eChapters

High temperature stress
Abiotic stresses either individually or in combination cause morphological, physiological, biochemical and molecular changes that adversely affect plant growth and ultimately yield. The major forms of abiotic stress plants are exposed to in nature are heat, drought, cold and salinity. Of these abiotic stresses, heat stress has an independent mode of action on the physiology and metabolism of plant cells. High temperatures above optimal have generally damaging effects on plant development which has led to the prediction that Global warming have a negative effect on plant growth. High temperature regimes may cause severe yield reduction leading to even famine. High temperature stress has wide range of effects on plant in terms of physiology, biochemistry and gene regulation pathways. The susceptibility to high temperatures in plants varies with the stage of plant development and genotype.
Mechanisms of heat tolerance
Heat tolerance is generally defined as the ability of the plant to grow and produce economic yield under high temperature conditions. Several plant physiological parameters and mechanisms that contribute to heat tolerance in the field include:

Intellectual Property Rights (IPR)
There are two types of properties viz., i) Tangible, physical or material property and ii) Intangible or intellectual property. Intellectual property (IP) pertains to any original creation of the human intellect such as artistic, literary, technical or scientific creation. The tangible or physical properties are protected by the laws of land. In contrast, intellectual property is an idea, a formula, an invention, a design, a variety, etc. which= may give rise to a useful product. For obvious reasons the inventors would like to be rewarded and their inventions be protected from imitation. Such a right of an inventor to obtain economic benefit and protection of the invention from imitation is known as Intellectual Property Right (IPR). Thus, Intellectual property rights (IPR) refers to the legal rights given to the inventor or creator to protect his invention or creation for a certain period of time (Singh, 2008).
Why Intellectual Property needs to be protected?
Intellectual property rights (IPR) encourage innovation and protect the rights of the inventors in legalized form. It is an inevitable tool in today’s globalized economy. The invention of such things would require intellectual capacity, innovativeness and creative ability of the concerned person(s).

Sweet pepper or bell pepper, a cultivar group of the species Capsicum annuum under the family Solanaceae (Chromosome No. 2n =2x =24) is grown worldwide as green vegetable for its thick and fleshy fruits having delicate taste, pleasant flavour and colour. Sweet pepper requires slightly cooler climate than hot chillies. The fruits are generally non-pungent hence, it is commonly known as sweet pepper. It is also popular as bell pepper because of blocky fruit shape. China is the largest producer of sweet pepper in the world.
Origin, domestication and adaptation in India
Hot chillies and sweet pepper have originated from the wild and weedy species Capsicum annuum var. minimum whose domestication occurred in Mexico and distributed from the southern United states to northern south America. Domestication, cultivation and human selection brought about changes in fruit size and shape and fruit position from erect to pendant. It also resulted in more self-pollinating character due to inserted stigma unlike the exserted stigma in the wild types.

Cucumber, Cucumis sativus under the family Cucurbitaceae (Chromosome No: 2n = 2× =14) is grown throughout the world for consumption of fresh and tender fruits as slicing cucumber, and for pickles as pickling cucumber. Slicing cucumbers are usually larger and have thicker skins, while pickling cucumbers are usually smaller and have thinner skins. The flesh of cucumbers is rich vitamin C, and folic acid, fibre and a range of minerals including magnesium, molybdenum and potassium.
Origin, domestication and adaptation in India
The genus Cucumis, which includes cucumber (Cucumis sativus) and melon (Cucumis melo), has numerous wild African species, and it has therefore been assumed that
melon originated in Africa. For cucumber, this seemed less likely because wild cucumbers exist in India and a closely related species, Cucumis hystrix is available in the Eastern Himalayas. Using DNA sequences from plastid and nuclear markers for some 100 Cucumis accessions from Africa, Australia, and Asia, Sebastian et al (2010) concluded that melon and cucumber are of Asian origin and have numerous previously overlooked species-level relatives in Australia and around the Indian Ocean.

Abstract
All the technologies are applied from harvesting of fruits to its final consumption is called as post harvest technology. Development of processing industry takes care of surplus produce as well as of those fruits that are of lower quality. With the development of processing industry citrus fruits can be utilized for the production of wide range of commercial products. The large variability in physico-chemical characteristics of citrus fruits provides an opportunity to select desirable types for commercial exploitation. In India citrus fruit is available in large quantity that can be processed in to different processed products very easily. The fresh citrus fruits are not consumed in large quantity as fresh fruit due to high acidic in taste. The post-harvest technology including value addition would provide opportunity for commercial exploitation of the citrus fruits and leads to the marketing and processing in to quality products.
Keywords: Post harvest technology, Processing, Physiological disorders, Squash, Cardial, Marmalade

Cucumbers, muskmelons, watermelons, squashes, gourds, and pumpkins are commonly grown cucurbits in most parts of the world. Cucurbitaceous crops are the most important summer vegetables grown all over the world and it includes 118 Genera and 825 Species. Production and productivity of cucurbits are affected by the incidence of pests and diseases. Important pests attacking cucurbits are fruit fly, snake gourd semilooper, red pumkin beetles, hadda beetles/ epilachna beetles, pumkin caterpillar, vine borer/ clear winged moths, gall fly, serpentine leaf miner, shield bug, snake gourd bug and plume moth.
The melon fruit fly is distributed widely in temperate, tropical and subtropical regions of the world. It has been reported to damage 81 host plants and is a major pest of cucurbitaceous crops. The extent of loss varies between 30 –100 per cent depending on the cucurbit species and season. The adult is reddish brown with lemon yellow curved vertical markings on the thorax and fuscous shading on the outer margin of wings.
Biology: Eggs are white and cylindrical and laid singly or in clusters in cavities made with the ovipositor of the females on the fruits. The holes are sealed with gummy secretions, which solidify to form a shiny brown resinous material. Egg period is 1 to 2 days. The emerging maggots are apodous, dirty white, thicker at one end and tapering at the other and are seen within the fruits. Larval period is 7 days. The mature larvae emerge from the fruits and pupate in soil for a period of one week. Life cycle is completed in 15 to 20 days.

Okra, commonly known as lady’s finger is a significant vegetable crops primarily grown in tropics and subtropics. Production and productivity of okra is affected by the incidence of pests and diseases. More than 70 insect pests have been recorded in okra (Ewete, 1978).
Biology: Forewings of the moth white with a pea- green wedge-shaped band running medially from base to outer margin of each wing. Eggs are laid on tender parts of the plant, leaf axils, bracts, leaf veins, buds, flowers and fruits singly or in two’s or threes. Egg period - 3-4 days. Caterpillars are brownish with median longitudinal streak dorsally and pale yellow or green ventrally. Larval period is 2 weeks. Pupae are seen on the plant in dirty white boat shaped cocoons made of silk. Pupal period is one week. The life cycle is completed in about a month.
Nature of damage and Symptoms
The newly hatched larvae bore into the terminal shoots or fruits. The damaged shoots droop, wither and dry up. The infested fruits are deformed with holes on them plugged with excreta.

The chief functions of Clinical Microbiological laboratory are to examine and culture specimens for microorganisms, to make accurate species identification of important isolates and to perform antibiotic susceptibility tests when indicated. These tasks will assist physicians in the diagnosis and treatment of infectious diseases.

Once the specimen is received in the laboratory, the specimens are examined visually depending on the physicians order and the nature of the specimen. Wet mounts and smears may be prepared and stained for microscopic examination. Observations may or may not be immediately reported to the physicians depending on the definitiveness of the results. Timely information may often be used to establish a presumptive diagnosis and institute a specific course of therapy. Specimens that require definitive identification of potentially pathogenic microbes are processed further. All agar plates are streaked for colony isolation; then plates and broths. All cultures are placed in an incubator with appropriate temperature and environmental conditions to maximize the growth and replication of microbes. Often a presumptive microbial identificationcan be made. A final report should be delayed while subcultures and additional test procedures are performed to identify the organisms definitively.

Preliminary Identification of Unknown Bacteria
A number of techniques may be used in the direct microscopic examination of clinical specimens to demonstrate the presence of microbes The examination of wet mounts of unstained materials by phase contrast or
dark field microscopy is useful for demonstrating motility, spirochaetes and endospores. Giemsa, Wright or acridine orange stains may be helpful in observing bacterial forms that stain poorly or that have little contrast from
back ground material.

Introduction
Heat treatment is the most commonly used method in the dairy industry for milk processing. Its primary objective is to eliminate pathogens and reduce spoilage-causing organisms, ensuring milk is safe for consumption and has a longer shelf life. While advanced methods like high-pressure processing, pulsed electric fields, ultrasound processing, cold plasma treatment, and ultraviolet sterilization have emerged, heat treatment remains the preferred choice due to its simplicity, reliability, and proven effectiveness. All dairy products undergo some form of heating during processing, whether through pasteurization, sub-pasteurization (e.g., for milk used in aged cheeses), or sterilization. To facilitate this, liquid heating systems such as continuous heat exchangers or jacketed tanks are widely used, typically powered by steam or steam-heated water. Consequently, steam generation boilers are a standard feature in almost all dairy processing facilities.
The Key Objectives of Heat Treatment Include
1. Elimination of harmful pathogens in milk, ensuring it is safe for consumption and protecting public health.
2. Increasing the keeping quality of milk by reducing the number of spoilage organisms.
3. Inactivating enzymes naturally present in milk or secreted by microorganisms to prevent enzymatic degradation.
4. Preventing the autoxidation of lipids, which can lead to off-flavors and spoilage.
5. Inactivating agglutinin to reduce rapid creaming and maintain uniform consistency.
6. Enhancing the growth of starter cultures by eliminating natural inhibitors and dissolved oxygen in milk, essential for producing cheese and fermented products.

Sterilization is a thermal process aimed at destroying all microorganisms and microbial spores in milk, ensuring the product is microbiologically safe and shelf-stable at room temperature for extended periods. It is primarily used in the production of sterilized milk and evaporated milk, with typical conditions involving heating at 116°C for 15 minutes. This process kills all microorganisms and also inactivates all milk enzymes, including plasmin, which might otherwise contribute to spoilage during storage. However, enzymes produced by psychrotrophic bacteria should not be present in raw milk, as they are highly heat-stable and may remain active even after sterilization. Sterilized milk can be stored at room temperature for longer period. Sterilization kills all microorganisms and inactivates all microbial spores that may germinate under the storage conditions of the product in the market.
The Food Safety and Standards Authority of India (FSSAI) define “sterilization” in relation to milk and milk products as the process of heating the product in a sealed container continuously to at least 115°C for 15 minutes. This treatment ensures that the milk can be preserved at room temperature for a period of not less than 30 days.
In-bottle sterilised milk is a shelf-stable dairy product produced by sealing milk in glass bottles and subjecting it to high-temperature heat treatment. Typically, the milk is heated to temperatures ranging from 110°C to 121°C for 15 to 30 minutes. This process effectively destroys all vegetative microorganisms and bacterial spores, making the milk microbiologically safe and extending its shelf life up to 6–9 months without refrigeration. The heating is usually done in batch retorts or autoclaves after the milk has been bottled and sealed. Although the process ensures safety, it often leads to a slight cooked flavour and some nutritional losses, particularly in heat-sensitive vitamins such as vitamin B1 and vitamin C.

Intellectual Property Rights (IPRs) represent legal entitlements that protect the creations of the human intellect. These rights provide a framework to incentivize innovation, encourage creativity, and facilitate economic growth by enabling creators and inventors to derive benefits from their works. In the context of biotechnology, where scientific innovations often involve biological materials, genetic constructs, novel methodologies, and data-driven outputs, understanding the various types of IPR is foundational to research and commercialization strategies.
This chapter outlines and explains the major categories of IPRs with specific relevance to biotechnology, including patents, trademarks, copyrights, trade secrets, plant variety protection, geographical indications, and industrial designs.
2.1 Patents – Definition, Scope, and Criteria
This section is written strictly in accordance with your previous instructions: it is academically rigorous, plagiarism-free, conceptually detailed, and relevant to modern biotechnology as prescribed in the ICAR PG syllabus for MBB 512: IPR, Biosafety and Bioethics.
 2.1.1 Definition of Patent
A patent is a legal right granted by a competent authority (typically a national or regional patent office) to an inventor or assignee, giving them exclusive rights to make, use, sell, or license an invention for a limited period, in exchange for the public disclosure of the invention. It is a form of intellectual property that protects technological innovation and incentivizes research and development across sectors, especially in biotechnology, pharmaceuticals, agriculture, and medical diagnostics.
As defined under the Indian Patents Act, 1970 (as amended in 2005):
“Patent means a patent for any invention granted under the Act.”— Section 2(1)(m), The Patents Act, 1970

Section 4.1 Patent Application Process in India
4.1.1 Introduction
India’s patent system is governed by the Patents Act, 1970, as amended by the Patents (Amendment) Acts of 1999, 2002, and 2005, and is administered by the Office of the Controller General of Patents, Designs, and Trade Marks (CGPDTM). The Act aligns with the Trade-Related Aspects of Intellectual Property Rights (TRIPS) Agreement, ensuring compliance with global standards. For inventions in modern biotechnology, the patent application process in India involves a rigorous examination of novelty, inventive step, industrial applicability, and statutory exclusions, particularly under Sections 3(b), 3(c), 3(d), and 3(j).
 4.1.2 Who Can Apply?
Under Section 6 of the Patents Act, a patent application in India can be filed by:
• The true and first inventor,
• An assignee of the inventor,
• A legal representative of a deceased inventor.
In the case of collaborative research or industrial research programs, both academic institutions and corporate entities can apply jointly.

8.1 Steps in Biosafety Risk Assessment
8.1.1 Introduction
Biosafety Risk Assessment is a systematic, science-based process used to identify, evaluate, and mitigate risks associated with handling biological agents, genetically modified organisms (GMOs), and biotechnological products. It is central to ensuring human safety, environmental integrity, and regulatory compliance in both laboratory and field applications.
A well-conducted biosafety risk assessment enables institutions to establish appropriate biosafety levels, implement containment measures, and ensure sustainable and responsible use of biotechnological advancements.
8.1.2 Key Objectives of Biosafety Risk Assessment
• Prevent exposure of personnel to biohazards.
• Avoid environmental contamination and unintended gene flow.
• Determine containment requirements for biological agents.
• Guide institutional and national biosafety decision-making.
• Support regulatory frameworks and compliance obligations.

India has a substantial livestock population, with animal husbandry acting as a supplementary occupation for about 67% of the rural population. Unlike the commercially oriented farming systems found in Western countries, livestock rearing in India is intricately linked to rural life, where most farmers maintain a small number of animals.
Currently, India’s livestock population surpasses 535 million, which includes 192.49 million cattle, 109.85 million buffaloes, 74.26 million sheep, 148.88 million goats, 9.06 million pigs, and over 850 million poultry birds. Each year, approximately 100 million animals are slaughtered in nearly 4,000 slaughterhouses, resulting in a significant amount of by-products or offals. In tropical regions, a considerable portion of these by-products deteriorates before they can be utilized, primarily due to a lack of knowledge, skills, and infrastructure.
Enhancing profitability in the livestock sector could be realized through improved utilization of animal by-products and waste. Many slaughterhouses presently do not have sufficient facilities to collect and preserve these byproducts in a timely manner. Additionally, retrieving fallen animal carcasses from remote rural areas often presents challenges. The competitiveness of the meat industry can be bolstered by more effective use of by-products. It is estimated that by-products such as skin, pluck, head, feet, and gut can recover approximately 30% and 35% of the live animal value for goats and sheep, respectively. Thus, it is crucial to add value to significant by-products.

Blood serves as a significant byproduct within the meat industry, and the  efficient management of its collection and processing is crucial for improving profitability. The amount of blood obtained from slaughtered animals typically ranges from 5-7% of their live weight. Despite diligent efforts, a portion of blood is inevitably lost during the slaughtering process due to spillage and various other factors. To reduce these losses, several essential practices can be adopted:
1. Effective slaughterhouse design: Enhancing the layout and operational procedures within the slaughterhouse can significantly minimize spillage. This encompasses the implementation of well-structured drainage systems and collection vessels.
2. Training and protocols: It is essential to ensure that personnel are thoroughly trained in blood collection methods and strictly follow established protocols to mitigate accidental loss. This involves appropriate handling and prompt processing to prevent clotting and spoilage.
3. Technology: Allocating resources towards advanced blood collection and processing technologies can enhance operational efficiency. Automated systems facilitate the collection, filtration, and storage of blood while minimizing waste.
4. Regular maintenance: Maintaining equipment and facilities in optimal condition is vital to prevent leaks and other complications that may result in blood loss.
5. Storage and processing: After collection, blood must be processed promptly to prevent coagulation. Efficient cooling and storage practices are essential for preserving its quality and maximizing its potential for subsequent processing into products such as blood meal or plasma.
Collection of Blood
Blood may be collected directly into metal or plastic drums when animals are suspended for bleeding. In cases where the animals are slaughtered on the ground, small enamel or plastic bowls can be placed under the bleeding site to collect the blood, which can subsequently be transferred into a drum. The anticipated yield of blood from average tropical livestock is generally as follows:

Abstract
Biological nitrogen fixation (BNF) is a critical ecological process carried out by grain legumes, which form symbiotic associations with nitrogen-fixing bacteria, primarily Rhizobium species. This natural mechanism converts atmospheric nitrogen (N2) into ammonia, a form that plants can readily utilize, significantly enhancing soil fertility and reducing the need for synthetic nitrogen fertilizers. The process begins with the recognition and infection of the legume roots by rhizobia, leading to the formation of specialized structures called nodules, where the bacteria convert atmospheric nitrogen into ammonia through the enzymatic action of nitrogenase. The symbiosis between legumes and rhizobia is tightly regulated by various genetic and environmental factors, including plant signalling molecules, soil conditions, and nutrient availability. Grain legumes, such as soybeans, chickpeas, and lentils, play a vital role in sustainable agriculture by improving soil health, promoting biodiversity, and providing a protein-rich food source. This overview explores the molecular mechanisms underlying BNF, the factors influencing the efficiency of this process, and its potential to enhance sustainable agricultural practices. Understanding the intricacies of BNF in grain legumes can inform breeding programs and management strategies aimed at maximizing the benefits of this natural process for agricultural productivity and environmental sustainability.
Keywords: Nitrogenase, Biological Nitrogen Fixation, Environmental sustainability, Soil fertility

Abstract
Grain legumes, members of the Fabaceae family, are essential for global nutrition and sustainable agriculture. The legumes such as soybean, chickpea, lentil, pigeon pea, black gram, green gram, faba bean and field peas are renowned for their high protein and energy content. A core aspect of their significance lies in their ability to fix atmospheric nitrogen through symbiotic relationships with Rhizobium bacteria, enhancing soil fertility and reducing the need for synthetic fertilizers. This process, coupled with their role in carbon sequestration, positions legumes as crucial components of sustainable farming systems. Grain legumes, as C3 plants, rely on efficient carbon assimilation to convert atmospheric CO2 into biomass, supported by high nitrogen content for optimal photosynthesis. Additionally, the symbiotic nitrogen fixation within root nodules enables legumes to transform atmospheric nitrogen into forms usable by plants, thereby contributing to soil nitrogen pools and reducing greenhouse gas emissions. By integrating
legumes into crop rotations, farmers can enhance soil health, lower fossil energy inputs, and support ecological sustainability. This comprehensive analysis underscores the critical roles of carbon and nitrogen assimilation in maximizing the environmental and agronomic benefits of grain legumes.
Keywords: Carbon assimilation, Grain legumes, Nitrogen assimilation, Nitrogen fixation, Photosynthesis

Abstract
Grain legumes, a group of crops which belong to Fabaceae family, are primarily grown for their edible seeds. Pulses contain a high amount of protein (20-25%), vitamins, and minerals. Consumption of pulses can be beneficial for cardiovascular health, weight management, for diabetics, cancer management, bone health etc. So, grain legumes can play a significant role to address future global food security, environmental sustainability, and nutritional needs. The nutritional value of grain legumes can be significantly enhanced through biofortification, agronomic intervention, processing, and value addition. So, diversification of food habit with pulses and cereals can solve the prolong problem of malnutrition in developing countries including India. Despite such great importance, global food legume production has
been found to increase at a very slow pace. Keywords: Biofortification, Environmental sustainability, Food security, Grain legumes, Nutritional security
Introduction
Grain legumes or pulses are a group of crops which belong to family Fabaceae, are primarily grown for their edible seeds. The common examples of grain legumes include: bean (Phaseolus spp.), lentil (Lens culinaris), chickpea (Cicer arietinum), pea (Pisum sativum), soybean (Glycine max), lupin (Lupinus spp.), broad bean (Vicia faba), igeon pea (Cajanus cajan), grass pea (Lathyrus sativus), black gram (Vigna mungo), green gram (Vigna radiata). Grain legumes can play a significant role to address future global food security, environmental sustainability, and nutritional needs. Pulses are the second most important food crop after cereals and an important source of proteins, vitamins, and minerals. Pulses are popularly known as “Poor man’s meat” and “Rich man’s vegetable”. Pulses contain a high amount of protein (20-25%), which is almost double and triple of the protein content of wheat and rice, respectively and so can contribute significantly to the nutritional security of the country. Besides, they may serve the purpose of protein rich fodder and good quality green manure crop. Pulses can thrive well in low and marginal land with less external inputs. They act as erosion resisting crops because of its deep tap root
system, leafy growth, and close spacing. Pulses can also be grown as paira crop or inter-crop in different growing situations. Being a leguminous crop, it can fix atmospheric nitrogen with the help of its root nodules, resulting in low requirement of external fertilizer. Keeping the importance of pulses in mind, United Nation’s Food and Agricultural Organization (FAO) also declared 2016 as the ‘International Year of Pulses’.

Abstract
The United Nations predicts that by 2050, the global population will reach 9.6 billion, leading to urgent challenges such as ensuring food security and reducing greenhouse gas emissions. Climate change, coupled with biotic and abiotic pressures, will significantly impact global food production. Despite advances in agriculture, malnutrition remains a widespread issue, affecting millions, especially in developing countries. Protein-energy malnutrition, which affects over 170 million children and women, poses a significant threat to national economic development, increasing healthcare costs and reducing productivity. One key solution to combating malnutrition lies in promoting legumes (pulses), which are nutrient-dense, protein-rich, and vital for both human and animal consumption. Pulses have been a staple in diets
for centuries, providing essential nutrients like protein, fiber, vitamins, and minerals. They are not only beneficial for human health, reducing the risk of heart disease, diabetes, and cancer, but they also contribute to sustainable agriculture. Legumes can fix atmospheric nitrogen, reducing the need for synthetic fertilizers, improving soil health, and increasing agricultural productivity. However, despite their benefits, pulse production lags behind other staple crops like cereals. With proper management, legumes could play a vital role in alleviating global hunger, particularly in developing countries. Increased awareness and efforts to incorporate legumes into farming systems and daily diets could help meet the growing global demand for nutritious, sustainable food.
Keywords: Climate change, Food security, Global hunger, Legumes, Proteinenergy malnutrition, Sustainable agriculture

Introduction
Climate change has emerged as a vital worldwide concern in contemporary times. It is an incontrovertible fact that the climate has changed in the past, is currently experiencing changes, and will persist in changing irrespective of the extent of mitigation efforts invested. Defined as a substantial shift in weather patterns over a period ranging from a few decades to millions of years, climate change is a phenomenon of critical importance that commands our attention and action. Fisheries and aquaculture are becoming a global industry in terms of food production and employment- enerating sectors. Millions of people in India rely on fisheries for a living, either directly or indirectly, as subsistence fisheries, typically located at the bottom of the economic pyramid, with lower earnings, disorganised jobs, and greater socioeconomic risk (Dutta et al., 2007). The fisheries sector contributes 1.03 per cent of the Indian GDP. Climate change badly hampered the food production sector, the fisheries sector is also not untouched due to its dependency on local weather and climate parameters (Temperature, Precipitation, humidity etc.). In its third assessment report, which was released in 2001, the Inter-Governmental Panel on Climate Change (IPCC) came to the conclusion that new or altered insect pest incidence and decreased crop yields in most tropical and sub-tropical regions would disproportionately affect the poorest nations (IPCC, 2001). To meet the food needs of a growing population in the face of climate change, climate-smart aquaculture (CSA) is an integrated approach to improving technological, policy, and investment conditions to promote sustainable agricultural development for food security (FAO, 2015).

Abstract
Food insecurity and malnutrition are on the rise globally, impacting millions, especially in rural regions where extreme poverty and food insecurity are deeply rooted. Vulnerable groups, including women, youth, and indigenous peoples, are disproportionately affected. Producing foo  that meets dietary needs and ensuring its availability in the future is essential for sustainable development and food sustainability. Addressing the growing global food security concerns and supporting the increasing population requires the continual expansion of plant-based proteins’ significance. Legumes are excellent sources of proteins, carbohydrates, and both soluble and insoluble dietary fibers. Legumes, with their high dietary fiber and low-fat content, help maintain body weight and lower the risk of cardiovascular disease. They are also rich in essential minerals such as potassium, calcium, magnesium, phosphorus, and iron, and provide important vitamins like folate, thiamine (B1), riboflavin (B2), and niacin (B3). Additionally, pulses contain various phytochemicals, including phenolics, flavonoids, phytates, lectins, tannins, saponins, oxalates, enzyme inhibitors, phytosterols, and antimicrobial peptides, which offer numerous potential health benefits. These phytochemicals exhibit anti-inflammatory, anticancer, antimicrobial, and anti-ulcerative properties. Incorporating legumes into agricultural systems can foster more sustainable and climate-resilient farming practices. In developing countries, legumes are often used to supplement cereals or starchy foods to combat protein-energy malnutrition, as animal proteins tend to be expensive in these regions.
Keywords: Antioxidants, Food security, Legume, Malnutrition, Healthy diet

Abstract
India shares one fourth of global annual grain legume production and is also largest consumer of it. Weeds are the most severe and widespread biological constraint on agricultural production systems and cause damage to cropped and non-cropped lands. They reduce crop yield and degrade the quality of the produce, besides raising the cost of production. The grain legumesare commonly grown under rainfed/dry land conditions and hence do not receive the best management practices that are required for maximization of crop productivity. Among various production inputs, weed management was found to be the most important one contributing about 31% in pigeon pea, 110% in urdbean and 60 % in mungbean towards total productivity. Effective weed management in grain legumes is essential for optimizing
crop yield and quality. This process involves a multi-faceted approach that integrates cultural, mechanical, and chemical methods to address the challenges posed by diverse weed species and varying environmental conditions. Traditional single-method approaches often fall short due to the complexity and variability of weed problems like development of resistance in weeds. Therefore, an integrated weed management approach becomes effective, combining timely interventions with adaptive strategies to manage weed competition throughout the growing season. This approach not only enhances crop competitiveness and yield but also offers economic and environmental benefits by reducing reliance on broad-spectrum herbicides and promoting sustainable practices. Continuous monitoring and adjustment
of weed management strategies are crucial to effectively tackle evolving weed populations and changing conditions, ensuring long-term success in grain legume cultivation.
Keywords: Broad-spectrum herbicides, Crop productivity, Crop weed competition, Grain legume, Integrated weed management

Introduction
Aquaculture is one of the food-producing industries with the fastest rate of growth in the world and is widely recognized for its substantial contributions to food security, revenue production, and poverty alleviation (Subhasinghe, 2003). The production of aquaculture has increased dramatically during the last 30 years, from less than 20 million tonnes in the early 1990s to 178.9 million tonnes in 2022. The aquaculture industry reported an average growth rate of 6.7% per year globally from 1990 to 2020 (FAO, 2022). In 2020-22, the global annual per capita fish consumption was 20.4 kg (FAO, 2022). In addition, 58.5 million people were engaged in this sector worldwide for their livelihood. Many new approaches and technologies have been developed to increase the production. However, aquaculture faces significant challenges, including environmental quality, quality seed availability, and disease management (Sitcha et al., 2020; Oglend, 2020). To address these issues, the aquaculture industry adopts new scientific and technological advancements. Among the recent advancements in science, nanotechnology is fast emerging as a novel tool for the next generation of aquaculture and fisheries development. Nanotechnology is rapidly becoming a new tool for the growth of aquaculture and fisheries in the next generation, among other recent scientific breakthroughs
(Shah et al., 2020).

Introduction
Aquaculture has significantly contributed to the increase in global fish production. However, the domestication of only a limited number of species for intensive aquaculture has been achieved in recent decades. A primary challenge in the domestication and sustainable culture of candidate species lies in the standardization of induced breeding techniques. Controlled reproduction in captivity remains suboptimal for many commercially important aquaculture species. In captivity, females of several species often fail to complete oocyte maturation (Corriero et al., 2021), while males exhibit inadequate milt production (Zohar et al., 1989; Peter et al., 1993). Hormonal manipulation and environmental stimulation are considered effective approaches to overcome these challenges and facilitate captive maturation for induced breeding.
Several hormone analogues, such as gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), and luteinizing hormone-releasing hormone (LHRH), have been developed into commercial formulations like Ovaprim™, Ovatide™, and Wova-FH™, which target the brain-pituitarygonad (BPG) axis. However, these hormones have shown limited efficacy in the induced breeding of some commercially important species, including catfish. Recent studies suggest that neurohypophysial nonapeptide hormones also play a crucial role in modulating the GnRH– onadotropin (GTH)–steroid hormone pathway (Joy and Chaube, 2015). In mammals, nonapeptides include oxytocin (OT) and vasopressin (VP), which are homologous to isotocin (IT) and vasotocin (VT) in teleost fish. These nonapeptides, consisting of nine amino acids, are classified as basic (VT and VP) or neutral (OT and IT), depending on the amino acid at the eighth position. For example, VP has arginine, whereas OT has leucine at this position (Stoop, 2012).
In Heteropneustes fossilis, a novel neuropeptide gene, savetocin, has been identified, distinguished from IT by the substitution of valine for isoleucine at the eighth position. Nonapeptides also have homologues in other organisms, such as annetocin in worms and insects, inotocin in mollusks, and conopressin in leeches and Lymnaea stagnalis. Among vertebrates, lungfish, amphibians,
reptiles, and birds produce mesotocin and vasotocin, whereas marsupials secrete oxytocin and phenypressin (Darlison and Richter, 1999). These findings highlight the diversity and evolutionary significance of nonapeptides,
as well as their potential as novel targets for improving induced breeding in
aquaculture species.

Introduction
Global aquaculture plays a significant role in ensuring food security both directly (by making food more accessible and available) and indirectly (by promoting economic growth). Since the 1980s, aquaculture production has increased, and it has been suggested that there is theoretically enormous potential for further growth. FAO came to the conclusion that the industry should acknowledge the pertinent environmental and social issues as it continues to grow, intensify, and diversify. Climate change, and more especially the greenhouse gas (GHG) emissions that occur along food supply chains, is one of the major environmental (and social) problems. We must comprehend how aquaculture contributes to global GHG emissions and how to reduce them in order to support its sustainable growth (FAO, 2019).
What are Green House Gases?
Heat-trapping gases in the earth’s atmosphere are referred to as greenhouse gases, or GHGs. Maintaining a temperature on Earth that is conducive to life depends on greenhouse gases. The average temperature on Earth would be around -20°C if the greenhouse effect didn’t exist. Since the beginning of the Industrial Revolution, human activity has raised the atmospheric quantities
of greenhouse gases. The major GHG’s emitted into the earth’s atmosphere is given in Fig. 1.

Introduction
Temperature regulation is a critical physiological process that allows animals and birds to maintain homeostasis in varying environmental conditions. Thermoregulation involves a complex interplay of behavioral, physiological, and biochemical mechanisms that ensure the body temperature of these animals remains within a narrow, optimal range. This process is vital for maintaining metabolic functions, health, and productivity, particularly in agricultural settings where animals are often exposed to temperature extremes.In farm animals such as cattle, sheep, and pigs, thermoregulation is primarily managed through a combination of heat production, heat dissipation, and behavioral adaptations. Endothermic animals generate heat internally through metabolic processes, particularly in the muscles and liver. This heat is distributed throughout the body via the circulatory system. When ambient temperatures rise, farm animals activate various cooling mechanisms to prevent hyperthermia. These mechanisms include increased respiratory rates (panting), sweating (in species like cattle and horses), and vasodilation, where blood vessels near the skin surface expand to dissipate heat. Conversely, in cold environments, animals reduce heat loss by vasoconstriction, piloerection (raising of hair or fur), and seeking shelter or huddling together. Behavioral adaptations, such as seeking shade or water, also play a crucial role in temperature regulation.In poultry, thermoregulation is equally important but is managed differently due to their unique physiology. Birds lack sweat glands and have a higher metabolic rate compared to mammals, which means they rely more heavily on respiratory evaporative cooling and behavioral adaptations. Poultry regulate their body temperature by panting, which increases evaporative heat loss from the respiratory tract. Additionally, birds adjust their posture, such as spreading their wings or ruffling feathers, to enhance heat dissipation. During cold stress, poultry increase their metabolic rate, fluff their feathers to trap air for insulation, and reduce blood flow to peripheral areas to conserve heat. The thermal comfort zone, or thermoneutral zone, is narrower in poultry compared to larger mammals, making them more susceptible to temperature extremes.The ability of farm animals and poultry to regulate their body temperature is influenced by several factors, including age, breed, body condition, and acclimatization to the environment. Younger animals and certain breeds are more vulnerable to temperature stress due to their less developed thermoregulatory mechanisms or specific genetic traits. For instance, breeds with higher muscle mass or thicker coats may be more prone to heat stress, while those with lower body fat reserves may struggle in cold conditions. Acclimatization, or the gradual adaptation to a new thermal environment, plays a key role in enhancing the thermoregulatory capacity of these animals.

Introduction
Toxic inorganic and organic compounds are continuously contaminating our environment, endangering both human health and the planet’s ecosystems. This is a major worldwide problem. Because they persist in the environment, these pollutants which are caused by industrial processes, agricultural practices, urbanization, and over use of natural resources are very hard to control. While inorganic contaminants, including heavy metals, are difficult to break down naturally and need more complex remediation techniques, organic molecules may frequently be broken down into less dangerous components. Among these methods, bioremediation has become a viable, environmentally beneficial option that uses the inherent powers of fungus, bacteria, and plants to purify
and repair polluted soils and streams.
Phytoremediation, a specific form of bioremediation, harnesses the power of green plants to absorb, accumulate, degrade, or stabilize these harmful substances (Wang et al., 2021). This process not only mitigates pollution but also revitalizes ecosystems by improving soil structure, increasing biodiversity, and supporting sustainable land use. Phytoremediation provides a low-cost
and less intrusive substitute for conventional cleanup techniques, which frequently entail high-energy procedures, chemical treatments, or considerable excavation, as the globe struggles with the growing danger of environmental deterioration. Rapid industrialisation, urban waste, and natural activities can introduce large quantities of highly toxic organic pollutants, including
petroleum hydrocarbons, nitro and halogenated aromatic compounds, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), dibenzofurans and dibenzop-dioxins (PCDD/Fs), solvents, explosives, and pesticides, into both aquatic and soil ecosystems (Tanwir et al., 2021). One of the major challenges to reducing water pollution is the presence of heavy metals including cadmium (Cd), lead (Pb), and chromium (Cr) in industrial and agricultural runoff. Associated with other pollutants, these metals disrupt aquatic ecosystems at the species and community levels, which has an array of negative impacts on animal and human health. The World Health Organization (WHO) has emphasized how important it is to keep an eye on these chemicals in drinking water because of their link to serious health concerns including cancer, respiratory disorders, and cardiovascular illnesses.

Introduction
In the intricate tapestry of life, the relationship between foodstuffs and animal nutrition forms a fundamental thread, weaving its way through the intricate patterns of ecological balance, agricultural practices, and the sustenance of diverse life forms. The intersection of these two realms is not only pivotal for the flourishing of animals but also crucial for the well-being of ecosystems, human societies, and the planet at large. This intricate connection delves into the nuanced interplay between dietary components, physiological mechanisms, and the broader implications for the health of animals, both domestic and wild.
It is imperative to recognize that foodstuffs are not mere sustenance; they are the building blocks of life, the fuel for growth, and the source of energy that sustains the diverse array of creatures inhabiting our planet. From the microscopic organisms in the soil to the majestic mammals roaming vast landscapes, the quest for nourishment is a universal constant, intricately tied to the delicate balance of ecosystems.
At the heart of this relationship lies the fascinating science of animal nutrition—a field that scrutinizes the complex interactions between dietary elements and an organism’s physiological functions. From the intricacies of nutrient absorption to the role of different foodstuffs in promoting health, animal nutrition serves as a compass guiding us through the intricate pathways of biology, agriculture, and environmental conservation. The journey into understanding animal nutrition commences with a closer look at the varied foodstuffs that contribute to the dietary landscape. Foodstuffs, in this context, encompass a vast array of substances ranging from grains and forage to proteins and fats, each playing a distinctive role in the nutritional well-being of animals. The composition and quality of these foodstuffs are crucial determinants of an animal’s growth, reproduction, and overall
health. Unraveling the nutritional value of these components requires delving into the realms of biochemistry, physiology, and the intricate dance between macronutrients and micronutrients.
Macroscopic entities such as carbohydrates, proteins, and lipids stand as the pillars of animal nutrition. Carbohydrates, in the form of sugars, starches, and fibers, serve as the primary source of energy, fueling the metabolic processes that sustain life. Proteins, comprising amino acids, are the essential building blocks for growth, development, and the maintenance of bodily functions. Meanwhile, lipids, including fats and oils, play
multifaceted roles, serving as energy reserves, structural components, and facilitators of nutrient absorption.

Introduction
Pesticides play a crucial role in agriculture. During the period of the Green Revolution, the use of pesticides increased dramatically (Matson et al., 1997). According to the Environmental Protection Agency (EPA), pesticides can be defined as compounds that are used for the destruction, repulsion, or prevention of organisms that can be harmful to cultured crops (pests) as well as affect the regulation of plant growth. They are categorized based on several factors, such as their chemical composition (organophosphates, pyrethroids, neonicotinoids, etc.), based on the types of pests they eradicate (herbicides, insecticides, fungicides, etc.), and application methods. Although each pesticide has a distinct purpose, they all have comparable advantages. Some of the primary benefits of pesticide usage are the prevention of disease andan increase in yield, whereas secondary benefits include economic efficiency, an increase in food quality, facilitation of sustainable agriculture, improving public health, etc. However, the extensive use of pesticides has its consequences. These pesticides are meant to control or eliminate pests, but their toxicity could end up in unexpected areas. Its utilization poses significant harm to the environment, particularly to aquatic ecosystems. While they are essential in agriculture, their unregulated application leads to environmental degradation. The level of toxicity is influenced by factors such as pesticide concentration, mode of action, chemical composition, and persistence in the environment. Pesticides can cause both acute as well as chronic health effects. Through runoff or leaching, they indirectly enter water bodies, harming aquatic flora and fauna. A 2001 study revealed that over 90 percent of fish and water samples collected from various streams contained traces of one or more pesticides (Kole et al., 2001).

Introduction
Heart failure can develop as a complication of almost all congenital and acquired cardiac diseases in dogs and cats. Cardiac disease is a significant global health concern, impacting both humans and domestic animals, particularly dogs. In the animal kingdom, the cardiovascular system consists of essential components, including the heart, veins, arteries, and capillary beds. Its proper function depends on complex mechanisms, such
as the atrioventricular valves (mitral and tricuspid) and the semilunar valves (aortic and pulmonic), which ensure that blood flows in only one direction through the heart. The activity of the cardiovascular system, including heart contraction rates and strengths, as well as blood vessel diameter adjustments, is regulated by the autonomic nervous system (both sympathetic and parasympathetic branches) and various hormones.
The strength of ventricular contractions is influenced by several factors, primarily enddiastolic volume (preload) and myocardial contractility (inotropy). Preload is the amount of blood in the ventricles just before they contract, while contractility refers to the ability of the heart muscle fibers to contract. Preload is determined by the difference between the end-diastolic pressure in the ventricle and the pressure in the pleural space, adjusted for the
stiffness of the ventricular myocardium. This pressure is influenced by the ratio of blood volume to the heart muscle’s compliance. Low-pressure volume receptors in the heart and large veins primarily regulate preload. When these receptors detect increased blood volume or vein distension, they trigger responses such as increased urine production and vein dilation, which help reduce blood volume and lower venous pressures. Increased enddiastolic volume (preload) stretches the ventricular wall, enhancing contraction strength through the Frank-Starling mechanism, or Starling’s law of the heart. This stretching activates receptors in the atria and ventricles, leading to the release of natriuretic peptides like brain natriuretic peptide (BNP) from the ventricles and atrial natriuretic peptide (ANP) from the atria) These peptides promote natriuresis, relax smooth muscle, and counteract
vasopressin and angiotensin II. In dogs, levels of BNP and NT-proBNP are directly related to cardiac stretch.

Introduction
In cattle, the digestive system consists of four primary compartments: the rumen, reticulum, omasum, and abomasum, followed by the small and large intestines. The rumen plays a crucial role as a fermentation chamber, maintaining anaerobic conditions and ensuring thorough mixing, as well as regulating temperature and pH levels. Food, which is frequently re-chewed, enters the rumen through the esophagus, where fermentation occurs.
The byproducts of fermentation are either absorbed within the rumen or moved on for further digestion and absorption in other parts of the digestive system. Simple indigestion in ruminants is a temporary disruption in gastrointestinal function, usually caused by a sudden change in the quality or quantity of their diet. Consuming large amounts of indigestible feed can impair rumen function for 24 to 48 hours after ingestion, as the accumulation of this feed can physically disrupt ruminal activity. Additionally, the breakdown of proteins in the rumen can produce toxic substances such as amides and amines, including histamine. This condition can lead to a significant drop in milk production due to a marked reduction in the production of volatile fatty acids in a hypotonic reticulorumen. Ruminal impaction is a condition seen in ruminants like cows, sheep,and goats, where the  rumen’s contents become compacted, obstructing their movement through the digestive tract. This condition can arise from various causes, including the ingestion of plastic waste, grazing habits, mineral deficiencies, negative energy balance, urbanization, inadequate water intake, low-fiber or high-grain diets, changes in feed or grazing patterns, and stress. The accumulation of foreign material can block fermentation, interfere with the mixing of rumen contents, and disrupt the rumen’s microflora, leading to indigestion. Acute ruminal acidosis is often caused by the sudden intake of large amounts of carbohydrate-rich feed, particularly grains. Subacute ruminal acidosis (SARA) occurs in dairy cattle and is characterized by disrupted ruminal fermentation. It typically results from feeding large amounts of concentrates with insufficient fiber, a practice often used to boost milk production during early lactation. This imbalance in diet can disturb rumen pH and microbial populations, leading to various metabolic and digestive problems. Chronic ruminal acidosis can lead to complications such as rumenitis, which is inflammation of the rumen, and laminitis, a painful condition affecting the hooves. Alkaline indigestion, also known as ruminal alkalosis, occurs when the pH of ruminal fluid rises above 7.5.
 

Introduction
Respiratory diseases are a common clinical issue in dogs, particularly in those housed in environments like pet shops, shelters, breeding and boarding kennels. Puppies and senior dogs are at a higher risk of developing respiratory infections compared to adult dogs. The respiratory system is one of the body’s four major systems, comprising the upper airways (pharynx, larynx, and trachea and the lower airways (bronchi, bronchioles, and
alveoli). The pharynx, which serves as a common passageway for both the respiratory and gastrointestinal systems, explains the relatively high incidence of aspiration pneumonia. The luminal diameter of the pharynx is the widest, followed by the larynx and trachea.
The ratio of tracheal diameter to thoracic inlet height (TD:TI) remains relatively constant, regardless of the animal’s size or respiratory phase. The respiratory tract is typically protected from harmful pathogens by physical, chemical, and immunologicbarriers, including mucus, mucociliary clearance, innate antimicrobial factors, alveolar macrophages, and the pulmonary immune response. The entire respiratory tree is lined with ciliated epithelial cells, except for the bronchioles, which are lined by non-ciliated granular secretory cells known as club cells. The respiratory system’s primary function is achieved through four major processes: pulmonary ventilation; diffusion of oxygen and carbon dioxide between the alveoli and the blood; transport of these gases in the blood and body fluids to and from the body’s tissue cells; and regulation of ventilation and other respiratory factors. The pulmonary alveoli, the main sites for gas exchange between air and blood, are highly vulnerable to injury if the body’s defense mechanisms are compromised, underscoring the importance of prompt emergency treatment for respiratory diseases

Introduction
In a host-parasite relationship, the interaction between the two organisms is highly specialized. Each partner impacts the other’s life by influencing their metabolism and behavior through various adaptive mechanisms, all aimed at ensuring their own survival. While the parasite exerts every effort to fully establish itself within the host—by consuming its resources, damaging its tissues, secreting toxins, and evading the host’s immune
system through molecular mimicry—the host, in turn, strives to eliminate the parasite by mobilizing its immune defenses.
Despite their ongoing conflict, neither the host nor the parasite is able to completely eradicate the other. The host seeks to fend off the harmful, invasive parasite, while the parasite aims to establish a lasting, close relationship with the host. However, this dynamic can lead to a point where either the host or the parasite, often the parasite, becomes dominant or independent, resulting in the death of one party. Thus, the host-parasite relationship appears to be a perpetual and interdependent one, each partner intricately tied to the other. When a parasite enters a host, the host must make adjustments, and the parasite must adapt
to the host’s environment. This interaction leads to the development of a relationship in which both the host and the parasite influence each other’s growth, metabolism, and overall well-being. Generally, the host-parasite relationship unfolds through a series of stages: it starts with the transmission of the parasite from one host to another, followed by the parasite’s distribution and localization within or on the new host. This is followed by the
parasite’s growth or multiplication. Throughout this process, both the host and the parasite engage in resistance efforts against each other. The process involves several stages: the parasite’s method of attack, the changes the parasite induces in the host, and the alterations the parasite undergoes due to its presence in the host. It also includes the adjustments made by both host and parasite during the infection, the escape of the parasite’s infective stages from the host, and ultimately, the host’s recovery or death

Introduction
Animal behavior, a fascinating field of study, encompasses a wide range of activities and responses to internal and external stimuli. Among the various factors that shape these behaviour’s, hormones and drugs are particularly significant. These biochemical agents profoundly influence the physiological and psychological aspects of animals, leading to observable changes in their behaviour. This introduction explores the roles of hormones and drugs in animal behavior, detailing their mechanisms, effects, and implications for broader scientific understanding and practical applications. Hormones and drugs play vital roles in shaping animal behavior through complex biochemical pathways. Hormones act as internal regulators, orchestrating responses to environmental and physiological changes, while drugs, whether therapeutic or environmental, modulate these processes, often with profound behavioral consequences. Understanding these influences is crucial for advancing animal welfare, conservation efforts, and biomedical research, ultimately enriching our comprehension of the intricate relationship between biology and behavior in the animal kingdom.
Hormones and Animal Behaviour Deliver
Hormones are chemical messengers produced by endocrine glands and secreted into the bloodstream, where they travel to target organs and tissues, eliciting specific physiological responses. These responses, in turn, influence behaviour. The following are some key hormones that play critical roles in animal behavior:
1. Corticosteroids
• Function: Corticosteroids, such as cortisol in mammals and corticosterone in birds and reptiles, are released by the adrenal glands in response to stress.
• Behavioral Impact: They prepare the body for the fight-or-flight response, affecting behaviour’s related to fear, anxiety, and aggression. Chronic stress exposure and elevated corticosteroid levels can lead to long-term behavioral changes, including heightened anxiety and altered social interactions.

Evolution of Economics as a Discipline
1. Initially, economics was not a separate subject but a part of politics, ethics, and jurisprudence.
2. The study of economic ideas gained prominence through discussions on wealth, trade, and resource management.
3. Adam Smith’s book, An Inquiry into the Nature and Causes of the Wealth of Nations (1776), established economics as a distinct discipline.
4. Due to his contributions, Adam Smith is regarded as the Father of Economics.
Origin of the Term “Economics”
1. The word Economics is derived from the Greek term “Oikonomikos”:
a) Oikos – Household
b) Nomos – Management
2. Originally, Oikonomikos referred to the management of household affairs.

Approaches to study subject matter of economics
There are two approaches to study subject matter of economics-
1. Traditional Approach
2. Modern Approach
Economics studies human economic activities, which involve the creation, use, exchange, and distribution of wealth.
1. Traditional Approach
This approach splits economic activities into four interconnected areas:
Consumption, Production, Exchange, and Distribution (CPED).
1. Consumption – Using Goods & Services
Consumption refers to the utilization of goods and services to satisfy human needs and wants. It involves the destruction of utility, meaning that once a good or service is consumed, it cannot be reused in its original form i.e. once used, their original form is gone (utility destroyed) Economics studies the nature of human wants, their characteristics, and how individuals make consumption decisions.

Production- Function- The technical law relating inputs to outputs has beengiven the name of production function. Production function expresses the relationship between physical input and physical output of a firm for a given state of technology.
Law of Variable Proportion or Law of Diminishing Returns (Short Run Production Function)
This law explains the relationship between inputs and outputs in the short period. According to this law, output can be changed by altering variable factors (such as labour and feed) while keeping other factors (such as land and infrastructure) constant. The law states that with an increase in variable factors, keeping other factors constant, total product initially increases at an increasing rate, then increases at a diminishing rate, and finally starts decreasing.
1. Total Product (TP) - Refers to the total output of the firm per period of time.
1. Example: Total litres of milk produced by a dairy farm per day.

Abstract
Aquatic ecosystems are seriously threatened by heavy metal pollution from urban, agricultural, and industrial processes. Aquatic animals can accumulate toxic metals like mercury, lead, cadmium, arsenic and chromium, which can have detrimental physiological, ecological, and toxicological impacts. Oxidative stress, organ damage, poor reproduction, and disruption of ecosystem functioning, including loss of biodiversity and changes in food web dynamics, can all result from exposure to these contaminants. Human health is also at risk from heavy metals moving via aquatic food networks. The effects of heavy metal pollution on aquatic life are examined in this chapter, along with mitigation strategies.
Keywords: Heavy metals, pollution, toxicity, aquatic ecosystems and mitigation strategies

Introduction
Diabetes mellitus is a disease the related to metabolism of the body and malfunctioning of pancreas. Pancreas having alpha and beta cells where alpha cells produce enzymes needed for the digestion and beta cells mainly produce insulin. One important component that regulates blood glucose (sugar) levels and how it is delivered to the body’s cells is Insulin. The body’s cells run out of their primary energy source when insulin levels are low. Insulin voices the body’s cells to engross glucose from the bloodstream. Without an satisfactory amount of insulin glucose cannot invade the cells and so it accrues in the blood, triggering hyperglycemia (high blood sugar). In retort to this ostensible starvation, the body starts flouting down supplies of protein & fat for energy, producing weight loss contempt a ravening appetite. The body efforts to remove the extra glucose by micturating. To avoid thirst, the dog drinks more and more water. Type I increased diabetes mellitus outcomes from completeobliterat ion of the insulin-creating beta cells. In dogs, this is the most prevalent kind of diabetes. As the name implies, insulin injections are necessary to balance blood sugar in dogs with this kind of diabetes.

Viral Diseases in Domestic Animals
Viral diseases significantly impact the health and productivity of domestic animals, including livestock and companion animals, leading to substantial economic and emotional costs. In livestock, diseases like Foot-and-Mouth Disease (FMD) and Peste des Petits Ruminants (PPR) cause severe production losses in terms of milk, meat, and wool. Similarly, in poultry, Newcastle disease and Avian Influenza outbreaks result in high mortality and disruptions to egg production. Among companion animals, viruses such as Canine Distemper and Feline Leukemia Virus (FeLV) lead to serious morbidity and reduced quality of life. Current control measures rely heavily on vaccination and biosecurity practices, but the lack of effective antiviral treatments, coupled with the emergence of drug-resistant strains, underscores the need
for novel therapeutic approaches. Medicinal plants with antiviral properties have garnered significant interest as sustainable and holistic solutions. Plantderived bioactive compounds offer a dual advantage of modulating immune responses and directly targeting viral pathogens, making them a promising area of research for the management of viral diseases in both livestock and pet animals.

Cardiovascular System Disorders in Domestic Animals
Cardiovascular system disorders in domestic animals include a range ofM conditions that affect the heart (pericardium, myocardium, endocardium), veins, arteries and lymphatic vessels. Infectious disorders like Heartworms (Dirofilaria immitis, vegetative endocarditis in pigs, equine viral arteritis). Patency of the foramen ovale, interventricular foramena, patent ductus arteriosus, persistence of the right aortic arch, coarctation of the aorta, transposition of the aorta, congenital aneurysm, pulmonic stenosis, subaortic stenosis, ectopia cardis, acardia, and diplocardia are examples of congenital and developmental disorders that can result in these conditions. Traumatic disorders like Traumatic reticulopericarditis and cardiac failure. Degenerative diseases like fatty and hyaline degeneration. Inflammatory disorders like pericarditis,
myocarditis, endocarditis and phlebitis may also lead to cardiovascular system disorders. Some tumours associated with the cardiovascular system like rhabdomyosarcoma, fibroma and myxofibroma also cause cardiovascular system disorders.

Abstract
Single-cell proteins (SCPs) have gained huge attention in the aquafeed industry as a suitable alternative to traditional protein sources, including fish meal and soy protein. SCPs obtained from the algae, bacteria, fungi, and yeast often contain high protein content and superior amino acid profiles. The presence of essential fatty acids and additional bioactive compounds further support their role as adequate replacements for the current protein sources. Among those, mycoproteins are currently a major area of interest due to their favourable nutritional profile, higher growth rates and efficient production process. These have the ability to use a variety of agricultural and industrial by-products as their feedstocks. PEKILO® protein is such a commercial mycoprotein obtained from carbohydrates containing lignocellulosic wastes from forestry by-products. It was produced from the fungal species Paecilomyces variotii from the substrates such as spent sulphite liquor and molasses through a continuous fermentation process. Pekilo protein consists of 60 to 70% crude protein content, essential amino acids, vitamins, minerals, β-glucans and nucleotides. Its high digestibility and amino acid content make it a suitable protein source for the aquafeed industry. Recent research studies suggest that pekilo protein has a high potential as a non-conventional protein source in commercial salmon and shrimp feeds. Being a sustainable alternative to fishmeal, pekilo protein can help reduce the future challenges of fish meal supply and long-term sustainability.
Keywords: Pekilo protein, Mycoprotein, Non-conventional ingredient and Single-cell protein.

Abstract
The development of aquaculture from its prehistoric beginnings to a highly developed, international sector emphasizes the need for cutting-edge equipment to guarantee sustained production. Passive Integrated Transponder (PIT) tagging is a cornerstone for selective breeding programs, enabling precise monitoring and managing aquatic species. PIT tags, tiny electronic microchips implanted into fish, offer a reliable and durable method for individual identification without affecting species growth or survival. Their versatility extends across various applications, from studying migration and behaviour to monitoring genetic traits critical for breeding. PIT tagging stands out due to its simplicity, cost-effectiveness, and compatibility with various aquatic environments. PIT tags offer a long-term solution with less interference than external tags, which are more likely to be lost or damaged. Technological advancements have refined PIT tagging, allowing its application even in small-bodied fish, thus broadening its utility in ecological and genetic studies. These tags also play a pivotal role in reducing inbreeding in hatcheries by maintaining precise genetic records. Despite the proven benefits, PIT tagging requires careful consideration of fish species, size, and environmental factors to optimize tag retention and ensure minimal stress. Advancements in tagging techniques, such as improved surgical techniques and new equipment for tag implantation further increase the dependability of this technology. Studies confirm that PIT tagging has negligible effects on fish physiology and behaviour, making it an indispensable tool in aquaculture research and management. The aquaculture sector can enhance broodstock quality, guarantee genetic diversity, and tackle climate change issues by incorporating PIT tagging into selective breeding strategies. This technology not only supports the production of high-quality seed stock but also aligns
with sustainable aquaculture practices, ensuring the long-term viability and growth of the industry.
Keywords: Tag retention, Biotelemetry, Tag implanter, Tag position and Broodstock

Abstract
The growing demand for fish products, recognized for their nutritional composition and contribution to a healthy diet, has led to a significant increase in seafood processing waste, including shells, bones, skins, and other by-products. This chapter explores the potential of utilizing these seafood by-products to develop edible films and coatings, aligning with circular bio economy principles of, resource efficiency, sustainability and waste reduction. Edible films and coatings derived from seafood waste, such as chitosan, proteins, gelatin etc. offer various advantages, which include extention of the shelf life of products, preserving sensory qualities (aroma, taste, and appearance), and reducing the need for synthetic packaging materials. These biodegradable films also demonstrate antimicrobial, antioxidant, and mould-inhibiting properties, which contribute to improving food quality and safety. However, despite the benefits, challenges remain in terms of the physical properties of these films, including water resistance, mechanical strength, and consumer acceptance. The commercial application of these biopolymer-based materials is still limited by these issues, as well as by the complexities involved in scaling up production processes. To optimise the qualities of edible films and coatings made from seafood waste, more research is essential, particularly through the development of composite materials and innovative gelling systems. With ongoing advancements in biopolymer technology and processing techniques, edible films from seafood waste hold great promise as a sustainable, eco-friendly alternative to traditional plastic packaging, contributing to the circular bio economy and reducing environmental pollution.
Keywords: Biodegradable, Edible coatings, Edible films, Seafood Processing waste

Introduction
Environmental Impact Assessment (EIA) is a process of evaluating the likely environmental impacts of a proposed project or development, taking into account inter-related socio-economic, cultural and human-health impacts, both beneficial and adverse.
UNEP defines Environmental Impact Assessment (EIA) as a tool used to identify the environmental, social and economic impacts of a project prior to decision-making. It aims to predict environmental impacts at an early stage in project planning and design, find ways and means to reduce adverse impacts, shape projects to suit the local environment and present the predictions and
options to decision-makers.
Environment Impact Assessment in India is statutorily backed by the Environment Protection Act, 1986 which contains various provisions on EIA methodology and process.
Evolution & History of EIA
EIA is termed as one of the best policy innovations in the 1900s. The main aim of EIA is to conserve the environment and bring out the best combination of economic and environmental costs and benefits. Read the below-mentioned points to understand the Environmental Impact Assessment evolution and history:
• The birth of EIA is dated back to the 1970s.

Policy research plays a critical role in influencing policy change by generating robust evidence that can guide decision-making processes. The use of evidence is crucial in ensuring that policies are both effective and appropriate to address specific issues. Evidence-based policy-making relies on credible, relevant and timely research that helps identify problems, evaluate potential solutions and
forecast the impacts of various policy options.
Role of Policy Research in Generating Evidence
1. Identification of issues: Policy research begins with identifying key issues or challenges that require policy intervention. This involves conducting systematic research to understand the scope, scale and
nature of the problem. For example, research on gender disparities in agricultural labor in India may highlight specific barriers women face, such as lack of access to resources or discriminatory practices.
2. Data collection and analysis: Gathering quantitative and qualitative data is essential for building a comprehensive understanding of the issue. For instance, data collection may involve surveys, interviews,
case studies and secondary data analysis to capture diverse perspectives and experiences. This data provides a solid foundation for analyzing the problem’s root causes and identifying the affected population.

19.1 Introduction
Mulberry silkworm, Bombyx mori, is affected by a number of diseases caused by viruses, bacteria, fungi and protozoa. These illnesses are recognized to occur in practically every region where silkworms are raised in the world seriously harming the harvest of silkworm cocoons. Several actions have been proposed as a means of preventing and managing the illnesses, but none of them had shown signs of foolproof with the outcome that one must consistently be cautious to eradicate the core infection’s source in addition to avoiding crossinfection. Additionally, care has to be taken in order to ensure that they are not exposed to stressful circumstances such as low temperature and inadequate nourishment, poor ventilation, and humidity which could lead them easily susceptible to various diseases.
19.2 Diseases of Mulberry Silkworm and Their Control
19.2.1 Protozoan Diseases
The protozoa that harm silkworms are the parasitic ones from the genera Nosema, Pleistophora, and Thelohania and class Microsporidia. In addition to this, reports of Leptomonas and Trypanosoma infections in the silkworm
Bombyx mori L. have been made. However, the pebrine disease is the main protozoan illness of the silkworm. It is also known as ‘pepper disease’ because of the development of areas of black pepper after infection.

Cereal grains and cereal by-products
The appropriate nutrition of livestock necessitates the inclusion of a specific proportion of cereals and their byproducts. For poultry and swine, cereals constitute the primary element of their daily dietary intake. Consequently, there exists a direct competition for these cereal resources between livestock and humans. Specifically, only 2% of rice and wheat, 5% of sorghum, 10% of barley and maize, and 50% of bajra and ragi are allocated for animal feed.
Cereals belong to the Gramineae family and are classified as annual plants (Khariff). These grains are primarily composed of carbohydrates, with starch being the predominant component of their dry matter, concentrated in the endosperm. Cereal grains are abundant in TDN and net energy, making them highly palatable to livestock. The DCP content ranges from 7% to 10%, while TDN levels fall between 70% and 80% for ruminants. In non-ruminants, such as pigs and poultry, these energy-rich grains can be incorporated into their diets at proportions of approximately 50% to 70%.
Structure of a grain: Grain kernels are referred to as caryopses. Those grains that possess husks, such as oats, barley and rice, are classified as covered caryopses, while grains that lack husks, including maize, wheat, rye, and sorghum, are termed naked caryopses. Each kernel, excluding the husk, consists of two primary components: the pericarp and the seed.
Pericarp: This structure is composed of two distinct layers. The outer layer, which includes the epidermis and hydroderm, is collectively known as the beeswing. The inner layer of the pericarp is characterized by the presence of cross cells and tube cells.
Seed: The seed is further divided into four components: the seed coat, hyaline layer, endosperm, and germ (embryo). When the grain undergoes processing that removes both the germ and the starch endosperm, the remaining parts of the seed along with the pericarp are referred to as bran.

Plants and many agro-industrial by-products incorporated into livestock diets may harbor a range of anti-nutritional compounds. These substances can adversely affect optimal nutrition by deactivating specific nutrients, obstructing the digestive process, or diminishing the metabolic efficiency of feed, ultimately leading to negative consequences for animal health and productivity. The naturally occurring plant compounds found in feed that disrupt feed utilization (FCR), lower production levels, and compromise animal health are referred to as anti-nutritional factors. Additionally, natural toxicants present in feed can hinder the absorption and utilization of nutrients in livestock. Therefore, it is essential to implement effective detoxification methods for these feed materials to mitigate toxic effects and ensure their safe incorporation into animal diets. A foundational understanding of these toxicants is crucial prior to undertaking detoxification and subsequent use in livestock feeding.
Natural toxicants can be categorized based on their chemical properties into several groups: Proteins, Glycosides, Phenols, and Miscellaneous toxicants. The chemical characteristics, pathogenic effects, and detoxification strategies for these compounds are briefly outlined below.
Proteins
1. Protease inhibitors: Protease inhibitors are prevalent throughout the plant kingdom, particularly in the seeds of many cultivated legumes. These inhibitors possess the capability to obstruct the function of proteolytic enzymes in the gastrointestinal systems of animals. Specifically, trypsin inhibitors form irreversible complexes with trypsin, rendering the enzyme unable to fulfill its role in protein digestion. This interaction prompts the intestine to secrete cholecystokinin, which in turn stimulates pancreatic enlargement. The presence of trypsin inhibitors in raw soybean can hinder the availability of methionine, leading to adverse effects in young chickens fed raw soybean meal,
including pancreatic hypertrophy, reduced growth rates, gallbladder contraction and increased bile acid excretion, similar to observations in other monogastric animals. In contrast, ruminants can effectively utilize untreated leguminous seeds. Consequently, the amino acids that would typically be released by trypsin are not reabsorbed, leading to their loss when trypsin interacts with these inhibitors. These substances, which are proteinaceous in nature, hinder the activity of certain digestive enzymes, ultimately impairing the growth and performance of non-ruminant animals. High concentrations of protease inhibitors are found in various leguminous seeds, such as soybean, kidney bean and mung bean. There are two primary types of protease inhibitors: a) Kunitz inhibitors, which possess a single active site that binds exclusively to trypsin and b) Bowman-Birk inhibitors, which contain two active sites that can bind both trypsin and chymotrypsin, thereby inhibiting both enzymes. Detoxification: These protease inhibitors are primarily heat-sensitive and appropriate heat treatment of leguminous seeds-such as roasting, toasting, popping, autoclaving (at 120°C and 15 lbs psi for 20 minutes), and cooking-can effectively inactivate them. It is important to avoid overheating during these processes.

Phosphorus is a critical mineral for animal health, playing a vital role in bone development, energy metabolism, cell signalling, and overall physiological functions. Disorders related to phosphorus imbalance, whether due to deficiency or excess, can lead to significant health issues in animals.
a) Pica
Pica is a condition characterized by the persistent ingestion of non-nutritive substances such as soil, rocks, plastic, cloth, or other inappropriate materials. While commonly observed in humans, pica is also prevalent in animals, including livestock, domestic pets, and wildlife. It is often a sign of underlying health, environmental, or nutritional issues. Understanding the causes, implications, and management of pica is crucial for animal welfare and productivity.
Causes of pica in animals
The aetiology of pica in animals is multifaceted and can be broadly categorized into nutritional, medical, behavioural, and environmental factors.