eChapters

Fracture healing is a specialized type of a wound healing response in which the regeneration of bone leads to a restoration of skeletal integrity. Because healing of a fracture in a living organism is accomplished by cells, it can be modified by almost any endogenous or exogenous factors like nutrients, vitamins, hormones, growth factor etc. that has an influence on the metabolic function of bone cells. Fracture healing may be induced by malnutrition that involves certain nutrients, notably calcium (Ca), phosphorus (P) and vitamin D, but also iodine (I), copper (Cu), zinc (Zn), manganese (Mn), fluorine (F), silicon, vitamin A and perhaps vitamin C as well as different endocrines. The number of these nutrients and their interaction is so large that dietary intervention in fracture management is likely to be the most effective if directed at consumption of a good diet that is completely balanced for growth, maturity, old age or stress. Substitution of a good diet for a bad one is also likely to be more convenient and safer than most attempts at partial supplementation of a faulty diet in order to bring it into balance. Underfeeding a good diet decreases size-for-age of bones during growth and delayed fracture healing. Underfeeding may engender osteoporosis in older animals. Underfeeding of Ca, P and vitamin D, causes delayed fracture healing. Over feeding a balanced diet induces rapid growth of newly formed bone and sometimes-accelerated fracture healing but it also causes obesity in adults.

Three major component of bone are osteogenic cells, organic matrix and inorganic matrix (mineral). The osteogenic cells include osteoblasts, osteocytes and osteoclasts, while the matrix consists predominantly of collagen and proteoglycans and constitutes approximately one-third of bone mass (on dry basis). The inorganic/mineral component that makes up approximately two-third of bone and it performs two essential functions : it serves as an ion reservoir and it gives bone most of its stiffness and strength. Bone is a reservoir for 99% of body calcium, 85% of body phosphorus and 40-60% of body sodium and magnesium. The main mineral composition of a long bone is: 85% calcium phosphate as hydroxyapatite, 12% calcium carbonate and 3% magnesium phosphate, calcium fluoride and magnesium carbonate and trace amount of chloride, potassium and sodium. For collagen synthesis, hydroxylase enzymes requires ferrous ion and vitamin C. Impaired collagen synthesis also occurs during deficiencies of vitamin D and E and zinc. The organic intercellular bone matrix is rich in mucoplysaccharide. Their synthesis involves enzymes that require manganese, zinc and vitamin A.

Bone differs from other tissues not only in physio-chemical structure, but also in its extraordinary capacity for growth, continuous internal remodelling and regeneration throughout post fetal life. Bone formation is a complex process regulated by diet, vitamins, hormones and growth factors. Growth factors are polypeptides that increase cell replication and have important effects on differentiated cell function. Growth factors are initially considered as systemic agents, but current evidence indicates that they act primarily as local regulators of cell growth. The transforming growth factor-β family of proteins, which comprises polypeptide growth factors that have diverse effects on the growth, differentiation, and function of cells, is receiving considerable attention for potential clinical applications and is likely of physiological and orthopedically significance.

Transforming growth factors

It is one of the most important growth-promoting osteo-inductive substances, which have been identified at the site of fractures and other places. Transforming growth factors (TGFs) have been categorized into TGF-β (alpha) and TGF-β (beta). TGF-α has not been isolated from bone tissue and cannot be considered a local regulator of bone remodelling, although it is mitogenic for bone cells and stimulates bone resorption. TGF-α shared amino acid sequence, receptor-binding and functional activity with epidermal growth factors but had only weak transforming growth factors activity. Furthermore, TGF-β by itself is completely ineffective, but together with TGF-α, it potently induced growth of colonies of cell-line.

The knowledge and principles from interconnected disciplines of bioengineering, material science and life sciences are combined in tissue engineering with the aim of developing a construct, that can wholly or partly restore/maintain/augment the function of a damaged tissue or organ. Tissue engineering construct generally comprises cells on a suitable scaffold. The ultimate functionality of a tissue engineering construct is mainly dependent on the cell behavior on scaffolds. The proliferation and cell viability on scaffolds varies from one cell type to another and from one species to another. So it is important to investigate individual cell-scaffold combinations. The similarity in structure and composition to bone mineral, osteoconducive properties, ability to integrate with the bony tissue and absence of immune response makes hydroxyapatite [Ca₁₀ (PO₄)₆(OH)₂] the choice of calcium phosphate biomaterial with only disadvantage of slow rate of resorption. On contrary, silica–calcium phosphate composite in comparison to calcium phosphate is rich biomaterials having a faster resorption rate. Studies have shown that coating of hydroxyapatite with a calcium silicate containing layer encourages cell proliferation and osteogenic differentiation of human bone marrow-derived stromal cells. So in this chapter, a triphasic composite scaffold (calcium silicate, hydroxyapatite and tricalcium phosphate), namely HASi with elements in the following percentages: 66.36 % - calcium, 25.35 % - phosphorus and silicon - 8.29 % and porosity of 50 – 500 µm is reported to evaluate the rBMSC proliferation and differentiation properties in vitro.

The present chapter is therefore, included with the aim of assessing cytocompatibility of HASi and rBMSC in terms of cell attachment, cell morphology, cell proliferation and osteogenic differentiation.

Fracture healing is a specialized type of wound healing response in which the regeneration of bone leads to restoration of skeletal integrity. Though the process of fracture healing is usually considered to be biologically optimum; the healing of 5-10% is delayed or impaired. For management of these types of fractures, use of biological methods for the enhancement of fracture healing is essential. Furthermore, fracture-healing can be modified by almost any endogenous or exogenous factor that has an influence on the metabolic function of cells. These factors include transforming growth factor-beta, bone morphogenetic proteins, hormones, vitamins, prostaglandin and calcium containing feed supplement medicine.

Keeping in view the above, in this chapter, efficacy of herbal product-Magacal in promoting osteogenesis during fracture healing in experimental animals is discussed. Magacal optimizes absorption, bioavailability and utilization of dietary calcium, phosphorus and magnesium. It is claimed to have advanced chelating agent to form organic complex chelate with dietary Ca, P and Mg, besides containing natural Vitamin D metabolites in highly bioavailability organic form, naturally protected and stable biotin and modern delivery system for optimum translocation of Ca, P and Mg ions to the sites of requirements.

Clinically healthy, adult New Zealand White rabbits of either sex (10) are divided equally into two groups A and B each consisting of 5 animals. All animals are kept on standard diet and management throughout the experiment. In all animals, midshaft simple transverse fracture is created in both the ulnae under thiopental (2.5%) anaesthesia. In test group (Group A), Magacal is given @5ml/animal/ day, orally from day 5-20 post fracture, whereas, in group B, no drug is given which serve as control. Antibiotic dressing of the skin wound is done daily for one week with povidone iodine and himax ointment. All the animals are administered streptopenicillin @ 0.5 gm/animal/day for 5 days postoperatively. Skin sutures are removed on the10th postoperative day.

Clinically all the animals are observed for inflammation, pain and edema at the fracture site, surgical wound healing, appetite, general health, muscular atrophy of the limb, if any, at different intervals postoperatively.

Minimally invasive percutaneous osteosynthesis (MIPO), or minimally invasive fracture repair, was introduced into human orthopedic surgery during the 1990s because of several distinct advantages over traditional open reduction techniques. In MIPO, large incisions to facilitate open reduction are avoided and implants are introduced through small stab incisions in the skin. This led to advent to more biology friendly techniques. These biological techniques lay stress upon maximal preservation of blood supply around the fractured bone by minimal direct handling of the fracture environment. MIPO offer advantages which include preservation of the fracture hematoma; less surgical trauma to the surrounding soft tissues, reduced operative time therefore, decreased risk of infection and ultimately leading to indirect bone healing with abundant callus. Minimally invasive plate osteosynthesis (MIPO) is a novel technique for application of principles of biological fracture healing with a philosophy of dealing with soft tissues with utmost respect. Following advancements in implant technology and development of techniques of indirect fracture reduction this technique gained more popularity. The evolution of MIPO began with the use of bridge plating. Initially a conventional plate is applied for comminuted femoral fractures using long incisions but with preservation of the vastus lateralis muscle resulting in healing with abundant callus. Subsequently, the size of incisions got decreased, rather two small incisions are given at proximal and distal ends and plates are bridged sub muscularly over the fracture fragments. This technique causes minimal distress to soft tissues and bone, provides access to the bone through soft tissue windows, with minimal or no contact with the fracture by indirect reduction tools and leaving behind minimal foot prints.

One of the most common fractures seen in the dog is comminutuion of femoral shaft, often related to sever impact from an automobile accident or jump from a height. Comminuted fractures are characterized by one or more butterfly fragments and assorted small bone chips that are especially difficult to align accurately and stabilize completely. These complicated fractures require sophisticated repair technique that are capable to resisting compression, bending and rotational forces. Rebuilding of comminuted fractures so that all fragments are accurately aligned and gaps are eliminated is often impossible. Non-union and osteomylitis have their highest incidence in these fractures. Over the years, several techniques are tried to treat comminuted femoral fractures with different success rate and there is a lack of uniform fracture presentation. Hence, it will be difficult to compare different techniques in a particular type of fracture. The present chapter is, therefore, undertaken to evaluate radiographically and histopathologically the efficacy of three different internal fixation techniques in the management of comminuted diaphyseal femoral fracture in dogs.

Clinically healthy, adult mongrel dogs (15) of either sex are randomly divided into groups A, B and C of 5 animals each. In all the animals unilateral comminuted diaphyseal femoral fractures is created under general (thiopental - 5%) anaesthesia and is immobilized with intramedullary (IM) pinning with cerclage wiring (Gr. A), neutralization bone plating (Gr. B) and neutralization bone plating with cerclage wiring (Gr. C) respectively. All the animals are administered broad spectrum antibiotic (streptopenicillin @ I g intramuscularly (dog/day) and analgesic (diclophenac sodium @ l mg/ kg b. wt. daily) and surgical wounds are dressed daily with povidone iodine and antibiotic cream for 5 days postoperatively.

Lateral and oblique views of radiography are taken at the operated site just after the operation and subsequently on days 15, 30, 45 and 60 postoperatively. The radiographs are observed for status of fracture reduction and fixation, progress of healing at the fracture site and complications like fixation failure, osteomylitis etc., if any, at different intervals. Bone sections are collected from the fracture site including adjacent normal bone after sacrificing the animals on day 60. The sections are decalcified using Goodling and Stewart’s solution (80% distilled water, 15% formic acid and 5% formalin) and then processed routinely for H & E staining for histopathological studies

Healing of fracture is a consequence of cellular behaviour in a living organism and as such can be modified by almost all endogenous or exogenous factors that affect the metabolic function of cells. These factors include transforming growth factor-β, bone morphogenetic proteins, hormones, vitamins and prostaglandins. The anabolic hormones like thyroxine and insulin accelerate bone formation in experimental animal. Hence, this chapter is proposed to evaluate the effects of thyroxine and insulin therapy in the management of tibial metaphyseal fracture in dogs.

Clinically healthy, adult mongrel dogs (15) of either sex are randomly divided into three groups (A₁, A₂ and A₃) of 5 animals each. In all the animals, unilateral metaphyseal fracture is created in the proximal tibia and then immobilized with cross intramedullary pinning with interfragmentary wiring under thiopental (5%) anaesthesia. All the animals are administered broad-spectrum antibiotic (streptopenicillin @ 1 g intramuscularly /dog/day) and analgesic (5 ml I/M daily) and surgical wounds are dressed daily with Povidone iodine and Lorexane cream for 5 days postoperatively. In group A₂, thyroxine sodium tablets (0.013 mg/kg body wt, orally) and in A₃, insulin injection (2 IU/kg body wt, s/c) are given on day 7 and then on every alternate day till day 45 postoperatively, at one specific time. Group A₁, is remained as control, in this group no hormonal therapy is given.

Craniocaudal and mediolateral radiographs of the fracture site are taken immediately after surgery and subsequent on days 15, 30, 45 and 60 postoperatively. The radiographs are observed for status of fracture reduction and fixation immediately after surgery and subsequently for the maintenance of reduction, progress of fracture healing and complication, if any, at different intervals.

SOIL WATER

Soil water and oxygen share the pore space in soil and generally the oxygen content is determined by the water content, which is added by irrigation or rains and then keeps on changing due to evapotranspiration. Water is the vehicle by which most nutrients move from the soil to root surface. But for the lowland rice, nutrients are most available to plants at ?eld capacity moisture (about 0.3 atmosphere soil water tension) and the availability decreases as the moisture content decreases to wilting point (about 15 atmosphere tension). That is why rainwater or irrigation increases nutrient availability, while extremely dry condition decreases it. There is thus a signi?cant interaction between water and nutrient availability in soil (Singh et al., 2014). In a study in India (Singh et al., 1980) the yield of wheat was signi?cantly affected by irrigation water and nitrogen treatments; maximum yield was obtained with irrigation at 50% available soil water in 60 cm soil depth and 120 kg ha ^–1 nitrogen addition. A study on wheat at Delhi (Kumar et al., 2015) very well shows the effect of irrigation water on yield and uptake of nutrients (NPK) (Table 5.1).

There are nine essential micronutrients, namely, iron (Fe), manganese (Mn), zinc (Zn), copper (Cu), boron (B), molybdenum (Mo), chlorine (Cl), vanadium (V) and nickel (Ni). Out of these, Fe, Mn, Zn, Cu and Ni (Dominic et al., 1978) are taken up by plants in their ionic form X²⁺, Mo as MoO^2-, chlorine as Cl^– form and boron as boric acid (H₃BO₃) or as its ionic form B (OH)₄ ^–. The absorption of vanadium by plants has received only limited attention (Lepp et al., 1983); it may be absorbed as cation V or vanadyl (VO²⁺) and anion vanadate (VO₄ ^3–). Bulk of the information is available on Fe, Mn, Zn and Cu, because of the acceptability of the single DTPA (Diethylene triamine pentaacetic acid)–CaCl₂ extraction test of Lindsay and Norvell (1978) for availability of all these four nutrients followed by their estimation on Atomic Absorption Spectrometer (AAS). Available B is estimated by hot 0.15 M CaCl₂ extraction method (Saha and Singh, 1997). Molybdenum data base is poor, because its determination is dif?cult and Mo de?ciency in crops has not been much noticed in India. Chloride is available in plenty in irrigation water and soil in India and has not received much attention in India.

Internationally maize (Zea mays) and citrus (Citrus sp.) are reported to largely suffer from Zn de?ciency, for which these are considered as marker plants. Zinc de?ciency in maize results in pale or whitish top young leaves and the de?ciency is referred to as 'white bud' (Camberato and Maloney, 2012). In citrus, Zn de?ciency results in ‘rosette’ (cluster of stiff leaves at the end of young shoots) formation (Brennan, 1993). Zinc de?ciency in India was ?rst reported in rice by Nene (1966) at the Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, and given the name ‘khaira’ disease due to dark-brown-coloured spots resembling kattha (Acacia catechu extract used in paan – the betel leaves) on the leaves of zinc de?cient plants. Soon All India Coordinated Project on Micronutrients was launched by the Indian Council of Agricultural Research (ICAR) with headquarters at the Punjab Agricultural University, Ludhiana, which was later shifted to the Indian Institute of Soil Science, Bhopal, Madhya Pradesh. About half of the cultivated soils in the India are de?cient in available zinc. Zinc de?ciency is most in Maharashtra and Karnataka and rice-growing states of north and northeast (Table 12.1). A good response of cereals to Zn fertilization has been reported (Rattan et al., 2008).

Soil-rejuvenation power of legumes in crop rotations has been known in several parts of the world since ages. It is mentioned in the Indian agriculture of the Chalcolithic period (ca 1000 BC) (Raychaudhuri and Mira, 1993), in Chinese agriculture in the Han dynasty (ca 2nd century) (Hsu, 1980) and in Roman agriculture (White, 1970). Legumes can be ?tted in cropping systems in India in a number of ways, such as, a crop in a cropping system, a catch crop in an intensive cropping system, an intercrop and as a green manure. A brief discussion on these follows.

The introduction of high-yielding varieties of wheat and rice, the development of intensive cereal–cereal rotations (rice–wheat, rice–rice, hybrid maize–wheat), application of high doses of N (above 100 kg N ha^–1 to each crop) without adequate P and K application, increased use of high-analysis fertilizer (urea and diammonium phosphate in place of ammonium sulphate and superphosphate) and ignoring the application of organic manures led to heavy depletion of P, K, S, Zn and other plant nutrients, which led to wide-spread de?ciencies of P, S and Zn in Indian soils. These de?ciencies of nutrients led to focus on integrated nutrient management.

Plant nutrients are important for increased production of food for the ever-increasing world population. Of the plant nutrients, primary nutrients N, P and K are applied to ?elds each season in fairly large amounts. In addition to increasing crop yields, they also impact the environment. This is briefly discussed in this chapter; only main points are made here.

NITROGEN

Nitrogen is blamed the most for environmental degradation. It contributes to eutrophication of inland and sea-waters, enrichment of air with NH₃ and N₂O and even with global warming. However, it is almost impossible to grow more food without additional nitrogen. It is estimated that by 2050 about 250 million tonnes (MT) of fertilizer nitrogen may be needed to meet global increased food demands as against an estimated current consumption of 116 MT

Cohn (1875) first observed and described a branching filamentous microorganism in the ‘concretions’ (coagulated mass) taken from a lacrimal canal, later identified as Actinomyces israelii. Bollinger first reported the yellow granules in jaw tissues of the cattle in 1877 and established the significance of the bacteria as the etiological agent. In 1878, Israel described the first human case. In 1879, Hartz observed the microscopic appearance of granules of Actinomyces and suggested the name Actinomyces bovis. Mosselman & Lihnaux (1890) and Wolf & Israel (1891) first isolated the bacteria in artificial laboratory media from lumpy jaw cases of cattle and human actinomycosis, respectively.

Streptomyces are soil-borne bacteria known for production of several antibiotics, antifungals, antivirals, antitumoral, anti-hypertensives and immunosuppressives. Krainsky (1914) first described S. griseus and named the species Actinomyces griseus. Waksman and Henrici (1948) changed the name to Streptomyces griseus.

Dermatophilus congolensis was first described from clinical case of exudative dermatitis in cattle in Congo by Van Saceghem (1915). It is primarily known as an animal pathogen causing acute, sub acute or chronic skin disease. The first cases of human infection with D. congolensis were reported in 1961 in four people who developed skin infection after handling an infected deer in USA.

Properties

1. Bacteroides, Dichelobacter and Fusobacterium are major gram negative anaerobes isolated from human and animals. Other gram negative anaerobes are listed below (Table-1).

2. The bacteria are tolerant to oxygen in comparison to strict anaerobes. The bacteria do not die after oxygen exposure.

3. The bacteria naturally inhabit the intestine and mucosal surfaces of human and animals. Majority of them are opportunistic pathogens. Often they cause mixed bacterial infection along with aerobes. The aerobes remove oxygen and create an anaerobic condition favourable for their growth. The aerobes may also supply other growth requirements. For e.g. Porphyromonas asaccharolytica requires vitamin K for its growth, supplied by another aerobic bacterium, not the host.

The word ‘chlamydia’ comes from the Greek word ‘chlamys’ means ‘cloak draped around the shoulder’. It describes how the intracytoplasmic inclusions produced by the bacterium appear under the microscope(‘draped’ around the host cell nucleus). Chlamydia was first discovered by a Czech zoologist and parasitologist, Stanislaus von Prowazek in 1907 in Berlin. Originally because of its structure it was thought to be a virus. In 1963 it was recognized as a bacterium rather than a virus.

Properties of genus

1. Morphology: Chlamydia are Gram-negative, non-motile, pleomorphic and obligate intracellular bacteria. The Gram-negative cell wall consists of cytoplasmic and outer membrane. However, they lack peptidoglycan layer. Moreover, they also encode major outer membrane protein (MOMP), the key antigenic determinant used for serological differentiation of chlamydiae.

They are poorly stained by Gram’s stain. Chlamydiae are readily stained by Castaneda (blue in appearance), Giemnez and Macchiavello (red in appearance) methods. However, Giemsa staining is preferable to stain the inclusions (chlamydial micro colony) in the cell culture. Inclusion bodies of the Chlamydiae are basophilic in nature. The staining of the inclusion bodies may differ depending on the species. In C. trachomatis, the inclusion bodies contain glycogen matrix and can be easily stained with iodine solution.

World Scenario

The world climatic conditions are divided into Tropical; Sub-Tropical and Temperate climates. Fruits and Vegetables suited to these climatic conditions are grown successfully. Considering the nutritional and economic importance of fruits and vegetables, there is tremendous increase in area and production of fruits and vegetables throughout the world. The present global scenario of fruit and vegetable crops (areas harvested, production and productivity (yield) has been given in Table 1. It shows that the fruits 865.88 million tones has been harvested from 65.24 million Ha land with productivity 13.27 t/ha (Table 1) where as vegetables produced 1075.20 million tonnes from area 57.00 million ha with yield 18.86 tonnes/ha (Table 1) during 2016.

Fermentation

The decomposition of carbohydrates by microorganisms and enzymes is known as fermentation. During fermentation process of food organic acids and alcohol etc are produced which act as preservatives and also provide fine flavor and aroma that increase the value of raw materials. Most fermented foods contain a complex mixture of carbohydrates, proteins and fats etc. Fermentation occurs when microorganisms consume susceptible organic substances as a part of their own metabolic processes. Natural fermentation is probably oldest form of food preservation.

History in general reveals chronological account of important events, contribution of persons who significantly influenced the thinking of their era and the interpretations of the observed facts or phenomenon over the period of time. The progress in plant pathology leading to the major land mark in mycology, plant bacteriology and plant virology will be discussed in chronological order for easy and better understanding.

a) Definition

Fungus is Latin word meaning ‘mushroom’

Alexopoulus and Mims 1979 defined fungus as eukaryotic, achlorophyllous organism generally produced by sexual or asexual method and whose filamentous branched somatic structure is typically covered by cell wall.

Bacteria

Definition

Bacteria belong to prokaryota which encompasses organisms with a primitive type of nucleus lacking a clearly defined membrane. The bacteria are smaller than fungi and measure about 0.5 to 1.0 x 2.0 to 5.0µ.

Definition

Neamtode may be defined as triploblastic, bilaterally symmetrical, unsegmented, pseudocoelomate individual invertebrate, with 4 main hypodermal chords, a triradiate oesophagus, circum oesophageal nerve ring, and lacking specialized organs for respiration and circulation.They have tubular gonads which open separately in females but join alimentary canal in males to open through a cloacal aperture.

Pathogenesis: Role of enzymes

The chain of metabolic events that bring about the disease is pathogenesis. It involves the virulence of the pathogen, susceptibility of the plant and impact of the environment. It is called disease triangle. Penetration infection, invasion, colonization and reproduction are the events that occur during pathogenesis, and lead to development of disease.

The establishment of pathogen in the host and consequent development of disease involves a chemical interaction between the host and the pathogen. The pathogen employs offensive chemical weapons to break host barriers, which the host resists with all its might. Parasitism and disease resistance run parallel and are inseparable, A biochemical ‘tug of war’ ensures between the host and the pathogen, in which one tries to outwit the other. Enzyme, toxins and growth regulators are the important chemical weapons of the pathogen. Viruses, however, because of their basic nature, do not produce any of these chemicals.

Pathogens produce these substances either in normal course of their activities (constitutively) or when, they grow on certain substrates such as their host plants (Inducible). Plant pathogenic enzymes disintegrate the structural components of host cells, breakdown inert food materials in the cell, or affect components of the protoplast directly and interfare with its functioning system. Toxins seem to act directly on protoplast component and interfare with the permeability of its membrane and with its function. Growth regulators exert a hormonal effect on the cells and either increase or decrease their ability to divide and enlarge. Polysaccharides seem to play a role only in the vascular diseases, in which they passively interfere with the translocation of water in the plants or they may also be toxic.

Disease Forecasting

Definition

Forecasting involves all the activities in ascertaining and notifying the grower of community that conditions are sufficiently favourable for certain diseases, that application of control measures will result in economic gain or on the other hand, and just as important that the amount expected is unlikely to be enough to justify the expenditure of time, energy and money for control. The above statement made explicit distinction between positive forecast and negative forecast and both have value for growers as well as society in general.

Positive forecast: Employs need based chemical sprays, provides adequate protection to crop and reduces damage to environment.

Negative forecast: Avoids unnecessary chemical sprays, no risk to the crop health and no disruption of environment.

1. Biosecurity

Biosecurity is strategic and integrated approach encompasses policy frameworks to analyze and manage risks for food safety, animal life and health and plant life and health including environment.

Definition

Garret (1970) defined biological control as any condition under which or practice whereby survival or activity of a pathogen is reduced through the agency of any other living organism (except man) with the result that there is a reduction in the incidence of the disease caused by that pathogen.

Baker and Cook (1974) stated that the biological control is the reduction of inoculum density or disease producing activities of a pathogen or parasite in its active or dormant stage, by one or more organisms, accomplished naturally or through manipulation of the environment, host or antagonists, or by mass introduction of one or more antagonists.

Biocontrol can be simply defined as the application of living organism to control diseases.

Definition

Those characters that enable a plant to avoid tolerate or recover from attacks of pathogens under conditions that would cause greater injury to other plants of the same species.

Green revolution technologies such as greater use of synthetic agrochemicals like fertilizers and pesticides, adoption of nutrient-responsive, high-yielding varieties of crops, greater exploitation of irrigation potentials etc. has boosted the production output in most cases. However, continuous use of these high energy inputs indiscriminately now leads to decline in production and productivity of various crops as well as deterioration of soil health and environments.

Cropping system is defined as the cropping pattern followed on a farm and its interactions with farm resources, other farm enterprises and production technology. The yearly sequence and spatial arrangement of crops and fallow on a given area is termed as cropping pattern.

India has over 17% of world’s population living on 2.4% of the world’s geographical area. Per capita agricultural land has reduced by 67% from 0.48 ha in 1951 to 0.16 ha in 2008 due to explosive increase in population. In this situation, it is very difficult to get horizontal expansion in vegetable cultivation. Hence, total production per unit area can be increased by increasing the productivity per unit area through (a) growing improved high yielding varieties and hybrids, (b) adequate and scientific crop management practices and (c) increasing cropping intensities following different cropping patterns.

Human beings, considered to be the highly evolved living organisms, have been making consistent and constant efforts to lead comfortable life without much concern for the conservation of nature and adversities to other living and nonliving entities existing in the universe. To meet the requirements of food, feed, fibre, timber and other needs, the plants that could supply the above were selected and domesticated. When the useful plant species were cultivated over large areas repeatedly year after year, the problems afflicting plants that were not recognized earlier as constraints for crop cultivation, assumed monstrous proportions wiping out entire crops and ruining the livelihood of the people of that geographical location/country. World history is replete with numerous instances of huge crop losses caused by microbial plant pathogens resulting in malnutrition, hunger and death. Migration of millions of people to other countries for their survival, became unavoidable. Such historical evidences threatening the existence of humans necessitated to investigate the causes of destructive diseases like late blight and heliminthosporiose diseases affecting respectively potato and rice, the staple food of the largest populations of the world. In order to take appropriate measures for minimizing losses due to crop diseases and to mitigate the sufferings of man and the animals, application of various short- and long-term disease management strategies has become indispensable for the profitable cultivation of crops

Management of crop diseases by various methods of exclusion in order to reduce the pathogen population in a geographical location / country may be achieved by either preventing introduction of pathogens or by growing disease-free seeds and planting materials. In addition, the inoculum of a pathogen reaching newly planted crops may be significantly reduced by adopting appropriate cultural practices also. The contributions of these factors for successful cultivation of crops are discussed hereunder.

8.1 Prevention of Introduction of Microbial Pathogens

Prevention of pathogen introduction is the primary responsibility of plant quarantines. Quarantines have been established by several countries by enacting appropriate laws that regulate the importation of plants, seeds and asexually propagated plant materials. These materials are placed in post-entry quarantines (PEQs) and examined by technical personnel for the presence of pathogens and pests. It is obligatory for the exporting countries to comply with the regulations of the importing countries. As the movement of plant products has increased enormously, following the adoption of the General Agreement on Tariffs and Trade (GATT), vigilant enforcement of sanitary and phytosanitary measures has become essential. The Food and Agriculture Organization (FAO) appointed an Expert Consultation on the Harmonization of Plant Quarantine Principles to formulate basic principles to determine standards for plant quarantine principles in relation to international trade (FAO 1991). According to the recommendations of the Expert Consultation, the sovereignty of the importing country is recognized and it has the right to implement the phytosanitary regulations deemed fit by that country. However, cooperation among countries was emphasized for the successful implementation of the recommendations by the member countries belonging to one of the eight regional plant protection organization set up after the International Plant Protection Convention (IPPC) held in 1951. A plant pathogen is recognized as of quarantine significance (QS), if its exclusion is considered to be essential to protect agriculture and natural vegetation of the importing country which has to be provide necessary infrastructure and trained personnel to prevent or delay its entry along possible routes. Lists of quarantine pathogens and the regulations to be followed are prepared by the importing country and made available to the exporting countries.

Diseases are caused by different kinds of microorganisms which may primarily attack specific plant organs initially. They may be either confined to the organs in which infection is successfully initiated and may spread to other organs to cause secondary infection. The plant organs initially affected may not show characteristic symptoms in certain cases as in root rot and wilt diseases. The root infection is recognized much later, when yellowing (chlorosis) of the leaves is observed. It may become very difficult to save the infected plants at this stage, since the pathogen is well established in the deeper tissues of the infected plants. However, the spread of the disease to the adjacent plants may be prevented by protective treatments. Integration of various principles of management of crop diseases has to be followed for successful crop cultivation. The combination of practices that can be applied varies with different types of diseases and the crops affected. Hence, the integrated management of crop diseases is discussed for different groups of diseases affecting primarily different plant organs/ tissues. Fungal and bacterial pathogens remaining in the soil or plant debris left over after harvest of previous crops infect the root system initially and crown or the basal portions of the stem may also be affected later.

Plants have to transport water and nutrients absorbed by the roots from the soil and photosynthetic products from the leaves to other plant parts and storage tissues through the stem tissues. Infection by fungal and bacterial pathogens interferes with the movement of the essential materials leading to the derangement of several physiological functions to varying degrees and even collapse of the aerial plant parts.

16.1 Fungal Pathogens causing Diseases Affecting Stems of Plants

Fungal pathogens cause blight, stem rot, rust, anthracnose, die-back and canker symptoms on the stem tissues of several crops. These pathogens may be soilborne or airborne generally, while some of them may spread through water also.

INTRODUCTION

During the last three decades, libraries have shown a considerable degree of momentum in the collection building and readership programme activities involving considerable expenditure of funds. This rapid development of libraries as a consequence, generated several evaluative studies on the usefulness of libraries to society. Until recently evaluations were subjective, based purely on opinions expressed by an individual or group. However, developments in the field of mathematics, statistics, operations research, management science, economics and system analysis have led to a minimization of this subjectivity. In particular, the systems analysis approach has led to the identification and application of appropriate quantitative measuring techniques for evaluating the effectiveness and efficiency of the information services offered by the librarians. This has generated a new field in library and information science viz; ‘Bibliometrics’.

Bibliometric analysis which has now become a well established part of information research and quantitative approach to the description of documents and examination of services is gaining ground both in research and practice. Librarians are facing the problems arising out of ever increasing cost of different types of reading materials and decreasing budgets of libraries.

Bibliometric studies are helpful in solving the problems of librarians. Pritichard¹ defines Bibliometrics as “application of mathematical and statistical methods to books and other media of communication”.

INTRODUCTION

Research and teaching increasingly rely on global networks for the creation, storage, evaluate, and disseminate the knowledge. The 21^st century has brought enormous change in higher education throughout the world as a result of new information and technological developments. We are outfitting our schools, libraries, and homes with electronic technologies, but are we preparing our students and teachers for the onslaught of information that is provided by these technologies? What happens when the student can get more information from the Internet than previously conveyed by a teacher or a textbook? What should a student do when faced with so many informational possibilities ? Which of the information is credible and which is not?

The teacher’s role must evolve from the giver of knowledge into being more of a coach or guide. Teachers, librarians, administrators, and the community must collaborate to develop ways to involve the student not only in using classroom materials but also in using resources from the broader community and the mass media.

Teachers must be prepared to teach student to become critical thinkers, intellectually curious observers, creators, and users of information. In this context university libraries need to be dynamic, take a leading role in assessing the information literacy and computer literacy competence of the teachers and plan for conducting such programmes to improve the situation. One such attempt has been made by the Authors and the result is reported here.

INTRODUCTION

Andries Van Dam of Brown University, USA coined the term electronic book. E-books are print versions of books, which exist in electronic formats and can be read on a machine. The development of e-book started around 1960s by Alan Kay, called the Dyna Book. Stephen King’s ‘Riding the Bullet’ was one of the most popular electronic books available on the web. The electronic documents are easy to access, easy to search, inexpensive and economy in storage. An e-book can be purchased/borrowed, downloaded, and used immediately, whereas to buy a printed book, one must go to a bookshop, or library etc. It can reduce photocopying activities. The concept of Digital library was created based on the electronic documents.

The transfer of ownership of an e-book author is very difficult, whereas such transfer is possible in printed books. It is cheaper and easier for authors to self-publish e-books when compared to printed form. In electronic format there is no hold on licensing. The problems with e-documents are lack of long term access, dependence on the computer, lack of standardization and less permanent. Digital Right Management system is one of the most important development of electronic publishing. It has control over intellectual property. The e-publishers are more concerned about rights. They are offering limited usage for printing, downloading and copying.

INTRODUCTION

The Digital Library concept has evolved to provide solutions for storage, access, dissemination, content delivery and it paved way to mobile library, as we can browse and share content while on the move. Information and Communication Technology (ICT) has given a new shape to the learning process, which includes reading, understanding and gaining information, which becomes knowledge. As the type of format of information changed from written to e-format, libraries assumed the role of digital libraries.

The library is a place which holds the information resources in print and digital formats. The concept of digital library is a major success as it came out of closed four walls of library and reached readers in their home, workplace and even while traveling, with the help of mobile electronic gadgets like laptop, palmtop, e-Book reader etc. e-Learning technology is a wonderful gift provided by the present day technological advancement and innovations. e-Learning is the convergence of learning and the Internet’ and it has brought about profound changes around the globe in the way people learn and train, allowing them to do it anywhere, any time. Through the World Wide Web the users can access e-content from any point, off or in campus, through a computer and connectivity.

INTRODUCTION

Libraries are undergoing rapid changes due to the developments in Information Communication Technology (ICT). Paper based resources are giving way to electronic resources. The dynamic linking capability of web has provided users unrestricted access to information. ICT has brought massive changes in distance mode of education. ICT has helped to introduce many virtual universities in the world, which are operated through Internet and other telecommunication technologies. The e-learning library should organize and present the e-learning resources and services in a way that help users to locate and retrieve materials needed efficiently.

At present e-portals of various organizations come across as gateway for the websites that aggregates information from variety of sources, providing access to information network and set of services through the web. For examples the libraries of National Research Centers of India, Indian Institutes of Technology (IIT), Indian Institute of Science (IISc) and Indian Institute of Management (IIM). The approach of learning of these institutes doesn’t mean composed, organized and packaged e-information resources in a blogging pattern but it is comprehensive and fully accessible to the user as reader and writer. Undoubtedly, a study of e-library environment of these institutes immensely helps us in evaluate the potential of proliferation of digitalization of libraries in India.

Calcareous soils

In arid regions, calcareous soils are developed from limestone or marl parent materials. The content of calcium and magnesium carbonate may be as high as 60 to 70 per cent of the total soil mass. In calcareous soil lime content may vary from a small amount in some places in the profile to appreciable amounts throughout the profile.

Calcium carbonate coatings occur in sand, silt and clay size fractions of calcareous soils. In sub soil, large amounts of lime forms hardpan under high water tables.

In general, pH of the soil increases to alkaline pH with increasing the proportion of water to soil ratio. This may happen in part from a reduction of CO₂ pressure. The dilution of salts in soil solution also permits greater hydrolysis of calcium clay. The dilution of calcium carbonate with production of hydroxyl ions may lead to higher pH as:

Iron Toxic Soils

Lowland rice is being cultivated on approximately 128 million hectares of either irrigated or rainfed land out of which 100 million hectares suffer due to nutrient deficiency or toxicities. Among the toxicities, iron toxicity is well recognized as a nutritional disorder in low land rice production. Iron toxicity is a syndrome of disorder associated with high concentration of Fe²⁺ in soil solution. A wide range of soil types show iron toxicity problem including acid sulphate soils, acid clay soils, peat soil and valley bottom soil. During monsoon rains in the wet season, soluble iron (Fe²⁺) migrate to the surface and its iron content increases to subtoxic or toxic levels.

In rolling topography and with heavy monsoon downpour there is considerable lateral flow of iron containing water from the adjacent uplands to mid – and low lands. This increases the iron content to toxic levels. Poorly drained sandy soils in valleys receiving interflow water from adjacent uplands with laterite horizon (Plinthudults) showed iron toxicity in Srilanka and Kerala and Odisha in India. These soil groups come under the taxonomic orders Alfisols, Ultisols and Oxisols occurring in association with Entisols and Inceptisols. The iron concentration (Fe²⁺) in soil solution that affect low land rice yield ranges from 10 to > 2000 mg L^-1 Iron toxicity induced yield reduction which is associated with poor nutrients . status (deficiency of P, K and Zn) and H₂S toxicities.

Soil conservation in hilly areas

Soil erosion is a major problem on hills because of steep slopes. It has been estimated that about 6 million ha of land requires intensive conservation measures in Himalayan and sub-Himalayan regions of Uttar Pradesh, Uttarakhand, Punjab and Himachal Pradesh. Anthropogenic activities like shifting cultivation, deforestation, over grazing accelerate soil erosion in hilly ecosystem.

1. INTRODUCTION

Fruits and vegetables are increasing in popularity in the daily diet of people of both the developed and developing countries. Product quality and quality evaluation methods are extremely important. The decisions concerning harvesting, maturity, ripeness and quality are based mostly on subjective and visual inspection of the external appearance of the fruits. The quality of fruits and vegetable is mostly based on size, shape, colour, gloss, flavour, firmness, texture, taste and freedom from external and internal defect. Many techniques for evaluating the external quality parameters are now available commercially. Internal quality parameters such as sugar content, acidity, oil content and internal defects however, are difficult to evaluate. Methods are needed to predict the internal quality of fruits and vegetable better without destroying them. Recently, there have been an increasing interest in non-destructive method of quality evaluation and a considerable amount of efforts has been made in this direction. The development of science and technology, especially of information technology has made available many non-destructive methods of analysis of materials which can also be applied to fruits and vegetables. The fruit industry needs non-destructive techniques for online sorting and certifying high quality fruit.

Non-destructive method can be classified according to the principle used to detect fruit properties; mechanical, acoustic, optical and Nuclear Magnetic Resonance (MRI) Techniques.

Pollination

Pollination is the act of transfer of pollen from the male reproductive organ of a plant to the female reproductive organ of same or another plant.

Pollination is of two types

5.1 Self Pollination

It is the transfer of pollen from the anther to the stigma of the same flower from where the anthers have been generated. Thus, self pollination happens to be denominated as autogamy where auto means “same” and gamy means “union”. Obviously, self pollination is found in bisexual flowers. But self pollination also occurs when pollen is transferred from one bisexual flower to the stigma of another bisexual flower of the same plant. This kind of autogamy is known as geitonogamy. So, self pollination has to be defined keeping the parent plant on the main focus not the flower. If genetical consequences are considered, self pollination promotes the occurance of purelines or homozygotes. Self pollinating species developed themselves evolutionary fit due to homozygous balance and do not exhibit significant inbreeding depression.

Oilseed Crops consist of those groups of plants cultivated for substantial quantities of the oil, vegetable fat obtained from the seeds (endosperm) of the plants.

The major oilseeds grown world over are soybeans, sunflowers, rapeseed – mustard (or canola in USA & Canada), groundnuts (peanuts) and linseed. Seed oils from flax (linseed) and castor beans are used for industrial purposes. These Oilseed crops including oilseed bearing trees are the basic biological systems that produce oils, contributing to renewable energy production, help stabilize greenhouse gases, and mitigate the risk of climate change (Jaradat, 2016)

The oilseed crops are of heterogeneous group of plant species not having any common morphological and genetic features except production of seed oil. Crops like groundnut, sesame, castor bean, soybean and niger are grown during the rainy season and the others like rapeseed and mustard and safflower are grown in the post rainy season. Sunflower, however, may be grown in both the seasons. Hence the individual crops forming the conglomerate of Oilseed crops have to be treated differently for their improvement and production. Nevertheless, all of them produce a common end product viz., oil or vegetable fat, the importance of which is growing in product development, commerce and human and farm animal nutrition. The oilseed bearing crops have to be treated as a unique group in terms of research and development to come up with strategies for their judicious exploitation in the national farm economy. From the scientific point of view, the oilseed crops are energy rich and offer impressive potentials in terms of realizable production levels and valuable product development. In India good progress has been achieved so far in research and development of some oilseed crops; but there exists a need to scale to newer heights in terms of realising the hidden potentials of oilseed crops through basic and strategic research, with an integrated approach.

3.1 Introduction

Many gardens follow strict rules. So those are known as formal gardens. Generally formal gardens include:

Italian gardens

French gardens

Persian gardens

Mughal gardens

9.1 Introduction

Xeriscaping is the fine art of creating water-efficient landscapes by using plants that are appropriate to the natural environment. Xeriscaping (zer-i-skaping) is a word originally coined by a special task force of the Denver Water Department, Associated Landscape Contractors of Colorado and Colorado State University to describe landscaping with water conservation as a major objective. The derivation of the word is from the Greek “xeros,” meaning dry, and scape meaning the pattern of the landscape – thus, xeriscaping. The term xeriscaping represents a practice or philosophy with the goal to establish and maintain an attractive and healthy landscape while reducing water usage. One of the prime practices in xeriscaping is the use of drought tolerant plants, but xeriscaping is more than that. It also includes changes in the landscape design, soil improvement, efficient irrigation, mulching, and other maintenance practices which contribute towards a water-wise landscape. But xeriscapes are not dry and dull. Well-designed xeriscapes are enchanting and beautiful. The best xeriscapes use a fraction of the water needed by traditional lawn-dominated landscapes. Some xeric plants require little or no supplemental watering once they are established. And xeriscapes require far less ongoing maintenance.

The Export-Import (EXIM) Committee was constituted in the Seeds Division to deal with application for exports/imports of seeds and planting materials in accordance with the New Policy on Seed Development and EXIM regulation.

The Policy reiterated that all imports of seeds and planting material would be regulated under the Plant Quarantine Order 2003 and amendments made their under, Import licences would be granted by DGFT/PPA on the recommendations of DAC.

The composition of the EXIM Committee is as under: (i) Secretary (A&C) - Chairperson (ii) Agriculture Commissioner (iii) DDG (CS), ICAR (iv) DDG (Hort.), ICAR (v) JS (IC) (vi) JS (PP) (vii) Horticulture Commissioner (viii) Animal Husbandry Commissioner, Deptt. of Animal Husbandry & Dairying (proposed to incorporate by the 94th Exim Committee) (ix) Director, National Bureau of Plant Genetic Resources (NBPGR) (x) Plant Protection Adviser (xi) Economics and Statistical Adviser (xii) JS (Seeds) - Member Secretary

A small quantity of seeds sought to be imported would be given to ICAR, or farms accredited by ICAR, for trial and evaluation for one crop season. On receipt of applications for commercial import, DAC would consider the trial/ evaluation report on the performance of the seed and their resistance to seed/ soil borne diseases. DAC is required to either reject or recommend the application to DGFT for grant of import licence within 30 days of receipt.

Molecular diagnostic laboratory set-up has basic requirements of space, laboratory benches and equipments. In terms of laboratory space, minimum four chambers, one each for sample preparation and nucleic acid extraction, PCR reaction mixture preparation, thermal cycling and gel analysis are required. In addition there should be sufficient space for storage of consumables and laboratory wares. In addition, there are some laboratory equipments which are needed in a molecular diagnostic laboratory. In the following section the equipments of a molecular diagnostic laboratory are discussed. The pictures are taken from the instruments present in the laboratory of the author. The purpose of this chapter is to introduce the reader to a molecular diagnostic laboratory. None of the pictures below are meant for promotion of any particular brand or manufacturer.

3.1 UV-VIS spectrophotometer

A spectrophotometer is required for determining the quality and quantity of nucleic acids extracted from a sample. Photometric research applications such as DNA, RNA and protein analysis can be carried out in a UV-Vis Spectrophotometer. There are various units that feature a broad wavelength range (including UV area), path length correction and a fast reading speed. Various instruments also provide an interface that allows quick measurements directly from the instrument or comprehensive easy-to use software for demanding assays.

The homoeopathic system of medicine is one of the finest systems of cure available in the world. It achieves in totality the ideal laid down by its founder, Dr. Samuel Hahnemann:

"The highest ideal of cure is rapid, gentle and permanent restoration of the health, or removal and annihilation oftlie disease in its whole extent, in the shortest, most reliable, and most harmless way, on easily compreltensible principles".

In homoeopathy, not a single drug is used without knowing its effect on healthy human beings. Hence there is no shooting in the dark. The system operates, by and large, without laboratory tests and other expensive means of diagnosis, which are being used when not needed and that too, unfortunately on patients who can hardly afford the expenses.

Cost wise, there is no medical system in tlie world, which is as inexpensive as homoeopathy. No patient who requires a speedy cure without side effects can ignore homoeopathy; no doctor who has nothing but the good of his patient at heart can overlook the utility and effectiveness of homoeopathy and no Government which has to balance its budget can afford not to use homoeopathy in hospitals and other institutions run for the health and care of the sick and thus it is the most cost effective treatment

The discovery of homoeopathic system of medicine makes a fascinating story.