eChapters

2.1 Introduction

Dear students, you shall be taught plant biotechnology during the third semester in the course ‘Elementary Plant Biotechnology’. But, for understanding of non-conventional breeding methods/techniques you should acquire some knowledge of basic plant biotechnology. Hence, in this chapter plant biotechnology is introduced to you in brief.

2.2 Definition

The term ‘biotechnology’ stems from fusion of ‘biology’ and ‘technology’. It was coined by Karl Ereky, a Hungarian agricultural engineer, in the beginning of 20th century. According to him, all processes used to yield finished products from raw materials with the help of living organism come under biotechnology. It may be defined in number of ways. However, in simple terms, biotechnology may be defined as ‘the controlled use of biological agents, such as microorganisms, or cellular components, for beneficial use’ (U.S. National Science Foundation).

8.1 Introduction

In pre-historic times, people lived as hunters and gatherers. Then, gradually they began to produce the crops they ate on piece of land, and farming was born. Wild plants have certain traits that keep them protected and productive without human intervention. However, most of them are not desired for food or other use by humans. By spontaneous mutagenesis and hybridization, occasionally variant plants were found that lacked some of the undesired traits, such as bitter taste, thorns, or had particularly attractive features, such as larger fruits and more regular tuber shape. Consequently, as time passes such variation was picked up by humans by selection of such variants, keeping and advancing them. Thus, plant domestication is the process of bringing wild species under human management. In other words, plant domestication is the process by which genetic changes (shifts) in wild plants are brought about through a selection process imposed by humans. It is a method of plant breeding in the sense that, when successful, it provides domestic types that are superior to ones previously available.

Pre-historic man domesticated virtually all of our present-day crops. But, domestication of wild species continues even today and is likely to continue for a long time in the future. For example, in many countries there is an increasing emphasis on bringing forest trees producing timber, etc. under human management. A similar trend is seen for medicinal plants and plants producing specific compounds of interest, e.g., hydrocarbons, oils, etc. Kala jeera (Bunium persicum), a perennial spice, was domesticated during 1990s in Himachal Pradesh; it is being cultivated as an orchard crop. One important aspect of domestication at present is the use of particular genes from wild relatives in the improvement of cultivated species. When a plant breeder transfers one or a few desirable genes from a wild relative to a cultivated type, he is, in a sense, domesticating the wild species in part.

11.1 Introduction

In chapter 10, we have discussed the different types of variation, namely, genetic, environmental and genotype × environment interaction variations. We have also learnt how to estimate the phenotypic variance and its components including genotypic (also called genetic), environmental and g × e interaction variances from trial data. Now in this chapter, we shall discuss on the genetic components of polygenic (quantitative) variation and its implications on breeding strategies.

11.2 Genetic Components of Polygenic Variation

In this chapter whenever we call genetic variation, we mean polygenic (quantitative) variation, and we know that variation is usually measured in term of variance. However, variation, variability and variance are almost used synonymously. The genetic variance has been classified into different components by various workers. Fisher (1918) divided genetic variance into three components: additive, dominance and epistatic (interaction) variances. Wright (1935) classified genetic variance into two components: additive variance and non-additive variance (dominance and epistatic variances). Mather (1949) classified genetic variance into two components: heritable fixable variance (additive variance and additive × additive epistatic variance) and heritable non-fixable variance (dominance variance, and additive × dominance and dominance × dominance epistatic variances). Later, Hayman and Mather further divided the epistatic variance into three categories: additive × additive, additive × dominance and dominance × dominance.

12.1 Introduction

Allowing certain plants from the population to reproduce, while barring others, comprises selection. This permission for reproduction may be granted by nature or by the plant breeder. In the other words, both nature and breeder exert selection pressure. Natural selection operates through differences of fertility among the adult individuals of parent generation as well as through differences of viability among the individuals of offspring generation. Artificial selection, i.e., the selection from action of the plant breeder in the choice of parents, produces its change of gene frequency by separating the adult individuals of parent generation into two groups, viz., the selected and the discarded, that differ in gene frequency. So, selection simply implies the retaining of desirable types or culling of unwanted or undesirable types.

13.1 Introduction

F₁ plants produced by crossing two parents with contrasting characters are called hybrids. Hybrids generally exhibit increased vigour and fitness over the parents. This phenomenon is called hybrid vigour or heterosis. Thus, heterosis is the phenomenon in which the hybrid of two genetically dissimilar parents shows increased vigour at least over the mid-parental value. Heterosis is just the reverse of inbreeding depression, which results after selfing the plants of the cross-pollinated crops. Kolreuter (1761-66) was the first to report hybrid vigour in the hybrids of tobacco, Datura, etc. Most of the early plant hybridizers including Mendel (1865) had noticed this phenomenon. Shull (1914) coined the term heterosis from two Greek words, hetero (different) and osis (condition), to replace his own earlier word ‘stimulus of heterozygosis’. In current usage, heterosis and hybrid vigour are used as synonyms and interchangeable.

13.2 Types of Heterosis

Depending on the nature, origin, adaptability, reproducibility and non-reproducibility, heterosis can be categorized into two: (1) euheterosis (true heterosis) and (2) pseudo-heterosis (false heterosis, i.e., luxuriance).

14.1 Introduction

The mating or crossing of genetically dissimilar individuals is called hybridization. Hybridization results in recombination that in turn widens the spectrum of variation among individuals. So, the reason for genetic diversity in progenies of crosses is the formation of new combinations of genes. This newly created variability is then channelized into development of better cultivars.

The importance of pure line selection as a method of breeding started decreasing when plant breeders felt that the genetic variability existing in the land races of self-pollinated crops had been almost completely exploited and there was little chance for further improvement in these crops by using pure line breeding. Therefore, no option was left for plant breeders except to cross diverse individuals and combine together desirable characters in a single genotype from two or more parents. The planned hybridization which could avoid self-pollination and chance cross-pollination, soon became the main feature of almost all plant breeding programmes.

20.1 Introduction

A hybrid cultivar (also called hybrid) is the F₁ progeny of a planned cross between inbreds, cultivars, clones or populations. Depending upon the breeding approach, the hybrid cultivar may involve two or more parents. A critical requirement of hybrid production is that the parents are divergent. This divergence is the cause of superior performance of hybrids. The outstanding performance of crops is due to the exploitation of the phenomenon of heterosis (hybrid vigour) which is high when parents are divergent. Much of what we know about heterosis breeding (also called hybrid breeding) came from the work on maize hybrid cultivar development. However, commercial hybrids are now available in many crops including self-pollinated crops.

21.1 Introduction

There are a number of field, vegetable, fruit and ornamental crops that are cultivated using asexual or vegetative parts such as stems, roots, modified flower etc. This is a heterogeneous group of plants and can be further categorized into four groups for ease in understanding the breeding methods applied to them. They are:

Group 1: Flowerless or Sterile

This group either does not bear flowers or if bears, they are sterile and do not set seed, e.g., ginger, mango ginger, turmeric, garlic, betel, many yams, etc.

Group 2: Apomicts

Apomixis has been reported in over 300 genera. But stable apomixis has been reported only in a few cultivated plants like meadow grass, citrus, mango, raspberry, etc.

Group 3: Flowers but Seed-Set Rare

In this group flowering takes place under specific situations but seed-set is limited, e.g., potato, sweet potato, sugarcane, etc.

29.1 Introduction

An environmental factor that interferes with the complete expression of genotypic potential of a crop is called stress. The stresses may be biotic (pathogens, insect-pests, etc.) or abiotic. The main abiotic stresses are drought, mineral deficiency/toxicity and heat or cold. The losses due to abiotic stresses can be minimized by:

Crop management: The degree of stress can be reduced by suitable agronomic/soil management practices, e.g., irrigation to relieve drought stress, soil amendments to reduce salinity.

Development of resistant cultivars: This offers the cheapest, easiest to apply and most eco-friendly approach to reduce losses due to any stress.

Indirect breeding: Evaluation of materials under stress often helps in identification of a resistant line that may become an important commercial cultivar.

???????Direct breeding: It is deliberate breeding of materials for stress resistance. When a genetically variable material is grown under the stress environment, selection can be based on survival, yield and other traits contributing to stress resistance.

1. Introduction

Anybody can become angry-that is easy, but to be angry with the right person and to the right degree and at the right time and for the right purpose, and in the right way-that is not within everybody’s power and is not easy.

– Aristotle.

The word emotion is derived from the French word, “emouvoir” which means ‘to excite’. Researchers have been interested in emotions since time immemorial. One of the earliest reported studies on emotions was attempted by Aristotle in 4th Century BC wherein he identified 14 distinct emotions. The traditional Indian art and literature talks about ‘navarasas’, nine emotions namely, joy (hasya), fear (bhayanaka), anger (raudra), love (shringar), courage (vira), sadness (karuna), amazement (adbhuta), disgust (vibhatsya) and calmness (shanti). Darwin (1872) theorized that emotions can be innate, evolved, and had a functional purpose. Robert Plutchik in 1980 talked about eight basic emotions, which he grouped into four pairs of polar opposites namely, joy-sadness, anger-fear, trust-distrust, surprise-anticipation. Renowned psychologist Paul Ekman and his colleagues through a cross cultural study in 1992 identified six basic emotions namely, anger, disgust, fear, happiness, sadness, and surprise. The popular animation movie produced by Pixar and released by Disney pictures in the year 2015,explored the emotional adjustment journey of a girl Riley, through the five basic emotions, anger, disgust, fear, happiness, and sadness, adapted from Ekman’s work. In a recent study by Cowen and Keltner (2017) researchers identified 27 unique emotions. In yet another study to identify emotions based on facial expressions irrespective of sociocultural influences by Jack et al. (2016) just four emotions, happiness, sadness, anger and fear were recognized.

Verbal communication-definition and meaning-Verbal vs Oral communication - Types-Styles-Barriers to effective verbal communication

Meaning

Verbal communication takes place directly between the superiors and juniors in organizations and between farmers and extension functionaries in the field and is often known as face to face communication. It takes the form of talks, a public address, verbal discussions, telephonic talks, telecommunications and other forms such as audio-visual aids speeches and orders, holdings of meetings and conferences, lectures, social get-togethers, training sessions, public address systems, museums, counseling etc.

Definition

The Verbal Communication is a type of oral communication wherein the message is transmitted through the spoken words. Here the sender gives words to his feelings, thoughts, ideas and opinions and expresses them in the form of speeches, discussions, presentations, and conversations.

Verbal vs Oral communication

Verbal Communication - Oral: It consists of spoken messages which include exchange of ideas, questions or comments. Few examples of verbal (oral) communication are face-to-face conversations, group discussions, counselling, interview, radio, television, calls, etc. Man is the only privileged species which can use language. Language is mostly used in speech and writing comes later. Oral communication has much wide and acceptable use when compared to written communication. As a part of everyday life, in informal relations or formal organization, we communicate more orally than in writing. Oral communication is a building block of human relations. Without oral communication, any interaction is lifeless. Thus, its importance cannot be ignored.

Verbal Communication - Written: This type of verbal communication does not require speech or sound. Though technically, written communication is nonverbal, it falls under this category as it includes the use of words and language. Few examples of verbal (written) communication are books, letters, text, newspapers, etc.

Definition-Importance of keeping a lab record-Features of a lab record - Contents of lab record-Guidelines for keeping a lab record

Definition

It is a primary record of research. Researchers use a lab note book to document their hypotheses, experiments and initial analysis or interpretation of these experiments.

Laboratory Record

The following is a general description of how to keep a proper laboratory notebook. Requirements for different teaching, research, clinical, or industrial labs will most likely vary. Some institutions/labs will require less stringent record keeping, others will hold you to a very strict protocol. A well kept notebook provides a reliable reference for writing up materials and methods and results for a study. It is a legally valid record that preserves your rights or those of an employer or academic investigator to your discoveries. A comprehensive notebook permits one to reproduce any part of a methodology completely and accurately.

Derivation & Meaning-Skills required-Method or procedure-Guidelines; Summarising-Meaning-Steps to write a summary.

Derivation

‘Precis’ is a French word which means to cut off, to be brief.

Meaning

A precise is a summary or the gist of the main ideas of written matter. Thus, precise writing means summarising. It is an exercise in concentration, comprehension and condensation. In order to make a summary of an article, a speech or a story, one has to read it carefully and grasp its meaning. Precise-writing forces one to concentrate on the material which is to be summarised. In summarising a passage, though the length of the precise is not fixed, it is generally expected that the it should be one third the length of the passage. The summary is known as precise and precise writing means summarising. A precise is not a paraphrase. At the same time, the essential points of the main passage must be presented in the precise in such a manner that reader may easily grasp the main ideas of the passage.

A better precise can be written with more and more practice. Before writing a precise, it is important that you take care of a few essential points.

Also, make sure that all the important points of the passage are conveyed in the precise. Focus on language and try and make it as clear, crisp and concise as possible. There is no fixed pattern to write a precise. But we must follow certain rules and procedure so that the precise can be more effective. Follow the right diction and you will be able to write a good precise.

Definition-Purpose of abstract-Types of abstract-Abstract Styles-Steps for Writing Effective Abstracts- Some Do’s Don’ts in preparing abstracts.

Definitions

The word abstract comes from the Latin word abstractum, which means a condensed form of a longer piece of writing. Abstracts are important parts of reports and research papers and sometimes academic assignments. The abstract is often the last item that you write, but the first thing people read when they want to have a quick overview of the whole paper. We suggest you leave writing the abstract to the end, because you will have a clearer picture of all your findings and conclusions.

An abstract is a paragraph that provides an overview of a paper. Abstracts should be between 100 and 200 words long and usually contain a list of keywords at the end to help readers identify the main points of the paper. While abstract requirements differ across academic disciplines, there are two main types of abstracts: humanities abstracts and scientific abstracts.

Purpose of abstracts?

Abstracts typically serve five main goals:

Male sterility refers to a condition in which pollen is either absent or non functional or unable to produce functionally viable pollen in flowering plants. Since it was first reported by Kolreuter as early in 1763 that anther abortion occurs naturally in some plant species or in their inter specific crosses, its occurrence was observed in several species. In the 19th century, Gartner (1844) and Herbert (1847) reported the occasional recurrence of anthers rudimentariness or abortion in many ornamental and vegetable crops in normal populations. During the early 20th century, a large number of species were shown to possess male sterility, for example, Suturela hortensis, Lathyrus odoratus, Merculalis annua, Solanum tuberosum, Vitis vinifera, Linnum usitatissimum, Zea mays, Antirrhinum majus, Prunus persicaer and Rubus idaeus. Following the rediscovery of Mendel’s Laws of Inheritance, study of the genetic main basis of inheritance of male sterility attracted the attention of several workers. The main impetus for the study of inheritance of male sterility came from the elucidation of heterosis in maize by G. H. Shull, in 1908 and its exploitation through the use of male sterility by Jones and Clark (1943). The male sterility based scheme of hybrid seed production in corn opened up a new era for the hybrid corn industry as it could overcome the necessity of detasseling of female (parent) lines in the hybrid seed production process.

The male sterility may arise due to malformation of male sex organs, lack of normal anther sac, and inability of anthers to release mature pollens, lack of development of normal microspore.

Mutagenesis is the process whereby sudden heritable changes occur in the genetic information of an organism not caused by genetic segregation or genetic recombination, but induced by chemical, physical or biological agents.

Mutations were first identified by Hugo de Vries in the late nineteenth century, while experimenting on the ‘rediscovery’ of Mendel’s laws of inheritance. He coined the term ‘mutation’. Radiation-induced mutations as a tool for generating novel genetic variability in plants, advanced as a field after the discovery of the mutagenic action of X-rays demonstrated in maize, barley and wheat by Stadler. The discovery of X-rays by Rontgen in 1895 led to the application of X-rays for inducing mutations in fruit fly by Muller (1927). Mutation is of two types

Advances in molecular biology have sharpened the tools of the breeders, and brighten the prospects of confidence to serve the humanity. The application of biotechnology to field crop has already led to the field testing of genetically modified crop plants. Genetically engineered Rice, Maize, Soybean, Cotton, Oilseeds like Rape seed, Sugar Beet and Alfalfa cultivars are expected to be commercialized in the late of 20^th century. Genes from varied organisms may be expected to boost the performance of crops especially with regard to their resistance to biotic and abiotic stresses

Genetic engineering involves isolation of DNA fragments within a cell recombining them outside. The basic technique is quite simple. Two DNA molecules are isolated and cut into fragments by one or more specialized enzymes, the fragments joined together in a desired combination, then restored to a cell from replication and reproduction. This method also referred to as recombinant DNA technology. Genetic engineering developed in the mid 1970 when it becomes possible to cut DNA and to transfer particular pieces of DNA containing specific bits of information, from one type of organism into other.

Biostatistics are the development and application of statistical methods to a wide range of topics in biology. It encompasses the design of biological experiments, the collection and analysis of data from those experiments and the interpretation of the results.

Quantitative traits are polygenic meaning they are controlled by many genes and gene interactions. Examples: height, weight, length etc.

Qualitative character

Qualitative traits are under the controlled of one or few genes, also called oligogeneic traits. For example, black or red coat color, horned or polled, coat color dilution, leaf color are all qualitative traits.

Importance of training and pruning

1. To control the habit of vegetative growth and regular bearing.
2. Remove all limbs and dead branches.
3. To improve flowering, fruiting and fruit quality
4. Remove all broken shoots, diseased, or pest infection branches from all trees and shrubs.
5. It is usually best to prune temperate fruits and shrubs during early spring before full leaf.
6. Maintain balance between shoots and roots at planting and nutrients uptake
7. Provide the basic tree form and to aid the development of a strong tree framework by encouraging strong wide crotch angles
8. Stimulate shoot growth near a cut.
9. Permit proper light distribution and penetration to the tree canopy.
10. Increase insect and disease control by allowing for better spray penetration.
11. Facilitate harvesting.
12. Achieve orchard uniformity.
13. Produce the proper amount of well distributed fruiting wood.
14. Encourage a structurally sound tree by removing branches with weak narrow crotch angles or those with diseased, damaged, or unproductive wood. Invigorate and renew the bearing area of older trees.

Introduction

Society is dynamic. The requirement of society varies in course of time. During the life process human being faces lots of health hazards which are mostly due to intake of various foods. So people become cautious about the intake of food and think about the quality of foods which can protect the health of human being. In ancient times people made medicines from plant extracts to protect their health. In 1989, Dr. Stephen coined the term “Nutraceuticals” which is combination of nutrition and pharmaceutical. Nutrition is required for health and pharmaceuticals provide remedy for sickness. So it is a preventive approach. Nowadays, the definition of nutraceuticals has been expanded to include vitamins, minerals, herbs and other botanicals, amino acids, and dietary substance for human use as a supplement diet (Stauffer, 1999).

Nutraceuticals are dietary supplements made only from whole foods to augment health. Currently over 470 nutraceutical and functional food products are available with documented health benefits (Brower, 1999). The patients are much aware about the side effects of chemical agents in short and long term therapy. So to avoid medicine used for small diseases, new therapies came into trend with the help of nutritional approaches i.e. “Nutraceuticals”. The use of nutraceuticals becomes easier than pharmaceuticals since nutraceuticals does not require any animal verification for its use in human. Nutraceuticals,

Functional foods and Dietary Supplements are most important concept in food science. They have distinction amongst themselves.

Introduction

Our traditional knowledge of medicine has been one of the strongest areas of research because of our rich heritage in herbal plants & great biodiversity in our fauna & flora. Consumption of plant foods and the structurally diverse natural products provided by them have long been associated with health-protective and therapeutic effects. Natural products in plant foods have been shown to have pleiotropic therapeutic and chemopreventive properties. These bioactive products are known to potentiate the efficacy of other drugs and have synergistic effects too. Most of the prescription drugs discovered so far is from plant sources and 75% drugs had been claimed in folklore claims and traditional medicinal system (Reynold, 1991; Benowitz, 1996). The traditional Indian medicinal system utilizes multicomponent formulations of plant extracts which may either act synergistically or avoid many side effects and might have added up trace element through combination of different plant extracts which could have additive effects. However the effort to utilize traditional knowledge in the light of modern scientific findings is lacking in India. Accumulating evidences as well as in-vitro studies implicates inverse relationship between regular consumption of fruits vegetable and the risk of specific cancer. Diet derived nutritional compound mediated interventions in the process of tumorigenesis may be promising chemopreventive strategy with minimal toxicities to the physiological system.

Introduction

During the first 50 years of the 20th century, scientific focus was on the identification of essential elements and their role in the prevention of various dietary deficiency diseases. This emphasis on nutrient deficiencies or “undernutrition” shifted dramatically, however, during the 1970s when diseases linked to excess and “overnutrition” became a major public health concern. “Let food be thy medicine and medicine be thy food”, the age old quote by Hippocrates is the ideology of today’s health conscious population. Eli Metchnikoff, the Russian Nobel prize winner was the first one to recognize the beneficial role of selected bacteria on gastrointestinal tract of humans. Subsequently the “Theory of Longevity” by Metchnikoff was correlated with prolonged youth and a healthy old age, observed largely in Balkan peasants of those times, who used cultured milks in their diet (Kaufmann, 2008). Since then, the quest continues for understanding the role of wide range of food components and nutrients in enhancing health or preventing chronic diseases. The research in this field has resulted in a plethora of new labels for pro-, pre- and synbiotics.

4.1 Introduction

Banana (Musa spp.) is one of the oldest fruits and second largest growing fruit crop in the world. It is also known as Adam’s Fig and Apple of Paradise. Important banana growing states are Maharashtra, Karnataka, Kerala, Tamil Nadu, Andhra Pradesh, Odisha, Bihar, West Bengal and Assam. In South India, banana is extensively used in all auspicious occasions such as wedding, festival and worship. Banana is a good table fruit. The cultivar Nendran is used for cooking; it is also used for preparation of halwa, sweet, chips etc. Main bud or heart of banana bunches is also used as vegetable in India, Thailand, Philippines, Malaysia and Indonesia. Musa textiles is known for strong fibre quality. Ripe banana contains about 20% sugar (but no starch) and about 4.7% protein. Further, it contains vitamins A, B, C, D and E and minerals such as K, P, Ca and Fe.

5.1 Introduction

Citrus is one of the most important fruit crops in the world. It is grown in the tropical and sub-tropical regions of the world. The top ten citrus producing countries are China, India, Nigeria, Brazil, Mexico, USA, Spain, Egypt, Italy and Argentina. The citrus industry in India is the third fruit industry after mango and banana. The important groups of crops in citrus are sweet orange, mandarins, limes, lemons, grape fruits and pummelo. The most important commercial Citrus species grown in India are mandarin (Citrus reticulata Blanco.), followed by sweet orange (Citrus sinensis (L.) Osbeck) and acid lime (Citrus aurantifolia Swingle). The major citrus producing states are Maharashtra, Karnataka, Andhra Pradesh and Punjab.

7.1 Introduction

Papaya is a fruit crop grown in kitchen garden, backyards of home and orchards. It is grown as a filler crop in orchard. India is the largest producer of papaya in the world. It is also cultivated in Brazil, Mexico, Australia, Hawaii, Malaysia, Taiwan, Peru, Florida, Gold Coast, South Africa and Bangladesh. In India, it is widely cultivated in Uttar Pradesh, Bihar, Karnataka, Jharkhand and Madhya Pradesh. The ripe fruits of papaya are consumed throughout the tropics and sub-tropics. Fruits are also used in the preparation of jam, soft drinks, ice cream flavour, crystallized fruits and syrups. Unripe fruits are commonly used as vegetables. Papain is prepared from the latex of immature fruits. It is a proteolytic enzyme used for tendering of meat, preparation of chewing gum and cosmetics. The ripe fresh fruit is rich source of vitamin A, carbohydrates and minerals. The fruits are prescribed for piles, dyspepsia, liver, spleen and digestive disorders. Roots are used as nerve tonic. The plants flower five months after planting and fruits are ready five months after flowering. So, it has attracted attention of fruit growers to cultivate on a large scale in the country.

8.1 Introduction

Guava is the fourth important fruit crop next to mango, banana and citrus. It is known as the ‘Apple of tropics’. It is very rich and cheap source of Vitamin C and also contains a fair amount of calcium. Important guava growing states are Uttar Pradesh, Bihar, Madhya Pradesh and Maharashtra. Allahabad district of Uttar Pradesh has the reputation of growing the best quality of guava fruits in the world. The importance of guava is due to the fact that it is the hardy fruit which can be grown in alkaline and poorly drained soil. Guava fruits are used for making jam, jelly and various culinary purposes. The fruit can be canned in sugar syrup.

Any commercial plant breeding programme run by the private seed companies typically called as Research and Development (R & D) is carried out through various models operating. There is no fixed organizational set up and different companies adopt the working set up as per size of their programme, availability of manpower and resources, area of the breeding station and several other considerations. One thing is clear that any commercial plant breeding programme will have more numbers of crop breeders than the supporting scientists.

A significant development in the field of agricultural research in India is the formulation of All India Coordinated Research Projects (AICRPs), initially for the improvement of agricultural crops, but later extended to all aspects of crop husbandry.

Diagnostic Symptoms

• At first pale-green chlorosis, typically appear on lower leaves and extending up the plant.Leaves eventually take on a dull-yellow colour, wilt and the plant collapses and dies.

Powdery Mildew

Erysiphe polygoni

Diagnostic Symptoms

• At first symptom appears on leaves.

• White floury patches appear on both surface of leaves as well as tendrils, stem and pod.

Anthracnose or Fruit Rot and Die Back

Colletotrichum capsici

Diagnostic Symptoms

Dieback symptoms

• Small, circular to irregular, brownish black scattered spots appear on leaves .

Powdery mildew

Sphaerotheca pannosa var. rosae (Sphaerotheca pannosa)

Diagnostic Symptoms

• A white, powdery fungal growth on the upper, lower or both leaves and shoots.

MEANING & DEFINITION OF ACCOUNTING

Accounting is the science of recording, classifying and summarizing business transactions and events primarily of a financial character in a significant manner and interpreting and communicating the results to decision-making persons thereof.

The AICPA defined, Accounting is the process that are used in accounting, recording, classifying, summarizing and interpreting the monetary language in which financial language is expressed.

ABSTRACT

With the advancement of mobile technology, the usage of mobile application has increased significantly which leads to change the life style of common people. Library is also not an exception in this context. Library and Information Science Professionals have started to incorporate the changes from the beginning of ICT advancement with an idea “library should be within thumb of people”. Mobile application plays vital role to fulfil the objective of library. Most of the library has started or planning to start the service to its user with various services effectively. But, the process to provide library service through mobile application is not satisfactory till date. No too much usage of libraries mobile apps can be seen; even which mobile applications are in use, most of them are almost inactive form.

But the various recent surveys show us the tremendous growth of the usage of mobile application among people. According to Google, (2017) the user survey shows that mobile can fast-track time to purchase by 20% through facilitating efficiencies in decision-making and enhanced team collaboration, particularly with more complex purchases.

ABSTRACT

The chapter focuses that information as an economic resources and the role of library to distributing information among the user’s communities. From the economic point of view-the most important properties of information are easy to access and limited money acquire more information i.e. cost benefit. Information economy makes people ware about their goal and library help people to provide resources on need base.

Keyword: Information economy, Library, Information Communication Technology, ICT, Users’ community.

ABSTRACT

Academic libraries are unique, essential and strategic hubs inside our social cloth which are advanced primarily based totally at the requirements of the times, to help people in the purchase of good enough facts that could allow them to decorate their information, for the development in their wellness and that of the society at large. To attain their goal, they ought to generate, filter, control and diffuse correct and well timed human information which have been harvested and documented; for the purpose of enriching the highbrow needs in their clients. Like every other kind of library, academic libraries are growing organisms. Justification to the above statement has been captured aptly in the classic book of Ranganathan (1931) entitled ‘Five Laws of Library Science’; detailing the principles of the library profession. The philosophical postulation of Ranganathan’s fifth law is a classical position that offers much strength and support for the need to adequately plan for the best strategies to exhibit in academic library environments in order to provide effective services. In other words, the fore-stated law by Ranganathan has already signaled that academic librarians must be ready at all times to effectively satisfy the diverse, complex and ever-increasing information needs of their users from the explosive available information resources that spring on a daily basis. As such, addressing this issue would require a holistic management approach by academic libraries that must be internally efficient and externally effective.

ABSTRACT

It is very important for every library to have all the resources/materials necessary to fulfil its target. The working of a library can be classified into two forms: dealing with the users and behind the scene. There are numerous different sections in a library divided on the basis of their functions. Most important of these is the Acquisition section. A library’s success lies in a well organized acquisition section. Main aim of the library is the contentment of the users. However, collection development is the primary task of the acquisition section which further contributes to user satisfaction itself. It performs the process of selection, procurement and accessioning of reading materials as well as maintains records. It organizes the monetary as well as human resources in such a manner that collection is facilitated more and that too in an economic and less time consuming way. All this is the responsibility of the librarian. Before acquiring the reading materials, the librarian is supposed to decide that which items are actually required in the library and which are to be rejected. A librarian must study the requirements of the library and acquire materials as per the priorities of the institution to achieve its goal.

3.1. Types of communication network

There are different situations in which different kind of communication is required for transfer of technology. An Extension expert works in an organization.

4.1 The non-verbal communication

The non-verbal communication is the use of human body as the medium of message. The non-verbal communication forms a vital part of the process of communication that an individual goes through daily.

A pH meter is very commonly used for the measurement of the reaction of a solution or a medium. The pH determination with the help of a pH meter is based on the measurement of the electromotive force (e.m.f) of a pH cell consisting of an electrode sensitive to W ions and a reference electrode.

The term biodiversity is used by Walter and Roses in 1985. Biodiversity means the existence of a large number of different kinds of animals and plants which make a balanced environment. According to “Convocation on biological diversity (1992)”-Biodiversity is present in terrestrial, ocean and aquatic ecosystem. This diversity present in between same species, different species and in different ecosystem.

Ultrasonographic examination of gastrointestinal tract is not very much rewarding in dogs because of gas in the tract but it is crucial for a correct diagnosis. Imaging of stomach plays a significant role in the diagnosis of gastric diseases such as gastric dilatation, gastric foreign bodies, pyloric outflow obstruction, or gastric tumours.

Ultrasound and survey radiography are two important diagnostic modalities regularly employed in the diagnosis of intestinal diseases. Both modalities are complementary to each other in investigating the problems related to intestines. It is always better to have plain survey radiograph of abdomen first and then ultrasound to rule out obstruction or radio-opaque foreign bodies. Wherever barium meal radiograph is indicated, it should be done after sonographic examination as barium may degrade image quality of the sonogram. Assessment of intestinal motility can be done in real time sonography.

Architecture of the organ is affected in the primary or secondary diseases of the organs. Ultrasonography plays a significant role in detecting the changes in the organs in various diseases.

During last two decades significant advances have been made in diagnostic ultrasound .These advances have positively impacted on diagnostic imaging across all species of animals and have made ultrasound as an indispensable diagnostic modality in veterinary medicine. Since equines have been more a sport and pack animals, the ultrasound technique has long been utilized in the diagnosis of equine musculo-skeletal disorders. Recent years have witnessed its utility in the management of equine reproduction beside its use in the diagnosis of colic and other diseases.

Biometrical genetics: This is a branches of genetics which uses the statistical concept and procedures for the study of genetic principles.

There are various methods available for isolation of high molecular weight genomic DNA from plants. The basic steps involved in all these methods are removal of cell wall, nuclear membrane, proteins, lipids, carbohydrates, cell debris, RNA without affecting the integrity of DNA. The two major factors that affect DNA integrity are action of endogenous nucleases and impact of mechanical shearing.

Polysomes consist of two or more ribosome traversing a strand of mRNA, translating the nucleotide sequence into the corresponding amino acid sequence. Usually at any time, many ribosomes are translating an mRNA simultaneously forming a structure called a polyribosome or polysomes. One reason for isolating polyribosomes is to resolve them on sucrose gradients to determine the extent of ribosome loading. The second main reason is to provide a source of mRNA for in vitro translation.

Exchange of genetic material rarely occurs between unreleated organisms and even similar species may not exchange chromosomal genes. A certain degree of DNA sequence homology is a prerequisite for genetic recombination in vivo. However, recombination between DNA molecules in vitro is not subject to such taxonomic restrictions.

Recombinant DNA is advancement in molecular technology which allows manipulation of genomes of higher organisms and dissection of their complex regulatory circuits. This new methodology is being used to understand the biochemistry of living organisms. The isolation of gene begins with the cloning of fragmented DNA of the organism into appropriate vector.