eChapters

Natural disasters are of meteorological or hydrometeorological origin. Disasters such as earthquakes and tsunamis are occasional, but rainfall-related hazards, flood or drought, are very frequent and thus call for proper monitoring of large-scale rainfall variation, and better manage- ment and planning to combat the problem(s). Variation in rainfall is very large spatially and temporally compared to any other atmospheric parameter, such as pressure or temperature. The large temporal variability in the Indian rainfall has produced frequent flood/ drought and periods of above/below normal rainfall for several centuries past. Areas of high rainfall and runoff are flooded in the summer monsoon season whereas others face water stress and drought conditions. The influence of global warming on rainfall variations over India is reported in numerous observational (Singh et al., 1991, 1992) and modelling studies (Lal, 1994). Though considerable rainwater enters the Indian territory from the Himalayan area, the major hydrological activities and water resources of the country are due to in situ rainfall occurrences, mostly summer monsoon rainfall during June–September. During a normal hydrological wet season the country receives 2911.064 bcm (billion cubic metres) rainwater of which 1856.947 bcm evaporates and 1054.117 bcm enters into surface hydrological processes (surface storage, streamflow/riverflow and deep aquifer storage) (Ranade et al., 2008). Rainfall events display large spatial variability due to variation in intensity, frequency and geographical location of the rain-producing weather systems, such as circular systems (low, depression, tropical cyclone and mid-tropospheric cyclone), linear systems (trough, meander, squall line and convergence zone) and wave disturbances (easterly and westerly waves). Rainfall in other seasons is equally important to monitor, though restricted to some parts of the country, such as winter rainfall over the extreme north, summer thunder showers over the southwest peninsula and northeast India, and post-monsoon rainfall over the south peninsula.

Climate Change has emerged as one of the most serious environmental concerns of recent times. The most imminent climatic change is increase in atmospheric temperature due to increased levels of greenhouse gases such as carbon dioxide (CO₂), methane (CH₄), nitrous oxide (N₂O) and hydrochlorofluoro carbons (HCFCs). Because of increasing concentrations of the greenhouse gases in atmosphere, there is much concern about future changes in the climate system viz., Atmosphere, Lithosphere, Biosphere, Hydrosphere and Cryosphere. Direct or indirect effects on the society linked sectors like water, forestry, agriculture, animal agriculture, fisheries, land and marine biodiversity and health due to climate change/variability are many-fold and economic impact of climate change is enormous. The recurrent drought and desertification threaten seriously the livelihood of over 1.2 billion people who depend on land for most of their needs. The global economy has adversely been influenced due to droughts and floods, cold and heat waves, forest fires, landslips and mudslips, icestorms, duststorms, hailstorms, thunder clouds associated with lightning and cloudbursts, unusual rains and the sea level rise (Fig.1.1). Increase in aerosols (atmospheric pollutants) due to emission of greenhouse gases including black carbon and chlorofluorocarbons (CFCs), hydrochlorofluorocarbons (HCFCs, hydrofluorocarbons (HFCs) and perfluorocarbons (PFCs), Ozone depletion and UV-B filtered radiation, eruption of volcanoes, the “human hand” in deforestation in the form of forest fires and loss of wetlands in the process of imbalanced development are causal factors for climate variability and change. The carbon dioxide (CO₂) concentration was in the steady state at 280 ppm till the pre industrial period (1850). It was rising since then at the alarming rate due to industrialization and burning of fossil fuels and the concentration of carbon dioxide is almost currently touching more than 400 ppm. Global increases in CO₂ concentrations are due primarily to fossil fuel use, with land-use change providing another significant but smaller contribution. The loss of forest cover and wetlands, which normally intercept rainfall and allow it to be absorbed by the soil, causes precipitation to reach across the land, eroding top soil, causing floods and droughts. Paradoxically, the lack of trees also exacerbates drought in dry years by attaining soil to dry out more quickly. For the first time the world over felt the effects of global warming and climate change during the year 1998 in the form of weather related disasters.

We live in a knowledge economy, where ideas generated by talented people and the inventions they make are the new currency. Research and innovation are now seen as the key differentiating factors determining the market value of a product or service. The emphasis today has graduated from cost arbitrage and quality deliverables to Intellectual property (IP) creation and research and development. Intellectual or Intangible property is the creation of human mind. An intellectual property under protection by law is intellectual property right (IPR). Intellectual property is an important business asset giving significant competitive advantages. Intellectual properties are protected by giving time bound restriction to inventor, owner, author, originator, or plant breeder to use, sell, share, or distribute intellectual asset. These are exclusive rights to the owner and give ability for a lawsuit against others for infringement of IP owner. It is also a marketable asset, which can be licensed out to a third party bringing income to the business in the form of license payments and royalties. Intellectual property rights are legal rights, which result from intellectual activity in the industrial, scientific, literary and artistic fields. These rights give statutory expression to the moral and economic rights of creators in their creations. Intellectual property rights safeguard the creators and other producers of intellectual goods and services by granting them certain time-limited rights to control the use made of those productions. These rights also promote creativity, dissemination, application of their results and encourage fair-trading, which contributes to economic and social development.

Vegetables play an important role in human nutrition and health making them important in Indian agricultural economy. The productivity of vegetable crop is seriously affected by several biotic and abiotic stresses and makes the economic conditions of the Indian marginal farmers worst. Under Indian conditions, 25-40% of the total vegetable produce is going waste due to lack of post-harvest storage and processing facilities in the country. Conventional plant breeding methods have improved vegetable crop plants bringing together a wide array of genes responsible for novel productivity and quality traits. However, sometimes, genes needed for crop improvement are not available in the existing germplasm. In genetic engineering, individual genes affecting the traits of economic importance are introduced in commercial cultivars. It is a targeted approach to improve the crop plants, which attempts to avoid the transfer of large blocks of genetic material. It requires identification and cloning of desirable gene and its subsequent transfer in plant species for improvement. Transgenic technologies offer many scientific advantages in breeding for improved vegetable crop quality and performance. However, very few genetically engineered horticultural crops have been released to farmers. As of 2005, one thousand million acres of transgenic crops have been planted globally covering 40% of the United States land area out of which 75% of the area is devoted to cotton, 50% to corn and 85% to soybeans in the United States of America (ISAAA, 2006).

Silk is a way of life in India. Over thousands of years, it has become an inseparable part of Indian culture and tradition. No ritual is complete without silk being used as a wear in some form or the other. Sericulture and Silk Textiles Industry is one of the major sub-sectors comprising the textiles sector. Sericulture is an agro-based cottage industry. Sericulture refers to the mass-scale rearing of silk producing organisms in order to obtain silk. Sericulture is an agro-based labour intensive industry. The major activities involved in a sericulture industry are:

1. Cultivation of silkworm food plants
2. Rearing of silkworms for the production of raw silk
3. Reeling the cocoons for unwinding the silk filament and
4. Other post-cocoon processes such as twisting, dyeing, weaving, printing and finishing.

Plant tissue culture is the cultivation of plant cells, tissues, or organs on specially formulated nutrients media under the right environmental conditions. A whole plant can be regenerated from a single cell. The history of plant tissue culture has been long and started with Haberlandt in 1902 when he proposed the culture of single plant cells, his experimentation never resulted in success. White (1943) became successful in culturing plant cells for the first time, however, Morel (1960) at the Institut National Recherche Agronomique in Versailles near Paris was first to demonstrate meristem culture technology. He successfully introduced tissue culture technology to the orchid industry and produced disease-free plants of grape. By the 1970s, plant tissue culture had become a formidable technology. Plant tissue culture technique has been around for more than 30 years and considered as an important straightforward technology for the production of disease-free high quality planting material and the rapid production of many uniform plants. Its application requires a sterile workplace, nursery, greenhouse and trained manpower.

Plant tissue culturing techniques have been especially important in the agricultural community over the past many years. During this time, plant tissue culture has effectively moved from the confines of small laboratories and has taken its place among broad scale techniques employed by agricultural industry.

COMMON INSECT PESTS AND THEIR CONTROL

Since, insects are the most successful and one among the strongest rivals of man in the competition of existence. Due to their immense tendency of producing numerous viable propagules (eggs) i.e., their high fecundity, their population graph takes a sharp peak in a very short span of time.

A time reaches when the population of an insect type reaches to the level where from it starts causing inconvenience or injury to man, plants, domesticated animals and other human belongings, at such a juncture they are termed as insect pests.

Many, workers are of the view that “insects capable of causing 5 or more than 5% of damage to the crops, stored products, domesticated animals and other human dependences will be termed as pests”. Thus, if an insect causes a loss of less than 5% will not be considered as a pest. Moreover insect pests which cause a damage of 5-10% are said to be minor pests and those which cause a loss of more than 10% of yield are said to be major pests.

Vegetables consist of a large group of plants consumed as food. These represent complex commodities those include roots, stems, leaves, petioles, bulbs, tubers, corms, rhizomes, flowers buds, immature flowers, seedlings, immature and mature fruits and immature, partially mature and mature seeds of plants (Walker, 1988). Vegetables form an important part of human diet, providing essential minerals, vitamins, fibers and very special phytochemicals and they add a variety to the human diet (Singh, 2007). The consumption of vegetables has increased significantly since the consumers have now become more health-conscious (Cruess, 1958; Brackett, 1987a). Vegetables are cultivated in open fields, greenhouses and soil free hydroponic systems. In terms of retail, vegetables can be sold in a fresh or minimally processed form to provide a ready to eat product.

Vegetables are derived from various parts of plants, and it is sometimes useful to associate different vegetables with the parts of plant they represent. The vegetables based on their parts used as food are given below in Table 20.1.

DEFINITION

Pheromones are chemical produced as messengers that affect the behavior of other individuals of insects. The term pheromone was introduced by Peter Karlson and Martin Liischer (1959) and is based on the Greek pherein (to transport) and hormon (to stimulate) is a chemical that triggers a natural behavioural response in another member of the same species. There are alarm pheromones, food trail pheromones, sex pheromones. And many are others that affect behaviour or physiology. Their use among insects has been particularly well documented, although many vertebrates and plants also communicate using pheromones. Pheromones are chemicals released by an organism into its environment enabling it to communicate with other members of its own species.

In India, the total losses of vegetables due to inadequate post-harvest handling, transportation and storage facilities have been estimated at least 25-40%. However, in some vegetables, the post-harvest losses may be as high as 80-100%. Being highly perishable nature of the vegetables, the losses always increase as the produce moves from harvesting to the consumer. Vegetables with a loss of as little as 5% in fresh weight show shrivelled, wilted and staled appearance, which makes the vegetable tissues tough, non-crispy and unpalatable, and eventually, lowers their saleability and consumer acceptability considerably.

Improper harvesting, handling, transportation, storage and distribution of vegetables result in significant post-harvest losses. In such situations, the reduction in post-harvest losses of perishables becomes more essential in countries like India. If these losses are minimised by handling and storing the produce carefully, the following gains can be realised:

Vegetables form an important component of our daily diet as they provide necessary nutrients to the diet and they add colour, texture and flavour to it. Vegetables contain very high content of water (about 78 to 98%) and are reasonably good source of starch, sugar, cellulose, hemicellulose, pentosans and pectic substances. They also contribute minerals, vitamins and indigestible fibers to our diet. The yellow, orange and dark green leafy vegetables are rich source of carotenes- the precursors of vitamin A. Non-volatile acids, such as citric, malic, oxalic and succinic acids, contribute to their flavour. The very strong flavour characteristics of some vegetables like onion, garlic, cabbage, cauliflower, etc. are due to certain sulfur containing volatile compounds. However, vegetables, in general, are poor source of protein and amino acids.

India has made significant progress in the production of vegetables since independence because its diverse agro-climatic zones ranging from tropical to temperate allow the production of a wide spectrum of vegetables.At present, the country is producing about 108 million tonnes of vegetables and considered the second largest producer in the world (Venkatesan et al., 2006). Currently, the plant breeders are focusing their efforts to produce high yielding varieties having resistance against diseases and herbicides and tolerance against drought and salinity. Therefore, the physical aspects of quality, i.e., shape, size, texture, colour, tenderness, etc. are given due priority in the breeding programme with no emphasis to the biochemical and nutritional quality parameters, which include dry matter, proteins, vitamins, sugars, flavouring compounds, alkaloids, flavonoids, etc. However, under present set up of World Trade Organization (WTO), the country will have to compete with quality conscious European and developed countries, and with inferior quality product, it would not be possible to penetrate the foreign market. Therefore, breeding programme in a country like India with future-plans of globalization of agriculture produce must be aimed at achieving good nutritional quality. In different types of vegetable, a varied set of biochemical parameters determines the quality. In this chapter, the methods for qualitative and quantitative determination of these parameters in vegetables have been described.

Locusts are injurious to man as it destroys several human commodities. Locusts are phytophagous and feed on several plants cultivated by man. The locusts are insects belonging to the order orthoptera. The most popular insect is grasshopper. The only difference between the two types of insects is that locust can exist in two different behavioural states whereas grasshoppers do not. When the population density is low locusts behave as individuals, much like grasshopper. As the population density is high locusts gregariously behave as band of nymphs or swarms of adults. The changes in body shape and colour and in fertility, survial and migratory behaviour are accompanied by phase change. The most important locust species is the desert locust (Schistocerca-gregaria). The international pest influences various parts of Asia, Africa and Europe. Other locust species are highly injurious to mainkind like Bombay locust and Indian migratory locust (Locusta- migratoria) found all over India in its solitary phase. The monsoon months in July and August suitable are for locust multiplication conditions. The new generation produced migrate eastward into the Indian desert areas in May and June.

The ultimate aim of production, handling and distribution of vegetables is to satisfy the consumers, and it is well known fact that consumer satisfaction depends on quality of the produce what he gets (Shewfelt et al., 1997). The term quality implies the degree of suitability of produce for a particular use. Quality can be expressed as an absence of defects or degree of excellence of the produce in respect to appearance, i.e., size, shape and colour. Quality is a bundle of attributes that are inherent in the produce and can readily be quantified throughout handling and distribution. It may also be defined as a series of attributes selected based on accuracy and precision of measurement. For grades and standards, the definition of quality is institutionalized so that it may have the same meaning for every one using it. Combination of characteristic features of the produce itself is termed as quality and that the consumer’s perception and response to those characteristic features should be referred to as acceptability.

Quality of produce encompasses sensory properties, such as appearance, texture, odour, aroma, taste and flavour that are readily perceived by the human senses and hidden attributes, such as safety, chemical constituents, nutritive values, mechanical properties, functional property and defects that require sophisticated instruments to measure (Abbott, 1999). Combination of sensory and instrumental measurements of quality attributes provides an estimate of customer acceptability. In a long chain from producer to the actual consumer, each passes judgment and each has his own set of acceptability or quality criteria, often biased by personal expectations and preferences. The component attributes of quality vary with context. The choice of what to measure, how to measure it and what values acceptable to the consumer are determined by the person requiring the measurement, with consideration of the intended use of the produce. Quality can be measured, and it relates to consumer acceptability.

Growth is a characteristic of life of any living being, and it continues until the end of organism. It is expressed in height, weight, volume, size, shape, number and area in every biological system. The shape and size of the plants are because of their continued cellular development and differentiation. Growth, which is quantified mathematically in terms of time, is termed as growth rate, and this gives a valuable information documenting growth as influenced by various abiotic, edaphic and biotic factors.

Principles

Various growth indices are better indicators of plant or crop performance under a specific treatment. Growth indices can be calculated from pot culture or field grown plants. Growth curve can be fitted using these values to predict productivity.

Requirements

Greenhouse or field-grown plants, scale, tags, hot air oven, electronic balance and leaf area meter are the basic requirements for recording the data on different parameters of growth.

Stress is usually defined as an external factor that exerts a disadvantageous influence on the plant. Under both natural and agricultural conditions, plants are exposed to unfavourable environments that result in some degree of stress. Water deficit, chilling and freezing, heat stress and heat shock, salinity, oxygen deficiency and air pollution are major stress factors restricting plant growth. Stress is measured in relation to plant survival, plant processes, growth and crop yield. An environment, which is stressful for one plant, may not be stressful for another, e.g., pea and soybean that grow best at 20 and 30°C, respectively. As the temperature increases, the pea shows sign of heat stress much sooner than soybean, thus, soybean has greater heat stress tolerance.

To the farmers, the plants those survive in an adverse environment are called hardy plants and those, which do not survive under a particular adverse environment, are called susceptible or tender plants. So based on reactions of several plants to the environment, particular plants are selected for a region and also the farmers to suit the nature of a crop adopt different seasons of planting. Biologists have adopted the term stress for any environmental factor potentially unfavourable to a living organism. The ability of the plant to survive the unfavourable factor is due to its stress resistance. There is an equal and opposite reactions for every action. Stress is the action, whereas, strain is the reaction. A body of a plant subjected to stress is in a state of strain. External force of a particular stress causes disturbances in the body of the plant leading to a change in either shape or size and also may lead to internal disturbances, which affect physiological processes. Up to a point, a strain may be completely reversible. When the stress is released at this stage, the plant becomes normal without exhibiting any strain, which is known as elastic strain. Beyond this point, the strain will be partially elastic and irreversible partially, and then we say, it reaches permanent set and it is known as plastic strain.

Drought is one of the most frequent and widely spread stress factors that limit the production of vegetables in the world. Nearly every piece of cultivated land including those in the irrigated areas could be subjected to drought. However, the frequency, duration, severity, timing and extent of drought vary greatly from region to region.

The stress caused by deficit water in the living plant system, which is capable of inducing a potential injury, is called drought stress. Drought can also be defined as scarcity of water for plants and can be due to soil, atmospheric and physiological factors. In our conditions, the great damage to the production of vegetables is brought about by soil drought, which occurs when rapidly available water is spent from zone of the active rhizosphere. That particular moment represents the beginning of drought and as the water reserves in the soil become closer to the lower limit of optimum moisture, the drought becomes more intensive. Under such conditions, plants survive on the limited water available in the active rhizosphere and deeper soil layers, and this water is used to maintain essential life processes at the expense of crop yield.

The lack of precipitation causes drought; the area of cultivable soil in our country under irrigation is very small and so is the possibility to diminish the negative consequences of occasional shortage of precipitation. Aiming to reduce damage caused by drought, a huge number of research-experiments on drought, especially on tolerance has been conducted worldwide. Here, the attempts have been made to review the work done on drought tolerance by national and international centers.

Measurement of soil moisture content is one of the most routinely done soil characterization. Knowledge of soil moisture is essential to understand the behaviour of soil as a medium for plant growth. Moreover, this property of soil helps to explain other processes of the soil. There are several methods of measuring soil moisture content. The most commonly used methods are gravimetric and tensiometric. The standard gravimetric method is quite accurate and simple but is time consuming, cumbersome and destructive. Furthermore, due to relatively small soil samples that are taken, the variability between replicates is great.

Gravimetric Method

Equipment needed includes zip-loc bags or aluminium moisture boxes and soil sampling tools. Soil sampling tools can be a soil probe, auger, or even a post-hole digger. Balance and scale should have accuracy to 0.1 gram. Proper sampling of the field is important to obtain a representative sample of the field. The soil sample should be collected from the field at a minimum of three locations, and the sample locations chosen should represent the entire field. Sampling from the small areas of low spots or ridges that do not represent majority of the field should be avoided. If there are major changes in soil classification within a field, the field should be divided into sub-sites and samples are collected accordingly.

Water is the most abundant compound on the earth but its sufficient availability is always a big constraint in crop production, particularly in arid and semi-arid areas since agriculture is the last priority for good quality water due to severe competition with public health and industrial demand, hence, the judicious and efficient use of water for irrigation in agriculture needs through knowledge of soil-plant-water relationship. The integrated approach for water management in rain fed areas or irrigated agriculture in soil-plant-atmosphere system helps in the selection of suitable crops and varieties and also helps in improving the crop yield and water use efficiency in relation to water supplying capacity of soil profile depending on its texture and rhizosphere depth. Similarly, atmospheric demand of evapotranspiration by various weather parameters via temperature, relative humidity, radiation, rainfall and wind speed is highly variable during the crop season depending on geographical location and is highly area specific. In irrigated area, the saving of irrigation water through improved management practices will further help in extending the irrigated areas with same amount of water, which will further boost agricultural production and sustainability.

Most of the earth’s total water is contained in oceans (96%), a small portion (2%) as snow and ice and the rest (2%) in the water bodies of the continents. Oceans, lakes, rivers and other water bodies of the earth are called hydrosphere. Continuous circulation of water between hydrosphere, atmosphere and lithosphere is known as hydrological cycle (Figure 12.1). Schematic diagram illustrating the earth’s water cycle– the hydrological cycle.

The insect larva after the complete development emerges out from the egg by rupturing the egg shell chorion. It passes through several stages called as instars. Each instar is separated by a moult; the growth from larva to adult involves some degree of morphological changes or metamorphosis. The egg hatches into 1st instar larva which later moults to the second instar larva and so on until at a final moult the adult imago stage. The insect moult takes place after attaining adult except in the insect apterygote insect. In family pantatomide full insect moulting occurs. Many insects especially those with a small number of instars (mosquitoes) and (chrysocoris stolli) insect moulting occurs as Ist, 2nd, 3rd, 4th and 5th imago instar and then after sometime adults insect emerges.

Metamorphosis of Insect: The development process by which first instar immature stage is transformed into the adult is called metamorphosis, which means literally change in the form. The developing stage is morphologically different from the adult. The degree of difference varies from slight to extreme with many intermediates stage. E.g., Cockroaches, Butterfly, Chrysocoris stolli Wolf. The insect may be grouped on dependance of degree of metamorphosis as:

Plant hormone is an organic compound synthesized in one part of the plant body and translocated to another part where in very low concentration it causes a physiological response. However, this definition could not demarcate the vitamins and other growth influencing substances from hormones. For this reason, the term Phytohormone was introduced with included vitamins, growth hormones and flowering hormones. Phytohormone can be defined as an organic substance produced naturally in higher plants, controlling growth or other physiological functions at a site remote from its place of synthesis and active in minute amounts, whereas, an organic substance, which promotes growth, i.e., irreversible increase in height or volume, when applied in low concentration to the shoots of plant is known as growth hormone.

The plant hormones are naturally occurring as they are synthesized by the plant. On the other hand, there are several manufactured chemicals, which often resemble the hormones in physiological action and molecular structures, are called growth regulators. Generally, there are five classes of phytohormones.

The phytohormones universally present in all plants are classified as under:

Most of the plants undergo a period of rest, and in this period, the growth activities temporarily halt. The whole plant looses its aerial parts and undergoes a rest period in the form of rhizome, corm, or tuber. Researchers who study fruit trees use the term rest for dormancy (Samish, 1954). This period is sometimes called as quiescence. The term rest and quiescence can be included under common category dormancy. Some workers differentiate the term dormancy and rest period (Burton, 1964). Wareing and Co-workers (Robinson and Wareing, 1964; Wareing et al., 1964) isolated dormancy inducing substance in sycamore seedlings (Acer pseudoplatanus) which was named as dormin. Further, analysis of this substance by Cornforth et al. (1965) reveals that dormin was identical to Abscisin-II (a substance speeding the abscission of the young flowers of Lupinus luteus and have antiauxin effect).

Dormancy can be defined as an arrest in development of buds, seeds, embryos, or spores under conditions otherwise suited for growth (Taylorson and Hendricks, 1977). It is a survival strategy (exhibited by many plant species), which enables them to survive in climates where part of the year such as winter or dry seasons is unsuitable for growth. The dormancy may be due to the factors within the dormant organ or seed itself and it is called spontaneous dormancy. This type of dormancy is found in seeds, buds and perenating organs like tubers, bulbs, bulbils, corms and rhizomes. In some weeds, dormancy is caused by high or low temperature and this type of dormancy is called as imposed dormancy. Seeds normally germinate in darkness but are inhibited by light are considered to become dormant after exposure to light (Bewley and Black, 1982; 1985). Seeds that require light for germination are said to be photo dormant.

Efficiency of pest control by insecticides is dependent on the correct type of pesticide formulation applied at the proper time and correct dosage, besides the good equipment. Equipment used to apply insecticides must be suited to the crop (or other situation in which the pest is to be controlled), to the scale of operation, and to the formulation of chemical to be applied. In the long run, it is good economy to buy the best equipment available.

Application equipment of high quality means not only good, durable construction but also good design that gives it a professional appearance. Because it is desirable and often necessary to utilize pesticides in a variety of ways, various types of dispensing equipment have been developed. This equipment is placed in the following categories: (1) Sprayers, (2) Dusters, and (3) Granule Applicators. In addition, specialised equipment of various sorts (such as mist machines and fog generators) are available for particular purposes. Since detailed consideration of pesticide application equipment is out of the scope of this book, only a brief consideration is given here to some important features of the main types of equipment.

Fresh fruits are an important component of our diet since they provide essential nutrients like minerals and vitamins and are often attractive to the consumer because of their taste, texture, colour, aroma and flavour. Fruits of different species are botanically different, and they may be derived from various parts of the flower, including the pericarp, mesocarp and receptacle. Growth of fruit tissues is initiated shortly after fertilization and accompanied by the accumulation of food reserves donated by the parent plant. Fruit ripening corresponds to a number of physiological, structural and biochemical changes, which make the fruit attractive to the consumers. These changes include physical changes, viz. the changes in colour, texture, taste, aroma and flavour, and the metabolic changes such as changes in the level of enzymes and gene expression. The varieties of mechanism, which exist in different fruits to bring about a series of these changes to make them attractive for consumption, have been summarized in Table 9.1.

Because of the variety of ways in which these quality attributes are generated, there are significant differences existing in enzymes involved in ripening of different types of fruit. Once initiated, these physiological and biochemical modifications continue until the fruit is in peak condition for consumption. If the fruits are not harvested at appropriate stage of maturity, these changes continue and make them more susceptible to mechanical damage, infection and senescence. Since harvesting fruits at right stage of their maturity is critical, it is important to understand the biochemistry of fruit ripening process fully. This chapter will highlight the biochemical and metabolic changes involved during fruit ripening to make the fruit acceptable for potential consumers. Some of the possible genetic manipulations, which can be done to delay the onset of these ripening related changes, are given in later part of the chapter.

The original divisions of the class insecta are a matter of dispute and it is not easy to decide which division to use many, different original order and groups are recognised by different experts and which one is best will no doubt remain problem. The words taxonomy, systematics and classification have been used alternatively for the same subject by various scientists. However Simpson 1964 has attempted to segregate these. According to him classification is the order of animals into groups on the basis of their relationship that is association of contiguity similarity or both. Systematic is the scientific study of the kinds and diversity of organism and of any and all relationships among them, while taxonomy is the theoretical study of classification including its bases, principal procedures and rule. All animal species are segregated into small and large groups on the basis of their comparative similarities and dissimilarities. The largest group is called phyllum as followed by class, order, family, genus-species and sub-species respectively. The Phylum, class, order, genus, species etc. The division of class insecta into primitive wingless insects, the apterygota and the secondary winged-insects the pterygota and included some orders. The class insecta may be divided into two subclasses.

In addition to pathogenic diseases, numerous abiotic disorders may also arise during growth, development and post-harvest storage of solanaceous vegetables. The physiological disorders of vegetables that have been included in this chapter are directly or indirectly associated with genetic, cultural, nutritional, physiological and environmental factors, and in most of the cases, the definite cause of disorder is not well understood due to the association of numerous factors together. These disorders may be related to colour, shape and form/condition of the produce. The genesis of physiological maladies was earlier poorly understand due to complexity in their characteristic symptoms but at present is relatively more transparent. The characteristic symptoms of abiotic disorders discussed below have not been attributed solely to a single agent or to a single cultural or environmental factor. The environmental factors correlated with the incidence of these disorders include high light intensities and low or high temperature of that growing area. For many abiotic disorders, very limited research work has been done, and the causes are poorly understood both in terms of why cultivars differ in susceptibility and why certain environmental factors predispose the plants or parts of the plant to the disorder. The growers feel concerned with the incidence of abiotic disorders as these cause huge economic loss. Therefore, the control of these abiotic disorders is essential for profitable production of solanaceous vegetable crops. In this chapter, emphasis has been given on practical aspects of diagnosis and potential control measures.

Many insect can be observed at any hour of the day. This is proper understanding of the taxonomy and biology. The insect collection, storage and preservation are useful technique of their culture. The best period for collection is from early spring to late rainfall and the best time of the collection of most of the species is during the daytime. The insect are polyphagous and therefore, plants provide one of the best collecting places. Insect can be picked, swept off the plant with the help of a net. Different species feed on different kinds of plant, one should therefore, examine all sorts of plant, flowers weed shrubs and trees. Few insect are only found in the stem, bark, wood, fruit or roots etc. Some species can be found in the leaf mud and litter on the surface of the soil, particularly in woods or areas where the vegetation is dense, others can be found under stones bark etc. other house hold insects may be observed feeding on clothing, furniture, grain, food and other parts of Plant and House and some are aquatic insects.

Fruits provide a suitable environment for seed maturation and often a mechanism for the dispersal of mature seeds. By anatomical definition, fruit is a mature ovary, and therefore, includes carpel tissues in part or in whole. However, many fleshy fruit species develop mature fruit tissues by including extra-carpellary floral components. The examples include strawberry, pineapple, mulberry and pome fruit (apple, pear, etc.) in which the receptacle, bracts, calyx and floral tube (the fused base of floral organs), respectively constitute majority of mature fruit tissues. Even species with fruit derived from carpel tissue exclusively can display a range of developmental programs, spanning the relatively uniform single expanded carpel or drupe of stone fruit to the differentiated carpel tissues, giving rise to the peel (flavedo) and multi-carpel flesh as observed in citrus and banana. Although genetic selection of agriculturally valuable fruits is being done since centuries but most of the information about how fruits develop, how this development is coordinated with embryonic development and seed formation and the molecular, cellular and physiological events that control fruit growth and differentiation are still lacking. In the following sections, the various aspects controlling fruit setting and development have been discussed.

The term ecology was first of all proposed by zoologist H. Pietir in 1865. It was derived from the Greek word ecologic means oikos = living place and logos = study and some definitions of ecology presented below:

1. According to Ernst Haeckel, 1869 “The total relations of the animal to both its organic and inorganic environment is called as ecology”.
2. E. Warming 1895 “Ecology is the study of animals and plants in relation to their environment”.
3. According to Odum 1963 “Study of structures and functions of nature is defined as ecology”.
4. Krebs 1985 says that the “Ecology is the scientific study of interaction that determine the distribution and abundance of organisms”.

The life of insects is dependent on the adaptability of the environment, if the environment is unfavourable then the insect population ceases and vice-versa. This insect move one place to another to get the favourable environment so that they can pull on their life activities properly. There is a deep relationship between the living community and non-living environment. Both are affecting each other greatly. This inter-relationship between living community and non-living environment is called as ecosystem. The term ecosystem was first used by A.G. Tansley as early as 1935 and he defined, “Ecosystem is the system of resulting from the interaction of all the living and non-living factors of the environment”.

Flowers are the reproductive structures of Angiosperms. The apical shoot meristem of the vegetative plant continues to produce new leaves and vegetative buds, which may develop into branches, but after sometime, the meristem, instead of producing leaves and branches, becomes transformed into specialized reproductive structures called flowers. The transformation of vegetative shoot apex into re-productive structure is called flowering. The process of flowering is controlled by two main important environmental factors, i.e., (i) the duration of light received by the plant called photoperiod and (ii) the low temperature treatment given to the seed or the plant referred to as vernalization (Figure 6.1).

The transition from vegetative to floral meristems in higher plants is programmed by the coincidence of internal and environmental signals. The timing of floral transition has a direct impact on reproductive success. One of the most important environmental factors that affect the transition is the change in day length (photoperiod). Classical experiments imply that plants monitor photoperiods in the leaf and transmit that information coded within an elusive signal dubbed florigen to the apex to reprogram development. Recent advances in Arabidopsis research indicate that the core of the day-length measurement mechanism lies in the circadian regulation of CONSTANS (CO) expression and the subsequent photoperiodic induction of the expression of FLOWERING LOCUS T (FT) gene, which might encode a major component of florigen. The florigen paradigm was conceived in photoperiod-sensitive plants; nevertheless, it implies that although it is activated by different stimuli in different flowering systems but the signal is common to all plants. Flowering time, the major regulatory transition of plant sequential development, is modulated by multiple endogenous and environmental factors.

Respiration is the oxidative breakdown of complex substrate molecules such as starch, sugars, and organic acids normally present in plant cells to simpler molecules such as carbon dioxide and water. Concomitant with this catabolic reaction is the production of energy and intermediate molecules that are required to sustain the myriad of metabolic reactions essential for the maintenance of cellular organization and membrane integrity of living cells. Since respiration rate is so tightly coupled to the rate of metabolism, the measurements of respiration provide an easy non-destructive means of monitoring the metabolic and physiological state of tissues, e.g., the events of senescence and ripening are often signaled by abrupt changes in respiration.

Maintaining a supply of high-energy compounds like adenosine triphosphate (ATP), nicotinamide adenine dinucleotide (NADH) and pyrophosphate (PPi) is a primary function of respiration. Nicotinamide adenine dinucleotide plays very important role in mitochondrial matrix (Moller and Ramusson, 1998). The overall process of aerobic respiration involves regeneration of ATP from adenosine diphosphate (ADP) and inorganic phosphate (P_i) with the release of carbon dioxide and water. If glucose is used diphosphatas substrate, the overall equation for respiration can be written as follows:

Photosynthesis- the word photo refers to light and synthesis means preparation. Thus, photosynthesis is the process by which the green plants use light energy of the sun to synthesize carbohydrates. Carbohydrates like simple sugars (glucose) can be stored as starch.

The green plants are eaten by the herbivores, which in turn are consumed by carnivores. Ultimately, the decomposers derive their nutrition from the dead plants and animals. Thus, all organisms are dependent directly or indirectly on green plants, which synthesize food by photosynthesis. Thus, photosynthesis is the most important life sustaining process of nature (Calvin, 1976; Emerson and Arnold, 1932). Photosynthesis is a series of biochemical reactions, which can be essentially summarized as follows:

The capacity to reproduce is one of the most important characteristics of all living beings. Reproduction is a process of producing offspring and is aimed to preserve individual species. This is known for self-perpetuation. There are several modes of reproduction, which vary from species to species, in available conditions. All the reproductive methods of plants are broadly classified into two types, i.e., (i) asexual reproduction (apomixis) and (ii) sexual reproduction (amphimixis). In asexual reproduction, the new individuals are produced by any means other than the fusion of sex gametes. In sexual reproduction, the gametes from male (androecium) and female organs (gynoecium) of the flower are fused to produce a zygote (Schranz et al., 2005; Rodrigues et al., 2008). Amphmixis and apomixis are the two sides of same coin (Curtis and Grossniklaus, 2007).

Plant body consists of numerous microscopic box like components called cells. The word cell has been derived from the Latin word cellula, meaning a small room. The descriptive term for this smallest living biological structure was coined by Robert Hooke in a book he published in 1665. When he compared the cork cells, he saw through his microscope to the small rooms monks lived in. The cell is the basic structural and functional unit of all known living organisms. It is the smallest unit of life, which is classified as a living thing and is often called the building block of life (Maton et al., 1997). It is defined as a mass of protoplasm surrounded by a membrane- the plasma membrane- within which complicated chemical reactions are going on.

Exocrine Glands Types: Exocrine glands are generally of ectodermal origin and are widely scattered over the insect. Exocrine glands release their secreations through ducts into the lumen of various internal organs. Example: Accessory reproductive glands, salivary gland, silk gland. Gland is mainly composed of secretory epithelial cells. These are simple unicellular, bicellular, multicellular, complex multicellular (compound glands). These glands are ectodermal in origin and are secreated over the insect body.

Examples. Wax glands, lac glands, cephalic glands, poison glands.

1. Glands pouring (secreting structural materials). Some insects secrete substances that are used in building their houses (beehive) as protecting them (lac or cocoon).

In insects nervous system is highly developed and serves to coordinate the activities of its various systems. Units of this system are known as neurons. Which carry information in the form of electrical impulses from external and internal sensilla (Sensory cells) to appropriate effectors e.g., glands, muscles and glail cells. Nervous system of insects may be divided into three parts.

1. Central nervous system
2. Sympathetic or visceral nervous system
3. Peripheral nervous system.

The respiratory system or tracheal system is involved in gaseous exchange in the insect with the environment. In this system there is a system of internal tubes, the tracheae that directly transport the oxygen to the parts of the body. Therefore, it does not use the circulatory system as the vehicle for gaseous exchange. The trachae open outside through segmental pores called spiracles having some system of closing and opening. Except proturans and some collembolans all insects possess tracheal system for respiration. These insects live in moist habitats where gaseous exchange takes place directly with the environment via the integument.

Insects show sexual Dimorphism i.e., the sexes are usually separate and are dioecious. Male reproductive system produces sperms, stores them and delivers them i.e., spermatozoa. It also produces the seminal fluid, which provides a suitable environment to the sperms and nourishes them. Male reproductive system also situmilates are the female for oviposition. The female reproductive system produces and stores eggs provide nutrition to them for embryonic development, recieves and stores the sperms, is a site of fertilization. Female reproductive system also deposite eggs and may provide additional protection to the embryos. The reproductive system of both male and female insect’s bearis a gononpore, which is an opening of a pair of gonads. Both the gonads open into the gonopore by a median duct. For the secondry sexual purposes, e.g., formation of spermatophore and egg-case or ootheca, some accessory glands are associated with the reproductive system.

Excretory system of an organism maintains its homeostasis i.e., a constant internal environment. Here in insects, the blood is haemolymph therefore it determines the excretion of an insect’s body. Excretion also eliminates the nitrogenous wastes and maintains the salt- water balance and also regulates the uniformily of haemolymph. The characteristic feature of insects is the presence of malpighian tubules to eliminate the metabolic wastes, whereas rectum walls carry out the function of reabsorption of salt and water. A minimum amount of water is excreted with nitrogen waste in order to conserve the water in body.

Insects, belonging to the phyllum-arthropoda, have an open circulatory system. An open circulatory system is that in which blood bathes the organs rather internal organs directly in the haemocoel, the coelom of the insects, which actually is not a true coelom. Almost entire haemocoel of insects is formed from the epineural sinus. The haemocoel is divided into the perineural sinus and perivisceral sinus by the dorsal and ventral diaphragm respectively. The characteristic of insects is the presence of dorsal vessel, which comprises of anterior aorta and a posterior heart and is the only conducting tube present in insects. The blood of insects mostly does not perform gaseous exchange, therefore it does not contain haemoglobin, and blood in insects is called the haemolymph, which is red-coloured. So in order to study the circulatory system of insects, the major focus must be on the dorsal vessel, which has been described as follows, along with its accessory structures.

The digestive system of insects comprises of two main sub-parts, one is the alimentary tract or also known as the digestive tract and the associated digestive glands. These digestive glands may be associated directly or indirectly such as, salivary glands, gastric, caeca (directly) and Malpighian tubules (indirect). If we have to describe an insect and its anatomy, or its histology, it is based on the orthopteroid plan, which is mostly shown by grasshoppers, crickets, and locusts, because their plan shows and describes the basic, primitive design from which other groups of insects have enolved and showed modifications and specialization. We can say that this orthoptera insect shows the general anatomical and histological features of a generalised insect.Today, we see that insects have invaded and adapted in almost all types of habitats and the major reason for their biological success is their ability to show digestion, utilization and their ability to eat an extremely vast diversity of food. It is due to this abilityof extremizm in food diversity that insects have undergone many specializations and modifications of the alimentary canal. What modifications a species of insects undergoes, depends upon the type of insects, depends upon the type of food it consumes. The diversity is also seen in the way of obtaining, storing, processing and absorbing the food. These differences may be structural or functional or both. It is not necessary that these differences must be present in different species only, those may be shown by different life stages of a single insect, by two sexes of a single species, etc. e.g., the difference between the mouths parts of mosquito species.

The dairy processing industry in India deals mainly with the fluid milk and/or short shelf life products, despite India being the largest producer of milk in the world, with an output bordering the 110 million ton mark. This feature offers immense potential in the area of R&D in dairy processing in terms of value addition. The potential gains are many, including higher returns for producers and advantages in terms of health, therapeutic, nutritional and/or gastronomy for the consumers. However, the Indian Dairy Industry can amass possible benefits by being a global contender in trade only when its products are at par with those available in the rest of the world in terms of price and quality. It is evident from the post-WTO scenario that for sustainability, Indian dairy industry will need to maintain wholehearted commitment to quality management right from the farm to the consumer, shedding its inhibitions and resistance to changes.

9.1. WTO Agreements and Food Industry

The General Agreement on Tariffs and Trade (GATT) was formed in 1947 and lasted until 1994, when it was replaced by the World Trade Organization (WTO) in 1995. The WTO is the only global international organization dealing with the rules of trade between nations. It provides a forum for negotiating agreements aimed at reducing obstacles to international trade and ensuring fair deal for all, thus contributing to economic growth and development. Currently, there are 16 agreements to which all WTO members are parties and two agreements to which only some WTO members are parties. WTO currently has 153 members, of which 117 are developing countries or separate customs territories. The Head Quarters of WTO is at Geneva (Switzerland) and the official languages are English, French and Spanish.

India has achieved a commendable position of being the highest milk producing country in the world. Nearly 110 million tons of milk per annum is contributed by small and marginal farmers having 2-3 milch animals which are scattered in lakhs of villages across the length and breath of India. The poor socio-economic conditions are serious constraints in producing raw milk of good quality. The quality of milk is further deteriorated during subsequent handling and transportation. Milk being a product of biological origin, is extremely vulnerable to attack by microbes. It is also a good vehicle for additives and adulterants without any apparent changes in its appearance. Thus, milk is susceptible to several post-secretion changes, some natural and some man-made.

Indian dairy industry faces a mammoth task to meet the world quality standards, particularly in the present context of globalization and increasing quality conscious consumers. In view of the highly perishable nature of milk soon after it is secreted, it is important to monitor its quality by having a firmly installed quality assurance programme along the entire production-to-supply chain. This can be possible with application of sensitive, accurate and rapid methodologies as a tool in quality assurance programmes.

Quality comprises of several attributes ranging from physical, chemical, technological and bacteriological through nutritional to aesthetic. All dairy plants have a quality assurance laboratory (Fig. 8.1) and usually follow chemical quality control with the intention of payments to contractors/producers, processing of milk and meeting mandatory requirements for end products. Quality is judged both by objective and subjective tests.

Cleaning and sanitisation are the two processes employed in dairy industry to ensure hygiene and product safety. The safety of milk and milk products is a very important aspect and every attempt should be made, during processing, to minimize contamination through handling and processing equipments. The accumulated dried milk solids (milk stones) consist largely of calcium phosphate, milk proteins (precipitated, coagulated and baked on by heat) and insoluble calcium salts from water and washing detergents. The surface can also contain biofilms, which are formed by the incorporation of bacteria into the accumulated inorganic and organic materials that attach to most surfaces. With time and in the presence of nutrients, a biofilm and the bacteria within the biofilm will grow and become strongly attached to the surface. The removal of dairy soil, milk stones as well biofilms is the prime objective of the cleaning process. International Dairy Federation (IDF) defines a clean surface as one which is free from residual film or soil that will not contaminate the food products coming in contact with it.

While cleaning refers to the removal of soil from the surface of the equipment, sanitisation implies destruction of all pathogenic and almost all non-pathogenic organisms. Cleaning and sanitisation are complimentary processes. Either of the processes alone will not achieve the desired result, which is to leave the surface as free as possible from soil and viable organisms. In actual practice cleaning and sanitisation overlap to some extent, as during cleaning a high proportion of bacteria are destroyed and others get removed in the detergent solution. Inadequate or faulty cleaning and sanitisation can have serious consequences, as milk is a prefect nutrient in which bacteria can multiply. A thorough understanding of the cleaning methods is, therefore, necessary for economic production of high quality food products.

Packaging is a tool that is used to contain the product, protect the product from environmental hazards and help in marketing the product. Examples of ideal natural packaging systems are that of a banana, orange, coconut and egg, where the packaging is provided by nature. The major step forward in the food processing arena has been the ability to protect and preserve products with packaging and hence ensuring the availability of fresh and processed products out of season and over long distances in various forms. Milk is a highly perishable product and is not uniformly available round the year to the whole country due to geographical and genetic variations. If dairy products are to be available all the year round the country, irrespective of the season and area, it must be presented in packages that are convenient to purchase and use.

Packaging has additional responsibilities of providing convenience, safety and facilitating, besides handling, storage and distribution of products. It should prevent spoilage of food by microbial or insect attack. It also preserves the quality and nutritive value of many foods by the exclusion of oxygen and the control of moisture loss or gain. It increases the shelf-life and ensures longer availability and contributes to the product image through structural and graphic design. Product information is also given out through the medium of labeling, which acts as a tool for marketing and advertising.

Milk, as secreted in the udder of a healthy animal is practically sterile, but during its transit to the processing plant, becomes heavily contaminated with a variety of micro-organisms. These may be responsible for spoilage of milk or for causing diseases in animals and human beings. It thus becomes imperative that adequate heat treatment is given to milk to destroy these micro-organisms, thus increasing the shelf-life of milk as well as making it safe. Varied heat treatments offer the potential of producing different kinds of processed milk to satisfy the needs of the consumer. To cater to the ever-increasing demand of consumers, several varieties of processed milk are available in the market, some of which are discussed below.

5.1. Pasteurised Milk

Pasteurisation was named after the renowned French scientist Louis Pasteur, who discovered that spoilage organisms could be inactivated in wine by applying heat at temperatures below its boiling point. The process was later applied to milk and remains the most important operation in the processing of milk.

It is the process by which every particle of milk is heated to such temperature-time combinations (usually to 63°C for not less than 30 minutes or to 72°C for not less than 15 seconds) and promptly cooled to 4°C or below so as to destroy any harmful bacteria that may be present without affecting flavour and food value. Pasteurisation does not involve the use of any additives. Apart from making milk safe to drink, pasteurisation increases the length of time it can be kept before it spoils. The nutrient losses due to pasteurisation are so small, that they are considered insignificant.

Milk, on reception at the dairy factory is subjected to various unit operations on its way to product manufacture. Processing is applied as a means to convert liquid milk into products or to render the milk (and products) safe from harmful organisms. The basic processing operations to which milk is subjected at processing units have been described in this chapter. The common processing operations employed are depicted in Fig. 4.1. Several of these operations are routine compulsory processes common to all dairies, while certain others are undertaken for specialized purposes in some dairies.

4.1. Filtration and Clarification

At the time of milking various foreign materials such as dirt, feed, manure, dandruff, hair, flies, dust particles, worn out cells, cell debris and many other undesirable substances may find their way into milk. The process of removal of this extraneous matter (solid particles of higher density than that of milk) is called filtration or clarification. This can be achieved either by simple filtration through specially woven filter cloth or continuous process of centrifugal clarification that helps

Milk production in India is a subsidiary activity of agriculture in contrast to the organized dairy sector in the western countries. Farmers and landless labour maintain one to five milch animals. As a result, small quantities of milk are produced in a scattered manner. Due to this reason, milk procurement models from western countries, such as bulk cooling and bulk transportation are not applicable under Indian conditions. Collection of small amounts of milk is scattered over long distances and, therefore, poses a formidable challenge in maintaining the quality attributes and keeping costs down.

3.1. Rural Milk Collection

A systematic approach to rural milk collection suitable for the tropical climate and techno-economic conditions prevailing in India has been developed, based on the indigenous experience gained over the past few decades. In the first phase, extensive surveys are undertaken in the milkshed areas, where the milk plant is to be established. The second phase involves ‘route planning’ taking into account, availability of quantities of milk, access to roads for plying vehicles and distance from the site of dairy plant. Then zones are identified, representing equal costs of collection and transportation. In the third phase, planning is done for locating the primary collection centres as well as chilling centres, where, milk can be cooled to 4ºC before transporting to the milk plant. Milk may be collected from individual producers either by the contractor or by forming village level cooperative societies.

Milk is the secretion of the mammary glands of animals. For human consumption, milk is that liquid obtained from the cow or the buffalo during the period following at least 72 hours after calving or until the milk is colostrums-free. It is a complex biological fluid consisting of seven main components: water, fat, protein, sugar (lactose), minerals, vitamins and enzymes. Milk is a white opaque fluid in which fat is present as an emulsion, protein and some mineral matters in colloidal suspension and lactose together with some minerals and soluble proteins in true solution. The opacity of milk is due to its content of suspended particles of fat, proteins and certain minerals. The colour varies from white to yellow depending on the carotene content of the fat. Skimmed milk is more transparent with a slightly bluish tinge. Milk of a good flavour has a pleasant, slightly sweet taste, and no odour. It is an excellent source of calcium, phosphorous and riboflavin. Vitamin A and D are currently added to whole milk, partly skimmed and skimmed milk.

1.1. Introduction

The significance of milk as the most wholesome single food available in nature has never been debated. It is as ancient as mankind itself, as it is the substance created to feed the mammalian infant. All species of mammals, from the lowly rat to the mighty whale, produce milk for this purpose. Several centuries ago, perhaps as early as 6000-8000 BC, ancient man learned to domesticate species of animals for obtaining their milk for consumption. These included cows, buffalos, sheep, goats and camels, all of which are still used in various parts of the world for the production of milk for human use (Fig. 1.1 & 1.2).