. The major roles of the packaging of food are the containment and preservation of foods by protecting the food from physical, chemical and microbial spoilage. Besides, packaging performs some other functions like communication which means giving the information about the product, transportation of the food production and so on. Food products are classified as perishable, semi- perishable and non-perishable food based on the spoilage. Selecting appropriate packaging material and packaging technique for various food products like meat and poultry, fruits and vegetables, milk and milk products, bakery and confectionery and beverages is essential to maintain the quality of food product. Selection of the ideal packaging material for specific food requires prior knowledge about the packaging materials and its properties, type of the food products and specific spoilage mechanism of the particular food. Above all, from economical point of view, packaging material cost and capital for the establishment of the packaging technique also plays crucial role in selection of a particular packaging material or technique. 

Packaging : Packaging of fruits, vegetables, flowers or any other commodity is chiefly done to assemble the produce in convenient units of different sizes, shapes & types for storage, transportation, marketing and distribution. The size of units may vary depending on the requirement e.g for retail marketing smaller units are preferred and for bulk transportation and storage, large units would be more convenient.

Why do we Require Packaging?

Imagine there is no packaging material and technology available. How we are going to transport apple, mango etc. to distant markets? Definitely as loose. Then how can we weigh or count the fruits being transported or marketed? This would be very difficult. Had there been no packaging the commodity would have suffered great physical, chemical and microbiological losses. It would have been very difficult to handle, manage, transport and market the produce as loose and unpacked. Therefore, the packaging of fresh as well as processed commodities was needed to contain, unitize and protect the produce.